AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0267460

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0267460 MtrunA17_Chr4g0055451 66.667 162 24 3 9 142 66 225 4.64e-65 201
Msa0267460 MtrunA17_Chr4g0055431 64.815 162 27 3 9 142 72 231 1.34e-62 195
Msa0267460 MtrunA17_Chr8g0381821 59.877 162 35 3 9 142 65 224 1.72e-58 185
Msa0267460 MtrunA17_Chr1g0162301 57.396 169 42 3 9 149 85 251 2.71e-58 185
Msa0267460 MtrunA17_Chr4g0045371 59.259 162 36 3 9 142 84 243 1.19e-57 183
Msa0267460 MtrunA17_Chr8g0385281 59.259 162 36 3 9 142 66 225 5.64e-57 181
Msa0267460 MtrunA17_Chr5g0399911 58.025 162 38 3 9 142 80 239 1.83e-56 180
Msa0267460 MtrunA17_Chr3g0131061 58.025 162 38 3 9 142 65 224 2.91e-56 179
Msa0267460 MtrunA17_Chr2g0277501 58.025 162 38 3 9 142 65 224 3.16e-56 179
Msa0267460 MtrunA17_Chr8g0374571 55.030 169 46 3 9 149 84 250 4.50e-56 179
Msa0267460 MtrunA17_Chr5g0426521 53.143 175 52 3 9 155 65 237 2.52e-55 177
Msa0267460 MtrunA17_Chr5g0402661 52.663 169 50 3 9 149 79 245 7.16e-54 173
Msa0267460 MtrunA17_Chr3g0123791 56.790 162 40 3 9 142 66 225 1.25e-53 172
Msa0267460 MtrunA17_Chr8g0380661 45.679 162 55 4 9 142 65 221 5.48e-40 137
Msa0267460 MtrunA17_Chr8g0346081 36.774 155 62 6 9 133 82 230 2.73e-19 83.2
Msa0267460 MtrunA17_Chr8g0346071 34.132 167 71 7 9 145 82 239 3.21e-19 83.2
Msa0267460 MtrunA17_Chr6g0468751 34.211 152 68 3 9 132 84 231 3.38e-18 80.5
Msa0267460 MtrunA17_Chr6g0453681 34.211 152 68 3 9 132 84 231 3.67e-18 80.1
Msa0267460 MtrunA17_Chr2g0302431 33.108 148 64 5 9 127 85 226 1.32e-17 78.6
Msa0267460 MtrunA17_Chr7g0244521 33.735 166 74 7 9 145 77 235 1.40e-17 78.6
Msa0267460 MtrunA17_Chr5g0432491 34.667 150 67 4 9 129 88 235 2.86e-17 77.4
Msa0267460 MtrunA17_Chr3g0132671 33.333 162 72 7 9 142 114 267 3.58e-17 77.4
Msa0267460 MtrunA17_Chr4g0013131 34.839 155 66 7 9 133 77 226 4.07e-17 77.0
Msa0267460 MtrunA17_Chr2g0304111 36.879 141 58 4 9 120 88 226 6.19e-17 76.6
Msa0267460 MtrunA17_Chr1g0150321 32.515 163 74 6 9 142 78 233 2.47e-16 74.7
Msa0267460 MtrunA17_Chr4g0063881 33.987 153 62 6 9 129 76 221 4.66e-16 73.9
Msa0267460 MtrunA17_Chr5g0428031 36.620 142 55 5 9 120 74 210 5.04e-16 73.9
Msa0267460 MtrunA17_Chr4g0001331 33.742 163 72 6 9 142 85 240 5.58e-16 73.9
Msa0267460 MtrunA17_Chr3g0114511 33.333 150 68 5 9 130 97 242 6.31e-16 73.9
Msa0267460 MtrunA17_Chr3g0107051 31.609 174 86 6 9 152 86 256 1.01e-15 73.2
Msa0267460 MtrunA17_Chr1g0188031 32.624 141 64 3 9 120 87 225 4.07e-15 71.2
Msa0267460 MtrunA17_Chr2g0284011 30.723 166 79 6 9 145 77 235 5.46e-15 71.2
Msa0267460 MtrunA17_Chr4g0074111 32.877 146 56 6 9 120 83 220 6.96e-14 67.8
Msa0267460 MtrunA17_Chr4g0069301 33.108 148 64 5 9 127 91 232 1.81e-13 66.6
Msa0267460 MtrunA17_Chr4g0059751 33.962 159 70 5 11 142 163 313 2.20e-13 66.6
Msa0267460 MtrunA17_Chr2g0284031 32.624 141 63 5 9 120 96 233 5.23e-13 65.5
Msa0267460 MtrunA17_Chr6g0453671 28.289 152 77 3 9 132 84 231 5.62e-13 65.1
Msa0267460 MtrunA17_Chr8g0379091 32.877 146 62 6 9 123 91 231 5.91e-13 65.5
