AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0313090

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0313090 MtrunA17_Chr2g0282511 89.538 325 18 4 1 325 1 309 6.87e-165 461
Msa0313090 MtrunA17_Chr4g0047001 43.909 394 130 19 6 325 8 384 1.34e-60 198
Msa0313090 MtrunA17_Chr4g0031411 59.223 103 26 2 42 143 72 159 1.75e-32 123
Msa0313090 MtrunA17_Chr4g0010151 57.018 114 47 2 29 140 66 179 6.39e-32 122
Msa0313090 MtrunA17_Chr8g0374251 71.429 70 20 0 40 109 63 132 1.31e-31 121
Msa0313090 MtrunA17_Chr2g0328021 62.500 88 19 1 45 132 62 135 1.77e-31 119
Msa0313090 MtrunA17_Chr1g0177251 48.872 133 64 2 35 167 2 130 2.01e-31 119
Msa0313090 MtrunA17_Chr7g0223311 65.753 73 25 0 35 107 67 139 1.17e-30 119
Msa0313090 MtrunA17_Chr8g0374111 67.647 68 22 0 41 108 25 92 1.35e-30 115
Msa0313090 MtrunA17_Chr1g0188851 55.670 97 33 2 15 101 3 99 1.43e-30 117
Msa0313090 MtrunA17_Chr4g0030051 84.211 57 9 0 41 97 37 93 2.77e-30 116
Msa0313090 MtrunA17_Chr8g0367161 61.176 85 27 1 40 124 27 105 1.76e-29 115
Msa0313090 MtrunA17_Chr8g0341671 72.727 66 16 1 41 106 16 79 1.92e-29 112
Msa0313090 MtrunA17_Chr3g0127201 76.562 64 12 1 40 103 22 82 2.43e-29 114
Msa0313090 MtrunA17_Chr2g0282051 57.955 88 32 2 18 101 15 101 3.86e-29 112
Msa0313090 MtrunA17_Chr7g0237921 62.105 95 32 1 41 131 72 166 4.17e-29 114
Msa0313090 MtrunA17_Chr2g0326001 72.059 68 18 1 31 97 25 92 4.19e-29 113
Msa0313090 MtrunA17_Chr8g0346551 54.369 103 34 3 31 133 11 100 7.55e-29 112
Msa0313090 MtrunA17_Chr5g0411951 79.630 54 11 0 42 95 40 93 3.03e-28 112
Msa0313090 MtrunA17_Chr3g0126201 73.016 63 17 0 44 106 50 112 9.71e-28 110
Msa0313090 MtrunA17_Chr2g0308011 86.000 50 7 0 44 93 36 85 1.59e-27 107
Msa0313090 MtrunA17_Chr4g0061761 81.132 53 10 0 43 95 38 90 1.77e-27 109
Msa0313090 MtrunA17_Chr2g0282441 77.586 58 13 0 39 96 19 76 2.07e-27 109
Msa0313090 MtrunA17_Chr3g0117601 82.692 52 9 0 44 95 60 111 3.21e-27 108
Msa0313090 MtrunA17_Chr4g0047761 75.000 60 14 1 43 102 70 128 8.67e-27 107
Msa0313090 MtrunA17_Chr3g0092111 52.688 93 43 1 34 126 125 216 4.45e-26 107
Msa0313090 MtrunA17_Chr6g0453321 54.321 81 34 1 20 97 108 188 2.03e-25 106
Msa0313090 MtrunA17_Chr5g0417561 45.299 117 63 1 13 129 88 203 5.00e-25 103
Msa0313090 MtrunA17_Chr4g0042481 53.947 76 35 0 34 109 111 186 1.31e-24 103
Msa0313090 MtrunA17_Chr8g0355261 50.588 85 38 1 34 118 107 187 2.35e-24 102
Msa0313090 MtrunA17_Chr7g0217581 68.421 57 18 0 43 99 230 286 5.18e-24 102
Msa0313090 MtrunA17_Chr5g0417571 53.846 78 34 1 22 97 113 190 6.87e-24 99.8
Msa0313090 MtrunA17_Chr5g0417551 43.182 132 71 2 13 141 96 226 7.20e-24 100
Msa0313090 MtrunA17_Chr2g0283811 61.017 59 23 0 38 96 40 98 1.04e-23 95.5
Msa0313090 MtrunA17_Chr7g0254161 55.882 68 30 0 30 97 86 153 1.43e-23 100
Msa0313090 MtrunA17_Chr6g0460721 57.812 64 27 0 34 97 78 141 2.39e-23 98.2
Msa0313090 MtrunA17_Chr6g0460731 56.250 64 28 0 34 97 85 148 1.73e-22 96.7
Msa0313090 MtrunA17_Chr5g0417601 50.000 86 40 1 15 100 113 195 3.37e-22 95.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0313090 AT1G28310.1 43.182 308 125 13 45 325 27 311 5.48e-45 155
Msa0313090 AT1G28310.2 43.182 308 125 13 45 325 51 335 8.92e-45 156
Msa0313090 AT3G55370.1 53.774 106 48 1 4 109 36 140 1.46e-33 126
Msa0313090 AT3G55370.3 53.774 106 48 1 4 109 81 185 2.29e-33 126
Msa0313090 AT3G55370.2 53.774 106 48 1 4 109 36 140 2.50e-33 126
Msa0313090 AT1G51700.1 42.945 163 81 5 31 190 21 174 6.58e-32 118
Msa0313090 AT3G50410.1 65.333 75 26 0 29 103 14 88 2.47e-31 118
Msa0313090 AT3G45610.1 40.361 166 79 4 42 207 37 182 5.19e-31 117
Msa0313090 AT2G28810.2 81.034 58 11 0 39 96 71 128 9.41e-31 119
