AlfalfaGEDB | Msa0275990

M. sativa cultivar zhongmu-4 / Msa0275990

KEGG: -
GO: -
PFAM: PF03101.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0275990	XP_024636348.1	91.775	693	29	1	1	665	1	693	0.0	1321

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0275990	sp\|Q9SZL8\|FRS5_ARATH	34.783	529	310	9	62	557	117	643	9.12e-101	328

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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TFs/TRs:

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Gene ID	Type	Classification
Msa0275990	TF	FAR1

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0275990	MtrunA17_Chr8g0363841	68.899	672	179	2	1	644	1	670	0.0	960
Msa0275990	MtrunA17_Chr1g0202341	32.612	647	381	14	20	632	54	679	7.55e-107	338
Msa0275990	MtrunA17_Chr2g0324011	32.302	582	347	10	19	563	12	583	8.66e-101	320
Msa0275990	MtrunA17_Chr5g0448761	31.544	596	363	12	62	621	104	690	1.45e-98	320
Msa0275990	MtrunA17_Chr3g0082121	34.426	549	319	11	29	549	55	590	2.25e-97	314
Msa0275990	MtrunA17_Chr6g0458391	30.940	585	337	12	1	549	212	765	1.87e-90	298
Msa0275990	MtrunA17_Chr2g0287651	31.418	557	339	8	27	549	85	632	2.69e-89	297
Msa0275990	MtrunA17_Chr2g0296781	32.998	497	296	6	87	549	63	556	9.89e-84	279
Msa0275990	MtrunA17_Chr5g0440451	28.899	654	384	16	18	624	44	663	1.00e-83	283
Msa0275990	MtrunA17_Chr1g0207551	30.935	556	341	8	27	549	44	589	4.50e-82	276

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0275990	AT4G38180.1	34.783	529	310	9	62	557	117	643	9.27e-102	328
Msa0275990	AT4G15090.1	31.984	494	304	7	88	552	122	612	3.41e-81	275
Msa0275990	AT4G15090.5	31.984	494	304	7	88	552	122	612	3.41e-81	275
Msa0275990	AT4G15090.3	31.984	494	304	7	88	552	122	612	3.41e-81	275
Msa0275990	AT4G15090.2	31.984	494	304	7	88	552	122	612	3.41e-81	275
Msa0275990	AT4G15090.4	31.984	494	304	7	88	552	122	612	3.41e-81	275
Msa0275990	AT2G27110.2	31.151	504	304	7	90	564	107	596	7.38e-81	274
Msa0275990	AT2G27110.1	31.151	504	304	7	90	564	107	596	7.38e-81	274
Msa0275990	AT1G76320.3	30.545	550	343	9	27	549	7	544	6.63e-75	254
Msa0275990	AT1G76320.1	30.545	550	343	9	27	549	7	544	1.88e-74	254

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Find 103 sgRNAs with CRISPR-Local

