AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0267520

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0267520 MtrunA17_Chr4g0055451 66.447 152 21 3 9 132 66 215 1.07e-59 187
Msa0267520 MtrunA17_Chr4g0055431 64.474 152 24 3 9 132 72 221 1.87e-57 181
Msa0267520 MtrunA17_Chr8g0381821 60.927 151 29 3 9 131 65 213 1.18e-54 174
Msa0267520 MtrunA17_Chr4g0045371 59.868 152 31 3 9 132 84 233 6.24e-54 172
Msa0267520 MtrunA17_Chr1g0162301 59.603 151 31 3 9 131 85 233 9.75e-54 172
Msa0267520 MtrunA17_Chr5g0399911 58.940 151 32 3 9 131 80 228 7.46e-53 170
Msa0267520 MtrunA17_Chr8g0385281 59.603 151 31 3 9 131 66 214 1.51e-52 169
Msa0267520 MtrunA17_Chr3g0131061 58.940 151 32 3 9 131 65 213 6.26e-52 167
Msa0267520 MtrunA17_Chr2g0277501 58.278 151 33 3 9 131 65 213 1.72e-51 166
Msa0267520 MtrunA17_Chr8g0374571 56.954 151 35 3 9 131 84 232 3.56e-51 166
Msa0267520 MtrunA17_Chr5g0426521 57.616 151 34 3 9 131 65 213 1.24e-50 164
Msa0267520 MtrunA17_Chr3g0123791 57.616 151 34 3 9 131 66 214 6.20e-50 162
Msa0267520 MtrunA17_Chr5g0402661 54.305 151 39 3 9 131 79 227 7.79e-49 159
Msa0267520 MtrunA17_Chr8g0380661 46.053 152 49 4 9 132 65 211 9.89e-37 127
Msa0267520 MtrunA17_Chr8g0346081 35.526 152 63 5 8 129 81 227 5.17e-19 81.6
Msa0267520 MtrunA17_Chr8g0346071 35.417 144 58 5 8 121 81 219 6.28e-19 81.3
Msa0267520 MtrunA17_Chr6g0468751 34.211 152 68 3 9 132 84 231 2.18e-18 79.7
Msa0267520 MtrunA17_Chr6g0453681 34.211 152 68 3 9 132 84 231 2.33e-18 79.7
Msa0267520 MtrunA17_Chr5g0432491 34.667 150 67 4 9 129 88 235 7.20e-18 78.2
Msa0267520 MtrunA17_Chr2g0302431 33.108 148 64 5 9 127 85 226 7.89e-18 78.2
Msa0267520 MtrunA17_Chr7g0244521 34.667 150 66 5 9 129 77 223 2.71e-17 76.6
Msa0267520 MtrunA17_Chr4g0013131 34.437 151 65 6 9 129 77 223 2.86e-17 76.6
Msa0267520 MtrunA17_Chr2g0304111 36.879 141 58 4 9 120 88 226 3.88e-17 76.3
Msa0267520 MtrunA17_Chr3g0132671 33.557 149 67 5 9 129 114 258 5.98e-17 75.9
Msa0267520 MtrunA17_Chr3g0114511 33.333 150 68 5 9 130 97 242 1.38e-16 74.7
Msa0267520 MtrunA17_Chr4g0063881 33.987 153 62 6 9 129 76 221 2.50e-16 73.9
Msa0267520 MtrunA17_Chr5g0428031 36.620 142 55 5 9 120 74 210 2.96e-16 73.6
Msa0267520 MtrunA17_Chr4g0001331 34.437 151 67 5 9 130 85 232 6.78e-16 72.8
Msa0267520 MtrunA17_Chr3g0107051 34.667 150 66 5 9 129 86 232 8.11e-16 72.4
Msa0267520 MtrunA17_Chr1g0188031 32.624 141 64 3 9 120 87 225 2.85e-15 70.9
Msa0267520 MtrunA17_Chr1g0150321 34.286 140 60 5 9 119 78 214 7.23e-15 69.7
Msa0267520 MtrunA17_Chr2g0284011 32.000 150 70 5 9 129 77 223 4.56e-14 67.4
Msa0267520 MtrunA17_Chr4g0074111 31.507 146 58 5 9 120 83 220 6.43e-14 67.0
Msa0267520 MtrunA17_Chr4g0069301 32.624 141 64 4 9 120 91 229 7.12e-14 67.0