Msa0313090 AT1G07640.1 78.689 61 13 0 45 105 21 81 9.45e-31 117
Msa0313090 AT1G07640.2 78.689 61 13 0 45 105 77 137 1.29e-30 118
Msa0313090 AT2G28810.1 81.034 58 11 0 39 96 88 145 1.49e-30 118
Msa0313090 AT1G07640.3 78.689 61 13 0 45 105 85 145 1.70e-30 118
Msa0313090 AT5G65590.1 73.846 65 17 0 40 104 36 100 3.02e-30 117
Msa0313090 AT2G37590.1 65.753 73 25 0 29 101 73 145 3.54e-30 117
Msa0313090 AT5G60200.1 64.865 74 26 0 39 112 49 122 4.81e-30 115
Msa0313090 AT2G28510.1 39.891 183 81 6 43 217 46 207 1.38e-29 114
Msa0313090 AT5G02460.1 87.037 54 7 0 44 97 94 147 2.02e-29 116
Msa0313090 AT3G21270.1 78.947 57 12 0 45 101 29 85 4.05e-29 111
Msa0313090 AT5G60850.1 55.670 97 26 2 41 137 47 126 1.49e-28 112
Msa0313090 AT5G66940.1 46.154 130 64 4 27 153 13 139 2.48e-28 109
Msa0313090 AT4G38000.1 78.947 57 12 0 42 98 38 94 3.38e-28 110
Msa0313090 AT4G24060.1 48.571 105 30 2 15 97 3 105 5.43e-28 111
Msa0313090 AT1G21340.1 42.949 156 55 6 38 164 31 181 8.37e-28 109
Msa0313090 AT3G61850.2 66.667 63 21 0 35 97 52 114 2.71e-27 108
Msa0313090 AT3G61850.3 66.667 63 21 0 35 97 52 114 2.71e-27 108
Msa0313090 AT3G61850.5 65.217 69 21 1 35 103 52 117 2.94e-27 108
Msa0313090 AT3G61850.1 65.217 69 21 1 35 103 64 129 3.59e-27 108
Msa0313090 AT3G61850.4 65.217 69 21 1 35 103 64 129 4.49e-27 108
Msa0313090 AT3G52440.1 74.194 62 14 1 47 106 27 88 2.71e-26 105
Msa0313090 AT3G52440.2 74.194 62 14 1 47 106 46 107 3.68e-26 105
Msa0313090 AT4G00940.1 75.000 56 14 0 42 97 65 120 1.17e-25 104
Msa0313090 AT4G00940.3 75.000 56 14 0 42 97 65 120 1.17e-25 104
Msa0313090 AT4G00940.2 75.000 56 14 0 42 97 65 120 1.17e-25 104
Msa0313090 AT5G62940.1 82.000 50 9 0 46 95 74 123 1.42e-25 105
Msa0313090 AT1G64620.1 75.926 54 13 0 40 93 44 97 1.95e-25 104
Msa0313090 AT2G46590.1 78.431 51 11 0 42 92 65 115 2.32e-25 104
Msa0313090 AT5G39660.3 63.636 66 24 0 34 99 127 192 2.50e-25 105
Msa0313090 AT5G39660.1 63.636 66 24 0 34 99 127 192 2.50e-25 105
Msa0313090 AT5G39660.2 63.636 66 24 0 34 99 127 192 2.50e-25 105
Msa0313090 AT2G46590.2 78.431 51 11 0 42 92 77 127 2.75e-25 104
Msa0313090 AT3G47500.1 71.930 57 16 0 43 99 108 164 2.83e-25 105
Msa0313090 AT5G62430.1 49.425 87 36 1 22 100 23 109 8.89e-25 102
Msa0313090 AT1G26790.1 51.282 78 36 1 20 97 88 163 7.11e-24 100
Msa0313090 AT1G47655.1 72.549 51 14 0 43 93 27 77 8.34e-24 97.4
Msa0313090 AT4G21050.1 76.000 50 12 0 47 96 26 75 1.46e-23 96.7
Msa0313090 AT1G29160.1 56.923 65 28 0 38 102 55 119 2.85e-23 95.1
Msa0313090 AT2G34140.1 61.017 59 23 0 38 96 51 109 2.88e-23 94.7
Msa0313090 AT1G69570.1 61.017 59 23 0 39 97 126 184 1.22e-22 97.8
Msa0313090 AT4G21040.1 56.000 75 22 1 21 95 12 75 9.47e-22 92.4
Msa0313090 AT4G21080.1 53.333 75 24 1 21 95 12 75 1.24e-20 89.7
Msa0313090 AT4G21030.1 60.870 46 18 0 47 92 23 68 6.78e-13 67.0

Find 78 sgRNAs with CRISPR-Local

Find 204 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAATCCTTAGTTCAAGTTTA+AGG 0.109986 2_4:+8375733 Msa0313090:three_prime_UTR
GGGCTTGGAATTTAACAATT+TGG 0.140338 2_4:+8375522 Msa0313090:CDS
GCAAAGCTTGCAAACGTTAT+TGG 0.143992 2_4:+8374954 Msa0313090:CDS
AGGACTAATATTATGATTTA+AGG 0.162696 2_4:-8375155 None:intergenic
CACCAGCTTCTTCACTTTAC+TGG 0.188996 2_4:+8375614 Msa0313090:CDS
GGCTTGGAATTTAACAATTT+GGG 0.228469 2_4:+8375523 Msa0313090:CDS
GTTTAAGGTTATCTTTAATT+TGG 0.229380 2_4:+8375748 Msa0313090:three_prime_UTR
AATGATCAAGGTTCTAATAT+TGG 0.267697 2_4:+8375670 Msa0313090:CDS
ACTCTTCAAGGATTCTCTTT+TGG 0.307452 2_4:+8374481 Msa0313090:five_prime_UTR
TGTTGTCTGTATACTCTTCA+AGG 0.308452 2_4:+8374469 Msa0313090:five_prime_UTR
AATAGATGATGTTAAGATAA+TGG 0.329856 2_4:+8374805 Msa0313090:CDS
