AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0280810

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0280810 MtrunA17_Chr2g0294911 97.632 380 7 2 1 379 1 379 0.0 765
Msa0280810 MtrunA17_Chr4g0067981 70.874 412 91 7 1 386 2 410 0.0 545
Msa0280810 MtrunA17_Chr2g0294861 96.279 215 8 0 379 593 50 264 1.68e-151 436
Msa0280810 MtrunA17_Chr1g0161431 59.091 308 117 3 25 325 8 313 3.80e-116 358
Msa0280810 MtrunA17_Chr2g0294871 81.818 198 33 2 378 574 47 242 9.39e-111 331
Msa0280810 MtrunA17_Chr3g0137101 48.493 365 142 9 26 357 6 357 1.20e-101 322
Msa0280810 MtrunA17_Chr3g0123131 58.687 259 91 3 28 276 13 265 4.18e-95 302
Msa0280810 MtrunA17_Chr8g0376881 58.103 253 104 1 26 276 23 275 5.63e-92 293
Msa0280810 MtrunA17_Chr1g0149841 45.938 357 163 9 24 369 1 338 1.41e-90 288
Msa0280810 MtrunA17_Chr7g0275891 47.604 313 132 6 24 315 1 302 1.31e-85 270
Msa0280810 MtrunA17_Chr2g0304841 42.973 370 179 6 24 364 14 380 9.26e-85 278
Msa0280810 MtrunA17_Chr4g0074401 41.333 375 167 7 22 362 11 366 1.81e-81 267
Msa0280810 MtrunA17_Chr1g0149851 52.045 269 110 3 24 273 1 269 3.97e-79 259
Msa0280810 MtrunA17_Chr4g0074411 41.823 373 165 9 22 362 12 364 1.67e-78 259
Msa0280810 MtrunA17_Chr7g0275401 47.751 289 139 4 24 303 1 286 1.38e-75 242
Msa0280810 MtrunA17_Chr4g0074421 51.515 198 73 3 78 264 2 187 1.38e-59 197
Msa0280810 MtrunA17_Chr3g0134461 47.034 236 76 2 25 259 5 192 1.57e-59 197
Msa0280810 MtrunA17_Chr3g0134481 43.882 237 75 2 25 261 5 183 5.22e-57 190
Msa0280810 MtrunA17_Chr1g0149831 50.500 200 84 3 87 278 1 193 2.20e-56 189
Msa0280810 MtrunA17_Chr4g0009851 43.096 239 112 5 28 266 11 225 3.05e-56 192
Msa0280810 MtrunA17_Chr4g0009901 42.917 240 113 4 28 267 10 225 3.97e-55 187
Msa0280810 MtrunA17_Chr6g0471041 39.344 244 116 7 27 266 13 228 7.21e-45 164
Msa0280810 MtrunA17_Chr4g0009981 41.753 194 90 1 73 266 2 172 6.84e-44 157
Msa0280810 MtrunA17_Chr8g0391911 33.955 268 133 6 28 272 9 255 1.02e-35 134
Msa0280810 MtrunA17_Chr6g0473731 38.000 200 89 7 108 298 10 183 3.43e-29 120
Msa0280810 MtrunA17_Chr8g0383961 42.754 138 76 1 27 164 13 147 1.57e-28 114
Msa0280810 MtrunA17_Chr8g0350761 29.630 270 166 5 30 277 19 286 1.22e-27 116
Msa0280810 MtrunA17_Chr4g0011071 34.969 163 83 3 74 236 35 174 7.93e-27 107
Msa0280810 MtrunA17_Chr4g0031961 50.909 110 45 3 186 286 99 208 4.37e-24 103
Msa0280810 MtrunA17_Chr4g0009991 39.286 140 61 2 120 259 1 116 4.21e-22 92.0
Msa0280810 MtrunA17_Chr6g0456631 36.220 127 78 2 26 150 7 132 1.24e-21 92.4
Msa0280810 MtrunA17_Chr3g0127941 36.875 160 94 4 32 186 56 213 3.50e-21 97.8
Msa0280810 MtrunA17_Chr7g0255711 57.971 69 29 0 203 271 84 152 4.69e-21 94.0
Msa0280810 MtrunA17_Chr7g0276361 38.971 136 74 2 29 158 11 143 1.09e-20 96.3
Msa0280810 MtrunA17_Chr4g0030271 39.024 123 70 2 32 150 89 210 3.28e-20 95.1
Msa0280810 MtrunA17_Chr3g0091641 35.971 139 84 2 19 152 9 147 1.30e-19 92.8
Msa0280810 MtrunA17_Chr1g0181811 39.344 122 71 1 32 150 85 206 1.62e-19 92.8
Msa0280810 MtrunA17_Chr4g0061021 35.507 138 84 2 18 152 21 156 4.11e-18 88.2
Msa0280810 MtrunA17_Chr8g0345901 39.344 122 67 3 34 150 38 157 6.34e-18 87.4
Msa0280810 MtrunA17_Chr5g0400241 37.903 124 68 2 145 268 255 369 4.33e-17 84.7
Msa0280810 MtrunA17_Chr5g0400241 37.500 128 74 3 28 153 14 137 1.29e-13 73.6
Msa0280810 MtrunA17_Chr5g0423031 45.679 81 43 1 28 108 10 89 6.36e-17 79.3
Msa0280810 MtrunA17_Chr1g0198601 37.209 129 76 3 198 325 199 323 3.39e-16 80.9
Msa0280810 MtrunA17_Chr4g0010001 44.444 81 44 1 28 108 10 89 4.76e-16 73.6
Msa0280810 MtrunA17_Chr5g0417521 55.714 70 24 2 206 274 11 74 4.31e-15 77.0
Msa0280810 MtrunA17_Chr4g0054981 47.887 71 37 0 209 279 309 379 6.64e-15 77.8
Msa0280810 MtrunA17_Chr4g0054981 36.220 127 77 2 28 153 14 137 2.81e-13 72.8
Msa0280810 MtrunA17_Chr4g0040481 41.379 87 46 1 209 295 157 238 7.56e-15 77.0
Msa0280810 MtrunA17_Chr6g0469321 63.462 52 19 0 227 278 71 122 2.83e-14 71.2
Msa0280810 MtrunA17_Chr2g0324891 53.333 60 28 0 209 268 160 219 6.55e-14 73.9
Msa0280810 MtrunA17_Chr4g0042451 55.932 59 25 1 206 263 13 71 8.35e-14 73.2
Msa0280810 MtrunA17_Chr5g0422621 30.220 182 115 5 151 325 133 309 9.88e-14 73.2
Msa0280810 MtrunA17_Chr2g0321461 41.748 103 49 4 206 300 41 140 1.17e-13 73.2
Msa0280810 MtrunA17_Chr6g0461641 52.542 59 27 1 206 263 38 96 2.62e-13 72.0
Msa0280810 MtrunA17_Chr5g0402631 50.000 72 35 1 207 277 211 282 4.53e-13 70.9
Msa0280810 MtrunA17_Chr4g0045361 50.746 67 32 1 207 272 271 337 1.43e-12 69.7
Msa0280810 MtrunA17_Chr2g0310011 54.237 59 26 1 206 263 31 89 3.72e-12 68.2
Msa0280810 MtrunA17_Chr8g0356501 54.237 59 26 1 206 263 33 91 4.40e-12 67.8
Msa0280810 MtrunA17_Chr4g0062921 36.283 113 66 3 166 273 188 299 6.57e-12 67.8
Msa0280810 MtrunA17_Chr1g0197171 52.308 65 27 2 203 263 34 98 8.99e-12 66.2
Msa0280810 MtrunA17_Chr7g0274471 59.259 54 21 1 210 262 218 271 1.45e-11 67.0
Msa0280810 MtrunA17_Chr7g0261911 49.206 63 31 1 206 267 258 320 3.97e-11 65.5
Msa0280810 MtrunA17_Chr1g0190301 50.847 59 28 1 205 262 170 228 7.75e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0280810 AT3G16857.1 60.779 385 104 9 21 358 26 410 1.87e-152 454
