AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0276160

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0276160 MtrunA17_Chr2g0288601 88.298 94 11 0 12 105 29 122 2.61e-58 178
Msa0276160 MtrunA17_Chr7g0243081 82.609 69 12 0 42 110 59 127 9.21e-36 127
Msa0276160 MtrunA17_Chr1g0210911 77.143 70 16 0 43 112 11 80 8.23e-35 124
Msa0276160 MtrunA17_Chr2g0320231 66.265 83 28 0 42 124 85 167 4.59e-34 123
Msa0276160 MtrunA17_Chr1g0162931 78.788 66 14 0 45 110 9 74 6.90e-34 120
Msa0276160 MtrunA17_Chr8g0379121 76.471 68 16 0 46 113 103 170 8.09e-33 120
Msa0276160 MtrunA17_Chr2g0283951 68.493 73 19 1 46 114 81 153 6.55e-28 106
Msa0276160 MtrunA17_Chr3g0095611 55.714 70 31 0 46 115 11 80 5.58e-24 93.2
Msa0276160 MtrunA17_Chr1g0154261 65.672 67 22 1 45 110 116 182 2.17e-22 90.5
Msa0276160 MtrunA17_Chr7g0261911 43.243 111 57 2 28 132 231 341 7.86e-22 90.9
Msa0276160 MtrunA17_Chr8g0366281 36.585 164 87 3 18 164 136 299 1.17e-21 89.4
Msa0276160 MtrunA17_Chr6g0479431 57.143 70 30 0 53 122 201 270 1.34e-21 89.4
Msa0276160 MtrunA17_Chr5g0410741 48.913 92 46 1 48 138 212 303 2.33e-21 89.4
Msa0276160 MtrunA17_Chr3g0109981 48.387 93 46 2 48 138 267 359 9.23e-21 87.8
Msa0276160 MtrunA17_Chr1g0190301 64.286 56 20 0 55 110 176 231 9.52e-21 87.4
Msa0276160 MtrunA17_Chr7g0274471 57.971 69 27 1 55 121 219 287 1.04e-20 87.4
Msa0276160 MtrunA17_Chr6g0461641 64.151 53 19 0 55 107 43 95 8.22e-19 82.0
Msa0276160 MtrunA17_Chr7g0254981 45.283 106 55 2 46 149 42 146 9.32e-19 80.5
Msa0276160 MtrunA17_Chr7g0258551 60.656 61 24 0 50 110 208 268 1.50e-18 81.3
Msa0276160 MtrunA17_Chr1g0197171 63.158 57 21 0 55 111 45 101 1.89e-18 80.1
Msa0276160 MtrunA17_Chr5g0409731 62.069 58 22 0 50 107 21 78 2.21e-18 79.7
Msa0276160 MtrunA17_Chr2g0290991 63.158 57 21 0 55 111 40 96 2.54e-18 80.1
Msa0276160 MtrunA17_Chr2g0321461 62.264 53 20 0 55 107 46 98 6.91e-18 79.3
Msa0276160 MtrunA17_Chr5g0417521 49.254 67 34 0 55 121 16 82 9.67e-18 78.6
Msa0276160 MtrunA17_Chr1g0175091 63.636 55 20 0 56 110 260 314 1.47e-17 78.6
Msa0276160 MtrunA17_Chr1g0175081 50.746 67 33 0 44 110 12 78 2.21e-17 73.2
Msa0276160 MtrunA17_Chr2g0291021 65.455 55 19 0 56 110 51 105 4.51e-17 76.6
Msa0276160 MtrunA17_Chr2g0310011 41.121 107 49 3 55 155 36 134 4.92e-17 76.6
Msa0276160 MtrunA17_Chr8g0356501 39.450 109 54 2 18 120 7 109 6.57e-17 76.3
Msa0276160 MtrunA17_Chr4g0042451 50.000 64 31 1 55 118 18 80 6.08e-16 73.6
Msa0276160 MtrunA17_Chr4g0045361 50.000 68 34 0 40 107 260 327 1.77e-15 72.8
Msa0276160 MtrunA17_Chr1g0175071 58.491 53 22 0 56 108 179 231 1.23e-14 69.7
Msa0276160 MtrunA17_Chr7g0242521 61.818 55 18 1 56 107 15 69 2.82e-14 67.8
Msa0276160 MtrunA17_Chr7g0258531 36.296 135 63 4 14 132 171 298 9.87e-14 67.4
Msa0276160 MtrunA17_Chr4g0062921 57.692 52 22 0 56 107 237 288 1.49e-13 67.4
Msa0276160 MtrunA17_Chr2g0304841 54.545 55 24 1 53 107 212 265 3.27e-13 66.2
Msa0276160 MtrunA17_Chr4g0031961 50.794 63 30 1 45 107 122 183 6.43e-12 62.0
Msa0276160 MtrunA17_Chr7g0275401 54.545 55 24 1 53 107 191 244 7.63e-12 62.0
Msa0276160 MtrunA17_Chr1g0198601 53.061 49 23 0 59 107 216 264 8.88e-12 62.0
