AlfalfaGEDB | Msa0289150

M. sativa cultivar zhongmu-4 / Msa0289150

KEGG: K07760
GO: GO:0000003 GO:0000082 GO:0000278 GO:0000307 GO:0000902 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004693 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006275 GO:0006464 GO:0006468 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0007049 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0008150 GO:0008152 GO:0008284 GO:0009058 GO:0009059 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009888 GO:0009889 GO:0009987 GO:0010016 GO:0010033 GO:0010103 GO:0010154 GO:0010374 GO:0010376 GO:0010389 GO:0010440 GO:0010444 GO:0010468 GO:0010556 GO:0010564 GO:0016043 GO:0016049 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019219 GO:0019222 GO:0019538 GO:0022402 GO:0022414 GO:0023052 GO:0030154 GO:0030332 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032875 GO:0032989 GO:0032991 GO:0034641 GO:0034645 GO:0036211 GO:0040007 GO:0042023 GO:0042127 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0044770 GO:0044772 GO:0044786 GO:0044843 GO:0046483 GO:0048316 GO:0048366 GO:0048367 GO:0048518 GO:0048522 GO:0048589 GO:0048608 GO:0048646 GO:0048731 GO:0048825 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051052 GO:0051171 GO:0051239 GO:0051716 GO:0051726 GO:0060255 GO:0060560 GO:0061458 GO:0061695 GO:0065007 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090329 GO:0090558 GO:0090626 GO:0090627 GO:0090698 GO:0097472 GO:0099402 GO:0140096 GO:1901360 GO:1901564 GO:1901576 GO:1901987 GO:1901990 GO:1902494 GO:1902554 GO:1902749 GO:1902806 GO:1902911 GO:1903047 GO:1990234 GO:2000026 GO:2000037 GO:2000112
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0289150	KEH19587.1	85.714	56	7	1	11	65	1	56	3.63e-25	100

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0289150	sp\|Q2V419\|CKB12_ARATH	50.725	69	29	1	1	64	166	234	1.49e-15	71.2

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0289150	MtrunA17_Chr8g0360761	91.429	35	3	0	10	44	74	108	1.74e-18	74.3
Msa0289150	MtrunA17_Chr4g0002001	61.224	49	19	0	16	64	186	234	2.66e-15	67.8
Msa0289150	MtrunA17_Chr3g0093391	57.143	49	21	0	16	64	203	251	3.60e-15	67.8
Msa0289150	MtrunA17_Chr3g0093571	57.143	49	21	0	16	64	205	253	6.72e-15	67.0
Msa0289150	MtrunA17_Chr6g0486031	60.000	50	19	1	11	59	275	324	8.26e-15	66.6
Msa0289150	MtrunA17_Chr4g0051261	59.184	49	20	0	16	64	169	217	1.57e-14	65.5
Msa0289150	MtrunA17_Chr2g0293491	59.184	49	20	0	16	64	169	217	4.15e-14	64.3
Msa0289150	MtrunA17_Chr3g0130781	58.537	41	17	0	16	56	196	236	6.63e-14	63.9
Msa0289150	MtrunA17_Chr3g0102571	62.500	40	15	0	16	55	247	286	7.20e-14	63.9
Msa0289150	MtrunA17_Chr8g0390341	56.000	50	21	1	11	59	288	337	1.00e-13	63.5

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0289150	AT2G38620.3	50.725	69	29	1	1	64	166	234	5.03e-17	72.0
Msa0289150	AT2G38620.1	50.725	69	29	1	1	64	166	234	5.03e-17	72.0
Msa0289150	AT2G38620.4	50.725	69	29	1	1	64	104	172	8.36e-17	71.2
Msa0289150	AT2G38620.2	50.725	69	29	1	1	64	166	234	1.52e-16	71.2
Msa0289150	AT3G54180.1	63.265	49	18	0	16	64	184	232	3.00e-15	67.8
Msa0289150	AT3G48750.1	57.143	49	21	0	16	64	169	217	3.31e-14	65.1
Msa0289150	AT3G59790.1	60.000	40	16	0	16	55	226	265	8.95e-14	63.9
Msa0289150	AT1G76540.1	57.143	49	21	0	16	64	187	235	1.01e-13	63.5
Msa0289150	AT4G11330.1	60.000	40	16	0	16	55	209	248	1.05e-13	63.9
Msa0289150	AT5G63610.1	62.500	40	15	0	16	55	201	240	1.15e-13	63.9

