AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0301290

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0301290 MtrunA17_Chr2g0282511 87.963 324 21 5 1 321 1 309 5.07e-163 456
Msa0301290 MtrunA17_Chr4g0047001 45.337 386 119 18 14 321 13 384 5.67e-63 204
Msa0301290 MtrunA17_Chr4g0031411 43.353 173 70 5 41 210 72 219 3.55e-32 122
Msa0301290 MtrunA17_Chr2g0328021 49.153 118 44 2 10 127 29 130 7.76e-32 120
Msa0301290 MtrunA17_Chr8g0374251 71.429 70 20 0 39 108 63 132 1.25e-31 121
Msa0301290 MtrunA17_Chr4g0010151 68.132 91 26 2 41 128 79 169 2.08e-31 120
Msa0301290 MtrunA17_Chr8g0374111 57.955 88 28 1 40 127 25 103 8.58e-31 115
Msa0301290 MtrunA17_Chr4g0030051 74.242 66 17 0 31 96 28 93 1.55e-30 117
Msa0301290 MtrunA17_Chr1g0188851 55.670 97 32 2 15 100 3 99 2.31e-30 116
Msa0301290 MtrunA17_Chr2g0326001 52.727 110 46 2 30 139 26 129 2.85e-30 116
Msa0301290 MtrunA17_Chr1g0177251 48.800 125 59 2 41 163 9 130 3.93e-30 115
Msa0301290 MtrunA17_Chr7g0223311 68.571 70 22 0 37 106 70 139 3.95e-30 117
Msa0301290 MtrunA17_Chr8g0367161 67.647 68 22 0 35 102 23 90 1.08e-29 115
Msa0301290 MtrunA17_Chr8g0346551 55.435 92 30 1 31 122 11 91 1.21e-29 114
Msa0301290 MtrunA17_Chr8g0341671 72.727 66 16 1 40 105 16 79 1.75e-29 112
Msa0301290 MtrunA17_Chr3g0127201 76.562 64 12 1 39 102 22 82 2.21e-29 114
Msa0301290 MtrunA17_Chr7g0237921 80.702 57 11 0 40 96 72 128 3.99e-29 114
Msa0301290 MtrunA17_Chr2g0282051 64.865 74 25 1 27 100 29 101 4.42e-29 112
Msa0301290 MtrunA17_Chr3g0126201 62.338 77 29 0 29 105 36 112 1.98e-28 112
Msa0301290 MtrunA17_Chr5g0411951 79.630 54 11 0 41 94 40 93 2.84e-28 112
Msa0301290 MtrunA17_Chr2g0282441 75.410 61 15 0 35 95 16 76 4.78e-28 111
Msa0301290 MtrunA17_Chr3g0117601 72.131 61 17 0 34 94 51 111 9.46e-28 110
Msa0301290 MtrunA17_Chr4g0047761 70.270 74 21 1 28 101 56 128 1.52e-27 109
Msa0301290 MtrunA17_Chr2g0308011 86.000 50 7 0 43 92 36 85 1.52e-27 107
Msa0301290 MtrunA17_Chr4g0061761 81.132 53 10 0 42 94 38 90 1.67e-27 109
Msa0301290 MtrunA17_Chr6g0453321 54.321 81 33 1 20 96 108 188 1.68e-25 106
Msa0301290 MtrunA17_Chr3g0092111 62.121 66 25 0 38 103 130 195 2.18e-25 105
Msa0301290 MtrunA17_Chr2g0283811 59.677 62 25 0 34 95 37 98 1.49e-24 97.4
Msa0301290 MtrunA17_Chr7g0217581 57.895 76 25 1 30 98 211 286 1.97e-24 103
Msa0301290 MtrunA17_Chr7g0254161 49.495 99 49 1 35 133 92 189 2.27e-24 102
Msa0301290 MtrunA17_Chr4g0042481 53.333 75 35 0 34 108 112 186 2.56e-24 102
Msa0301290 MtrunA17_Chr8g0355261 48.810 84 39 1 34 117 108 187 3.48e-24 102
Msa0301290 MtrunA17_Chr5g0417561 44.037 109 61 0 13 121 88 196 5.79e-24 100
Msa0301290 MtrunA17_Chr6g0460721 40.000 130 77 1 24 153 69 197 8.93e-24 99.4
Msa0301290 MtrunA17_Chr6g0460731 50.685 73 36 0 24 96 76 148 4.51e-23 98.2
Msa0301290 MtrunA17_Chr5g0417571 50.000 82 39 1 15 96 111 190 6.62e-23 97.1
Msa0301290 MtrunA17_Chr5g0417551 40.625 128 71 1 13 140 96 218 1.73e-22 96.7
Msa0301290 MtrunA17_Chr5g0417601 48.235 85 42 1 15 99 113 195 7.59e-22 94.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0301290 AT1G28310.1 41.883 308 126 13 44 321 27 311 2.95e-44 154
Msa0301290 AT1G28310.2 41.883 308 126 13 44 321 51 335 3.23e-44 154
Msa0301290 AT3G55370.1 53.333 105 49 0 4 108 36 140 2.63e-34 128
Msa0301290 AT3G55370.3 53.333 105 49 0 4 108 81 185 3.33e-34 129
Msa0301290 AT3G55370.2 53.333 105 49 0 4 108 36 140 4.88e-34 128
Msa0301290 AT1G51700.1 44.025 159 81 5 31 186 21 174 2.83e-33 122
Msa0301290 AT5G60200.1 42.073 164 65 4 38 201 49 182 1.60e-31 119
Msa0301290 AT3G45610.1 41.463 164 75 5 41 202 37 181 5.74e-31 117
Msa0301290 AT1G07640.2 60.000 85 26 1 28 104 53 137 7.73e-31 119
Msa0301290 AT1G07640.1 78.689 61 13 0 44 104 21 81 8.31e-31 117
