AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0267170

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0267170 MtrunA17_Chr5g0424931 93.182 528 35 1 1 527 1 528 0.0 1013
Msa0267170 MtrunA17_Chr1g0178011 54.598 522 160 8 8 525 1 449 4.33e-177 506
Msa0267170 MtrunA17_Chr5g0417281 49.817 546 207 11 14 527 6 516 5.97e-159 462
Msa0267170 MtrunA17_Chr5g0436761 48.846 520 187 10 14 521 7 459 3.39e-152 443
Msa0267170 MtrunA17_Chr5g0424741 47.426 544 188 13 8 479 1 518 8.24e-143 421
Msa0267170 MtrunA17_Chr3g0115271 44.524 557 255 15 13 526 13 558 5.26e-142 422
Msa0267170 MtrunA17_Chr2g0277461 47.868 516 185 13 16 523 10 449 5.23e-136 401
Msa0267170 MtrunA17_Chr5g0424801 53.299 394 139 10 8 391 1 359 5.74e-130 383
Msa0267170 MtrunA17_Chr5g0425261 53.708 391 142 9 8 391 1 359 3.92e-129 381
Msa0267170 MtrunA17_Chr2g0289221 53.714 350 118 9 6 346 7 321 5.15e-117 349
Msa0267170 MtrunA17_Chr5g0417211 42.667 525 162 12 15 526 8 406 4.80e-116 348
Msa0267170 MtrunA17_Chr2g0326761 39.364 503 246 14 19 477 11 498 1.23e-99 315
Msa0267170 MtrunA17_Chr1g0181211 40.249 482 226 12 14 451 22 485 8.01e-99 313
Msa0267170 MtrunA17_Chr7g0264171 38.445 476 218 13 14 437 26 478 3.32e-96 304
Msa0267170 MtrunA17_Chr1g0199681 40.260 462 226 14 7 431 82 530 8.44e-94 300
Msa0267170 MtrunA17_Chr4g0028971 40.265 452 216 12 19 431 12 448 1.37e-92 297
Msa0267170 MtrunA17_Chr2g0277531 46.512 344 131 12 15 346 16 318 1.38e-86 270
Msa0267170 MtrunA17_Chr5g0425661 60.606 198 70 3 16 208 34 228 1.99e-78 246
Msa0267170 MtrunA17_Chr7g0264161 40.606 330 154 9 29 346 2 301 1.88e-70 227
Msa0267170 MtrunA17_Chr2g0282961 33.075 387 201 9 1 366 38 387 5.03e-65 222
Msa0267170 MtrunA17_Chr2g0285461 35.250 400 209 12 6 384 6 376 3.35e-60 213
Msa0267170 MtrunA17_Chr2g0276781 35.185 378 208 7 19 385 23 374 9.38e-60 209
Msa0267170 MtrunA17_Chr4g0070181 34.140 372 196 7 14 368 18 357 1.41e-59 213
Msa0267170 MtrunA17_Chr8g0385791 33.943 383 207 9 19 385 59 411 2.35e-59 210
Msa0267170 MtrunA17_Chr5g0433301 34.704 389 202 11 16 384 21 377 5.18e-59 209
Msa0267170 MtrunA17_Chr4g0046371 34.501 371 188 10 19 366 38 376 7.40e-59 207
Msa0267170 MtrunA17_Chr3g0109391 33.505 388 208 9 16 384 23 379 9.90e-59 209
Msa0267170 MtrunA17_Chr2g0301251 33.875 369 201 8 14 368 18 357 1.40e-58 210
Msa0267170 MtrunA17_Chr8g0376771 34.085 399 214 11 6 384 6 375 7.32e-58 207
Msa0267170 MtrunA17_Chr1g0156321 34.783 368 196 9 14 366 38 376 1.22e-57 206
Msa0267170 MtrunA17_Chr4g0009751 34.646 381 207 10 19 385 19 371 1.55e-57 202
Msa0267170 MtrunA17_Chr8g0389081 34.239 368 201 9 16 369 29 369 1.50e-55 201
Msa0267170 MtrunA17_Chr7g0239061 30.958 449 263 13 19 448 14 434 1.08e-54 195
Msa0267170 MtrunA17_Chr2g0326281 32.921 404 206 13 19 388 56 428 6.83e-54 195
Msa0267170 MtrunA17_Chr4g0029671 34.271 391 205 10 19 385 54 416 1.05e-52 191
Msa0267170 MtrunA17_Chr8g0346711 33.333 378 212 9 19 385 29 377 1.76e-52 189
Msa0267170 MtrunA17_Chr5g0425651 72.321 112 27 1 263 370 1 112 1.23e-49 166
Msa0267170 MtrunA17_Chr5g0424791 47.020 151 64 5 68 213 12 151 3.13e-31 118
Msa0267170 MtrunA17_Chr8g0356211 26.446 242 155 3 125 366 2 220 1.00e-22 99.0
Msa0267170 MtrunA17_Chr5g0419861 39.130 115 57 4 57 163 12 121 6.16e-14 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0267170 AT1G77850.1 38.609 575 286 14 1 526 1 557 3.06e-121 369
Msa0267170 AT1G77850.2 38.609 575 286 14 1 526 1 557 3.63e-121 369
Msa0267170 AT4G30080.1 38.739 555 275 20 19 526 19 555 1.29e-94 302
Msa0267170 AT2G28350.2 42.079 404 191 11 19 388 10 404 1.17e-86 281
Msa0267170 AT2G28350.1 42.079 404 191 11 19 388 10 404 1.57e-86 281
Msa0267170 AT2G46530.1 36.667 360 194 8 18 369 20 353 9.65e-64 219
Msa0267170 AT2G46530.3 36.695 357 192 8 18 366 41 371 1.48e-63 219
Msa0267170 AT2G33860.1 34.877 367 188 8 19 366 54 388 8.64e-62 213
Msa0267170 AT5G37020.2 33.747 403 216 11 16 399 19 389 2.85e-61 215
Msa0267170 AT3G61830.1 34.667 375 201 9 15 381 21 359 5.14e-61 211
Msa0267170 AT5G37020.1 33.747 403 216 11 16 399 19 389 5.78e-61 215
Msa0267170 AT1G30330.3 34.625 387 209 9 14 384 20 378 5.63e-60 213
Msa0267170 AT1G30330.2 34.625 387 209 9 14 384 20 378 5.63e-60 213
Msa0267170 AT1G30330.1 34.625 387 209 9 14 384 18 376 6.12e-60 213
Msa0267170 AT5G60450.2 34.271 391 203 13 13 382 35 392 1.38e-57 205
Msa0267170 AT5G20730.2 33.957 374 194 8 14 368 19 358 2.65e-57 207
Msa0267170 AT5G60450.1 34.278 388 201 13 16 382 62 416 3.14e-57 204
Msa0267170 AT5G62000.4 32.412 398 225 9 3 385 45 413 5.55e-57 204
Msa0267170 AT5G62000.2 32.412 398 225 9 3 385 45 413 6.96e-57 204
Msa0267170 AT5G62000.5 32.412 398 225 9 3 385 45 413 6.96e-57 204
Msa0267170 AT5G62000.3 32.412 398 225 9 3 385 45 413 6.96e-57 204
Msa0267170 AT5G62000.1 32.412 398 225 9 3 385 45 413 6.96e-57 204
Msa0267170 AT1G59750.4 33.884 363 204 6 19 371 22 358 9.34e-57 201
