AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0280270

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0280270 MtrunA17_Chr6g0470151 42.005 369 161 9 1 332 1 353 6.60e-55 187
Msa0280270 MtrunA17_Chr8g0366921 42.121 330 141 9 1 290 1 320 1.01e-50 175
Msa0280270 MtrunA17_Chr2g0289181 41.631 233 87 3 296 479 1 233 1.05e-50 172
Msa0280270 MtrunA17_Chr2g0293901 68.235 170 30 2 1 146 1 170 2.38e-49 167
Msa0280270 MtrunA17_Chr7g0266621 45.860 314 132 5 1 288 1 302 5.12e-44 157
Msa0280270 MtrunA17_Chr8g0368721 46.708 319 134 6 1 290 1 312 5.68e-42 151
Msa0280270 MtrunA17_Chr1g0202461 51.579 95 44 2 275 369 229 321 1.94e-24 104
Msa0280270 MtrunA17_Chr8g0348311 53.571 84 37 1 286 369 134 215 2.17e-22 98.2
Msa0280270 MtrunA17_Chr7g0248691 51.163 86 40 1 284 369 131 214 5.52e-21 94.0
Msa0280270 MtrunA17_Chr4g0053281 43.519 108 54 1 266 366 216 323 8.13e-20 90.5
Msa0280270 MtrunA17_Chr5g0401821 46.988 83 41 1 287 366 174 256 1.79e-19 88.2
Msa0280270 MtrunA17_Chr7g0270051 41.026 117 56 2 260 369 3 113 2.64e-19 87.8
Msa0280270 MtrunA17_Chr8g0379461 55.738 61 27 0 309 369 76 136 6.55e-19 85.9
Msa0280270 MtrunA17_Chr8g0387501 41.071 112 47 2 310 402 130 241 6.98e-19 86.7
Msa0280270 MtrunA17_Chr2g0293481 42.056 107 51 2 274 369 221 327 8.51e-19 89.0
Msa0280270 MtrunA17_Chr4g0071521 44.578 83 43 1 287 366 220 302 1.38e-18 86.7
Msa0280270 MtrunA17_Chr2g0284321 43.617 94 48 2 281 369 2 95 2.55e-18 83.6
Msa0280270 MtrunA17_Chr2g0319961 57.377 61 26 0 309 369 80 140 2.89e-18 84.7
Msa0280270 MtrunA17_Chr1g0184691 57.627 59 25 0 310 368 294 352 5.77e-18 85.5
Msa0280270 MtrunA17_Chr1g0147881 42.683 82 44 1 288 366 238 319 3.31e-17 82.8
Msa0280270 MtrunA17_Chr7g0228941 49.231 65 33 0 303 367 46 110 1.00e-16 76.6
Msa0280270 MtrunA17_Chr4g0074001 46.250 80 41 2 291 369 340 418 3.73e-16 80.9
Msa0280270 MtrunA17_Chr7g0263531 55.072 69 29 2 305 373 185 251 6.15e-16 79.7
Msa0280270 MtrunA17_Chr7g0263531 43.750 64 35 1 306 369 358 420 9.06e-14 73.2
Msa0280270 MtrunA17_Chr3g0139021 39.080 87 52 1 281 366 285 371 7.14e-16 79.3
Msa0280270 MtrunA17_Chr6g0470161 57.143 56 24 0 424 479 65 120 1.22e-15 73.2
Msa0280270 MtrunA17_Chr6g0470161 39.024 82 48 1 340 421 35 114 4.69e-11 60.1
Msa0280270 MtrunA17_Chr7g0218081 50.000 66 32 1 304 369 405 469 3.45e-15 77.8
Msa0280270 MtrunA17_Chr7g0218081 46.970 66 33 2 305 370 224 287 3.49e-13 71.6
Msa0280270 MtrunA17_Chr5g0445311 43.056 72 40 1 298 369 353 423 7.80e-15 77.0
Msa0280270 MtrunA17_Chr5g0445311 49.206 63 30 2 308 370 199 259 1.58e-11 66.6
Msa0280270 MtrunA17_Chr2g0302391 46.154 65 35 0 309 373 211 275 1.67e-14 75.5
Msa0280270 MtrunA17_Chr3g0101131 41.667 72 41 1 298 369 404 474 3.65e-14 75.1
Msa0280270 MtrunA17_Chr3g0101131 46.479 71 36 2 305 375 239 307 1.25e-12 70.1
Msa0280270 MtrunA17_Chr5g0447701 48.485 66 33 1 304 369 247 311 3.69e-14 73.9
Msa0280270 MtrunA17_Chr3g0099641 33.333 120 69 2 250 369 203 311 4.19e-14 74.3
Msa0280270 MtrunA17_Chr4g0075201 28.000 175 107 4 211 369 156 327 4.98e-14 74.3
Msa0280270 MtrunA17_Chr5g0418191 45.455 66 35 1 304 369 315 379 1.43e-13 72.4
Msa0280270 MtrunA17_Chr5g0418191 43.678 87 42 4 305 387 147 230 5.51e-12 67.8
Msa0280270 MtrunA17_Chr7g0270191 36.170 94 56 3 279 369 112 204 1.82e-13 70.9
Msa0280270 MtrunA17_Chr5g0432161 48.333 60 30 1 310 369 422 480 1.98e-13 72.4
Msa0280270 MtrunA17_Chr5g0432161 52.381 63 28 2 308 370 243 303 9.58e-13 70.5
Msa0280270 MtrunA17_Chr4g0001361 43.750 64 36 0 310 373 163 226 2.12e-13 71.2
Msa0280270 MtrunA17_Chr1g0194481 40.741 81 46 2 289 369 139 217 2.30e-13 71.2
Msa0280270 MtrunA17_Chr1g0150271 29.012 162 98 4 215 371 14 163 2.34e-13 70.1
Msa0280270 MtrunA17_Chr7g0249311 30.597 134 76 5 267 385 336 467 4.16e-13 71.6
Msa0280270 MtrunA17_Chr7g0249311 45.714 70 36 2 308 377 178 245 5.26e-11 65.1
Msa0280270 MtrunA17_Chr7g0270831 38.462 78 47 1 292 369 200 276 5.64e-13 70.9
Msa0280270 MtrunA17_Chr2g0322391 37.234 94 54 2 276 369 321 409 6.45e-13 70.9
Msa0280270 MtrunA17_Chr3g0106491 46.667 60 31 1 310 369 366 424 8.73e-13 70.5
Msa0280270 MtrunA17_Chr3g0106491 51.613 62 28 2 308 369 197 256 2.73e-12 68.9
Msa0280270 MtrunA17_Chr3g0136961 43.860 57 32 0 310 366 139 195 9.42e-13 68.9
Msa0280270 MtrunA17_Chr2g0288871 35.000 100 61 3 271 369 269 365 1.14e-12 70.1
Msa0280270 MtrunA17_Chr4g0042831 43.750 64 35 1 306 369 275 337 1.25e-12 69.3
Msa0280270 MtrunA17_Chr3g0079691 40.404 99 40 4 308 405 279 359 1.39e-12 70.1
Msa0280270 MtrunA17_Chr3g0079691 37.975 79 48 1 310 388 496 573 2.94e-12 68.9
Msa0280270 MtrunA17_Chr4g0051881 37.000 100 60 3 305 403 175 272 1.46e-12 68.9
Msa0280270 MtrunA17_Chr7g0249301 47.541 61 29 3 310 369 293 351 2.38e-12 68.6
Msa0280270 MtrunA17_Chr7g0243891 31.884 138 66 4 301 412 208 343 3.80e-12 68.6