Msa0301290 AT2G28810.2 81.034 58 11 0 38 95 71 128 8.80e-31 118
Msa0301290 AT1G07640.3 60.000 85 26 1 28 104 61 145 1.02e-30 119
Msa0301290 AT2G28810.1 81.034 58 11 0 38 95 88 145 1.18e-30 119
Msa0301290 AT5G65590.1 60.000 85 34 0 19 103 16 100 1.79e-30 117
Msa0301290 AT3G50410.1 74.603 63 16 0 40 102 26 88 2.07e-30 115
Msa0301290 AT2G37590.1 65.753 73 25 0 28 100 73 145 3.98e-30 117
Msa0301290 AT5G02460.1 87.037 54 7 0 43 96 94 147 1.89e-29 116
Msa0301290 AT3G21270.1 50.962 104 50 1 44 147 29 131 1.89e-29 112
Msa0301290 AT1G21340.1 58.065 93 32 2 16 101 8 100 4.20e-29 112
Msa0301290 AT5G66940.1 46.512 129 61 4 27 149 13 139 9.13e-29 110
Msa0301290 AT2G28510.1 39.444 180 83 5 42 213 46 207 2.06e-28 111
Msa0301290 AT4G38000.1 78.947 57 12 0 41 97 38 94 2.78e-28 110
Msa0301290 AT5G60850.1 73.770 61 16 0 40 100 47 107 6.55e-28 110
Msa0301290 AT4G24060.1 66.667 63 21 0 34 96 43 105 3.18e-27 109
Msa0301290 AT5G62940.1 69.355 62 19 0 33 94 62 123 1.39e-26 108
Msa0301290 AT3G61850.2 66.667 66 19 1 37 102 55 117 1.48e-26 106
Msa0301290 AT3G61850.3 66.667 66 19 1 37 102 55 117 1.48e-26 106
Msa0301290 AT3G61850.1 70.968 62 15 1 41 102 71 129 1.72e-26 106
Msa0301290 AT3G61850.5 66.667 66 19 1 37 102 55 117 1.72e-26 107
Msa0301290 AT3G52440.1 74.194 62 14 1 46 105 27 88 2.20e-26 105
Msa0301290 AT3G61850.4 70.968 62 15 1 41 102 71 129 2.31e-26 106
Msa0301290 AT3G52440.2 74.194 62 14 1 46 105 46 107 3.44e-26 105
Msa0301290 AT4G00940.1 61.644 73 24 1 24 96 52 120 8.01e-26 104
Msa0301290 AT4G00940.3 61.644 73 24 1 24 96 52 120 8.01e-26 104
Msa0301290 AT4G00940.2 61.644 73 24 1 24 96 52 120 8.01e-26 104
Msa0301290 AT2G46590.1 71.930 57 16 0 35 91 59 115 9.31e-26 105
Msa0301290 AT2G46590.2 71.930 57 16 0 35 91 71 127 1.14e-25 105
Msa0301290 AT1G64620.1 69.492 59 18 0 34 92 39 97 1.69e-25 105
Msa0301290 AT3G47500.1 71.930 57 16 0 42 98 108 164 2.64e-25 105
Msa0301290 AT5G62430.1 62.903 62 23 0 38 99 48 109 7.23e-25 102
Msa0301290 AT5G39660.3 65.574 61 21 0 38 98 132 192 1.52e-24 103
Msa0301290 AT5G39660.1 65.574 61 21 0 38 98 132 192 1.52e-24 103
Msa0301290 AT5G39660.2 65.574 61 21 0 38 98 132 192 1.52e-24 103
Msa0301290 AT1G26790.1 47.573 103 52 2 20 122 88 188 2.75e-24 102
Msa0301290 AT1G47655.1 72.549 51 14 0 42 92 27 77 7.39e-24 97.4
Msa0301290 AT4G21050.1 76.000 50 12 0 46 95 26 75 1.37e-23 96.7
Msa0301290 AT1G29160.1 56.923 65 28 0 37 101 55 119 2.15e-23 95.1
Msa0301290 AT2G34140.1 56.923 65 28 0 37 101 51 115 2.66e-23 94.7
Msa0301290 AT1G69570.1 56.716 67 29 0 38 104 126 192 1.02e-22 97.8
Msa0301290 AT4G21040.1 47.619 105 37 3 21 125 12 98 1.97e-22 94.0
Msa0301290 AT4G21080.1 42.857 105 47 2 21 125 12 103 1.73e-21 92.0
Msa0301290 AT4G21030.1 60.870 46 18 0 46 91 23 68 6.13e-13 67.0

Find 78 sgRNAs with CRISPR-Local

Find 204 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAATCCTTAGTTCAAGTTTA+AGG 0.109986 2_3:+57170541 Msa0301290:three_prime_UTR
GGGCTTGGAATTTAACAATT+TGG 0.140338 2_3:+57170330 Msa0301290:CDS
GCAAAGCTTGCAAACGTTAT+TGG 0.143992 2_3:+57169771 Msa0301290:CDS
ACTCTTCAAGGATTCTATTT+TGG 0.149882 2_3:+57169309 Msa0301290:five_prime_UTR
AGGACTAATATTATGATTTA+AGG 0.162696 2_3:-57169963 None:intergenic
CACCAGCTTCTTCACTTTAC+TGG 0.188996 2_3:+57170422 Msa0301290:CDS
GGCTTGGAATTTAACAATTT+GGG 0.228469 2_3:+57170331 Msa0301290:CDS
GTTTAAGGTTATCTTTAATT+TGG 0.229380 2_3:+57170556 Msa0301290:three_prime_UTR
AATGATCAAGGTTCTAATAT+TGG 0.267697 2_3:+57170478 Msa0301290:CDS
TGTTGTCTGTATACTCTTCA+AGG 0.308452 2_3:+57169297 Msa0301290:five_prime_UTR
AATAGATGATGTTAAGATAA+TGG 0.329856 2_3:+57169625 Msa0301290:CDS
GCTTCAAGGGTTTCAAATAT+AGG 0.337429 2_3:-57169256 None:intergenic
TGTATAACCATTATGATCAT+TGG 0.338919 2_3:-57170146 None:intergenic
