AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0317230

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0317230 MtrunA17_Chr2g0290391 94.118 697 39 1 1 697 1 695 0.0 1377
Msa0317230 MtrunA17_Chr1g0198761 46.044 632 308 6 54 668 48 663 0.0 560
Msa0317230 MtrunA17_Chr2g0276841 43.109 624 335 5 53 668 70 681 0.0 537
Msa0317230 MtrunA17_Chr2g0294841 39.510 653 372 5 27 668 21 661 1.53e-166 496
Msa0317230 MtrunA17_Chr5g0431001 44.548 431 222 3 52 475 60 480 1.93e-129 392
Msa0317230 MtrunA17_Chr5g0448761 30.229 655 412 16 51 678 58 694 4.11e-75 257
Msa0317230 MtrunA17_Chr1g0202341 30.382 576 369 11 51 612 49 606 2.80e-72 248
Msa0317230 MtrunA17_Chr5g0440451 26.395 663 415 18 53 677 43 670 2.34e-59 216
Msa0317230 MtrunA17_Chr6g0458391 27.911 584 353 13 53 613 225 763 2.90e-57 207
Msa0317230 MtrunA17_Chr6g0458391 32.031 128 77 4 51 175 89 209 5.93e-11 65.9
Msa0317230 MtrunA17_Chr2g0287651 25.308 569 390 11 59 612 81 629 5.67e-57 208
Msa0317230 MtrunA17_Chr3g0082121 28.007 607 377 16 32 616 23 591 6.74e-57 205
Msa0317230 MtrunA17_Chr5g0441581 27.273 616 389 15 30 623 9 587 1.42e-56 204
Msa0317230 MtrunA17_Chr1g0203051 25.494 557 382 10 59 602 85 621 5.73e-55 199
Msa0317230 MtrunA17_Chr1g0207551 27.350 585 376 17 53 616 34 590 7.37e-55 201
Msa0317230 MtrunA17_Chr8g0363841 27.474 586 369 15 54 612 18 574 1.10e-53 196
Msa0317230 MtrunA17_Chr2g0329031 27.521 585 361 14 50 610 11 556 1.58e-53 196
Msa0317230 MtrunA17_Chr3g0080351 25.240 626 406 18 51 652 74 661 5.36e-53 196
Msa0317230 MtrunA17_Chr2g0287061 27.073 591 341 18 51 620 181 702 1.30e-52 194
Msa0317230 MtrunA17_Chr2g0324011 23.793 580 410 7 53 618 10 571 1.13e-48 180
Msa0317230 MtrunA17_Chr3g0118611 29.829 409 272 7 240 636 118 523 2.13e-48 182
Msa0317230 MtrunA17_Chr2g0296781 24.658 584 383 17 57 613 1 554 6.19e-46 174
Msa0317230 MtrunA17_Chr5g0448511 28.715 397 266 8 243 625 15 408 4.74e-45 169
Msa0317230 MtrunA17_Chr6g0477261 26.681 461 295 13 177 613 2 443 1.02e-40 158
Msa0317230 MtrunA17_Chr2g0291581 25.671 596 383 17 52 623 70 629 1.08e-40 159
Msa0317230 MtrunA17_Chr1g0203721 23.102 619 419 16 30 616 31 624 2.41e-39 155
Msa0317230 MtrunA17_Chr1g0196541 24.196 591 375 16 57 612 1 553 2.85e-38 152
Msa0317230 MtrunA17_Chr2g0291591 24.276 449 315 10 41 486 52 478 7.16e-33 134
Msa0317230 MtrunA17_Chr1g0164591 26.791 321 214 5 141 460 9 309 6.01e-30 121
Msa0317230 MtrunA17_Chr3g0081281 44.660 103 47 1 194 296 1 93 2.65e-23 94.7
Msa0317230 MtrunA17_Chr7g0258861 25.753 299 195 8 53 343 10 289 3.14e-22 98.2
Msa0317230 MtrunA17_Chr7g0233911 33.835 133 82 3 28 157 7 136 4.96e-13 68.9
Msa0317230 MtrunA17_Chr2g0331371 38.235 102 60 2 65 165 67 166 1.35e-11 64.7
