AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0282040


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0282040 MtrunA17_Chr2g0296861 91.139 158 14 0 1 158 1 158 2.08e-93 267
Msa0282040 MtrunA17_Chr3g0107381 46.203 158 85 0 1 158 1 158 2.13e-46 150
Msa0282040 MtrunA17_Chr3g0107461 49.351 154 77 1 1 153 1 154 4.64e-46 147
Msa0282040 MtrunA17_Chr3g0110131 44.156 154 86 0 1 154 1 154 4.13e-38 130
Msa0282040 MtrunA17_Chr4g0014321 37.662 154 95 1 1 153 1 154 6.75e-35 121
Msa0282040 MtrunA17_Chr3g0105791 47.541 122 63 1 30 150 2 123 1.07e-33 115
Msa0282040 MtrunA17_Chr4g0031421 46.400 125 66 1 30 153 2 126 3.21e-33 114
Msa0282040 MtrunA17_Chr2g0283941 38.750 160 93 2 1 157 1 158 5.38e-33 117
Msa0282040 MtrunA17_Chr3g0107331 39.597 149 90 0 4 152 3 151 6.29e-33 114
Msa0282040 MtrunA17_Chr4g0013041 35.849 159 99 2 1 158 1 157 5.87e-32 113
Msa0282040 MtrunA17_Chr5g0432691 33.333 153 102 0 1 153 1 153 1.15e-30 108
Msa0282040 MtrunA17_Chr4g0014031 37.179 156 97 1 1 155 1 156 2.11e-29 107
Msa0282040 MtrunA17_Chr8g0351751 37.975 158 95 2 1 155 1 158 2.40e-29 107
Msa0282040 MtrunA17_Chr3g0107361 36.486 148 94 0 6 153 5 152 3.57e-29 104
Msa0282040 MtrunA17_Chr3g0106421 42.308 130 74 1 30 158 2 131 9.96e-29 105
Msa0282040 MtrunA17_Chr1g0197461 33.333 153 101 1 6 158 5 156 1.41e-28 103
Msa0282040 MtrunA17_Chr3g0107351 35.135 148 96 0 6 153 5 152 2.94e-28 102
Msa0282040 MtrunA17_Chr5g0420351 34.194 155 101 1 1 155 1 154 3.88e-28 102
Msa0282040 MtrunA17_Chr4g0014121 36.943 157 98 1 1 156 29 185 3.34e-27 100
Msa0282040 MtrunA17_Chr1g0188681 33.987 153 100 1 1 153 1 152 4.30e-27 99.4
Msa0282040 MtrunA17_Chr3g0107341 35.417 144 93 0 6 149 5 148 2.21e-26 97.8
Msa0282040 MtrunA17_Chr1g0197261 30.968 155 106 1 1 155 1 154 9.26e-24 90.9
Msa0282040 MtrunA17_Chr2g0296831 36.752 117 74 0 1 117 1 117 1.84e-23 89.0
Msa0282040 MtrunA17_Chr4g0014131 35.032 157 101 1 1 156 1 157 8.50e-23 90.1
Msa0282040 MtrunA17_Chr3g0106501 38.583 127 77 1 30 155 2 128 4.20e-22 86.7
Msa0282040 MtrunA17_Chr4g0012761 35.897 156 97 3 1 155 1 154 4.58e-22 86.7
Msa0282040 MtrunA17_Chr1g0197321 31.210 157 104 3 1 155 1 155 9.25e-22 85.9
Msa0282040 MtrunA17_Chr7g0218151 32.903 155 103 1 1 155 1 154 1.26e-21 85.5
Msa0282040 MtrunA17_Chr1g0193311 34.194 155 101 1 1 155 1 154 2.27e-21 84.7
Msa0282040 MtrunA17_Chr1g0188661 30.323 155 107 1 1 155 1 154 4.85e-20 81.3
Msa0282040 MtrunA17_Chr1g0188651 36.129 155 92 4 1 153 1 150 4.20e-18 76.3
Msa0282040 MtrunA17_Chr4g0008701 28.814 118 80 1 11 128 14 127 4.10e-11 60.1
Msa0282040 MtrunA17_Chr7g0267601 27.273 121 78 2 11 128 14 127 5.27e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0282040 AT5G48670.1 41.667 156 87 2 1 154 1 154 7.08e-32 116
