AlfalfaGEDB | Msa0316060

M. sativa cultivar zhongmu-4 / Msa0316060

KEGG: K04730
GO: GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
PFAM: PF08263.15

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0316060	XP_013462818.2	95.462	617	28	0	1	617	1	617	0.0	1224

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0316060	sp\|O48788\|Y2267_ARATH	36.760	642	325	20	16	609	15	623	2.41e-102	327

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0316060	MtrunA17_Chr2g0288391	95.462	617	28	0	1	617	1	617	0.0	1224
Msa0316060	MtrunA17_Chr8g0376301	84.186	430	19	3	190	617	9	391	0.0	717
Msa0316060	MtrunA17_Chr3g0117921	46.785	622	295	10	25	615	25	641	0.0	540
Msa0316060	MtrunA17_Chr3g0128551	34.161	644	360	18	4	611	5	620	3.40e-97	310
Msa0316060	MtrunA17_Chr3g0108301	32.915	638	368	19	7	612	24	633	2.95e-94	303
Msa0316060	MtrunA17_Chr1g0209521	35.008	637	322	22	36	609	34	641	1.92e-93	301
Msa0316060	MtrunA17_Chr5g0423121	33.807	633	351	21	10	607	10	609	1.54e-92	298
Msa0316060	MtrunA17_Chr7g0245171	34.961	635	339	23	7	606	12	607	7.74e-92	295
Msa0316060	MtrunA17_Chr5g0432851	33.863	629	361	19	7	609	32	631	9.32e-89	288
Msa0316060	MtrunA17_Chr8g0387791	33.010	618	342	19	23	606	17	596	3.85e-88	286

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0316060	AT2G26730.1	36.760	642	325	20	16	609	15	623	2.45e-103	327
Msa0316060	AT1G68400.1	34.154	609	325	18	52	606	51	637	4.23e-89	290
Msa0316060	AT3G08680.1	33.178	645	361	26	1	606	2	615	5.21e-87	284
Msa0316060	AT3G08680.2	33.178	645	361	26	1	606	2	615	5.21e-87	284
Msa0316060	AT5G58300.3	33.646	639	357	21	4	606	23	630	3.30e-86	282
Msa0316060	AT5G58300.1	33.646	639	357	21	4	606	23	630	3.30e-86	282
Msa0316060	AT5G58300.2	33.646	639	357	21	4	606	23	630	3.30e-86	282
Msa0316060	AT5G53320.1	31.553	618	359	16	7	609	5	573	1.57e-83	274
Msa0316060	AT1G64210.1	32.432	629	347	18	7	606	3	582	7.31e-82	269
Msa0316060	AT5G05160.1	32.343	606	344	19	50	616	49	627	2.64e-81	269

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Find 113 sgRNAs with CRISPR-Local