Msa0267170 AT1G59750.2 33.884 363 204 6 19 371 22 358 9.82e-57 201
Msa0267170 AT1G59750.1 33.884 363 204 6 19 371 22 358 1.21e-56 201
Msa0267170 AT1G59750.3 33.884 363 204 6 19 371 22 358 1.21e-56 201
Msa0267170 AT4G23980.2 30.309 485 283 14 19 483 12 461 1.84e-56 199
Msa0267170 AT1G19220.1 33.850 387 208 11 16 385 20 375 2.48e-56 204
Msa0267170 AT4G23980.1 33.594 384 213 10 19 389 12 366 3.25e-56 199
Msa0267170 AT5G20730.1 33.867 375 194 9 14 368 19 359 1.17e-55 202
Msa0267170 AT5G20730.3 33.867 375 194 9 14 368 19 359 1.30e-55 202
Msa0267170 AT1G19850.2 34.426 366 197 9 16 366 40 377 4.26e-55 199
Msa0267170 AT1G19850.1 34.426 366 197 9 16 366 51 388 6.22e-55 199
Msa0267170 AT1G34310.1 32.987 385 215 9 16 389 24 376 2.36e-54 193
Msa0267170 AT1G35520.1 33.681 383 209 11 19 389 27 376 1.62e-53 191
Msa0267170 AT1G34410.1 31.990 397 226 11 19 405 27 389 2.28e-53 191
Msa0267170 AT1G34390.1 32.041 387 216 10 19 397 27 374 4.71e-53 189
Msa0267170 AT1G35540.1 31.842 380 220 9 19 389 27 376 5.25e-51 184
Msa0267170 AT1G35240.1 31.552 393 219 11 19 398 27 382 1.22e-50 183
Msa0267170 AT3G61830.2 34.337 332 178 8 54 381 13 308 1.85e-47 173
Msa0267170 AT1G34170.1 31.148 366 196 10 19 366 25 352 6.42e-46 168
Msa0267170 AT2G33860.2 34.241 257 135 4 117 366 2 231 3.47e-45 165
Msa0267170 AT2G46530.2 36.965 257 137 4 114 369 34 266 6.20e-44 163
Msa0267170 AT5G37020.3 34.589 292 154 8 117 399 31 294 4.08e-42 160
Msa0267170 AT1G34170.3 26.923 520 270 17 19 507 25 465 2.39e-40 154
Msa0267170 AT1G34170.2 29.508 366 176 10 19 366 25 326 1.52e-39 150
Msa0267170 AT1G43950.1 38.049 205 111 6 19 216 27 222 1.42e-36 135
Msa0267170 ATMG00940.1 34.586 133 75 4 204 327 1 130 7.02e-15 72.8

Find 149 sgRNAs with CRISPR-Local

Find 260 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTGGTTCAATTAAGTTATT+TGG 0.154755 2_2:-68624097 Msa0267170:CDS
GGCACATGTCTGCCAGATTT+TGG 0.193688 2_2:+68627283 None:intergenic
TTCAAACTACTAAGGTTATT+TGG 0.200926 2_2:+68624175 None:intergenic
TCTCTTTATCATAGGTTAAC+TGG 0.215844 2_2:-68624552 Msa0267170:intron
CCCTAATTGGCGCATGCTTC+AGG 0.273598 2_2:-68626322 Msa0267170:intron
AGAGAATGGTTTGGAGGTTA+AGG 0.274717 2_2:-68626625 Msa0267170:CDS
CATGATCATTTATCTCTATC+AGG 0.276134 2_2:-68624283 Msa0267170:CDS
TGAACCAAACATTGTTGAAT+TGG 0.283162 2_2:-68624339 Msa0267170:CDS
TATATTACCCTAGGGCTAAT+TGG 0.305719 2_2:-68626503 Msa0267170:CDS
AATTTCATTGGATCCTTTAG+AGG 0.319231 2_2:+68624015 None:intergenic
GTCAATGCATCAAATTTCAT+TGG 0.331745 2_2:+68624003 None:intergenic
CTGATGTTCATGACGGAGTT+TGG 0.334825 2_2:-68626857 Msa0267170:CDS
TACCGGACTGAGTAGCAATT+TGG 0.335469 2_2:+68627073 None:intergenic
AGCCCCAATTCAACAATGTT+TGG 0.335857 2_2:+68624335 None:intergenic
ACGGTGATTGTTTGAGCGTT+AGG 0.337529 2_2:+68627179 None:intergenic
ATGAAGATTGGTTGGGCATC+TGG 0.343084 2_2:-68626441 Msa0267170:CDS
ACCTGAAGCATGCGCCAATT+AGG 0.343626 2_2:+68626321 None:intergenic
GAACCAAACATTGTTGAATT+GGG 0.349855 2_2:-68624338 Msa0267170:CDS
AGTTCTTGTCCTTCAGTTAC+CGG 0.352672 2_2:+68627056 None:intergenic
CTTAGTAGTTTGAACACATT+CGG 0.352705 2_2:-68624166 Msa0267170:CDS
GAGAATGGTTTGGAGGTTAA+GGG 0.354789 2_2:-68626624 Msa0267170:CDS
CAAACAATCACCGTTATTGA+TGG 0.359311 2_2:-68627170 Msa0267170:CDS
TAAACTTTGGAACGTACCTT+TGG 0.368148 2_2:+68627242 None:intergenic
GAAAGTGATGTCCATGATTC+TGG 0.371511 2_2:-68624055 Msa0267170:CDS
GGAGAATTTAAGTCAAGCTT+GGG 0.377595 2_2:+68626906 None:intergenic
CCTGAAGCATGCGCCAATTA+GGG 0.379672 2_2:+68626322 None:intergenic
CGGTGATTGTTTGAGCGTTA+GGG 0.384846 2_2:+68627180 None:intergenic
TGCCTCAATAACCGCCTTCT+CGG 0.388165 2_2:+68626563 None:intergenic
GAGTCCAACCAGTAGTAAAA+AGG 0.388502 2_2:+68626796 None:intergenic
TCTTTGAATGATAATAGTTA+TGG 0.390167 2_2:-68624250 Msa0267170:CDS
GGAGTTGGCGGAAAAGAATA+TGG 0.402969 2_2:-68626538 Msa0267170:CDS
CTCTTTATCATAGGTTAACT+GGG 0.403608 2_2:-68624551 Msa0267170:intron
GGGACAGACATGGTGGCCGG+TGG 0.403787 2_2:+68627326 None:intergenic
TAGATGCACTGCAGGATTGC+GGG 0.405787 2_2:+68624468 None:intergenic
CAGCAGGCATGCAGGGAGTC+AGG 0.416086 2_2:-68624306 Msa0267170:CDS
CATCACGGCCGTTGATCTCC+TGG 0.419001 2_2:-68627123 Msa0267170:CDS
ATGTGTCCAGTGTCCCTAAT+TGG 0.419261 2_2:-68626335 Msa0267170:CDS
TTAGTAGTTTGAACACATTC+GGG 0.421729 2_2:-68624165 Msa0267170:CDS
ACTATTATCATTCAAAGAGT+TGG 0.428670 2_2:+68624255 None:intergenic
ATCAAAACCGCGTCAATCCT+TGG 0.437926 2_2:-68624507 Msa0267170:CDS
GCCGCGTGAAATCCAAAATC+TGG 0.443335 2_2:-68627295 Msa0267170:CDS
TTGAAGTTATATATTACCCT+AGG 0.447607 2_2:-68626512 Msa0267170:CDS
AGGAGAATTTAAGTCAAGCT+TGG 0.457295 2_2:+68626905 None:intergenic
CGCCAAATTGCTACTCAGTC+CGG 0.457882 2_2:-68627075 Msa0267170:CDS
CTGACTCCCTGCATGCCTGC+TGG 0.459021 2_2:+68624307 None:intergenic
ACAAAATCTGATTCTAATAA+CGG 0.462788 2_2:-68626972 Msa0267170:CDS
AAGGAGGAGGGTAAAGAGAA+TGG 0.463485 2_2:-68626639 Msa0267170:CDS
GAACTCTATCATAGGAATGA+AGG 0.464627 2_2:+68624375 None:intergenic