GGACAAGAGGTGGTACTCTT+AGG 0.348242 2_3:+57169792 Msa0301290:CDS
CAGATGAATACCATTGGATT+AGG 0.360977 2_3:+57170094 Msa0301290:CDS
ACAATCTAACTGAGGTTTGC+TGG 0.378078 2_3:-57170804 None:intergenic
CATCATCAGCTATTGGTGTT+TGG 0.379698 2_3:+57170455 Msa0301290:CDS
TCTAGAATCTCAACAAGTTC+AGG 0.387293 2_3:+57170055 Msa0301290:CDS
ATAACCTTAAACTTGAACTA+AGG 0.401805 2_3:-57170545 None:intergenic
GATCATAATGGTTATACAAA+TGG 0.418978 2_3:+57170151 Msa0301290:CDS
ATTATGATCATTGGCTTCGC+TGG 0.424556 2_3:-57170137 None:intergenic
CTTAGGAATGTTCCTGTTGG+TGG 0.434008 2_3:+57169809 Msa0301290:CDS
GCAGTTGGTCACTAGCTTCA+AGG 0.440834 2_3:-57169270 None:intergenic
GAATTTGAAGAAGTGGTGTT+TGG 0.453066 2_3:-57169938 None:intergenic
ACAGCATCATCATCAGCTAT+TGG 0.454258 2_3:+57170448 Msa0301290:CDS
TTGGGGAATGGAAGTGAGAA+AGG 0.465690 2_3:+57170349 Msa0301290:CDS
CTTCTGTAACCACCACCAAC+AGG 0.474357 2_3:-57169821 None:intergenic
ATTGGTGTTTGGAATGATCA+AGG 0.474683 2_3:+57170466 Msa0301290:CDS
GAATTTAACAATTTGGGGAA+TGG 0.481892 2_3:+57170337 Msa0301290:CDS
TAAGGTTGAATTTGAAGAAG+TGG 0.481982 2_3:-57169945 None:intergenic
CAACATAAATTCATGAATGA+TGG 0.486443 2_3:+57170292 Msa0301290:CDS
CAAGCTTTGCAGAAATGTCT+TGG 0.496012 2_3:-57169758 None:intergenic
CTGATTAGTGGTTGGAGGGA+TGG 0.496794 2_3:-57169369 None:intergenic
CAGCCTCAGATGAATACCAT+TGG 0.500398 2_3:+57170088 Msa0301290:CDS
AAAGGAATGAAGAAAGATGA+AGG 0.500974 2_3:+57170367 Msa0301290:CDS
GAAGAAAGATGAAGGAGAGA+TGG 0.502257 2_3:+57170375 Msa0301290:CDS
TGAGCTAGAGGAGAGTACTT+TGG 0.503240 2_3:+57170754 Msa0301290:three_prime_UTR
GGCATAACTGATGCATTACT+TGG 0.503446 2_3:-57170244 None:intergenic
GAGATATTGAACCTTGAGAA+TGG 0.505952 2_3:-57170034 None:intergenic
CAGTTGGTCACTAGCTTCAA+GGG 0.509480 2_3:-57169269 None:intergenic
AACCTGTGGCCACTGATTAG+TGG 0.512866 2_3:-57169381 None:intergenic
GAGAGGTAAAGGTGAGCTAG+AGG 0.512900 2_3:+57170742 Msa0301290:three_prime_UTR
GGCCACTGATTAGTGGTTGG+AGG 0.514127 2_3:-57169374 None:intergenic
AGAAGAATTAGTAGTATCAA+TGG 0.516034 2_3:-57169897 None:intergenic
AGGAATGTTCCTGTTGGTGG+TGG 0.516563 2_3:+57169812 Msa0301290:CDS
TTGCTGGATGCAGCTTACAT+AGG 0.517627 2_3:-57170788 None:intergenic
AGCGAAGCCAATGATCATAA+TGG 0.521310 2_3:+57170139 Msa0301290:CDS
ATGTGAATCTTCCATTCTCA+AGG 0.533312 2_3:+57170023 Msa0301290:CDS
CTTGATAAACCATAAAACAT+AGG 0.540160 2_3:-57169983 None:intergenic
GGCTTCGCTGGACGTAAACC+CGG 0.542853 2_3:-57170125 None:intergenic
GTGGTGTTTGGTGTGGAACT+AGG 0.543791 2_3:-57169926 None:intergenic
GCTTGGAATTTAACAATTTG+GGG 0.544684 2_3:+57170332 Msa0301290:CDS
ATTAGTAGTATCAATGGTGG+AGG 0.545112 2_3:-57169891 None:intergenic
AGAATTAGTAGTATCAATGG+TGG 0.550623 2_3:-57169894 None:intergenic
TGAAGAAGTGGTGTTTGGTG+TGG 0.550717 2_3:-57169933 None:intergenic
GCCACTGATTAGTGGTTGGA+GGG 0.566821 2_3:-57169373 None:intergenic
TACAGACAACAAAGAGCAGT+TGG 0.576785 2_3:-57169285 None:intergenic
TGCAAACGTTATTGGACAAG+AGG 0.578125 2_3:+57169779 Msa0301290:CDS
ACTCTTAGGAATGTTCCTGT+TGG 0.582456 2_3:+57169806 Msa0301290:CDS
AACCACTAATCAGTGGCCAC+AGG 0.599050 2_3:+57169379 Msa0301290:CDS
TTCCAGTAAAGTGAAGAAGC+TGG 0.599918 2_3:-57170424 None:intergenic
TGAAGGAGAGATGGAACATG+TGG 0.611078 2_3:+57170384 Msa0301290:CDS
TTTCAAATATAGGAGAGTGA+GGG 0.620308 2_3:-57169246 None:intergenic
GTTTCAAATATAGGAGAGTG+AGG 0.621310 2_3:-57169247 None:intergenic
AGAGCAGAACTCAACAGAGA+AGG 0.622062 2_3:-57170265 None:intergenic
TTCAAATATAGGAGAGTGAG+GGG 0.622411 2_3:-57169245 None:intergenic
AATCCAATGGTATTCATCTG+AGG 0.625381 2_3:-57170091 None:intergenic
GGATGAAAAGCCTAATCCAA+TGG 0.636487 2_3:-57170104 None:intergenic
AAACGTTATTGGACAAGAGG+TGG 0.639210 2_3:+57169782 Msa0301290:CDS
CAGTAAAGTGAAGAAGCTGG+TGG 0.648703 2_3:-57170421 None:intergenic
