Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0307870 | XP_013450912.1 | 76.923 | 156 | 17 | 3 | 2 | 156 | 65 | 202 | 1.90e-73 | 229 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|---|---|
| Msa0307870 | TF | LOB |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0307870 | MtrunA17_Chr6g0452671 | 76.923 | 156 | 17 | 3 | 2 | 156 | 65 | 202 | 1.75e-77 | 229 |
| Msa0307870 | MtrunA17_Chr6g0452661 | 38.342 | 193 | 80 | 6 | 2 | 169 | 64 | 242 | 6.79e-26 | 99.4 |
| Msa0307870 | MtrunA17_Chr5g0439111 | 36.957 | 184 | 75 | 7 | 4 | 161 | 66 | 234 | 1.57e-21 | 87.8 |
| Msa0307870 | MtrunA17_Chr4g0055441 | 42.400 | 125 | 45 | 4 | 2 | 126 | 57 | 154 | 1.69e-19 | 80.5 |
| Msa0307870 | MtrunA17_Chr6g0452691 | 45.918 | 98 | 43 | 2 | 3 | 97 | 65 | 155 | 6.37e-18 | 77.0 |
| Msa0307870 | MtrunA17_Chr6g0449311 | 51.685 | 89 | 38 | 2 | 2 | 88 | 28 | 113 | 4.67e-14 | 65.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 27 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTCCATTGTGAAGAATTAT+TGG | 0.152750 | 2_4:+1070741 | Msa0307870:CDS |
| AAACTTTGAAACTAATTCTA+TGG | 0.254760 | 2_4:+1070639 | Msa0307870:CDS |
| CAAAATTTGTACTACCATTA+AGG | 0.279065 | 2_4:-1070765 | None:intergenic |
| GAAGAATTATTGGACCTTAA+TGG | 0.283903 | 2_4:+1070751 | Msa0307870:CDS |
| GATGTTATTATGATGTATTA+TGG | 0.406805 | 2_4:+1070412 | Msa0307870:CDS |
| ACATCAAGACATCCACCAAC+AGG | 0.415425 | 2_4:-1070394 | None:intergenic |
| ATTCTTACTAGATCATTGTA+AGG | 0.440710 | 2_4:+1070474 | Msa0307870:CDS |
| GGGATGTCATAATAAGAATT+CGG | 0.449186 | 2_4:-1070580 | None:intergenic |
| ACAATGATCAATTGATAAGT+AGG | 0.452504 | 2_4:-1070530 | None:intergenic |
| GGAACTCGAGTGCTACTAGA+AGG | 0.478886 | 2_4:-1070559 | None:intergenic |
| TCCTTGTGTTACCATCAAGT+TGG | 0.488291 | 2_4:-1070716 | None:intergenic |
| CGTGCTCAATTCCCTGTTGG+TGG | 0.544713 | 2_4:+1070382 | Msa0307870:CDS |
| GTTATACAATTGATCGGTGG+TGG | 0.566006 | 2_4:+1070673 | Msa0307870:CDS |
| CTAATTCTATGGATGCAACA+AGG | 0.571579 | 2_4:+1070650 | Msa0307870:CDS |
| GGTCCAATAATTCTTCACAA+TGG | 0.586783 | 2_4:-1070744 | None:intergenic |
| CAATGATCAATTGATAAGTA+GGG | 0.590328 | 2_4:-1070529 | None:intergenic |
| ACAAGGGTTATACAATTGAT+CGG | 0.617732 | 2_4:+1070667 | Msa0307870:CDS |
| TAATTCTATGGATGCAACAA+GGG | 0.625530 | 2_4:+1070651 | Msa0307870:CDS |
| ATGCGTGCTCAATTCCCTGT+TGG | 0.627603 | 2_4:+1070379 | Msa0307870:CDS |
| GATCGGTGGTGGCAATGCAA+TGG | 0.634656 | 2_4:+1070684 | Msa0307870:CDS |