GCTTCAAGGGTTTCAAATAT+AGG 0.337429 2_4:-8374428 None:intergenic
TGTATAACCATTATGATCAT+TGG 0.338919 2_4:-8375338 None:intergenic
GGACAAGAGGTGGTACTCTT+AGG 0.348242 2_4:+8374975 Msa0313090:CDS
ACAATCTAACTGAGGTTTGC+TGG 0.378078 2_4:-8375996 None:intergenic
CATCATCAGCTATTGGTGTT+TGG 0.379698 2_4:+8375647 Msa0313090:CDS
TCTAGAATCTCAACAAGTTC+AGG 0.387293 2_4:+8375247 Msa0313090:CDS
ATAACCTTAAACTTGAACTA+AGG 0.401805 2_4:-8375737 None:intergenic
GTGGTGTTTGGTTTGGAAGT+AGG 0.405131 2_4:-8375118 None:intergenic
GATCATAATGGTTATACAAA+TGG 0.418978 2_4:+8375343 Msa0313090:CDS
ATTATGATCATTGGCTTCGC+TGG 0.424556 2_4:-8375329 None:intergenic
CTTAGGAATGTTCCTGTTGG+TGG 0.434008 2_4:+8374992 Msa0313090:CDS
GCAGTTGGTCACTAGCTTCA+AGG 0.440834 2_4:-8374442 None:intergenic
GAATTTGAAGAAGTGGTGTT+TGG 0.444730 2_4:-8375130 None:intergenic
ACAGCATCATCATCAGCTAT+TGG 0.454258 2_4:+8375640 Msa0313090:CDS
TGAAGAAGTGGTGTTTGGTT+TGG 0.459504 2_4:-8375125 None:intergenic
TTGGGGAATGGAAGTGAGAA+AGG 0.465690 2_4:+8375541 Msa0313090:CDS
AGAAGAATTAGTAGTATCAA+TGG 0.471958 2_4:-8375080 None:intergenic
AACCTGTGGCCATTGATTAG+TGG 0.473502 2_4:-8374553 None:intergenic
CTTCTGTAACCACCACCAAC+AGG 0.474357 2_4:-8375004 None:intergenic
ATTGGTGTTTGGAATGATCA+AGG 0.474683 2_4:+8375658 Msa0313090:CDS
GAATTTAACAATTTGGGGAA+TGG 0.481892 2_4:+8375529 Msa0313090:CDS
TAAGGTTGAATTTGAAGAAG+TGG 0.481982 2_4:-8375137 None:intergenic
CAACATAAATTCATGAATGA+TGG 0.486443 2_4:+8375484 Msa0313090:CDS
CAAGCTTTGCAGAAATGTCT+TGG 0.496012 2_4:-8374941 None:intergenic
CAGCCTCAGATGAATACCAT+TGG 0.500808 2_4:+8375280 Msa0313090:CDS
AAAGGAATGAAGAAAGATGA+AGG 0.500974 2_4:+8375559 Msa0313090:CDS
GAAGAAAGATGAAGGAGAGA+TGG 0.502257 2_4:+8375567 Msa0313090:CDS
TGAGCTAGAGGAGAGTACTT+TGG 0.503240 2_4:+8375946 Msa0313090:three_prime_UTR
GGCATAACTGATGCATTACT+TGG 0.503446 2_4:-8375436 None:intergenic
GAGATATTGAACCTTGAGAA+TGG 0.505952 2_4:-8375226 None:intergenic
CAGTTGGTCACTAGCTTCAA+GGG 0.509480 2_4:-8374441 None:intergenic
TTGGAAGTAGGAGTTGAAGA+AGG 0.509651 2_4:-8375106 None:intergenic
GGCCATTGATTAGTGGTTGG+AGG 0.513555 2_4:-8374546 None:intergenic
AGGAATGTTCCTGTTGGTGG+TGG 0.516563 2_4:+8374995 Msa0313090:CDS
TTGCTGGATGCAGCTTACAT+AGG 0.517627 2_4:-8375980 None:intergenic
AGCGAAGCCAATGATCATAA+TGG 0.521310 2_4:+8375331 Msa0313090:CDS
ATGTGAATCTTCCATTCTCA+AGG 0.533312 2_4:+8375215 Msa0313090:CDS
CTTGATAAACCATAAAACAT+AGG 0.540160 2_4:-8375175 None:intergenic
GGCTTCGCTGGACGTAAACC+CGG 0.542853 2_4:-8375317 None:intergenic
GCTTGGAATTTAACAATTTG+GGG 0.544684 2_4:+8375524 Msa0313090:CDS
ATTAGTAGTATCAATGGTGG+AGG 0.545112 2_4:-8375074 None:intergenic
GCCATTGATTAGTGGTTGGA+GGG 0.550319 2_4:-8374545 None:intergenic
AGAATTAGTAGTATCAATGG+TGG 0.550623 2_4:-8375077 None:intergenic
TCCCTCCAACCACTAATCAA+TGG 0.555533 2_4:+8374544 Msa0313090:CDS
GAAAGGTAAAGGTGAGCTAG+AGG 0.570168 2_4:+8375934 Msa0313090:three_prime_UTR
CAGATGAATACCATTGGAGT+AGG 0.575150 2_4:+8375286 Msa0313090:CDS
TACAGACAACAAAGAGCAGT+TGG 0.576785 2_4:-8374457 None:intergenic
TGCAAACGTTATTGGACAAG+AGG 0.578125 2_4:+8374962 Msa0313090:CDS
ACTCTTAGGAATGTTCCTGT+TGG 0.582456 2_4:+8374989 Msa0313090:CDS
TTCCAGTAAAGTGAAGAAGC+TGG 0.599918 2_4:-8375616 None:intergenic
TGAAGGAGAGATGGAACATG+TGG 0.611078 2_4:+8375576 Msa0313090:CDS
AACCACTAATCAATGGCCAC+AGG 0.613476 2_4:+8374551 Msa0313090:CDS
ACTCCAATGGTATTCATCTG+AGG 0.617945 2_4:-8375283 None:intergenic
TTTCAAATATAGGAGAGTGA+GGG 0.620308 2_4:-8374418 None:intergenic
GTTTCAAATATAGGAGAGTG+AGG 0.621310 2_4:-8374419 None:intergenic
AGAGCAGAACTCAACAGAGA+AGG 0.622062 2_4:-8375457 None:intergenic
TTCAAATATAGGAGAGTGAG+GGG 0.622411 2_4:-8374417 None:intergenic
