AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0282120


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0282120 MtrunA17_Chr2g0296861 91.139 158 14 0 1 158 1 158 2.08e-93 267
Msa0282120 MtrunA17_Chr3g0107381 46.203 158 85 0 1 158 1 158 2.13e-46 150
Msa0282120 MtrunA17_Chr3g0107461 49.351 154 77 1 1 153 1 154 4.64e-46 147
Msa0282120 MtrunA17_Chr3g0110131 44.156 154 86 0 1 154 1 154 4.13e-38 130
Msa0282120 MtrunA17_Chr4g0014321 37.662 154 95 1 1 153 1 154 6.75e-35 121
Msa0282120 MtrunA17_Chr3g0105791 47.541 122 63 1 30 150 2 123 1.07e-33 115
Msa0282120 MtrunA17_Chr4g0031421 46.400 125 66 1 30 153 2 126 3.21e-33 114
Msa0282120 MtrunA17_Chr2g0283941 38.750 160 93 2 1 157 1 158 5.38e-33 117
Msa0282120 MtrunA17_Chr3g0107331 39.597 149 90 0 4 152 3 151 6.29e-33 114
Msa0282120 MtrunA17_Chr4g0013041 35.849 159 99 2 1 158 1 157 5.87e-32 113
Msa0282120 MtrunA17_Chr5g0432691 33.333 153 102 0 1 153 1 153 1.15e-30 108
Msa0282120 MtrunA17_Chr4g0014031 37.179 156 97 1 1 155 1 156 2.11e-29 107
Msa0282120 MtrunA17_Chr8g0351751 37.975 158 95 2 1 155 1 158 2.40e-29 107
Msa0282120 MtrunA17_Chr3g0107361 36.486 148 94 0 6 153 5 152 3.57e-29 104
Msa0282120 MtrunA17_Chr3g0106421 42.308 130 74 1 30 158 2 131 9.96e-29 105
Msa0282120 MtrunA17_Chr1g0197461 33.333 153 101 1 6 158 5 156 1.41e-28 103
Msa0282120 MtrunA17_Chr3g0107351 35.135 148 96 0 6 153 5 152 2.94e-28 102
Msa0282120 MtrunA17_Chr5g0420351 34.194 155 101 1 1 155 1 154 3.88e-28 102
Msa0282120 MtrunA17_Chr4g0014121 36.943 157 98 1 1 156 29 185 3.34e-27 100
Msa0282120 MtrunA17_Chr1g0188681 33.987 153 100 1 1 153 1 152 4.30e-27 99.4
Msa0282120 MtrunA17_Chr3g0107341 35.417 144 93 0 6 149 5 148 2.21e-26 97.8
Msa0282120 MtrunA17_Chr1g0197261 30.968 155 106 1 1 155 1 154 9.26e-24 90.9
Msa0282120 MtrunA17_Chr2g0296831 36.752 117 74 0 1 117 1 117 1.84e-23 89.0
Msa0282120 MtrunA17_Chr4g0014131 35.032 157 101 1 1 156 1 157 8.50e-23 90.1
Msa0282120 MtrunA17_Chr3g0106501 38.583 127 77 1 30 155 2 128 4.20e-22 86.7
Msa0282120 MtrunA17_Chr4g0012761 35.897 156 97 3 1 155 1 154 4.58e-22 86.7
Msa0282120 MtrunA17_Chr1g0197321 31.210 157 104 3 1 155 1 155 9.25e-22 85.9
Msa0282120 MtrunA17_Chr7g0218151 32.903 155 103 1 1 155 1 154 1.26e-21 85.5
Msa0282120 MtrunA17_Chr1g0193311 34.194 155 101 1 1 155 1 154 2.27e-21 84.7
Msa0282120 MtrunA17_Chr1g0188661 30.323 155 107 1 1 155 1 154 4.85e-20 81.3
Msa0282120 MtrunA17_Chr1g0188651 36.129 155 92 4 1 153 1 150 4.20e-18 76.3
Msa0282120 MtrunA17_Chr4g0008701 28.814 118 80 1 11 128 14 127 4.10e-11 60.1
Msa0282120 MtrunA17_Chr7g0267601 27.273 121 78 2 11 128 14 127 5.27e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0282120 AT5G48670.1 41.667 156 87 2 1 154 1 154 7.08e-32 116