TCCCTCCAACCACTAATCAG+TGG 0.668953 2_3:+57169372 Msa0301290:CDS
TAATAAGTAAAGCTAAACAA+AGG 0.672843 2_3:-57170581 None:intergenic
TAAAACTAACAATCTAACTG+AGG 0.677835 2_3:-57170812 None:intergenic
GAAGGAGAGATGGAACATGT+GGG 0.678274 2_3:+57170385 Msa0301290:CDS
TTAGTAGTATCAATGGTGGA+GGG 0.679047 2_3:-57169890 None:intergenic
TGTGGCCACTGATTAGTGGT+TGG 0.681850 2_3:-57169377 None:intergenic
GGAGAGATGGAACATGTGGG+TGG 0.710445 2_3:+57170388 Msa0301290:CDS
GTGTTTGATGAGTCACAACG+TGG 0.749573 2_3:-57169698 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATAATTGATATAAGAAAA+AGG - chr2_3:57169571-57169590 None:intergenic 10.0%
!! ATATAATTGATATAAGAAAA+AGG - chr2_3:57169571-57169590 None:intergenic 10.0%
!!! TTTGTTTTGTTTTGTATTTT+TGG + chr2_3:57170640-57170659 Msa0301290:three_prime_UTR 15.0%
!!! TTGTTTTGTTTTGTATTTTT+GGG + chr2_3:57170641-57170660 Msa0301290:three_prime_UTR 15.0%
!! TTATTGAAAAAAGAAAAAGA+AGG + chr2_3:57170686-57170705 Msa0301290:three_prime_UTR 15.0%
!!! TTTGTTTTGTTTTGTATTTT+TGG + chr2_3:57170640-57170659 Msa0301290:three_prime_UTR 15.0%
!!! TTGTTTTGTTTTGTATTTTT+GGG + chr2_3:57170641-57170660 Msa0301290:three_prime_UTR 15.0%
!! TTATTGAAAAAAGAAAAAGA+AGG + chr2_3:57170686-57170705 Msa0301290:three_prime_UTR 15.0%
!! AATAGATGATGTTAAGATAA+TGG + chr2_3:57169625-57169644 Msa0301290:CDS 20.0%
!! AGGACTAATATTATGATTTA+AGG - chr2_3:57169966-57169985 None:intergenic 20.0%
!!! AATATTAGTCCTATGTTTTA+TGG + chr2_3:57169974-57169993 Msa0301290:CDS 20.0%
!!! GTTTAAGGTTATCTTTAATT+TGG + chr2_3:57170556-57170575 Msa0301290:three_prime_UTR 20.0%
!! TAATAAGTAAAGCTAAACAA+AGG - chr2_3:57170584-57170603 None:intergenic 20.0%
!! AAAAGAAGGAAGAAAAAAAA+AGG + chr2_3:57170700-57170719 Msa0301290:three_prime_UTR 20.0%
!! AATAGATGATGTTAAGATAA+TGG + chr2_3:57169625-57169644 Msa0301290:CDS 20.0%
!! AGGACTAATATTATGATTTA+AGG - chr2_3:57169966-57169985 None:intergenic 20.0%
!!! AATATTAGTCCTATGTTTTA+TGG + chr2_3:57169974-57169993 Msa0301290:CDS 20.0%
!!! GTTTAAGGTTATCTTTAATT+TGG + chr2_3:57170556-57170575 Msa0301290:three_prime_UTR 20.0%
!! TAATAAGTAAAGCTAAACAA+AGG - chr2_3:57170584-57170603 None:intergenic 20.0%
!! AAAAGAAGGAAGAAAAAAAA+AGG + chr2_3:57170700-57170719 Msa0301290:three_prime_UTR 20.0%
! TTTGTGTAAATAATTGTGCA+TGG - chr2_3:57169430-57169449 None:intergenic 25.0%
! AGCTAAGCTACAAAATTAAT+TGG - chr2_3:57169466-57169485 None:intergenic 25.0%
!!! TAATTTTGTAGCTTAGCTTT+TGG + chr2_3:57169468-57169487 Msa0301290:intron 25.0%
!! AGTTTAATAAGTTCTAGCTA+GGG - chr2_3:57169537-57169556 None:intergenic 25.0%
!! TAGTTTAATAAGTTCTAGCT+AGG - chr2_3:57169538-57169557 None:intergenic 25.0%
!!! GTTAAGATAATGGAAAAACA+AGG + chr2_3:57169635-57169654 Msa0301290:CDS 25.0%
! AGAAGAATTAGTAGTATCAA+TGG - chr2_3:57169900-57169919 None:intergenic 25.0%
! CTTGATAAACCATAAAACAT+AGG - chr2_3:57169986-57170005 None:intergenic 25.0%
! TGTATAACCATTATGATCAT+TGG - chr2_3:57170149-57170168 None:intergenic 25.0%
! GATCATAATGGTTATACAAA+TGG + chr2_3:57170151-57170170 Msa0301290:CDS 25.0%
! CAACATAAATTCATGAATGA+TGG + chr2_3:57170292-57170311 Msa0301290:CDS 25.0%
!! AATGATCAAGGTTCTAATAT+TGG + chr2_3:57170478-57170497 Msa0301290:CDS 25.0%
! ATAACCTTAAACTTGAACTA+AGG - chr2_3:57170548-57170567 None:intergenic 25.0%
!!! TTTGTTTTGTATTTTTGGGT+TGG + chr2_3:57170645-57170664 Msa0301290:three_prime_UTR 25.0%
!!! TTGTTTTGTATTTTTGGGTT+GGG + chr2_3:57170646-57170665 Msa0301290:three_prime_UTR 25.0%
!! AAGGATACTTGTTAGAAAAA+TGG + chr2_3:57170719-57170738 Msa0301290:three_prime_UTR 25.0%
!! AGGATACTTGTTAGAAAAAT+GGG + chr2_3:57170720-57170739 Msa0301290:three_prime_UTR 25.0%
! TAAAACTAACAATCTAACTG+AGG - chr2_3:57170815-57170834 None:intergenic 25.0%
! TTTGTGTAAATAATTGTGCA+TGG - chr2_3:57169430-57169449 None:intergenic 25.0%