Msa0267460 MtrunA17_Chr2g0333821 31.126 151 72 5 9 130 79 226 9.94e-13 64.7
Msa0267460 MtrunA17_Chr1g0205421 28.659 164 79 5 9 142 77 232 3.56e-12 63.2
Msa0267460 MtrunA17_Chr3g0091281 36.232 138 57 6 9 118 77 211 5.24e-12 61.6
Msa0267460 MtrunA17_Chr8g0385341 34.459 148 66 5 19 138 106 250 5.76e-12 62.4
Msa0267460 MtrunA17_Chr4g0072831 31.538 130 59 4 9 120 54 171 9.38e-12 61.6
Msa0267460 MtrunA17_Chr3g0125381 30.189 159 77 5 11 142 19 170 1.40e-11 60.1
Msa0267460 MtrunA17_Chr3g0131181 33.784 148 61 5 19 135 105 246 1.53e-11 61.2
Msa0267460 MtrunA17_Chr1g0197721 31.613 155 68 6 17 135 124 276 2.80e-11 60.5
Msa0267460 MtrunA17_Chr7g0267771 31.875 160 78 5 4 135 149 305 3.40e-11 60.1
Msa0267460 MtrunA17_Chr8g0353621 31.126 151 67 5 19 142 85 225 4.56e-11 59.3
Msa0267460 MtrunA17_Chr8g0351491 29.452 146 70 4 11 129 83 222 5.98e-11 58.9
Msa0267460 MtrunA17_Chr4g0033371 33.071 127 56 4 19 118 148 272 9.68e-11 58.9
Msa0267460 MtrunA17_Chr7g0245031 33.333 135 59 4 11 118 215 345 9.76e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0267460 AT4G33950.2 58.025 162 38 3 9 142 82 241 2.77e-57 182
Msa0267460 AT4G33950.1 58.025 162 38 3 9 142 82 241 4.32e-57 182
Msa0267460 AT5G66880.1 55.621 169 45 3 9 149 83 249 7.63e-57 181
Msa0267460 AT5G66880.2 55.621 169 45 3 9 149 83 249 7.63e-57 181
Msa0267460 AT1G10940.1 58.025 162 38 3 9 142 65 224 1.47e-56 181
Msa0267460 AT5G08590.1 59.259 162 36 3 9 142 65 224 1.79e-56 180
Msa0267460 AT3G50500.1 58.025 162 38 3 9 142 84 243 2.73e-56 180
Msa0267460 AT3G50500.2 58.084 167 37 4 9 142 84 250 7.53e-56 179
Msa0267460 AT1G10940.2 55.882 170 37 3 9 142 65 232 1.14e-55 179
Msa0267460 AT5G63650.1 58.025 162 38 3 9 142 65 224 2.66e-55 177
Msa0267460 AT1G60940.1 56.790 162 40 3 9 142 65 224 3.94e-55 177
Msa0267460 AT1G60940.2 56.790 162 40 3 9 142 65 224 3.94e-55 177
Msa0267460 AT4G40010.1 58.025 162 38 3 9 142 65 224 6.23e-55 176
Msa0267460 AT2G23030.1 55.556 162 42 3 9 142 65 224 1.27e-54 175
Msa0267460 AT1G78290.3 56.173 162 41 3 9 142 65 224 1.80e-54 175
Msa0267460 AT1G78290.2 56.173 162 41 3 9 142 65 224 1.80e-54 175
Msa0267460 AT5G08590.2 58.000 150 33 3 21 142 1 148 5.67e-50 161
Msa0267460 AT5G35410.2 34.524 168 79 4 9 148 76 240 2.06e-18 80.9
Msa0267460 AT5G35410.1 34.524 168 79 4 9 148 76 240 2.07e-18 81.3
Msa0267460 AT1G01140.2 35.664 143 61 4 8 121 83 223 4.13e-18 80.5
Msa0267460 AT1G01140.1 35.664 143 61 4 8 121 83 223 4.20e-18 80.5
Msa0267460 AT1G01140.3 35.664 143 61 4 8 121 83 223 4.32e-18 80.5
Msa0267460 AT3G01090.2 34.194 155 64 4 9 132 107 254 6.59e-18 80.1
Msa0267460 AT3G01090.3 34.194 155 64 4 9 132 84 231 7.42e-18 79.7
Msa0267460 AT3G01090.1 34.194 155 64 4 9 132 84 231 7.42e-18 79.7
Msa0267460 AT5G39440.1 32.298 161 74 5 9 142 84 236 1.49e-17 79.0
Msa0267460 AT4G24400.2 36.429 140 58 4 9 120 74 210 1.60e-17 78.6
Msa0267460 AT1G30270.2 34.752 141 61 4 9 120 96 234 1.89e-17 78.6
Msa0267460 AT1G30270.1 34.752 141 61 4 9 120 96 234 1.97e-17 78.6