Msa0282120 AT3G05860.2 36.913 149 94 0 1 149 1 149 2.57e-29 107
Msa0282120 AT1G65300.1 37.086 151 95 0 4 154 3 153 3.13e-29 108
Msa0282120 AT1G65330.1 39.333 150 89 2 6 154 5 153 3.55e-29 108
Msa0282120 AT3G05860.3 39.103 156 81 3 1 149 1 149 1.37e-28 106
Msa0282120 AT3G05860.1 39.103 156 81 3 1 149 1 149 1.52e-28 106
Msa0282120 AT1G31630.1 33.333 153 91 3 6 153 5 151 7.02e-24 95.5
Msa0282120 AT1G31640.1 35.915 142 90 1 11 152 10 150 2.05e-23 95.5
Msa0282120 AT5G27810.1 43.182 88 50 0 30 117 2 89 7.67e-23 87.8
Msa0282120 AT5G26650.1 32.237 152 103 0 4 155 2 153 1.74e-22 92.0
Msa0282120 AT5G27960.1 31.579 152 104 0 4 155 2 153 1.39e-21 89.0
Msa0282120 AT1G22590.2 36.111 108 69 0 11 118 11 118 5.23e-21 84.3
Msa0282120 AT2G28700.1 32.680 153 95 3 1 149 1 149 6.04e-20 84.7
Msa0282120 AT2G40210.1 32.479 117 79 0 1 117 1 117 9.62e-15 70.5
Msa0282120 AT5G26580.1 28.571 154 96 3 1 154 1 140 2.97e-14 69.3
Msa0282120 AT5G26630.1 26.623 154 113 0 1 154 1 154 3.35e-14 67.8
Msa0282120 AT5G06500.1 28.182 110 75 1 1 110 1 106 4.39e-13 65.1

Find 26 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGATCTATCATTGCTTAT+TGG 0.201999 2_3:+17885128 Msa0282120:CDS
CTTCTCTTTCGATTTCATTA+TGG 0.221424 2_3:-17885000 None:intergenic
ATTCATACTGGTGAATTTGA+TGG 0.332075 2_3:+17885083 Msa0282120:CDS
AGATGGTGTTGGACAAGTTT+AGG 0.350542 2_3:+17884916 Msa0282120:CDS
CTTGGTGAATTCATTCATAC+TGG 0.375906 2_3:+17885071 Msa0282120:CDS
GTTTAGGAGCTTTCCTGAAT+CGG 0.417309 2_3:+17884932 Msa0282120:CDS
TAAACTTGTCCAACACCATC+TGG 0.418271 2_3:-17884914 None:intergenic
AGTGAAATCACCACACTTTG+TGG 0.451943 2_3:+17884819 Msa0282120:CDS
GGAAGACAACATTCAACAAA+AGG 0.464060 2_3:+17884388 Msa0282120:CDS
ATCTATGATGAAAACAATGA+TGG 0.489972 2_3:+17884861 Msa0282120:CDS
AGAGAAGTTGAAGAAACAAA+AGG 0.500916 2_3:+17885016 Msa0282120:CDS
CCTGAATCGGAACAAAGAAA+AGG 0.516491 2_3:+17884945 Msa0282120:CDS
GGAACAAAGAAAAGGAAAGC+TGG 0.520124 2_3:+17884953 Msa0282120:CDS
AGTTGAAGAAACAAAAGGAC+AGG 0.532305 2_3:+17885021 Msa0282120:CDS
AAAAGGAAAGCTGGATCATG+AGG 0.532487 2_3:+17884962 Msa0282120:CDS
AAGAAAATGAATGATCTACT+TGG 0.551967 2_3:+17885053 Msa0282120:CDS
ACGGTTTATCCATCAAAAGA+AGG 0.566324 2_3:+17884888 Msa0282120:CDS
ACATAACTGATGACTTGAAG+AGG 0.573872 2_3:+17884367 Msa0282120:CDS
AGAAGGAGTCCAGATGGTGT+TGG 0.590630 2_3:+17884905 Msa0282120:CDS
ACCGAAAAGAAGTCGATGAG+AGG 0.615793 2_3:+17885156 Msa0282120:CDS
ATCAAAAGAAGGAGTCCAGA+TGG 0.615817 2_3:+17884899 Msa0282120:CDS
TGCATCAATTCCACAAAGTG+TGG 0.616904 2_3:-17884829 None:intergenic
TGAAAACAATGATGGAGCAA+CGG 0.627126 2_3:+17884869 Msa0282120:CDS
ACATTCAACAAAAGGAAGCA+TGG 0.630577 2_3:+17884396 Msa0282120:CDS