Msa0267520 MtrunA17_Chr8g0379091 32.258 155 69 6 8 131 90 239 1.66e-13 65.9
Msa0267520 MtrunA17_Chr2g0284031 31.126 151 72 5 8 129 95 242 1.81e-13 65.9
Msa0267520 MtrunA17_Chr2g0333821 31.126 151 72 5 9 130 79 226 5.62e-13 64.3
Msa0267520 MtrunA17_Chr4g0059751 34.932 146 60 5 11 129 163 300 1.46e-12 63.2
Msa0267520 MtrunA17_Chr1g0205421 30.464 151 68 5 9 126 77 223 1.88e-12 62.8
Msa0267520 MtrunA17_Chr3g0091281 36.232 138 57 6 9 118 77 211 3.11e-12 61.2
Msa0267520 MtrunA17_Chr8g0385341 34.752 141 61 5 19 131 106 243 4.08e-12 62.0
Msa0267520 MtrunA17_Chr3g0131181 34.507 142 56 5 19 129 105 240 5.54e-12 61.6
Msa0267520 MtrunA17_Chr4g0072831 31.538 130 59 4 9 120 54 171 7.42e-12 60.8
Msa0267520 MtrunA17_Chr7g0267771 32.051 156 75 5 4 131 149 301 1.49e-11 60.5
Msa0267520 MtrunA17_Chr4g0033371 33.071 127 56 4 19 118 148 272 2.78e-11 59.7
Msa0267520 MtrunA17_Chr7g0245031 33.333 135 59 4 11 118 215 345 3.05e-11 59.3
Msa0267520 MtrunA17_Chr8g0351491 29.452 146 70 4 11 129 83 222 3.33e-11 58.9
Msa0267520 MtrunA17_Chr3g0125381 29.730 148 74 4 11 131 19 163 3.87e-11 58.2
Msa0267520 MtrunA17_Chr8g0353621 30.435 138 63 4 19 129 85 216 7.78e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0267520 AT4G33950.2 58.940 151 32 3 9 131 82 230 1.93e-53 171
Msa0267520 AT4G33950.1 58.940 151 32 3 9 131 82 230 3.48e-53 171
Msa0267520 AT1G10940.1 58.940 151 32 3 9 131 65 213 1.22e-52 170
Msa0267520 AT3G50500.1 58.940 151 32 3 9 131 84 232 1.32e-52 169
Msa0267520 AT5G66880.1 58.278 151 33 3 9 131 83 231 1.41e-52 169
Msa0267520 AT5G66880.2 58.278 151 33 3 9 131 83 231 1.41e-52 169
Msa0267520 AT3G50500.2 58.333 156 32 3 9 131 84 239 3.24e-52 169
Msa0267520 AT5G08590.1 59.603 151 31 3 9 131 65 213 4.00e-52 168
Msa0267520 AT1G10940.2 56.604 159 31 3 9 131 65 221 1.10e-51 167
Msa0267520 AT2G23030.1 56.954 151 35 3 9 131 65 213 1.46e-51 166
Msa0267520 AT4G40010.1 59.603 151 31 3 9 131 65 213 1.70e-51 166
Msa0267520 AT1G60940.1 57.616 151 34 3 9 131 65 213 2.65e-51 166
Msa0267520 AT1G60940.2 57.616 151 34 3 9 131 65 213 2.65e-51 166
Msa0267520 AT5G63650.1 58.278 151 33 3 9 131 65 213 7.04e-51 165
Msa0267520 AT1G78290.3 57.237 152 35 3 9 132 65 214 9.13e-51 164
Msa0267520 AT1G78290.2 57.237 152 35 3 9 132 65 214 9.13e-51 164
Msa0267520 AT5G08590.2 58.273 139 28 3 21 131 1 137 1.26e-45 149
Msa0267520 AT5G35410.2 36.913 149 63 4 9 129 76 221 1.39e-18 80.5
Msa0267520 AT5G35410.1 36.913 149 63 4 9 129 76 221 1.51e-18 80.5
Msa0267520 AT1G01140.2 35.664 143 61 4 8 121 83 223 1.67e-18 80.5
Msa0267520 AT1G01140.1 35.664 143 61 4 8 121 83 223 1.71e-18 80.5
Msa0267520 AT1G01140.3 35.664 143 61 4 8 121 83 223 1.74e-18 80.5