TTGGATTTCACGCGGCGTGC+CGG 0.466164 2_2:+68627302 None:intergenic
GGTTTGGAGGTTAAGGGAGA+TGG 0.469959 2_2:-68626618 Msa0267170:CDS
CTCTGAGGGAACTCTATCAT+AGG 0.472447 2_2:+68624367 None:intergenic
AACACAAAAGGAGTTGGTTC+TGG 0.474124 2_2:-68624115 Msa0267170:CDS
GGAGGGTAAAGAGAATGGTT+TGG 0.480150 2_2:-68626634 Msa0267170:CDS
GTGGATGATGCTATGAAGAT+TGG 0.483610 2_2:-68626453 Msa0267170:CDS
AAGGGTGAAGATTCCTCTAA+AGG 0.485287 2_2:-68624028 Msa0267170:CDS
TTTAGAGGAATCTTCACCCT+TGG 0.485917 2_2:+68624030 None:intergenic
TCTTCTTTATCAACAACCTC+AGG 0.486494 2_2:+68627032 None:intergenic
GGTTATTGAGGCAGTGGAGT+TGG 0.491818 2_2:-68626553 Msa0267170:CDS
GAGAGTTCCTGAGACGGAAA+AGG 0.492404 2_2:+68626885 None:intergenic
TGGAACGTTCACCGATGGAC+CGG 0.495600 2_2:+68627262 None:intergenic
CGAATGTGTTCAAACTACTA+AGG 0.499098 2_2:+68624167 None:intergenic
TTACTTCCCACATGGTCACT+TGG 0.499922 2_2:-68627219 Msa0267170:CDS
GGCCACCATGTCTGTCCCTC+CGG 0.501925 2_2:-68627321 Msa0267170:CDS
TAAAGAAGAAGACGGTGATA+AGG 0.507483 2_2:-68627018 Msa0267170:CDS
GATTTCACGCGGCGTGCCGG+AGG 0.509842 2_2:+68627305 None:intergenic
ACCTTTGGAACGTTCACCGA+TGG 0.510835 2_2:+68627257 None:intergenic
TTCCGCCATGTCTTCCCCAC+CGG 0.514610 2_2:-68627342 None:intergenic
AAAATCTGGCAGACATGTGC+CGG 0.515291 2_2:-68627281 Msa0267170:CDS
GGACAGACATGGTGGCCGGT+GGG 0.516458 2_2:+68627327 None:intergenic
GCAGACATGTGCCGGTCCAT+CGG 0.518088 2_2:-68627273 Msa0267170:CDS
ATTGTTTGAGCGTTAGGGGA+AGG 0.519009 2_2:+68627185 None:intergenic
GTGAATAATAAGAAGGAGGA+GGG 0.519419 2_2:-68626651 Msa0267170:CDS
GGTGGCCGGTGGGGAAGACA+TGG 0.519543 2_2:+68627337 None:intergenic
AAAAGCAGTGAATAATAAGA+AGG 0.519919 2_2:-68626658 Msa0267170:CDS
ATGTGGGAAGTAATAAACTT+TGG 0.520720 2_2:+68627229 None:intergenic
ACTGCCGGAGATATAGTTGT+TGG 0.521728 2_2:-68626717 Msa0267170:CDS
AACCGAGAAGGCGGTTATTG+AGG 0.522496 2_2:-68626565 Msa0267170:CDS
ACTCACAACAACAACACAAA+AGG 0.522870 2_2:-68624127 Msa0267170:CDS
TCTGATTCTAATAACGGCGG+TGG 0.523119 2_2:-68626966 Msa0267170:CDS
GAAGATTCATCAATCTTCAA+AGG 0.524456 2_2:+68626402 None:intergenic
GTAGAATCCTGAACTAGTCT+AGG 0.524565 2_2:+68624424 None:intergenic
AGTGAATAATAAGAAGGAGG+AGG 0.524591 2_2:-68626652 Msa0267170:CDS
GACAGACATGGTGGCCGGTG+GGG 0.526151 2_2:+68627328 None:intergenic
GGGTAAAGAGAATGGTTTGG+AGG 0.526164 2_2:-68626631 Msa0267170:CDS
GACCTCATCGGTGTGAGGAT+CGG 0.526510 2_2:+68627100 None:intergenic
CACCCTTGGTACCAGAATCA+TGG 0.526655 2_2:+68624044 None:intergenic
CAGCAAACTTCATGTTTCTG+CGG 0.530328 2_2:+68626691 None:intergenic
TGTAGTTGATTCAAATGTTG+TGG 0.533977 2_2:-68626472 Msa0267170:CDS
GGAGGGACAGACATGGTGGC+CGG 0.536529 2_2:+68627323 None:intergenic
AACCAAACATTGTTGAATTG+GGG 0.537810 2_2:-68624337 Msa0267170:CDS
AACTCTATCATAGGAATGAA+GGG 0.541293 2_2:+68624376 None:intergenic
AGAAATGGTAGATGCACTGC+AGG 0.541883 2_2:+68624460 None:intergenic
GATTCCAACAACTATATCTC+CGG 0.544415 2_2:+68626713 None:intergenic
TACCTTGTTTAGTGATAACA+AGG 0.546334 2_2:-68624404 Msa0267170:CDS
CATCGGTGTGAGGATCGGCC+AGG 0.550560 2_2:+68627105 None:intergenic
TATTGAGGCAGTGGAGTTGG+CGG 0.551866 2_2:-68626550 Msa0267170:CDS
GAAATAAGTTCAACTTGCCA+AGG 0.552664 2_2:+68624490 None:intergenic
TCAACGGCCGTGATGATACA+AGG 0.557986 2_2:+68627131 None:intergenic
GAAGGCGGTTATTGAGGCAG+TGG 0.558765 2_2:-68626559 Msa0267170:CDS
TTGAGCGTTAGGGGAAGGGC+AGG 0.559568 2_2:+68627190 None:intergenic
TTGTTTGAGCGTTAGGGGAA+GGG 0.559742 2_2:+68627186 None:intergenic
ATGATGCTATGAAGATTGGT+TGG 0.562627 2_2:-68626449 Msa0267170:CDS
TTTGGTTCAAAGATCTCTGA+GGG 0.562864 2_2:+68624353 None:intergenic
TGAAGTTATATATTACCCTA+GGG 0.565837 2_2:-68626511 Msa0267170:CDS
TGGCGACAAGTCGTTAGATG+TGG 0.568778 2_2:+68624195 None:intergenic
ATTTCACGCGGCGTGCCGGA+GGG 0.569870 2_2:+68627306 None:intergenic
AAATTGCACCAATTAGCCCT+AGG 0.571867 2_2:+68626495 None:intergenic
ATCATGGACATCACTTTCAA+CGG 0.572291 2_2:+68624060 None:intergenic
GTTGTTGATAAAGAAGAAGA+CGG 0.573045 2_2:-68627026 Msa0267170:CDS
CTACTCAGTCCGGTAACTGA+AGG 0.578098 2_2:-68627065 Msa0267170:CDS
TGATGCTATGAAGATTGGTT+GGG 0.580467 2_2:-68626448 Msa0267170:CDS
AAGGGGAATGCTAACCGAGA+AGG 0.592212 2_2:-68626577 Msa0267170:CDS
GTAGATGCACTGCAGGATTG+CGG 0.593419 2_2:+68624467 None:intergenic
GTGATGATACAAGGAAAAGA+AGG 0.595656 2_2:+68627140 None:intergenic
GGCGACAAGTCGTTAGATGT+GGG 0.596585 2_2:+68624196 None:intergenic
AACAACAACACAAAAGGAGT+TGG 0.596832 2_2:-68624121 Msa0267170:CDS
TGTCCATGATTCTGGTACCA+AGG 0.599100 2_2:-68624047 Msa0267170:CDS
CATGCGCCAATTAGGGACAC+TGG 0.600814 2_2:+68626329 None:intergenic
TCCATCGGTGAACGTTCCAA+AGG 0.605082 2_2:-68627258 Msa0267170:CDS
AAGTTTGCTCATGTCTATCG+AGG 0.613801 2_2:-68626834 Msa0267170:CDS
AGGACGGTAACCATCAATAA+CGG 0.618603 2_2:+68627160 None:intergenic
GCATGTTCCAAGTGACCATG+TGG 0.620759 2_2:+68627212 None:intergenic
GTCCATGATTCTGGTACCAA+GGG 0.620974 2_2:-68624046 Msa0267170:CDS
AGGATCGGCCAGGAGATCAA+CGG 0.621476 2_2:+68627115 None:intergenic