Msa0280270 MtrunA17_Chr1g0150281 43.860 57 32 0 310 366 141 197 3.90e-12 67.0
Msa0280270 MtrunA17_Chr4g0068541 38.961 77 44 2 310 384 209 284 4.11e-12 67.8
Msa0280270 MtrunA17_Chr4g0025061 35.616 73 47 0 304 376 355 427 4.66e-12 68.2
Msa0280270 MtrunA17_Chr1g0150801 45.902 61 32 1 306 366 99 158 6.21e-12 63.9
Msa0280270 MtrunA17_Chr4g0060271 39.744 78 45 1 294 369 238 315 6.76e-12 67.8
Msa0280270 MtrunA17_Chr3g0129321 42.188 64 36 1 306 369 115 177 7.58e-12 64.7
Msa0280270 MtrunA17_Chr2g0294231 46.667 60 31 1 310 369 204 262 8.06e-12 66.6
Msa0280270 MtrunA17_Chr2g0333851 39.024 82 46 1 294 371 150 231 9.13e-12 66.2
Msa0280270 MtrunA17_Chr4g0004911 45.000 60 32 1 310 369 337 395 9.42e-12 67.0
Msa0280270 MtrunA17_Chr4g0051181 29.851 134 79 2 236 369 185 303 1.13e-11 67.0
Msa0280270 MtrunA17_Chr7g0216891 32.743 113 71 3 254 366 169 276 1.32e-11 67.0
Msa0280270 MtrunA17_Chr5g0443131 43.077 65 36 1 310 374 158 221 1.37e-11 65.5
Msa0280270 MtrunA17_Chr4g0025051 35.616 73 47 0 304 376 330 402 1.78e-11 66.6
Msa0280270 MtrunA17_Chr7g0238921 42.254 71 38 2 296 366 113 180 1.96e-11 65.1
Msa0280270 MtrunA17_Chr8g0383251 38.554 83 48 2 289 369 86 167 2.72e-11 62.4
Msa0280270 MtrunA17_Chr5g0418781 35.000 100 63 2 267 366 83 180 3.35e-11 63.9
Msa0280270 MtrunA17_Chr1g0188941 32.184 87 54 2 283 369 29 110 8.64e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0280270 AT5G52830.1 53.571 84 38 1 286 369 143 225 5.66e-23 100
Msa0280270 AT4G01250.1 40.000 120 68 2 250 369 73 188 1.07e-21 95.5
Msa0280270 AT5G28650.1 48.235 85 41 1 287 368 237 321 1.35e-18 87.0
Msa0280270 AT4G31550.2 46.341 82 41 1 288 366 221 302 1.37e-18 87.0
Msa0280270 AT4G31550.1 55.738 61 27 0 306 366 243 303 1.61e-18 87.0
Msa0280270 AT2G23320.1 59.649 57 23 0 310 366 241 297 1.70e-18 86.7
Msa0280270 AT2G24570.1 53.968 63 29 0 306 368 240 302 2.09e-18 86.7
Msa0280270 AT1G29280.1 45.055 91 41 1 288 369 44 134 2.87e-18 85.1
Msa0280270 AT3G04670.1 48.235 85 41 1 287 368 237 321 2.93e-18 86.3
Msa0280270 AT4G23550.1 45.455 77 42 0 293 369 124 200 8.40e-18 84.7
Msa0280270 AT5G45050.4 45.882 85 45 1 286 369 1017 1101 8.47e-18 87.4
Msa0280270 AT5G45050.1 45.882 85 45 1 286 369 1156 1240 9.66e-18 87.0
Msa0280270 AT5G45050.2 45.882 85 45 1 286 369 1128 1212 1.01e-17 87.0
Msa0280270 AT4G24240.1 45.783 83 42 1 287 366 256 338 1.06e-17 84.7
Msa0280270 AT2G34830.1 43.158 95 44 2 284 369 182 275 1.26e-17 85.5
Msa0280270 AT2G34830.2 43.158 95 44 2 284 369 191 284 1.38e-17 85.5
Msa0280270 AT3G58710.2 55.738 61 27 0 309 369 70 130 1.93e-17 82.8
Msa0280270 AT3G58710.1 55.738 61 27 0 309 369 71 131 1.98e-17 82.8
Msa0280270 AT1G30650.1 43.158 95 44 2 284 369 184 277 2.36e-17 84.7
Msa0280270 AT2G30590.1 55.932 59 26 0 310 368 314 372 2.10e-16 81.3
Msa0280270 AT4G30935.1 40.000 105 58 3 266 369 290 390 2.51e-16 81.6
Msa0280270 AT5G07100.1 34.559 136 81 5 237 369 163 293 1.22e-15 78.2
Msa0280270 AT5G07100.1 43.056 72 40 1 302 373 110 180 1.40e-11 65.9
Msa0280270 AT5G07100.2 34.559 136 81 5 237 369 70 200 2.64e-15 75.5
Msa0280270 AT5G07100.2 42.466 73 41 1 302 374 17 88 3.40e-11 63.2
Msa0280270 AT5G07100.4 34.559 136 81 5 237 369 70 200 2.64e-15 75.5
Msa0280270 AT5G07100.4 42.466 73 41 1 302 374 17 88 3.40e-11 63.2
Msa0280270 AT5G07100.5 34.559 136 81 5 237 369 70 200 2.64e-15 75.5
Msa0280270 AT5G07100.5 42.466 73 41 1 302 374 17 88 3.40e-11 63.2
Msa0280270 AT5G07100.3 34.559 136 81 5 237 369 70 200 2.64e-15 75.5
Msa0280270 AT5G07100.3 42.466 73 41 1 302 374 17 88 3.40e-11 63.2
Msa0280270 AT2G04880.1 37.168 113 56 3 271 369 255 366 5.10e-15 77.8
Msa0280270 AT3G01080.2 48.485 66 33 1 304 369 339 403 5.26e-15 77.4
Msa0280270 AT3G01080.2 45.714 70 36 2 305 374 201 268 1.05e-13 73.6
Msa0280270 AT2G04880.2 37.168 113 56 3 271 369 231 342 5.40e-15 77.4
Msa0280270 AT3G01080.1 48.485 66 33 1 304 369 301 365 6.98e-15 77.0
Msa0280270 AT3G01080.1 45.714 70 36 2 305 374 163 230 1.47e-13 72.8
Msa0280270 AT4G26640.5 41.667 72 41 1 298 369 194 264 3.91e-14 74.3
Msa0280270 AT4G26640.5 48.438 64 31 2 307 370 33 94 5.50e-11 64.7
Msa0280270 AT4G26640.6 41.667 72 41 1 298 369 194 264 3.91e-14 74.3
Msa0280270 AT4G26640.6 48.438 64 31 2 307 370 33 94 5.50e-11 64.7
Msa0280270 AT4G26640.4 41.667 72 41 1 298 369 194 264 3.91e-14 74.3
Msa0280270 AT4G26640.4 48.438 64 31 2 307 370 33 94 5.50e-11 64.7
Msa0280270 AT4G26640.3 41.667 72 41 1 298 369 194 264 3.91e-14 74.3
Msa0280270 AT4G26640.3 48.438 64 31 2 307 370 33 94 5.50e-11 64.7
Msa0280270 AT4G26640.7 41.667 72 41 1 298 369 330 400 4.22e-14 75.1
Msa0280270 AT4G26640.7 37.383 107 57 3 264 370 134 230 2.73e-11 66.2
Msa0280270 AT3G01080.3 45.714 70 36 2 305 374 201 268 4.46e-14 73.6