| TCGAGTGCTACTAGAAGGAG+CGG | 0.637892 | 2_4:-1070554 | None:intergenic |
| GGGTTAATAGTAACATTACG+AGG | 0.645055 | 2_4:-1070601 | None:intergenic |
| CATCAAGACATCCACCAACA+GGG | 0.653135 | 2_4:-1070393 | None:intergenic |
| TGCTGATAAAGTAGTAACTG+AGG | 0.661688 | 2_4:+1070816 | Msa0307870:CDS |
| GGTTAATAGTAACATTACGA+GGG | 0.671173 | 2_4:-1070600 | None:intergenic |
| TCCAACTTGATGGTAACACA+AGG | 0.676070 | 2_4:+1070715 | Msa0307870:CDS |
| AGGGTTATACAATTGATCGG+TGG | 0.726517 | 2_4:+1070670 | Msa0307870:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTAATTATTATTATTTTT+TGG | + | chr2_4:1070858-1070877 | Msa0307870:intron | 0.0% |
| !!! | TTTAATTATTATTATTTTTT+GGG | + | chr2_4:1070859-1070878 | Msa0307870:intron | 0.0% |
| !!! | TAGTACAAATTTTGATTTAA+GGG | + | chr2_4:1070774-1070793 | Msa0307870:CDS | 15.0% |
| !! | TAAAGATATGTAATTAATGA+AGG | - | chr2_4:1070977-1070996 | None:intergenic | 15.0% |
| !! | TACATATCTTTAATCAATAT+AGG | + | chr2_4:1070985-1071004 | Msa0307870:intron | 15.0% |
| !! | GATGTTATTATGATGTATTA+TGG | + | chr2_4:1070412-1070431 | Msa0307870:CDS | 20.0% |
| !!! | ATTAGTTTCAAAGTTTTGTT+TGG | - | chr2_4:1070635-1070654 | None:intergenic | 20.0% |
| !!! | AAACTTTGAAACTAATTCTA+TGG | + | chr2_4:1070639-1070658 | Msa0307870:CDS | 20.0% |
| !!! | GTAGTACAAATTTTGATTTA+AGG | + | chr2_4:1070773-1070792 | Msa0307870:CDS | 20.0% |
| ! | ATTCTTACTAGATCATTGTA+AGG | + | chr2_4:1070474-1070493 | Msa0307870:CDS | 25.0% |
| ! | CAATGATCAATTGATAAGTA+GGG | - | chr2_4:1070532-1070551 | None:intergenic | 25.0% |
| ! | ACAATGATCAATTGATAAGT+AGG | - | chr2_4:1070533-1070552 | None:intergenic | 25.0% |
| !!! | AGTTTTGTTTGGTATCAATT+GGG | - | chr2_4:1070624-1070643 | None:intergenic | 25.0% |
| !!! | AAGTTTTGTTTGGTATCAAT+TGG | - | chr2_4:1070625-1070644 | None:intergenic | 25.0% |
| ! | ATTCCATTGTGAAGAATTAT+TGG | + | chr2_4:1070741-1070760 | Msa0307870:CDS | 25.0% |
| ! | CAAAATTTGTACTACCATTA+AGG | - | chr2_4:1070768-1070787 | None:intergenic | 25.0% |
| !!! | AAATTTTGATTTAAGGGAAG+AGG | + | chr2_4:1070780-1070799 | Msa0307870:CDS | 25.0% |
| !!! | CAAGATTTTGTTGTTGCAAA+GGG | - | chr2_4:1070507-1070526 | None:intergenic | 30.0% |
| !!! | TCAAGATTTTGTTGTTGCAA+AGG | - | chr2_4:1070508-1070527 | None:intergenic | 30.0% |
| GGGATGTCATAATAAGAATT+CGG | - | chr2_4:1070583-1070602 | None:intergenic | 30.0% | |