GGATGAAAAGCCTACTCCAA+TGG 0.628438 2_4:-8375296 None:intergenic
AAACGTTATTGGACAAGAGG+TGG 0.639210 2_4:+8374965 Msa0313090:CDS
TGTGGCCATTGATTAGTGGT+TGG 0.640481 2_4:-8374549 None:intergenic
CAGTAAAGTGAAGAAGCTGG+TGG 0.648703 2_4:-8375613 None:intergenic
TAATAAGTAAAGCTAAACAA+AGG 0.672843 2_4:-8375773 None:intergenic
TAAAACTAACAATCTAACTG+AGG 0.677835 2_4:-8376004 None:intergenic
GAAGGAGAGATGGAACATGT+GGG 0.678274 2_4:+8375577 Msa0313090:CDS
TTAGTAGTATCAATGGTGGA+GGG 0.679047 2_4:-8375073 None:intergenic
GGAGAGATGGAACATGTGGG+TGG 0.710445 2_4:+8375580 Msa0313090:CDS
GTGTTTGATGAGTCACAACG+TGG 0.749573 2_4:-8374881 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATAATTGATATAAGAAAA+AGG - chr2_4:8374751-8374770 None:intergenic 10.0%
!! ATATAATTGATATAAGAAAA+AGG - chr2_4:8374751-8374770 None:intergenic 10.0%
!!! TTTGTTTTGTTTTGTATTTT+TGG + chr2_4:8375832-8375851 Msa0313090:three_prime_UTR 15.0%
!!! TTGTTTTGTTTTGTATTTTT+GGG + chr2_4:8375833-8375852 Msa0313090:three_prime_UTR 15.0%
!! TTATTGAAAAAAGAAAAAGA+AGG + chr2_4:8375878-8375897 Msa0313090:three_prime_UTR 15.0%
!!! TTTGTTTTGTTTTGTATTTT+TGG + chr2_4:8375832-8375851 Msa0313090:three_prime_UTR 15.0%
!!! TTGTTTTGTTTTGTATTTTT+GGG + chr2_4:8375833-8375852 Msa0313090:three_prime_UTR 15.0%
!! TTATTGAAAAAAGAAAAAGA+AGG + chr2_4:8375878-8375897 Msa0313090:three_prime_UTR 15.0%
!! AATAGATGATGTTAAGATAA+TGG + chr2_4:8374805-8374824 Msa0313090:CDS 20.0%
!! AGGACTAATATTATGATTTA+AGG - chr2_4:8375158-8375177 None:intergenic 20.0%
!!! AATATTAGTCCTATGTTTTA+TGG + chr2_4:8375166-8375185 Msa0313090:CDS 20.0%
!!! GTTTAAGGTTATCTTTAATT+TGG + chr2_4:8375748-8375767 Msa0313090:three_prime_UTR 20.0%
!! TAATAAGTAAAGCTAAACAA+AGG - chr2_4:8375776-8375795 None:intergenic 20.0%
!! AAAAGAAGGAAGAAAAAAAA+AGG + chr2_4:8375892-8375911 Msa0313090:three_prime_UTR 20.0%
!! AATAGATGATGTTAAGATAA+TGG + chr2_4:8374805-8374824 Msa0313090:CDS 20.0%
!! AGGACTAATATTATGATTTA+AGG - chr2_4:8375158-8375177 None:intergenic 20.0%
!!! AATATTAGTCCTATGTTTTA+TGG + chr2_4:8375166-8375185 Msa0313090:CDS 20.0%
!!! GTTTAAGGTTATCTTTAATT+TGG + chr2_4:8375748-8375767 Msa0313090:three_prime_UTR 20.0%
!! TAATAAGTAAAGCTAAACAA+AGG - chr2_4:8375776-8375795 None:intergenic 20.0%
!! AAAAGAAGGAAGAAAAAAAA+AGG + chr2_4:8375892-8375911 Msa0313090:three_prime_UTR 20.0%
! TTTGTGTAAATAATTGTGCA+TGG - chr2_4:8374610-8374629 None:intergenic 25.0%
! AGCTAAGCTACAAAATTAAT+TGG - chr2_4:8374646-8374665 None:intergenic 25.0%
!!! TAATTTTGTAGCTTAGCTTT+TGG + chr2_4:8374648-8374667 Msa0313090:intron 25.0%
!! AGTTTAATAAGTTCTAGCTA+GGG - chr2_4:8374717-8374736 None:intergenic 25.0%
!! TAGTTTAATAAGTTCTAGCT+AGG - chr2_4:8374718-8374737 None:intergenic 25.0%
!!! GTTAAGATAATGGAAAAACA+AGG + chr2_4:8374815-8374834 Msa0313090:CDS 25.0%
! AGAAGAATTAGTAGTATCAA+TGG - chr2_4:8375083-8375102 None:intergenic 25.0%
! CTTGATAAACCATAAAACAT+AGG - chr2_4:8375178-8375197 None:intergenic 25.0%
! TGTATAACCATTATGATCAT+TGG - chr2_4:8375341-8375360 None:intergenic 25.0%
! GATCATAATGGTTATACAAA+TGG + chr2_4:8375343-8375362 Msa0313090:CDS 25.0%
! CAACATAAATTCATGAATGA+TGG + chr2_4:8375484-8375503 Msa0313090:CDS 25.0%
!! AATGATCAAGGTTCTAATAT+TGG + chr2_4:8375670-8375689 Msa0313090:CDS 25.0%
! ATAACCTTAAACTTGAACTA+AGG - chr2_4:8375740-8375759 None:intergenic 25.0%
!!! TTTGTTTTGTATTTTTGGGT+TGG + chr2_4:8375837-8375856 Msa0313090:three_prime_UTR 25.0%
!!! TTGTTTTGTATTTTTGGGTT+GGG + chr2_4:8375838-8375857 Msa0313090:three_prime_UTR 25.0%
!! AAGGATACTTGTTAGAAAAA+TGG + chr2_4:8375911-8375930 Msa0313090:three_prime_UTR 25.0%
!! AGGATACTTGTTAGAAAAAT+GGG + chr2_4:8375912-8375931 Msa0313090:three_prime_UTR 25.0%
! TAAAACTAACAATCTAACTG+AGG - chr2_4:8376007-8376026 None:intergenic 25.0%