Msa0317230 MtrunA17_Chr2g0308841 25.234 214 139 6 415 612 16 224 5.11e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0317230 AT5G28530.3 56.607 666 287 2 5 670 8 671 0.0 785
Msa0317230 AT5G28530.2 56.607 666 287 2 5 670 8 671 0.0 785
Msa0317230 AT5G28530.1 56.418 670 286 3 5 670 8 675 0.0 781
Msa0317230 AT5G28530.4 49.851 670 267 5 5 670 8 612 0.0 650
Msa0317230 AT5G28530.5 48.198 666 280 5 5 670 8 608 0.0 628
Msa0317230 AT1G10240.2 42.256 665 355 8 30 678 24 675 0.0 543
Msa0317230 AT1G10240.1 42.256 665 355 8 30 678 24 675 0.0 543
Msa0317230 AT4G38180.1 30.912 592 379 12 51 627 71 647 1.31e-71 248
Msa0317230 AT4G15090.1 26.604 639 418 15 51 665 49 660 1.53e-61 221
Msa0317230 AT4G15090.5 26.604 639 418 15 51 665 49 660 1.53e-61 221
Msa0317230 AT4G15090.3 26.604 639 418 15 51 665 49 660 1.53e-61 221
Msa0317230 AT4G15090.2 26.604 639 418 15 51 665 49 660 1.53e-61 221
Msa0317230 AT4G15090.4 26.604 639 418 15 51 665 49 660 1.53e-61 221
Msa0317230 AT2G27110.2 27.464 619 373 18 30 622 19 587 3.89e-57 209
Msa0317230 AT2G27110.1 27.464 619 373 18 30 622 19 587 3.89e-57 209
Msa0317230 AT3G06250.2 26.504 615 355 18 27 612 155 701 1.50e-51 191
Msa0317230 AT3G06250.1 26.504 615 355 18 27 612 155 701 1.50e-51 191
Msa0317230 AT3G06250.3 26.504 615 355 18 27 612 155 701 1.50e-51 191
Msa0317230 AT2G27110.3 30.049 406 261 9 234 622 43 442 1.37e-49 185
Msa0317230 AT1G76320.3 26.254 678 424 22 57 696 1 640 1.39e-49 184
Msa0317230 AT1G76320.2 25.991 681 422 22 57 696 1 640 4.57e-49 184
Msa0317230 AT1G76320.1 25.991 681 422 22 57 696 1 640 6.51e-49 184
Msa0317230 AT1G76320.4 25.991 681 422 22 57 696 1 640 6.51e-49 184
Msa0317230 AT5G18960.2 27.090 598 358 19 31 612 190 725 7.43e-49 184
Msa0317230 AT5G18960.1 27.090 598 358 19 31 612 190 725 7.43e-49 184
Msa0317230 AT3G22170.2 24.872 587 379 16 51 613 68 616 1.95e-48 183
Msa0317230 AT3G22170.1 24.872 587 379 16 51 613 68 616 1.95e-48 183
Msa0317230 AT1G52520.1 25.077 646 421 22 52 668 84 695 3.45e-42 164
Msa0317230 AT1G80010.1 23.767 669 437 20 28 657 72 706 2.11e-39 155
Msa0317230 AT2G32250.6 25.835 449 304 11 258 685 187 627 1.47e-38 153
Msa0317230 AT2G32250.1 25.835 449 304 11 258 685 187 627 1.47e-38 153
Msa0317230 AT2G32250.3 25.835 449 304 11 258 685 187 627 1.47e-38 153
Msa0317230 AT2G32250.5 25.835 449 304 11 258 685 187 627 1.49e-38 153
Msa0317230 AT2G32250.2 25.835 449 304 11 258 685 187 627 1.64e-38 153
Msa0317230 AT2G32250.4 25.835 449 304 11 258 685 187 627 1.64e-38 153
Msa0317230 AT2G32250.8 25.835 449 304 11 258 685 146 586 2.13e-38 152
Msa0317230 AT2G32250.7 25.835 449 304 11 258 685 146 586 2.42e-38 152