Msa0276160 MtrunA17_Chr5g0422621 50.000 52 26 0 56 107 200 251 1.51e-11 61.2
Msa0276160 MtrunA17_Chr7g0255711 38.000 100 53 3 53 144 90 188 2.44e-11 60.5
Msa0276160 MtrunA17_Chr5g0402631 51.786 56 27 0 52 107 212 267 3.63e-11 60.1
Msa0276160 MtrunA17_Chr4g0009901 46.429 56 29 1 52 107 166 220 4.25e-11 59.7
Msa0276160 MtrunA17_Chr1g0149841 52.727 55 25 1 53 107 190 243 9.11e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0276160 AT2G38300.1 57.025 121 48 2 29 145 29 149 3.74e-36 128
Msa0276160 AT4G04605.1 66.265 83 25 1 41 120 1 83 1.02e-34 121
Msa0276160 AT2G02060.2 82.540 63 11 0 46 108 21 83 2.40e-33 119
Msa0276160 AT2G02060.1 82.540 63 11 0 46 108 21 83 2.40e-33 119
Msa0276160 AT2G40260.1 63.529 85 31 0 33 117 61 145 3.65e-32 119
Msa0276160 AT2G42660.1 76.119 67 16 0 46 112 42 108 7.18e-31 112
Msa0276160 AT1G14600.1 75.385 65 16 0 46 110 15 79 4.91e-30 110
Msa0276160 AT4G04555.1 57.534 73 31 0 41 113 1 73 4.02e-26 95.9
Msa0276160 AT4G04580.1 54.795 73 33 0 41 113 1 73 4.70e-25 95.1
Msa0276160 AT5G42630.2 68.966 58 18 0 53 110 104 161 1.80e-22 89.7
Msa0276160 AT4G17695.1 49.474 95 41 1 16 110 133 220 2.04e-22 91.7
Msa0276160 AT5G42630.1 68.966 58 18 0 53 110 104 161 2.28e-22 90.5
Msa0276160 AT1G32240.2 64.062 64 22 1 48 110 206 269 1.31e-20 87.0
Msa0276160 AT1G32240.1 64.062 64 22 1 48 110 206 269 1.44e-20 87.4
Msa0276160 AT5G16560.1 64.062 64 22 1 48 110 212 275 1.71e-20 87.0
Msa0276160 AT4G28610.1 65.455 55 19 0 56 110 227 281 1.86e-19 84.3
Msa0276160 AT5G29000.1 65.455 55 19 0 56 110 190 244 2.30e-19 84.0
Msa0276160 AT5G29000.3 65.455 55 19 0 56 110 190 244 2.30e-19 84.0
Msa0276160 AT5G29000.4 54.412 68 31 0 56 123 233 300 2.58e-19 83.2
Msa0276160 AT5G29000.2 65.455 55 19 0 56 110 233 287 2.94e-19 84.0
Msa0276160 AT3G04450.4 42.105 95 54 1 56 149 200 294 4.40e-19 83.2
Msa0276160 AT3G04450.2 42.105 95 54 1 56 149 200 294 4.40e-19 83.2
Msa0276160 AT3G04450.1 42.105 95 54 1 56 149 240 334 4.75e-19 83.2
Msa0276160 AT3G04450.3 61.818 55 21 0 56 110 240 294 5.06e-19 82.4
Msa0276160 AT5G45580.1 58.730 63 26 0 50 112 3 65 6.51e-19 81.3
Msa0276160 AT5G45580.2 58.730 63 26 0 50 112 19 81 7.54e-19 81.3
Msa0276160 AT3G13040.3 49.383 81 41 0 56 136 243 323 3.89e-18 80.9
Msa0276160 AT3G13040.1 49.383 81 41 0 56 136 243 323 3.89e-18 80.9
Msa0276160 AT3G13040.2 49.383 81 41 0 56 136 243 323 3.89e-18 80.9
Msa0276160 AT2G20400.2 63.636 55 20 0 56 110 233 287 4.93e-18 80.1
Msa0276160 AT2G20400.1 63.636 55 20 0 56 110 233 287 5.05e-18 80.1
Msa0276160 AT5G18240.2 62.264 53 20 0 55 107 46 98 6.19e-18 80.1
Msa0276160 AT5G18240.3 62.264 53 20 0 55 107 46 98 6.19e-18 80.1
Msa0276160 AT5G18240.5 62.264 53 20 0 55 107 46 98 7.23e-18 79.7
Msa0276160 AT3G04030.1 60.377 53 21 0 55 107 46 98 7.42e-18 79.7
Msa0276160 AT5G18240.1 62.264 53 20 0 55 107 46 98 7.43e-18 79.7
Msa0276160 AT5G18240.4 62.264 53 20 0 55 107 46 98 7.43e-18 79.7
Msa0276160 AT3G04030.3 60.377 53 21 0 55 107 46 98 8.76e-18 79.7
Msa0276160 AT3G12730.1 59.649 57 23 0 55 111 24 80 1.36e-17 77.0
Msa0276160 AT2G01060.1 65.455 55 19 0 56 110 17 71 2.66e-17 77.4