Msa0282040 AT3G05860.2 36.913 149 94 0 1 149 1 149 2.57e-29 107
Msa0282040 AT1G65300.1 37.086 151 95 0 4 154 3 153 3.13e-29 108
Msa0282040 AT1G65330.1 39.333 150 89 2 6 154 5 153 3.55e-29 108
Msa0282040 AT3G05860.3 39.103 156 81 3 1 149 1 149 1.37e-28 106
Msa0282040 AT3G05860.1 39.103 156 81 3 1 149 1 149 1.52e-28 106
Msa0282040 AT1G31630.1 33.333 153 91 3 6 153 5 151 7.02e-24 95.5
Msa0282040 AT1G31640.1 35.915 142 90 1 11 152 10 150 2.05e-23 95.5
Msa0282040 AT5G27810.1 43.182 88 50 0 30 117 2 89 7.67e-23 87.8
Msa0282040 AT5G26650.1 32.237 152 103 0 4 155 2 153 1.74e-22 92.0
Msa0282040 AT5G27960.1 31.579 152 104 0 4 155 2 153 1.39e-21 89.0
Msa0282040 AT1G22590.2 36.111 108 69 0 11 118 11 118 5.23e-21 84.3
Msa0282040 AT2G28700.1 32.680 153 95 3 1 149 1 149 6.04e-20 84.7
Msa0282040 AT2G40210.1 32.479 117 79 0 1 117 1 117 9.62e-15 70.5
Msa0282040 AT5G26580.1 28.571 154 96 3 1 154 1 140 2.97e-14 69.3
Msa0282040 AT5G26630.1 26.623 154 113 0 1 154 1 154 3.35e-14 67.8
Msa0282040 AT5G06500.1 28.182 110 75 1 1 110 1 106 4.39e-13 65.1

Find 26 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGATCTATCATTGCTTAT+TGG 0.201999 2_3:-17746885 Msa0282040:CDS
CTTCTCTTTCGATTTCATTA+TGG 0.221424 2_3:+17747013 None:intergenic
ATTCATACTGGTGAATTTGA+TGG 0.332075 2_3:-17746930 Msa0282040:CDS
AGATGGTGTTGGACAAGTTT+AGG 0.350542 2_3:-17747097 Msa0282040:CDS
CTTGGTGAATTCATTCATAC+TGG 0.375906 2_3:-17746942 Msa0282040:CDS
GTTTAGGAGCTTTCCTGAAT+CGG 0.417309 2_3:-17747081 Msa0282040:CDS
TAAACTTGTCCAACACCATC+TGG 0.418271 2_3:+17747099 None:intergenic
AGTGAAATCACCACACTTTG+TGG 0.451943 2_3:-17747194 Msa0282040:CDS
GGAAGACAACATTCAACAAA+AGG 0.464060 2_3:-17747625 Msa0282040:CDS
ATCTATGATGAAAACAATGA+TGG 0.489972 2_3:-17747152 Msa0282040:CDS
AGAGAAGTTGAAGAAACAAA+AGG 0.500916 2_3:-17746997 Msa0282040:CDS
CCTGAATCGGAACAAAGAAA+AGG 0.516491 2_3:-17747068 Msa0282040:CDS
GGAACAAAGAAAAGGAAAGC+TGG 0.520124 2_3:-17747060 Msa0282040:CDS
AGTTGAAGAAACAAAAGGAC+AGG 0.532305 2_3:-17746992 Msa0282040:CDS
AAAAGGAAAGCTGGATCATG+AGG 0.532487 2_3:-17747051 Msa0282040:CDS
AAGAAAATGAATGATCTACT+TGG 0.551967 2_3:-17746960 Msa0282040:CDS
ACGGTTTATCCATCAAAAGA+AGG 0.566324 2_3:-17747125 Msa0282040:CDS
ACATAACTGATGACTTGAAG+AGG 0.573872 2_3:-17747646 Msa0282040:CDS
AGAAGGAGTCCAGATGGTGT+TGG 0.590630 2_3:-17747108 Msa0282040:CDS
ACCGAAAAGAAGTCGATGAG+AGG 0.615793 2_3:-17746857 Msa0282040:CDS
ATCAAAAGAAGGAGTCCAGA+TGG 0.615817 2_3:-17747114 Msa0282040:CDS
TGCATCAATTCCACAAAGTG+TGG 0.616904 2_3:+17747184 None:intergenic
TGAAAACAATGATGGAGCAA+CGG 0.627126 2_3:-17747144 Msa0282040:CDS
ACATTCAACAAAAGGAAGCA+TGG 0.630577 2_3:-17747617 Msa0282040:intron