ACCCTTGTTATCACTAAACA+AGG 0.625546 2_2:+68624402 None:intergenic
GGCCGATCCTCACACCGATG+AGG 0.626120 2_2:-68627102 Msa0267170:CDS
CAACTTGCCAAGGATTGACG+CGG 0.626664 2_2:+68624500 None:intergenic
AGCAGTGAATAATAAGAAGG+AGG 0.629221 2_2:-68626655 Msa0267170:CDS
GGTGATTGTTTGAGCGTTAG+GGG 0.631012 2_2:+68627181 None:intergenic
CAATTTGGCGAAGACCTCAT+CGG 0.632488 2_2:+68627088 None:intergenic
AATTGCACCAATTAGCCCTA+GGG 0.639117 2_2:+68626496 None:intergenic
GCGTGCCGGAGGGACAGACA+TGG 0.644657 2_2:+68627316 None:intergenic
ATCAAGTCAGCACAAAGGCG+AGG 0.645484 2_2:+68626933 None:intergenic
GTTTGGTTCAAAGATCTCTG+AGG 0.646990 2_2:+68624352 None:intergenic
GCGAAGACCTCATCGGTGTG+AGG 0.652471 2_2:+68627095 None:intergenic
GGGAATGCTAACCGAGAAGG+CGG 0.652518 2_2:-68626574 Msa0267170:CDS
AGTTTGCTCATGTCTATCGA+GGG 0.654001 2_2:-68626833 Msa0267170:CDS
AAAGTTTATTACTTCCCACA+TGG 0.657806 2_2:-68627227 Msa0267170:CDS
ACATTAGAGATTGTTCCTTG+AGG 0.666803 2_2:+68626354 None:intergenic
TGATACAAGGAAAAGAAGGA+CGG 0.677808 2_2:+68627144 None:intergenic
TCTGTTACTGATGTTCATGA+CGG 0.679070 2_2:-68626864 Msa0267170:CDS
TGCCGGAGGGACAGACATGG+TGG 0.679386 2_2:+68627319 None:intergenic
TGAAGGACAAGAACTTCCTG+AGG 0.686819 2_2:-68627048 Msa0267170:CDS
GTAACAGAGAGTTCCTGAGA+CGG 0.687969 2_2:+68626879 None:intergenic
GAGAATTTAAGTCAAGCTTG+GGG 0.690174 2_2:+68626907 None:intergenic
CATGTTCCAAGTGACCATGT+GGG 0.696959 2_2:+68627213 None:intergenic
AAATCTGATTCTAATAACGG+CGG 0.706742 2_2:-68626969 Msa0267170:CDS
GGAAGATCAAGTCAGCACAA+AGG 0.715164 2_2:+68626928 None:intergenic
ACCTTGTTTAGTGATAACAA+GGG 0.724822 2_2:-68624403 Msa0267170:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTTATTTTTTGAATCT+AGG - chr2_2:68625077-68625096 Msa0267170:intron 10.0%
!!! TATTTTATTTTTTGAATCTA+GGG - chr2_2:68625078-68625097 Msa0267170:intron 10.0%
!! CAATTTAGTATATAAATATT+AGG - chr2_2:68626105-68626124 Msa0267170:intron 10.0%
!!! AGAATACTTTTTTTTTTTTT+TGG - chr2_2:68626327-68626346 Msa0267170:CDS 10.0%
!!! TGCATTTTATTATAAAAATA+TGG + chr2_2:68626637-68626656 None:intergenic 10.0%
!! ATAGTTTAGTATACTTAATT+AGG + chr2_2:68625293-68625312 None:intergenic 15.0%
!!! ATTTTACATTAATAATTGCT+TGG + chr2_2:68625753-68625772 None:intergenic 15.0%
!!! AAAACATGATTTTTTAAACT+TGG + chr2_2:68626075-68626094 None:intergenic 15.0%
!! TTATATACTAAATTGATCAA+GGG + chr2_2:68626100-68626119 None:intergenic 15.0%
!! TTTATATACTAAATTGATCA+AGG + chr2_2:68626101-68626120 None:intergenic 15.0%
!!! CTATAATTATGGATTAATTT+TGG + chr2_2:68626417-68626436 None:intergenic 15.0%
!! ACAAAATCTGATTCTAATAA+CGG - chr2_2:68624375-68624394 Msa0267170:CDS 20.0%
!! TTGAAACTATAATCAAATGA+TGG - chr2_2:68625697-68625716 Msa0267170:intron 20.0%
!! AAATATTAGGGTTAAGTTAT+AGG - chr2_2:68626118-68626137 Msa0267170:intron 20.0%
!! TATTTATAACTTATCCGATA+GGG + chr2_2:68626173-68626192 None:intergenic 20.0%
!! TTATTTATAACTTATCCGAT+AGG + chr2_2:68626174-68626193 None:intergenic 20.0%
!!! GTCGTTAATTTACTATTTTT+TGG - chr2_2:68626224-68626243 Msa0267170:intron 20.0%
!!! TGGAAAAGTATTAGATATTT+AGG - chr2_2:68626608-68626627 Msa0267170:CDS 20.0%
!! CTATGATAAAGAGATAAAAA+TGG + chr2_2:68626789-68626808 None:intergenic 20.0%
!!! CATTTTTATCTCTTTATCAT+AGG - chr2_2:68626787-68626806 Msa0267170:CDS 20.0%
!! TCTTTGAATGATAATAGTTA+TGG - chr2_2:68627097-68627116 Msa0267170:CDS 20.0%
! AAAAGCAGTGAATAATAAGA+AGG - chr2_2:68624689-68624708 Msa0267170:intron 25.0%
! TTGAAGTTATATATTACCCT+AGG - chr2_2:68624835-68624854 Msa0267170:intron 25.0%
! TGAAGTTATATATTACCCTA+GGG - chr2_2:68624836-68624855 Msa0267170:intron 25.0%
! TATTATTGTCGAAGAAGTTT+AGG - chr2_2:68625132-68625151 Msa0267170:intron 25.0%
!! AGTGTAAAAAAAAGGTTTTC+GGG + chr2_2:68625377-68625396 None:intergenic 25.0%
!! GAGTGTAAAAAAAAGGTTTT+CGG + chr2_2:68625378-68625397 None:intergenic 25.0%
! GAAAAGTGAGTGTAAAAAAA+AGG + chr2_2:68625385-68625404 None:intergenic 25.0%
! TAAAGAGAAAGAAAAATCTC+CGG + chr2_2:68625504-68625523 None:intergenic 25.0%
!! ATTGCTTGGTATTAGAATTT+TGG + chr2_2:68625739-68625758 None:intergenic 25.0%
! AACATTATTCAACGATTATG+AGG - chr2_2:68625827-68625846 Msa0267170:intron 25.0%
! ATGATTACCAAAATACAGTT+AGG - chr2_2:68625851-68625870 Msa0267170:intron 25.0%
!! GATTTTTCCTAACTGTATTT+TGG + chr2_2:68625861-68625880 None:intergenic 25.0%
! GGTATATATGTTTAATCAAG+AGG - chr2_2:68625977-68625996 Msa0267170:intron 25.0%
! TATGTTTAATCAAGAGGAAT+AGG - chr2_2:68625983-68626002 Msa0267170:intron 25.0%
! ATGTTTAATCAAGAGGAATA+GGG - chr2_2:68625984-68626003 Msa0267170:intron 25.0%
! AAATTAACGACTAACTTAGT+TGG + chr2_2:68626215-68626234 None:intergenic 25.0%
! TTACCTTCAACACTAATAAA+AGG - chr2_2:68626259-68626278 Msa0267170:intron 25.0%
! TACCTTCAACACTAATAAAA+GGG - chr2_2:68626260-68626279 Msa0267170:intron 25.0%
! TTGCAGTTGATCTATAATTA+TGG + chr2_2:68626428-68626447 None:intergenic 25.0%
!!! GTTTTCAGGCATATAAATAA+TGG - chr2_2:68626565-68626584 Msa0267170:CDS 25.0%