Msa0280810 AT3G16857.2 60.779 385 104 9 21 358 26 410 8.09e-152 453
Msa0280810 AT3G16857.3 58.235 340 95 9 66 358 1 340 6.60e-122 374
Msa0280810 AT2G01760.1 45.361 388 167 9 24 382 3 374 2.09e-96 300
Msa0280810 AT2G25180.1 59.924 262 93 3 26 281 11 266 3.40e-96 306
Msa0280810 AT2G01760.2 41.587 416 170 9 24 382 3 402 2.54e-90 285
Msa0280810 AT4G31920.1 45.938 357 137 9 24 355 9 334 1.59e-87 282
Msa0280810 AT1G67710.1 49.704 338 125 10 29 333 8 333 1.21e-85 276
Msa0280810 AT5G58080.1 44.886 352 147 8 26 351 12 342 5.88e-80 265
Msa0280810 AT5G49320.1 61.053 190 68 4 390 577 59 244 4.02e-73 235
Msa0280810 AT2G01760.3 41.538 325 145 9 87 382 1 309 1.10e-62 209
Msa0280810 AT1G67710.2 45.714 280 107 10 87 333 1 268 1.96e-56 197
Msa0280810 AT5G49240.1 42.915 247 134 3 18 259 28 272 5.32e-54 186
Msa0280810 AT1G49190.1 36.364 275 134 6 27 277 29 286 4.36e-46 167
Msa0280810 AT3G62670.1 37.302 252 143 5 33 278 42 284 1.62e-45 167
Msa0280810 AT1G67710.3 41.538 260 107 10 107 333 12 259 1.61e-40 153
Msa0280810 AT5G07210.2 33.955 268 145 6 32 269 16 281 1.58e-38 150
Msa0280810 AT5G07210.1 32.971 276 145 7 32 269 16 289 4.81e-36 143
Msa0280810 AT2G27070.1 32.103 271 147 7 32 268 16 283 1.30e-32 132
Msa0280810 AT1G49190.2 44.800 125 69 0 27 151 29 153 5.88e-29 122
Msa0280810 AT5G59570.2 52.083 96 43 1 194 286 125 220 5.25e-25 105
Msa0280810 AT5G59570.1 52.083 96 43 1 194 286 125 220 5.25e-25 105
Msa0280810 AT3G46640.2 51.064 94 45 1 194 286 128 221 3.23e-23 101
Msa0280810 AT3G46640.1 51.064 94 45 1 194 286 128 221 3.23e-23 101
Msa0280810 AT3G46640.3 51.064 94 45 1 194 286 128 221 3.61e-23 101
Msa0280810 AT2G46790.3 40.650 123 68 2 32 150 37 158 4.83e-22 99.8
Msa0280810 AT2G46790.1 40.650 123 68 2 32 150 37 158 5.04e-22 99.8
Msa0280810 AT2G40970.1 60.606 66 26 0 203 268 98 163 1.13e-20 92.0
Msa0280810 AT5G24470.1 39.200 125 71 2 31 151 49 172 3.56e-20 94.7
Msa0280810 AT5G05090.1 59.091 66 27 0 203 268 74 139 3.66e-20 91.3
Msa0280810 AT5G60100.7 39.837 123 69 2 32 150 64 185 4.86e-20 93.2
Msa0280810 AT5G60100.6 39.837 123 69 2 32 150 64 185 4.86e-20 93.2
Msa0280810 AT5G60100.4 39.837 123 69 2 32 150 64 185 4.86e-20 93.2
Msa0280810 AT3G10760.1 59.091 66 27 0 203 268 98 163 6.81e-20 91.7
Msa0280810 AT5G60100.5 39.837 123 69 2 32 150 64 185 9.08e-20 93.2
Msa0280810 AT5G60100.3 39.837 123 69 2 32 150 64 185 9.08e-20 93.2
Msa0280810 AT5G60100.1 39.837 123 69 2 32 150 64 185 9.08e-20 93.2
Msa0280810 AT5G60100.2 39.837 123 69 2 32 150 64 185 1.11e-19 93.2
Msa0280810 AT5G61380.1 38.710 124 73 1 32 152 19 142 4.95e-19 91.7
Msa0280810 AT5G02810.1 40.164 122 70 1 32 150 78 199 8.54e-19 90.9
Msa0280810 AT5G44190.2 50.649 77 38 0 203 279 140 216 7.58e-18 85.9
Msa0280810 AT5G44190.1 50.649 77 38 0 203 279 140 216 1.16e-17 85.9
Msa0280810 AT2G20570.1 39.823 113 63 2 170 277 108 220 6.13e-16 80.9
Msa0280810 AT2G20570.2 51.515 66 32 0 212 277 171 236 2.93e-14 75.9
Msa0280810 AT3G04030.1 51.282 78 30 3 206 282 41 111 3.13e-14 75.1
Msa0280810 AT3G04030.3 51.282 78 30 3 206 282 41 111 3.56e-14 75.1
Msa0280810 AT5G18240.2 50.617 81 36 3 206 285 41 118 5.44e-14 74.7
Msa0280810 AT5G18240.3 50.617 81 36 3 206 285 41 118 5.44e-14 74.7
Msa0280810 AT5G18240.5 50.617 81 36 3 206 285 41 118 6.20e-14 74.3
Msa0280810 AT5G18240.1 50.617 81 36 3 206 285 41 118 6.39e-14 74.3
Msa0280810 AT5G18240.4 50.617 81 36 3 206 285 41 118 6.39e-14 74.3
Msa0280810 AT3G04030.2 48.052 77 33 2 206 282 41 110 3.91e-13 72.0
Msa0280810 AT4G18020.8 51.613 62 29 1 207 268 294 354 4.46e-13 72.4
Msa0280810 AT4G18020.8 36.029 136 78 3 26 154 12 145 2.07e-12 70.1
Msa0280810 AT4G18020.11 51.613 62 29 1 207 268 294 354 4.46e-13 72.4
Msa0280810 AT4G18020.11 36.029 136 78 3 26 154 12 145 2.07e-12 70.1
Msa0280810 AT4G18020.4 51.613 62 29 1 207 268 294 354 4.46e-13 72.4
Msa0280810 AT4G18020.4 36.029 136 78 3 26 154 12 145 2.07e-12 70.1
Msa0280810 AT4G18020.5 51.613 62 29 1 207 268 294 354 4.46e-13 72.4
Msa0280810 AT4G18020.5 36.029 136 78 3 26 154 12 145 2.07e-12 70.1
Msa0280810 AT4G18020.10 51.613 62 29 1 207 268 294 354 4.46e-13 72.4
Msa0280810 AT4G18020.10 36.029 136 78 3 26 154 12 145 2.07e-12 70.1
Msa0280810 AT4G18020.7 51.613 62 29 1 207 268 294 354 4.46e-13 72.4
Msa0280810 AT4G18020.7 36.029 136 78 3 26 154 12 145 2.07e-12 70.1
Msa0280810 AT4G18020.3 51.613 62 29 1 207 268 294 354 4.93e-13 72.4
Msa0280810 AT4G18020.3 36.029 136 78 3 26 154 12 145 2.81e-12 69.7
Msa0280810 AT4G18020.2 51.613 62 29 1 207 268 294 354 4.93e-13 72.4
Msa0280810 AT4G18020.2 36.029 136 78 3 26 154 12 145 2.81e-12 69.7
Msa0280810 AT4G18020.1 51.613 62 29 1 207 268 294 354 4.93e-13 72.4
Msa0280810 AT4G18020.1 36.029 136 78 3 26 154 12 145 2.81e-12 69.7
Msa0280810 AT4G18020.6 51.613 62 29 1 207 268 294 354 4.93e-13 72.4
Msa0280810 AT4G18020.6 36.029 136 78 3 26 154 12 145 2.81e-12 69.7
Msa0280810 AT4G18020.9 51.613 62 29 1 207 268 294 354 4.93e-13 72.4
Msa0280810 AT4G18020.9 36.029 136 78 3 26 154 12 145 2.81e-12 69.7
Msa0280810 AT1G69580.1 54.237 59 26 1 206 263 30 88 7.87e-13 70.5