Msa0267460 AT4G24400.1 36.429 140 58 4 9 120 74 210 2.15e-17 78.2
Msa0267460 AT3G29160.3 33.548 155 65 4 9 132 85 232 4.85e-17 77.0
Msa0267460 AT5G58380.1 33.117 154 70 6 9 133 77 226 6.61e-17 77.0
Msa0267460 AT2G26980.2 35.664 143 57 5 9 120 79 217 8.47e-17 75.5
Msa0267460 AT3G29160.1 33.548 155 65 4 9 132 85 232 9.54e-17 76.6
Msa0267460 AT3G29160.2 33.548 155 65 4 9 132 85 232 9.54e-17 76.6
Msa0267460 AT1G30270.3 35.036 137 58 4 13 120 3 137 1.17e-16 75.9
Msa0267460 AT2G26980.3 35.664 143 57 5 9 120 79 217 2.91e-16 75.1
Msa0267460 AT2G26980.1 35.664 143 57 5 9 120 79 217 2.91e-16 75.1
Msa0267460 AT2G26980.4 35.664 143 57 5 9 120 89 227 3.01e-16 75.1
Msa0267460 AT2G26980.5 35.664 143 57 5 9 120 79 217 3.39e-16 74.7
Msa0267460 AT3G23000.1 32.927 164 72 5 9 142 91 246 4.45e-16 74.7
Msa0267460 AT5G21222.1 33.333 141 63 4 9 120 78 216 6.98e-16 74.3
Msa0267460 AT5G21222.2 33.333 141 63 4 9 120 78 216 6.98e-16 74.3
Msa0267460 AT5G21222.4 33.333 141 63 4 9 120 78 216 6.98e-16 74.3
Msa0267460 AT5G21222.3 33.333 141 63 4 9 120 78 216 6.98e-16 74.3
Msa0267460 AT5G45820.1 33.775 151 66 6 9 129 77 223 1.03e-14 70.9
Msa0267460 AT5G01810.3 33.537 164 71 8 9 142 77 232 1.98e-14 69.7
Msa0267460 AT5G01810.1 33.537 164 71 8 9 142 77 232 2.52e-14 69.7
Msa0267460 AT5G01810.2 33.537 164 71 8 9 142 77 232 2.52e-14 69.7
Msa0267460 AT5G10930.1 32.667 150 69 5 9 129 78 224 3.68e-14 68.9
Msa0267460 AT3G17510.1 31.757 148 66 5 9 127 85 226 7.30e-14 68.2
Msa0267460 AT5G04510.3 34.356 163 65 7 11 135 111 269 8.40e-14 67.4
Msa0267460 AT5G25110.1 30.667 150 72 5 9 129 108 254 1.37e-13 67.4
Msa0267460 AT5G45810.1 33.333 141 62 5 9 120 93 230 1.54e-13 67.4
Msa0267460 AT5G04510.1 34.146 164 66 7 10 135 110 269 1.59e-13 67.4
Msa0267460 AT5G04510.2 34.146 164 66 7 10 135 110 269 2.44e-13 66.6
Msa0267460 AT4G18700.1 32.624 141 63 5 9 120 91 228 4.89e-13 65.9
Msa0267460 AT3G10540.1 33.537 164 67 7 10 135 111 270 5.87e-13 65.9
Msa0267460 AT1G29230.1 31.788 151 59 6 3 118 133 274 9.02e-13 65.1
Msa0267460 AT4G30960.1 32.237 152 67 6 9 129 89 235 1.84e-12 64.3
Msa0267460 AT5G57630.1 32.143 140 62 5 9 120 77 211 5.74e-12 62.8
Msa0267460 AT1G35670.1 34.028 144 66 4 19 135 102 243 5.76e-12 62.8
Msa0267460 AT5G57630.2 32.143 140 62 5 9 120 77 211 6.13e-12 62.8
Msa0267460 AT5G07070.1 32.468 154 71 6 9 133 77 226 1.07e-11 62.0
Msa0267460 AT4G09570.1 34.722 144 65 4 19 135 101 242 1.09e-11 62.0
Msa0267460 AT2G45490.2 29.605 152 70 5 19 143 97 238 2.66e-11 60.1
Msa0267460 AT2G45490.1 29.605 152 70 5 19 143 97 238 3.58e-11 60.1
Msa0267460 AT1G49180.1 30.909 165 85 5 17 154 79 241 7.30e-11 59.7
Msa0267460 AT1G49180.2 30.909 165 85 5 17 154 79 241 7.40e-11 59.7

Find 41 sgRNAs with CRISPR-Local

Find 140 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGAAATTTCTATGATCTTC+TGG 0.176143 2_2:+69063161 None:intergenic
TCATCATAGAGATTTGAAAT+TGG 0.298226 2_2:-69063696 Msa0267460:CDS
GAAATTTCAGAAAGACTATT+GGG 0.308763 2_2:-69063148 Msa0267460:CDS
AGAAATTTCAGAAAGACTAT+TGG 0.330609 2_2:-69063149 Msa0267460:CDS