! ATTTACTCTTATTCCTAAGT+AGG - chr2_2:68626656-68626675 Msa0267170:CDS 25.0%
! GTAAAATATAGATCCTACTT+AGG + chr2_2:68626672-68626691 None:intergenic 25.0%
! AGTATGTATATGCACATAAA+AGG + chr2_2:68626724-68626743 None:intergenic 25.0%
!!! ATATTTTGAATGACTGATGT+TGG - chr2_2:68626756-68626775 Msa0267170:CDS 25.0%
! ACTATTATCATTCAAAGAGT+TGG + chr2_2:68627095-68627114 None:intergenic 25.0%
! TTCAAACTACTAAGGTTATT+TGG + chr2_2:68627175-68627194 None:intergenic 25.0%
!! TCTGGTTCAATTAAGTTATT+TGG - chr2_2:68627250-68627269 Msa0267170:CDS 25.0%
ATGTGGGAAGTAATAAACTT+TGG + chr2_2:68624121-68624140 None:intergenic 30.0%
AAAGTTTATTACTTCCCACA+TGG - chr2_2:68624120-68624139 Msa0267170:CDS 30.0%
! TTCTTTTCCTTGTATCATCA+CGG - chr2_2:68624209-68624228 Msa0267170:CDS 30.0%
! GTTGTTGATAAAGAAGAAGA+CGG - chr2_2:68624321-68624340 Msa0267170:CDS 30.0%
! TTTTGTAAGAGTCTTTACGA+AGG + chr2_2:68624361-68624380 None:intergenic 30.0%
AAATCTGATTCTAATAACGG+CGG - chr2_2:68624378-68624397 Msa0267170:CDS 30.0%
!!! GTAGTAAAAAGGTGTCTTTT+GGG + chr2_2:68624543-68624562 None:intergenic 30.0%
!!! AGTAGTAAAAAGGTGTCTTT+TGG + chr2_2:68624544-68624563 None:intergenic 30.0%
! AAAAGACACCTTTTTACTAC+TGG - chr2_2:68624543-68624562 Msa0267170:intron 30.0%
!!! AGTTTTTTCGTGTTCACAAA+CGG + chr2_2:68624576-68624595 None:intergenic 30.0%
! TGTAGTTGATTCAAATGTTG+TGG - chr2_2:68624875-68624894 Msa0267170:intron 30.0%
GAAGATTCATCAATCTTCAA+AGG + chr2_2:68624948-68624967 None:intergenic 30.0%
!!! TCTTTTTTAGAGCTTCAAAG+AGG - chr2_2:68625452-68625471 Msa0267170:intron 30.0%
! GTGGAGCAAATAAATTTTGT+TGG + chr2_2:68625482-68625501 None:intergenic 30.0%
ACAAAATTTATTTGCTCCAC+CGG - chr2_2:68625482-68625501 Msa0267170:intron 30.0%
CTCTTATTCAACCTCTTAAT+TGG - chr2_2:68625525-68625544 Msa0267170:intron 30.0%
ACTAATACCCATGTAATTCA+TGG + chr2_2:68625592-68625611 None:intergenic 30.0%
TTGTGAATTGATACTTGTAC+CGG + chr2_2:68625643-68625662 None:intergenic 30.0%
TCAAGAGAATTATGTGACTA+TGG - chr2_2:68625674-68625693 Msa0267170:intron 30.0%
! GTTGAATAATGTTTTCAGCA+AGG + chr2_2:68625820-68625839 None:intergenic 30.0%
TAGGGAAGTTATAGATAACT+TGG - chr2_2:68626002-68626021 Msa0267170:intron 30.0%
!! TTCCCTTTTATTAGTGTTGA+AGG + chr2_2:68626265-68626284 None:intergenic 30.0%
! ATAAATAATGGAGAAGCTTC+AGG - chr2_2:68626577-68626596 Msa0267170:CDS 30.0%
! TAAATAATGGAGAAGCTTCA+GGG - chr2_2:68626578-68626597 Msa0267170:CDS 30.0%
TCTCTTTATCATAGGTTAAC+TGG - chr2_2:68626795-68626814 Msa0267170:CDS 30.0%
CTCTTTATCATAGGTTAACT+GGG - chr2_2:68626796-68626815 Msa0267170:CDS 30.0%
! TACCTTGTTTAGTGATAACA+AGG - chr2_2:68626943-68626962 Msa0267170:CDS 30.0%
! ACCTTGTTTAGTGATAACAA+GGG - chr2_2:68626944-68626963 Msa0267170:CDS 30.0%
AACTCTATCATAGGAATGAA+GGG + chr2_2:68626974-68626993 None:intergenic 30.0%
TGAACCAAACATTGTTGAAT+TGG - chr2_2:68627008-68627027 Msa0267170:CDS 30.0%
GAACCAAACATTGTTGAATT+GGG - chr2_2:68627009-68627028 Msa0267170:CDS 30.0%
AACCAAACATTGTTGAATTG+GGG - chr2_2:68627010-68627029 Msa0267170:CDS 30.0%
CATGATCATTTATCTCTATC+AGG - chr2_2:68627064-68627083 Msa0267170:CDS 30.0%
CTTAGTAGTTTGAACACATT+CGG - chr2_2:68627181-68627200 Msa0267170:CDS 30.0%
TTAGTAGTTTGAACACATTC+GGG - chr2_2:68627182-68627201 Msa0267170:CDS 30.0%
AATTTCATTGGATCCTTTAG+AGG + chr2_2:68627335-68627354 None:intergenic 30.0%
!! TAAACTTTGGAACGTACCTT+TGG + chr2_2:68624108-68624127 None:intergenic 35.0%
CAAACAATCACCGTTATTGA+TGG - chr2_2:68624177-68624196 Msa0267170:CDS 35.0%
TGATACAAGGAAAAGAAGGA+CGG + chr2_2:68624206-68624225 None:intergenic 35.0%
GTGATGATACAAGGAAAAGA+AGG + chr2_2:68624210-68624229 None:intergenic 35.0%
TCTTCTTTATCAACAACCTC+AGG + chr2_2:68624318-68624337 None:intergenic 35.0%
TAAAGAAGAAGACGGTGATA+AGG - chr2_2:68624329-68624348 Msa0267170:CDS 35.0%
GAGAATTTAAGTCAAGCTTG+GGG + chr2_2:68624443-68624462 None:intergenic 35.0%
GGAGAATTTAAGTCAAGCTT+GGG + chr2_2:68624444-68624463 None:intergenic 35.0%
AGGAGAATTTAAGTCAAGCT+TGG + chr2_2:68624445-68624464 None:intergenic 35.0%
TCTGTTACTGATGTTCATGA+CGG - chr2_2:68624483-68624502 Msa0267170:CDS 35.0%
! GTTTTCATGAAAAACACTGC+CGG - chr2_2:68624615-68624634 Msa0267170:intron 35.0%
GATTCCAACAACTATATCTC+CGG + chr2_2:68624637-68624656 None:intergenic 35.0%
AGCAGTGAATAATAAGAAGG+AGG - chr2_2:68624692-68624711 Msa0267170:intron 35.0%
AGTGAATAATAAGAAGGAGG+AGG - chr2_2:68624695-68624714 Msa0267170:intron 35.0%
GTGAATAATAAGAAGGAGGA+GGG - chr2_2:68624696-68624715 Msa0267170:intron 35.0%
TATATTACCCTAGGGCTAAT+TGG - chr2_2:68624844-68624863 Msa0267170:intron 35.0%
ATGATGCTATGAAGATTGGT+TGG - chr2_2:68624898-68624917 Msa0267170:intron 35.0%
! TGATGCTATGAAGATTGGTT+GGG - chr2_2:68624899-68624918 Msa0267170:intron 35.0%
!!! ATGACATTTTTTCAGCCTCA+AGG - chr2_2:68624978-68624997 Msa0267170:intron 35.0%
ACATTAGAGATTGTTCCTTG+AGG + chr2_2:68624996-68625015 None:intergenic 35.0%
! ATTCGCATTTCGCTTTGTTA+CGG - chr2_2:68625185-68625204 Msa0267170:intron 35.0%