Msa0317230 AT4G19990.7 24.302 609 376 14 56 632 38 593 2.07e-37 150
Msa0317230 AT4G19990.3 24.302 609 376 14 56 632 24 579 2.27e-37 149
Msa0317230 AT4G19990.4 24.302 609 376 14 56 632 24 579 2.27e-37 149
Msa0317230 AT4G19990.6 24.532 587 361 13 56 613 24 557 2.63e-37 149
Msa0317230 AT4G19990.2 24.532 587 361 13 56 613 24 557 2.63e-37 149
Msa0317230 AT4G19990.5 24.302 609 376 14 56 632 24 579 2.66e-37 149
Msa0317230 AT4G38170.1 26.171 363 255 6 272 622 45 406 1.71e-33 135
Msa0317230 AT4G19990.1 24.165 509 321 10 119 613 43 500 6.57e-33 135

Find 158 sgRNAs with CRISPR-Local

Find 235 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCCAAATGGTTTCTGATTT+TGG 0.102866 2_4:-14870774 Msa0317230:CDS
AAATTGTTGAATGTTTATTT+TGG 0.125158 2_4:+14872490 None:intergenic
AAGAAGGGTTTATATGTTTC+TGG 0.141357 2_4:-14872378 Msa0317230:intron
CAGAGGAATTTGAACTTAAA+TGG 0.181226 2_4:-14870797 Msa0317230:CDS
TTATCTGCTTTCACCGATTC+TGG 0.206477 2_4:+14872014 None:intergenic
TCTTATCGATCGATTACTTA+TGG 0.224783 2_4:-14871234 Msa0317230:CDS
AGGTGGACATTTGTCTTTCT+TGG 0.238238 2_4:-14871508 Msa0317230:CDS
CCATTTGCCTTCCTCTTAAC+CGG 0.251085 2_4:+14871801 None:intergenic
AAGCCTTCAAACTAGCTATT+TGG 0.254902 2_4:+14869512 None:intergenic
CGTGCATAATCACGAGCTTT+TGG 0.255977 2_4:-14871667 Msa0317230:CDS
ACATATTGATTTACTGTATT+CGG 0.284863 2_4:-14870736 Msa0317230:CDS
TGTATTCGGTTCGAGCATAT+TGG 0.298238 2_4:-14870722 Msa0317230:CDS
GAAAAGGGAAAATGATGTTT+TGG 0.322914 2_4:-14871415 Msa0317230:CDS
GCTTTCGAAAGCTGGGTTTC+CGG 0.342293 2_4:-14871574 Msa0317230:CDS
AAATCGGAAGAAGGTTATTC+AGG 0.354212 2_4:-14871460 Msa0317230:CDS
ATACTCTTGCTTTCGAAAGC+TGG 0.354980 2_4:-14871582 Msa0317230:CDS
TTCTTTCATCAGTTCTTTGC+GGG 0.358476 2_4:+14869625 None:intergenic
ATTGAATGTGGATTTAAATT+CGG 0.362006 2_4:+14870842 None:intergenic
TGCAAGGAATTTGAATCATC+GGG 0.363156 2_4:-14869869 Msa0317230:CDS
TACCAAACCACACTCCAAAA+AGG 0.366681 2_4:+14871208 None:intergenic
GTATTCGGTTCGAGCATATT+GGG 0.368888 2_4:-14870721 Msa0317230:CDS
CTCAGGAAATTCACTTCTAA+TGG 0.369848 2_4:+14870944 None:intergenic
AACAATGTCCTTGTGGAGAT+TGG 0.375018 2_4:-14872088 Msa0317230:CDS
AAATAGCTAGTTTGAAGGCT+TGG 0.379213 2_4:-14869510 Msa0317230:exon
ATCCTAGATTTGTTATCATC+AGG 0.386662 2_4:-14869368 Msa0317230:three_prime_UTR
TAAAGTTTATTAAATGTTCA+AGG 0.389495 2_4:-14869338 Msa0317230:three_prime_UTR
CCAGTGAATTTGCAAGGAAC+CGG 0.392417 2_4:-14872438 Msa0317230:five_prime_UTR
ATCAATTTCTCAAAATTTCA+AGG 0.394834 2_4:+14872568 None:intergenic
TTAAACACCATTCCAACATA+AGG 0.399971 2_4:+14871963 None:intergenic
TCTAGAATTAGCCCTCAATT+AGG 0.402768 2_4:-14871867 Msa0317230:CDS
GTCATCATCTGCTTCTTTCG+TGG 0.402884 2_4:+14871371 None:intergenic
TGAGGAACTTTGTGCAAAAT+CGG 0.408597 2_4:-14871476 Msa0317230:CDS
