AlfalfaGEDB | Msa0277540

M. sativa cultivar zhongmu-4 / Msa0277540

KEGG: K15032
GO: -
PFAM: PF02536.17

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0277540	XP_003593962.1	83.422	374	53	2	1	373	1	366	0.0	613

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0277540	G7IPZ5	83.422	374	53	2	1	373	1	366	0.0	613

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TFs/TRs:

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Gene ID	Type	Classification
Msa0277540	TR	mTERF

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0277540	MtrunA17_Chr2g0286191	83.422	374	53	2	1	373	1	366	0.0	613
Msa0277540	MtrunA17_Chr2g0295761	48.406	345	163	5	30	366	52	389	1.10e-103	311
Msa0277540	MtrunA17_Chr2g0295731	46.018	339	177	4	32	366	41	377	3.37e-99	299
Msa0277540	MtrunA17_Chr4g0067121	46.588	337	175	4	33	366	46	380	1.79e-97	295
Msa0277540	MtrunA17_Chr2g0295741	45.706	361	184	6	12	366	22	376	1.24e-96	293
Msa0277540	MtrunA17_Chr2g0296151	44.474	371	199	5	1	366	2	370	1.44e-94	287
Msa0277540	MtrunA17_Chr2g0296121	45.028	362	193	4	9	366	97	456	7.56e-94	288
Msa0277540	MtrunA17_Chr2g0296111	45.699	372	180	7	6	366	10	370	1.25e-90	277
Msa0277540	MtrunA17_Chr2g0296301	42.450	351	199	3	18	365	15	365	1.93e-89	275
Msa0277540	MtrunA17_Chr2g0286221	76.074	163	39	0	208	370	11	173	2.10e-86	259

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0277540	AT5G07900.1	38.024	334	201	4	36	363	66	399	3.10e-75	239
Msa0277540	AT1G21150.1	34.821	336	213	3	38	367	55	390	1.27e-59	197
Msa0277540	AT1G21150.2	34.821	336	213	3	38	367	119	454	8.71e-59	197
Msa0277540	AT1G21150.3	34.030	335	215	3	39	367	20	354	8.39e-58	192
Msa0277540	AT1G61980.3	29.178	377	208	11	36	363	49	415	1.04e-41	151
Msa0277540	AT1G61980.2	29.178	377	208	11	36	363	49	415	1.04e-41	151
Msa0277540	AT1G61980.1	29.178	377	208	11	36	363	49	415	1.04e-41	151
Msa0277540	AT1G62120.1	27.604	384	227	11	30	367	47	425	3.03e-40	147
Msa0277540	AT1G61970.2	27.513	378	213	10	36	363	49	415	1.12e-38	143
Msa0277540	AT1G61970.7	27.513	378	213	10	36	363	49	415	1.12e-38	143

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Find 55 sgRNAs with CRISPR-Local

Find 172 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTCAGCATAAGAAGTAT+TGG	0.453268	2_3:-10128149	None:intergenic
AAAGAAGGAAATTGAATTTA+GGG	0.185509	2_3:-10127572	None:intergenic
AACTACCTCATTCAAAATTG+TGG	0.437653	2_3:+10127375	Msa0277540:CDS
AAGAAGATGAAGAGTTATTG+AGG	0.449182	2_3:-10127304	None:intergenic
AAGCTTTGAGACAACATTAG+AGG	0.354334	2_3:-10127642	None:intergenic
AATAAGGCGATGACTGAATC+AGG	0.360376	2_3:-10127459	None:intergenic
AATACTTCTTATGCTGAGTT+TGG	0.261332	2_3:+10128151	Msa0277540:CDS
AATTTCAACGCAAAAGCCCT+AGG	0.586081	2_3:-10128188	None:intergenic
ACAGATGCCAACATACACCA+TGG	0.670800	2_3:-10128053	None:intergenic
ACTACCTCATTCAAAATTGT+GGG	0.457092	2_3:+10127376	Msa0277540:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGAAGATGAAGAGTTATTG+AGG	-	chr2_3:10127307-10127326	None:intergenic	30.0%
AACTACCTCATTCAAAATTG+TGG	+	chr2_3:10127375-10127394	Msa0277540:CDS	30.0%
ACTACCTCATTCAAAATTGT+GGG	+	chr2_3:10127376-10127395	Msa0277540:CDS	30.0%
GAAATTGAATTTAGGGAGAA+TGG	-	chr2_3:10127568-10127587	None:intergenic	30.0%
ATTAATCTTCCTTTCCCATA+CGG	-	chr2_3:10127976-10127995	None:intergenic	30.0%
AATACTTCTTATGCTGAGTT+TGG	+	chr2_3:10128151-10128170	Msa0277540:CDS	30.0%
TTATGCTGAGTTTGGAAAAA+AGG	+	chr2_3:10128159-10128178	Msa0277540:CDS	30.0%
TATGCTGAGTTTGGAAAAAA+GGG	+	chr2_3:10128160-10128179	Msa0277540:CDS	30.0%
TCAATCCAAAGGTTTGATTA+AGG	+	chr2_3:10128214-10128233	Msa0277540:CDS	30.0%
AAGAAGATGAAGAGTTATTG+AGG	-	chr2_3:10127307-10127326	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_3	gene	10127249	10128370	10127249	ID=Msa0277540;Name=Msa0277540
chr2_3	mRNA	10127249	10128370	10127249	ID=Msa0277540-mRNA-1;Parent=Msa0277540;Name=Msa0277540-mRNA-1;_AED=0.31;_eAED=0.31;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|373
chr2_3	exon	10127249	10128370	10127249	ID=Msa0277540-mRNA-1:exon:4506;Parent=Msa0277540-mRNA-1
chr2_3	CDS	10127249	10128370	10127249	ID=Msa0277540-mRNA-1:cds;Parent=Msa0277540-mRNA-1