! AGCTAAGCTACAAAATTAAT+TGG - chr2_3:57169466-57169485 None:intergenic 25.0%
!!! TAATTTTGTAGCTTAGCTTT+TGG + chr2_3:57169468-57169487 Msa0301290:intron 25.0%
!! AGTTTAATAAGTTCTAGCTA+GGG - chr2_3:57169537-57169556 None:intergenic 25.0%
!! TAGTTTAATAAGTTCTAGCT+AGG - chr2_3:57169538-57169557 None:intergenic 25.0%
!!! GTTAAGATAATGGAAAAACA+AGG + chr2_3:57169635-57169654 Msa0301290:CDS 25.0%
! AGAAGAATTAGTAGTATCAA+TGG - chr2_3:57169900-57169919 None:intergenic 25.0%
! CTTGATAAACCATAAAACAT+AGG - chr2_3:57169986-57170005 None:intergenic 25.0%
! TGTATAACCATTATGATCAT+TGG - chr2_3:57170149-57170168 None:intergenic 25.0%
! GATCATAATGGTTATACAAA+TGG + chr2_3:57170151-57170170 Msa0301290:CDS 25.0%
! CAACATAAATTCATGAATGA+TGG + chr2_3:57170292-57170311 Msa0301290:CDS 25.0%
!! AATGATCAAGGTTCTAATAT+TGG + chr2_3:57170478-57170497 Msa0301290:CDS 25.0%
! ATAACCTTAAACTTGAACTA+AGG - chr2_3:57170548-57170567 None:intergenic 25.0%
!!! TTTGTTTTGTATTTTTGGGT+TGG + chr2_3:57170645-57170664 Msa0301290:three_prime_UTR 25.0%
!!! TTGTTTTGTATTTTTGGGTT+GGG + chr2_3:57170646-57170665 Msa0301290:three_prime_UTR 25.0%
!! AAGGATACTTGTTAGAAAAA+TGG + chr2_3:57170719-57170738 Msa0301290:three_prime_UTR 25.0%
!! AGGATACTTGTTAGAAAAAT+GGG + chr2_3:57170720-57170739 Msa0301290:three_prime_UTR 25.0%
! TAAAACTAACAATCTAACTG+AGG - chr2_3:57170815-57170834 None:intergenic 25.0%
! ACTCTTCAAGGATTCTATTT+TGG + chr2_3:57169309-57169328 Msa0301290:five_prime_UTR 30.0%
AGAATTAGTAGTATCAATGG+TGG - chr2_3:57169897-57169916 None:intergenic 30.0%
TAAGGTTGAATTTGAAGAAG+TGG - chr2_3:57169948-57169967 None:intergenic 30.0%
GGCTTGGAATTTAACAATTT+GGG + chr2_3:57170331-57170350 Msa0301290:CDS 30.0%
GCTTGGAATTTAACAATTTG+GGG + chr2_3:57170332-57170351 Msa0301290:CDS 30.0%
GAATTTAACAATTTGGGGAA+TGG + chr2_3:57170337-57170356 Msa0301290:CDS 30.0%
AAAGGAATGAAGAAAGATGA+AGG + chr2_3:57170367-57170386 Msa0301290:CDS 30.0%
! CAATCCTTAGTTCAAGTTTA+AGG + chr2_3:57170541-57170560 Msa0301290:three_prime_UTR 30.0%
! ACTCTTCAAGGATTCTATTT+TGG + chr2_3:57169309-57169328 Msa0301290:five_prime_UTR 30.0%
AGAATTAGTAGTATCAATGG+TGG - chr2_3:57169897-57169916 None:intergenic 30.0%
TAAGGTTGAATTTGAAGAAG+TGG - chr2_3:57169948-57169967 None:intergenic 30.0%
GGCTTGGAATTTAACAATTT+GGG + chr2_3:57170331-57170350 Msa0301290:CDS 30.0%
GCTTGGAATTTAACAATTTG+GGG + chr2_3:57170332-57170351 Msa0301290:CDS 30.0%
GAATTTAACAATTTGGGGAA+TGG + chr2_3:57170337-57170356 Msa0301290:CDS 30.0%
AAAGGAATGAAGAAAGATGA+AGG + chr2_3:57170367-57170386 Msa0301290:CDS 30.0%
! CAATCCTTAGTTCAAGTTTA+AGG + chr2_3:57170541-57170560 Msa0301290:three_prime_UTR 30.0%
GTTTCAAATATAGGAGAGTG+AGG - chr2_3:57169250-57169269 None:intergenic 35.0%
GCTTCAAGGGTTTCAAATAT+AGG - chr2_3:57169259-57169278 None:intergenic 35.0%
TGTTGTCTGTATACTCTTCA+AGG + chr2_3:57169297-57169316 Msa0301290:five_prime_UTR 35.0%
! TTAGTAGTATCAATGGTGGA+GGG - chr2_3:57169893-57169912 None:intergenic 35.0%
ATTAGTAGTATCAATGGTGG+AGG - chr2_3:57169894-57169913 None:intergenic 35.0%
GAATTTGAAGAAGTGGTGTT+TGG - chr2_3:57169941-57169960 None:intergenic 35.0%
ATGTGAATCTTCCATTCTCA+AGG + chr2_3:57170023-57170042 Msa0301290:CDS 35.0%
GAGATATTGAACCTTGAGAA+TGG - chr2_3:57170037-57170056 None:intergenic 35.0%
! TCTAGAATCTCAACAAGTTC+AGG + chr2_3:57170055-57170074 Msa0301290:CDS 35.0%
AATCCAATGGTATTCATCTG+AGG - chr2_3:57170094-57170113 None:intergenic 35.0%
CAGATGAATACCATTGGATT+AGG + chr2_3:57170094-57170113 Msa0301290:CDS 35.0%
!! ATTGGATTAGGCTTTTCATC+CGG + chr2_3:57170106-57170125 Msa0301290:CDS 35.0%
GGGCTTGGAATTTAACAATT+TGG + chr2_3:57170330-57170349 Msa0301290:CDS 35.0%
! ATTGGTGTTTGGAATGATCA+AGG + chr2_3:57170466-57170485 Msa0301290:CDS 35.0%