Msa0267520 AT3G01090.3 34.194 155 64 4 9 132 84 231 4.99e-18 79.3
Msa0267520 AT3G01090.1 34.194 155 64 4 9 132 84 231 4.99e-18 79.3
Msa0267520 AT3G01090.2 33.553 152 69 3 9 132 107 254 5.57e-18 79.0
Msa0267520 AT4G24400.2 36.429 140 58 4 9 120 74 210 8.65e-18 78.6
Msa0267520 AT1G30270.1 34.507 142 62 4 8 120 95 234 1.04e-17 78.2
Msa0267520 AT4G24400.1 36.429 140 58 4 9 120 74 210 1.06e-17 78.2
Msa0267520 AT1G30270.2 34.507 142 62 4 8 120 95 234 1.18e-17 78.2
Msa0267520 AT5G39440.1 32.432 148 69 3 9 129 84 227 1.60e-17 77.8
Msa0267520 AT3G29160.3 33.548 155 65 4 9 132 85 232 4.34e-17 76.3
Msa0267520 AT5G58380.1 32.667 150 69 5 9 129 77 223 4.59e-17 76.6
Msa0267520 AT2G26980.2 34.211 152 65 5 9 129 79 226 6.67e-17 74.7
Msa0267520 AT3G29160.1 33.548 155 65 4 9 132 85 232 6.80e-17 75.9
Msa0267520 AT3G29160.2 33.548 155 65 4 9 132 85 232 6.80e-17 75.9
Msa0267520 AT1G30270.3 35.036 137 58 4 13 120 3 137 7.21e-17 75.9
Msa0267520 AT2G26980.1 35.664 143 57 5 9 120 79 217 1.72e-16 74.7
Msa0267520 AT2G26980.4 35.664 143 57 5 9 120 89 227 1.83e-16 74.7
Msa0267520 AT2G26980.3 35.664 143 57 5 9 120 79 217 2.01e-16 74.7
Msa0267520 AT2G26980.5 35.664 143 57 5 9 120 79 217 2.07e-16 74.7
Msa0267520 AT5G21222.1 33.333 141 63 4 9 120 78 216 6.20e-16 73.2
Msa0267520 AT5G21222.2 33.333 141 63 4 9 120 78 216 6.20e-16 73.2
Msa0267520 AT5G21222.4 33.333 141 63 4 9 120 78 216 6.20e-16 73.2
Msa0267520 AT5G21222.3 33.333 141 63 4 9 120 78 216 6.20e-16 73.2
Msa0267520 AT3G23000.1 33.113 151 67 4 9 129 91 237 9.22e-16 72.8
Msa0267520 AT5G45820.1 33.775 151 66 6 9 129 77 223 2.43e-15 71.6
Msa0267520 AT5G10930.1 32.667 150 69 5 9 129 78 224 2.57e-14 68.6
Msa0267520 AT5G01810.3 36.111 144 54 7 9 120 77 214 2.90e-14 68.2
Msa0267520 AT5G01810.1 36.111 144 54 7 9 120 77 214 3.36e-14 68.2
Msa0267520 AT5G01810.2 36.111 144 54 7 9 120 77 214 3.36e-14 68.2
Msa0267520 AT3G17510.1 31.757 148 66 5 9 127 85 226 5.93e-14 67.4
Msa0267520 AT5G25110.1 30.667 150 72 5 9 129 108 254 6.26e-14 67.4
Msa0267520 AT5G45810.1 33.099 142 63 5 8 120 92 230 6.56e-14 67.4
Msa0267520 AT4G18700.1 32.857 140 62 5 8 118 90 226 1.95e-13 66.2
Msa0267520 AT1G29230.1 31.788 151 59 6 3 118 133 274 5.65e-13 64.7
Msa0267520 AT4G30960.1 32.237 152 67 6 9 129 89 235 1.02e-12 63.9
Msa0267520 AT5G04510.3 33.750 160 64 7 11 132 111 266 1.51e-12 63.2
Msa0267520 AT5G04510.1 33.540 161 65 7 10 132 110 266 2.28e-12 63.2
Msa0267520 AT1G35670.1 34.286 140 63 4 19 131 102 239 2.88e-12 62.8
Msa0267520 AT5G04510.2 33.540 161 65 7 10 132 110 266 3.05e-12 62.8
Msa0267520 AT3G10540.1 33.533 167 66 8 4 132 108 267 5.81e-12 62.0
Msa0267520 AT5G07070.1 32.000 150 70 5 9 129 77 223 6.51e-12 61.6
Msa0267520 AT4G09570.1 35.461 141 60 5 19 131 101 238 1.14e-11 61.2