Msa0280810 AT1G69580.2 54.237 59 26 1 206 263 30 88 8.01e-13 70.5
Msa0280810 AT3G24120.1 52.308 65 27 2 203 263 31 95 4.48e-12 67.8
Msa0280810 AT3G24120.2 52.308 65 27 2 203 263 31 95 4.49e-12 67.8
Msa0280810 AT1G49560.1 37.383 107 59 2 209 307 191 297 6.97e-12 67.4
Msa0280810 AT1G79430.2 54.237 59 26 1 206 263 30 88 7.19e-12 67.8
Msa0280810 AT4G13640.1 52.308 65 27 2 203 263 27 91 7.61e-12 67.0
Msa0280810 AT4G13640.2 52.308 65 27 2 203 263 27 91 7.70e-12 67.0
Msa0280810 AT3G25790.1 37.719 114 59 4 214 325 200 303 9.79e-12 67.4
Msa0280810 AT1G68670.1 45.679 81 42 2 194 272 198 278 1.32e-11 67.0
Msa0280810 AT1G25550.1 45.070 71 38 1 198 267 197 267 1.53e-11 66.6
Msa0280810 AT3G12730.1 49.275 69 28 2 202 263 9 77 2.53e-11 64.3
Msa0280810 AT2G03500.1 47.059 68 35 1 207 273 230 297 5.27e-11 65.5
Msa0280810 AT5G29000.4 45.714 70 35 2 196 262 215 284 6.02e-11 64.3

Find 123 sgRNAs with CRISPR-Local

Find 226 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCACCTTCATTAATTAAT+TGG 0.068072 2_3:+15861639 Msa0280810:CDS
TGAGCTTCATCAACAGTTTA+TGG 0.134096 2_3:+15860039 Msa0280810:CDS
CTTCAAACCCTTTCAGCTTC+TGG 0.197138 2_3:+15860924 Msa0280810:CDS
CCTTATCTCCGACCAGTTTC+CGG 0.220153 2_3:+15859001 Msa0280810:CDS
GTTGTTGTTGTTGAGTTTCA+TGG 0.237423 2_3:-15858934 None:intergenic
ATGCTGTAGCAATTTGTTCT+TGG 0.243620 2_3:-15862012 None:intergenic
CATGGATGGATTCAAACTTT+TGG 0.246707 2_3:+15859554 Msa0280810:CDS
CTTTGACTTTGAGGTGATCT+TGG 0.295999 2_3:-15862195 None:intergenic
ATTAAACTAAGAAAATTAAT+TGG 0.296217 2_3:-15858802 None:intergenic
TGCAAGTTACAACTGTTATA+AGG 0.306416 2_3:+15858744 Msa0280810:five_prime_UTR
AGTACGCCAGAGTCAATATT+TGG 0.308653 2_3:+15860876 Msa0280810:CDS
CACCAGTCTGCTCAATCCTT+TGG 0.321223 2_3:+15861122 Msa0280810:CDS
TTGAAGATCAATTTCATTTA+CGG 0.326626 2_3:-15860907 None:intergenic
GAGAACTCAAAACTAAGATT+TGG 0.328580 2_3:+15861002 Msa0280810:CDS
ACTAGCAATTGCTTTGGTTA+TGG 0.333310 2_3:+15861583 Msa0280810:CDS
AAGAAGATGAAGCAGATGAT+AGG 0.337750 2_3:+15859972 Msa0280810:CDS
AAGAAAATTAATTGGTTTGT+GGG 0.341269 2_3:-15858794 None:intergenic
TGCTTTGGCTCAATGTTGAT+AGG 0.342601 2_3:-15861086 None:intergenic
ATCACCTTCATTAATTAATT+GGG 0.347636 2_3:+15861640 Msa0280810:CDS
TTGAGCAGACTGGTGAAAAT+TGG 0.352834 2_3:-15861114 None:intergenic
TTCAGCTTCTGGCCAGTTCT+CGG 0.367341 2_3:+15860935 Msa0280810:CDS
TGAAAATTGGCAAACTGCTT+TGG 0.368522 2_3:-15861101 None:intergenic
TCGAGGCTTTGAAGAATATA+TGG 0.369544 2_3:+15859798 Msa0280810:CDS
AAACAATGCTTACTAATAAC+AGG 0.383212 2_3:-15859603 None:intergenic
TAAGAAAATTAATTGGTTTG+TGG 0.396703 2_3:-15858795 None:intergenic
TTCGGAAGAGAAAGAATAAC+TGG 0.397525 2_3:+15859831 Msa0280810:CDS
GATTTAGCAGAAGGAGAATC+AGG 0.397735 2_3:-15861665 None:intergenic
CTAGACTTTGCGCCGAGAAC+TGG 0.398375 2_3:-15860947 None:intergenic
CACGGAGGCCCGCCGGAAAC+TGG 0.401569 2_3:-15859013 None:intergenic
AAACGTTGCCAGTCATCTTC+AGG 0.402049 2_3:+15860413 Msa0280810:CDS
GCAATATGTCTTTGACTTTG+AGG 0.410164 2_3:-15862204 None:intergenic
CAAAGAAGCCTCGAGTTGTT+TGG 0.412556 2_3:+15860011 Msa0280810:CDS
AACCTCAGCTCTCTTACATT+TGG 0.414035 2_3:-15859463 None:intergenic
TGTTGGACTAGCAATTGCTT+TGG 0.417090 2_3:+15861577 Msa0280810:CDS
TATTCAAGTTACTTTGCTGC+TGG 0.418842 2_3:-15860842 None:intergenic
GAAGGTTTGTTCTCAGCTGC+AGG 0.422199 2_3:+15862039 Msa0280810:CDS
AATGTGTATACAATAGTAAT+GGG 0.423786 2_3:+15861522 Msa0280810:CDS
ATGGAAATGATGAATGTTCC+TGG 0.426157 2_3:+15860378 Msa0280810:CDS
GAATACAAATGGAAAAGAAA+AGG 0.430139 2_3:+15861487 Msa0280810:CDS
AAAACCAGCCAACCATGTTA+AGG 0.443331 2_3:-15861687 None:intergenic
AAGATTGAAGACCTTCATAA+AGG 0.449070 2_3:-15859097 None:intergenic
TTTGTGCACGAAGTGCTGTT+AGG 0.449903 2_3:-15862104 None:intergenic
TTTGACTCTCAATTGTTGTA+GGG 0.459330 2_3:-15862245 None:intergenic
ATAAGGTTGCCGGAGTTTGA+TGG 0.460200 2_3:-15858984 None:intergenic
TATGGCTGTTGTGAATCAAT+TGG 0.460490 2_3:+15860057 Msa0280810:CDS
TGCTAAATCCTTAACATGGT+TGG 0.463393 2_3:+15861679 Msa0280810:CDS
TGGAAAGATCTGAAGCAATC+TGG 0.465336 2_3:+15859851 Msa0280810:CDS
CCGGAAACTGGTCGGAGATA+AGG 0.470882 2_3:-15859001 None:intergenic
ATGGCTGTTGTGAATCAATT+GGG 0.473696 2_3:+15860058 Msa0280810:CDS
GAATGTGTATACAATAGTAA+TGG 0.474577 2_3:+15861521 Msa0280810:CDS
TATGAGCAGCAGCAGTAAAC+CGG 0.476502 2_3:+15861043 Msa0280810:CDS
CTAGCCAAGCTTCAAGCAGC+AGG 0.477941 2_3:+15860966 Msa0280810:CDS
AAATCCTTAACATGGTTGGC+TGG 0.479778 2_3:+15861683 Msa0280810:CDS
GGAACATATTGGATTGGAGA+TGG 0.480393 2_3:+15859575 Msa0280810:CDS
AGAAGATGAAGCAGATGATA+GGG 0.481453 2_3:+15859973 Msa0280810:CDS
TGACTTTGAGGTGATCTTGG+TGG 0.482477 2_3:-15862192 None:intergenic
GTGGCATGGATGAATACAAA+TGG 0.489952 2_3:+15861476 Msa0280810:CDS
TACTCATCTTCAACGAATGA+AGG 0.499328 2_3:+15859923 Msa0280810:CDS
GCTTTGGCTCAATGTTGATA+GGG 0.500235 2_3:-15861085 None:intergenic
GCAGGACTTAGTGTAGAATC+AGG 0.501466 2_3:+15861967 Msa0280810:CDS
CAAAACTAAGATTTGGAGAT+GGG 0.507173 2_3:+15861009 Msa0280810:CDS
CAAGGATCATGAGGCAAGTA+GGG 0.508406 2_3:-15859055 None:intergenic
CTGGTCGGAGATAAGGTTGC+CGG 0.509887 2_3:-15858994 None:intergenic