Msa0280270 AT4G26640.2 41.667 72 41 1 298 369 370 440 5.00e-14 74.7
Msa0280270 AT4G26640.2 37.383 107 57 3 264 370 174 270 2.99e-11 66.2
Msa0280270 AT4G26640.1 41.667 72 41 1 298 369 298 368 5.46e-14 74.3
Msa0280270 AT4G26640.1 37.383 107 57 3 264 370 102 198 3.95e-11 65.5
Msa0280270 AT2G37260.1 41.463 82 43 2 289 366 325 405 7.26e-14 73.9
Msa0280270 AT2G37260.1 50.769 65 30 2 305 369 161 223 4.48e-12 68.2
Msa0280270 AT2G38470.1 51.471 68 31 2 303 370 178 243 7.54e-14 74.3
Msa0280270 AT2G38470.1 46.667 60 31 1 310 369 363 421 3.81e-13 72.0
Msa0280270 AT2G37260.3 41.463 82 43 2 289 366 245 325 1.21e-13 72.4
Msa0280270 AT2G37260.3 50.769 65 30 2 305 369 81 143 7.36e-12 67.0
Msa0280270 AT2G37260.2 41.463 82 43 2 289 366 243 323 1.23e-13 72.4
Msa0280270 AT2G37260.2 50.769 65 30 2 305 369 79 141 7.46e-12 67.0
Msa0280270 AT1G13960.1 43.939 66 36 1 304 369 404 468 1.24e-13 73.6
Msa0280270 AT1G13960.1 44.928 69 36 2 305 373 225 291 2.98e-12 69.3
Msa0280270 AT2G03340.1 43.939 66 36 1 304 369 410 474 1.54e-13 73.2
Msa0280270 AT2G03340.1 45.714 70 36 2 305 374 246 313 5.81e-12 68.2
Msa0280270 AT5G26170.1 49.206 63 31 1 304 366 108 169 1.75e-13 68.9
Msa0280270 AT1G13960.2 43.939 66 36 1 304 369 251 315 2.41e-13 71.6
Msa0280270 AT1G13960.2 44.928 69 36 2 305 373 72 138 6.66e-12 67.4
Msa0280270 AT4G22070.4 40.909 66 39 0 304 369 292 357 4.35e-13 72.0
Msa0280270 AT4G22070.3 40.909 66 39 0 304 369 292 357 4.35e-13 72.0
Msa0280270 AT4G22070.2 40.909 66 39 0 304 369 292 357 4.35e-13 72.0
Msa0280270 AT4G22070.1 40.909 66 39 0 304 369 292 357 4.35e-13 72.0
Msa0280270 AT1G80840.1 39.024 82 46 1 295 372 128 209 6.03e-13 70.1
Msa0280270 AT2G47260.1 37.363 91 55 2 279 369 145 233 9.99e-13 69.7
Msa0280270 AT4G04450.2 36.986 73 46 0 304 376 193 265 1.02e-12 70.5
Msa0280270 AT1G69810.2 42.667 75 42 1 304 377 140 214 1.30e-12 69.3
Msa0280270 AT1G62300.1 38.462 78 47 1 292 369 296 372 1.46e-12 70.1
Msa0280270 AT1G69810.1 46.377 69 36 1 304 371 198 266 1.73e-12 69.3
Msa0280270 AT2G30250.1 45.312 64 34 1 310 373 329 391 1.93e-12 69.3
Msa0280270 AT4G04450.1 36.986 73 46 0 304 376 287 359 1.95e-12 69.7
Msa0280270 AT1G69310.4 34.000 100 64 2 268 367 107 204 4.70e-12 67.0
Msa0280270 AT1G69310.2 34.000 100 64 2 268 367 107 204 4.70e-12 67.0
Msa0280270 AT1G69310.1 34.000 100 64 2 268 367 107 204 4.70e-12 67.0
Msa0280270 AT4G31800.2 41.667 60 35 0 310 369 112 171 1.02e-11 65.5
Msa0280270 AT1G29860.1 39.506 81 46 2 299 377 124 203 1.20e-11 65.9
Msa0280270 AT4G18170.1 36.047 86 53 2 289 374 153 236 1.22e-11 66.2
Msa0280270 AT5G56270.1 50.000 60 28 2 310 369 274 331 1.32e-11 67.4
Msa0280270 AT5G56270.1 43.333 60 33 1 310 369 488 546 1.80e-11 67.0
Msa0280270 AT4G31800.1 41.667 60 35 0 310 369 177 236 1.43e-11 65.9
Msa0280270 AT1G55600.1 38.356 73 43 2 298 369 295 366 1.62e-11 66.6
Msa0280270 AT3G62340.1 41.176 85 46 3 286 369 96 177 1.87e-11 65.1
Msa0280270 AT5G49520.1 45.000 60 32 1 310 369 222 280 2.24e-11 65.9
Msa0280270 AT1G18860.1 45.000 60 33 0 310 369 240 299 2.57e-11 66.2
Msa0280270 AT5G15130.2 38.961 77 45 1 295 369 169 245 2.59e-11 66.2
Msa0280270 AT4G26440.1 45.205 73 34 3 297 369 170 236 3.26e-11 65.9
Msa0280270 AT5G15130.1 38.961 77 45 1 295 369 211 287 3.49e-11 65.9
Msa0280270 AT5G43290.1 50.000 60 29 1 307 366 112 170 4.78e-11 63.9

Find 100 sgRNAs with CRISPR-Local

Find 197 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTCTGAAAGAGAATTTAT+TGG 0.190862 2_3:-14764327 Msa0280270:CDS
TGTTCTGAAAGAGAATTTAT+TGG 0.190862 2_3:-14764625 Msa0280270:CDS
ACAAGAGATGTTTGATTCAT+TGG 0.241614 2_3:+14764347 None:intergenic
TAGAAACCCAAACTAGTTCT+AGG 0.250090 2_3:-14766208 Msa0280270:CDS
ACAAAGTTCTTAATTTCTTC+TGG 0.267372 2_3:+14764739 None:intergenic
GTAAAACCCTAGAACTAGTT+TGG 0.270284 2_3:+14766201 None:intergenic
AAGGTGGTGGTAGTGGTAAT+TGG 0.290628 2_3:+14766518 None:intergenic
TATTATTAGTGATGTGGGTC+GGG 0.301991 2_3:+14766726 None:intergenic
CTAGTTTGGGTTTCTAGAAT+TGG 0.312695 2_3:+14766215 None:intergenic
CAAACCTCAGATCAAGAAAA+AGG 0.317679 2_3:-14766157 Msa0280270:intron
AGGTGGTGGTAGTGGTAATT+GGG 0.338809 2_3:+14766519 None:intergenic
GGTAATTGGGGTAATGATAG+TGG 0.346701 2_3:+14766532 None:intergenic
GACTTTAACCACCTCAATAC+TGG 0.366263 2_3:-14764237 Msa0280270:CDS
GACTTTAACCACCTCAATAC+TGG 0.366263 2_3:-14764535 Msa0280270:intron
CTTCAATGGATAATGATGAT+TGG 0.367851 2_3:-14767072 None:intergenic
GGGTGGTGGTTGAAGGGGTG+GGG 0.368488 2_3:+14766750 None:intergenic
TTCAATGGATAATGATGATT+GGG 0.373937 2_3:-14767071 None:intergenic
GGGAATGTGAATAGCGGTGG+TGG 0.376751 2_3:+14766577 None:intergenic
GCTTATATTGTGACGTATAA+AGG 0.377857 2_3:-14764781 Msa0280270:CDS
GTGGTGGTGGTTAGGATTTG+AGG 0.379807 2_3:+14766971 None:intergenic
GGGGTGGTGGTTGAAGGGGT+GGG 0.382660 2_3:+14766749 None:intergenic