Msa0267520 AT1G12580.1 33.824 136 61 4 10 118 111 244 3.20e-11 59.7
Msa0267520 AT3G08730.1 31.852 135 61 3 11 118 201 331 3.49e-11 59.7
Msa0267520 AT3G08730.2 31.852 135 61 3 11 118 201 331 3.49e-11 59.7
Msa0267520 AT3G08720.1 31.852 135 61 3 11 118 207 337 6.55e-11 58.9
Msa0267520 AT3G08720.3 31.852 135 61 3 11 118 207 337 6.55e-11 58.9
Msa0267520 AT3G08720.4 31.852 135 61 3 11 118 207 337 6.55e-11 58.9
Msa0267520 AT3G08720.2 31.852 135 61 3 11 118 207 337 6.55e-11 58.9

Find 37 sgRNAs with CRISPR-Local

Find 117 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGAAATTTCTATGATCTTC+TGG 0.176143 2_2:+69124246 None:intergenic
TCATCATAGAGATTTGAAAT+TGG 0.298226 2_2:-69124781 Msa0267520:CDS
GAAATTTCAGAAAGACTATT+GGG 0.308763 2_2:-69124233 Msa0267520:CDS
AGAAATTTCAGAAAGACTAT+TGG 0.330609 2_2:-69124234 Msa0267520:CDS
GGAGTTCCAACTGTCGCCTT+TGG 0.373657 2_2:+69124583 None:intergenic
TGATCTTCTGGTTCTTCAAA+TGG 0.389191 2_2:+69124258 None:intergenic
GAAATTGGAAAACACTCTCT+TGG 0.399280 2_2:-69124766 Msa0267520:CDS
ATTTCTGTTCTGCACTCTGC+AGG 0.405968 2_2:-69125136 Msa0267520:CDS
TTCTCATCTAGCCATTGTCT+TGG 0.448653 2_2:-69125100 Msa0267520:CDS
TTCATGAGGATAGGCACAAC+CGG 0.498161 2_2:-69125054 Msa0267520:CDS
TTGGAAAACACTCTCTTGGA+TGG 0.513601 2_2:-69124762 Msa0267520:CDS
CAAGATTTAAGCCGAGGGGA+TGG 0.525817 2_2:+69124735 None:intergenic
ATGTGAATTACTATTAACCT+TGG 0.541672 2_2:+69124698 None:intergenic
AAGACAATGGCTAGATGAGA+AGG 0.542672 2_2:+69125102 None:intergenic
TCCTTCATTCAGTGCTCCTG+AGG 0.551255 2_2:-69124563 Msa0267520:intron
GTCATAAAGTGTCACACCAC+AGG 0.555054 2_2:+69124299 None:intergenic
ACCTCAGGAGCACTGAATGA+AGG 0.560856 2_2:+69124562 None:intergenic
CAGTTCTCACTCTGATCCAA+AGG 0.569817 2_2:-69124599 Msa0267520:CDS
ACACTTTATGACATGCTAGT+TGG 0.570446 2_2:-69124288 Msa0267520:CDS
CTCGTCTTCACTAAATCGAC+CGG 0.572882 2_2:+69125035 None:intergenic
TTGCAACTTCGATTACTCCA+AGG 0.574622 2_2:-69124715 Msa0267520:CDS
TCTGATCCAAAGGCGACAGT+TGG 0.577370 2_2:-69124589 Msa0267520:CDS
AGACAATGGCTAGATGAGAA+GGG 0.591203 2_2:+69125103 None:intergenic
ATTGCAGATGTGTGGTCCTG+TGG 0.607065 2_2:-69124315 Msa0267520:CDS
AAGTTGCAAGATTTAAGCCG+AGG 0.609007 2_2:+69124729 None:intergenic
CGGTCGATTTAGTGAAGACG+AGG 0.612886 2_2:-69125034 Msa0267520:intron
GGTGAACTCTTCATGAGGAT+AGG 0.616301 2_2:-69125063 Msa0267520:CDS
AGTTGCAAGATTTAAGCCGA+GGG 0.618700 2_2:+69124730 None:intergenic
TTGTATAGATTGCAGATGTG+TGG 0.620311 2_2:-69124323 Msa0267520:intron
TGGATGGAAATCCATCCCCT+CGG 0.620502 2_2:-69124746 Msa0267520:CDS
ATTGTCTTGGAGTATGCTGA+TGG 0.625672 2_2:-69125087 Msa0267520:CDS
GACAATGGCTAGATGAGAAG+GGG 0.626872 2_2:+69125104 None:intergenic
GTCTTGGAGTATGCTGATGG+TGG 0.656453 2_2:-69125084 Msa0267520:CDS