CGGCATAAATTTGTACATCA+TGG 0.373472 2_2:+69063002 None:intergenic
GGAGTTCCAACTGTCGCCTT+TGG 0.373657 2_2:+69063498 None:intergenic
TGATCTTCTGGTTCTTCAAA+TGG 0.389191 2_2:+69063173 None:intergenic
GAAATTGGAAAACACTCTCT+TGG 0.399280 2_2:-69063681 Msa0267460:CDS
ATTTCTGTTCTGCACTCTGC+AGG 0.405968 2_2:-69064051 Msa0267460:CDS
TGAAACACTGTTATGATTGT+CGG 0.421841 2_2:+69062982 None:intergenic
TTCTCATCTAGCCATTGTCT+TGG 0.448653 2_2:-69064015 Msa0267460:CDS
TTCATGAGGATAGGCACAAC+CGG 0.498161 2_2:-69063969 Msa0267460:CDS
GGCATAAATTTGTACATCAT+GGG 0.509669 2_2:+69063003 None:intergenic
TTGGAAAACACTCTCTTGGA+TGG 0.513601 2_2:-69063677 Msa0267460:CDS
CAAGATTTAAGCCGAGGGGA+TGG 0.525817 2_2:+69063650 None:intergenic
ATGTGAATTACTATTAACCT+TGG 0.541672 2_2:+69063613 None:intergenic
AAGACAATGGCTAGATGAGA+AGG 0.542672 2_2:+69064017 None:intergenic
TCCTTCATTCAGTGCTCCTG+AGG 0.551255 2_2:-69063478 Msa0267460:intron
GTCATAAAGTGTCACACCAC+AGG 0.555054 2_2:+69063214 None:intergenic
ACCTCAGGAGCACTGAATGA+AGG 0.560856 2_2:+69063477 None:intergenic
CAGTTCTCACTCTGATCCAA+AGG 0.569817 2_2:-69063514 Msa0267460:CDS
ACACTTTATGACATGCTAGT+TGG 0.570446 2_2:-69063203 Msa0267460:CDS
CTCGTCTTCACTAAATCGAC+CGG 0.572882 2_2:+69063950 None:intergenic
TTGCAACTTCGATTACTCCA+AGG 0.574622 2_2:-69063630 Msa0267460:CDS
TCTGATCCAAAGGCGACAGT+TGG 0.577370 2_2:-69063504 Msa0267460:CDS
AGACAATGGCTAGATGAGAA+GGG 0.591203 2_2:+69064018 None:intergenic
ATTGCAGATGTGTGGTCCTG+TGG 0.607065 2_2:-69063230 Msa0267460:CDS
AAGTTGCAAGATTTAAGCCG+AGG 0.609007 2_2:+69063644 None:intergenic
CGGTCGATTTAGTGAAGACG+AGG 0.612886 2_2:-69063949 Msa0267460:intron
GGTGAACTCTTCATGAGGAT+AGG 0.616301 2_2:-69063978 Msa0267460:CDS
AGTTGCAAGATTTAAGCCGA+GGG 0.618700 2_2:+69063645 None:intergenic
AAATTTGTACATCATGGGAA+AGG 0.618936 2_2:+69063008 None:intergenic
TTGTATAGATTGCAGATGTG+TGG 0.620311 2_2:-69063238 Msa0267460:intron
TGGATGGAAATCCATCCCCT+CGG 0.620502 2_2:-69063661 Msa0267460:CDS
ATTGTCTTGGAGTATGCTGA+TGG 0.625672 2_2:-69064002 Msa0267460:CDS
GACAATGGCTAGATGAGAAG+GGG 0.626872 2_2:+69064019 None:intergenic
GTCTTGGAGTATGCTGATGG+TGG 0.656453 2_2:-69063999 Msa0267460:CDS
ATCAGCATACTCCAAGACAA+TGG 0.671309 2_2:+69064004 None:intergenic
AAATTTCAGAAAGACTATTG+GGG 0.682464 2_2:-69063147 Msa0267460:intron
ATGGTGGTGAACTCTTCATG+AGG 0.713505 2_2:-69063983 Msa0267460:CDS
GTTGCAAGATTTAAGCCGAG+GGG 0.717502 2_2:+69063646 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATACGATTTTTTTTTAAA+AGG + chr2_2:69063149-69063168 None:intergenic 10.0%
!!! ATATACGATTTTTTTTTAAA+AGG + chr2_2:69063149-69063168 None:intergenic 10.0%
!! AATATGATATATCTTCAAAA+TGG + chr2_2:69063455-69063474 None:intergenic 15.0%
!! AATATGATATATCTTCAAAA+TGG + chr2_2:69063455-69063474 None:intergenic 15.0%
! TTATTCCAAAAGCTAATATC+TGG - chr2_2:69063223-69063242 Msa0267460:CDS 25.0%
!! TCATCATAGAGATTTGAAAT+TGG - chr2_2:69063338-69063357 Msa0267460:intron 25.0%
! ATGTGAATTACTATTAACCT+TGG + chr2_2:69063424-69063443 None:intergenic 25.0%
