AlfalfaGEDB | Msa0267570

M. sativa cultivar zhongmu-4 / Msa0267570

KEGG: K13415
GO: GO:0003002 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009733 GO:0009734 GO:0009741 GO:0009742 GO:0009755 GO:0009987 GO:0010033 GO:0010051 GO:0010232 GO:0010233 GO:0010305 GO:0014070 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0032501 GO:0032502 GO:0032870 GO:0033993 GO:0036211 GO:0038023 GO:0042221 GO:0043170 GO:0043401 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0048545 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0060089 GO:0065007 GO:0070887 GO:0071310 GO:0071365 GO:0071367 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0140096 GO:1901564 GO:1901700 GO:1901701
PFAM: PF07714.20

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0267570	RVW34595.1	64.078	103	14	2	1	80	1	103	5.97e-32	120

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0267570	sp\|Q9ZPS9\|BRL2_ARATH	44.776	134	20	2	1	80	880	1013	1.57e-24	98.6

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0267570	A0A438DGK0	64.078	103	14	2	1	80	1	103	2.85e-32	120

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0267570	MtrunA17_Chr2g0285131	52.985	134	9	2	1	80	1	134	3.92e-34	116
Msa0267570	MtrunA17_Chr8g0343941	52.239	134	10	2	1	80	1	134	4.92e-34	115
Msa0267570	MtrunA17_Chr8g0375511	51.493	134	11	2	1	80	882	1015	2.10e-32	117
Msa0267570	MtrunA17_Chr3g0145631	51.493	134	11	2	1	80	1	134	2.10e-32	111
Msa0267570	MtrunA17_Chr4g0028031	69.565	69	21	0	1	69	1	69	1.17e-26	93.2
Msa0267570	MtrunA17_Chr3g0079661	41.791	134	21	3	1	80	958	1088	1.55e-23	92.4
Msa0267570	MtrunA17_Chr3g0129391	42.748	131	23	3	2	80	918	1048	1.01e-20	84.3
Msa0267570	MtrunA17_Chr1g0203631	60.000	60	22	1	1	60	1035	1092	1.73e-14	66.6
Msa0267570	MtrunA17_Chr7g0239361	43.333	60	31	1	1	60	400	456	1.35e-11	58.5
Msa0267570	MtrunA17_Chr3g0112111	43.333	60	30	1	1	60	392	447	3.10e-11	57.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0267570	AT2G01950.1	44.776	134	20	2	1	80	880	1013	1.60e-25	98.6
Msa0267570	AT4G39400.1	41.667	132	25	3	1	80	925	1056	3.11e-21	86.3
Msa0267570	AT1G55610.1	40.299	134	26	2	1	80	901	1034	5.80e-21	85.5
Msa0267570	AT1G55610.2	40.299	134	26	2	1	80	901	1034	5.80e-21	85.5
Msa0267570	AT3G13380.1	73.469	49	13	0	1	49	900	948	9.39e-20	82.0
Msa0267570	AT5G07280.1	55.000	60	25	1	1	60	959	1016	6.08e-12	59.7
Msa0267570	AT3G59730.1	45.000	60	33	0	1	60	378	437	1.41e-11	58.5
Msa0267570	AT3G59740.1	43.333	60	34	0	1	60	376	435	8.05e-11	56.6