Msa0276160 AT5G06800.2 41.964 112 57 3 28 134 169 277 2.85e-17 78.2
Msa0276160 AT5G06800.3 49.398 83 39 2 28 110 169 248 3.18e-17 77.0
Msa0276160 AT2G06020.1 52.542 59 28 0 49 107 81 139 3.57e-17 77.0
Msa0276160 AT3G24120.2 40.625 96 52 1 27 122 19 109 3.83e-17 77.0
Msa0276160 AT3G24120.1 40.625 96 52 1 27 122 19 109 4.07e-17 77.0
Msa0276160 AT4G13640.2 56.140 57 25 0 55 111 38 94 6.50e-17 76.3
Msa0276160 AT4G13640.1 56.140 57 25 0 55 111 38 94 6.56e-17 76.3
Msa0276160 AT1G79430.2 57.895 57 24 0 55 111 35 91 1.26e-16 76.3
Msa0276160 AT5G06800.1 49.398 83 39 2 28 110 169 248 1.42e-16 75.9
Msa0276160 AT1G69580.1 58.491 53 22 0 55 107 35 87 1.57e-15 72.8
Msa0276160 AT1G69580.2 58.491 53 22 0 55 107 35 87 1.68e-15 72.8
Msa0276160 AT3G04030.2 58.491 53 21 1 55 107 46 97 2.95e-15 72.4
Msa0276160 AT3G62670.1 31.250 160 91 6 1 145 159 314 1.53e-14 70.5
Msa0276160 AT2G03500.1 57.692 52 22 0 56 107 235 286 2.41e-13 67.0
Msa0276160 AT4G37180.1 50.847 59 29 0 49 107 205 263 3.99e-13 66.2
Msa0276160 AT3G10760.1 37.383 107 62 4 53 157 104 207 5.01e-13 65.9
Msa0276160 AT4G37180.2 50.847 59 29 0 49 107 212 270 5.35e-13 65.9
Msa0276160 AT5G59570.2 55.357 56 24 1 52 107 139 193 5.91e-13 65.5
Msa0276160 AT5G59570.1 55.357 56 24 1 52 107 139 193 5.91e-13 65.5
Msa0276160 AT1G25550.1 55.769 52 23 0 56 107 211 262 6.19e-13 65.5
Msa0276160 AT5G44190.1 46.269 67 35 1 53 119 146 211 6.22e-13 65.9
Msa0276160 AT5G44190.2 46.269 67 35 1 53 119 146 211 7.57e-13 65.5
Msa0276160 AT3G46640.2 54.545 55 24 1 53 107 143 196 2.60e-12 63.9
Msa0276160 AT3G46640.1 54.545 55 24 1 53 107 143 196 2.60e-12 63.9
Msa0276160 AT2G40970.1 37.895 95 54 2 53 143 104 197 3.21e-12 62.8
Msa0276160 AT3G46640.3 54.545 55 24 1 53 107 143 196 3.98e-12 63.2
Msa0276160 AT1G68670.1 53.846 52 24 0 56 107 217 268 1.14e-11 62.0
Msa0276160 AT4G37180.3 49.123 57 29 0 49 105 205 261 1.21e-11 61.6
Msa0276160 AT1G25550.2 51.923 52 25 0 56 107 211 262 1.38e-11 61.2
Msa0276160 AT5G05090.1 54.545 55 24 1 53 107 80 133 1.83e-11 60.8
Msa0276160 AT1G49190.1 33.654 104 59 3 51 144 212 315 2.43e-11 61.2
Msa0276160 AT1G49560.1 57.143 49 21 0 59 107 197 245 2.50e-11 60.8
Msa0276160 AT5G58080.1 43.243 74 39 2 53 126 193 263 2.58e-11 61.2
Msa0276160 AT3G16857.3 52.727 55 25 1 53 107 166 219 3.26e-11 60.8
Msa0276160 AT1G13300.1 55.102 49 22 0 59 107 186 234 4.30e-11 60.5
Msa0276160 AT3G16857.2 52.727 55 25 1 53 107 236 289 4.51e-11 60.5
Msa0276160 AT3G16857.1 52.727 55 25 1 53 107 236 289 4.62e-11 60.5

Find 37 sgRNAs with CRISPR-Local

Find 156 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTATTCCAGGGATGCTTAT+TGG 0.219949 2_3:+8385438 Msa0276160:intron
GTTTGGAGAATTACATTCTT+TGG 0.276827 2_3:-8385283 None:intergenic
ATGAATTGTAAAACTAACTT+TGG 0.405423 2_3:-8383685 None:intergenic
TCATGTCAAAAGCCACCTTC+AGG 0.411764 2_3:+8383732 Msa0276160:CDS
TTTACAATTCATGAATATCA+AGG 0.423774 2_3:+8383696 Msa0276160:CDS
TATTGGCTAGAGTCTTTCAA+TGG 0.428858 2_3:+8385455 Msa0276160:CDS
CAATGTTATTGCAGATGTAC+AGG 0.430914 2_3:+8383925 Msa0276160:intron
CAAAGTTGCCTCGGCTTCGT+TGG 0.452111 2_3:+8383372 Msa0276160:CDS
GTGCATGCAGTTCAAAGGCT+TGG 0.455826 2_3:+8383419 Msa0276160:CDS