Find 268 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGTTGATTATGATTGCTT+TGG	0.341170	2_3:+8150681	Msa0275990:CDS
AAATATGTCAAATGTCAAAC+GGG	0.464386	2_3:+8150380	Msa0275990:CDS
AAATATTTGGAATTATTTAG+GGG	0.632966	2_3:+8150443	Msa0275990:CDS
AACAAATGTCTTTGCTCAGG+AGG	0.641459	2_3:-8150345	None:intergenic
AACAGTAAAACTCTTTAAAA+CGG	0.389800	2_3:-8150164	None:intergenic
AACATCTTGTTAAGATGTAT+TGG	0.361271	2_3:-8151683	None:intergenic
AAGAAGAAATTCAAACCACT+TGG	0.543532	2_3:+8151075	Msa0275990:CDS
AAGACTTCAGAATAGCGTTG+CGG	0.703840	2_3:-8151809	None:intergenic
AAGGACAAATTGCAAAGCTA+TGG	0.542290	2_3:+8150248	Msa0275990:CDS
AAGTTGAAATTGATTGAAGC+TGG	0.434164	2_3:+8150534	Msa0275990:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGTCAATCAGTTGAAATTGA+TGG	+	chr2_3:8150003-8150022	Msa0275990:CDS	30.0%
ATTTAGTGTAAGAAAAGGGA+GGG	+	chr2_3:8150110-8150129	Msa0275990:CDS	30.0%
GACATTTGACATATTTCTCA+TGG	-	chr2_3:8150376-8150395	None:intergenic	30.0%
AATTCACCATAGACTTTATG+AGG	-	chr2_3:8150407-8150426	None:intergenic	30.0%
ATAAAGTCTATGGTGAATTG+TGG	+	chr2_3:8150408-8150427	Msa0275990:CDS	30.0%
AAGTTGAAATTGATTGAAGC+TGG	+	chr2_3:8150534-8150553	Msa0275990:CDS	30.0%
AGTTGAAATTGATTGAAGCT+GGG	+	chr2_3:8150535-8150554	Msa0275990:CDS	30.0%
AGTTGATTATGATTGCTTTG+GGG	+	chr2_3:8150683-8150702	Msa0275990:CDS	30.0%
ATGATTTATCCCAACAAATG+GGG	-	chr2_3:8150763-8150782	None:intergenic	30.0%
GATGATTTATCCCAACAAAT+GGG	-	chr2_3:8150764-8150783	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_3	gene	8149988	8152085	8149988	ID=Msa0275990;Name=Msa0275990
chr2_3	mRNA	8149988	8152085	8149988	ID=Msa0275990-mRNA-1;Parent=Msa0275990;Name=Msa0275990-mRNA-1;_AED=0.29;_eAED=0.29;_QI=0\|0\|0\|0.5\|1\|1\|2\|0\|670
chr2_3	exon	8149988	8151386	8149988	ID=Msa0275990-mRNA-1:exon:3711;Parent=Msa0275990-mRNA-1
chr2_3	exon	8151472	8152085	8151472	ID=Msa0275990-mRNA-1:exon:3712;Parent=Msa0275990-mRNA-1
chr2_3	CDS	8149988	8151386	8149988	ID=Msa0275990-mRNA-1:cds;Parent=Msa0275990-mRNA-1
chr2_3	CDS	8151472	8152085	8151472	ID=Msa0275990-mRNA-1:cds;Parent=Msa0275990-mRNA-1