GATTGTTGCTGATTTGTGTT+TGG 0.383802 2_3:+14766179 None:intergenic
TCTAACTCTATGAAGTCATT+TGG 0.386725 2_3:+14766848 None:intergenic
TACTGATGGTGGTGGTGGTT+AGG 0.393621 2_3:+14766963 None:intergenic
TCTGACATAGCTGTTATGTT+TGG 0.394725 2_3:+14764273 None:intergenic
TCTGACATAGCTGTTATGTT+TGG 0.394725 2_3:+14764571 None:intergenic
TAAAACCCTAGAACTAGTTT+GGG 0.406708 2_3:+14766202 None:intergenic
ACTAACACTAGGATATTGAT+TGG 0.429065 2_3:+14766400 None:intergenic
TATGTCAGAAGATTTATTGA+TGG 0.435423 2_3:-14764259 Msa0280270:CDS
TATGTCAGAAGATTTATTGA+TGG 0.435423 2_3:-14764557 Msa0280270:CDS
ATTAGTGATGTGGGTCGGGG+GGG 0.446462 2_3:+14766730 None:intergenic
TGGAGTTAGAGTTTCAGAAT+TGG 0.449537 2_3:+14766994 None:intergenic
TGTGTATGTTGTGGACTAGA+TGG 0.450832 2_3:+14766254 None:intergenic
AAAGGTGGAATTTCGATTTG+AGG 0.455769 2_3:+14766685 None:intergenic
ATATTATTAGTGATGTGGGT+CGG 0.457432 2_3:+14766725 None:intergenic
ACAAGAGATGTTTGATTCAT+CGG 0.457654 2_3:+14764645 None:intergenic
GGAATGTGAATAGCGGTGGT+GGG 0.459869 2_3:+14766578 None:intergenic
ATTATTAGTGATGTGGGTCG+GGG 0.469875 2_3:+14766727 None:intergenic
GAGCATAACATCTTCTACTT+CGG 0.472162 2_3:+14766610 None:intergenic
GTTAATATTATTAGTGATGT+GGG 0.476825 2_3:+14766721 None:intergenic
GCCAGAGACTAGCAATTCTC+AGG 0.477878 2_3:-14764376 Msa0280270:intron
TGAAGTCATTTGGTTTAAGC+GGG 0.482952 2_3:+14766858 None:intergenic
AGCGGTTGTGATTTCAATGC+AGG 0.486927 2_3:+14766301 None:intergenic
ATGAAGTCATTTGGTTTAAG+CGG 0.492100 2_3:+14766857 None:intergenic
ATAACACACCAAGGACATCT+TGG 0.504754 2_3:+14765992 None:intergenic
TTGCTTCTTTGTGTATGTTG+TGG 0.506190 2_3:+14766245 None:intergenic
TATTAGTGATGTGGGTCGGG+GGG 0.506513 2_3:+14766729 None:intergenic
AGAAACCCAAACTAGTTCTA+GGG 0.507577 2_3:-14766207 Msa0280270:CDS
TGTCCTTGGTGTGTTATATG+AGG 0.509380 2_3:-14765986 Msa0280270:CDS
GTTAGCGAAGGTGGTGGTAG+TGG 0.510257 2_3:+14766511 None:intergenic
GAATGTGAATAGCGGTGGTG+GGG 0.524266 2_3:+14766579 None:intergenic
GGTAGGAGTAGTGGCAGTCA+AGG 0.527115 2_3:+14766469 None:intergenic
CAAGGTGCTAATAGTGTTGG+CGG 0.530004 2_3:+14766487 None:intergenic
AGTTAGAGTTTCAGAATTGG+TGG 0.535695 2_3:+14766997 None:intergenic
GTTGGCGGGGTTAGCGAAGG+TGG 0.541707 2_3:+14766502 None:intergenic
TGTTAATATTATTAGTGATG+TGG 0.542659 2_3:+14766720 None:intergenic
GGTGGTGAGAGTAGTGATGG+TGG 0.545701 2_3:+14766556 None:intergenic
TTATTAGTGATGTGGGTCGG+GGG 0.546146 2_3:+14766728 None:intergenic
GATAGTGTTGGCAGTAGAGA+TGG 0.547801 2_3:+14766448 None:intergenic
GGAATAAAGCACCAGTATTG+AGG 0.547846 2_3:+14764226 None:intergenic
GGAATAAAGCACCAGTATTG+AGG 0.547846 2_3:+14764524 None:intergenic
GTAATTGGGGTAATGATAGT+GGG 0.548235 2_3:+14766533 None:intergenic
GATGTTACTGATGGTGGTGG+TGG 0.548240 2_3:+14766958 None:intergenic
GGCGGGGTTAGCGAAGGTGG+TGG 0.550477 2_3:+14766505 None:intergenic
AAGGTGCTAATAGTGTTGGC+GGG 0.550983 2_3:+14766488 None:intergenic
GTGGTGGTTAGGATTTGAGG+TGG 0.552967 2_3:+14766974 None:intergenic
AAAGAATGCCAAGATGTCCT+TGG 0.553216 2_3:-14766000 Msa0280270:CDS
AGTAGAGATGGTAGGAGTAG+TGG 0.558152 2_3:+14766460 None:intergenic
GGATATGATGTTACTGATGG+TGG 0.569473 2_3:+14766952 None:intergenic
TGAACTTTCAACAGACTCAT+GGG 0.572196 2_3:-14765958 Msa0280270:CDS
GGTGGCAGAAGTAGTAAAGG+TGG 0.572647 2_3:+14767015 None:intergenic
TTAAAGGGTCTCCACATCCA+AGG 0.575832 2_3:-14765908 Msa0280270:intron
ATTGGTGGCAGAAGTAGTAA+AGG 0.577716 2_3:+14767012 None:intergenic
GTGGTGAGAGTAGTGATGGT+GGG 0.578827 2_3:+14766557 None:intergenic
ATGTATGAAAACCTTGGATG+TGG 0.583670 2_3:+14765897 None:intergenic
AGTCAAGGTGCTAATAGTGT+TGG 0.585343 2_3:+14766484 None:intergenic
ATGAACTTTCAACAGACTCA+TGG 0.586902 2_3:-14765959 Msa0280270:CDS
TGTTGTTGTTCAATGAGTGT+TGG 0.587742 2_3:+14766653 None:intergenic
GTGTTGGCAGTAGAGATGGT+AGG 0.592781 2_3:+14766452 None:intergenic
GGTGGGAATGTGAATAGCGG+TGG 0.610840 2_3:+14766574 None:intergenic
TCATGGGCATGGAGTAAACA+TGG 0.611520 2_3:-14765942 Msa0280270:CDS
AGTGTTCGTCATGATAGTGT+TGG 0.615034 2_3:+14766436 None:intergenic
AATTGGGGTAATGATAGTGG+GGG 0.618091 2_3:+14766535 None:intergenic
TAATTGGGGTAATGATAGTG+GGG 0.618793 2_3:+14766534 None:intergenic
TTTCAACAGACTCATGGGCA+TGG 0.620196 2_3:-14765953 Msa0280270:CDS
ATCATCATAGCATGATGATG+TGG 0.621279 2_3:+14766919 None:intergenic
TATGTTGTGGACTAGATGGA+TGG 0.621555 2_3:+14766258 None:intergenic
TGATGATGTGGTACTTTGAG+AGG 0.630706 2_3:+14766931 None:intergenic
GGTGGTGGTAGTGGTAATTG+GGG 0.635605 2_3:+14766520 None:intergenic
TATGATGTTACTGATGGTGG+TGG 0.635627 2_3:+14766955 None:intergenic
AGTGTTGGCGGGGTTAGCGA+AGG 0.641015 2_3:+14766499 None:intergenic
AGAGGATATGATGTTACTGA+TGG 0.644683 2_3:+14766949 None:intergenic
TTCCCTCATATAACACACCA+AGG 0.662868 2_3:+14765983 None:intergenic
ATAAAGCACCAGTATTGAGG+TGG 0.663775 2_3:+14764229 None:intergenic