! ACTTGTTAGAAAAATGGGAG+AGG + chr2_3:57170725-57170744 Msa0301290:three_prime_UTR 35.0%
TAGAAAAATGGGAGAGGTAA+AGG + chr2_3:57170731-57170750 Msa0301290:three_prime_UTR 35.0%
GTTTCAAATATAGGAGAGTG+AGG - chr2_3:57169250-57169269 None:intergenic 35.0%
GCTTCAAGGGTTTCAAATAT+AGG - chr2_3:57169259-57169278 None:intergenic 35.0%
TGTTGTCTGTATACTCTTCA+AGG + chr2_3:57169297-57169316 Msa0301290:five_prime_UTR 35.0%
! TTAGTAGTATCAATGGTGGA+GGG - chr2_3:57169893-57169912 None:intergenic 35.0%
ATTAGTAGTATCAATGGTGG+AGG - chr2_3:57169894-57169913 None:intergenic 35.0%
GAATTTGAAGAAGTGGTGTT+TGG - chr2_3:57169941-57169960 None:intergenic 35.0%
ATGTGAATCTTCCATTCTCA+AGG + chr2_3:57170023-57170042 Msa0301290:CDS 35.0%
GAGATATTGAACCTTGAGAA+TGG - chr2_3:57170037-57170056 None:intergenic 35.0%
! TCTAGAATCTCAACAAGTTC+AGG + chr2_3:57170055-57170074 Msa0301290:CDS 35.0%
AATCCAATGGTATTCATCTG+AGG - chr2_3:57170094-57170113 None:intergenic 35.0%
CAGATGAATACCATTGGATT+AGG + chr2_3:57170094-57170113 Msa0301290:CDS 35.0%
!! ATTGGATTAGGCTTTTCATC+CGG + chr2_3:57170106-57170125 Msa0301290:CDS 35.0%
GGGCTTGGAATTTAACAATT+TGG + chr2_3:57170330-57170349 Msa0301290:CDS 35.0%
! ATTGGTGTTTGGAATGATCA+AGG + chr2_3:57170466-57170485 Msa0301290:CDS 35.0%
! ACTTGTTAGAAAAATGGGAG+AGG + chr2_3:57170725-57170744 Msa0301290:three_prime_UTR 35.0%
TAGAAAAATGGGAGAGGTAA+AGG + chr2_3:57170731-57170750 Msa0301290:three_prime_UTR 35.0%
TACAGACAACAAAGAGCAGT+TGG - chr2_3:57169288-57169307 None:intergenic 40.0%
CAAGCTTTGCAGAAATGTCT+TGG - chr2_3:57169761-57169780 None:intergenic 40.0%
GCAAAGCTTGCAAACGTTAT+TGG + chr2_3:57169771-57169790 Msa0301290:CDS 40.0%
TGCAAACGTTATTGGACAAG+AGG + chr2_3:57169779-57169798 Msa0301290:CDS 40.0%
AAACGTTATTGGACAAGAGG+TGG + chr2_3:57169782-57169801 Msa0301290:CDS 40.0%
ACTCTTAGGAATGTTCCTGT+TGG + chr2_3:57169806-57169825 Msa0301290:CDS 40.0%
GGATGAAAAGCCTAATCCAA+TGG - chr2_3:57170107-57170126 None:intergenic 40.0%
!! TTGGATTAGGCTTTTCATCC+GGG + chr2_3:57170107-57170126 Msa0301290:CDS 40.0%
!! ATTATGATCATTGGCTTCGC+TGG - chr2_3:57170140-57170159 None:intergenic 40.0%
AGCGAAGCCAATGATCATAA+TGG + chr2_3:57170139-57170158 Msa0301290:CDS 40.0%
GGCATAACTGATGCATTACT+TGG - chr2_3:57170247-57170266 None:intergenic 40.0%
! TGATGGTAGCAACACTTTTC+AGG + chr2_3:57170309-57170328 Msa0301290:CDS 40.0%
! GATGGTAGCAACACTTTTCA+GGG + chr2_3:57170310-57170329 Msa0301290:CDS 40.0%
GAAGAAAGATGAAGGAGAGA+TGG + chr2_3:57170375-57170394 Msa0301290:CDS 40.0%
TTCCAGTAAAGTGAAGAAGC+TGG - chr2_3:57170427-57170446 None:intergenic 40.0%
ACAGCATCATCATCAGCTAT+TGG + chr2_3:57170448-57170467 Msa0301290:CDS 40.0%
! CATCATCAGCTATTGGTGTT+TGG + chr2_3:57170455-57170474 Msa0301290:CDS 40.0%
ACAATCTAACTGAGGTTTGC+TGG - chr2_3:57170807-57170826 None:intergenic 40.0%
TACAGACAACAAAGAGCAGT+TGG - chr2_3:57169288-57169307 None:intergenic 40.0%
CAAGCTTTGCAGAAATGTCT+TGG - chr2_3:57169761-57169780 None:intergenic 40.0%
GCAAAGCTTGCAAACGTTAT+TGG + chr2_3:57169771-57169790 Msa0301290:CDS 40.0%
TGCAAACGTTATTGGACAAG+AGG + chr2_3:57169779-57169798 Msa0301290:CDS 40.0%
AAACGTTATTGGACAAGAGG+TGG + chr2_3:57169782-57169801 Msa0301290:CDS 40.0%
ACTCTTAGGAATGTTCCTGT+TGG + chr2_3:57169806-57169825 Msa0301290:CDS 40.0%
GGATGAAAAGCCTAATCCAA+TGG - chr2_3:57170107-57170126 None:intergenic 40.0%
!! TTGGATTAGGCTTTTCATCC+GGG + chr2_3:57170107-57170126 Msa0301290:CDS 40.0%
!! ATTATGATCATTGGCTTCGC+TGG - chr2_3:57170140-57170159 None:intergenic 40.0%
AGCGAAGCCAATGATCATAA+TGG + chr2_3:57170139-57170158 Msa0301290:CDS 40.0%
GGCATAACTGATGCATTACT+TGG - chr2_3:57170247-57170266 None:intergenic 40.0%
! TGATGGTAGCAACACTTTTC+AGG + chr2_3:57170309-57170328 Msa0301290:CDS 40.0%
! GATGGTAGCAACACTTTTCA+GGG + chr2_3:57170310-57170329 Msa0301290:CDS 40.0%