ATCAGCATACTCCAAGACAA+TGG 0.671309 2_2:+69125089 None:intergenic
AAATTTCAGAAAGACTATTG+GGG 0.682464 2_2:-69124232 None:intergenic
ATGGTGGTGAACTCTTCATG+AGG 0.713505 2_2:-69125068 Msa0267520:CDS
GTTGCAAGATTTAAGCCGAG+GGG 0.717502 2_2:+69124731 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATACGATTTTTTTTTAAA+AGG + chr2_2:69124404-69124423 None:intergenic 10.0%
!!! ATATACGATTTTTTTTTAAA+AGG + chr2_2:69124404-69124423 None:intergenic 10.0%
!! AATATGATATATCTTCAAAA+TGG + chr2_2:69124710-69124729 None:intergenic 15.0%
!! AATATGATATATCTTCAAAA+TGG + chr2_2:69124710-69124729 None:intergenic 15.0%
! TTATTCCAAAAGCTAATATC+TGG - chr2_2:69124478-69124497 Msa0267520:intron 25.0%
!! TCATCATAGAGATTTGAAAT+TGG - chr2_2:69124593-69124612 Msa0267520:CDS 25.0%
! ATGTGAATTACTATTAACCT+TGG + chr2_2:69124679-69124698 None:intergenic 25.0%
! AAAATGATTGATAAAATCCC+AGG + chr2_2:69124869-69124888 None:intergenic 25.0%
!! TTTTATCAATCATTTTCTCG+AGG - chr2_2:69124873-69124892 Msa0267520:intron 25.0%
!! TTTATCAATCATTTTCTCGA+GGG - chr2_2:69124874-69124893 Msa0267520:intron 25.0%
!!! AATTTGAAACGTGGTTTTTA+AGG + chr2_2:69124900-69124919 None:intergenic 25.0%
!!! CAAATTCAAACCACTTTTTA+TGG - chr2_2:69124914-69124933 Msa0267520:intron 25.0%
! AGAAATTTCAGAAAGACTAT+TGG - chr2_2:69125140-69125159 Msa0267520:CDS 25.0%
! GAAATTTCAGAAAGACTATT+GGG - chr2_2:69125141-69125160 Msa0267520:CDS 25.0%
! TTATTCCAAAAGCTAATATC+TGG - chr2_2:69124478-69124497 Msa0267520:intron 25.0%
!! TCATCATAGAGATTTGAAAT+TGG - chr2_2:69124593-69124612 Msa0267520:CDS 25.0%
! ATGTGAATTACTATTAACCT+TGG + chr2_2:69124679-69124698 None:intergenic 25.0%
! AAAATGATTGATAAAATCCC+AGG + chr2_2:69124869-69124888 None:intergenic 25.0%
!! TTTTATCAATCATTTTCTCG+AGG - chr2_2:69124873-69124892 Msa0267520:intron 25.0%
!! TTTATCAATCATTTTCTCGA+GGG - chr2_2:69124874-69124893 Msa0267520:intron 25.0%
!!! AATTTGAAACGTGGTTTTTA+AGG + chr2_2:69124900-69124919 None:intergenic 25.0%
!!! CAAATTCAAACCACTTTTTA+TGG - chr2_2:69124914-69124933 Msa0267520:intron 25.0%
! AGAAATTTCAGAAAGACTAT+TGG - chr2_2:69125140-69125159 Msa0267520:CDS 25.0%
ATCTTGCATGTAGCAAATAT+AGG + chr2_2:69124456-69124475 None:intergenic 30.0%
!!! TAACACCAGATATTAGCTTT+TGG + chr2_2:69124486-69124505 None:intergenic 30.0%
TGTTAGCTACTATCATTCAA+TGG - chr2_2:69124501-69124520 Msa0267520:intron 30.0%
CATGTCTTATATTACGCTTT+AGG - chr2_2:69124566-69124585 Msa0267520:CDS 30.0%
CTGTTAATAGCCATAAAAAG+TGG + chr2_2:69124927-69124946 None:intergenic 30.0%
TTTATGGCTATTAACAGACA+TGG - chr2_2:69124930-69124949 Msa0267520:intron 30.0%
!! GTTGATTCAGTTAGTGTTTT+TGG - chr2_2:69125022-69125041 Msa0267520:intron 30.0%
CTGAAATTTCTATGATCTTC+TGG + chr2_2:69125131-69125150 None:intergenic 30.0%