ATAAAGCACCAGTATTGAGG+TGG 0.663775 2_3:+14764527 None:intergenic
GAAGTCATTTGGTTTAAGCG+GGG 0.666201 2_3:+14766859 None:intergenic
AGGTGCTAATAGTGTTGGCG+GGG 0.672241 2_3:+14766489 None:intergenic
GTCCTTGGTGTGTTATATGA+GGG 0.673519 2_3:-14765985 Msa0280270:CDS
GATGGTGGGAATGTGAATAG+CGG 0.710352 2_3:+14766571 None:intergenic
GTATGCACGATCAAAATGAG+CGG 0.723045 2_3:+14766283 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAATAAAAAATATATAT+AGG - chr2_3:14765833-14765852 Msa0280270:intron 0.0%
!!! TTTATTTTATTTTTTGTCTT+TGG + chr2_3:14765823-14765842 None:intergenic 10.0%
!!! ATAGAAAAAGTAATTTTACA+GGG + chr2_3:14765583-14765602 None:intergenic 15.0%
!!! AATAGAAAAAGTAATTTTAC+AGG + chr2_3:14765584-14765603 None:intergenic 15.0%
!!! ATTTTAATCCTTATTTTGAT+GGG - chr2_3:14765692-14765711 Msa0280270:intron 15.0%
!! TTAATATGAATAATATTTGC+AGG - chr2_3:14765751-14765770 Msa0280270:intron 15.0%
!! TAATATGAATAATATTTGCA+GGG - chr2_3:14765752-14765771 Msa0280270:intron 15.0%
!! GTTAATATTATTAGTGATGT+GGG + chr2_3:14764570-14764589 None:intergenic 20.0%
!! TGTTAATATTATTAGTGATG+TGG + chr2_3:14764571-14764590 None:intergenic 20.0%
!!! TTTTTTCTGTAAGAAAAGAA+AGG + chr2_3:14765271-14765290 None:intergenic 20.0%
!! ATGGACAAAAATTCATTAAA+GGG - chr2_3:14765365-14765384 Msa0280270:intron 20.0%
!!! CATTTTAATCCTTATTTTGA+TGG - chr2_3:14765691-14765710 Msa0280270:intron 20.0%
!! AAAGTGAAAGATATAAACTT+AGG + chr2_3:14765960-14765979 None:intergenic 20.0%
!! TGATAAACAAACAATTGATA+CGG - chr2_3:14765994-14766013 Msa0280270:CDS 20.0%
!!! TTTATATAAAGGTATGCAAT+CGG + chr2_3:14766109-14766128 None:intergenic 20.0%
!! AAGAGATTGCATTATTTTCT+TGG + chr2_3:14764406-14764425 None:intergenic 25.0%
!!! GATAATGATTTTTGGAATAC+TGG + chr2_3:14764519-14764538 None:intergenic 25.0%
! GTAAGAAAAGAAAGGTAAAA+TGG + chr2_3:14765263-14765282 None:intergenic 25.0%
! CATGGACAAAAATTCATTAA+AGG - chr2_3:14765364-14765383 Msa0280270:intron 25.0%
!! TTTTGAATGTATGAAAACCT+TGG + chr2_3:14765400-14765419 None:intergenic 25.0%
!! AAATAGTGACAGTAAACTTT+TGG - chr2_3:14765505-14765524 Msa0280270:intron 25.0%
!!! AATTTTAGCCCATCAAAATA+AGG + chr2_3:14765703-14765722 None:intergenic 25.0%
! TATCAGTGCTCTTTATATAA+AGG + chr2_3:14766120-14766139 None:intergenic 25.0%
! TTAATCATTCTTTGCAATCT+TGG + chr2_3:14766218-14766237 None:intergenic 25.0%
!! TGATTTGTATCTTTAACAGT+AGG - chr2_3:14766245-14766264 Msa0280270:CDS 25.0%
! TCTTTAACAGTAGGAAAATA+CGG - chr2_3:14766254-14766273 Msa0280270:CDS 25.0%
!! GAAATCTATGAATGATTTGT+AGG - chr2_3:14766409-14766428 Msa0280270:CDS 25.0%
!!! TTCTCTACATATTTTTTTGC+TGG + chr2_3:14766468-14766487 None:intergenic 25.0%
!!! TCTTAATTTCTTCTGGTTTT+TGG + chr2_3:14766545-14766564 None:intergenic 25.0%
! ACAAAGTTCTTAATTTCTTC+TGG + chr2_3:14766552-14766571 None:intergenic 25.0%
!! GAAATTAAGAACTTTGTTGT+TGG - chr2_3:14766555-14766574 Msa0280270:CDS 25.0%
!!! TGTTCTGAAAGAGAATTTAT+TGG - chr2_3:14766663-14766682 Msa0280270:CDS 25.0%
! TATGTCAGAAGATTTATTGA+TGG - chr2_3:14766731-14766750 Msa0280270:CDS 25.0%
!! GAAATTAAGAACTTTGTTGT+TGG - chr2_3:14766555-14766574 Msa0280270:CDS 25.0%
!!! TGTTCTGAAAGAGAATTTAT+TGG - chr2_3:14766663-14766682 Msa0280270:CDS 25.0%
! TATGTCAGAAGATTTATTGA+TGG - chr2_3:14766731-14766750 Msa0280270:CDS 25.0%
! ATGAAGTCATTTGGTTTAAG+CGG + chr2_3:14764434-14764453 None:intergenic 30.0%
TCTAACTCTATGAAGTCATT+TGG + chr2_3:14764443-14764462 None:intergenic 30.0%
ATATTATTAGTGATGTGGGT+CGG + chr2_3:14764566-14764585 None:intergenic 30.0%
ACTAACACTAGGATATTGAT+TGG + chr2_3:14764891-14764910 None:intergenic 30.0%
GTTGAAAAATCACTAACACT+AGG + chr2_3:14764902-14764921 None:intergenic 30.0%
! TAAAACCCTAGAACTAGTTT+GGG + chr2_3:14765089-14765108 None:intergenic 30.0%
! ATTACCTTTTTCTTGATCTG+AGG + chr2_3:14765138-14765157 None:intergenic 30.0%
! GTTCGATGAGATTTAAGTTA+TGG + chr2_3:14765235-14765254 None:intergenic 30.0%
! CTATTTAAAACATGTGGTGT+GGG + chr2_3:14765491-14765510 None:intergenic 30.0%
! ACTATTTAAAACATGTGGTG+TGG + chr2_3:14765492-14765511 None:intergenic 30.0%
! CTGTCACTATTTAAAACATG+TGG + chr2_3:14765497-14765516 None:intergenic 30.0%
ATCCTTGCAATTTCAAAGAA+TGG - chr2_3:14765528-14765547 Msa0280270:intron 30.0%
!! TACCATTCTTTGAAATTGCA+AGG + chr2_3:14765533-14765552 None:intergenic 30.0%
AAGAACTATAACAAACACCA+GGG + chr2_3:14765638-14765657 None:intergenic 30.0%
TAAGAACTATAACAAACACC+AGG + chr2_3:14765639-14765658 None:intergenic 30.0%
TAAAAACCAAAAGCCAAAGA+TGG - chr2_3:14765790-14765809 Msa0280270:intron 30.0%
!!! AATAAACCATCTTTGGCTTT+TGG + chr2_3:14765799-14765818 None:intergenic 30.0%
! CTTTGGAAATAAACCATCTT+TGG + chr2_3:14765806-14765825 None:intergenic 30.0%
ACACATATATATGCCATGAT+GGG - chr2_3:14765863-14765882 Msa0280270:intron 30.0%
