AlfalfaGEDB | Msa0285800

M. sativa cultivar zhongmu-4 / Msa0285800

KEGG: K12412
GO: -
PFAM: PF00319.21

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0285800	RHN73765.1	76.733	202	31	3	2	203	4	189	6.79e-95	285

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0285800	sp\|Q4PSU4\|AGL61_ARATH	29.032	155	100	6	45	194	65	214	1.53e-12	67.8

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0285800	A0A396JBD2	76.733	202	31	3	2	203	4	189	3.24e-95	285

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TFs/TRs:

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Gene ID	Type	Classification
Msa0285800	TF	MADS-M-type

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0285800	MtrunA17_Chr2g0302091	76.733	202	31	3	2	203	4	189	6.24e-99	285
Msa0285800	MtrunA17_Chr3g0093131	62.105	190	60	6	17	204	1	180	3.54e-65	199
Msa0285800	MtrunA17_Chr8g0345001	50.000	178	82	5	31	204	3	177	6.47e-46	150
Msa0285800	MtrunA17_Chr4g0018951	34.559	136	75	3	63	197	31	153	2.54e-18	78.6
Msa0285800	MtrunA17_Chr2g0333981	37.037	162	90	6	43	197	5	161	5.80e-18	77.8
Msa0285800	MtrunA17_Chr8g0379021	34.810	158	94	6	43	197	10	161	3.14e-14	67.8
Msa0285800	MtrunA17_Chr8g0355091	33.529	170	89	8	31	191	2	156	1.17e-13	67.4
Msa0285800	MtrunA17_Chr1g0206991	34.177	158	92	7	44	194	7	159	7.74e-13	64.3
Msa0285800	MtrunA17_Chr2g0292791	32.370	173	93	8	31	194	2	159	8.03e-13	65.1
Msa0285800	MtrunA17_Chr1g0206701	33.962	159	85	7	44	191	7	156	9.25e-13	65.1

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0285800	AT2G24840.1	29.032	155	100	6	45	194	65	214	1.55e-13	67.8
Msa0285800	AT5G60440.1	30.573	157	97	6	45	194	9	160	3.44e-13	67.4

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Find 28 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGAACAAGTTCTATGCATG+TGG	0.639320	2_3:+25768701	Msa0285800:CDS
AATTTACGTGCAGCAAGCGA+AGG	0.589056	2_3:+25769007	Msa0285800:CDS
ACGATGAATGACAGCATCAC+AGG	0.499770	2_3:-25768729	None:intergenic
ATCCTCGGCCATCATATGTC+CGG	0.508496	2_3:-25768759	None:intergenic
ATGAAATTGCTGAAACCCTA+AGG	0.533334	2_3:+25768804	Msa0285800:CDS
ATGTCAATTTCCATGATTGG+TGG	0.630551	2_3:+25768912	Msa0285800:CDS
CGATGAATGACAGCATCACA+GGG	0.591570	2_3:-25768728	None:intergenic
GAACAACACTGAACAACACT+CGG	0.583442	2_3:+25768534	Msa0285800:CDS
GACATATGATGGCCGAGGAT+GGG	0.528388	2_3:+25768762	Msa0285800:CDS
GACCGGACATATGATGGCCG+AGG	0.586444	2_3:+25768757	Msa0285800:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATATCATGAAAGTGAGTGAT+TGG	+	chr2_3:25768295-25768314	Msa0285800:intron	30.0%
TATCATGAAAGTGAGTGATT+GGG	+	chr2_3:25768296-25768315	Msa0285800:intron	30.0%
TGATGTTGAATGTAACAAAC+TGG	+	chr2_3:25768441-25768460	Msa0285800:CDS	30.0%
GATGTTGAATGTAACAAACT+GGG	+	chr2_3:25768442-25768461	Msa0285800:CDS	30.0%
TAGAATTATTCCACCAATCA+TGG	-	chr2_3:25768925-25768944	None:intergenic	30.0%
ATATCATGAAAGTGAGTGAT+TGG	+	chr2_3:25768295-25768314	Msa0285800:intron	30.0%
TATCATGAAAGTGAGTGATT+GGG	+	chr2_3:25768296-25768315	Msa0285800:intron	30.0%
TGATGTTGAATGTAACAAAC+TGG	+	chr2_3:25768441-25768460	Msa0285800:CDS	30.0%
GATGTTGAATGTAACAAACT+GGG	+	chr2_3:25768442-25768461	Msa0285800:CDS	30.0%
TAGAATTATTCCACCAATCA+TGG	-	chr2_3:25768925-25768944	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_3	gene	25768221	25769036	25768221	ID=Msa0285800;Name=Msa0285800
chr2_3	mRNA	25768221	25769036	25768221	ID=Msa0285800-mRNA-1;Parent=Msa0285800;Name=Msa0285800-mRNA-1;_AED=0.29;_eAED=0.30;_QI=0\|0\|0\|0.5\|1\|1\|2\|0\|204
chr2_3	exon	25768221	25768224	25768221	ID=Msa0285800-mRNA-1:exon:9514;Parent=Msa0285800-mRNA-1
chr2_3	exon	25768426	25769036	25768426	ID=Msa0285800-mRNA-1:exon:9515;Parent=Msa0285800-mRNA-1
chr2_3	CDS	25768221	25768224	25768221	ID=Msa0285800-mRNA-1:cds;Parent=Msa0285800-mRNA-1
chr2_3	CDS	25768426	25769036	25768426	ID=Msa0285800-mRNA-1:cds;Parent=Msa0285800-mRNA-1

Gene Sequence

>Msa0285800

ATGGATAATTCATCATCAGTGATGTTGAATGTAACAAACTGGGACAACATGAATAAGAATTGTGAACTGTCTGAAAAGAACACTGCTAGGGCTTCTGAGAGAAAGAATGCTGGAACAACACTGAACAACACTCGGAAGAGAAAGATGCATGAAATCAAGAAAGAAGAAAAACTGAACGGTTCTGAGTTCTCAAGACGCAAACTCGGAATATTCAACAAAGCAATAGAGCTGTCAATTCTATGCCAATCAAAAACGGCACTCCTCGTCAAATCGCCGGACAAGAACAAGTTCTATGCATGTGGCTACCCCTGTGATGCTGTCATTCATCGTTTTCTGACCGGACATATGATGGCCGAGGATGGGAAAAAGAAGAAGCAAGATGATGAAATTGCTGAAACCCTAAGGCTTCAATATGAAGCACTTCAAGAAAAACTTGAAGAAGAGCAGGATACTTTGGAAGCTGTCAAAGTCACCGAGGCACAAAAGAGTGATGTCAATTTCCATGATTGGTGGAATAATTCTATTGATGATATGAATTTAGCATCTCTTGAAGACTTTAAGAATTCTTTGGAGAGTTTTAAGGGTAATTTACGTGCAGCAAGCGAAGGAAAGTAG

Protein sequence

>Msa0285800

MDNSSSVMLNVTNWDNMNKNCELSEKNTARASERKNAGTTLNNTRKRKMHEIKKEEKLNGSEFSRRKLGIFNKAIELSILCQSKTALLVKSPDKNKFYACGYPCDAVIHRFLTGHMMAEDGKKKKQDDEIAETLRLQYEALQEKLEEEQDTLEAVKVTEAQKSDVNFHDWWNNSIDDMNLASLEDFKNSLESFKGNLRAASEGK*