Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0304360 | XP_003597853.1 | 85.897 | 78 | 6 | 1 | 1 | 73 | 1 | 78 | 2.19e-41 | 144 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0304360 | G7IUS5 | 85.897 | 78 | 6 | 1 | 1 | 73 | 1 | 78 | 1.05e-41 | 144 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0304360 | TF | C3H |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0304360 | MtrunA17_Chr2g0332631 | 85.897 | 78 | 6 | 1 | 1 | 73 | 1 | 78 | 1.71e-44 | 140 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTGAGTCTTAATCCTCATA+TGG | 0.366307 | 2_3:-61656056 | None:intergenic |
| GCCATGTGCATAGTTGCATT+GGG | 0.419796 | 2_3:-61656121 | None:intergenic |
| TGAACATAATGAAGAGGTAG+AGG | 0.427658 | 2_3:-61656249 | None:intergenic |
| AATTTGAAGCAATGCAACTA+TGG | 0.438057 | 2_3:-61655980 | None:intergenic |
| TTCACAAGAGTGGTTGAAGA+TGG | 0.445076 | 2_3:+61656008 | Msa0304360:exon |
| AACGACCTCAGAACAGCAAC+AGG | 0.451715 | 2_3:+61656147 | Msa0304360:CDS |
| AAAATTCACAAACCCATATG+AGG | 0.470113 | 2_3:+61656043 | Msa0304360:CDS |
| ATGAACATTCTACAGAATCT+TGG | 0.480970 | 2_3:-61656171 | None:intergenic |
| AGCCATGTGCATAGTTGCAT+TGG | 0.488645 | 2_3:-61656122 | None:intergenic |
| CTGAGTCTTAATCCTCATAT+GGG | 0.496970 | 2_3:-61656055 | None:intergenic |
| ACATAATGAAGAGGTAGAGG+AGG | 0.543147 | 2_3:-61656246 | None:intergenic |
| GTAGAGGAGGAGTTGCATGA+AGG | 0.548027 | 2_3:-61656233 | None:intergenic |
| AAATTCATCGTTCACAAGAG+TGG | 0.555414 | 2_3:+61655998 | Msa0304360:five_prime_UTR |
| TTGGTCCTGTTGCTGTTCTG+AGG | 0.555812 | 2_3:-61656152 | None:intergenic |
| CCCCAATGCAACTATGCACA+TGG | 0.558064 | 2_3:+61656120 | Msa0304360:CDS |
| CACACCAGACATTGTAGCTA+CGG | 0.572650 | 2_3:+61656195 | Msa0304360:intron |
| TTGCATTGGGGTGCAACAAG+AGG | 0.590171 | 2_3:-61656108 | None:intergenic |
| TGAGGATGAACATAATGAAG+AGG | 0.648497 | 2_3:-61656255 | None:intergenic |
| CCATGTGCATAGTTGCATTG+GGG | 0.656451 | 2_3:-61656120 | None:intergenic |
| CTATGCAAAAGATTCATGCA+AGG | 0.677580 | 2_3:+61656078 | Msa0304360:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AATTTGAAGCAATGCAACTA+TGG | - | chr2_3:61655983-61656002 | None:intergenic | 30.0% |
| AAAATTCACAAACCCATATG+AGG | + | chr2_3:61656043-61656062 | Msa0304360:CDS | 30.0% | |
| ATGAACATTCTACAGAATCT+TGG | - | chr2_3:61656174-61656193 | None:intergenic | 30.0% | |
| AAATTCATCGTTCACAAGAG+TGG | + | chr2_3:61655998-61656017 | Msa0304360:five_prime_UTR | 35.0% | |
| CTGAGTCTTAATCCTCATAT+GGG | - | chr2_3:61656058-61656077 | None:intergenic | 35.0% | |
| CTATGCAAAAGATTCATGCA+AGG | + | chr2_3:61656078-61656097 | Msa0304360:CDS | 35.0% | |
| TGAACATAATGAAGAGGTAG+AGG | - | chr2_3:61656252-61656271 | None:intergenic | 35.0% | |
| TGAGGATGAACATAATGAAG+AGG | - | chr2_3:61656258-61656277 | None:intergenic | 35.0% | |
| TTCACAAGAGTGGTTGAAGA+TGG | + | chr2_3:61656008-61656027 | Msa0304360:exon | 40.0% | |
| GCTGAGTCTTAATCCTCATA+TGG | - | chr2_3:61656059-61656078 | None:intergenic | 40.0% | |
| ACATAATGAAGAGGTAGAGG+AGG | - | chr2_3:61656249-61656268 | None:intergenic | 40.0% | |
| CCATGTGCATAGTTGCATTG+GGG | - | chr2_3:61656123-61656142 | None:intergenic | 45.0% | |
| GCCATGTGCATAGTTGCATT+GGG | - | chr2_3:61656124-61656143 | None:intergenic | 45.0% | |
| AGCCATGTGCATAGTTGCAT+TGG | - | chr2_3:61656125-61656144 | None:intergenic | 45.0% | |
| CACACCAGACATTGTAGCTA+CGG | + | chr2_3:61656195-61656214 | Msa0304360:intron | 45.0% | |
| TTGCATTGGGGTGCAACAAG+AGG | - | chr2_3:61656111-61656130 | None:intergenic | 50.0% | |
| CCCCAATGCAACTATGCACA+TGG | + | chr2_3:61656120-61656139 | Msa0304360:CDS | 50.0% | |
| AACGACCTCAGAACAGCAAC+AGG | + | chr2_3:61656147-61656166 | Msa0304360:CDS | 50.0% | |
| ! | TTGGTCCTGTTGCTGTTCTG+AGG | - | chr2_3:61656155-61656174 | None:intergenic | 50.0% |
| GTGTCCGTAGCTACAATGTC+TGG | - | chr2_3:61656202-61656221 | None:intergenic | 50.0% | |
| GTAGAGGAGGAGTTGCATGA+AGG | - | chr2_3:61656236-61656255 | None:intergenic | 50.0% | |
| TCATGCTGCTGCTGCTGCTG+AGG | - | chr2_3:61656276-61656295 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_3 | gene | 61655978 | 61656311 | 61655978 | ID=Msa0304360;Name=Msa0304360 |
| chr2_3 | mRNA | 61655978 | 61656311 | 61655978 | ID=Msa0304360-mRNA-1;Parent=Msa0304360;Name=Msa0304360-mRNA-1;_AED=0.25;_eAED=0.25;_QI=49|0|0|1|0|0|2|0|90 |
| chr2_3 | exon | 61655978 | 61656187 | 61655978 | ID=Msa0304360-mRNA-1:exon:20489;Parent=Msa0304360-mRNA-1 |
| chr2_3 | exon | 61656203 | 61656311 | 61656203 | ID=Msa0304360-mRNA-1:exon:20490;Parent=Msa0304360-mRNA-1 |
| chr2_3 | five_prime_UTR | 61655978 | 61656026 | 61655978 | ID=Msa0304360-mRNA-1:five_prime_utr;Parent=Msa0304360-mRNA-1 |
| chr2_3 | CDS | 61656027 | 61656187 | 61656027 | ID=Msa0304360-mRNA-1:cds;Parent=Msa0304360-mRNA-1 |
| chr2_3 | CDS | 61656203 | 61656311 | 61656203 | ID=Msa0304360-mRNA-1:cds;Parent=Msa0304360-mRNA-1 |
| Gene Sequence |
| Protein sequence |