!! ATATGTTTTTGATCCCATCA+TGG + chr2_3:14765879-14765898 None:intergenic 30.0%
TTGCTCAATGGAAAAAGATA+CGG + chr2_3:14766177-14766196 None:intergenic 30.0%
! CTTTTTCCATTGAGCAATTA+AGG - chr2_3:14766180-14766199 Msa0280270:CDS 30.0%
ATGATATTCTAACAACCTAC+AGG + chr2_3:14766289-14766308 None:intergenic 30.0%
GCTTATATTGTGACGTATAA+AGG - chr2_3:14766507-14766526 Msa0280270:CDS 30.0%
ACAAGAGATGTTTGATTCAT+CGG + chr2_3:14766646-14766665 None:intergenic 30.0%
TCTAACCCAAATAGATTTCT+CGG - chr2_3:14766797-14766816 Msa0280270:intron 30.0%
TAACTCCGAGAAATCTATTT+GGG + chr2_3:14766805-14766824 None:intergenic 30.0%
ACAAGAGATGTTTGATTCAT+TGG + chr2_3:14766646-14766665 None:intergenic 30.0%
AGTTAGAGTTTCAGAATTGG+TGG + chr2_3:14764294-14764313 None:intergenic 35.0%
TGGAGTTAGAGTTTCAGAAT+TGG + chr2_3:14764297-14764316 None:intergenic 35.0%
AGAGGATATGATGTTACTGA+TGG + chr2_3:14764342-14764361 None:intergenic 35.0%
! ATCATCATAGCATGATGATG+TGG + chr2_3:14764372-14764391 None:intergenic 35.0%
! TGAAGTCATTTGGTTTAAGC+GGG + chr2_3:14764433-14764452 None:intergenic 35.0%
AATTGTGTTGGGATTGGAAT+TGG + chr2_3:14764477-14764496 None:intergenic 35.0%
TATTATTAGTGATGTGGGTC+GGG + chr2_3:14764565-14764584 None:intergenic 35.0%
! AAAGGTGGAATTTCGATTTG+AGG + chr2_3:14764606-14764625 None:intergenic 35.0%
TGTTGGAAGATGAAAAAAGG+TGG + chr2_3:14764621-14764640 None:intergenic 35.0%
!! GAGTGTTGGAAGATGAAAAA+AGG + chr2_3:14764624-14764643 None:intergenic 35.0%
! TGTTGTTGTTCAATGAGTGT+TGG + chr2_3:14764638-14764657 None:intergenic 35.0%
GAGCATAACATCTTCTACTT+CGG + chr2_3:14764681-14764700 None:intergenic 35.0%
! TAATTGGGGTAATGATAGTG+GGG + chr2_3:14764757-14764776 None:intergenic 35.0%
GTAATTGGGGTAATGATAGT+GGG + chr2_3:14764758-14764777 None:intergenic 35.0%
TTGCTTCTTTGTGTATGTTG+TGG + chr2_3:14765046-14765065 None:intergenic 35.0%
!! CTAGTTTGGGTTTCTAGAAT+TGG + chr2_3:14765076-14765095 None:intergenic 35.0%
! TAGAAACCCAAACTAGTTCT+AGG - chr2_3:14765080-14765099 Msa0280270:intron 35.0%
!! AGAAACCCAAACTAGTTCTA+GGG - chr2_3:14765081-14765100 Msa0280270:intron 35.0%
GTAAAACCCTAGAACTAGTT+TGG + chr2_3:14765090-14765109 None:intergenic 35.0%
!! GATTGTTGCTGATTTGTGTT+TGG + chr2_3:14765112-14765131 None:intergenic 35.0%
CAAACCTCAGATCAAGAAAA+AGG - chr2_3:14765131-14765150 Msa0280270:intron 35.0%
ATGAACTTTCAACAGACTCA+TGG - chr2_3:14765329-14765348 Msa0280270:intron 35.0%
TGAACTTTCAACAGACTCAT+GGG - chr2_3:14765330-14765349 Msa0280270:intron 35.0%
ATGTATGAAAACCTTGGATG+TGG + chr2_3:14765394-14765413 None:intergenic 35.0%
CATTGCAAATATTCCACCTT+TGG - chr2_3:14765604-14765623 Msa0280270:intron 35.0%
CACACATATATATGCCATGA+TGG - chr2_3:14765862-14765881 Msa0280270:intron 35.0%
AAGAAATATGCCACTCATTG+TGG - chr2_3:14766076-14766095 Msa0280270:intron 35.0%
AGAAATATGCCACTCATTGT+GGG - chr2_3:14766077-14766096 Msa0280270:intron 35.0%
!!! TGTGGAAAGTTGTTGGTTTT+TGG + chr2_3:14766152-14766171 None:intergenic 35.0%
TTCTTTGCAATCTTGGAAAG+CGG + chr2_3:14766211-14766230 None:intergenic 35.0%
!! CAGCATCATTTTGTTATGTG+AGG + chr2_3:14766583-14766602 None:intergenic 35.0%
TCTGACATAGCTGTTATGTT+TGG + chr2_3:14766720-14766739 None:intergenic 35.0%
CTAACTCCGAGAAATCTATT+TGG + chr2_3:14766806-14766825 None:intergenic 35.0%
!! CAGCATCATTTTGTTATGTG+AGG + chr2_3:14766583-14766602 None:intergenic 35.0%
TCTGACATAGCTGTTATGTT+TGG + chr2_3:14766720-14766739 None:intergenic 35.0%
! ATTGGTGGCAGAAGTAGTAA+AGG + chr2_3:14764279-14764298 None:intergenic 40.0%
!! TATGATGTTACTGATGGTGG+TGG + chr2_3:14764336-14764355 None:intergenic 40.0%
GGATATGATGTTACTGATGG+TGG + chr2_3:14764339-14764358 None:intergenic 40.0%
! TGATGATGTGGTACTTTGAG+AGG + chr2_3:14764360-14764379 None:intergenic 40.0%
! GAAGTCATTTGGTTTAAGCG+GGG + chr2_3:14764432-14764451 None:intergenic 40.0%
AGGGATAATTGTGTTGGGAT+TGG + chr2_3:14764483-14764502 None:intergenic 40.0%
GGGTTAGGGATAATTGTGTT+GGG + chr2_3:14764488-14764507 None:intergenic 40.0%
!!! TTTTGGAATACTGGTGCTAG+CGG + chr2_3:14764510-14764529 None:intergenic 40.0%
ATTATTAGTGATGTGGGTCG+GGG + chr2_3:14764564-14764583 None:intergenic 40.0%
!! AATTGGGGTAATGATAGTGG+GGG + chr2_3:14764756-14764775 None:intergenic 40.0%
GGTAATTGGGGTAATGATAG+TGG + chr2_3:14764759-14764778 None:intergenic 40.0%
! AGTCAAGGTGCTAATAGTGT+TGG + chr2_3:14764807-14764826 None:intergenic 40.0%
! AGTGTTCGTCATGATAGTGT+TGG + chr2_3:14764855-14764874 None:intergenic 40.0%
GTATGCACGATCAAAATGAG+CGG + chr2_3:14765008-14765027 None:intergenic 40.0%
!! TATGTTGTGGACTAGATGGA+TGG + chr2_3:14765033-14765052 None:intergenic 40.0%
!! TGTGTATGTTGTGGACTAGA+TGG + chr2_3:14765037-14765056 None:intergenic 40.0%
AAAGAATGCCAAGATGTCCT+TGG - chr2_3:14765288-14765307 Msa0280270:intron 40.0%
ATAACACACCAAGGACATCT+TGG + chr2_3:14765299-14765318 None:intergenic 40.0%