! TTTGTGTAAATAATTGTGCA+TGG - chr2_4:8374610-8374629 None:intergenic 25.0%
! AGCTAAGCTACAAAATTAAT+TGG - chr2_4:8374646-8374665 None:intergenic 25.0%
!!! TAATTTTGTAGCTTAGCTTT+TGG + chr2_4:8374648-8374667 Msa0313090:intron 25.0%
!! AGTTTAATAAGTTCTAGCTA+GGG - chr2_4:8374717-8374736 None:intergenic 25.0%
!! TAGTTTAATAAGTTCTAGCT+AGG - chr2_4:8374718-8374737 None:intergenic 25.0%
!!! GTTAAGATAATGGAAAAACA+AGG + chr2_4:8374815-8374834 Msa0313090:CDS 25.0%
! AGAAGAATTAGTAGTATCAA+TGG - chr2_4:8375083-8375102 None:intergenic 25.0%
! CTTGATAAACCATAAAACAT+AGG - chr2_4:8375178-8375197 None:intergenic 25.0%
! TGTATAACCATTATGATCAT+TGG - chr2_4:8375341-8375360 None:intergenic 25.0%
! GATCATAATGGTTATACAAA+TGG + chr2_4:8375343-8375362 Msa0313090:CDS 25.0%
! CAACATAAATTCATGAATGA+TGG + chr2_4:8375484-8375503 Msa0313090:CDS 25.0%
!! AATGATCAAGGTTCTAATAT+TGG + chr2_4:8375670-8375689 Msa0313090:CDS 25.0%
! ATAACCTTAAACTTGAACTA+AGG - chr2_4:8375740-8375759 None:intergenic 25.0%
!!! TTTGTTTTGTATTTTTGGGT+TGG + chr2_4:8375837-8375856 Msa0313090:three_prime_UTR 25.0%
!!! TTGTTTTGTATTTTTGGGTT+GGG + chr2_4:8375838-8375857 Msa0313090:three_prime_UTR 25.0%
!! AAGGATACTTGTTAGAAAAA+TGG + chr2_4:8375911-8375930 Msa0313090:three_prime_UTR 25.0%
!! AGGATACTTGTTAGAAAAAT+GGG + chr2_4:8375912-8375931 Msa0313090:three_prime_UTR 25.0%
! TAAAACTAACAATCTAACTG+AGG - chr2_4:8376007-8376026 None:intergenic 25.0%
AGAATTAGTAGTATCAATGG+TGG - chr2_4:8375080-8375099 None:intergenic 30.0%
TAAGGTTGAATTTGAAGAAG+TGG - chr2_4:8375140-8375159 None:intergenic 30.0%
GGCTTGGAATTTAACAATTT+GGG + chr2_4:8375523-8375542 Msa0313090:CDS 30.0%
GCTTGGAATTTAACAATTTG+GGG + chr2_4:8375524-8375543 Msa0313090:CDS 30.0%
GAATTTAACAATTTGGGGAA+TGG + chr2_4:8375529-8375548 Msa0313090:CDS 30.0%
AAAGGAATGAAGAAAGATGA+AGG + chr2_4:8375559-8375578 Msa0313090:CDS 30.0%
! CAATCCTTAGTTCAAGTTTA+AGG + chr2_4:8375733-8375752 Msa0313090:three_prime_UTR 30.0%
! ACTTGTTAGAAAAATGGGAA+AGG + chr2_4:8375917-8375936 Msa0313090:three_prime_UTR 30.0%
TAGAAAAATGGGAAAGGTAA+AGG + chr2_4:8375923-8375942 Msa0313090:three_prime_UTR 30.0%
AGAATTAGTAGTATCAATGG+TGG - chr2_4:8375080-8375099 None:intergenic 30.0%
TAAGGTTGAATTTGAAGAAG+TGG - chr2_4:8375140-8375159 None:intergenic 30.0%
GGCTTGGAATTTAACAATTT+GGG + chr2_4:8375523-8375542 Msa0313090:CDS 30.0%
GCTTGGAATTTAACAATTTG+GGG + chr2_4:8375524-8375543 Msa0313090:CDS 30.0%
GAATTTAACAATTTGGGGAA+TGG + chr2_4:8375529-8375548 Msa0313090:CDS 30.0%
AAAGGAATGAAGAAAGATGA+AGG + chr2_4:8375559-8375578 Msa0313090:CDS 30.0%
! CAATCCTTAGTTCAAGTTTA+AGG + chr2_4:8375733-8375752 Msa0313090:three_prime_UTR 30.0%
! ACTTGTTAGAAAAATGGGAA+AGG + chr2_4:8375917-8375936 Msa0313090:three_prime_UTR 30.0%
TAGAAAAATGGGAAAGGTAA+AGG + chr2_4:8375923-8375942 Msa0313090:three_prime_UTR 30.0%
GTTTCAAATATAGGAGAGTG+AGG - chr2_4:8374422-8374441 None:intergenic 35.0%
GCTTCAAGGGTTTCAAATAT+AGG - chr2_4:8374431-8374450 None:intergenic 35.0%
TGTTGTCTGTATACTCTTCA+AGG + chr2_4:8374469-8374488 Msa0313090:five_prime_UTR 35.0%
! ACTCTTCAAGGATTCTCTTT+TGG + chr2_4:8374481-8374500 Msa0313090:five_prime_UTR 35.0%
! TTAGTAGTATCAATGGTGGA+GGG - chr2_4:8375076-8375095 None:intergenic 35.0%
ATTAGTAGTATCAATGGTGG+AGG - chr2_4:8375077-8375096 None:intergenic 35.0%
GAATTTGAAGAAGTGGTGTT+TGG - chr2_4:8375133-8375152 None:intergenic 35.0%
ATGTGAATCTTCCATTCTCA+AGG + chr2_4:8375215-8375234 Msa0313090:CDS 35.0%
GAGATATTGAACCTTGAGAA+TGG - chr2_4:8375229-8375248 None:intergenic 35.0%
! TCTAGAATCTCAACAAGTTC+AGG + chr2_4:8375247-8375266 Msa0313090:CDS 35.0%
GGGCTTGGAATTTAACAATT+TGG + chr2_4:8375522-8375541 Msa0313090:CDS 35.0%
