AlfalfaGEDB | Msa0280530

M. sativa cultivar zhongmu-4 / Msa0280530

KEGG: K14494
GO: -
PFAM: PF03514.17

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0280530	XP_003594754.1	72.964	577	95	10	1	528	1	565	0.0	793
Msa0280530	XP_003594754.1	93.162	585	27	3	529	1112	15	587	0.0	1104

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0280530	sp\|Q8GXW1\|RGL2_ARATH	31.043	393	252	7	722	1111	170	546	5.50e-53	198
Msa0280530	sp\|Q8GXW1\|RGL2_ARATH	29.006	362	214	12	182	520	176	517	2.31e-32	137

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0280530	A2Q2X8	72.964	577	95	10	1	528	1	565	0.0	793
Msa0280530	A2Q2X8	93.162	585	27	3	529	1112	15	587	0.0	1104

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TFs/TRs:

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Gene ID	Type	Classification
Msa0280530	TF	GRAS

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0280530	MtrunA17_Chr2g0294461	87.986	591	46	7	529	1112	15	587	0.0	1028
Msa0280530	MtrunA17_Chr2g0294461	73.875	578	88	9	1	528	1	565	0.0	812
Msa0280530	MtrunA17_Chr2g0294471	80.560	571	94	8	550	1112	17	578	0.0	910
Msa0280530	MtrunA17_Chr2g0294471	67.642	581	109	12	1	528	2	556	0.0	719
Msa0280530	MtrunA17_Chr7g0270031	44.511	501	251	7	612	1112	83	556	1.02e-129	406
Msa0280530	MtrunA17_Chr7g0270031	39.379	419	195	10	153	528	132	534	2.89e-80	273
Msa0280530	MtrunA17_Chr1g0194621	42.230	547	272	11	572	1112	12	520	3.82e-126	396
Msa0280530	MtrunA17_Chr1g0194621	39.141	419	193	11	157	528	95	498	5.76e-74	254
Msa0280530	MtrunA17_Chr8g0376381	32.051	390	239	6	724	1111	158	523	5.79e-54	197
Msa0280530	MtrunA17_Chr8g0376381	30.255	314	175	7	229	520	203	494	9.63e-35	140

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0280530	AT3G03450.1	31.043	393	252	7	722	1111	170	546	5.59e-54	198
Msa0280530	AT3G03450.1	29.006	362	214	12	182	520	176	517	2.35e-33	137
Msa0280530	AT2G01570.1	29.847	392	253	6	722	1111	211	582	2.90e-52	194
Msa0280530	AT2G01570.1	29.781	319	180	9	226	520	255	553	4.63e-33	136
Msa0280530	AT5G17490.1	29.820	389	248	8	726	1111	151	517	4.09e-50	186
Msa0280530	AT5G17490.1	30.449	312	172	12	226	513	191	481	9.55e-32	131
Msa0280530	AT1G14920.1	29.592	392	254	6	722	1111	159	530	1.16e-49	185
Msa0280530	AT1G14920.1	29.299	314	182	9	229	520	206	501	7.44e-30	126
Msa0280530	AT1G66350.1	29.306	389	249	6	724	1111	144	507	4.07e-49	183
Msa0280530	AT1G66350.1	25.806	403	241	14	131	513	107	471	3.21e-29	124

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Find 146 sgRNAs with CRISPR-Local