AAAGGAAAGCTGGATCATGA+GGG 0.661580 2_3:+17884963 Msa0282120:CDS
CTTTGTGGAATTGATGCATG+TGG 0.684212 2_3:+17884834 Msa0282120:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGTGTTCTTTATAGAAATAA+AGG - chr2_3:17884439-17884458 None:intergenic 20.0%
!!! TTTCATATTTTTAGTATCCA+TGG - chr2_3:17884529-17884548 None:intergenic 20.0%
!! TCATATTGATAAATTCCTAA+TGG - chr2_3:17884565-17884584 None:intergenic 20.0%
!! TCATAACTGATATGTTATTA+TGG - chr2_3:17884665-17884684 None:intergenic 20.0%
!! GAAAATAACGATAATTTCAA+AGG - chr2_3:17884688-17884707 None:intergenic 20.0%
!! GAAAAAATTTATACTATAGC+TGG - chr2_3:17884730-17884749 None:intergenic 20.0%
!! AGTGTTCTTTATAGAAATAA+AGG - chr2_3:17884439-17884458 None:intergenic 20.0%
!!! TTTCATATTTTTAGTATCCA+TGG - chr2_3:17884529-17884548 None:intergenic 20.0%
!! TCATATTGATAAATTCCTAA+TGG - chr2_3:17884565-17884584 None:intergenic 20.0%
!! TCATAACTGATATGTTATTA+TGG - chr2_3:17884665-17884684 None:intergenic 20.0%
!! GAAAATAACGATAATTTCAA+AGG - chr2_3:17884688-17884707 None:intergenic 20.0%
!! GAAAAAATTTATACTATAGC+TGG - chr2_3:17884730-17884749 None:intergenic 20.0%
!!! GTTTTTTTTTATCACTACCA+TGG + chr2_3:17884509-17884528 Msa0282120:intron 25.0%
! ATCTATGATGAAAACAATGA+TGG + chr2_3:17884861-17884880 Msa0282120:CDS 25.0%
!! ATCATTCATTTTCTTCTTCT+TGG - chr2_3:17885048-17885067 None:intergenic 25.0%
! AAGAAAATGAATGATCTACT+TGG + chr2_3:17885053-17885072 Msa0282120:CDS 25.0%
!!! GTTTTTTTTTATCACTACCA+TGG + chr2_3:17884509-17884528 Msa0282120:intron 25.0%
! ATCTATGATGAAAACAATGA+TGG + chr2_3:17884861-17884880 Msa0282120:CDS 25.0%
!! ATCATTCATTTTCTTCTTCT+TGG - chr2_3:17885048-17885067 None:intergenic 25.0%
! AAGAAAATGAATGATCTACT+TGG + chr2_3:17885053-17885072 Msa0282120:CDS 25.0%
!! GTTTTGTTGTGTTTTTCAAC+AGG + chr2_3:17884780-17884799 Msa0282120:intron 30.0%
CTTCTCTTTCGATTTCATTA+TGG - chr2_3:17885003-17885022 None:intergenic 30.0%
AGAGAAGTTGAAGAAACAAA+AGG + chr2_3:17885016-17885035 Msa0282120:CDS 30.0%
ATTCATACTGGTGAATTTGA+TGG + chr2_3:17885083-17885102 Msa0282120:CDS 30.0%
GAAGATCTATCATTGCTTAT+TGG + chr2_3:17885128-17885147 Msa0282120:CDS 30.0%
!! GTTTTGTTGTGTTTTTCAAC+AGG + chr2_3:17884780-17884799 Msa0282120:intron 30.0%
CTTCTCTTTCGATTTCATTA+TGG - chr2_3:17885003-17885022 None:intergenic 30.0%
AGAGAAGTTGAAGAAACAAA+AGG + chr2_3:17885016-17885035 Msa0282120:CDS 30.0%
ATTCATACTGGTGAATTTGA+TGG + chr2_3:17885083-17885102 Msa0282120:CDS 30.0%
GAAGATCTATCATTGCTTAT+TGG + chr2_3:17885128-17885147 Msa0282120:CDS 30.0%
ACATAACTGATGACTTGAAG+AGG + chr2_3:17884367-17884386 Msa0282120:CDS 35.0%
GGAAGACAACATTCAACAAA+AGG + chr2_3:17884388-17884407 Msa0282120:CDS 35.0%