! ATTGGTGTTTGGAATGATCA+AGG + chr2_4:8375658-8375677 Msa0313090:CDS 35.0%
GTTTCAAATATAGGAGAGTG+AGG - chr2_4:8374422-8374441 None:intergenic 35.0%
GCTTCAAGGGTTTCAAATAT+AGG - chr2_4:8374431-8374450 None:intergenic 35.0%
TGTTGTCTGTATACTCTTCA+AGG + chr2_4:8374469-8374488 Msa0313090:five_prime_UTR 35.0%
! ACTCTTCAAGGATTCTCTTT+TGG + chr2_4:8374481-8374500 Msa0313090:five_prime_UTR 35.0%
! TTAGTAGTATCAATGGTGGA+GGG - chr2_4:8375076-8375095 None:intergenic 35.0%
ATTAGTAGTATCAATGGTGG+AGG - chr2_4:8375077-8375096 None:intergenic 35.0%
GAATTTGAAGAAGTGGTGTT+TGG - chr2_4:8375133-8375152 None:intergenic 35.0%
ATGTGAATCTTCCATTCTCA+AGG + chr2_4:8375215-8375234 Msa0313090:CDS 35.0%
GAGATATTGAACCTTGAGAA+TGG - chr2_4:8375229-8375248 None:intergenic 35.0%
! TCTAGAATCTCAACAAGTTC+AGG + chr2_4:8375247-8375266 Msa0313090:CDS 35.0%
GGGCTTGGAATTTAACAATT+TGG + chr2_4:8375522-8375541 Msa0313090:CDS 35.0%
! ATTGGTGTTTGGAATGATCA+AGG + chr2_4:8375658-8375677 Msa0313090:CDS 35.0%
TACAGACAACAAAGAGCAGT+TGG - chr2_4:8374460-8374479 None:intergenic 40.0%
CAAGCTTTGCAGAAATGTCT+TGG - chr2_4:8374944-8374963 None:intergenic 40.0%
GCAAAGCTTGCAAACGTTAT+TGG + chr2_4:8374954-8374973 Msa0313090:CDS 40.0%
TGCAAACGTTATTGGACAAG+AGG + chr2_4:8374962-8374981 Msa0313090:CDS 40.0%
AAACGTTATTGGACAAGAGG+TGG + chr2_4:8374965-8374984 Msa0313090:CDS 40.0%
ACTCTTAGGAATGTTCCTGT+TGG + chr2_4:8374989-8375008 Msa0313090:CDS 40.0%
TTGGAAGTAGGAGTTGAAGA+AGG - chr2_4:8375109-8375128 None:intergenic 40.0%
TGAAGAAGTGGTGTTTGGTT+TGG - chr2_4:8375128-8375147 None:intergenic 40.0%
ACTCCAATGGTATTCATCTG+AGG - chr2_4:8375286-8375305 None:intergenic 40.0%
CAGATGAATACCATTGGAGT+AGG + chr2_4:8375286-8375305 Msa0313090:CDS 40.0%
! ATTGGAGTAGGCTTTTCATC+CGG + chr2_4:8375298-8375317 Msa0313090:CDS 40.0%
!! ATTATGATCATTGGCTTCGC+TGG - chr2_4:8375332-8375351 None:intergenic 40.0%
AGCGAAGCCAATGATCATAA+TGG + chr2_4:8375331-8375350 Msa0313090:CDS 40.0%
GGCATAACTGATGCATTACT+TGG - chr2_4:8375439-8375458 None:intergenic 40.0%
! TGATGGTAGCAACACTTTTC+AGG + chr2_4:8375501-8375520 Msa0313090:CDS 40.0%
! GATGGTAGCAACACTTTTCA+GGG + chr2_4:8375502-8375521 Msa0313090:CDS 40.0%
GAAGAAAGATGAAGGAGAGA+TGG + chr2_4:8375567-8375586 Msa0313090:CDS 40.0%
TTCCAGTAAAGTGAAGAAGC+TGG - chr2_4:8375619-8375638 None:intergenic 40.0%
ACAGCATCATCATCAGCTAT+TGG + chr2_4:8375640-8375659 Msa0313090:CDS 40.0%
! CATCATCAGCTATTGGTGTT+TGG + chr2_4:8375647-8375666 Msa0313090:CDS 40.0%
ACAATCTAACTGAGGTTTGC+TGG - chr2_4:8375999-8376018 None:intergenic 40.0%
TACAGACAACAAAGAGCAGT+TGG - chr2_4:8374460-8374479 None:intergenic 40.0%
CAAGCTTTGCAGAAATGTCT+TGG - chr2_4:8374944-8374963 None:intergenic 40.0%
GCAAAGCTTGCAAACGTTAT+TGG + chr2_4:8374954-8374973 Msa0313090:CDS 40.0%
TGCAAACGTTATTGGACAAG+AGG + chr2_4:8374962-8374981 Msa0313090:CDS 40.0%
AAACGTTATTGGACAAGAGG+TGG + chr2_4:8374965-8374984 Msa0313090:CDS 40.0%
ACTCTTAGGAATGTTCCTGT+TGG + chr2_4:8374989-8375008 Msa0313090:CDS 40.0%
TTGGAAGTAGGAGTTGAAGA+AGG - chr2_4:8375109-8375128 None:intergenic 40.0%
TGAAGAAGTGGTGTTTGGTT+TGG - chr2_4:8375128-8375147 None:intergenic 40.0%
ACTCCAATGGTATTCATCTG+AGG - chr2_4:8375286-8375305 None:intergenic 40.0%
CAGATGAATACCATTGGAGT+AGG + chr2_4:8375286-8375305 Msa0313090:CDS 40.0%
! ATTGGAGTAGGCTTTTCATC+CGG + chr2_4:8375298-8375317 Msa0313090:CDS 40.0%
!! ATTATGATCATTGGCTTCGC+TGG - chr2_4:8375332-8375351 None:intergenic 40.0%
AGCGAAGCCAATGATCATAA+TGG + chr2_4:8375331-8375350 Msa0313090:CDS 40.0%
GGCATAACTGATGCATTACT+TGG - chr2_4:8375439-8375458 None:intergenic 40.0%
! TGATGGTAGCAACACTTTTC+AGG + chr2_4:8375501-8375520 Msa0313090:CDS 40.0%
! GATGGTAGCAACACTTTTCA+GGG + chr2_4:8375502-8375521 Msa0313090:CDS 40.0%