ATCTTGCATGTAGCAAATAT+AGG + chr2_2:69124456-69124475 None:intergenic 30.0%
!!! TAACACCAGATATTAGCTTT+TGG + chr2_2:69124486-69124505 None:intergenic 30.0%
TGTTAGCTACTATCATTCAA+TGG - chr2_2:69124501-69124520 Msa0267520:intron 30.0%
CATGTCTTATATTACGCTTT+AGG - chr2_2:69124566-69124585 Msa0267520:CDS 30.0%
CTGTTAATAGCCATAAAAAG+TGG + chr2_2:69124927-69124946 None:intergenic 30.0%
TTTATGGCTATTAACAGACA+TGG - chr2_2:69124930-69124949 Msa0267520:intron 30.0%
!! GTTGATTCAGTTAGTGTTTT+TGG - chr2_2:69125022-69125041 Msa0267520:intron 30.0%
CTGAAATTTCTATGATCTTC+TGG + chr2_2:69125131-69125150 None:intergenic 30.0%
CACAAATAATTATGAGCGCA+TGG + chr2_2:69124530-69124549 None:intergenic 35.0%
GAAATTGGAAAACACTCTCT+TGG - chr2_2:69124608-69124627 Msa0267520:intron 35.0%
! CTGTTATGAAAGGAGTACAA+CGG - chr2_2:69124836-69124855 Msa0267520:intron 35.0%
! AGTGGTTTGAATTTGAAACG+TGG + chr2_2:69124909-69124928 None:intergenic 35.0%
TTGTATAGATTGCAGATGTG+TGG - chr2_2:69125051-69125070 Msa0267520:CDS 35.0%
ACACTTTATGACATGCTAGT+TGG - chr2_2:69125086-69125105 Msa0267520:CDS 35.0%
!! TGATCTTCTGGTTCTTCAAA+TGG + chr2_2:69125119-69125138 None:intergenic 35.0%
CACAAATAATTATGAGCGCA+TGG + chr2_2:69124530-69124549 None:intergenic 35.0%
GAAATTGGAAAACACTCTCT+TGG - chr2_2:69124608-69124627 Msa0267520:intron 35.0%
! CTGTTATGAAAGGAGTACAA+CGG - chr2_2:69124836-69124855 Msa0267520:intron 35.0%
! AGTGGTTTGAATTTGAAACG+TGG + chr2_2:69124909-69124928 None:intergenic 35.0%
TTGTATAGATTGCAGATGTG+TGG - chr2_2:69125051-69125070 Msa0267520:CDS 35.0%
ACACTTTATGACATGCTAGT+TGG - chr2_2:69125086-69125105 Msa0267520:CDS 35.0%
!! TGATCTTCTGGTTCTTCAAA+TGG + chr2_2:69125119-69125138 None:intergenic 35.0%
AGACAATGGCTAGATGAGAA+GGG + chr2_2:69124274-69124293 None:intergenic 40.0%
AAGACAATGGCTAGATGAGA+AGG + chr2_2:69124275-69124294 None:intergenic 40.0%
TTCTCATCTAGCCATTGTCT+TGG - chr2_2:69124274-69124293 Msa0267520:CDS 40.0%
ATCAGCATACTCCAAGACAA+TGG + chr2_2:69124288-69124307 None:intergenic 40.0%
ATTGTCTTGGAGTATGCTGA+TGG - chr2_2:69124287-69124306 Msa0267520:CDS 40.0%
TTGGAAAACACTCTCTTGGA+TGG - chr2_2:69124612-69124631 Msa0267520:intron 40.0%
AGTTGCAAGATTTAAGCCGA+GGG + chr2_2:69124647-69124666 None:intergenic 40.0%
AAGTTGCAAGATTTAAGCCG+AGG + chr2_2:69124648-69124667 None:intergenic 40.0%
TTGCAACTTCGATTACTCCA+AGG - chr2_2:69124659-69124678 Msa0267520:intron 40.0%
TCCTGAGGTTCTGTTATGAA+AGG - chr2_2:69124826-69124845 Msa0267520:intron 40.0%
TCCTTTCATAACAGAACCTC+AGG + chr2_2:69124830-69124849 None:intergenic 40.0%
! ATGAAAGGAGTACAACGGAA+AGG - chr2_2:69124841-69124860 Msa0267520:intron 40.0%
AGACAATGGCTAGATGAGAA+GGG + chr2_2:69124274-69124293 None:intergenic 40.0%
AAGACAATGGCTAGATGAGA+AGG + chr2_2:69124275-69124294 None:intergenic 40.0%