ATTCAAGTTACTTTGCTGCT+GGG 0.515195 2_3:-15860841 None:intergenic
TTCAAAGCCTCGATTCGCAC+AGG 0.523205 2_3:-15859788 None:intergenic
ATATATGGCAGCATGTGGTT+CGG 0.524503 2_3:+15859813 Msa0280810:CDS
TCAAGGATCATGAGGCAAGT+AGG 0.525643 2_3:-15859056 None:intergenic
AATACAAATGGAAAAGAAAA+GGG 0.525657 2_3:+15861488 Msa0280810:CDS
TAGGATTTGTTTCGCAGTTG+CGG 0.527627 2_3:+15861930 Msa0280810:intron
TTTAAGCGATGTGCATATGC+CGG 0.529451 2_3:+15859530 Msa0280810:CDS
CATGTTAAGGATTTAGCAGA+AGG 0.532122 2_3:-15861674 None:intergenic
AAACCGGTGAACTTTCTTCA+CGG 0.535622 2_3:+15861059 Msa0280810:CDS
CGATGTGCATATGCCGGACA+TGG 0.535790 2_3:+15859536 Msa0280810:CDS
TCAAAACTAAGATTTGGAGA+TGG 0.536886 2_3:+15861008 Msa0280810:CDS
ATCTCCGACCAGTTTCCGGC+GGG 0.537771 2_3:+15859005 Msa0280810:CDS
TTGTATCTTCGAAGGTTGAG+TGG 0.537963 2_3:+15860813 Msa0280810:CDS
GGATCCCAATTAATTAATGA+AGG 0.538415 2_3:-15861644 None:intergenic
GAACTGGCCAGAAGCTGAAA+GGG 0.541735 2_3:-15860931 None:intergenic
AGTTTGAATCCATCCATGTC+CGG 0.543637 2_3:-15859549 None:intergenic
TCGCATATTTGCGCTGCCAA+AGG 0.548078 2_3:-15861138 None:intergenic
AATACCGTTTGTATCTTCGA+AGG 0.548651 2_3:+15860805 Msa0280810:CDS
GCACAAGCAGCACATAACAA+AGG 0.553637 2_3:-15861551 None:intergenic
GTGCATATGCCGGACATGGA+TGG 0.559547 2_3:+15859540 Msa0280810:CDS
ATATCATACCTGAAGATGAC+TGG 0.562314 2_3:-15860421 None:intergenic
AAGGTTTGTTCTCAGCTGCA+GGG 0.562389 2_3:+15862040 Msa0280810:CDS
AGAACTGGCCAGAAGCTGAA+AGG 0.565004 2_3:-15860932 None:intergenic
ATAGTGATGTCGGCTGATGA+TGG 0.565866 2_3:+15859710 Msa0280810:intron
ACAAGTACACGGAGGCCCGC+CGG 0.571204 2_3:-15859020 None:intergenic
ATTCCGTGAAGAAAGTTCAC+CGG 0.576781 2_3:-15861062 None:intergenic
GTTCCTCCAAATATTGACTC+TGG 0.578483 2_3:-15860882 None:intergenic
AGGTTTGTTCTCAGCTGCAG+GGG 0.579049 2_3:+15862041 Msa0280810:CDS
TGCCAAAGGATTGAGCAGAC+TGG 0.579512 2_3:-15861124 None:intergenic
TGACTCTGGCGTACTCATGA+AGG 0.583909 2_3:-15860868 None:intergenic
GCAAGAGCTCAAAGAAGAGG+AGG 0.587165 2_3:+15859945 Msa0280810:CDS
AAGTCCTGCTGCTTGAAGCT+TGG 0.592461 2_3:-15860970 None:intergenic
GAATGTGTTTATAGTGATGT+CGG 0.592942 2_3:+15859700 Msa0280810:intron
AGATGAGTAATTAGCATCAG+TGG 0.594629 2_3:-15859909 None:intergenic
TGGTGAAGTTGAGGTTGACA+TGG 0.599570 2_3:-15858964 None:intergenic
TACTCTACAAATTTATACAA+AGG 0.601260 2_3:-15862319 None:intergenic
CTTCTGCTAAATCCTTAACA+TGG 0.602252 2_3:+15861675 Msa0280810:CDS
AATTGCTACAGCATCAGAGA+AGG 0.604858 2_3:+15862021 Msa0280810:CDS
AAGATCAATTTCATTTACGG+AGG 0.607064 2_3:-15860904 None:intergenic
TTAGGTATAAACCAGCAGCT+AGG 0.613595 2_3:-15862086 None:intergenic
TCAACCTTCGAAGATACAAA+CGG 0.613662 2_3:-15860809 None:intergenic
AATCAAACCTGTGCGAATCG+AGG 0.614923 2_3:+15859781 Msa0280810:CDS
GTTTGAAATGATAGGTGGCA+TGG 0.616555 2_3:+15861462 Msa0280810:intron
TCAACTTCACCATCAAACTC+CGG 0.617812 2_3:+15858975 Msa0280810:CDS
TATCTCCGACCAGTTTCCGG+CGG 0.618522 2_3:+15859004 Msa0280810:CDS
GCATTCTTTCAAGGATCATG+AGG 0.625199 2_3:-15859064 None:intergenic
TGGAAATGATGAATGTTCCT+GGG 0.628797 2_3:+15860379 Msa0280810:CDS
AAAGATCTGAAGCAATCTGG+TGG 0.628824 2_3:+15859854 Msa0280810:CDS
GAAGAATATATGGCAGCATG+TGG 0.629609 2_3:+15859808 Msa0280810:CDS
AAGGCAAGAGCTCAAAGAAG+AGG 0.635070 2_3:+15859942 Msa0280810:CDS
ACGCCAGAGTCAATATTTGG+AGG 0.638077 2_3:+15860879 Msa0280810:CDS
TCAATAGACCAAACAACTCG+AGG 0.639848 2_3:-15860019 None:intergenic
GAGGCCCGCCGGAAACTGGT+CGG 0.643720 2_3:-15859009 None:intergenic
TCGTGCACAAAGAATGTCTG+AGG 0.648802 2_3:+15862116 Msa0280810:CDS
GGAGTTTGATGGTGAAGTTG+AGG 0.651951 2_3:-15858973 None:intergenic
CATCATCAACAACAAGTACA+CGG 0.653506 2_3:-15859031 None:intergenic
CAAGAGCTCAAAGAAGAGGA+GGG 0.663100 2_3:+15859946 Msa0280810:CDS
TACCAAATGTAAGAGAGCTG+AGG 0.673543 2_3:+15859461 Msa0280810:CDS
GTAATGAAAGGTGTGACACA+CGG 0.719143 2_3:+15859743 Msa0280810:CDS
CATCAACAACAAGTACACGG+AGG 0.818660 2_3:-15859028 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAAACTAAGAAAATTAAT+TGG - chr2_3:15858805-15858824 None:intergenic 10.0%
!!! TTTTATTTCTTTTACAATTT+CGG - chr2_3:15861797-15861816 None:intergenic 10.0%
!!! AGAAATATTTAAGATTCTAT+GGG - chr2_3:15860260-15860279 None:intergenic 15.0%
!!! CAATTTTTTGTAATAAATGA+AGG - chr2_3:15861762-15861781 None:intergenic 15.0%
!!! AGGTATAACTTTTATTAAAA+GGG - chr2_3:15862302-15862321 None:intergenic 15.0%
!!! AAGGTATAACTTTTATTAAA+AGG - chr2_3:15862303-15862322 None:intergenic 15.0%
!!! AAGAAAATTAATTGGTTTGT+GGG - chr2_3:15858797-15858816 None:intergenic 20.0%
!!! TAAGAAAATTAATTGGTTTG+TGG - chr2_3:15858798-15858817 None:intergenic 20.0%
!!! TTTTTTTGTTGTCTACTATT+TGG + chr2_3:15858902-15858921 Msa0280810:five_prime_UTR 20.0%
!!! TTTTAACACAAATTCATGAT+GGG - chr2_3:15859142-15859161 None:intergenic 20.0%
!!! TTTTTAACACAAATTCATGA+TGG - chr2_3:15859143-15859162 None:intergenic 20.0%
!! AAAAACTCAATCAAACAAAA+GGG - chr2_3:15859224-15859243 None:intergenic 20.0%
!! AAAAAACTCAATCAAACAAA+AGG - chr2_3:15859225-15859244 None:intergenic 20.0%
!! CCAGAACTAAAAATAAAAAT+GGG - chr2_3:15859256-15859275 None:intergenic 20.0%
!! ACCAGAACTAAAAATAAAAA+TGG - chr2_3:15859257-15859276 None:intergenic 20.0%