Find 310 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAATGGAAGTCTTTATA+AGG	0.363606	2_4:-12854033	Msa0316060:CDS
AAATTAGAGAAATAAAATGT+TGG	0.548949	2_4:+12854551	None:intergenic
AAGAAATAACCTTGCAAATC+TGG	0.240365	2_4:+12854485	None:intergenic
AAGAGTATGCAAGTGAAGAG+AGG	0.544291	2_4:-12852827	Msa0316060:CDS
AAGGAAGATGTTTATGGATT+TGG	0.251168	2_4:-12852975	Msa0316060:CDS
AAGGTGAGAGATTCAGCTAG+AGG	0.622866	2_4:+12854824	None:intergenic
AAGTCTGCTGCTCCAATCAA+AGG	0.506331	2_4:+12853199	None:intergenic
AAGTTGAATTATCATTCTGC+TGG	0.497084	2_4:+12854990	None:intergenic
AATATCAGAACAAGAATCAA+AGG	0.504947	2_4:-12854375	Msa0316060:CDS
AATCAGTTGTAAGGGAAGAA+TGG	0.497333	2_4:-12852881	Msa0316060:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TAAGAAGACCAACAAGATAT+TGG	+	chr2_4:12852708-12852727	None:intergenic	30.0%
ATCATGTTTAGGAGGAAAAA+GGG	+	chr2_4:12852737-12852756	None:intergenic	30.0%
AATCATGTTTAGGAGGAAAA+AGG	+	chr2_4:12852738-12852757	None:intergenic	30.0%
TCTCACAACTAATCATGTTT+AGG	+	chr2_4:12852748-12852767	None:intergenic	30.0%
AAGTTGAATTATCATTCTGC+TGG	+	chr2_4:12852820-12852839	None:intergenic	30.0%
GCAGAATGATAATTCAACTT+TGG	-	chr2_4:12852820-12852839	Msa0316060:CDS	30.0%
ATGATAATTCAACTTTGGTC+TGG	-	chr2_4:12852825-12852844	Msa0316060:CDS	30.0%
TAAAGAAGTTGGATCTTTAC+AGG	-	chr2_4:12852912-12852931	Msa0316060:CDS	30.0%
TGGACCACTAAAATTGTTAA+AGG	+	chr2_4:12853299-12853318	None:intergenic	30.0%
AAGAAATAACCTTGCAAATC+TGG	+	chr2_4:12853325-12853344	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_4	gene	12852692	12855137	12852692	ID=Msa0316060;Name=Msa0316060
chr2_4	mRNA	12852692	12855137	12852692	ID=Msa0316060-mRNA-1;Parent=Msa0316060;Name=Msa0316060-mRNA-1;_AED=0.32;_eAED=0.32;_QI=0\|0\|0\|1\|1\|1\|2\|0\|617
chr2_4	exon	12853826	12855137	12853826	ID=Msa0316060-mRNA-1:exon:4893;Parent=Msa0316060-mRNA-1
chr2_4	exon	12852692	12853233	12852692	ID=Msa0316060-mRNA-1:exon:4892;Parent=Msa0316060-mRNA-1
chr2_4	CDS	12853826	12855137	12853826	ID=Msa0316060-mRNA-1:cds;Parent=Msa0316060-mRNA-1
chr2_4	CDS	12852692	12853233	12852692	ID=Msa0316060-mRNA-1:cds;Parent=Msa0316060-mRNA-1