! AAAATGATTGATAAAATCCC+AGG + chr2_2:69063614-69063633 None:intergenic 25.0%
!! TTTTATCAATCATTTTCTCG+AGG - chr2_2:69063618-69063637 Msa0267460:intron 25.0%
!! TTTATCAATCATTTTCTCGA+GGG - chr2_2:69063619-69063638 Msa0267460:intron 25.0%
!!! AATTTGAAACGTGGTTTTTA+AGG + chr2_2:69063645-69063664 None:intergenic 25.0%
!!! CAAATTCAAACCACTTTTTA+TGG - chr2_2:69063659-69063678 Msa0267460:CDS 25.0%
! AGAAATTTCAGAAAGACTAT+TGG - chr2_2:69063885-69063904 Msa0267460:intron 25.0%
! GAAATTTCAGAAAGACTATT+GGG - chr2_2:69063886-69063905 Msa0267460:intron 25.0%
! AAATTTCAGAAAGACTATTG+GGG - chr2_2:69063887-69063906 Msa0267460:intron 25.0%
! AAGAGATTATTCATAGTGAA+TGG + chr2_2:69063944-69063963 None:intergenic 25.0%
! TATGAATAATCTCTTATAGC+AGG - chr2_2:69063949-69063968 Msa0267460:intron 25.0%
! TTATTCCAAAAGCTAATATC+TGG - chr2_2:69063223-69063242 Msa0267460:CDS 25.0%
!! TCATCATAGAGATTTGAAAT+TGG - chr2_2:69063338-69063357 Msa0267460:intron 25.0%
! ATGTGAATTACTATTAACCT+TGG + chr2_2:69063424-69063443 None:intergenic 25.0%
! AAAATGATTGATAAAATCCC+AGG + chr2_2:69063614-69063633 None:intergenic 25.0%
!! TTTTATCAATCATTTTCTCG+AGG - chr2_2:69063618-69063637 Msa0267460:intron 25.0%
!! TTTATCAATCATTTTCTCGA+GGG - chr2_2:69063619-69063638 Msa0267460:intron 25.0%
!!! AATTTGAAACGTGGTTTTTA+AGG + chr2_2:69063645-69063664 None:intergenic 25.0%
!!! CAAATTCAAACCACTTTTTA+TGG - chr2_2:69063659-69063678 Msa0267460:CDS 25.0%
! AGAAATTTCAGAAAGACTAT+TGG - chr2_2:69063885-69063904 Msa0267460:intron 25.0%
! GAAATTTCAGAAAGACTATT+GGG - chr2_2:69063886-69063905 Msa0267460:intron 25.0%
! AAATTTCAGAAAGACTATTG+GGG - chr2_2:69063887-69063906 Msa0267460:intron 25.0%
! AAGAGATTATTCATAGTGAA+TGG + chr2_2:69063944-69063963 None:intergenic 25.0%
! TATGAATAATCTCTTATAGC+AGG - chr2_2:69063949-69063968 Msa0267460:intron 25.0%
ATCTTGCATGTAGCAAATAT+AGG + chr2_2:69063201-69063220 None:intergenic 30.0%
!!! TAACACCAGATATTAGCTTT+TGG + chr2_2:69063231-69063250 None:intergenic 30.0%
TGTTAGCTACTATCATTCAA+TGG - chr2_2:69063246-69063265 Msa0267460:intron 30.0%
CATGTCTTATATTACGCTTT+AGG - chr2_2:69063311-69063330 Msa0267460:intron 30.0%
CTGTTAATAGCCATAAAAAG+TGG + chr2_2:69063672-69063691 None:intergenic 30.0%
TTTATGGCTATTAACAGACA+TGG - chr2_2:69063675-69063694 Msa0267460:CDS 30.0%
!! GTTGATTCAGTTAGTGTTTT+TGG - chr2_2:69063767-69063786 Msa0267460:intron 30.0%
CTGAAATTTCTATGATCTTC+TGG + chr2_2:69063876-69063895 None:intergenic 30.0%
AAATTTGTACATCATGGGAA+AGG + chr2_2:69064029-69064048 None:intergenic 30.0%
GGCATAAATTTGTACATCAT+GGG + chr2_2:69064034-69064053 None:intergenic 30.0%
TGAAACACTGTTATGATTGT+CGG + chr2_2:69064055-69064074 None:intergenic 30.0%
ATCTTGCATGTAGCAAATAT+AGG + chr2_2:69063201-69063220 None:intergenic 30.0%
!!! TAACACCAGATATTAGCTTT+TGG + chr2_2:69063231-69063250 None:intergenic 30.0%
TGTTAGCTACTATCATTCAA+TGG - chr2_2:69063246-69063265 Msa0267460:intron 30.0%
CATGTCTTATATTACGCTTT+AGG - chr2_2:69063311-69063330 Msa0267460:intron 30.0%
CTGTTAATAGCCATAAAAAG+TGG + chr2_2:69063672-69063691 None:intergenic 30.0%