GAAGAAAGATGAAGGAGAGA+TGG + chr2_3:57170375-57170394 Msa0301290:CDS 40.0%
TTCCAGTAAAGTGAAGAAGC+TGG - chr2_3:57170427-57170446 None:intergenic 40.0%
ACAGCATCATCATCAGCTAT+TGG + chr2_3:57170448-57170467 Msa0301290:CDS 40.0%
! CATCATCAGCTATTGGTGTT+TGG + chr2_3:57170455-57170474 Msa0301290:CDS 40.0%
ACAATCTAACTGAGGTTTGC+TGG - chr2_3:57170807-57170826 None:intergenic 40.0%
! CAGTTGGTCACTAGCTTCAA+GGG - chr2_3:57169272-57169291 None:intergenic 45.0%
TGCATGGAGATAGAGAGAGA+GGG - chr2_3:57169414-57169433 None:intergenic 45.0%
GTGTTTGATGAGTCACAACG+TGG - chr2_3:57169701-57169720 None:intergenic 45.0%
CTTAGGAATGTTCCTGTTGG+TGG + chr2_3:57169809-57169828 Msa0301290:CDS 45.0%
! TGAAGAAGTGGTGTTTGGTG+TGG - chr2_3:57169936-57169955 None:intergenic 45.0%
CAGCCTCAGATGAATACCAT+TGG + chr2_3:57170088-57170107 Msa0301290:CDS 45.0%
AGAGCAGAACTCAACAGAGA+AGG - chr2_3:57170268-57170287 None:intergenic 45.0%
!! TAGCAACACTTTTCAGGGCT+TGG + chr2_3:57170315-57170334 Msa0301290:CDS 45.0%
TTGGGGAATGGAAGTGAGAA+AGG + chr2_3:57170349-57170368 Msa0301290:CDS 45.0%
TGAAGGAGAGATGGAACATG+TGG + chr2_3:57170384-57170403 Msa0301290:CDS 45.0%
GAAGGAGAGATGGAACATGT+GGG + chr2_3:57170385-57170404 Msa0301290:CDS 45.0%
CAGTAAAGTGAAGAAGCTGG+TGG - chr2_3:57170424-57170443 None:intergenic 45.0%
CACCAGCTTCTTCACTTTAC+TGG + chr2_3:57170422-57170441 Msa0301290:CDS 45.0%
!! TGAGCTAGAGGAGAGTACTT+TGG + chr2_3:57170754-57170773 Msa0301290:three_prime_UTR 45.0%
TTGCTGGATGCAGCTTACAT+AGG - chr2_3:57170791-57170810 None:intergenic 45.0%
! CAGTTGGTCACTAGCTTCAA+GGG - chr2_3:57169272-57169291 None:intergenic 45.0%
TGCATGGAGATAGAGAGAGA+GGG - chr2_3:57169414-57169433 None:intergenic 45.0%
GTGTTTGATGAGTCACAACG+TGG - chr2_3:57169701-57169720 None:intergenic 45.0%
CTTAGGAATGTTCCTGTTGG+TGG + chr2_3:57169809-57169828 Msa0301290:CDS 45.0%
! TGAAGAAGTGGTGTTTGGTG+TGG - chr2_3:57169936-57169955 None:intergenic 45.0%
CAGCCTCAGATGAATACCAT+TGG + chr2_3:57170088-57170107 Msa0301290:CDS 45.0%
AGAGCAGAACTCAACAGAGA+AGG - chr2_3:57170268-57170287 None:intergenic 45.0%
!! TAGCAACACTTTTCAGGGCT+TGG + chr2_3:57170315-57170334 Msa0301290:CDS 45.0%
TTGGGGAATGGAAGTGAGAA+AGG + chr2_3:57170349-57170368 Msa0301290:CDS 45.0%
TGAAGGAGAGATGGAACATG+TGG + chr2_3:57170384-57170403 Msa0301290:CDS 45.0%
GAAGGAGAGATGGAACATGT+GGG + chr2_3:57170385-57170404 Msa0301290:CDS 45.0%
CAGTAAAGTGAAGAAGCTGG+TGG - chr2_3:57170424-57170443 None:intergenic 45.0%
CACCAGCTTCTTCACTTTAC+TGG + chr2_3:57170422-57170441 Msa0301290:CDS 45.0%
!! TGAGCTAGAGGAGAGTACTT+TGG + chr2_3:57170754-57170773 Msa0301290:three_prime_UTR 45.0%
TTGCTGGATGCAGCTTACAT+AGG - chr2_3:57170791-57170810 None:intergenic 45.0%
! GCAGTTGGTCACTAGCTTCA+AGG - chr2_3:57169273-57169292 None:intergenic 50.0%
CTGATTAGTGGTTGGAGGGA+TGG - chr2_3:57169372-57169391 None:intergenic 50.0%
TCCCTCCAACCACTAATCAG+TGG + chr2_3:57169372-57169391 Msa0301290:CDS 50.0%
! GCCACTGATTAGTGGTTGGA+GGG - chr2_3:57169376-57169395 None:intergenic 50.0%
! TGTGGCCACTGATTAGTGGT+TGG - chr2_3:57169380-57169399 None:intergenic 50.0%
AACCACTAATCAGTGGCCAC+AGG + chr2_3:57169379-57169398 Msa0301290:CDS 50.0%
! AACCTGTGGCCACTGATTAG+TGG - chr2_3:57169384-57169403 None:intergenic 50.0%
GTGCATGGAGATAGAGAGAG+AGG - chr2_3:57169415-57169434 None:intergenic 50.0%
GGACAAGAGGTGGTACTCTT+AGG + chr2_3:57169792-57169811 Msa0301290:CDS 50.0%
! AGGAATGTTCCTGTTGGTGG+TGG + chr2_3:57169812-57169831 Msa0301290:CDS 50.0%
CTTCTGTAACCACCACCAAC+AGG - chr2_3:57169824-57169843 None:intergenic 50.0%
!! GTGGTGTTTGGTGTGGAACT+AGG - chr2_3:57169929-57169948 None:intergenic 50.0%
GAGAGGTAAAGGTGAGCTAG+AGG + chr2_3:57170742-57170761 Msa0301290:three_prime_UTR 50.0%
! GCAGTTGGTCACTAGCTTCA+AGG - chr2_3:57169273-57169292 None:intergenic 50.0%