Find 269 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATCATTAACTGGATGAA+GGG	0.532257	2_3:+15353263	None:intergenic
AAACTGAGAGTAGACCGCAA+TGG	0.750992	2_3:+15352435	None:intergenic
AAATCCGAAAAGGGTTACAA+TGG	0.612089	2_3:-15352690	Msa0280530:CDS
AAATTCGAAAAGGGTGTCAA+TGG	0.472001	2_3:-15349309	Msa0280530:CDS
AACCATAAATCCTATAGTAA+TGG	0.541375	2_3:-15348972	Msa0280530:CDS
AACCATTACTATAGGATTTA+TGG	0.158396	2_3:+15348970	None:intergenic
AACTATAAATCCTATAGTAA+TGG	0.549134	2_3:-15352353	Msa0280530:CDS
AAGAAGTTTGGTAGAATTGT+TGG	0.375164	2_3:+15353355	None:intergenic
AAGAGGTTACTACCTGATGA+AGG	0.555954	2_3:-15353291	Msa0280530:CDS
AAGATAACAGCTATTGAGAG+CGG	0.640580	2_3:-15349232	Msa0280530:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GTTGAGTTTCAATATAGTTG+AGG	-	chr2_3:15348582-15348601	Msa0280530:CDS	30.0%
GGTTTCCCAAAAAATATCAT+TGG	+	chr2_3:15348623-15348642	None:intergenic	30.0%
AAGAAGTTTGGTAGAATTGT+TGG	+	chr2_3:15348909-15348928	None:intergenic	30.0%
CAAAATCATTAACTGGATGA+AGG	+	chr2_3:15349002-15349021	None:intergenic	30.0%
CAGAGAAACAAAAACCAAAA+GGG	+	chr2_3:15349149-15349168	None:intergenic	30.0%
GAGTCTTTATCTTCAAAGAA+AGG	-	chr2_3:15349282-15349301	Msa0280530:CDS	30.0%
ACAAAGAATCGACAAAGAAA+CGG	-	chr2_3:15349350-15349369	Msa0280530:CDS	30.0%
GACAAACAATATGCAAAAGA+TGG	-	chr2_3:15349389-15349408	Msa0280530:CDS	30.0%
TCATGGAATTAGGAATATTG+TGG	-	chr2_3:15350085-15350104	Msa0280530:CDS	30.0%
TTTGTATCAAGCTGAATTGA+TGG	-	chr2_3:15350211-15350230	Msa0280530:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_3	gene	15348556	15353727	15348556	ID=Msa0280530;Name=Msa0280530
chr2_3	mRNA	15348556	15353727	15348556	ID=Msa0280530-mRNA-1;Parent=Msa0280530;Name=Msa0280530-mRNA-1;_AED=0.03;_eAED=0.05;_QI=0\|0\|0\|1\|0\|0\|9\|0\|1112
chr2_3	exon	15353550	15353727	15353550	ID=Msa0280530-mRNA-1:exon:6403;Parent=Msa0280530-mRNA-1
chr2_3	exon	15353347	15353537	15353347	ID=Msa0280530-mRNA-1:exon:6402;Parent=Msa0280530-mRNA-1
chr2_3	exon	15353089	15353331	15353089	ID=Msa0280530-mRNA-1:exon:6401;Parent=Msa0280530-mRNA-1
chr2_3	exon	15353000	15353063	15353000	ID=Msa0280530-mRNA-1:exon:6400;Parent=Msa0280530-mRNA-1
chr2_3	exon	15352750	15352979	15352750	ID=Msa0280530-mRNA-1:exon:6399;Parent=Msa0280530-mRNA-1
chr2_3	exon	15352502	15352731	15352502	ID=Msa0280530-mRNA-1:exon:6398;Parent=Msa0280530-mRNA-1
chr2_3	exon	15352278	15352489	15352278	ID=Msa0280530-mRNA-1:exon:6397;Parent=Msa0280530-mRNA-1
chr2_3	exon	15352005	15352241	15352005	ID=Msa0280530-mRNA-1:exon:6396;Parent=Msa0280530-mRNA-1
chr2_3	exon	15348556	15350309	15348556	ID=Msa0280530-mRNA-1:exon:6395;Parent=Msa0280530-mRNA-1
chr2_3	CDS	15353550	15353727	15353550	ID=Msa0280530-mRNA-1:cds;Parent=Msa0280530-mRNA-1
chr2_3	CDS	15353347	15353537	15353347	ID=Msa0280530-mRNA-1:cds;Parent=Msa0280530-mRNA-1
chr2_3	CDS	15353089	15353331	15353089	ID=Msa0280530-mRNA-1:cds;Parent=Msa0280530-mRNA-1
chr2_3	CDS	15353000	15353063	15353000	ID=Msa0280530-mRNA-1:cds;Parent=Msa0280530-mRNA-1
chr2_3	CDS	15352750	15352979	15352750	ID=Msa0280530-mRNA-1:cds;Parent=Msa0280530-mRNA-1
chr2_3	CDS	15352502	15352731	15352502	ID=Msa0280530-mRNA-1:cds;Parent=Msa0280530-mRNA-1
chr2_3	CDS	15352278	15352489	15352278	ID=Msa0280530-mRNA-1:cds;Parent=Msa0280530-mRNA-1
chr2_3	CDS	15352005	15352241	15352005	ID=Msa0280530-mRNA-1:cds;Parent=Msa0280530-mRNA-1
chr2_3	CDS	15348556	15350309	15348556	ID=Msa0280530-mRNA-1:cds;Parent=Msa0280530-mRNA-1