TTTATGGCTATTAACAGACA+TGG - chr2_2:69063675-69063694 Msa0267460:CDS 30.0%
!! GTTGATTCAGTTAGTGTTTT+TGG - chr2_2:69063767-69063786 Msa0267460:intron 30.0%
CTGAAATTTCTATGATCTTC+TGG + chr2_2:69063876-69063895 None:intergenic 30.0%
AAATTTGTACATCATGGGAA+AGG + chr2_2:69064029-69064048 None:intergenic 30.0%
GGCATAAATTTGTACATCAT+GGG + chr2_2:69064034-69064053 None:intergenic 30.0%
TGAAACACTGTTATGATTGT+CGG + chr2_2:69064055-69064074 None:intergenic 30.0%
CACAAATAATTATGAGCGCA+TGG + chr2_2:69063275-69063294 None:intergenic 35.0%
GAAATTGGAAAACACTCTCT+TGG - chr2_2:69063353-69063372 Msa0267460:intron 35.0%
! CTGTTATGAAAGGAGTACAA+CGG - chr2_2:69063581-69063600 Msa0267460:intron 35.0%
! AGTGGTTTGAATTTGAAACG+TGG + chr2_2:69063654-69063673 None:intergenic 35.0%
TTGTATAGATTGCAGATGTG+TGG - chr2_2:69063796-69063815 Msa0267460:intron 35.0%
ACACTTTATGACATGCTAGT+TGG - chr2_2:69063831-69063850 Msa0267460:intron 35.0%
!! TGATCTTCTGGTTCTTCAAA+TGG + chr2_2:69063864-69063883 None:intergenic 35.0%
!! GTGAGCTCTAATTTTACTTC+AGG - chr2_2:69063909-69063928 Msa0267460:intron 35.0%
CGGCATAAATTTGTACATCA+TGG + chr2_2:69064035-69064054 None:intergenic 35.0%
CACAAATAATTATGAGCGCA+TGG + chr2_2:69063275-69063294 None:intergenic 35.0%
GAAATTGGAAAACACTCTCT+TGG - chr2_2:69063353-69063372 Msa0267460:intron 35.0%
! CTGTTATGAAAGGAGTACAA+CGG - chr2_2:69063581-69063600 Msa0267460:intron 35.0%
! AGTGGTTTGAATTTGAAACG+TGG + chr2_2:69063654-69063673 None:intergenic 35.0%
TTGTATAGATTGCAGATGTG+TGG - chr2_2:69063796-69063815 Msa0267460:intron 35.0%
ACACTTTATGACATGCTAGT+TGG - chr2_2:69063831-69063850 Msa0267460:intron 35.0%
!! TGATCTTCTGGTTCTTCAAA+TGG + chr2_2:69063864-69063883 None:intergenic 35.0%
!! GTGAGCTCTAATTTTACTTC+AGG - chr2_2:69063909-69063928 Msa0267460:intron 35.0%
CGGCATAAATTTGTACATCA+TGG + chr2_2:69064035-69064054 None:intergenic 35.0%
AGACAATGGCTAGATGAGAA+GGG + chr2_2:69063019-69063038 None:intergenic 40.0%
AAGACAATGGCTAGATGAGA+AGG + chr2_2:69063020-69063039 None:intergenic 40.0%
TTCTCATCTAGCCATTGTCT+TGG - chr2_2:69063019-69063038 Msa0267460:CDS 40.0%
ATCAGCATACTCCAAGACAA+TGG + chr2_2:69063033-69063052 None:intergenic 40.0%
ATTGTCTTGGAGTATGCTGA+TGG - chr2_2:69063032-69063051 Msa0267460:CDS 40.0%
TTGGAAAACACTCTCTTGGA+TGG - chr2_2:69063357-69063376 Msa0267460:intron 40.0%
AGTTGCAAGATTTAAGCCGA+GGG + chr2_2:69063392-69063411 None:intergenic 40.0%
AAGTTGCAAGATTTAAGCCG+AGG + chr2_2:69063393-69063412 None:intergenic 40.0%
TTGCAACTTCGATTACTCCA+AGG - chr2_2:69063404-69063423 Msa0267460:intron 40.0%
TCCTGAGGTTCTGTTATGAA+AGG - chr2_2:69063571-69063590 Msa0267460:intron 40.0%
TCCTTTCATAACAGAACCTC+AGG + chr2_2:69063575-69063594 None:intergenic 40.0%
! ATGAAAGGAGTACAACGGAA+AGG - chr2_2:69063586-69063605 Msa0267460:intron 40.0%
!! AACAGCTATTTTCCTCTACC+AGG + chr2_2:69063999-69064018 None:intergenic 40.0%
AGACAATGGCTAGATGAGAA+GGG + chr2_2:69063019-69063038 None:intergenic 40.0%
AAGACAATGGCTAGATGAGA+AGG + chr2_2:69063020-69063039 None:intergenic 40.0%
TTCTCATCTAGCCATTGTCT+TGG - chr2_2:69063019-69063038 Msa0267460:CDS 40.0%