AAAGGAAAGCTGGATCATGA+GGG 0.661580 2_3:-17747050 Msa0282040:CDS
CTTTGTGGAATTGATGCATG+TGG 0.684212 2_3:-17747179 Msa0282040:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGTGTTCTTTATAGAAATAA+AGG + chr2_3:17746936-17746955 None:intergenic 20.0%
!!! TTTCATATTTTTAGTATCCA+TGG + chr2_3:17747026-17747045 None:intergenic 20.0%
!! TCATATTGATAAATTCCTAA+TGG + chr2_3:17747062-17747081 None:intergenic 20.0%
!! TCATAACTGATATGTTATTA+TGG + chr2_3:17747162-17747181 None:intergenic 20.0%
!! GAAAATAACGATAATTTCAA+AGG + chr2_3:17747185-17747204 None:intergenic 20.0%
!! GAAAAAATTTATACTATAGC+TGG + chr2_3:17747227-17747246 None:intergenic 20.0%
!! AGTGTTCTTTATAGAAATAA+AGG + chr2_3:17746936-17746955 None:intergenic 20.0%
!!! TTTCATATTTTTAGTATCCA+TGG + chr2_3:17747026-17747045 None:intergenic 20.0%
!! TCATATTGATAAATTCCTAA+TGG + chr2_3:17747062-17747081 None:intergenic 20.0%
!! TCATAACTGATATGTTATTA+TGG + chr2_3:17747162-17747181 None:intergenic 20.0%
!! GAAAATAACGATAATTTCAA+AGG + chr2_3:17747185-17747204 None:intergenic 20.0%
!! GAAAAAATTTATACTATAGC+TGG + chr2_3:17747227-17747246 None:intergenic 20.0%
!!! GTTTTTTTTTATCACTACCA+TGG - chr2_3:17747006-17747025 Msa0282040:CDS 25.0%
! ATCTATGATGAAAACAATGA+TGG - chr2_3:17747358-17747377 Msa0282040:intron 25.0%
!! ATCATTCATTTTCTTCTTCT+TGG + chr2_3:17747545-17747564 None:intergenic 25.0%
! AAGAAAATGAATGATCTACT+TGG - chr2_3:17747550-17747569 Msa0282040:intron 25.0%
!!! GTTTTTTTTTATCACTACCA+TGG - chr2_3:17747006-17747025 Msa0282040:CDS 25.0%
! ATCTATGATGAAAACAATGA+TGG - chr2_3:17747358-17747377 Msa0282040:intron 25.0%
!! ATCATTCATTTTCTTCTTCT+TGG + chr2_3:17747545-17747564 None:intergenic 25.0%
! AAGAAAATGAATGATCTACT+TGG - chr2_3:17747550-17747569 Msa0282040:intron 25.0%
!! GTTTTGTTGTGTTTTTCAAC+AGG - chr2_3:17747277-17747296 Msa0282040:intron 30.0%
CTTCTCTTTCGATTTCATTA+TGG + chr2_3:17747500-17747519 None:intergenic 30.0%
AGAGAAGTTGAAGAAACAAA+AGG - chr2_3:17747513-17747532 Msa0282040:intron 30.0%
ATTCATACTGGTGAATTTGA+TGG - chr2_3:17747580-17747599 Msa0282040:intron 30.0%
GAAGATCTATCATTGCTTAT+TGG - chr2_3:17747625-17747644 Msa0282040:CDS 30.0%
!! GTTTTGTTGTGTTTTTCAAC+AGG - chr2_3:17747277-17747296 Msa0282040:intron 30.0%
CTTCTCTTTCGATTTCATTA+TGG + chr2_3:17747500-17747519 None:intergenic 30.0%
AGAGAAGTTGAAGAAACAAA+AGG - chr2_3:17747513-17747532 Msa0282040:intron 30.0%
ATTCATACTGGTGAATTTGA+TGG - chr2_3:17747580-17747599 Msa0282040:intron 30.0%
GAAGATCTATCATTGCTTAT+TGG - chr2_3:17747625-17747644 Msa0282040:CDS 30.0%
ACATAACTGATGACTTGAAG+AGG - chr2_3:17746864-17746883 Msa0282040:CDS 35.0%
GGAAGACAACATTCAACAAA+AGG - chr2_3:17746885-17746904 Msa0282040:CDS 35.0%
ACATTCAACAAAAGGAAGCA+TGG - chr2_3:17746893-17746912 Msa0282040:CDS 35.0%