Gene Sequence

>Msa0280530

ATGGAAAACTTGTGCAATTTTGGTGAGTTGAGTTTCAATATAGTTGAGGATAAACTTAGTTCATCCAATGATATTTTTTGGGAAACCAAGAAACTTAGTGAAATGAAAAATGTTCAATTTTCTGTAGAAGAAGATCTAGGAGACTATAATGGAACTAAGTCTCTTTGCACTAATTTTGATGATCCATCACAAGAAGATGATTTCCTAAAGTATCATCACCAAGAACAAGGATCATATCAAGATTATGAAACTTTTGATAACCTACACTTTGACATGGTTCATTTTGATGAACAACAATGTCCAGCAAAAGACCCTCCACTTTGTGACACAAAAAAGGACCAACAATTCTACCAAACTTCTTTAGCACCGAACTATGGCAAATGCTTCAAGAGGTTACTACCTGATGAAGGAAAAACCCTTCATCCAGTTAATGATTTTGGTTTGATCACAGATAATGAAAATGAGAGTAAGTTGTCAACTGAAGATATCATGAAGATAGCTGGAACAAGGTTCATACAATCATCATCTTCGTCGCAATCTGCATCAGGTTTGATTCTTAATCACCCTTTTGGTTTTTGTTTCTCTGGTTTATCTGATGAAGAGAAAGAAGATCTTGTGCTGAGAAAGTCGGATATCAACAATATGAACGAGCTAAAAAATTGTTATCGCAAATTGAGTAACCCCGTTAAGCGCGTGGTTCATTATTTTGCCGAAGCTCTTCAACAAAGAATCGACAAAGAAACGGGAAGAGTTTCTGTTTCGACAAACAATATGCAAAAGATGGGACCTTTATTTGATCCTCAAGAGGTAACCAAGGATCTAAATCCAGCCCTTGTCGCGTTTTTTGAAGATCTTCCATTTTGTAAAATTTCAATGTTCACATGTGTACAAACTTTAATAGAAAATATTCATGTCATTGATCTCGAAATCCGAAAAGGGTTACAATGGACAATCCTAATGCAAGCACTTCAATTGAGAAACAAATGTCCCCTTGAGCTTCTAAAGATAACGGCTATTGTGACTGGAAACATAGACACTTCGAAGCATATAGTTGAAGACACTGGTAAAAGATTGAAGGACTTTGCGCGATATTTGAAAATACCTTTCTTATTCGATACAATTATTGTATCCGATTTAGAAGATCTTTTCAAGATAGATTCTGAGGAAACCATTGCGGTCTACTCTCAGTTTGCACTTCAAAGCAAGATTCAACAATCAGACCAACTTGAAACAATCATGAGAGTGGTTAGAACTATAAATCCTATAGTAATGGTTGTCGCGGAGATTGAAGCTAATCACAATTCTAAATCTTTTGTGAACCGTTTCATCGAAGCACTTTTTTACTTTAGTGATGAGAAGAACAGGGTGGTTTTAGAATCAAAGTATTTTGGTCATGGAATTAGGAATATTGTGGCTGAAGAAGGAGCTGAAAGAAAGAGTAGAAATGTAAAAATTGATGTGTGGAGAGCATTTTTCACAAGATTTGGAATGGTGGAAACAGAATTGAGCATGATGTCTTTGTATCAAGCTGAATTGATGGCTAAAAGATTTCCTTGTGGATGTTCTTGCACATTTGATATGAATGTTGAGGATAAACTTAGTTCATCCAATGATACATTTTGGGAAACCAAAGGGCATAATGAAATGAAAAATGTTCAATTTTCTGTAGCAGAAGATCTAGGAGACTATCATGGAATTGAGTCTCTTTGCTCTAATTTTGGTTTCTTTCAAGATGATCCATCACAAGAAGATGAGTTCCTAAAGTATCATCACCAACATCAACAAGAATCATATCAAGATTATGAAACTTTTGATAACCTACACTTTGACATGGTTCACTTTGATGAACAATGTCCTAAAAGAATTCTTCCACTTTGTGACACAAAAAAGGACCAACCATTCTACCAAACTCCTTTGGCGCCAGTTGACATATTGAAGAATTATGGCAAAGGATTCAAGAGGCTATTACCTGATGATGAAGGAAAAATACTTCATCCAGTTAATGACTTTGGTTTAGTCACAAATAATGAAAATGAGAGAAAGTTGTTGTCAACTATAGAGATCATGAAGCTAGCTGGAACAAGGTTCATACAATCATCATCTTCGTCGGAATCTGCATCAGGTTTGATTCTTAATCACCCTTTTGGTTTTTGTTTCTCTGGTTTATCTGATGAAGAGAAAGAAGATGTTTCACTTGCTGAATCACTCTTTGCTTGTGCTGAGAAAGTCGGATATCAACAATATGAACGCGCTAGAAAATTGTTATCACAAATCGAGTCTTTATCTTCGAAGACAGGTAACCCGGTTAAGCGCGTGGTTCATTATTTTGCTCAAGCTCTTCGCCAAAGAATCGACAAAGAAACAGGACGATTTTCTGTTTCATCCAACAATATGCAAAAGTTGGAATCTTTATTTGATCCTGAAGAGGTTAGCAAGGATCTAAACCCAGCCATGATCGCGCTTTACGAAGATCTTCCATTCAGTCAAGTTTCAATATTCACATGTGTACAAGCTTTATTGGAAAATGTAAATGATGCAAAGAAGATTCATGTTATTGATCTCGAAATTCGAAAAGGGTGTCAATGGACAATCCTAATGCAAGCACTTCAATCGAGAAACGAATGTCCACTCGAGCTTCTAAAGATAACAGCTATTGAGAGCGGAAACTCAGACACTTCGAAGCATATAGTTGAGGACACAGGTAAAAGATTGAAGGATTTTGCGCAAAGTTTGAACATACCTTTTTCGTTCGATATAGTTATTGTATCTGATTTGTTAAATTTAAGAGAAGATCTTTTCAAGATAGATTCGGAAGAAACAGTCGCGGTTTATTCTCAATTTGCGCTTAAAAGCAAGATTCAACAACCAGACAAACTCGAAACAATCATGAGAGTGGTTAGAACCATAAATCCTATAGTAATGGTTGTCGCCGAGATTGAAGCTAATCACAATTCCAAATCTTTTGTGAACCGTTTCATCGAAGCACTTTTTTACTTTAGTGCATACTTTGATTGTTTTGAGAATTGTATGAAAGGTGATGAGAAAAACAGATTTATTCTAGAGTCAAAGTATTTTAGTCATGGTATTAGGAATATTGTGGCTGAAGAAGGAGCTGAAAGAAAGAGTAGGAATGTAAAGATTGATGTTTGGAGAGCATTTTTCACAAGATTTGGAATGGTGGAAACAGAATTGAGTATGAAGTCTTTGTATCAAGCTGAATTGGTGGCTAAAAGATTTCCTTGTGGATGTTCTTGCACATTTGATATGAATGGTCATTGTCTTCTTATTGGATGGAAAGGAACACCAATTAACTCTGTTTCTGTTTGGAAATTCATTTGA