AAACAAGAGCAGATGATTGA+AGG 0.472078 2_3:+8383954 Msa0276160:CDS
ACAAAGCAACGGTGAAGATC+TGG 0.473150 2_3:-8383398 None:intergenic
TTCTCCTACTAGCGGAGTTG+TGG 0.487718 2_3:+8383238 Msa0276160:CDS
GGCAACTTTGATCGAACATA+AGG 0.496488 2_3:-8383359 None:intergenic
CCAAGCTACTATCTGAATCA+TGG 0.514407 2_3:+8385260 Msa0276160:CDS
ATGTTCGATCAAAGTTGCCT+CGG 0.517554 2_3:+8383363 Msa0276160:CDS
TTTACCAGCTGCAAAGAGAA+AGG 0.520218 2_3:+8385187 Msa0276160:intron
TTACAATTCATGAATATCAA+GGG 0.527486 2_3:+8383697 Msa0276160:CDS
GCTTTGTGCATGCAGTTCAA+AGG 0.532535 2_3:+8383414 Msa0276160:CDS
CGCACATCAGAAGGTTGTGA+AGG 0.540162 2_3:-8383269 None:intergenic
CATGCAGTTCAAAGGCTTGG+AGG 0.546546 2_3:+8383422 Msa0276160:CDS
GAGTCTTTCAATGGAACTCA+AGG 0.550479 2_3:+8385464 Msa0276160:CDS
GCTTGGAACAAGGGCTGAGG+AGG 0.553779 2_3:-8385243 None:intergenic
CCATGATTCAGATAGTAGCT+TGG 0.557206 2_3:-8385260 None:intergenic
GTGTCCACAACTCCGCTAGT+AGG 0.568408 2_3:-8383242 None:intergenic
TCTTCTTCTTCTCCTACTAG+CGG 0.575646 2_3:+8383230 Msa0276160:CDS
TACAATTCATGAATATCAAG+GGG 0.575681 2_3:+8383698 Msa0276160:CDS
TGAAAAGTTCGCACATCAGA+AGG 0.577257 2_3:-8383278 None:intergenic
CAGATAGTAGCTTGGAACAA+GGG 0.609739 2_3:-8385252 None:intergenic
TCAGATAGTAGCTTGGAACA+AGG 0.613357 2_3:-8385253 None:intergenic
GTAGCTTGGAACAAGGGCTG+AGG 0.617170 2_3:-8385246 None:intergenic
GAACTGCATGCACAAAGCAA+CGG 0.628677 2_3:-8383409 None:intergenic
TCTGGTGTCCAACGAAGCCG+AGG 0.632658 2_3:-8383380 None:intergenic
CGAACATAAGGTCTCACCAC+AGG 0.634731 2_3:-8383347 None:intergenic
GCACATCAGAAGGTTGTGAA+GGG 0.634756 2_3:-8383268 None:intergenic
AGCTGCAAAGAGAAAGGACA+TGG 0.645199 2_3:+8385193 Msa0276160:intron
GATGAGTCTAAAAGCACCTG+TGG 0.662178 2_3:+8383331 Msa0276160:CDS
AGAGAAAGAGTACCTGAAGG+TGG 0.689810 2_3:-8383744 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTAGGAAAAAAAAAATT+TGG - chr2_3:8383895-8383914 None:intergenic 10.0%
!!! TAAATGTATATTTTTTTAAC+AGG - chr2_3:8385006-8385025 None:intergenic 10.0%
!! AATATTGAATAAAAGAACTA+GGG - chr2_3:8383464-8383483 None:intergenic 15.0%
!! TAATATTGAATAAAAGAACT+AGG - chr2_3:8383465-8383484 None:intergenic 15.0%
!!! ATGATTTTTTTAATAGTAGA+TGG - chr2_3:8383585-8383604 None:intergenic 15.0%
!!! TATTAATCAAACATTTTTAG+TGG - chr2_3:8383786-8383805 None:intergenic 15.0%
!! TAAAAAAACGAACATAAATA+AGG - chr2_3:8383847-8383866 None:intergenic 15.0%
!!! CATTGAAATTTCAAAATTTT+AGG - chr2_3:8383910-8383929 None:intergenic 15.0%
!!! AAGTAGAATTTTTTATTACA+GGG + chr2_3:8384400-8384419 Msa0276160:intron 15.0%
!! TTTATAAAAGTTTGTGTATT+TGG + chr2_3:8384503-8384522 Msa0276160:intron 15.0%
!! TATTTATATAAACTACTTTG+AGG + chr2_3:8385743-8385762 Msa0276160:intron 15.0%
!! TATGAAATAAACATGTAAAT+AGG - chr2_3:8385986-8386005 None:intergenic 15.0%
!! AAATAAAGGACTATTTAAAT+AGG - chr2_3:8386133-8386152 None:intergenic 15.0%
!!! TGACTTGAAAACATTATTTT+TGG + chr2_3:8383621-8383640 Msa0276160:intron 20.0%
!! ATGAATTGTAAAACTAACTT+TGG - chr2_3:8383688-8383707 None:intergenic 20.0%
!! TTTACAATTCATGAATATCA+AGG + chr2_3:8383696-8383715 Msa0276160:CDS 20.0%
!! TTACAATTCATGAATATCAA+GGG + chr2_3:8383697-8383716 Msa0276160:CDS 20.0%