CTGATTAGTGGTTGGAGGGA+TGG - chr2_3:57169372-57169391 None:intergenic 50.0%
TCCCTCCAACCACTAATCAG+TGG + chr2_3:57169372-57169391 Msa0301290:CDS 50.0%
! GCCACTGATTAGTGGTTGGA+GGG - chr2_3:57169376-57169395 None:intergenic 50.0%
! TGTGGCCACTGATTAGTGGT+TGG - chr2_3:57169380-57169399 None:intergenic 50.0%
AACCACTAATCAGTGGCCAC+AGG + chr2_3:57169379-57169398 Msa0301290:CDS 50.0%
! AACCTGTGGCCACTGATTAG+TGG - chr2_3:57169384-57169403 None:intergenic 50.0%
GTGCATGGAGATAGAGAGAG+AGG - chr2_3:57169415-57169434 None:intergenic 50.0%
GGACAAGAGGTGGTACTCTT+AGG + chr2_3:57169792-57169811 Msa0301290:CDS 50.0%
! AGGAATGTTCCTGTTGGTGG+TGG + chr2_3:57169812-57169831 Msa0301290:CDS 50.0%
CTTCTGTAACCACCACCAAC+AGG - chr2_3:57169824-57169843 None:intergenic 50.0%
!! GTGGTGTTTGGTGTGGAACT+AGG - chr2_3:57169929-57169948 None:intergenic 50.0%
GAGAGGTAAAGGTGAGCTAG+AGG + chr2_3:57170742-57170761 Msa0301290:three_prime_UTR 50.0%
! GGCCACTGATTAGTGGTTGG+AGG - chr2_3:57169377-57169396 None:intergenic 55.0%
GAGAGGGAGAGATGAACCTG+TGG - chr2_3:57169398-57169417 None:intergenic 55.0%
GGAGAGATGGAACATGTGGG+TGG + chr2_3:57170388-57170407 Msa0301290:CDS 55.0%
! GGCCACTGATTAGTGGTTGG+AGG - chr2_3:57169377-57169396 None:intergenic 55.0%
GAGAGGGAGAGATGAACCTG+TGG - chr2_3:57169398-57169417 None:intergenic 55.0%
GGAGAGATGGAACATGTGGG+TGG + chr2_3:57170388-57170407 Msa0301290:CDS 55.0%
GGCTTCGCTGGACGTAAACC+CGG - chr2_3:57170128-57170147 None:intergenic 60.0%
GGCTTCGCTGGACGTAAACC+CGG - chr2_3:57170128-57170147 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr2_3 gene 57169247 57170840 57169247 ID=Msa0301290;Name=Msa0301290
chr2_3 mRNA 57169247 57170840 57169247 ID=Msa0301290-mRNA-1;Parent=Msa0301290;Name=Msa0301290-mRNA-1;_AED=0.00;_eAED=0.00;_QI=94|1|1|1|1|1|2|315|321
chr2_3 exon 57169247 57169400 57169247 ID=Msa0301290-mRNA-1:exon:18376;Parent=Msa0301290-mRNA-1
chr2_3 exon 57169620 57170840 57169620 ID=Msa0301290-mRNA-1:exon:18377;Parent=Msa0301290-mRNA-1
chr2_3 five_prime_UTR 57169247 57169340 57169247 ID=Msa0301290-mRNA-1:five_prime_utr;Parent=Msa0301290-mRNA-1
chr2_3 CDS 57169341 57169400 57169341 ID=Msa0301290-mRNA-1:cds;Parent=Msa0301290-mRNA-1
chr2_3 CDS 57169620 57170525 57169620 ID=Msa0301290-mRNA-1:cds;Parent=Msa0301290-mRNA-1
chr2_3 three_prime_UTR 57170526 57170840 57170526 ID=Msa0301290-mRNA-1:three_prime_utr;Parent=Msa0301290-mRNA-1
Gene Sequence

>Msa0301290

ATGTTATCTAACTATGAGAAAATGCTTGCCATCCCTCCAACCACTAATCAGTGGCCACAGAATCAAATAGATGATGTTAAGATAATGGAAAAACAAGGTCAAGAACTTCAGCAGCAGCAGCAGCAAGCTCTCAAGTGTCCACGTTGTGACTCATCAAACACAAAGTTTTGTTACTACAACAACTACAGTTTGTCACAACCAAGACATTTCTGCAAAGCTTGCAAACGTTATTGGACAAGAGGTGGTACTCTTAGGAATGTTCCTGTTGGTGGTGGTTACAGAAGAAACAACTCAAACAAACGTAGTAACAATACTACTCTCATCAAAAGACCCTCCACCATTGATACTACTAATTCTTCTTCAACTCCTAGTTCCACACCAAACACCACTTCTTCAAATTCAACCTTAAATCATAATATTAGTCCTATGTTTTATGGTTTATCAAGTAGTAATAACTCTTGTGATGTGAATCTTCCATTCTCAAGGTTCAATATCTCTAGAATCTCAACAAGTTCAGGTTATGATTTTCAGCCTCAGATGAATACCATTGGATTAGGCTTTTCATCCGGGTTTACGTCCAGCGAAGCCAATGATCATAATGGTTATACAAATGGATTTACTTCAAATAATAATAATAACACTCTTGTCTCAAGATATGATTCAATCTTTAGCTCTTCTTCTGCACCAAGTAATGCATCAGTTATGCCTTCTCTGTTGAGTTCTGCTCTGCTGCAACATAAATTCATGAATGATGGTAGCAACACTTTTCAGGGCTTGGAATTTAACAATTTGGGGAATGGAAGTGAGAAAGGAATGAAGAAAGATGAAGGAGAGATGGAACATGTGGGTGGTTTGTATGATCCACCAGCTTCTTCACTTTACTGGAATACAGCATCATCATCAGCTATTGGTGTTTGGAATGATCAAGGTTCTAATATTGGTTCATCAGTTACTTCTTTGATCTAG
Protein sequence

>Msa0301290

MLSNYEKMLAIPPTTNQWPQNQIDDVKIMEKQGQELQQQQQQALKCPRCDSSNTKFCYYNNYSLSQPRHFCKACKRYWTRGGTLRNVPVGGGYRRNNSNKRSNNTTLIKRPSTIDTTNSSSTPSSTPNTTSSNSTLNHNISPMFYGLSSSNNSCDVNLPFSRFNISRISTSSGYDFQPQMNTIGLGFSSGFTSSEANDHNGYTNGFTSNNNNNTLVSRYDSIFSSSSAPSNASVMPSLLSSALLQHKFMNDGSNTFQGLEFNNLGNGSEKGMKKDEGEMEHVGGLYDPPASSLYWNTASSSAIGVWNDQGSNIGSSVTSLI*