!!! TAAGTGTTTTTTTCAACTAT+TGG + chr2_3:15859338-15859357 Msa0280810:intron 20.0%
!!! TTTGATTATGTTTTGATTGT+TGG + chr2_3:15859422-15859441 Msa0280810:intron 20.0%
!!! TTGATTATGTTTTGATTGTT+GGG + chr2_3:15859423-15859442 Msa0280810:intron 20.0%
!! TTCTTAGAGAGAATAAAAAT+GGG + chr2_3:15859496-15859515 Msa0280810:CDS 20.0%
!!! GCATTGTTTTTTTATTGATT+TGG + chr2_3:15859617-15859636 Msa0280810:intron 20.0%
!!! AAATATTTAAGATTCTATGG+GGG - chr2_3:15860258-15860277 None:intergenic 20.0%
!!! GAAATATTTAAGATTCTATG+GGG - chr2_3:15860259-15860278 None:intergenic 20.0%
!!! CAGAAATATTTAAGATTCTA+TGG - chr2_3:15860261-15860280 None:intergenic 20.0%
!!! ATCATTTCCATAATCTTTTT+AGG - chr2_3:15860369-15860388 None:intergenic 20.0%
!! CTTCTAAACTAACTTATTAT+AGG - chr2_3:15860512-15860531 None:intergenic 20.0%
!!! ATAATAAGTTAGTTTAGAAG+TGG + chr2_3:15860512-15860531 Msa0280810:intron 20.0%
!! TTGAAGATCAATTTCATTTA+CGG - chr2_3:15860910-15860929 None:intergenic 20.0%
!! TCATGAAGTTTAATGTAATT+TGG + chr2_3:15861436-15861455 Msa0280810:intron 20.0%
!! AATACAAATGGAAAAGAAAA+GGG + chr2_3:15861488-15861507 Msa0280810:CDS 20.0%
!! AATGTGTATACAATAGTAAT+GGG + chr2_3:15861522-15861541 Msa0280810:CDS 20.0%
!! ATCACCTTCATTAATTAATT+GGG + chr2_3:15861640-15861659 Msa0280810:CDS 20.0%
!!! ACGTTTTTTTTTTATGTTGT+AGG + chr2_3:15861911-15861930 Msa0280810:intron 20.0%
!! AATCAGGTCATTTAAAAAAT+TGG + chr2_3:15861983-15862002 Msa0280810:CDS 20.0%
!!! GGTATAACTTTTATTAAAAG+GGG - chr2_3:15862301-15862320 None:intergenic 20.0%
!! AGTGAAAAGTGATGATTTTA+TGG - chr2_3:15858846-15858865 None:intergenic 25.0%
!!! CCCATTTTTATTTTTAGTTC+TGG + chr2_3:15859253-15859272 Msa0280810:intron 25.0%
!! GCTTTTGATATGATTTAGTA+TGG + chr2_3:15859302-15859321 Msa0280810:intron 25.0%
! CTTCTTAGAGAGAATAAAAA+TGG + chr2_3:15859495-15859514 Msa0280810:CDS 25.0%
!!! TTCAAACTTTTGGAACATAT+TGG + chr2_3:15859564-15859583 Msa0280810:CDS 25.0%
! AAACAATGCTTACTAATAAC+AGG - chr2_3:15859606-15859625 None:intergenic 25.0%
!!! TATTGATTTGGATCAATTGA+TGG + chr2_3:15859629-15859648 Msa0280810:intron 25.0%
! CAAGGACATAACATAATTAA+GGG - chr2_3:15860100-15860119 None:intergenic 25.0%
! ATATCTCGGAAAAACTTTAA+GGG - chr2_3:15860186-15860205 None:intergenic 25.0%
! TATATCTCGGAAAAACTTTA+AGG - chr2_3:15860187-15860206 None:intergenic 25.0%
! AAATTGATATTGATATGCAG+AGG + chr2_3:15860338-15860357 Msa0280810:intron 25.0%
! CTTAACAGTAACAAAAATAG+AGG - chr2_3:15860465-15860484 None:intergenic 25.0%
! TGAAGTTTAATGTAATTTGG+TGG + chr2_3:15861439-15861458 Msa0280810:intron 25.0%
! GAAGTTTAATGTAATTTGGT+GGG + chr2_3:15861440-15861459 Msa0280810:intron 25.0%
! GAATACAAATGGAAAAGAAA+AGG + chr2_3:15861487-15861506 Msa0280810:CDS 25.0%
! GAATGTGTATACAATAGTAA+TGG + chr2_3:15861521-15861540 Msa0280810:CDS 25.0%
! CATCACCTTCATTAATTAAT+TGG + chr2_3:15861639-15861658 Msa0280810:CDS 25.0%
!!! GTTTTTTCTTCATTACAACT+TGG + chr2_3:15861705-15861724 Msa0280810:CDS 25.0%
!! ATTTCTTTTACAATTTCGGA+TGG - chr2_3:15861793-15861812 None:intergenic 25.0%
!! TAACTTTTATTAAAAGGGGT+TGG - chr2_3:15862297-15862316 None:intergenic 25.0%
TGCAAGTTACAACTGTTATA+AGG + chr2_3:15858744-15858763 Msa0280810:five_prime_UTR 30.0%
AAGATTGAAGACCTTCATAA+AGG - chr2_3:15859100-15859119 None:intergenic 30.0%
! CAAGTCTCAAACTTTAACTT+TGG - chr2_3:15859372-15859391 None:intergenic 30.0%
!!! ACTTTTGGAACATATTGGAT+TGG + chr2_3:15859569-15859588 Msa0280810:CDS 30.0%
GAATGTGTTTATAGTGATGT+CGG + chr2_3:15859700-15859719 Msa0280810:intron 30.0%
GCAAGGACATAACATAATTA+AGG - chr2_3:15860101-15860120 None:intergenic 30.0%
! TGAAAGAGAACATTTTTGCA+AGG - chr2_3:15860118-15860137 None:intergenic 30.0%
!! TCATCTTTGTTTGTTTTCCA+CGG + chr2_3:15860135-15860154 Msa0280810:intron 30.0%
GAATCACAAGGATTATATCT+CGG - chr2_3:15860200-15860219 None:intergenic 30.0%
AACAATCTTGTAGAATCACA+AGG - chr2_3:15860212-15860231 None:intergenic 30.0%
! ATAAGTTAGTTTAGAAGTGG+TGG + chr2_3:15860515-15860534 Msa0280810:intron 30.0%
!! TCGTACAAACTGATTTTGTA+AGG + chr2_3:15860548-15860567 Msa0280810:intron 30.0%
!! CGTACAAACTGATTTTGTAA+GGG + chr2_3:15860549-15860568 Msa0280810:intron 30.0%
ACTGTAACTCTTAACATTCA+AGG - chr2_3:15860723-15860742 None:intergenic 30.0%
AAGATCAATTTCATTTACGG+AGG - chr2_3:15860907-15860926 None:intergenic 30.0%
!!! AATCTTAGTTTTGAGTTCTC+AGG - chr2_3:15861002-15861021 None:intergenic 30.0%
GAGAACTCAAAACTAAGATT+TGG + chr2_3:15861002-15861021 Msa0280810:CDS 30.0%
TCAAAACTAAGATTTGGAGA+TGG + chr2_3:15861008-15861027 Msa0280810:CDS 30.0%
CAAAACTAAGATTTGGAGAT+GGG + chr2_3:15861009-15861028 Msa0280810:CDS 30.0%
TAACACAGACCACTAATATT+CGG + chr2_3:15861324-15861343 Msa0280810:intron 30.0%
GGATCCCAATTAATTAATGA+AGG - chr2_3:15861647-15861666 None:intergenic 30.0%
TTTGACTCTCAATTGTTGTA+GGG - chr2_3:15862248-15862267 None:intergenic 30.0%
!! TTTTGACTCTCAATTGTTGT+AGG - chr2_3:15862249-15862268 None:intergenic 30.0%
! GTTGTTGTTGTTGAGTTTCA+TGG - chr2_3:15858937-15858956 None:intergenic 35.0%
CATCATCAACAACAAGTACA+CGG - chr2_3:15859034-15859053 None:intergenic 35.0%
! AAAAACGGAGCATTCTTTCA+AGG - chr2_3:15859076-15859095 None:intergenic 35.0%