| GGTTAATAGTAACATTACGA+GGG | - | chr2_4:1070603-1070622 | None:intergenic | 30.0% | |
| TAATTCTATGGATGCAACAA+GGG | + | chr2_4:1070651-1070670 | Msa0307870:CDS | 30.0% | |
| ACAAGGGTTATACAATTGAT+CGG | + | chr2_4:1070667-1070686 | Msa0307870:CDS | 30.0% | |
| GAAGAATTATTGGACCTTAA+TGG | + | chr2_4:1070751-1070770 | Msa0307870:CDS | 30.0% | |
| GGGTTAATAGTAACATTACG+AGG | - | chr2_4:1070604-1070623 | None:intergenic | 35.0% | |
| CTAATTCTATGGATGCAACA+AGG | + | chr2_4:1070650-1070669 | Msa0307870:CDS | 35.0% | |
| GGAAGAAAAATCCAACTTGA+TGG | + | chr2_4:1070705-1070724 | Msa0307870:CDS | 35.0% | |
| GGTCCAATAATTCTTCACAA+TGG | - | chr2_4:1070747-1070766 | None:intergenic | 35.0% | |
| TGCTGATAAAGTAGTAACTG+AGG | + | chr2_4:1070816-1070835 | Msa0307870:CDS | 35.0% | |
| AGGGTTATACAATTGATCGG+TGG | + | chr2_4:1070670-1070689 | Msa0307870:CDS | 40.0% | |
| GTTATACAATTGATCGGTGG+TGG | + | chr2_4:1070673-1070692 | Msa0307870:CDS | 40.0% | |
| ! | TCCAACTTGATGGTAACACA+AGG | + | chr2_4:1070715-1070734 | Msa0307870:CDS | 40.0% |
| TCCTTGTGTTACCATCAAGT+TGG | - | chr2_4:1070719-1070738 | None:intergenic | 40.0% | |
| CATCAAGACATCCACCAACA+GGG | - | chr2_4:1070396-1070415 | None:intergenic | 45.0% | |
| ACATCAAGACATCCACCAAC+AGG | - | chr2_4:1070397-1070416 | None:intergenic | 45.0% | |
| ATGCGTGCTCAATTCCCTGT+TGG | + | chr2_4:1070379-1070398 | Msa0307870:CDS | 50.0% | |
| ! | TCGAGTGCTACTAGAAGGAG+CGG | - | chr2_4:1070557-1070576 | None:intergenic | 50.0% |
| ! | GGAACTCGAGTGCTACTAGA+AGG | - | chr2_4:1070562-1070581 | None:intergenic | 50.0% |
| CGTGCTCAATTCCCTGTTGG+TGG | + | chr2_4:1070382-1070401 | Msa0307870:CDS | 55.0% | |
| ! | GATCGGTGGTGGCAATGCAA+TGG | + | chr2_4:1070684-1070703 | Msa0307870:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_4 | gene | 1070340 | 1071045 | 1070340 | ID=Msa0307870;Name=Msa0307870 |
| chr2_4 | mRNA | 1070340 | 1071045 | 1070340 | ID=Msa0307870-mRNA-1;Parent=Msa0307870;Name=Msa0307870-mRNA-1;_AED=0.19;_eAED=0.19;_QI=0|0|0|0.5|1|1|2|0|178 |
| chr2_4 | exon | 1070340 | 1070837 | 1070340 | ID=Msa0307870-mRNA-1:exon:394;Parent=Msa0307870-mRNA-1 |
| chr2_4 | exon | 1071007 | 1071045 | 1071007 | ID=Msa0307870-mRNA-1:exon:395;Parent=Msa0307870-mRNA-1 |
| chr2_4 | CDS | 1070340 | 1070837 | 1070340 | ID=Msa0307870-mRNA-1:cds;Parent=Msa0307870-mRNA-1 |
| chr2_4 | CDS | 1071007 | 1071045 | 1071007 | ID=Msa0307870-mRNA-1:cds;Parent=Msa0307870-mRNA-1 |
| Gene Sequence |
| Protein sequence |