AAGTTGACTGCATAACCATT+AGG - chr2_3:17747044-17747063 Msa0282040:CDS 35.0%
! TGAAAACAATGATGGAGCAA+CGG - chr2_3:17747366-17747385 Msa0282040:intron 35.0%
ACGGTTTATCCATCAAAAGA+AGG - chr2_3:17747385-17747404 Msa0282040:intron 35.0%
AGTTGAAGAAACAAAAGGAC+AGG - chr2_3:17747518-17747537 Msa0282040:intron 35.0%
CTTGGTGAATTCATTCATAC+TGG - chr2_3:17747568-17747587 Msa0282040:intron 35.0%
ACATAACTGATGACTTGAAG+AGG - chr2_3:17746864-17746883 Msa0282040:CDS 35.0%
GGAAGACAACATTCAACAAA+AGG - chr2_3:17746885-17746904 Msa0282040:CDS 35.0%
ACATTCAACAAAAGGAAGCA+TGG - chr2_3:17746893-17746912 Msa0282040:CDS 35.0%
AAGTTGACTGCATAACCATT+AGG - chr2_3:17747044-17747063 Msa0282040:CDS 35.0%
! TGAAAACAATGATGGAGCAA+CGG - chr2_3:17747366-17747385 Msa0282040:intron 35.0%
ACGGTTTATCCATCAAAAGA+AGG - chr2_3:17747385-17747404 Msa0282040:intron 35.0%
AGTTGAAGAAACAAAAGGAC+AGG - chr2_3:17747518-17747537 Msa0282040:intron 35.0%
CTTGGTGAATTCATTCATAC+TGG - chr2_3:17747568-17747587 Msa0282040:intron 35.0%
AGTGAAATCACCACACTTTG+TGG - chr2_3:17747316-17747335 Msa0282040:intron 40.0%
TGCATCAATTCCACAAAGTG+TGG + chr2_3:17747329-17747348 None:intergenic 40.0%
CTTTGTGGAATTGATGCATG+TGG - chr2_3:17747331-17747350 Msa0282040:intron 40.0%
! ATCTGGACTCCTTCTTTTGA+TGG + chr2_3:17747397-17747416 None:intergenic 40.0%
ATCAAAAGAAGGAGTCCAGA+TGG - chr2_3:17747396-17747415 Msa0282040:intron 40.0%
TAAACTTGTCCAACACCATC+TGG + chr2_3:17747414-17747433 None:intergenic 40.0%
! AGATGGTGTTGGACAAGTTT+AGG - chr2_3:17747413-17747432 Msa0282040:intron 40.0%
GTTTAGGAGCTTTCCTGAAT+CGG - chr2_3:17747429-17747448 Msa0282040:intron 40.0%
! CCTTTTCTTTGTTCCGATTC+AGG + chr2_3:17747445-17747464 None:intergenic 40.0%
CCTGAATCGGAACAAAGAAA+AGG - chr2_3:17747442-17747461 Msa0282040:intron 40.0%
GGAACAAAGAAAAGGAAAGC+TGG - chr2_3:17747450-17747469 Msa0282040:intron 40.0%
AAAAGGAAAGCTGGATCATG+AGG - chr2_3:17747459-17747478 Msa0282040:intron 40.0%
AAAGGAAAGCTGGATCATGA+GGG - chr2_3:17747460-17747479 Msa0282040:intron 40.0%
!!! TCCTCTCATCGACTTCTTTT+CGG + chr2_3:17747657-17747676 None:intergenic 40.0%
AGTGAAATCACCACACTTTG+TGG - chr2_3:17747316-17747335 Msa0282040:intron 40.0%
TGCATCAATTCCACAAAGTG+TGG + chr2_3:17747329-17747348 None:intergenic 40.0%
CTTTGTGGAATTGATGCATG+TGG - chr2_3:17747331-17747350 Msa0282040:intron 40.0%
! ATCTGGACTCCTTCTTTTGA+TGG + chr2_3:17747397-17747416 None:intergenic 40.0%
ATCAAAAGAAGGAGTCCAGA+TGG - chr2_3:17747396-17747415 Msa0282040:intron 40.0%
TAAACTTGTCCAACACCATC+TGG + chr2_3:17747414-17747433 None:intergenic 40.0%
! AGATGGTGTTGGACAAGTTT+AGG - chr2_3:17747413-17747432 Msa0282040:intron 40.0%
GTTTAGGAGCTTTCCTGAAT+CGG - chr2_3:17747429-17747448 Msa0282040:intron 40.0%