!! GGTATTACTAATATTAATTG+TGG + chr2_3:8383975-8383994 Msa0276160:intron 20.0%
!! AATACATTAGACATATTGTT+TGG + chr2_3:8384085-8384104 Msa0276160:intron 20.0%
!! AATAGAACAAAACAAGAAAT+AGG + chr2_3:8384174-8384193 Msa0276160:intron 20.0%
!!! ACAAAATGATGTATTTTTCT+AGG - chr2_3:8384283-8384302 None:intergenic 20.0%
!!! AAAAATACATCATTTTGTGT+TGG + chr2_3:8384285-8384304 Msa0276160:intron 20.0%
!!! GAAGTAGAATTTTTTATTAC+AGG + chr2_3:8384399-8384418 Msa0276160:intron 20.0%
!! TCTAAATAGTGAAATTTGAT+TGG + chr2_3:8384681-8384700 Msa0276160:intron 20.0%
!! ACGATAAAAAAAAGAAAGTT+TGG - chr2_3:8385303-8385322 None:intergenic 20.0%
!!! CTTTACAATGCATATAATTT+TGG + chr2_3:8385402-8385421 Msa0276160:intron 20.0%
!! CCAAAAAAAAAACTTACAAA+AGG - chr2_3:8385928-8385947 None:intergenic 20.0%
!!! CCTTTTGTAAGTTTTTTTTT+TGG + chr2_3:8385925-8385944 Msa0276160:intron 20.0%
!!! AATCAAACTTTTAAATAGAC+TGG - chr2_3:8385952-8385971 None:intergenic 20.0%
!! ACTTATTTAAGTAAATAGCT+CGG - chr2_3:8386280-8386299 None:intergenic 20.0%
!! GTTATTAAATGATTGAATGT+TGG - chr2_3:8386392-8386411 None:intergenic 20.0%
!!! ATTTTGAATTGATTTTGTCT+TGG + chr2_3:8386449-8386468 Msa0276160:intron 20.0%
!! CTAGTGATAATTAATATGTA+TGG - chr2_3:8386513-8386532 None:intergenic 20.0%
! TCAAGTCAAATTAACAACAA+AGG - chr2_3:8383609-8383628 None:intergenic 25.0%
! TATATGTATGCTTGTATTAC+AGG + chr2_3:8383655-8383674 Msa0276160:intron 25.0%
! ATATGTATGCTTGTATTACA+GGG + chr2_3:8383656-8383675 Msa0276160:intron 25.0%
! TACAATTCATGAATATCAAG+GGG + chr2_3:8383698-8383717 Msa0276160:CDS 25.0%
! TCTTTGTAAATGGAAGAAAT+AGG + chr2_3:8384053-8384072 Msa0276160:intron 25.0%
!!! ATTTTTCTAGGAACAAAATC+TGG - chr2_3:8384271-8384290 None:intergenic 25.0%
! TTGAGGTCTATAATTTCTTT+GGG - chr2_3:8384362-8384381 None:intergenic 25.0%
! ATTGAGGTCTATAATTTCTT+TGG - chr2_3:8384363-8384382 None:intergenic 25.0%
!!! TACCACGTTTATTTTTATTG+AGG - chr2_3:8384379-8384398 None:intergenic 25.0%
!!! AGTTTTAAAAAAGTGTTCTG+AGG - chr2_3:8384442-8384461 None:intergenic 25.0%
! CATATTCAAAAAACTGTTTC+AGG - chr2_3:8384779-8384798 None:intergenic 25.0%
!! TATGTTCCTCAAACAATTTT+TGG + chr2_3:8384795-8384814 Msa0276160:intron 25.0%
! TAGATGATAACAGATAGATA+GGG - chr2_3:8384836-8384855 None:intergenic 25.0%
!! TACTTTATCAAGTCTTTTTG+TGG + chr2_3:8384897-8384916 Msa0276160:intron 25.0%
!! ATACTTTATCAAGTCTTATC+CGG + chr2_3:8384926-8384945 Msa0276160:intron 25.0%
! TTGAATCACATATCATAATG+TGG - chr2_3:8385054-8385073 None:intergenic 25.0%
! CATTATGATATGTGATTCAA+TGG + chr2_3:8385054-8385073 Msa0276160:intron 25.0%
!!! TTCCTCTCTTCTTTTATTTA+CGG + chr2_3:8385322-8385341 Msa0276160:intron 25.0%
! ATTTCTCTCTTAATTATTCC+AGG + chr2_3:8385426-8385445 Msa0276160:intron 25.0%
! TTTCTCTCTTAATTATTCCA+GGG + chr2_3:8385427-8385446 Msa0276160:intron 25.0%
! CAATCAATTGTTATCTGTAA+TGG + chr2_3:8385491-8385510 Msa0276160:intron 25.0%
! CATAGTATTAGTATGTAGAA+AGG - chr2_3:8385712-8385731 None:intergenic 25.0%
! AAAAAAATGTGTTTGTGTGT+TGG - chr2_3:8385793-8385812 None:intergenic 25.0%
!!! AAGACTTGCAAATTTTTTGT+AGG - chr2_3:8385891-8385910 None:intergenic 25.0%