ACATTCAACAAAAGGAAGCA+TGG + chr2_3:17884396-17884415 Msa0282120:CDS 35.0%
AAGTTGACTGCATAACCATT+AGG + chr2_3:17884547-17884566 Msa0282120:intron 35.0%
! TGAAAACAATGATGGAGCAA+CGG + chr2_3:17884869-17884888 Msa0282120:CDS 35.0%
ACGGTTTATCCATCAAAAGA+AGG + chr2_3:17884888-17884907 Msa0282120:CDS 35.0%
AGTTGAAGAAACAAAAGGAC+AGG + chr2_3:17885021-17885040 Msa0282120:CDS 35.0%
CTTGGTGAATTCATTCATAC+TGG + chr2_3:17885071-17885090 Msa0282120:CDS 35.0%
ACATAACTGATGACTTGAAG+AGG + chr2_3:17884367-17884386 Msa0282120:CDS 35.0%
GGAAGACAACATTCAACAAA+AGG + chr2_3:17884388-17884407 Msa0282120:CDS 35.0%
ACATTCAACAAAAGGAAGCA+TGG + chr2_3:17884396-17884415 Msa0282120:CDS 35.0%
AAGTTGACTGCATAACCATT+AGG + chr2_3:17884547-17884566 Msa0282120:intron 35.0%
! TGAAAACAATGATGGAGCAA+CGG + chr2_3:17884869-17884888 Msa0282120:CDS 35.0%
ACGGTTTATCCATCAAAAGA+AGG + chr2_3:17884888-17884907 Msa0282120:CDS 35.0%
AGTTGAAGAAACAAAAGGAC+AGG + chr2_3:17885021-17885040 Msa0282120:CDS 35.0%
CTTGGTGAATTCATTCATAC+TGG + chr2_3:17885071-17885090 Msa0282120:CDS 35.0%
AGTGAAATCACCACACTTTG+TGG + chr2_3:17884819-17884838 Msa0282120:CDS 40.0%
TGCATCAATTCCACAAAGTG+TGG - chr2_3:17884832-17884851 None:intergenic 40.0%
CTTTGTGGAATTGATGCATG+TGG + chr2_3:17884834-17884853 Msa0282120:CDS 40.0%
! ATCTGGACTCCTTCTTTTGA+TGG - chr2_3:17884900-17884919 None:intergenic 40.0%
ATCAAAAGAAGGAGTCCAGA+TGG + chr2_3:17884899-17884918 Msa0282120:CDS 40.0%
TAAACTTGTCCAACACCATC+TGG - chr2_3:17884917-17884936 None:intergenic 40.0%
! AGATGGTGTTGGACAAGTTT+AGG + chr2_3:17884916-17884935 Msa0282120:CDS 40.0%
GTTTAGGAGCTTTCCTGAAT+CGG + chr2_3:17884932-17884951 Msa0282120:CDS 40.0%
! CCTTTTCTTTGTTCCGATTC+AGG - chr2_3:17884948-17884967 None:intergenic 40.0%
CCTGAATCGGAACAAAGAAA+AGG + chr2_3:17884945-17884964 Msa0282120:CDS 40.0%
GGAACAAAGAAAAGGAAAGC+TGG + chr2_3:17884953-17884972 Msa0282120:CDS 40.0%
AAAAGGAAAGCTGGATCATG+AGG + chr2_3:17884962-17884981 Msa0282120:CDS 40.0%
AAAGGAAAGCTGGATCATGA+GGG + chr2_3:17884963-17884982 Msa0282120:CDS 40.0%
!!! TCCTCTCATCGACTTCTTTT+CGG - chr2_3:17885160-17885179 None:intergenic 40.0%
AGTGAAATCACCACACTTTG+TGG + chr2_3:17884819-17884838 Msa0282120:CDS 40.0%
TGCATCAATTCCACAAAGTG+TGG - chr2_3:17884832-17884851 None:intergenic 40.0%
CTTTGTGGAATTGATGCATG+TGG + chr2_3:17884834-17884853 Msa0282120:CDS 40.0%
! ATCTGGACTCCTTCTTTTGA+TGG - chr2_3:17884900-17884919 None:intergenic 40.0%
ATCAAAAGAAGGAGTCCAGA+TGG + chr2_3:17884899-17884918 Msa0282120:CDS 40.0%
TAAACTTGTCCAACACCATC+TGG - chr2_3:17884917-17884936 None:intergenic 40.0%
! AGATGGTGTTGGACAAGTTT+AGG + chr2_3:17884916-17884935 Msa0282120:CDS 40.0%
GTTTAGGAGCTTTCCTGAAT+CGG + chr2_3:17884932-17884951 Msa0282120:CDS 40.0%