ATCAGCATACTCCAAGACAA+TGG + chr2_2:69063033-69063052 None:intergenic 40.0%
ATTGTCTTGGAGTATGCTGA+TGG - chr2_2:69063032-69063051 Msa0267460:CDS 40.0%
TTGGAAAACACTCTCTTGGA+TGG - chr2_2:69063357-69063376 Msa0267460:intron 40.0%
AGTTGCAAGATTTAAGCCGA+GGG + chr2_2:69063392-69063411 None:intergenic 40.0%
AAGTTGCAAGATTTAAGCCG+AGG + chr2_2:69063393-69063412 None:intergenic 40.0%
TTGCAACTTCGATTACTCCA+AGG - chr2_2:69063404-69063423 Msa0267460:intron 40.0%
TCCTGAGGTTCTGTTATGAA+AGG - chr2_2:69063571-69063590 Msa0267460:intron 40.0%
TCCTTTCATAACAGAACCTC+AGG + chr2_2:69063575-69063594 None:intergenic 40.0%
! ATGAAAGGAGTACAACGGAA+AGG - chr2_2:69063586-69063605 Msa0267460:intron 40.0%
!! AACAGCTATTTTCCTCTACC+AGG + chr2_2:69063999-69064018 None:intergenic 40.0%
ATTTCTGTTCTGCACTCTGC+AGG - chr2_2:69062983-69063002 Msa0267460:CDS 45.0%
GACAATGGCTAGATGAGAAG+GGG + chr2_2:69063018-69063037 None:intergenic 45.0%
! ATGGTGGTGAACTCTTCATG+AGG - chr2_2:69063051-69063070 Msa0267460:intron 45.0%
GGTGAACTCTTCATGAGGAT+AGG - chr2_2:69063056-69063075 Msa0267460:intron 45.0%
TTCATGAGGATAGGCACAAC+CGG - chr2_2:69063065-69063084 Msa0267460:intron 45.0%
CTCGTCTTCACTAAATCGAC+CGG + chr2_2:69063087-69063106 None:intergenic 45.0%
GTTGCAAGATTTAAGCCGAG+GGG + chr2_2:69063391-69063410 None:intergenic 45.0%
CAGTTCTCACTCTGATCCAA+AGG - chr2_2:69063520-69063539 Msa0267460:CDS 45.0%
!! AGTACAACGGAAAGGTACCT+GGG - chr2_2:69063594-69063613 Msa0267460:intron 45.0%
! GTCATAAAGTGTCACACCAC+AGG + chr2_2:69063823-69063842 None:intergenic 45.0%
GAACTCTGATTCTCTATGCC+TGG - chr2_2:69063978-69063997 Msa0267460:CDS 45.0%
TGATTCTCTATGCCTGGTAG+AGG - chr2_2:69063984-69064003 Msa0267460:CDS 45.0%
ATTTCTGTTCTGCACTCTGC+AGG - chr2_2:69062983-69063002 Msa0267460:CDS 45.0%
GACAATGGCTAGATGAGAAG+GGG + chr2_2:69063018-69063037 None:intergenic 45.0%
! ATGGTGGTGAACTCTTCATG+AGG - chr2_2:69063051-69063070 Msa0267460:intron 45.0%
GGTGAACTCTTCATGAGGAT+AGG - chr2_2:69063056-69063075 Msa0267460:intron 45.0%
TTCATGAGGATAGGCACAAC+CGG - chr2_2:69063065-69063084 Msa0267460:intron 45.0%
CTCGTCTTCACTAAATCGAC+CGG + chr2_2:69063087-69063106 None:intergenic 45.0%
GTTGCAAGATTTAAGCCGAG+GGG + chr2_2:69063391-69063410 None:intergenic 45.0%
CAGTTCTCACTCTGATCCAA+AGG - chr2_2:69063520-69063539 Msa0267460:CDS 45.0%
!! AGTACAACGGAAAGGTACCT+GGG - chr2_2:69063594-69063613 Msa0267460:intron 45.0%
! GTCATAAAGTGTCACACCAC+AGG + chr2_2:69063823-69063842 None:intergenic 45.0%
GAACTCTGATTCTCTATGCC+TGG - chr2_2:69063978-69063997 Msa0267460:CDS 45.0%
TGATTCTCTATGCCTGGTAG+AGG - chr2_2:69063984-69064003 Msa0267460:CDS 45.0%
GTCTTGGAGTATGCTGATGG+TGG - chr2_2:69063035-69063054 Msa0267460:intron 50.0%
! CGGTCGATTTAGTGAAGACG+AGG - chr2_2:69063085-69063104 Msa0267460:intron 50.0%
TGGATGGAAATCCATCCCCT+CGG - chr2_2:69063373-69063392 Msa0267460:intron 50.0%
CAAGATTTAAGCCGAGGGGA+TGG + chr2_2:69063387-69063406 None:intergenic 50.0%
TCTGATCCAAAGGCGACAGT+TGG - chr2_2:69063530-69063549 Msa0267460:intron 50.0%