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Find 16 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AATATCGCAGCCAATATCTG+CGG	0.719781	2_3:+35418012	None:intergenic
ACAGAAGCAATAACCTTCAA+TGG	0.414424	2_3:+35418194	None:intergenic
ACTCAACTGCAGTTGATGTC+TGG	0.472124	2_3:-35418098	Msa0289150:CDS
AGATATTGGCTGCGATATTT+TGG	0.233067	2_3:-35418008	Msa0289150:CDS
AGCAATAACCTTCAATGGTT+TGG	0.308387	2_3:+35418199	None:intergenic
AGGCTCCAACCTCTGTTCAT+TGG	0.396329	2_3:-35418045	Msa0289150:CDS
AGTTCAGGTGCTTTATAGCA+AGG	0.509440	2_3:+35418141	None:intergenic
ATATCTTCACCAATGAACAG+AGG	0.733276	2_3:+35418036	None:intergenic
ATCAACTGCAGTTGAGTAAT+TGG	0.293246	2_3:+35418104	None:intergenic
ATGTTACTCCAAACCATTGA+AGG	0.442393	2_3:-35418207	Msa0289150:intron

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATGTTACTCCAAACCATTGA+AGG	-	chr2_3:35418013-35418032	Msa0289150:CDS	35.0%
AGCAATAACCTTCAATGGTT+TGG	+	chr2_3:35418024-35418043	None:intergenic	35.0%
ACAGAAGCAATAACCTTCAA+TGG	+	chr2_3:35418029-35418048	None:intergenic	35.0%
ATCAACTGCAGTTGAGTAAT+TGG	+	chr2_3:35418119-35418138	None:intergenic	35.0%
ATATCTTCACCAATGAACAG+AGG	+	chr2_3:35418187-35418206	None:intergenic	35.0%
TGAAGATATACCGCAGATAT+TGG	-	chr2_3:35418198-35418217	Msa0289150:intron	35.0%
AGTTCAGGTGCTTTATAGCA+AGG	+	chr2_3:35418082-35418101	None:intergenic	40.0%
GTAGAACCCATCAAAAGTTC+AGG	+	chr2_3:35418097-35418116	None:intergenic	40.0%
AATATCGCAGCCAATATCTG+CGG	+	chr2_3:35418211-35418230	None:intergenic	40.0%
CTTCACCAATGAACAGAGGT+TGG	+	chr2_3:35418183-35418202	None:intergenic	45.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_3	gene	35418005	35418237	35418005	ID=Msa0289150;Name=Msa0289150
chr2_3	mRNA	35418005	35418237	35418005	ID=Msa0289150-mRNA-1;Parent=Msa0289150;Name=Msa0289150-mRNA-1;_AED=0.48;_eAED=0.48;_QI=0\|0\|0\|0.5\|0\|0.5\|2\|0\|65
chr2_3	exon	35418208	35418237	35418208	ID=Msa0289150-mRNA-1:exon:11471;Parent=Msa0289150-mRNA-1
chr2_3	exon	35418005	35418172	35418005	ID=Msa0289150-mRNA-1:exon:11470;Parent=Msa0289150-mRNA-1
chr2_3	CDS	35418208	35418237	35418208	ID=Msa0289150-mRNA-1:cds;Parent=Msa0289150-mRNA-1
chr2_3	CDS	35418005	35418172	35418005	ID=Msa0289150-mRNA-1:cds;Parent=Msa0289150-mRNA-1

Gene Sequence	>Msa0289150 ATGAATAAATGTTACTCCAAACCATTGAAGATGGGTAATCCTTGCTATAAAGCACCTGAACTTTTGATGGGTTCTACCAATTACTCAACTGCAGTTGATGTCTGGTCTATGGGATGCATATTTGCTGAGATGCTTAGGCTCCAACCTCTGTTCATTGGTGAAGATATACCGCAGATATTGGCTGCGATATTTTGGTAA
Protein sequence	>Msa0289150 MNKCYSKPLKMGNPCYKAPELLMGSTNYSTAVDVWSMGCIFAEMLRLQPLFIGEDIPQILAAIFW*