GAAGAAAGATGAAGGAGAGA+TGG + chr2_4:8375567-8375586 Msa0313090:CDS 40.0%
TTCCAGTAAAGTGAAGAAGC+TGG - chr2_4:8375619-8375638 None:intergenic 40.0%
ACAGCATCATCATCAGCTAT+TGG + chr2_4:8375640-8375659 Msa0313090:CDS 40.0%
! CATCATCAGCTATTGGTGTT+TGG + chr2_4:8375647-8375666 Msa0313090:CDS 40.0%
ACAATCTAACTGAGGTTTGC+TGG - chr2_4:8375999-8376018 None:intergenic 40.0%
! CAGTTGGTCACTAGCTTCAA+GGG - chr2_4:8374444-8374463 None:intergenic 45.0%
TCCCTCCAACCACTAATCAA+TGG + chr2_4:8374544-8374563 Msa0313090:CDS 45.0%
! GCCATTGATTAGTGGTTGGA+GGG - chr2_4:8374548-8374567 None:intergenic 45.0%
! TGTGGCCATTGATTAGTGGT+TGG - chr2_4:8374552-8374571 None:intergenic 45.0%
AACCACTAATCAATGGCCAC+AGG + chr2_4:8374551-8374570 Msa0313090:CDS 45.0%
! AACCTGTGGCCATTGATTAG+TGG - chr2_4:8374556-8374575 None:intergenic 45.0%
GATAGAGAGAGAGAGAGAGA+GGG - chr2_4:8374586-8374605 None:intergenic 45.0%
AGATAGAGAGAGAGAGAGAG+AGG - chr2_4:8374587-8374606 None:intergenic 45.0%
GTGTTTGATGAGTCACAACG+TGG - chr2_4:8374884-8374903 None:intergenic 45.0%
CTTAGGAATGTTCCTGTTGG+TGG + chr2_4:8374992-8375011 Msa0313090:CDS 45.0%
!! GTGGTGTTTGGTTTGGAAGT+AGG - chr2_4:8375121-8375140 None:intergenic 45.0%
CAGCCTCAGATGAATACCAT+TGG + chr2_4:8375280-8375299 Msa0313090:CDS 45.0%
GGATGAAAAGCCTACTCCAA+TGG - chr2_4:8375299-8375318 None:intergenic 45.0%
! TTGGAGTAGGCTTTTCATCC+GGG + chr2_4:8375299-8375318 Msa0313090:CDS 45.0%
AGAGCAGAACTCAACAGAGA+AGG - chr2_4:8375460-8375479 None:intergenic 45.0%
!! TAGCAACACTTTTCAGGGCT+TGG + chr2_4:8375507-8375526 Msa0313090:CDS 45.0%
TTGGGGAATGGAAGTGAGAA+AGG + chr2_4:8375541-8375560 Msa0313090:CDS 45.0%
TGAAGGAGAGATGGAACATG+TGG + chr2_4:8375576-8375595 Msa0313090:CDS 45.0%
GAAGGAGAGATGGAACATGT+GGG + chr2_4:8375577-8375596 Msa0313090:CDS 45.0%
CAGTAAAGTGAAGAAGCTGG+TGG - chr2_4:8375616-8375635 None:intergenic 45.0%
CACCAGCTTCTTCACTTTAC+TGG + chr2_4:8375614-8375633 Msa0313090:CDS 45.0%
GAAAGGTAAAGGTGAGCTAG+AGG + chr2_4:8375934-8375953 Msa0313090:three_prime_UTR 45.0%
!! TGAGCTAGAGGAGAGTACTT+TGG + chr2_4:8375946-8375965 Msa0313090:three_prime_UTR 45.0%
TTGCTGGATGCAGCTTACAT+AGG - chr2_4:8375983-8376002 None:intergenic 45.0%
! CAGTTGGTCACTAGCTTCAA+GGG - chr2_4:8374444-8374463 None:intergenic 45.0%
TCCCTCCAACCACTAATCAA+TGG + chr2_4:8374544-8374563 Msa0313090:CDS 45.0%
! GCCATTGATTAGTGGTTGGA+GGG - chr2_4:8374548-8374567 None:intergenic 45.0%
! TGTGGCCATTGATTAGTGGT+TGG - chr2_4:8374552-8374571 None:intergenic 45.0%
AACCACTAATCAATGGCCAC+AGG + chr2_4:8374551-8374570 Msa0313090:CDS 45.0%
! AACCTGTGGCCATTGATTAG+TGG - chr2_4:8374556-8374575 None:intergenic 45.0%
GATAGAGAGAGAGAGAGAGA+GGG - chr2_4:8374586-8374605 None:intergenic 45.0%
AGATAGAGAGAGAGAGAGAG+AGG - chr2_4:8374587-8374606 None:intergenic 45.0%
GTGTTTGATGAGTCACAACG+TGG - chr2_4:8374884-8374903 None:intergenic 45.0%
CTTAGGAATGTTCCTGTTGG+TGG + chr2_4:8374992-8375011 Msa0313090:CDS 45.0%
!! GTGGTGTTTGGTTTGGAAGT+AGG - chr2_4:8375121-8375140 None:intergenic 45.0%
CAGCCTCAGATGAATACCAT+TGG + chr2_4:8375280-8375299 Msa0313090:CDS 45.0%
GGATGAAAAGCCTACTCCAA+TGG - chr2_4:8375299-8375318 None:intergenic 45.0%
! TTGGAGTAGGCTTTTCATCC+GGG + chr2_4:8375299-8375318 Msa0313090:CDS 45.0%
AGAGCAGAACTCAACAGAGA+AGG - chr2_4:8375460-8375479 None:intergenic 45.0%
!! TAGCAACACTTTTCAGGGCT+TGG + chr2_4:8375507-8375526 Msa0313090:CDS 45.0%
TTGGGGAATGGAAGTGAGAA+AGG + chr2_4:8375541-8375560 Msa0313090:CDS 45.0%
TGAAGGAGAGATGGAACATG+TGG + chr2_4:8375576-8375595 Msa0313090:CDS 45.0%
GAAGGAGAGATGGAACATGT+GGG + chr2_4:8375577-8375596 Msa0313090:CDS 45.0%
CAGTAAAGTGAAGAAGCTGG+TGG - chr2_4:8375616-8375635 None:intergenic 45.0%