Find 16 sgRNAs with CRISPR-Local

Find 23 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTTTATGAATGCATGGAATA+TGG	0.304945	2_2:+69199014	Msa0267570:CDS
ACTTTGCAGTATCCCAATAA+AGG	0.324031	2_2:-69198972	None:intergenic
CCTCTTCCCATGTTAAAATT+CGG	0.341990	2_2:-69199082	None:intergenic
CTTTGCAGTATCCCAATAAA+GGG	0.404765	2_2:-69198971	None:intergenic
TTATTGGGATACTGCAAAGT+AGG	0.482244	2_2:+69198975	Msa0267570:CDS
AACTCTGGTGGAACATATCC+AGG	0.488834	2_2:-69199232	None:intergenic
CACATGTCTTTATTCTTCTG+TGG	0.497855	2_2:-69199055	None:intergenic
TCTACTTGTTTATGAATGCA+TGG	0.509838	2_2:+69199007	Msa0267570:CDS
GCCTCTAATTAAATCAACTC+TGG	0.518536	2_2:-69199247	None:intergenic
AGCACACTAGCAGGAACACC+TGG	0.526989	2_2:+69199214	Msa0267570:CDS
CTCAGTGTAAGCACACTAGC+AGG	0.536536	2_2:+69199205	Msa0267570:intron
TCTAATTAAATCAACTCTGG+TGG	0.544384	2_2:-69199244	None:intergenic
TAGATAATTCATGGCTGAGA+TGG	0.571816	2_2:+69198914	None:intergenic
AGTATCCCAATAAAGGGGCA+AGG	0.622299	2_2:-69198965	None:intergenic
AAGAGGAAGAAGATTGCAAG+AGG	0.622806	2_2:+69199099	Msa0267570:CDS
TTTGCAGTATCCCAATAAAG+GGG	0.688128	2_2:-69198970	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTATGCTTGATTTTTTCTA+AGG	-	chr2_2:69198943-69198962	None:intergenic	20.0%
	TCTACTTGTTTATGAATGCA+TGG	+	chr2_2:69199007-69199026	Msa0267570:CDS	30.0%
	GTTTATGAATGCATGGAATA+TGG	+	chr2_2:69199014-69199033	Msa0267570:CDS	30.0%
	TAAAAACCTTGCCCCTTTAT+TGG	+	chr2_2:69198959-69198978	Msa0267570:CDS	35.0%
	AAAAACCTTGCCCCTTTATT+GGG	+	chr2_2:69198960-69198979	Msa0267570:CDS	35.0%
	TTTGCAGTATCCCAATAAAG+GGG	-	chr2_2:69198973-69198992	None:intergenic	35.0%
	CTTTGCAGTATCCCAATAAA+GGG	-	chr2_2:69198974-69198993	None:intergenic	35.0%
	ACTTTGCAGTATCCCAATAA+AGG	-	chr2_2:69198975-69198994	None:intergenic	35.0%
	TTATTGGGATACTGCAAAGT+AGG	+	chr2_2:69198975-69198994	Msa0267570:CDS	35.0%
	CACATGTCTTTATTCTTCTG+TGG	-	chr2_2:69199058-69199077	None:intergenic	35.0%
!!	TGATCGCCGAATTTTAACAT+GGG	+	chr2_2:69199076-69199095	Msa0267570:CDS	35.0%
	CCTCTTCCCATGTTAAAATT+CGG	-	chr2_2:69199085-69199104	None:intergenic	35.0%
	TGTCTTTGTGTATGATGTGA+GGG	-	chr2_2:69199164-69199183	None:intergenic	35.0%
	ATGTCTTTGTGTATGATGTG+AGG	-	chr2_2:69199165-69199184	None:intergenic	35.0%
!!	TTCTGTGGAGCATCTTTTCT+AGG	-	chr2_2:69199043-69199062	None:intergenic	40.0%
!!	GTGATCGCCGAATTTTAACA+TGG	+	chr2_2:69199075-69199094	Msa0267570:CDS	40.0%
!!	CCGAATTTTAACATGGGAAG+AGG	+	chr2_2:69199082-69199101	Msa0267570:CDS	40.0%
	AAGAGGAAGAAGATTGCAAG+AGG	+	chr2_2:69199099-69199118	Msa0267570:CDS	40.0%
	AGTATCCCAATAAAGGGGCA+AGG	-	chr2_2:69198968-69198987	None:intergenic	45.0%
!!	ATTGCAAGAGGTGCTGCTAA+AGG	+	chr2_2:69199111-69199130	Msa0267570:intron	45.0%
	AACTCTGGTGGAACATATCC+AGG	-	chr2_2:69199235-69199254	None:intergenic	45.0%
	CTCAGTGTAAGCACACTAGC+AGG	+	chr2_2:69199205-69199224	Msa0267570:intron	50.0%
	AGCACACTAGCAGGAACACC+TGG	+	chr2_2:69199214-69199233	Msa0267570:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr2_2	gene	69198924	69199261	69198924	ID=Msa0267570;Name=Msa0267570
chr2_2	mRNA	69198924	69199261	69198924	ID=Msa0267570-mRNA-1;Parent=Msa0267570;Name=Msa0267570-mRNA-1;_AED=0.32;_eAED=0.32;_QI=0\|0\|0\|1\|1\|1\|2\|0\|82
chr2_2	exon	69198924	69199120	69198924	ID=Msa0267570-mRNA-1:exon:22057;Parent=Msa0267570-mRNA-1
chr2_2	exon	69199210	69199261	69199210	ID=Msa0267570-mRNA-1:exon:22058;Parent=Msa0267570-mRNA-1
chr2_2	CDS	69198924	69199120	69198924	ID=Msa0267570-mRNA-1:cds;Parent=Msa0267570-mRNA-1
chr2_2	CDS	69199210	69199261	69199210	ID=Msa0267570-mRNA-1:cds;Parent=Msa0267570-mRNA-1

Gene Sequence	>Msa0267570 ATGGCTGAGATGGAAACCTTAGAAAAAATCAAGCATAAAAACCTTGCCCCTTTATTGGGATACTGCAAAGTAGGAGAAGAGATTCTACTTGTTTATGAATGCATGGAATATGGAAGCCTAGAAAAGATGCTCCACAGAAGAATAAAGACATGTGATCGCCGAATTTTAACATGGGAAGAGGAAGAAGATTGCAAGAGTGTAAGCACACTAGCAGGAACACCTGGATATGTTCCACCAGAGTTGATTTAA
Protein sequence	>Msa0267570 MAEMETLEKIKHKNLAPLLGYCKVGEEILLVYECMEYGSLEKMLHRRIKTCDRRILTWEEEEDCKSVSTLAGTPGYVPPELI*