TTCTCATCTAGCCATTGTCT+TGG - chr2_2:69124274-69124293 Msa0267520:CDS 40.0%
ATCAGCATACTCCAAGACAA+TGG + chr2_2:69124288-69124307 None:intergenic 40.0%
ATTGTCTTGGAGTATGCTGA+TGG - chr2_2:69124287-69124306 Msa0267520:CDS 40.0%
TTGGAAAACACTCTCTTGGA+TGG - chr2_2:69124612-69124631 Msa0267520:intron 40.0%
AGTTGCAAGATTTAAGCCGA+GGG + chr2_2:69124647-69124666 None:intergenic 40.0%
AAGTTGCAAGATTTAAGCCG+AGG + chr2_2:69124648-69124667 None:intergenic 40.0%
TTGCAACTTCGATTACTCCA+AGG - chr2_2:69124659-69124678 Msa0267520:intron 40.0%
TCCTGAGGTTCTGTTATGAA+AGG - chr2_2:69124826-69124845 Msa0267520:intron 40.0%
TCCTTTCATAACAGAACCTC+AGG + chr2_2:69124830-69124849 None:intergenic 40.0%
! ATGAAAGGAGTACAACGGAA+AGG - chr2_2:69124841-69124860 Msa0267520:intron 40.0%
ATTTCTGTTCTGCACTCTGC+AGG - chr2_2:69124238-69124257 Msa0267520:CDS 45.0%
GACAATGGCTAGATGAGAAG+GGG + chr2_2:69124273-69124292 None:intergenic 45.0%
! ATGGTGGTGAACTCTTCATG+AGG - chr2_2:69124306-69124325 Msa0267520:CDS 45.0%
GGTGAACTCTTCATGAGGAT+AGG - chr2_2:69124311-69124330 Msa0267520:CDS 45.0%
TTCATGAGGATAGGCACAAC+CGG - chr2_2:69124320-69124339 Msa0267520:intron 45.0%
CTCGTCTTCACTAAATCGAC+CGG + chr2_2:69124342-69124361 None:intergenic 45.0%
GTTGCAAGATTTAAGCCGAG+GGG + chr2_2:69124646-69124665 None:intergenic 45.0%
CAGTTCTCACTCTGATCCAA+AGG - chr2_2:69124775-69124794 Msa0267520:CDS 45.0%
!! AGTACAACGGAAAGGTACCT+GGG - chr2_2:69124849-69124868 Msa0267520:intron 45.0%
! GTCATAAAGTGTCACACCAC+AGG + chr2_2:69125078-69125097 None:intergenic 45.0%
ATTTCTGTTCTGCACTCTGC+AGG - chr2_2:69124238-69124257 Msa0267520:CDS 45.0%
GACAATGGCTAGATGAGAAG+GGG + chr2_2:69124273-69124292 None:intergenic 45.0%
! ATGGTGGTGAACTCTTCATG+AGG - chr2_2:69124306-69124325 Msa0267520:CDS 45.0%
GGTGAACTCTTCATGAGGAT+AGG - chr2_2:69124311-69124330 Msa0267520:CDS 45.0%
TTCATGAGGATAGGCACAAC+CGG - chr2_2:69124320-69124339 Msa0267520:intron 45.0%
CTCGTCTTCACTAAATCGAC+CGG + chr2_2:69124342-69124361 None:intergenic 45.0%
GTTGCAAGATTTAAGCCGAG+GGG + chr2_2:69124646-69124665 None:intergenic 45.0%
CAGTTCTCACTCTGATCCAA+AGG - chr2_2:69124775-69124794 Msa0267520:CDS 45.0%
!! AGTACAACGGAAAGGTACCT+GGG - chr2_2:69124849-69124868 Msa0267520:intron 45.0%
! GTCATAAAGTGTCACACCAC+AGG + chr2_2:69125078-69125097 None:intergenic 45.0%
GTCTTGGAGTATGCTGATGG+TGG - chr2_2:69124290-69124309 Msa0267520:CDS 50.0%
! CGGTCGATTTAGTGAAGACG+AGG - chr2_2:69124340-69124359 Msa0267520:intron 50.0%
TGGATGGAAATCCATCCCCT+CGG - chr2_2:69124628-69124647 Msa0267520:intron 50.0%
CAAGATTTAAGCCGAGGGGA+TGG + chr2_2:69124642-69124661 None:intergenic 50.0%
TCTGATCCAAAGGCGACAGT+TGG - chr2_2:69124785-69124804 Msa0267520:CDS 50.0%