! CCTTTTCTTTGTTCCGATTC+AGG - chr2_3:17884948-17884967 None:intergenic 40.0%
CCTGAATCGGAACAAAGAAA+AGG + chr2_3:17884945-17884964 Msa0282120:CDS 40.0%
GGAACAAAGAAAAGGAAAGC+TGG + chr2_3:17884953-17884972 Msa0282120:CDS 40.0%
AAAAGGAAAGCTGGATCATG+AGG + chr2_3:17884962-17884981 Msa0282120:CDS 40.0%
AAAGGAAAGCTGGATCATGA+GGG + chr2_3:17884963-17884982 Msa0282120:CDS 40.0%
!!! TCCTCTCATCGACTTCTTTT+CGG - chr2_3:17885160-17885179 None:intergenic 40.0%
ATTTCCCTCAACTCGCTCAT+GGG + chr2_3:17884758-17884777 Msa0282120:intron 45.0%
! AAAACCCATGAGCGAGTTGA+GGG - chr2_3:17884765-17884784 None:intergenic 45.0%
ACCGAAAAGAAGTCGATGAG+AGG + chr2_3:17885156-17885175 Msa0282120:CDS 45.0%
ATTTCCCTCAACTCGCTCAT+GGG + chr2_3:17884758-17884777 Msa0282120:intron 45.0%
! AAAACCCATGAGCGAGTTGA+GGG - chr2_3:17884765-17884784 None:intergenic 45.0%
ACCGAAAAGAAGTCGATGAG+AGG + chr2_3:17885156-17885175 Msa0282120:CDS 45.0%
CATTTCCCTCAACTCGCTCA+TGG + chr2_3:17884757-17884776 Msa0282120:intron 50.0%
CAAAACCCATGAGCGAGTTG+AGG - chr2_3:17884766-17884785 None:intergenic 50.0%
AGAAGGAGTCCAGATGGTGT+TGG + chr2_3:17884905-17884924 Msa0282120:CDS 50.0%
CATTTCCCTCAACTCGCTCA+TGG + chr2_3:17884757-17884776 Msa0282120:intron 50.0%
CAAAACCCATGAGCGAGTTG+AGG - chr2_3:17884766-17884785 None:intergenic 50.0%
AGAAGGAGTCCAGATGGTGT+TGG + chr2_3:17884905-17884924 Msa0282120:CDS 50.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 17884339 17885199 17884339 ID=Msa0282120;Name=Msa0282120
chr2_3 mRNA 17884339 17885199 17884339 ID=Msa0282120-mRNA-1;Parent=Msa0282120;Name=Msa0282120-mRNA-1;_AED=0.29;_eAED=0.29;_QI=0|0|0|1|1|1|2|0|158
chr2_3 exon 17884339 17884417 17884339 ID=Msa0282120-mRNA-1:exon:7326;Parent=Msa0282120-mRNA-1
chr2_3 exon 17884802 17885199 17884802 ID=Msa0282120-mRNA-1:exon:7327;Parent=Msa0282120-mRNA-1
chr2_3 CDS 17884339 17884417 17884339 ID=Msa0282120-mRNA-1:cds;Parent=Msa0282120-mRNA-1
chr2_3 CDS 17884802 17885199 17884802 ID=Msa0282120-mRNA-1:cds;Parent=Msa0282120-mRNA-1
Gene Sequence

>Msa0282120

ATGGCTGATAAAAATATGAGAGTTGACTACATAACTGATGACTTGAAGAGGAAGACAACATTCAACAAAAGGAAGCATGGTATAACTAAGAAGATTAGTGAAATCACCACACTTTGTGGAATTGATGCATGTGGTATAATCTATGATGAAAACAATGATGGAGCAACGGTTTATCCATCAAAAGAAGGAGTCCAGATGGTGTTGGACAAGTTTAGGAGCTTTCCTGAATCGGAACAAAGAAAAGGAAAGCTGGATCATGAGGGCTTTTTGAGACAATCCATAATGAAATCGAAAGAGAAGTTGAAGAAACAAAAGGACAGGTCCAAGAAGAAGAAAATGAATGATCTACTTGGTGAATTCATTCATACTGGTGAATTTGATGGAAATGTGAGCGAGAATGATCTCGAAGATCTATCATTGCTTATTGGTGATTACCGAAAAGAAGTCGATGAGAGGATCGAATCAAGTCAAACCTGA

Protein sequence

>Msa0282120

MADKNMRVDYITDDLKRKTTFNKRKHGITKKISEITTLCGIDACGIIYDENNDGATVYPSKEGVQMVLDKFRSFPESEQRKGKLDHEGFLRQSIMKSKEKLKKQKDRSKKKKMNDLLGEFIHTGEFDGNVSENDLEDLSLLIGDYRKEVDERIESSQT*