GACCTTCATAAAGGCAAAAA+CGG - chr2_3:15859091-15859110 None:intergenic 35.0%
!! CATGGATGGATTCAAACTTT+TGG + chr2_3:15859554-15859573 Msa0280810:CDS 35.0%
! GGAAAAAACGTGGTAATGAA+AGG + chr2_3:15859731-15859750 Msa0280810:CDS 35.0%
!! TCGAGGCTTTGAAGAATATA+TGG + chr2_3:15859798-15859817 Msa0280810:CDS 35.0%
TTCGGAAGAGAAAGAATAAC+TGG + chr2_3:15859831-15859850 Msa0280810:CDS 35.0%
AGATGAGTAATTAGCATCAG+TGG - chr2_3:15859912-15859931 None:intergenic 35.0%
TACTCATCTTCAACGAATGA+AGG + chr2_3:15859923-15859942 Msa0280810:CDS 35.0%
AAGAAGATGAAGCAGATGAT+AGG + chr2_3:15859972-15859991 Msa0280810:CDS 35.0%
AGAAGATGAAGCAGATGATA+GGG + chr2_3:15859973-15859992 Msa0280810:CDS 35.0%
TGAGCTTCATCAACAGTTTA+TGG + chr2_3:15860039-15860058 Msa0280810:CDS 35.0%
TATGGCTGTTGTGAATCAAT+TGG + chr2_3:15860057-15860076 Msa0280810:CDS 35.0%
ATGGCTGTTGTGAATCAATT+GGG + chr2_3:15860058-15860077 Msa0280810:CDS 35.0%
! TCGGAAAAACTTTAAGGGAA+TGG - chr2_3:15860181-15860200 None:intergenic 35.0%
TAAGATTCTATGGGGGAAAA+GGG - chr2_3:15860251-15860270 None:intergenic 35.0%
TTAAGATTCTATGGGGGAAA+AGG - chr2_3:15860252-15860271 None:intergenic 35.0%
GGCTGTTCCTAAAAAGATTA+TGG + chr2_3:15860359-15860378 Msa0280810:CDS 35.0%
ATGGAAATGATGAATGTTCC+TGG + chr2_3:15860378-15860397 Msa0280810:CDS 35.0%
TGGAAATGATGAATGTTCCT+GGG + chr2_3:15860379-15860398 Msa0280810:CDS 35.0%
ATATCATACCTGAAGATGAC+TGG - chr2_3:15860424-15860443 None:intergenic 35.0%
CAAAATCAGTTTGTACGATG+AGG - chr2_3:15860546-15860565 None:intergenic 35.0%
AGAGCCTATATAAGATCTCA+TGG + chr2_3:15860600-15860619 Msa0280810:intron 35.0%
GAGCCTATATAAGATCTCAT+GGG + chr2_3:15860601-15860620 Msa0280810:intron 35.0%
GTAACTCTTAACATTCAAGG+TGG - chr2_3:15860720-15860739 None:intergenic 35.0%
AATACCGTTTGTATCTTCGA+AGG + chr2_3:15860805-15860824 Msa0280810:CDS 35.0%
TCAACCTTCGAAGATACAAA+CGG - chr2_3:15860812-15860831 None:intergenic 35.0%
! ATTCAAGTTACTTTGCTGCT+GGG - chr2_3:15860844-15860863 None:intergenic 35.0%
! TATTCAAGTTACTTTGCTGC+TGG - chr2_3:15860845-15860864 None:intergenic 35.0%
TGAAAATTGGCAAACTGCTT+TGG - chr2_3:15861104-15861123 None:intergenic 35.0%
TTTAACGAATTGAAGCGACA+TGG - chr2_3:15861351-15861370 None:intergenic 35.0%
AGTTACCATATACCACCAAA+AGG + chr2_3:15861392-15861411 Msa0280810:intron 35.0%
GTTACCATATACCACCAAAA+GGG + chr2_3:15861393-15861412 Msa0280810:intron 35.0%
!! TTTGGTGGGTTTGAAATGAT+AGG + chr2_3:15861454-15861473 Msa0280810:intron 35.0%
ACTAGCAATTGCTTTGGTTA+TGG + chr2_3:15861583-15861602 Msa0280810:CDS 35.0%
CATGTTAAGGATTTAGCAGA+AGG - chr2_3:15861677-15861696 None:intergenic 35.0%
CTTCTGCTAAATCCTTAACA+TGG + chr2_3:15861675-15861694 Msa0280810:CDS 35.0%
TGCTAAATCCTTAACATGGT+TGG + chr2_3:15861679-15861698 Msa0280810:CDS 35.0%
! TTTACAATTTCGGATGGACT+AGG - chr2_3:15861787-15861806 None:intergenic 35.0%
!! AGTTGCGGAGATTTTGTTAT+TGG + chr2_3:15861945-15861964 Msa0280810:CDS 35.0%
! ATGCTGTAGCAATTTGTTCT+TGG - chr2_3:15862015-15862034 None:intergenic 35.0%
! GCAATATGTCTTTGACTTTG+AGG - chr2_3:15862207-15862226 None:intergenic 35.0%
TCAACTTCACCATCAAACTC+CGG + chr2_3:15858975-15858994 Msa0280810:CDS 40.0%
GCATTCTTTCAAGGATCATG+AGG - chr2_3:15859067-15859086 None:intergenic 40.0%
!! CTCCGTTTTTGCCTTTATGA+AGG + chr2_3:15859086-15859105 Msa0280810:CDS 40.0%
TACCAAATGTAAGAGAGCTG+AGG + chr2_3:15859461-15859480 Msa0280810:CDS 40.0%
AACCTCAGCTCTCTTACATT+TGG - chr2_3:15859466-15859485 None:intergenic 40.0%
TTTAAGCGATGTGCATATGC+CGG + chr2_3:15859530-15859549 Msa0280810:CDS 40.0%
AGTTTGAATCCATCCATGTC+CGG - chr2_3:15859552-15859571 None:intergenic 40.0%
! GGAACATATTGGATTGGAGA+TGG + chr2_3:15859575-15859594 Msa0280810:CDS 40.0%
! GTAATGAAAGGTGTGACACA+CGG + chr2_3:15859743-15859762 Msa0280810:CDS 40.0%
GAAGAATATATGGCAGCATG+TGG + chr2_3:15859808-15859827 Msa0280810:CDS 40.0%
ATATATGGCAGCATGTGGTT+CGG + chr2_3:15859813-15859832 Msa0280810:CDS 40.0%
! TGGAAAGATCTGAAGCAATC+TGG + chr2_3:15859851-15859870 Msa0280810:CDS 40.0%
! AAAGATCTGAAGCAATCTGG+TGG + chr2_3:15859854-15859873 Msa0280810:CDS 40.0%
TCAATAGACCAAACAACTCG+AGG - chr2_3:15860022-15860041 None:intergenic 40.0%
GGTCATATCTCATCTAACGT+GGG - chr2_3:15860491-15860510 None:intergenic 40.0%
AGGTCATATCTCATCTAACG+TGG - chr2_3:15860492-15860511 None:intergenic 40.0%
TGGCCCATGAGATCTTATAT+AGG - chr2_3:15860607-15860626 None:intergenic 40.0%
ATGTCCACTTATCAGTGTGA+GGG + chr2_3:15860673-15860692 Msa0280810:intron 40.0%
TTGTATCTTCGAAGGTTGAG+TGG + chr2_3:15860813-15860832 Msa0280810:CDS 40.0%
AGTACGCCAGAGTCAATATT+TGG + chr2_3:15860876-15860895 Msa0280810:CDS 40.0%
!! GTTCCTCCAAATATTGACTC+TGG - chr2_3:15860885-15860904 None:intergenic 40.0%
AAACCGGTGAACTTTCTTCA+CGG + chr2_3:15861059-15861078 Msa0280810:CDS 40.0%
ATTCCGTGAAGAAAGTTCAC+CGG - chr2_3:15861065-15861084 None:intergenic 40.0%
! GCTTTGGCTCAATGTTGATA+GGG - chr2_3:15861088-15861107 None:intergenic 40.0%
! TGCTTTGGCTCAATGTTGAT+AGG - chr2_3:15861089-15861108 None:intergenic 40.0%
TTGAGCAGACTGGTGAAAAT+TGG - chr2_3:15861117-15861136 None:intergenic 40.0%
CATATACCACCAAAAGGGTA+GGG + chr2_3:15861398-15861417 Msa0280810:intron 40.0%