! TGTCCTTGGTGTGTTATATG+AGG - chr2_3:14765302-14765321 Msa0280270:intron 40.0%
! GTCCTTGGTGTGTTATATGA+GGG - chr2_3:14765303-14765322 Msa0280270:intron 40.0%
TTCCCTCATATAACACACCA+AGG + chr2_3:14765308-14765327 None:intergenic 40.0%
ATGGAAAAAGATACGGTGTG+TGG + chr2_3:14766170-14766189 None:intergenic 40.0%
TTCCATTGAGCAATTAAGGC+TGG - chr2_3:14766184-14766203 Msa0280270:CDS 40.0%
TCTTTGCAATCTTGGAAAGC+GGG + chr2_3:14766210-14766229 None:intergenic 40.0%
AACAGTAGGAAAATACGGTG+TGG - chr2_3:14766259-14766278 Msa0280270:CDS 40.0%
GACTTTAACCACCTCAATAC+TGG - chr2_3:14766753-14766772 Msa0280270:CDS 40.0%
! ATAAAGCACCAGTATTGAGG+TGG + chr2_3:14766764-14766783 None:intergenic 40.0%
! GGAATAAAGCACCAGTATTG+AGG + chr2_3:14766767-14766786 None:intergenic 40.0%
! TGGTGCTTTATTCCCATAGT+TGG - chr2_3:14766773-14766792 Msa0280270:intron 40.0%
! GGTGCTTTATTCCCATAGTT+GGG - chr2_3:14766774-14766793 Msa0280270:intron 40.0%
! TTTGGGTTAGACCCAACTAT+GGG + chr2_3:14766788-14766807 None:intergenic 40.0%
! ATTTGGGTTAGACCCAACTA+TGG + chr2_3:14766789-14766808 None:intergenic 40.0%
GACTTTAACCACCTCAATAC+TGG - chr2_3:14766753-14766772 Msa0280270:CDS 40.0%
! ATAAAGCACCAGTATTGAGG+TGG + chr2_3:14766764-14766783 None:intergenic 40.0%
! GGAATAAAGCACCAGTATTG+AGG + chr2_3:14766767-14766786 None:intergenic 40.0%
GGGGTTAGGGATAATTGTGT+TGG + chr2_3:14764489-14764508 None:intergenic 45.0%
! TTTGGAATACTGGTGCTAGC+GGG + chr2_3:14764509-14764528 None:intergenic 45.0%
! GGGGTGGGGATAATGATTTT+TGG + chr2_3:14764527-14764546 None:intergenic 45.0%
TTATTAGTGATGTGGGTCGG+GGG + chr2_3:14764563-14764582 None:intergenic 45.0%
!! GATGGTGGGAATGTGAATAG+CGG + chr2_3:14764720-14764739 None:intergenic 45.0%
!! AGGTGGTGGTAGTGGTAATT+GGG + chr2_3:14764772-14764791 None:intergenic 45.0%
!! AAGGTGGTGGTAGTGGTAAT+TGG + chr2_3:14764773-14764792 None:intergenic 45.0%
!! AAGGTGCTAATAGTGTTGGC+GGG + chr2_3:14764803-14764822 None:intergenic 45.0%
! CAAGGTGCTAATAGTGTTGG+CGG + chr2_3:14764804-14764823 None:intergenic 45.0%
AGTAGAGATGGTAGGAGTAG+TGG + chr2_3:14764831-14764850 None:intergenic 45.0%
!! GATAGTGTTGGCAGTAGAGA+TGG + chr2_3:14764843-14764862 None:intergenic 45.0%
AGCGGTTGTGATTTCAATGC+AGG + chr2_3:14764990-14765009 None:intergenic 45.0%
TTTCAACAGACTCATGGGCA+TGG - chr2_3:14765335-14765354 Msa0280270:intron 45.0%
TCATGGGCATGGAGTAAACA+TGG - chr2_3:14765346-14765365 Msa0280270:intron 45.0%
TTAAAGGGTCTCCACATCCA+AGG - chr2_3:14765380-14765399 Msa0280270:intron 45.0%
CAGGGACAAAAAACCAAAGG+TGG + chr2_3:14765620-14765639 None:intergenic 45.0%
!!! CACCTTTGGTTTTTTGTCCC+TGG - chr2_3:14765618-14765637 Msa0280270:intron 45.0%
CACCAGGGACAAAAAACCAA+AGG + chr2_3:14765623-14765642 None:intergenic 45.0%
ATATGCCACTCATTGTGGGA+CGG - chr2_3:14766081-14766100 Msa0280270:intron 45.0%
TACGGTGTGTGGAAAGTTGT+TGG + chr2_3:14766159-14766178 None:intergenic 45.0%
! ACCTGAGAATTGCTAGTCTC+TGG + chr2_3:14766618-14766637 None:intergenic 45.0%
! ACCTGAGAATTGCTAGTCTC+TGG + chr2_3:14766618-14766637 None:intergenic 45.0%
GGTGGCAGAAGTAGTAAAGG+TGG + chr2_3:14764276-14764295 None:intergenic 50.0%
GTGGTGGTTAGGATTTGAGG+TGG + chr2_3:14764317-14764336 None:intergenic 50.0%
!! GTGGTGGTGGTTAGGATTTG+AGG + chr2_3:14764320-14764339 None:intergenic 50.0%
!! TACTGATGGTGGTGGTGGTT+AGG + chr2_3:14764328-14764347 None:intergenic 50.0%
!! GATGTTACTGATGGTGGTGG+TGG + chr2_3:14764333-14764352 None:intergenic 50.0%
! TTGGAATACTGGTGCTAGCG+GGG + chr2_3:14764508-14764527 None:intergenic 50.0%
TATTAGTGATGTGGGTCGGG+GGG + chr2_3:14764562-14764581 None:intergenic 50.0%
GAATGTGAATAGCGGTGGTG+GGG + chr2_3:14764712-14764731 None:intergenic 50.0%
GGAATGTGAATAGCGGTGGT+GGG + chr2_3:14764713-14764732 None:intergenic 50.0%
GTGGTGAGAGTAGTGATGGT+GGG + chr2_3:14764734-14764753 None:intergenic 50.0%
!! GGTGGTGGTAGTGGTAATTG+GGG + chr2_3:14764771-14764790 None:intergenic 50.0%
!! AGGTGCTAATAGTGTTGGCG+GGG + chr2_3:14764802-14764821 None:intergenic 50.0%
! GTGTTGGCAGTAGAGATGGT+AGG + chr2_3:14764839-14764858 None:intergenic 50.0%
GTGGTGTGGGTGTGTGTATT+AGG + chr2_3:14765478-14765497 None:intergenic 50.0%
GGCCAGCCTTAATTGCTCAA+TGG + chr2_3:14766189-14766208 None:intergenic 50.0%
ATACGGTGTGGACAACCTGT+AGG - chr2_3:14766271-14766290 Msa0280270:CDS 50.0%
GCCAGAGACTAGCAATTCTC+AGG - chr2_3:14766614-14766633 Msa0280270:CDS 50.0%
GCCAGAGACTAGCAATTCTC+AGG - chr2_3:14766614-14766633 Msa0280270:CDS 50.0%
! TACTGGTGCTAGCGGGGTTA+GGG + chr2_3:14764502-14764521 None:intergenic 55.0%
! ATACTGGTGCTAGCGGGGTT+AGG + chr2_3:14764503-14764522 None:intergenic 55.0%
ATTAGTGATGTGGGTCGGGG+GGG + chr2_3:14764561-14764580 None:intergenic 55.0%
GGGAATGTGAATAGCGGTGG+TGG + chr2_3:14764714-14764733 None:intergenic 55.0%
GGTGGGAATGTGAATAGCGG+TGG + chr2_3:14764717-14764736 None:intergenic 55.0%