Protein sequence

>Msa0280530

MENLCNFGELSFNIVEDKLSSSNDIFWETKKLSEMKNVQFSVEEDLGDYNGTKSLCTNFDDPSQEDDFLKYHHQEQGSYQDYETFDNLHFDMVHFDEQQCPAKDPPLCDTKKDQQFYQTSLAPNYGKCFKRLLPDEGKTLHPVNDFGLITDNENESKLSTEDIMKIAGTRFIQSSSSSQSASGLILNHPFGFCFSGLSDEEKEDLVLRKSDINNMNELKNCYRKLSNPVKRVVHYFAEALQQRIDKETGRVSVSTNNMQKMGPLFDPQEVTKDLNPALVAFFEDLPFCKISMFTCVQTLIENIHVIDLEIRKGLQWTILMQALQLRNKCPLELLKITAIVTGNIDTSKHIVEDTGKRLKDFARYLKIPFLFDTIIVSDLEDLFKIDSEETIAVYSQFALQSKIQQSDQLETIMRVVRTINPIVMVVAEIEANHNSKSFVNRFIEALFYFSDEKNRVVLESKYFGHGIRNIVAEEGAERKSRNVKIDVWRAFFTRFGMVETELSMMSLYQAELMAKRFPCGCSCTFDMNVEDKLSSSNDTFWETKGHNEMKNVQFSVAEDLGDYHGIESLCSNFGFFQDDPSQEDEFLKYHHQHQQESYQDYETFDNLHFDMVHFDEQCPKRILPLCDTKKDQPFYQTPLAPVDILKNYGKGFKRLLPDDEGKILHPVNDFGLVTNNENERKLLSTIEIMKLAGTRFIQSSSSSESASGLILNHPFGFCFSGLSDEEKEDVSLAESLFACAEKVGYQQYERARKLLSQIESLSSKTGNPVKRVVHYFAQALRQRIDKETGRFSVSSNNMQKLESLFDPEEVSKDLNPAMIALYEDLPFSQVSIFTCVQALLENVNDAKKIHVIDLEIRKGCQWTILMQALQSRNECPLELLKITAIESGNSDTSKHIVEDTGKRLKDFAQSLNIPFSFDIVIVSDLLNLREDLFKIDSEETVAVYSQFALKSKIQQPDKLETIMRVVRTINPIVMVVAEIEANHNSKSFVNRFIEALFYFSAYFDCFENCMKGDEKNRFILESKYFSHGIRNIVAEEGAERKSRNVKIDVWRAFFTRFGMVETELSMKSLYQAELVAKRFPCGCSCTFDMNGHCLLIGWKGTPINSVSVWKFI*