AAGAATTCGATGCATGCATA+CGG - chr2_2:68625229-68625248 Msa0267170:intron 35.0%
CAATACTATGAATCGATGCA+AGG + chr2_2:68625264-68625283 None:intergenic 35.0%
!! AAAAAAAGGTTTTCGGGAGT+GGG + chr2_2:68625371-68625390 None:intergenic 35.0%
!! AAAAAAAAGGTTTTCGGGAG+TGG + chr2_2:68625372-68625391 None:intergenic 35.0%
ATCAAAGAGGACCAATTAAG+AGG + chr2_2:68625539-68625558 None:intergenic 35.0%
CCAATCTCCATGAATTACAT+GGG - chr2_2:68625582-68625601 Msa0267170:intron 35.0%
!! TTCGTAAAAGTACCACAAAC+CGG - chr2_2:68625621-68625640 Msa0267170:intron 35.0%
AATCATATGAGGAGCAAGAA+GGG + chr2_2:68626506-68626525 None:intergenic 35.0%
GTCAATCACTCAATCATATG+AGG + chr2_2:68626517-68626536 None:intergenic 35.0%
! AAAGAGTGACAAGTGTTTTC+AGG - chr2_2:68626551-68626570 Msa0267170:CDS 35.0%
GAAATAAGTTCAACTTGCCA+AGG + chr2_2:68626860-68626879 None:intergenic 35.0%
!!! GGCTTTTTTGTTGAGAGAAA+TGG + chr2_2:68626905-68626924 None:intergenic 35.0%
! AAAAAAGCCTAGACTAGTTC+AGG - chr2_2:68626916-68626935 Msa0267170:CDS 35.0%
! ACCCTTGTTATCACTAAACA+AGG + chr2_2:68626948-68626967 None:intergenic 35.0%
GAACTCTATCATAGGAATGA+AGG + chr2_2:68626975-68626994 None:intergenic 35.0%
! TTTGGTTCAAAGATCTCTGA+GGG + chr2_2:68626997-68627016 None:intergenic 35.0%
CGAATGTGTTCAAACTACTA+AGG + chr2_2:68627183-68627202 None:intergenic 35.0%
ACTCACAACAACAACACAAA+AGG - chr2_2:68627220-68627239 Msa0267170:CDS 35.0%
AACAACAACACAAAAGGAGT+TGG - chr2_2:68627226-68627245 Msa0267170:CDS 35.0%
ATCATGGACATCACTTTCAA+CGG + chr2_2:68627290-68627309 None:intergenic 35.0%
AGGACGGTAACCATCAATAA+CGG + chr2_2:68624190-68624209 None:intergenic 40.0%
AGTTCTTGTCCTTCAGTTAC+CGG + chr2_2:68624294-68624313 None:intergenic 40.0%
! AAATTCTCCTTTTCCGTCTC+AGG - chr2_2:68624455-68624474 Msa0267170:CDS 40.0%
! AAGTTTGCTCATGTCTATCG+AGG - chr2_2:68624513-68624532 Msa0267170:CDS 40.0%
! AGTTTGCTCATGTCTATCGA+GGG - chr2_2:68624514-68624533 Msa0267170:CDS 40.0%
! GACACCTTTTTACTACTGGT+TGG - chr2_2:68624547-68624566 Msa0267170:intron 40.0%
GAGTCCAACCAGTAGTAAAA+AGG + chr2_2:68624554-68624573 None:intergenic 40.0%
AACACGAAAAAACTCGTTGC+TGG - chr2_2:68624582-68624601 Msa0267170:intron 40.0%
! CAGCAAACTTCATGTTTCTG+CGG + chr2_2:68624659-68624678 None:intergenic 40.0%
! AGAGAATGGTTTGGAGGTTA+AGG - chr2_2:68624722-68624741 Msa0267170:intron 40.0%
! GAGAATGGTTTGGAGGTTAA+GGG - chr2_2:68624723-68624742 Msa0267170:intron 40.0%
AATTGCACCAATTAGCCCTA+GGG + chr2_2:68624854-68624873 None:intergenic 40.0%
AAATTGCACCAATTAGCCCT+AGG + chr2_2:68624855-68624874 None:intergenic 40.0%
GTGGATGATGCTATGAAGAT+TGG - chr2_2:68624894-68624913 Msa0267170:intron 40.0%
!!! CTTCAGGTGCTCTTCTTTTT+TGG - chr2_2:68625041-68625060 Msa0267170:intron 40.0%
! TTCGCATTTCGCTTTGTTAC+GGG - chr2_2:68625186-68625205 Msa0267170:intron 40.0%
AGAGAAAGAAAAATCTCCGG+TGG + chr2_2:68625501-68625520 None:intergenic 40.0%
CAAGTGTGTGAAGATCAAAG+AGG + chr2_2:68625552-68625571 None:intergenic 40.0%
TGATCTTCACACACTTGTCT+CGG - chr2_2:68625555-68625574 Msa0267170:intron 40.0%
GCCAATCTCCATGAATTACA+TGG - chr2_2:68625581-68625600 Msa0267170:intron 40.0%
CCCATGTAATTCATGGAGAT+TGG + chr2_2:68625585-68625604 None:intergenic 40.0%
!! TTGATACTTGTACCGGTTTG+TGG + chr2_2:68625636-68625655 None:intergenic 40.0%
! GTTTTCAGCAAGGAATGTGT+TGG + chr2_2:68625810-68625829 None:intergenic 40.0%
!! GTTGTGATTGTGAGCATTGA+AGG + chr2_2:68625892-68625911 None:intergenic 40.0%
!! CACTTTTGCTGCTTCTATTG+CGG + chr2_2:68625958-68625977 None:intergenic 40.0%
ACAATGATCAAGGCTCTCAA+GGG + chr2_2:68626048-68626067 None:intergenic 40.0%
AACAATGATCAAGGCTCTCA+AGG + chr2_2:68626049-68626068 None:intergenic 40.0%
ACTTGGAGCAACAATGATCA+AGG + chr2_2:68626058-68626077 None:intergenic 40.0%
! GGGTTAAGTTATAGGAGTGT+AGG - chr2_2:68626126-68626145 Msa0267170:intron 40.0%
!! ATAGCTTTGTTCTGCCCTAT+CGG - chr2_2:68626156-68626175 Msa0267170:intron 40.0%
CAATCATATGAGGAGCAAGA+AGG + chr2_2:68626507-68626526 None:intergenic 40.0%
! GTAGAATCCTGAACTAGTCT+AGG + chr2_2:68626926-68626945 None:intergenic 40.0%
! GTTTGGTTCAAAGATCTCTG+AGG + chr2_2:68626998-68627017 None:intergenic 40.0%
AGCCCCAATTCAACAATGTT+TGG + chr2_2:68627015-68627034 None:intergenic 40.0%
! AACACAAAAGGAGTTGGTTC+TGG - chr2_2:68627232-68627251 Msa0267170:CDS 40.0%
GAAAGTGATGTCCATGATTC+TGG - chr2_2:68627292-68627311 Msa0267170:CDS 40.0%
TTTAGAGGAATCTTCACCCT+TGG + chr2_2:68627320-68627339 None:intergenic 40.0%
AAGGGTGAAGATTCCTCTAA+AGG - chr2_2:68627319-68627338 Msa0267170:CDS 40.0%
! GCCAGATTTTGGATTTCACG+CGG + chr2_2:68624056-68624075 None:intergenic 45.0%
AAAATCTGGCAGACATGTGC+CGG - chr2_2:68624066-68624085 Msa0267170:CDS 45.0%
TTACTTCCCACATGGTCACT+TGG - chr2_2:68624128-68624147 Msa0267170:CDS 45.0%
CATGTTCCAAGTGACCATGT+GGG + chr2_2:68624137-68624156 None:intergenic 45.0%
TTGTTTGAGCGTTAGGGGAA+GGG + chr2_2:68624164-68624183 None:intergenic 45.0%
ATTGTTTGAGCGTTAGGGGA+AGG + chr2_2:68624165-68624184 None:intergenic 45.0%