Gene Sequence

>Msa0275990

ATGGAGATAAATGAATGTCAATCAGTTGAAATTGATGGATGCAAAGATATTTTTCTTGGTCAAGTTGTGAATAACAAAGATGAAGCTTATAACTTATATCAAGCACATGCCTTCAAAACAAGATTTAGTGTAAGAAAAGGGAGGGAGTTATATTATGATAATGAGAGAAAAATCACCCGTTTTAAAGAGTTTTACTGTTTCAAAGAAGGGTTTAAGAATAATGAATATGGAGGTGAAGTGGCTTATGAAAGAGCTGATTCAAGGACAAATTGCAAAGCTATGGCGAGGATTAATGTTAGCAAGGAAGGAGTTTGGAAAGTTACCAAGTTTGTATTAGATCATAATCATCAACTTGTACCTCCTGAGCAAAGACATTTGTTGCGGTCCATGAGAAATATGTCAAATGTCAAACGGGACCTCATAAAGTCTATGGTGAATTGTGGTATACGAGTTACAAATATTTGGAATTATTTAGGGGAAGAGGTCGGTGGATATAACAATCTTGGTATAACATTGAAAGATATGCATAATTATGTCTACACTGAAAAGTTGAAATTGATTGAAGCTGGGGATGCTCAATCTTTAGTAAATAACTTACAAAATAGACAAGCAAAAGATCCTATGTTCTATTACAGTGTGCAGCTAGATCAGGAATCTCGTTTGACTAATGTCTTTTGGAGAGATGGTAAATCTAAAGTTGATTATGATTGCTTTGGGGATGTTGTGATATTTGACACTATTTATCGTACCAATAAGTATGACATGATTTGTGCCCCATTTGTTGGGATAAATCATCATTGGCAAAATGTCATGTTTGGTTGTGCTTTGTTGTTGGATGAAACTTCAATTTCTTTTAATTGGTTGTTTAAAGCATTTTTAGAGAGTATGGGAAACAAACAACCAAAAACAATATTTACAGATCAAGATCCAGCTATGGGCAAAGCAATTGGAGAAGTAATGCCAAATACACGTCATCGACTGTGTTTGTGGCATATATCAAAAAATGCTCCTTCACATTTGGGATCTCTCAATTCTAATAACAAGTTTCAAAGCTTATTTAATAAATGCATGACGGGTTGTGACTCAGAAGAAGAAATTCAAACCACTTGGGATGAGATGATGAATACATATCAACTTCATGGTCACAAATGGTTAAGAAGCATGTATGCAATTCGCATCAAATGGAGCACGACATATAGTAATCATGTATTTTCTGCTGGTATCAAATCTTCTCAGAGGAGTGAGAGTACAAATAGTGCTTTAGGAGATATTGCAGGAAAAACAACATGTCTCTCACAATTTTTAGTGGCATTTGAGAAGATGGTGAAAAGGTGGCGACAATTAGAGGTTGAGAACGAATTCAAAAATAGTCAGAGTGCGCCTCCTCGTGTTATAAACAGTGGAAGCACTTCCGTGGAGATTGGTCGATGTGATAATGTGTCATATCATGAAGTTGTGTTGAACATCATCCCCAATAAAAAGTATATCGTGACTTTGGACTCATCCACTTCTATGGTTAGTTGCAGTTGTCACAAGTTCGAATCTATGGGCATTCTTTGCAGTCATGCTTTGCGGATTTACAATATTAAAGGTATTTTAAAAATACCTGACCAATACATCTTAACAAGATGTTCAAAAAATGCAAGGTCTGTCATTTATGAGCATGTTAATAAGAGTTCAGAAGAAGATTCTACCTCCAATTCAATGTCTGATGGTGATGATTCCGGTCTTCTTTACCGCAACGCTATTCTGAAGTCTTTATACAATTTAGTTTTGGAATCCCAGGAGGACAAAAAAGCACAAAAAATCATGTGGGAATTGCTTGATATTGGAGTTAAGAGGGTTCGAGAATGTGTCAGCAAACCTGACTTAAATTCAACTGAAGAAAACATTGAGAACAATTTCTCACAAAATGAGGATAATGAAAGCTGTACAGTTTTGAATCCTCGTTTCGCAAAGACGAAAGGAAGGTCTAGTGTTCGCCAGAAAGGACATTTTGAGAAAAGAAAATGA

Protein sequence

>Msa0275990

MEINECQSVEIDGCKDIFLGQVVNNKDEAYNLYQAHAFKTRFSVRKGRELYYDNERKITRFKEFYCFKEGFKNNEYGGEVAYERADSRTNCKAMARINVSKEGVWKVTKFVLDHNHQLVPPEQRHLLRSMRNMSNVKRDLIKSMVNCGIRVTNIWNYLGEEVGGYNNLGITLKDMHNYVYTEKLKLIEAGDAQSLVNNLQNRQAKDPMFYYSVQLDQESRLTNVFWRDGKSKVDYDCFGDVVIFDTIYRTNKYDMICAPFVGINHHWQNVMFGCALLLDETSISFNWLFKAFLESMGNKQPKTIFTDQDPAMGKAIGEVMPNTRHRLCLWHISKNAPSHLGSLNSNNKFQSLFNKCMTGCDSEEEIQTTWDEMMNTYQLHGHKWLRSMYAIRIKWSTTYSNHVFSAGIKSSQRSESTNSALGDIAGKTTCLSQFLVAFEKMVKRWRQLEVENEFKNSQSAPPRVINSGSTSVEIGRCDNVSYHEVVLNIIPNKKYIVTLDSSTSMVSCSCHKFESMGILCSHALRIYNIKGILKIPDQYILTRCSKNARSVIYEHVNKSSEEDSTSNSMSDGDDSGLLYRNAILKSLYNLVLESQEDKKAQKIMWELLDIGVKRVRECVSKPDLNSTEENIENNFSQNEDNESCTVLNPRFAKTKGRSSVRQKGHFEKRK*