CACCAGCTTCTTCACTTTAC+TGG + chr2_4:8375614-8375633 Msa0313090:CDS 45.0%
GAAAGGTAAAGGTGAGCTAG+AGG + chr2_4:8375934-8375953 Msa0313090:three_prime_UTR 45.0%
!! TGAGCTAGAGGAGAGTACTT+TGG + chr2_4:8375946-8375965 Msa0313090:three_prime_UTR 45.0%
TTGCTGGATGCAGCTTACAT+AGG - chr2_4:8375983-8376002 None:intergenic 45.0%
! GCAGTTGGTCACTAGCTTCA+AGG - chr2_4:8374445-8374464 None:intergenic 50.0%
! GGCCATTGATTAGTGGTTGG+AGG - chr2_4:8374549-8374568 None:intergenic 50.0%
GGACAAGAGGTGGTACTCTT+AGG + chr2_4:8374975-8374994 Msa0313090:CDS 50.0%
! AGGAATGTTCCTGTTGGTGG+TGG + chr2_4:8374995-8375014 Msa0313090:CDS 50.0%
CTTCTGTAACCACCACCAAC+AGG - chr2_4:8375007-8375026 None:intergenic 50.0%
! GCAGTTGGTCACTAGCTTCA+AGG - chr2_4:8374445-8374464 None:intergenic 50.0%
! GGCCATTGATTAGTGGTTGG+AGG - chr2_4:8374549-8374568 None:intergenic 50.0%
GGACAAGAGGTGGTACTCTT+AGG + chr2_4:8374975-8374994 Msa0313090:CDS 50.0%
! AGGAATGTTCCTGTTGGTGG+TGG + chr2_4:8374995-8375014 Msa0313090:CDS 50.0%
CTTCTGTAACCACCACCAAC+AGG - chr2_4:8375007-8375026 None:intergenic 50.0%
GAGAGGGAGAGATGAACCTG+TGG - chr2_4:8374570-8374589 None:intergenic 55.0%
GGAGAGATGGAACATGTGGG+TGG + chr2_4:8375580-8375599 Msa0313090:CDS 55.0%
GAGAGGGAGAGATGAACCTG+TGG - chr2_4:8374570-8374589 None:intergenic 55.0%
GGAGAGATGGAACATGTGGG+TGG + chr2_4:8375580-8375599 Msa0313090:CDS 55.0%
GGCTTCGCTGGACGTAAACC+CGG - chr2_4:8375320-8375339 None:intergenic 60.0%
GGCTTCGCTGGACGTAAACC+CGG - chr2_4:8375320-8375339 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 8374419 8376032 8374419 ID=Msa0313090;Name=Msa0313090
chr2_4 mRNA 8374419 8376032 8374419 ID=Msa0313090-mRNA-1;Parent=Msa0313090;Name=Msa0313090-mRNA-1;_AED=0.00;_eAED=0.00;_QI=94|1|1|1|1|1|2|315|325
chr2_4 exon 8374419 8374572 8374419 ID=Msa0313090-mRNA-1:exon:3187;Parent=Msa0313090-mRNA-1
chr2_4 exon 8374800 8376032 8374800 ID=Msa0313090-mRNA-1:exon:3188;Parent=Msa0313090-mRNA-1
chr2_4 five_prime_UTR 8374419 8374512 8374419 ID=Msa0313090-mRNA-1:five_prime_utr;Parent=Msa0313090-mRNA-1
chr2_4 CDS 8374513 8374572 8374513 ID=Msa0313090-mRNA-1:cds;Parent=Msa0313090-mRNA-1
chr2_4 CDS 8374800 8375717 8374800 ID=Msa0313090-mRNA-1:cds;Parent=Msa0313090-mRNA-1
chr2_4 three_prime_UTR 8375718 8376032 8375718 ID=Msa0313090-mRNA-1:three_prime_utr;Parent=Msa0313090-mRNA-1
Gene Sequence

>Msa0313090

ATGTTATCTAACTATGAGAAAATGCTTGTCATCCCTCCAACCACTAATCAATGGCCACAGAATCAAATAGATGATGTTAAGATAATGGAAAAACAAGGTCAAGAACTTCAGCAGCAGCAGCAACAACAAGCTCTCAAGTGTCCACGTTGTGACTCATCAAACACAAAGTTTTGTTACTACAACAACTACAGTTTGTCACAACCAAGACATTTCTGCAAAGCTTGCAAACGTTATTGGACAAGAGGTGGTACTCTTAGGAATGTTCCTGTTGGTGGTGGTTACAGAAGAAACAACTCAAACAAACGTAGTAACAATACTACTCTCATCAAAAGACCCTCCACCATTGATACTACTAATTCTTCTTCTCCTTCTTCAACTCCTACTTCCAAACCAAACACCACTTCTTCAAATTCAACCTTAAATCATAATATTAGTCCTATGTTTTATGGTTTATCAAGTAGTAATAACTCTTGTGATGTGAATCTTCCATTCTCAAGGTTCAATATCTCTAGAATCTCAACAAGTTCAGGTTATGATTTTCAGCCTCAGATGAATACCATTGGAGTAGGCTTTTCATCCGGGTTTACGTCCAGCGAAGCCAATGATCATAATGGTTATACAAATGGATTTACTTCAAATAATAATAATAACACTCTTGTCTCAAGATATGATTCAATCTTTAGCTCTTCTTCTGCACCAAGTAATGCATCAGTTATGCCTTCTCTGTTGAGTTCTGCTCTGCTGCAACATAAATTCATGAATGATGGTAGCAACACTTTTCAGGGCTTGGAATTTAACAATTTGGGGAATGGAAGTGAGAAAGGAATGAAGAAAGATGAAGGAGAGATGGAACATGTGGGTGGTTTGTATGATCCACCAGCTTCTTCACTTTACTGGAATACAGCATCATCATCAGCTATTGGTGTTTGGAATGATCAAGGTTCTAATATTGGTTCATCAGTTACTTCTTTGATCTAG
Protein sequence

>Msa0313090

MLSNYEKMLVIPPTTNQWPQNQIDDVKIMEKQGQELQQQQQQQALKCPRCDSSNTKFCYYNNYSLSQPRHFCKACKRYWTRGGTLRNVPVGGGYRRNNSNKRSNNTTLIKRPSTIDTTNSSSPSSTPTSKPNTTSSNSTLNHNISPMFYGLSSSNNSCDVNLPFSRFNISRISTSSGYDFQPQMNTIGVGFSSGFTSSEANDHNGYTNGFTSNNNNNTLVSRYDSIFSSSSAPSNASVMPSLLSSALLQHKFMNDGSNTFQGLEFNNLGNGSEKGMKKDEGEMEHVGGLYDPPASSLYWNTASSSAIGVWNDQGSNIGSSVTSLI*