GGTGATTGTTTGAGCGTTAG+GGG + chr2_2:68624169-68624188 None:intergenic 45.0%
CGGTGATTGTTTGAGCGTTA+GGG + chr2_2:68624170-68624189 None:intergenic 45.0%
ACGGTGATTGTTTGAGCGTT+AGG + chr2_2:68624171-68624190 None:intergenic 45.0%
CAATTTGGCGAAGACCTCAT+CGG + chr2_2:68624262-68624281 None:intergenic 45.0%
TACCGGACTGAGTAGCAATT+TGG + chr2_2:68624277-68624296 None:intergenic 45.0%
TGAAGGACAAGAACTTCCTG+AGG - chr2_2:68624299-68624318 Msa0267170:CDS 45.0%
TCTGATTCTAATAACGGCGG+TGG - chr2_2:68624381-68624400 Msa0267170:CDS 45.0%
GGAAGATCAAGTCAGCACAA+AGG + chr2_2:68624422-68624441 None:intergenic 45.0%
GTAACAGAGAGTTCCTGAGA+CGG + chr2_2:68624471-68624490 None:intergenic 45.0%
! CTGATGTTCATGACGGAGTT+TGG - chr2_2:68624490-68624509 Msa0267170:CDS 45.0%
ACTGCCGGAGATATAGTTGT+TGG - chr2_2:68624630-68624649 Msa0267170:intron 45.0%
AAGGAGGAGGGTAAAGAGAA+TGG - chr2_2:68624708-68624727 Msa0267170:intron 45.0%
GGAGGGTAAAGAGAATGGTT+TGG - chr2_2:68624713-68624732 Msa0267170:intron 45.0%
! GGGTAAAGAGAATGGTTTGG+AGG - chr2_2:68624716-68624735 Msa0267170:intron 45.0%
! AAGGGAGATGGTTTTTCTCG+AGG - chr2_2:68624741-68624760 Msa0267170:intron 45.0%
GGAGTTGGCGGAAAAGAATA+TGG - chr2_2:68624809-68624828 Msa0267170:intron 45.0%
! ATGAAGATTGGTTGGGCATC+TGG - chr2_2:68624906-68624925 Msa0267170:intron 45.0%
ATGTGTCCAGTGTCCCTAAT+TGG - chr2_2:68625012-68625031 Msa0267170:intron 45.0%
! TTCGCTTTGTTACGGGTTTG+CGG - chr2_2:68625193-68625212 Msa0267170:intron 45.0%
AATTCGATGCATGCATACGG+AGG - chr2_2:68625232-68625251 Msa0267170:intron 45.0%
!! ATGTGTTGGTTGAGCTGAGA+TGG + chr2_2:68625796-68625815 None:intergenic 45.0%
CGCAATAGAAGCAGCAAAAG+TGG - chr2_2:68625956-68625975 Msa0267170:intron 45.0%
! AGAAGCTTCAGGGTGTCTTA+TGG - chr2_2:68626588-68626607 Msa0267170:CDS 45.0%
ATCAAAACCGCGTCAATCCT+TGG - chr2_2:68626840-68626859 Msa0267170:CDS 45.0%
AGAAATGGTAGATGCACTGC+AGG + chr2_2:68626890-68626909 None:intergenic 45.0%
! CTCTGAGGGAACTCTATCAT+AGG + chr2_2:68626983-68627002 None:intergenic 45.0%
!! AATTGGGGCTATTTTCCAGC+AGG - chr2_2:68627025-68627044 Msa0267170:CDS 45.0%
! TATTTTCCAGCAGGCATGCA+GGG - chr2_2:68627034-68627053 Msa0267170:CDS 45.0%
TGTCCATGATTCTGGTACCA+AGG - chr2_2:68627300-68627319 Msa0267170:CDS 45.0%
GTCCATGATTCTGGTACCAA+GGG - chr2_2:68627301-68627320 Msa0267170:CDS 45.0%
!! TAATATTACAAAAAATTAAA+AGG - chr2_2:68625315-68625334 Msa0267170:intron 5.0%
!! AATTTAGTATATAAATATTA+GGG - chr2_2:68626106-68626125 Msa0267170:intron 5.0%
GCCGCGTGAAATCCAAAATC+TGG - chr2_2:68624052-68624071 Msa0267170:CDS 50.0%
! GGCACATGTCTGCCAGATTT+TGG + chr2_2:68624067-68624086 None:intergenic 50.0%
TCCATCGGTGAACGTTCCAA+AGG - chr2_2:68624089-68624108 Msa0267170:CDS 50.0%
!! ACCTTTGGAACGTTCACCGA+TGG + chr2_2:68624093-68624112 None:intergenic 50.0%
GCATGTTCCAAGTGACCATG+TGG + chr2_2:68624138-68624157 None:intergenic 50.0%
TCAACGGCCGTGATGATACA+AGG + chr2_2:68624219-68624238 None:intergenic 50.0%
CGCCAAATTGCTACTCAGTC+CGG - chr2_2:68624272-68624291 Msa0267170:CDS 50.0%
CTACTCAGTCCGGTAACTGA+AGG - chr2_2:68624282-68624301 Msa0267170:CDS 50.0%
ATCAAGTCAGCACAAAGGCG+AGG + chr2_2:68624417-68624436 None:intergenic 50.0%
GAGAGTTCCTGAGACGGAAA+AGG + chr2_2:68624465-68624484 None:intergenic 50.0%
GGTTTGGAGGTTAAGGGAGA+TGG - chr2_2:68624729-68624748 Msa0267170:intron 50.0%
! GGAGATGGTTTTTCTCGAGG+CGG - chr2_2:68624744-68624763 Msa0267170:intron 50.0%
! GAGATGGTTTTTCTCGAGGC+GGG - chr2_2:68624745-68624764 Msa0267170:intron 50.0%
! TTTTTCTCGAGGCGGGAGAA+GGG - chr2_2:68624752-68624771 Msa0267170:intron 50.0%
AAGGGGAATGCTAACCGAGA+AGG - chr2_2:68624770-68624789 Msa0267170:intron 50.0%
AACCGAGAAGGCGGTTATTG+AGG - chr2_2:68624782-68624801 Msa0267170:intron 50.0%
TGCCTCAATAACCGCCTTCT+CGG + chr2_2:68624787-68624806 None:intergenic 50.0%
GGTTATTGAGGCAGTGGAGT+TGG - chr2_2:68624794-68624813 Msa0267170:intron 50.0%
TATTGAGGCAGTGGAGTTGG+CGG - chr2_2:68624797-68624816 Msa0267170:intron 50.0%
CCTGAAGCATGCGCCAATTA+GGG + chr2_2:68625028-68625047 None:intergenic 50.0%
ACCTGAAGCATGCGCCAATT+AGG + chr2_2:68625029-68625048 None:intergenic 50.0%
CTTCACACACTTGTCTCGGT+TGG - chr2_2:68625559-68625578 Msa0267170:intron 50.0%
AGCTGAGATGGTCAGAGGAT+TGG + chr2_2:68625784-68625803 None:intergenic 50.0%
CAACTTGCCAAGGATTGACG+CGG + chr2_2:68626850-68626869 None:intergenic 50.0%
TAGATGCACTGCAGGATTGC+GGG + chr2_2:68626882-68626901 None:intergenic 50.0%
GTAGATGCACTGCAGGATTG+CGG + chr2_2:68626883-68626902 None:intergenic 50.0%
! CTATTTTCCAGCAGGCATGC+AGG - chr2_2:68627033-68627052 Msa0267170:CDS 50.0%
GGCGACAAGTCGTTAGATGT+GGG + chr2_2:68627154-68627173 None:intergenic 50.0%
TGGCGACAAGTCGTTAGATG+TGG + chr2_2:68627155-68627174 None:intergenic 50.0%
CACCCTTGGTACCAGAATCA+TGG + chr2_2:68627306-68627325 None:intergenic 50.0%
TGGAACGTTCACCGATGGAC+CGG + chr2_2:68624088-68624107 None:intergenic 55.0%
AGGATCGGCCAGGAGATCAA+CGG + chr2_2:68624235-68624254 None:intergenic 55.0%