Gene Sequence

>Msa0316060

ATGGAAATCATTTCCAATATCTTGTTGGTCTTCTTAGTAACACCCTTTTTCCTCCTAAACATGATTAGTTGTGAGAATGATGAGATGAAGAGAACACTAATACAGTTCTTGACACAACTCAAAGGCCAGCAGAATGATAATTCAACTTTGGTCTGGAAACCAGACACTGATCCCTGCAAGGATCATTGGAATGGTGTATACTGTGATGCACAAATGTCAATAAAGAAGTTGGATCTTTACAGGTTCAATTTGTCTGGAACTCTCGACGTTGCCTTGCTTTGCAACCTGCAGCCTCTAGCTGAATCTCTCACCTTTCTTAGTCTCGATGACAACAACATCAGTGGCGGAATAACATCTGAGATCGGAAACTGCAAACAACTCACTCGCCTTCACCTAAGCGGAAACCAACTTGCTGGAAACCTTCCTACTTCACTTGCCATGTTGAACAATTTGAAAAGACTAGACATTTCAAACAACAAGTTTTCGGGACCGTTGCCAAACCTGGCTAGAATCTCAGGCCTCAATATGTTCCTAGCACAAAACAATGAACTTAGTGGAAATATACCAACATTTTATTTCTCTAATTTTGACCGTTTCAATGTTTCCTTTAACAATTTTAGTGGTCCAATTCCAGATTTGCAAGGTTATTTCTTTGCAGACAGTTTTCTTGGTAATCCAAGACTATGTGGATATCCACTACAAAAAAATTGTACTAGTCAACCTTTAAGTACTGTTAGTAAAATATCAGAACAAGAATCAAAGGGTGCTTCTAAAGAACAAATTCTTATGTACTCAGGCTATGCTGCATTAGGTTTTATCATAATCTTTTTTGTAGTCTGGAAGCTGTGTATAAGAAAGAAGAGTGGGAAAAAAGTTCAAGCAGAACTGAAAGATGATATCGAAAAACCGAGCTATATTTCAAGCGAATCCAAAGCAGAAGAGCTAAGCAAATCAGGATTTTCAGTTACCTCTGAGAGTGGATTGGTCTCACAACAATCACTTATTGTTCTTTCAAGACCAGTTGTGAGTGAATTGAAATTGGAAGAGTTGTTGAGAGCACCTGCTGAAATGATAGGAAGAGGAAAGAATGGAAGTCTTTATAAGGTGATGCTAACCAATGGGATTCAAGTGGTTGTAAAAAGGATTAAGGATTGGAGTATTTCTAGTGTGGAATTCAAGCAAAGAATGCAACTATTGAATCAAGCTAAGCACCCTCATGTACTTTCACCTCTTGCTTTCTATTGTTCCAAACAAGAGAAGCTTTTGGTCTATGAATATCAGCAGAATGGAAGTTTGTTCAAGCTTCTACATGGCACATCAAAAACCTTTGATTGGAGCAGCAGACTTGGAATTGCATCTACAATTGCTGAAGCATTAGCTTTCATGCATCAAGAGCTTGGCCAACATGGTATTATTCATGGTAACATGAAATCCTCAAACATTTTGCTAAACATTAACATGGAACCTTGTATTAGTGAATATGGCACTATGCCAAAGGATGATCAACAAAGTTCAACTTTTGCAACTCCTAATAATGCTAATGCATTCAAGGAAGATGTTTATGGATTTGGTGTTATTCTTCTTGAACTACTGACTGGAAAATTGGTGAAGAGTAATGGTATTGACTTGACTGAATGGGTTCAATCAGTTGTAAGGGAAGAATGGACAGGTGAAGTATTTGATAGGTCATTATTGAAAGAGTATGCAAGTGAAGAGAGGATGGTTAATATGCTTCAAGTGGCTATAAGATGTATCAATAGTTCATTAGAGGCTAGGCCAACTATGAATCAAGTGGCTCTTATGATTAACACTATCAAAGATGAAGAGGAAAAATCCTTGATCTACCAAGTTTGA

Protein sequence

>Msa0316060

MEIISNILLVFLVTPFFLLNMISCENDEMKRTLIQFLTQLKGQQNDNSTLVWKPDTDPCKDHWNGVYCDAQMSIKKLDLYRFNLSGTLDVALLCNLQPLAESLTFLSLDDNNISGGITSEIGNCKQLTRLHLSGNQLAGNLPTSLAMLNNLKRLDISNNKFSGPLPNLARISGLNMFLAQNNELSGNIPTFYFSNFDRFNVSFNNFSGPIPDLQGYFFADSFLGNPRLCGYPLQKNCTSQPLSTVSKISEQESKGASKEQILMYSGYAALGFIIIFFVVWKLCIRKKSGKKVQAELKDDIEKPSYISSESKAEELSKSGFSVTSESGLVSQQSLIVLSRPVVSELKLEELLRAPAEMIGRGKNGSLYKVMLTNGIQVVVKRIKDWSISSVEFKQRMQLLNQAKHPHVLSPLAFYCSKQEKLLVYEYQQNGSLFKLLHGTSKTFDWSSRLGIASTIAEALAFMHQELGQHGIIHGNMKSSNILLNINMEPCISEYGTMPKDDQQSSTFATPNNANAFKEDVYGFGVILLELLTGKLVKSNGIDLTEWVQSVVREEWTGEVFDRSLLKEYASEERMVNMLQVAIRCINSSLEARPTMNQVALMINTIKDEEEKSLIYQV*