Gene Sequence

>Msa0277540

ATGCTTTGTTACTTTCGTAGACCATTACTCTGCCCTAACCCCCTCTCATTCAAAACCTCAATAACTCTTCATCTTCTTCAACTCTCTTCTTCTTCTTCTTCTTCCCAAAATCACTCTTTTACAGTCAACTACCTCATTCAAAATTGTGGGTTTTCACCAGAAACTGCTTCTAAACTCTCTAAACGAGTTCATCTCAACAACTCACAAAAACCTGATTCAGTCATCGCCTTATTCAAATCATATGGTTTCTCTAATTCCCAATTATCATCTCTCATCAAAACTCGCACGGATATTCTCTCTTATGATCCCAACAAAACCATTCTCCCTAAATTCAATTTCCTTCTTTCTAAGGGTGCTTCAAATTCAGATCTTGTTCACATCATCACCAGAAATCCTCTAATGTTGTCTCAAAGCTTGGAAAATACAATAACCCCTTGTTATCATTTTATCAAAAGATTCTTACTTTCTGATCAATCTACCATTGCAGCTCTCAAACATTGCTCTTGTTTCCTTTATTCTAAATTCCCATCTCAAAATATTCAATCCCTGCTTCAAAATGGGGTTCCTGAATCCAAAATTGTTTTTTTGTTTCGAAATTGGTATAACATGCTCTCTAAGAAACCTGCAGTATTTGAGAAGGCAGTTTTGGAAGTAAAACAATTAGGATTCAATCCTAACAATACGTTTTTCCTTATCGCGCTGCATGCTAAGATTAGTCCAAAATCCGTATGGGAAAGGAAGATTAATGCGTATAAGAAATGGGGTTGGTCTGATGAAGTTATTGTCTCAGCATTTTTGAAGTATCCATGGTGTATGTTGGCATCTGTGGAAAAAATTGAAGCAGTGATGCAGTTTTTTGTCAATCACATGGGTTGGGAGTCTAATGTGCTTGCCAAATATCCAATACTTCTTATGCTGAGTTTGGAAAAAAGGGTTATTCCTAGGGCTTTTGCGTTGAAATTTCTTCAATCCAAAGGTTTGATTAAGGATGCTAAGTCCCCTACACCTTTCAAAGTCTCTGAGGATCTGTTTTTGAAGAGATATGTGAATTGCTACGAGGAAGAAGCATCTCAGCTGTTGAAATTGTATGAAGAGAAAAGAGATGCTTCAACTTTCAAATAA

Protein sequence

>Msa0277540

MLCYFRRPLLCPNPLSFKTSITLHLLQLSSSSSSSQNHSFTVNYLIQNCGFSPETASKLSKRVHLNNSQKPDSVIALFKSYGFSNSQLSSLIKTRTDILSYDPNKTILPKFNFLLSKGASNSDLVHIITRNPLMLSQSLENTITPCYHFIKRFLLSDQSTIAALKHCSCFLYSKFPSQNIQSLLQNGVPESKIVFLFRNWYNMLSKKPAVFEKAVLEVKQLGFNPNNTFFLIALHAKISPKSVWERKINAYKKWGWSDEVIVSAFLKYPWCMLASVEKIEAVMQFFVNHMGWESNVLAKYPILLMLSLEKRVIPRAFALKFLQSKGLIKDAKSPTPFKVSEDLFLKRYVNCYEEEASQLLKLYEEKRDASTFK*