GACCTCATCGGTGTGAGGAT+CGG + chr2_2:68624250-68624269 None:intergenic 55.0%
! GTTTTTCTCGAGGCGGGAGA+AGG - chr2_2:68624751-68624770 Msa0267170:intron 55.0%
! TTTTCTCGAGGCGGGAGAAG+GGG - chr2_2:68624753-68624772 Msa0267170:intron 55.0%
GGGAATGCTAACCGAGAAGG+CGG - chr2_2:68624773-68624792 Msa0267170:intron 55.0%
GAAGGCGGTTATTGAGGCAG+TGG - chr2_2:68624788-68624807 Msa0267170:intron 55.0%
CATGCGCCAATTAGGGACAC+TGG + chr2_2:68625021-68625040 None:intergenic 55.0%
CCCTAATTGGCGCATGCTTC+AGG - chr2_2:68625025-68625044 Msa0267170:intron 55.0%
GGTTGAGCTGAGATGGTCAG+AGG + chr2_2:68625789-68625808 None:intergenic 55.0%
! TGAGGAGCAAGAAGGGTGCA+TGG + chr2_2:68626499-68626518 None:intergenic 55.0%
TTGGATTTCACGCGGCGTGC+CGG + chr2_2:68624048-68624067 None:intergenic 60.0%
! GCAGACATGTGCCGGTCCAT+CGG - chr2_2:68624074-68624093 Msa0267170:CDS 60.0%
TTGAGCGTTAGGGGAAGGGC+AGG + chr2_2:68624160-68624179 None:intergenic 60.0%
CATCACGGCCGTTGATCTCC+TGG - chr2_2:68624224-68624243 Msa0267170:CDS 60.0%
GCGAAGACCTCATCGGTGTG+AGG + chr2_2:68624255-68624274 None:intergenic 60.0%
GACAGACATGGTGGCCGGTG+GGG + chr2_2:68624022-68624041 None:intergenic 65.0%
GGACAGACATGGTGGCCGGT+GGG + chr2_2:68624023-68624042 None:intergenic 65.0%
GGAGGGACAGACATGGTGGC+CGG + chr2_2:68624027-68624046 None:intergenic 65.0%
GGCCACCATGTCTGTCCCTC+CGG - chr2_2:68624026-68624045 Msa0267170:CDS 65.0%
TGCCGGAGGGACAGACATGG+TGG + chr2_2:68624031-68624050 None:intergenic 65.0%
ATTTCACGCGGCGTGCCGGA+GGG + chr2_2:68624044-68624063 None:intergenic 65.0%
CATCGGTGTGAGGATCGGCC+AGG + chr2_2:68624245-68624264 None:intergenic 65.0%
GGCCGATCCTCACACCGATG+AGG - chr2_2:68624245-68624264 Msa0267170:CDS 65.0%
CTGACTCCCTGCATGCCTGC+TGG + chr2_2:68627043-68627062 None:intergenic 65.0%
CAGCAGGCATGCAGGGAGTC+AGG - chr2_2:68627041-68627060 Msa0267170:CDS 65.0%
GGGACAGACATGGTGGCCGG+TGG + chr2_2:68624024-68624043 None:intergenic 70.0%
GCGTGCCGGAGGGACAGACA+TGG + chr2_2:68624034-68624053 None:intergenic 70.0%
GATTTCACGCGGCGTGCCGG+AGG + chr2_2:68624045-68624064 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 68624012 68627357 68624012 ID=Msa0267170;Name=Msa0267170
chr2_2 mRNA 68624012 68627357 68624012 ID=Msa0267170-mRNA-1;Parent=Msa0267170;Name=Msa0267170-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|0|0|1|1|1|2|0|527
chr2_2 exon 68626323 68627357 68626323 ID=Msa0267170-mRNA-1:exon:21772;Parent=Msa0267170-mRNA-1
chr2_2 exon 68624012 68624560 68624012 ID=Msa0267170-mRNA-1:exon:21771;Parent=Msa0267170-mRNA-1
chr2_2 CDS 68626323 68627357 68626323 ID=Msa0267170-mRNA-1:cds;Parent=Msa0267170-mRNA-1
chr2_2 CDS 68624012 68624560 68624012 ID=Msa0267170-mRNA-1:cds;Parent=Msa0267170-mRNA-1
Gene Sequence

>Msa0267170

ATGTCTTCCCCACCGGCCACCATGTCTGTCCCTCCGGCACGCCGCGTGAAATCCAAAATCTGGCAGACATGTGCCGGTCCATCGGTGAACGTTCCAAAGGTACGTTCCAAAGTTTATTACTTCCCACATGGTCACTTGGAACATGCCTGCCCTTCCCCTAACGCTCAAACAATCACCGTTATTGATGGTTACCGTCCTTCTTTTCCTTGTATCATCACGGCCGTTGATCTCCTGGCCGATCCTCACACCGATGAGGTCTTCGCCAAATTGCTACTCAGTCCGGTAACTGAAGGACAAGAACTTCCTGAGGTTGTTGATAAAGAAGAAGACGGTGATAAGGTTGTTTCCTTCGTAAAGACTCTTACAAAATCTGATTCTAATAACGGCGGTGGATTCTCTGTACCTCGCCTTTGTGCTGACTTGATCTTCCCCAAGCTTGACTTAAATTCTCCTTTTCCGTCTCAGGAACTCTCTGTTACTGATGTTCATGACGGAGTTTGGAAGTTTGCTCATGTCTATCGAGGGAGACCCAAAAGACACCTTTTTACTACTGGTTGGACTCCGTTTGTGAACACGAAAAAACTCGTTGCTGGTGATTCTATTGTTTTCATGAAAAACACTGCCGGAGATATAGTTGTTGGAATCCGCAGAAACATGAAGTTTGCTGCAGCTGAAGCAAAAGCAGTGAATAATAAGAAGGAGGAGGGTAAAGAGAATGGTTTGGAGGTTAAGGGAGATGGTTTTTCTCGAGGCGGGAGAAGGGGAATGCTAACCGAGAAGGCGGTTATTGAGGCAGTGGAGTTGGCGGAAAAGAATATGGCATTTGAAGTTATATATTACCCTAGGGCTAATTGGTGCAATTTTGTAGTTGATTCAAATGTTGTGGATGATGCTATGAAGATTGGTTGGGCATCTGGAATGAGAGTGAAGCTTCCTTTGAAGATTGATGAATCTTCAAATTCGAAGATGACATTTTTTCAGCCTCAAGGAACAATCTCTAATGTGTCCAGTGTCCCTAATTGGCGCATGCTTCAGGTTAACTGGGATGAACTTGAAATCTCGCAAAATCAAAACCGCGTCAATCCTTGGCAAGTTGAACTTATTTCCCGCAATCCTGCAGTGCATCTACCATTTCTCTCAACAAAAAAGCCTAGACTAGTTCAGGATTCTACCTTGTTTAGTGATAACAAGGGTGATCCCTTCATTCCTATGATAGAGTTCCCTCAGAGATCTTTGAACCAAACATTGTTGAATTGGGGCTATTTTCCAGCAGGCATGCAGGGAGTCAGGCATGATCATTTATCTCTATCAGGTTTTTCCAACTCTTTGAATGATAATAGTTATGGTTTCTTTGTGAACAATCTAAACATTGACAGTCCCACATCTAACGACTTGTCGCCAAATAACCTTAGTAGTTTGAACACATTCGGGACAGATTTTTTTGAGACTCACAACAACAACACAAAAGGAGTTGGTTCTGGTTCAATTAAGTTATTTGGTAAGATCATACAGACCGTTGAAAGTGATGTCCATGATTCTGGTACCAAGGGTGAAGATTCCTCTAAAGGATCCAATGAAATTTGA
Protein sequence

>Msa0267170

MSSPPATMSVPPARRVKSKIWQTCAGPSVNVPKVRSKVYYFPHGHLEHACPSPNAQTITVIDGYRPSFPCIITAVDLLADPHTDEVFAKLLLSPVTEGQELPEVVDKEEDGDKVVSFVKTLTKSDSNNGGGFSVPRLCADLIFPKLDLNSPFPSQELSVTDVHDGVWKFAHVYRGRPKRHLFTTGWTPFVNTKKLVAGDSIVFMKNTAGDIVVGIRRNMKFAAAEAKAVNNKKEEGKENGLEVKGDGFSRGGRRGMLTEKAVIEAVELAEKNMAFEVIYYPRANWCNFVVDSNVVDDAMKIGWASGMRVKLPLKIDESSNSKMTFFQPQGTISNVSSVPNWRMLQVNWDELEISQNQNRVNPWQVELISRNPAVHLPFLSTKKPRLVQDSTLFSDNKGDPFIPMIEFPQRSLNQTLLNWGYFPAGMQGVRHDHLSLSGFSNSLNDNSYGFFVNNLNIDSPTSNDLSPNNLSSLNTFGTDFFETHNNNTKGVGSGSIKLFGKIIQTVESDVHDSGTKGEDSSKGSNEI*