TCCTTCATTCAGTGCTCCTG+AGG - chr2_2:69124811-69124830 Msa0267520:intron 50.0%
!! ACCTCAGGAGCACTGAATGA+AGG + chr2_2:69124815-69124834 None:intergenic 50.0%
!! GAGTACAACGGAAAGGTACC+TGG - chr2_2:69124848-69124867 Msa0267520:intron 50.0%
ATTGCAGATGTGTGGTCCTG+TGG - chr2_2:69125059-69125078 Msa0267520:CDS 50.0%
GTCTTGGAGTATGCTGATGG+TGG - chr2_2:69124290-69124309 Msa0267520:CDS 50.0%
! CGGTCGATTTAGTGAAGACG+AGG - chr2_2:69124340-69124359 Msa0267520:intron 50.0%
TGGATGGAAATCCATCCCCT+CGG - chr2_2:69124628-69124647 Msa0267520:intron 50.0%
CAAGATTTAAGCCGAGGGGA+TGG + chr2_2:69124642-69124661 None:intergenic 50.0%
TCTGATCCAAAGGCGACAGT+TGG - chr2_2:69124785-69124804 Msa0267520:CDS 50.0%
TCCTTCATTCAGTGCTCCTG+AGG - chr2_2:69124811-69124830 Msa0267520:intron 50.0%
!! ACCTCAGGAGCACTGAATGA+AGG + chr2_2:69124815-69124834 None:intergenic 50.0%
!! GAGTACAACGGAAAGGTACC+TGG - chr2_2:69124848-69124867 Msa0267520:intron 50.0%
ATTGCAGATGTGTGGTCCTG+TGG - chr2_2:69125059-69125078 Msa0267520:CDS 50.0%
GGAGTTCCAACTGTCGCCTT+TGG + chr2_2:69124794-69124813 None:intergenic 55.0%
GGAGTTCCAACTGTCGCCTT+TGG + chr2_2:69124794-69124813 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 69124233 69125163 69124233 ID=Msa0267520;Name=Msa0267520
chr2_2 mRNA 69124233 69125163 69124233 ID=Msa0267520-mRNA-1;Parent=Msa0267520;Name=Msa0267520-mRNA-1;_AED=0.17;_eAED=0.17;_QI=0|0|0|1|1|0.75|4|0|132
chr2_2 exon 69125035 69125163 69125035 ID=Msa0267520-mRNA-1:exon:22044;Parent=Msa0267520-mRNA-1
chr2_2 exon 69124709 69124808 69124709 ID=Msa0267520-mRNA-1:exon:22043;Parent=Msa0267520-mRNA-1
chr2_2 exon 69124564 69124625 69124564 ID=Msa0267520-mRNA-1:exon:22042;Parent=Msa0267520-mRNA-1
chr2_2 exon 69124233 69124337 69124233 ID=Msa0267520-mRNA-1:exon:22041;Parent=Msa0267520-mRNA-1
chr2_2 CDS 69125035 69125163 69125035 ID=Msa0267520-mRNA-1:cds;Parent=Msa0267520-mRNA-1
chr2_2 CDS 69124709 69124808 69124709 ID=Msa0267520-mRNA-1:cds;Parent=Msa0267520-mRNA-1
chr2_2 CDS 69124564 69124625 69124564 ID=Msa0267520-mRNA-1:cds;Parent=Msa0267520-mRNA-1
chr2_2 CDS 69124233 69124337 69124233 ID=Msa0267520-mRNA-1:cds;Parent=Msa0267520-mRNA-1
Gene Sequence

>Msa0267520

ATGAGATTTCTGTTCTGCACTCTGCAGGTCTTTCTTACCCCTTCTCATCTAGCCATTGTCTTGGAGTATGCTGATGGTGGTGAACTCTTCATGAGGATAGGCACAACCGGTCGATTTAGTGAAGACGAGGAAATTCATCATAGAGATTTGAAATTGGAAAACACTCTCTTGGATGGAAATCCATCCCCTCGGCTTAAATCTTGCAACTTCGATTACTCCAAGGTTAATATCTGCAGTTCTCACTCTGATCCAAAGGCGACAGTTGGAACTCCTTCATTCAGTGCTCCTGAGATTGCAGATGTGTGGTCCTGTGGTGTGACACTTTATGACATGCTAGTTGGTGCATATCCATTTGAAGAACCAGAAGATCATAGAAATTTCAGAAAGACTATTGGG
Protein sequence

>Msa0267520

MRFLFCTLQVFLTPSHLAIVLEYADGGELFMRIGTTGRFSEDEEIHHRDLKLENTLLDGNPSPRLKSCNFDYSKVNICSSHSDPKATVGTPSFSAPEIADVWSCGVTLYDMLVGAYPFEEPEDHRNFRKTIG