! GACTATTTAAATAGGCTTTA+AGG - chr2_3:8386125-8386144 None:intergenic 25.0%
! TCAAGTAACAACTCAAATAA+AGG - chr2_3:8386147-8386166 None:intergenic 25.0%
! CTTATTTAAGTAAATAGCTC+GGG - chr2_3:8386279-8386298 None:intergenic 25.0%
!! AATGATTGAATGTTGGTTAA+GGG - chr2_3:8386385-8386404 None:intergenic 25.0%
!! AAATGATTGAATGTTGGTTA+AGG - chr2_3:8386386-8386405 None:intergenic 25.0%
TATGTATGCTTGTATTACAG+GGG + chr2_3:8383657-8383676 Msa0276160:intron 30.0%
AAATGCATGTTATCATGTCA+CGG + chr2_3:8384015-8384034 Msa0276160:intron 30.0%
CATGTATGCATCTTTGTAAA+TGG + chr2_3:8384043-8384062 Msa0276160:intron 30.0%
CATATTGTTTGGTACAATAG+AGG + chr2_3:8384096-8384115 Msa0276160:intron 30.0%
GACCTCAATAAAAATAAACG+TGG + chr2_3:8384374-8384393 Msa0276160:intron 30.0%
!! AGTTTGTGTATTTGGTGTAT+CGG + chr2_3:8384511-8384530 Msa0276160:intron 30.0%
ATAACAGATAGATAGGGAAT+AGG - chr2_3:8384830-8384849 None:intergenic 30.0%
CTAGATGATAACAGATAGAT+AGG - chr2_3:8384837-8384856 None:intergenic 30.0%
GTTTGGAGAATTACATTCTT+TGG - chr2_3:8385286-8385305 None:intergenic 30.0%
TTGTTGGTCAACAAAATTGT+AGG - chr2_3:8385833-8385852 None:intergenic 30.0%
! CAATTTTGTTGACCAACAAT+AGG + chr2_3:8385834-8385853 Msa0276160:intron 30.0%
!!! TTGCAAATTTTTTGTAGGTC+AGG - chr2_3:8385886-8385905 None:intergenic 30.0%
AAACATGTAAATAGGCTTTC+AGG - chr2_3:8385978-8385997 None:intergenic 30.0%
TGAAATAGACTAAGGTCTTA+CGG - chr2_3:8386094-8386113 None:intergenic 30.0%
CCTTAAAGTGAAATAGACTA+AGG - chr2_3:8386102-8386121 None:intergenic 30.0%
CCTTAGTCTATTTCACTTTA+AGG + chr2_3:8386099-8386118 Msa0276160:intron 30.0%
! ACCTTGCCTTATTAATAAAC+AGG + chr2_3:8386319-8386338 Msa0276160:intron 30.0%
!! TTGAATTGATTTTGTCTTGG+AGG + chr2_3:8386452-8386471 Msa0276160:intron 30.0%
!! TAAGGTAATTAGCTTGCAAG+AGG - chr2_3:8383829-8383848 None:intergenic 35.0%
CAATGTTATTGCAGATGTAC+AGG + chr2_3:8383925-8383944 Msa0276160:intron 35.0%
AAACAAGAGCAGATGATTGA+AGG + chr2_3:8383954-8383973 Msa0276160:CDS 35.0%
! ACTTTTCTCTATCTTGGTCA+CGG - chr2_3:8384232-8384251 None:intergenic 35.0%
!!! GACGTTACTTTTCTCTATCT+TGG - chr2_3:8384238-8384257 None:intergenic 35.0%
!! GTTTGTGTATTTGGTGTATC+GGG + chr2_3:8384512-8384531 Msa0276160:intron 35.0%
AAGTCGCCAAAAATTGTTTG+AGG - chr2_3:8384804-8384823 None:intergenic 35.0%
!! TCTTTTTGCTATGTGACTGA+TGG + chr2_3:8384865-8384884 Msa0276160:intron 35.0%
CACCGTAAATAAAAGAAGAG+AGG - chr2_3:8385327-8385346 None:intergenic 35.0%
!! CTCTCTTCTTTTATTTACGG+TGG + chr2_3:8385325-8385344 Msa0276160:intron 35.0%
ATTATTCCAGGGATGCTTAT+TGG + chr2_3:8385438-8385457 Msa0276160:intron 35.0%
! TATTGGCTAGAGTCTTTCAA+TGG + chr2_3:8385455-8385474 Msa0276160:CDS 35.0%
ATTTACGTACTTGAACAGTC+TGG - chr2_3:8385668-8385687 None:intergenic 35.0%
GTGATAGAAGAAGTGGAATT+GGG - chr2_3:8385771-8385790 None:intergenic 35.0%
CCTTCGTGAAATAGACTTTA+AGG - chr2_3:8386060-8386079 None:intergenic 35.0%
CCTTAAAGTCTATTTCACGA+AGG + chr2_3:8386057-8386076 Msa0276160:intron 35.0%
AAAGCATACGAAGCTTGATA+CGG - chr2_3:8386242-8386261 None:intergenic 35.0%
GCCTGTTTATTAATAAGGCA+AGG - chr2_3:8386323-8386342 None:intergenic 35.0%
GGCTTGCCTGTTTATTAATA+AGG - chr2_3:8386328-8386347 None:intergenic 35.0%