TCCTTCATTCAGTGCTCCTG+AGG - chr2_2:69063556-69063575 Msa0267460:intron 50.0%
!! ACCTCAGGAGCACTGAATGA+AGG + chr2_2:69063560-69063579 None:intergenic 50.0%
!! GAGTACAACGGAAAGGTACC+TGG - chr2_2:69063593-69063612 Msa0267460:intron 50.0%
ATTGCAGATGTGTGGTCCTG+TGG - chr2_2:69063804-69063823 Msa0267460:intron 50.0%
GTCTTGGAGTATGCTGATGG+TGG - chr2_2:69063035-69063054 Msa0267460:intron 50.0%
! CGGTCGATTTAGTGAAGACG+AGG - chr2_2:69063085-69063104 Msa0267460:intron 50.0%
TGGATGGAAATCCATCCCCT+CGG - chr2_2:69063373-69063392 Msa0267460:intron 50.0%
CAAGATTTAAGCCGAGGGGA+TGG + chr2_2:69063387-69063406 None:intergenic 50.0%
TCTGATCCAAAGGCGACAGT+TGG - chr2_2:69063530-69063549 Msa0267460:intron 50.0%
TCCTTCATTCAGTGCTCCTG+AGG - chr2_2:69063556-69063575 Msa0267460:intron 50.0%
!! ACCTCAGGAGCACTGAATGA+AGG + chr2_2:69063560-69063579 None:intergenic 50.0%
!! GAGTACAACGGAAAGGTACC+TGG - chr2_2:69063593-69063612 Msa0267460:intron 50.0%
ATTGCAGATGTGTGGTCCTG+TGG - chr2_2:69063804-69063823 Msa0267460:intron 50.0%
GGAGTTCCAACTGTCGCCTT+TGG + chr2_2:69063539-69063558 None:intergenic 55.0%
GGAGTTCCAACTGTCGCCTT+TGG + chr2_2:69063539-69063558 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 69062978 69064078 69062978 ID=Msa0267460;Name=Msa0267460
chr2_2 mRNA 69062978 69064078 69062978 ID=Msa0267460-mRNA-1;Parent=Msa0267460;Name=Msa0267460-mRNA-1;_AED=0.18;_eAED=0.18;_QI=0|0|0|1|0.75|0.6|5|0|157
chr2_2 exon 69063950 69064078 69063950 ID=Msa0267460-mRNA-1:exon:21980;Parent=Msa0267460-mRNA-1
chr2_2 exon 69063624 69063723 69063624 ID=Msa0267460-mRNA-1:exon:21979;Parent=Msa0267460-mRNA-1
chr2_2 exon 69063479 69063540 69063479 ID=Msa0267460-mRNA-1:exon:21978;Parent=Msa0267460-mRNA-1
chr2_2 exon 69063148 69063252 69063148 ID=Msa0267460-mRNA-1:exon:21977;Parent=Msa0267460-mRNA-1
chr2_2 exon 69062978 69063052 69062978 ID=Msa0267460-mRNA-1:exon:21976;Parent=Msa0267460-mRNA-1
chr2_2 CDS 69063950 69064078 69063950 ID=Msa0267460-mRNA-1:cds;Parent=Msa0267460-mRNA-1
chr2_2 CDS 69063624 69063723 69063624 ID=Msa0267460-mRNA-1:cds;Parent=Msa0267460-mRNA-1
chr2_2 CDS 69063479 69063540 69063479 ID=Msa0267460-mRNA-1:cds;Parent=Msa0267460-mRNA-1
chr2_2 CDS 69063148 69063252 69063148 ID=Msa0267460-mRNA-1:cds;Parent=Msa0267460-mRNA-1
chr2_2 CDS 69062978 69063052 69062978 ID=Msa0267460-mRNA-1:cds;Parent=Msa0267460-mRNA-1
Gene Sequence

>Msa0267460

ATGAGATTTCTGTTCTGCACTCTGCAGGTCTTTCTTACCCCTTCTCATCTAGCCATTGTCTTGGAGTATGCTGATGGTGGTGAACTCTTCATGAGGATAGGCACAACCGGTCGATTTAGTGAAGACGAGGAAATTCATCATAGAGATTTGAAATTGGAAAACACTCTCTTGGATGGAAATCCATCCCCTCGGCTTAAATCTTGCAACTTCGATTACTCCAAGGTTAATATCTGCAGTTCTCACTCTGATCCAAAGGCGACAGTTGGAACTCCTTCATTCAGTGCTCCTGAGATTGCAGATGTGTGGTCCTGTGGTGTGACACTTTATGACATGCTAGTTGGTGCATATCCATTTGAAGAACCAGAAGATCATAGAAATTTCAGAAAGACTATTGGGAGGAAAATAGCTGTTCAATACTCCTTTCCCATGATGTACAAATTTATGCCGACAATCATAACAGTGTTTCAAACT
Protein sequence

>Msa0267460

MRFLFCTLQVFLTPSHLAIVLEYADGGELFMRIGTTGRFSEDEEIHHRDLKLENTLLDGNPSPRLKSCNFDYSKVNICSSHSDPKATVGTPSFSAPEIADVWSCGVTLYDMLVGAYPFEEPEDHRNFRKTIGRKIAVQYSFPMMYKFMPTIITVFQT