! GTTTGAAATGATAGGTGGCA+TGG + chr2_3:15861462-15861481 Msa0280810:intron 40.0%
GTGGCATGGATGAATACAAA+TGG + chr2_3:15861476-15861495 Msa0280810:CDS 40.0%
!! TGTTGGACTAGCAATTGCTT+TGG + chr2_3:15861577-15861596 Msa0280810:CDS 40.0%
GATTTAGCAGAAGGAGAATC+AGG - chr2_3:15861668-15861687 None:intergenic 40.0%
AAATCCTTAACATGGTTGGC+TGG + chr2_3:15861683-15861702 Msa0280810:CDS 40.0%
AAAACCAGCCAACCATGTTA+AGG - chr2_3:15861690-15861709 None:intergenic 40.0%
! TAGGATTTGTTTCGCAGTTG+CGG + chr2_3:15861930-15861949 Msa0280810:intron 40.0%
AATTGCTACAGCATCAGAGA+AGG + chr2_3:15862021-15862040 Msa0280810:CDS 40.0%
TTAGGTATAAACCAGCAGCT+AGG - chr2_3:15862089-15862108 None:intergenic 40.0%
!! CTTTGACTTTGAGGTGATCT+TGG - chr2_3:15862198-15862217 None:intergenic 40.0%
TGGTGAAGTTGAGGTTGACA+TGG - chr2_3:15858967-15858986 None:intergenic 45.0%
!! GGAGTTTGATGGTGAAGTTG+AGG - chr2_3:15858976-15858995 None:intergenic 45.0%
ATAAGGTTGCCGGAGTTTGA+TGG - chr2_3:15858987-15859006 None:intergenic 45.0%
CATCAACAACAAGTACACGG+AGG - chr2_3:15859031-15859050 None:intergenic 45.0%
CAAGGATCATGAGGCAAGTA+GGG - chr2_3:15859058-15859077 None:intergenic 45.0%
TCAAGGATCATGAGGCAAGT+AGG - chr2_3:15859059-15859078 None:intergenic 45.0%
! ATAGTGATGTCGGCTGATGA+TGG + chr2_3:15859710-15859729 Msa0280810:intron 45.0%
!! GGCTGATGATGGAAAAAACG+TGG + chr2_3:15859721-15859740 Msa0280810:CDS 45.0%
AATCAAACCTGTGCGAATCG+AGG + chr2_3:15859781-15859800 Msa0280810:CDS 45.0%
AAGGCAAGAGCTCAAAGAAG+AGG + chr2_3:15859942-15859961 Msa0280810:CDS 45.0%
CAAGAGCTCAAAGAAGAGGA+GGG + chr2_3:15859946-15859965 Msa0280810:CDS 45.0%
! CAAAGAAGCCTCGAGTTGTT+TGG + chr2_3:15860011-15860030 Msa0280810:CDS 45.0%
GTACGTTAGATGAGATACCG+TGG - chr2_3:15860155-15860174 None:intergenic 45.0%
AAACGTTGCCAGTCATCTTC+AGG + chr2_3:15860413-15860432 Msa0280810:CDS 45.0%
TATAAGATCTCATGGGCCAC+TGG + chr2_3:15860608-15860627 Msa0280810:intron 45.0%
CACACTGATAAGTGGACATC+TGG - chr2_3:15860672-15860691 None:intergenic 45.0%
GATGTCCACTTATCAGTGTG+AGG + chr2_3:15860672-15860691 Msa0280810:intron 45.0%
TGTCCACTTATCAGTGTGAG+GGG + chr2_3:15860674-15860693 Msa0280810:intron 45.0%
ACGCCAGAGTCAATATTTGG+AGG + chr2_3:15860879-15860898 Msa0280810:CDS 45.0%
CTTCAAACCCTTTCAGCTTC+TGG + chr2_3:15860924-15860943 Msa0280810:CDS 45.0%
TATGAGCAGCAGCAGTAAAC+CGG + chr2_3:15861043-15861062 Msa0280810:CDS 45.0%
CGACATGGACCGAATATTAG+TGG - chr2_3:15861336-15861355 None:intergenic 45.0%
!! CCTACCCTTTTGGTGGTATA+TGG - chr2_3:15861400-15861419 None:intergenic 45.0%
CCATATACCACCAAAAGGGT+AGG + chr2_3:15861397-15861416 Msa0280810:intron 45.0%
! GGTGGGTTTGAAATGATAGG+TGG + chr2_3:15861457-15861476 Msa0280810:intron 45.0%
GCACAAGCAGCACATAACAA+AGG - chr2_3:15861554-15861573 None:intergenic 45.0%
!!! TGTGCTGCTTGTGCTTTTGT+TGG + chr2_3:15861560-15861579 Msa0280810:CDS 45.0%
! CATGTGTGTTGATGTCGTGT+CGG + chr2_3:15861831-15861850 Msa0280810:intron 45.0%
!!! GCGGAGATTTTGTTATTGGC+AGG + chr2_3:15861949-15861968 Msa0280810:CDS 45.0%
GCAGGACTTAGTGTAGAATC+AGG + chr2_3:15861967-15861986 Msa0280810:CDS 45.0%
! AAGGTTTGTTCTCAGCTGCA+GGG + chr2_3:15862040-15862059 Msa0280810:CDS 45.0%
! ACAACAGTTTTCCTAGCTGC+TGG + chr2_3:15862075-15862094 Msa0280810:CDS 45.0%
!! TTTGTGCACGAAGTGCTGTT+AGG - chr2_3:15862107-15862126 None:intergenic 45.0%
TCGTGCACAAAGAATGTCTG+AGG + chr2_3:15862116-15862135 Msa0280810:CDS 45.0%
!! TGACTTTGAGGTGATCTTGG+TGG - chr2_3:15862195-15862214 None:intergenic 45.0%
CCTTATCTCCGACCAGTTTC+CGG + chr2_3:15859001-15859020 Msa0280810:CDS 50.0%
! TTCAAAGCCTCGATTCGCAC+AGG - chr2_3:15859791-15859810 None:intergenic 50.0%
GCAAGAGCTCAAAGAAGAGG+AGG + chr2_3:15859945-15859964 Msa0280810:CDS 50.0%
! ACGTTTTCTCTAGTGAGCCC+AGG - chr2_3:15860399-15860418 None:intergenic 50.0%
GGCTATCAAGTCACCGCTAT+TGG + chr2_3:15860629-15860648 Msa0280810:intron 50.0%
GTCCACTTATCAGTGTGAGG+GGG + chr2_3:15860675-15860694 Msa0280810:intron 50.0%
TGACTCTGGCGTACTCATGA+AGG - chr2_3:15860871-15860890 None:intergenic 50.0%
GAACTGGCCAGAAGCTGAAA+GGG - chr2_3:15860934-15860953 None:intergenic 50.0%
AGAACTGGCCAGAAGCTGAA+AGG - chr2_3:15860935-15860954 None:intergenic 50.0%
TTCAGCTTCTGGCCAGTTCT+CGG + chr2_3:15860935-15860954 Msa0280810:CDS 50.0%
! AAGTCCTGCTGCTTGAAGCT+TGG - chr2_3:15860973-15860992 None:intergenic 50.0%
CACCAGTCTGCTCAATCCTT+TGG + chr2_3:15861122-15861141 Msa0280810:CDS 50.0%
TGCCAAAGGATTGAGCAGAC+TGG - chr2_3:15861127-15861146 None:intergenic 50.0%
TCGCATATTTGCGCTGCCAA+AGG - chr2_3:15861141-15861160 None:intergenic 50.0%
! GTACATCCCTACCCTTTTGG+TGG - chr2_3:15861407-15861426 None:intergenic 50.0%
! CGTGTACATCCCTACCCTTT+TGG - chr2_3:15861410-15861429 None:intergenic 50.0%
! GAAGGTTTGTTCTCAGCTGC+AGG + chr2_3:15862039-15862058 Msa0280810:CDS 50.0%
! AGGTTTGTTCTCAGCTGCAG+GGG + chr2_3:15862041-15862060 Msa0280810:CDS 50.0%
CTGGTCGGAGATAAGGTTGC+CGG - chr2_3:15858997-15859016 None:intergenic 55.0%
CCGGAAACTGGTCGGAGATA+AGG - chr2_3:15859004-15859023 None:intergenic 55.0%
TATCTCCGACCAGTTTCCGG+CGG + chr2_3:15859004-15859023 Msa0280810:CDS 55.0%
CGATGTGCATATGCCGGACA+TGG + chr2_3:15859536-15859555 Msa0280810:CDS 55.0%