! CCTTTTCTTTGTTCCGATTC+AGG + chr2_3:17747445-17747464 None:intergenic 40.0%
CCTGAATCGGAACAAAGAAA+AGG - chr2_3:17747442-17747461 Msa0282040:intron 40.0%
GGAACAAAGAAAAGGAAAGC+TGG - chr2_3:17747450-17747469 Msa0282040:intron 40.0%
AAAAGGAAAGCTGGATCATG+AGG - chr2_3:17747459-17747478 Msa0282040:intron 40.0%
AAAGGAAAGCTGGATCATGA+GGG - chr2_3:17747460-17747479 Msa0282040:intron 40.0%
!!! TCCTCTCATCGACTTCTTTT+CGG + chr2_3:17747657-17747676 None:intergenic 40.0%
ATTTCCCTCAACTCGCTCAT+GGG - chr2_3:17747255-17747274 Msa0282040:intron 45.0%
! AAAACCCATGAGCGAGTTGA+GGG + chr2_3:17747262-17747281 None:intergenic 45.0%
ACCGAAAAGAAGTCGATGAG+AGG - chr2_3:17747653-17747672 Msa0282040:CDS 45.0%
ATTTCCCTCAACTCGCTCAT+GGG - chr2_3:17747255-17747274 Msa0282040:intron 45.0%
! AAAACCCATGAGCGAGTTGA+GGG + chr2_3:17747262-17747281 None:intergenic 45.0%
ACCGAAAAGAAGTCGATGAG+AGG - chr2_3:17747653-17747672 Msa0282040:CDS 45.0%
CATTTCCCTCAACTCGCTCA+TGG - chr2_3:17747254-17747273 Msa0282040:intron 50.0%
CAAAACCCATGAGCGAGTTG+AGG + chr2_3:17747263-17747282 None:intergenic 50.0%
AGAAGGAGTCCAGATGGTGT+TGG - chr2_3:17747402-17747421 Msa0282040:intron 50.0%
CATTTCCCTCAACTCGCTCA+TGG - chr2_3:17747254-17747273 Msa0282040:intron 50.0%
CAAAACCCATGAGCGAGTTG+AGG + chr2_3:17747263-17747282 None:intergenic 50.0%
AGAAGGAGTCCAGATGGTGT+TGG - chr2_3:17747402-17747421 Msa0282040:intron 50.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 17746836 17747696 17746836 ID=Msa0282040;Name=Msa0282040
chr2_3 mRNA 17746836 17747696 17746836 ID=Msa0282040-mRNA-1;Parent=Msa0282040;Name=Msa0282040-mRNA-1;_AED=0.29;_eAED=0.29;_QI=0|0|0|1|1|1|2|0|158
chr2_3 exon 17747618 17747696 17747618 ID=Msa0282040-mRNA-1:exon:7308;Parent=Msa0282040-mRNA-1
chr2_3 exon 17746836 17747233 17746836 ID=Msa0282040-mRNA-1:exon:7307;Parent=Msa0282040-mRNA-1
chr2_3 CDS 17747618 17747696 17747618 ID=Msa0282040-mRNA-1:cds;Parent=Msa0282040-mRNA-1
chr2_3 CDS 17746836 17747233 17746836 ID=Msa0282040-mRNA-1:cds;Parent=Msa0282040-mRNA-1
Gene Sequence

>Msa0282040

ATGGCTGATAAAAATATGAGAGTTGACTACATAACTGATGACTTGAAGAGGAAGACAACATTCAACAAAAGGAAGCATGGTATAACTAAGAAGATTAGTGAAATCACCACACTTTGTGGAATTGATGCATGTGGTATAATCTATGATGAAAACAATGATGGAGCAACGGTTTATCCATCAAAAGAAGGAGTCCAGATGGTGTTGGACAAGTTTAGGAGCTTTCCTGAATCGGAACAAAGAAAAGGAAAGCTGGATCATGAGGGCTTTTTGAGACAATCCATAATGAAATCGAAAGAGAAGTTGAAGAAACAAAAGGACAGGTCCAAGAAGAAGAAAATGAATGATCTACTTGGTGAATTCATTCATACTGGTGAATTTGATGGAAATGTGAGCGAGAATGATCTCGAAGATCTATCATTGCTTATTGGTGATTACCGAAAAGAAGTCGATGAGAGGATCGAATCAAGTCAAACCTGA

Protein sequence

>Msa0282040

MADKNMRVDYITDDLKRKTTFNKRKHGITKKISEITTLCGIDACGIIYDENNDGATVYPSKEGVQMVLDKFRSFPESEQRKGKLDHEGFLRQSIMKSKEKLKKQKDRSKKKKMNDLLGEFIHTGEFDGNVSENDLEDLSLLIGDYRKEVDERIESSQT*