TAATGTGTGTTGTGTTAGTG+TGG + chr2_3:8386556-8386575 Msa0276160:intron 35.0%
TCTTCTTCTTCTCCTACTAG+CGG + chr2_3:8383230-8383249 Msa0276160:CDS 40.0%
TGAAAAGTTCGCACATCAGA+AGG - chr2_3:8383281-8383300 None:intergenic 40.0%
GGCAACTTTGATCGAACATA+AGG - chr2_3:8383362-8383381 None:intergenic 40.0%
ATGTTCGATCAAAGTTGCCT+CGG + chr2_3:8383363-8383382 Msa0276160:CDS 40.0%
! GTGGCTTTTGACATGAGATA+TGG - chr2_3:8383728-8383747 None:intergenic 40.0%
!! TGAAGAGAAAGAGTACCTGA+AGG - chr2_3:8383750-8383769 None:intergenic 40.0%
TAGGTGTACGTAAGCATAAC+TGG + chr2_3:8384193-8384212 Msa0276160:intron 40.0%
AAGCAAATCCTATCAGCACA+AGG - chr2_3:8384956-8384975 None:intergenic 40.0%
TTTACCAGCTGCAAAGAGAA+AGG + chr2_3:8385187-8385206 Msa0276160:intron 40.0%
CAGATAGTAGCTTGGAACAA+GGG - chr2_3:8385255-8385274 None:intergenic 40.0%
TCAGATAGTAGCTTGGAACA+AGG - chr2_3:8385256-8385275 None:intergenic 40.0%
CCATGATTCAGATAGTAGCT+TGG - chr2_3:8385263-8385282 None:intergenic 40.0%
CCAAGCTACTATCTGAATCA+TGG + chr2_3:8385260-8385279 Msa0276160:CDS 40.0%
GAGTCTTTCAATGGAACTCA+AGG + chr2_3:8385464-8385483 Msa0276160:CDS 40.0%
GAAAGGAGAGACTAATTGAG+AGG - chr2_3:8385695-8385714 None:intergenic 40.0%
GGTGATAGAAGAAGTGGAAT+TGG - chr2_3:8385772-8385791 None:intergenic 40.0%
!! TGTGTTGGTGATAGAAGAAG+TGG - chr2_3:8385778-8385797 None:intergenic 40.0%
! ATTGAATGTTGGTTAAGGGC+GGG - chr2_3:8386381-8386400 None:intergenic 40.0%
! GATTGAATGTTGGTTAAGGG+CGG - chr2_3:8386382-8386401 None:intergenic 40.0%
GCACATCAGAAGGTTGTGAA+GGG - chr2_3:8383271-8383290 None:intergenic 45.0%
! GGTGCTTTTAGACTCATCTC+TGG - chr2_3:8383329-8383348 None:intergenic 45.0%
!! GATGAGTCTAAAAGCACCTG+TGG + chr2_3:8383331-8383350 Msa0276160:CDS 45.0%
ACAAAGCAACGGTGAAGATC+TGG - chr2_3:8383401-8383420 None:intergenic 45.0%
GAACTGCATGCACAAAGCAA+CGG - chr2_3:8383412-8383431 None:intergenic 45.0%
GCTTTGTGCATGCAGTTCAA+AGG + chr2_3:8383414-8383433 Msa0276160:CDS 45.0%
TCATGTCAAAAGCCACCTTC+AGG + chr2_3:8383732-8383751 Msa0276160:CDS 45.0%
!! AGAGAAAGAGTACCTGAAGG+TGG - chr2_3:8383747-8383766 None:intergenic 45.0%
CTTGCAAGAGGTGGAAAGAA+TGG - chr2_3:8383817-8383836 None:intergenic 45.0%
!! GGTAATTAGCTTGCAAGAGG+TGG - chr2_3:8383826-8383845 None:intergenic 45.0%
ATGTCCTTTCTCTTTGCAGC+TGG - chr2_3:8385194-8385213 None:intergenic 45.0%
AGCTGCAAAGAGAAAGGACA+TGG + chr2_3:8385193-8385212 Msa0276160:intron 45.0%
AGTCGGATCTGACCTATTGT+TGG - chr2_3:8385849-8385868 None:intergenic 45.0%
AGGTCAGATCCGACTTTGAT+AGG + chr2_3:8385854-8385873 Msa0276160:intron 45.0%
AGGCTCAGACCTATCAAAGT+CGG - chr2_3:8385866-8385885 None:intergenic 45.0%
GACTAAGGTCTTACGGTGAT+AGG - chr2_3:8386087-8386106 None:intergenic 45.0%
TAGGTCATACGGGCCTATAA+GGG - chr2_3:8386356-8386375 None:intergenic 45.0%
ATAGGTCATACGGGCCTATA+AGG - chr2_3:8386357-8386376 None:intergenic 45.0%
!! AGAAATTTAAATTAAAAAAT+AGG - chr2_3:8383499-8383518 None:intergenic 5.0%
TTCTCCTACTAGCGGAGTTG+TGG + chr2_3:8383238-8383257 Msa0276160:CDS 50.0%
CGCACATCAGAAGGTTGTGA+AGG - chr2_3:8383272-8383291 None:intergenic 50.0%
CGAACATAAGGTCTCACCAC+AGG - chr2_3:8383350-8383369 None:intergenic 50.0%