GGTGGTGAGAGTAGTGATGG+TGG + chr2_3:14764735-14764754 None:intergenic 55.0%
!! TGGGGTAATGATAGTGGGGG+TGG + chr2_3:14764753-14764772 None:intergenic 55.0%
! GTTAGCGAAGGTGGTGGTAG+TGG + chr2_3:14764780-14764799 None:intergenic 55.0%
GGTAGGAGTAGTGGCAGTCA+AGG + chr2_3:14764822-14764841 None:intergenic 55.0%
GTAATGTGTGCGCGCGCAAA+TGG - chr2_3:14766342-14766361 Msa0280270:CDS 55.0%
GGGGGTGGTGAGAGTAGTGA+TGG + chr2_3:14764738-14764757 None:intergenic 60.0%
!! AGTGTTGGCGGGGTTAGCGA+AGG + chr2_3:14764792-14764811 None:intergenic 60.0%
CGGTTCCGTCCCACAATGAG+TGG + chr2_3:14766089-14766108 None:intergenic 60.0%
! GGGTGGTGGTTGAAGGGGTG+GGG + chr2_3:14764541-14764560 None:intergenic 65.0%
! GGGGTGGTGGTTGAAGGGGT+GGG + chr2_3:14764542-14764561 None:intergenic 65.0%
! TCGGGGGGGTGGTGGTTGAA+GGG + chr2_3:14764547-14764566 None:intergenic 65.0%
! GTTGGCGGGGTTAGCGAAGG+TGG + chr2_3:14764789-14764808 None:intergenic 65.0%
! GGGGGTGGTGGTTGAAGGGG+TGG + chr2_3:14764543-14764562 None:intergenic 70.0%
! CGGGGGGGTGGTGGTTGAAG+GGG + chr2_3:14764546-14764565 None:intergenic 70.0%
! GTCGGGGGGGTGGTGGTTGA+AGG + chr2_3:14764548-14764567 None:intergenic 70.0%
AGTGATGTGGGTCGGGGGGG+TGG + chr2_3:14764558-14764577 None:intergenic 70.0%
! GGCGGGGTTAGCGAAGGTGG+TGG + chr2_3:14764786-14764805 None:intergenic 70.0%
GATGTGGGTCGGGGGGGTGG+TGG + chr2_3:14764555-14764574 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 14764221 14767089 14764221 ID=Msa0280270;Name=Msa0280270
chr2_3 mRNA 14764221 14767089 14764221 ID=Msa0280270-mRNA-1;Parent=Msa0280270;Name=Msa0280270-mRNA-1;_AED=0.10;_eAED=0.10;_QI=0|0|0|1|0.4|0.16|6|0|479
chr2_3 exon 14766854 14767089 14766854 ID=Msa0280270-mRNA-1:exon:6252;Parent=Msa0280270-mRNA-1
chr2_3 exon 14766158 14766790 14766158 ID=Msa0280270-mRNA-1:exon:6251;Parent=Msa0280270-mRNA-1
chr2_3 exon 14765909 14766025 14765909 ID=Msa0280270-mRNA-1:exon:6250;Parent=Msa0280270-mRNA-1
chr2_3 exon 14764738 14764879 14764738 ID=Msa0280270-mRNA-1:exon:6249;Parent=Msa0280270-mRNA-1
chr2_3 exon 14764536 14764674 14764536 ID=Msa0280270-mRNA-1:exon:6248;Parent=Msa0280270-mRNA-1
chr2_3 exon 14764221 14764393 14764221 ID=Msa0280270-mRNA-1:exon:6247;Parent=Msa0280270-mRNA-1
chr2_3 CDS 14766854 14767089 14766854 ID=Msa0280270-mRNA-1:cds;Parent=Msa0280270-mRNA-1
chr2_3 CDS 14766158 14766790 14766158 ID=Msa0280270-mRNA-1:cds;Parent=Msa0280270-mRNA-1
chr2_3 CDS 14765909 14766025 14765909 ID=Msa0280270-mRNA-1:cds;Parent=Msa0280270-mRNA-1
chr2_3 CDS 14764738 14764879 14764738 ID=Msa0280270-mRNA-1:cds;Parent=Msa0280270-mRNA-1
chr2_3 CDS 14764536 14764674 14764536 ID=Msa0280270-mRNA-1:cds;Parent=Msa0280270-mRNA-1
chr2_3 CDS 14764221 14764393 14764221 ID=Msa0280270-mRNA-1:cds;Parent=Msa0280270-mRNA-1
Gene Sequence

>Msa0280270

ATGGATAATGATGATTGGGATCTCTTTTCTATTGTGCGTAGCTGCAACACTACCACCTTTACTACTTCTGCCACCAATTCTGAAACTCTAACTCCACCTCAAATCCTAACCACCACCACCATCAGTAACATCATATCCTCTCAAAGTACCACATCATCATGCTATGATGATTTTACTTTTATCCAAGAAAATAATGCAATCTCTTTTGCCCCGCTTAAACCAAATGACTTCATAGATATTCCAAAAATCATTATCCCCACCCCTTCAACCACCACCCCCCCGACCCACATCACTAATAATATTAACACTAGTGTTCATGCCTCAAATCGAAATTCCACCTTTTTTCATCTTCCAACACTCATTGAACAACAACAAATGCAACGAATTGAGTTCACCGAAGTAGAAGATGTTATGCTCAAATTCAACCCCACCACCGCTATTCACATTCCCACCATCACTACTCTCACCACCCCCACTATCATTACCCCAATTACCACTACCACCACCTTCGCTAACCCCGCCAACACTATTAGCACCTTGACTGCCACTACTCCTACCATCTCTACTGCCAACACTATCATGACGAACACTAGCGTTCATGAAACCAATCAATATCCTAGTGTTAGTGATTTTTCAACACTCGTTGAACAACAACATATTCATCAAAGCAACAACAATCAAGTGTTTGTTTCGAAACATGTTGCCTGCATTGAAATCACAACCGCTCATTTTGATCGTGCATACAGCCATCCATCTAGTCCACAACATACACAAAGAAGCAATCAACTTCCAATTCTAGAAACCCAAACTAGTTCTAGGGTTTTACCAAACACAAATCAGCAACAATCAAACCTCAGATCAAGAAAAAGAAAAAAGAATGCCAAGATGTCCTTGGTGTGTTATATGAGGGAAGATGAACTTTCAACAGACTCATGGGCATGGAGTAAACATGGACAAAAATTCATTAAAGGGTCTCCACATCCAAGGTGCTATTATAAGTGCAGCAGTTTCGCAAATTGTCCAGCAAAAAAATATGTAGAGAAAAGCAAAACTCATGAGAGCGCTTATATTGTGACGTATAAAGGAGAGCATGATCACCAAAAACCAGAAGAAATTAAGAACTTTGTTGTTTTTTCCGATGAATCAAACATCTCTTGTTCTGAAAGAGAATTTATTGGAAACTCTAATGATGATGATGACATTTTGATACCAAACATAACAGCTATGTCAGAAGATTTATTGATGGACTTTAACCACCTCAATACTGAGACTAGCAATTCTCAGGTTTTTTCCAATGAATCAAACATCTCTTGTTCTGAAAGAGAATTTATTGGAAACTCTAATGATGATGATGACATTTTGATACCAAACATAACAGCTATGTCAGAAGATTTATTGATGGACTTTAACCACCTCAATACTGGTGCTTTATTCCCATAG
Protein sequence

>Msa0280270

MDNDDWDLFSIVRSCNTTTFTTSATNSETLTPPQILTTTTISNIISSQSTTSSCYDDFTFIQENNAISFAPLKPNDFIDIPKIIIPTPSTTTPPTHITNNINTSVHASNRNSTFFHLPTLIEQQQMQRIEFTEVEDVMLKFNPTTAIHIPTITTLTTPTIITPITTTTTFANPANTISTLTATTPTISTANTIMTNTSVHETNQYPSVSDFSTLVEQQHIHQSNNNQVFVSKHVACIEITTAHFDRAYSHPSSPQHTQRSNQLPILETQTSSRVLPNTNQQQSNLRSRKRKKNAKMSLVCYMREDELSTDSWAWSKHGQKFIKGSPHPRCYYKCSSFANCPAKKYVEKSKTHESAYIVTYKGEHDHQKPEEIKNFVVFSDESNISCSEREFIGNSNDDDDILIPNITAMSEDLLMDFNHLNTETSNSQVFSNESNISCSEREFIGNSNDDDDILIPNITAMSEDLLMDFNHLNTGALFP*