GTGCATATGCCGGACATGGA+TGG + chr2_3:15859540-15859559 Msa0280810:CDS 55.0%
! AGCGGTGACTTGATAGCCAG+TGG - chr2_3:15860627-15860646 None:intergenic 55.0%
! ATCTGGAGTGTGACTCGAGC+GGG - chr2_3:15860655-15860674 None:intergenic 55.0%
! CATCTGGAGTGTGACTCGAG+CGG - chr2_3:15860656-15860675 None:intergenic 55.0%
CACCCCCTCACACTGATAAG+TGG - chr2_3:15860680-15860699 None:intergenic 55.0%
CTAGACTTTGCGCCGAGAAC+TGG - chr2_3:15860950-15860969 None:intergenic 55.0%
CTAGCCAAGCTTCAAGCAGC+AGG + chr2_3:15860966-15860985 Msa0280810:CDS 55.0%
ATCTCCGACCAGTTTCCGGC+GGG + chr2_3:15859005-15859024 Msa0280810:CDS 60.0%
TGACTCGAGCGGGCCAATAG+CGG - chr2_3:15860645-15860664 None:intergenic 60.0%
ACAAGTACACGGAGGCCCGC+CGG - chr2_3:15859023-15859042 None:intergenic 65.0%
GAGGCCCGCCGGAAACTGGT+CGG - chr2_3:15859012-15859031 None:intergenic 70.0%
CACGGAGGCCCGCCGGAAAC+TGG - chr2_3:15859016-15859035 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 15858731 15862329 15858731 ID=Msa0280810;Name=Msa0280810
chr2_3 mRNA 15858731 15862329 15858731 ID=Msa0280810-mRNA-1;Parent=Msa0280810;Name=Msa0280810-mRNA-1;_AED=0.21;_eAED=0.21;_QI=205|0.5|0.42|1|1|1|7|0|593
chr2_3 exon 15858731 15859107 15858731 ID=Msa0280810-mRNA-1:exon:6585;Parent=Msa0280810-mRNA-1
chr2_3 exon 15859460 15859612 15859460 ID=Msa0280810-mRNA-1:exon:6586;Parent=Msa0280810-mRNA-1
chr2_3 exon 15859714 15860088 15859714 ID=Msa0280810-mRNA-1:exon:6587;Parent=Msa0280810-mRNA-1
chr2_3 exon 15860358 15860434 15860358 ID=Msa0280810-mRNA-1:exon:6588;Parent=Msa0280810-mRNA-1
chr2_3 exon 15860804 15861160 15860804 ID=Msa0280810-mRNA-1:exon:6589;Parent=Msa0280810-mRNA-1
chr2_3 exon 15861476 15861726 15861476 ID=Msa0280810-mRNA-1:exon:6590;Parent=Msa0280810-mRNA-1
chr2_3 exon 15861933 15862329 15861933 ID=Msa0280810-mRNA-1:exon:6591;Parent=Msa0280810-mRNA-1
chr2_3 five_prime_UTR 15858731 15858935 15858731 ID=Msa0280810-mRNA-1:five_prime_utr;Parent=Msa0280810-mRNA-1
chr2_3 CDS 15858936 15859107 15858936 ID=Msa0280810-mRNA-1:cds;Parent=Msa0280810-mRNA-1
chr2_3 CDS 15859460 15859612 15859460 ID=Msa0280810-mRNA-1:cds;Parent=Msa0280810-mRNA-1
chr2_3 CDS 15859714 15860088 15859714 ID=Msa0280810-mRNA-1:cds;Parent=Msa0280810-mRNA-1
chr2_3 CDS 15860358 15860434 15860358 ID=Msa0280810-mRNA-1:cds;Parent=Msa0280810-mRNA-1
chr2_3 CDS 15860804 15861160 15860804 ID=Msa0280810-mRNA-1:cds;Parent=Msa0280810-mRNA-1
chr2_3 CDS 15861476 15861726 15861476 ID=Msa0280810-mRNA-1:cds;Parent=Msa0280810-mRNA-1
chr2_3 CDS 15861933 15862329 15861933 ID=Msa0280810-mRNA-1:cds;Parent=Msa0280810-mRNA-1
Gene Sequence

>Msa0280810

ATGAAACTCAACAACAACAACAAAATTTCCATGTCAACCTCAACTTCACCATCAAACTCCGGCAACCTTATCTCCGACCAGTTTCCGGCGGGCCTCCGTGTACTTGTTGTTGATGATGACCCTACTTGCCTCATGATCCTTGAAAGAATGCTCCGTTTTTGCCTTTATGAAGTTACCAAATGTAAGAGAGCTGAGGTTGCATTATCACTTCTTAGAGAGAATAAAAATGGGTTCGACATTGTTTTAAGCGATGTGCATATGCCGGACATGGATGGATTCAAACTTTTGGAACATATTGGATTGGAGATGGATCTTCCTGTTATTATGATGTCGGCTGATGATGGAAAAAACGTGGTAATGAAAGGTGTGACACACGGTGCTTGTGATTATCTAATCAAACCTGTGCGAATCGAGGCTTTGAAGAATATATGGCAGCATGTGGTTCGGAAGAGAAAGAATAACTGGAAAGATCTGAAGCAATCTGGTGGATTAGATGAAAGAGATCGAGAAAAAATTGTGTCCACTGATGCTAATTACTCATCTTCAACGAATGAAGGCAAGAGCTCAAAGAAGAGGAGGGATGAAGAAGATGAAGCAGATGATAGGGATGATTGTTCGACGTCAAAGAAGCCTCGAGTTGTTTGGTCTATTGAGCTTCATCAACAGTTTATGGCTGTTGTGAATCAATTGGGATTAGACAAGGCTGTTCCTAAAAAGATTATGGAAATGATGAATGTTCCTGGGCTCACTAGAGAAAACGTTGCCAGTCATCTTCAGAAATACCGTTTGTATCTTCGAAGGTTGAGTGGAGTTTCCCAGCAGCAAAGTAACTTGAATAACTCCTTCATGAGTACGCCAGAGTCAATATTTGGAGGAACCTCCGTAAATGAAATTGATCTTCAAACCCTTTCAGCTTCTGGCCAGTTCTCGGCGCAAAGTCTAGCCAAGCTTCAAGCAGCAGGACTTTACAGTCCTGAGAACTCAAAACTAAGATTTGGAGATGGGCAACTGCAACATATGAGCAGCAGCAGTAAACCGGTGAACTTTCTTCACGGAATCCCTATCAACATTGAGCCAAAGCAGTTTGCCAATTTTCACCAGTCTGCTCAATCCTTTGGCAGCGCAAATATGCGAGTGGCATGGATGAATACAAATGGAAAAGAAAAGGGAACAAAATCTGAATGTGTATACAATAGTAATGGGAAAGTGCCTTTGTTATGTGCTGCTTGTGCTTTTGTTGGACTAGCAATTGCTTTGGTTATGGAGCATACTTACATGTTGATAGCAGTGAGTGAATCATCACCTTCATTAATTAATTGGGATCCTGATTCTCCTTCTGCTAAATCCTTAACATGGTTGGCTGGTTTTTTCTTCATTACAACTTGGATTTGTTTCGCAGTTGCGGAGATTTTGTTATTGGCAGGACTTAGTGTAGAATCAGGTCATTTAAAAAATTGGACTAAGCCAAGAACAAATTGCTACAGCATCAGAGAAGGTTTGTTCTCAGCTGCAGGGGTGTTTGCTTTAACAACAGTTTTCCTAGCTGCTGGTTTATACCTAACAGCACTTCGTGCACAAAGAATGTCTGAGGAGCTTGCAATTGTTAGAAGAGAAGTTCTTGAAGCTTCTAACTTTTATGCTTCTCCACCAAGATCACCTCAAAGTCAAAGACATATTGCAACTGTTGCAAGAGAGAACCCTACAACAATTGAGAGTCAAAATGATAATTTATTATTATCTGTGTTTCCAACCCCTTTTAATAAAAGTTATACCTTTGTATAA
Protein sequence

>Msa0280810

MKLNNNNKISMSTSTSPSNSGNLISDQFPAGLRVLVVDDDPTCLMILERMLRFCLYEVTKCKRAEVALSLLRENKNGFDIVLSDVHMPDMDGFKLLEHIGLEMDLPVIMMSADDGKNVVMKGVTHGACDYLIKPVRIEALKNIWQHVVRKRKNNWKDLKQSGGLDERDREKIVSTDANYSSSTNEGKSSKKRRDEEDEADDRDDCSTSKKPRVVWSIELHQQFMAVVNQLGLDKAVPKKIMEMMNVPGLTRENVASHLQKYRLYLRRLSGVSQQQSNLNNSFMSTPESIFGGTSVNEIDLQTLSASGQFSAQSLAKLQAAGLYSPENSKLRFGDGQLQHMSSSSKPVNFLHGIPINIEPKQFANFHQSAQSFGSANMRVAWMNTNGKEKGTKSECVYNSNGKVPLLCAACAFVGLAIALVMEHTYMLIAVSESSPSLINWDPDSPSAKSLTWLAGFFFITTWICFAVAEILLLAGLSVESGHLKNWTKPRTNCYSIREGLFSAAGVFALTTVFLAAGLYLTALRAQRMSEELAIVRREVLEASNFYASPPRSPQSQRHIATVARENPTTIESQNDNLLLSVFPTPFNKSYTFV*