GTGCATGCAGTTCAAAGGCT+TGG + chr2_3:8383419-8383438 Msa0276160:CDS 50.0%
CATGCAGTTCAAAGGCTTGG+AGG + chr2_3:8383422-8383441 Msa0276160:CDS 50.0%
CCTATCAGCACAAGGTGTAC+CGG - chr2_3:8384948-8384967 None:intergenic 50.0%
CTCTAGCCAATAAGCATCCC+TGG - chr2_3:8385447-8385466 None:intergenic 50.0%
TACGGGCCTATAAGGGTCTA+CGG - chr2_3:8386349-8386368 None:intergenic 50.0%
AAGGGCGGGAATAGGTCATA+CGG - chr2_3:8386367-8386386 None:intergenic 50.0%
! TGTTGGTTAAGGGCGGGAAT+AGG - chr2_3:8386375-8386394 None:intergenic 50.0%
GTGTCCACAACTCCGCTAGT+AGG - chr2_3:8383245-8383264 None:intergenic 55.0%
CAAAGTTGCCTCGGCTTCGT+TGG + chr2_3:8383372-8383391 Msa0276160:CDS 55.0%
! CCGGTACACCTTGTGCTGAT+AGG + chr2_3:8384945-8384964 Msa0276160:intron 55.0%
GTAGCTTGGAACAAGGGCTG+AGG - chr2_3:8385249-8385268 None:intergenic 55.0%
GGCAAGCCGTAGACCCTTAT+AGG + chr2_3:8386340-8386359 Msa0276160:intron 55.0%
AGGGCGGGAATAGGTCATAC+GGG - chr2_3:8386366-8386385 None:intergenic 55.0%
TCTGGTGTCCAACGAAGCCG+AGG - chr2_3:8383383-8383402 None:intergenic 60.0%
GCTTGGAACAAGGGCTGAGG+AGG - chr2_3:8385246-8385265 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 8383218 8386584 8383218 ID=Msa0276160;Name=Msa0276160
chr2_3 mRNA 8383218 8386584 8383218 ID=Msa0276160-mRNA-1;Parent=Msa0276160;Name=Msa0276160-mRNA-1;_AED=0.20;_eAED=0.20;_QI=0|0.8|0.5|0.83|0.8|0.83|6|0|164
chr2_3 exon 8383218 8383452 8383218 ID=Msa0276160-mRNA-1:exon:3766;Parent=Msa0276160-mRNA-1
chr2_3 exon 8383677 8383753 8383677 ID=Msa0276160-mRNA-1:exon:3767;Parent=Msa0276160-mRNA-1
chr2_3 exon 8383939 8383975 8383939 ID=Msa0276160-mRNA-1:exon:3768;Parent=Msa0276160-mRNA-1
chr2_3 exon 8385195 8385291 8385195 ID=Msa0276160-mRNA-1:exon:3769;Parent=Msa0276160-mRNA-1
chr2_3 exon 8385448 8385485 8385448 ID=Msa0276160-mRNA-1:exon:3770;Parent=Msa0276160-mRNA-1
chr2_3 exon 8386574 8386584 8386574 ID=Msa0276160-mRNA-1:exon:3771;Parent=Msa0276160-mRNA-1
chr2_3 CDS 8383218 8383452 8383218 ID=Msa0276160-mRNA-1:cds;Parent=Msa0276160-mRNA-1
chr2_3 CDS 8383677 8383753 8383677 ID=Msa0276160-mRNA-1:cds;Parent=Msa0276160-mRNA-1
chr2_3 CDS 8383939 8383975 8383939 ID=Msa0276160-mRNA-1:cds;Parent=Msa0276160-mRNA-1
chr2_3 CDS 8385195 8385291 8385195 ID=Msa0276160-mRNA-1:cds;Parent=Msa0276160-mRNA-1
chr2_3 CDS 8385448 8385485 8385448 ID=Msa0276160-mRNA-1:cds;Parent=Msa0276160-mRNA-1
chr2_3 CDS 8386574 8386584 8386574 ID=Msa0276160-mRNA-1:cds;Parent=Msa0276160-mRNA-1
Gene Sequence

>Msa0276160

ATGTCTTCATCTTCTTCTTCTTCTCCTACTAGCGGAGTTGTGGACACTAACCCTTCACAACCTTCTGATGTGCGAACTTTTCACAGTCGTTTAGATTCTCATCAGACTCCAGAGATGAGTCTAAAAGCACCTGTGGTGAGACCTTATGTTCGATCAAAGTTGCCTCGGCTTCGTTGGACACCAGATCTTCACCGTTGCTTTGTGCATGCAGTTCAAAGGCTTGGAGGAGAAGAGAGGGCTACACCAAAGTTAGTTTTACAATTCATGAATATCAAGGGGCTTACCATATCTCATGTCAAAAGCCACCTTCAGATGTACAGGAGCATGAAACAAGAGCAGATGATTGAAGCTGCAAAGAGAAAGGACATGGCAGCTGATTTATCAGTTTCAGCAATCTCCTCCTCAGCCCTTGTTCCAAGCTACTATCTGAATCATGGCCAAAGAATGGATGCTTATTGGCTAGAGTCTTTCAATGGAACTCAAGTGTGGTGTTGA
Protein sequence

>Msa0276160

MSSSSSSSPTSGVVDTNPSQPSDVRTFHSRLDSHQTPEMSLKAPVVRPYVRSKLPRLRWTPDLHRCFVHAVQRLGGEERATPKLVLQFMNIKGLTISHVKSHLQMYRSMKQEQMIEAAKRKDMAADLSVSAISSSALVPSYYLNHGQRMDAYWLESFNGTQVWC*