AGGCATTGCTTAATGAGAAA+AGG 0.415782 2_4:-14871431 Msa0317230:CDS
TCTAATGGCATCTTTAAGTC+CGG 0.417527 2_4:+14870959 None:intergenic
TAAACACCATTCCAACATAA+GGG 0.418566 2_4:+14871964 None:intergenic
CCGGGTTTGCCCCGGTTAAG+AGG 0.420690 2_4:-14871812 Msa0317230:CDS
GAAAATATAGCTTGGTCATA+TGG 0.423296 2_4:-14871309 Msa0317230:CDS
CCACGTTTAAAGATCGTTCT+CGG 0.425296 2_4:+14869762 None:intergenic
GACATCTTCCTTTCATGAAC+CGG 0.427391 2_4:+14871009 None:intergenic
GAAAATGCTTGAGCTTGAAA+AGG 0.428355 2_4:-14871538 Msa0317230:CDS
GTCCAAAATCAGAAACCATT+TGG 0.428679 2_4:+14870772 None:intergenic
ATTTCTTAGCAATAAAACTT+TGG 0.431448 2_4:+14869723 None:intergenic
CGGTTAAGAGGAAGGCAAAT+GGG 0.435855 2_4:-14871800 Msa0317230:CDS
TGCTAAAATGTATCTGTCTA+AGG 0.437024 2_4:-14871736 Msa0317230:CDS
ACAAATACTACTTAAGTAGA+TGG 0.437071 2_4:-14869787 Msa0317230:CDS
AGTGGCAAAGTATAGATTGC+AGG 0.437433 2_4:-14869429 Msa0317230:three_prime_UTR
TACTCTTGCTTTCGAAAGCT+GGG 0.437822 2_4:-14871581 Msa0317230:CDS
ATGGAGAGCGACACGTGTTA+TGG 0.440990 2_4:-14869922 Msa0317230:CDS
TCACCAAATAGCTAGTTTGA+AGG 0.443693 2_4:-14869515 Msa0317230:exon
GTTAATAAGAATGTCGTGTT+TGG 0.444083 2_4:-14871279 Msa0317230:CDS
GAGCTTGAAAAGGGAATTCA+AGG 0.444538 2_4:-14871528 Msa0317230:CDS
CTACTTAAGTAGTATTTGTC+GGG 0.448293 2_4:+14869791 None:intergenic
CATTTGCCTTCCTCTTAACC+GGG 0.453188 2_4:+14871802 None:intergenic
CTTCAAAAGACGTATGGATT+CGG 0.455725 2_4:-14872115 Msa0317230:CDS
ACATTTGTCTTTCTTGGAAA+GGG 0.456261 2_4:-14871502 Msa0317230:CDS
CGTGTTATGGCTACCAGAAA+AGG 0.458295 2_4:-14869909 Msa0317230:CDS
CTCCGCGCATGCTCTTCAAT+TGG 0.460247 2_4:+14870061 None:intergenic
AACCTTCTCATTCTCATCCA+TGG 0.461287 2_4:+14871332 None:intergenic
CAATGTACAAATCTTAGAAC+TGG 0.461973 2_4:-14870088 Msa0317230:CDS
GTTCTTTCATCAGTTCTTTG+CGG 0.462201 2_4:+14869624 None:intergenic
CTAATGGCATCTTTAAGTCC+GGG 0.464686 2_4:+14870960 None:intergenic
CCTCGATGCAGGTGTTTCTC+CGG 0.467695 2_4:-14871028 Msa0317230:intron
TTCTAAACAAGGTATCTAGT+TGG 0.469074 2_4:-14870893 Msa0317230:CDS
ATGGGGAGCATCATAGAGAT+AGG 0.469186 2_4:-14871782 Msa0317230:CDS
CATAAAAGCATCTATAGACT+TGG 0.476239 2_4:+14870644 None:intergenic
GGCATTGCTTAATGAGAAAA+GGG 0.476407 2_4:-14871430 Msa0317230:CDS
CCGGTTCCTTGCAAATTCAC+TGG 0.476432 2_4:+14872438 None:intergenic
TTATCGATCCGGGTTTGCCC+CGG 0.478798 2_4:-14871820 Msa0317230:CDS
AGGTTCTGCAGCACCAGAAT+CGG 0.478841 2_4:-14872027 Msa0317230:CDS
AGCCTGATGATAACAAATCT+AGG 0.479822 2_4:+14869366 None:intergenic
CTGTCTAAGGAGGTTGTTGA+CGG 0.480756 2_4:-14871723 Msa0317230:CDS
GATATCATTACCCCTTATGT+TGG 0.483803 2_4:-14871975 Msa0317230:CDS
CCTCTTAACCGGGGCAAACC+CGG 0.485059 2_4:+14871812 None:intergenic
CCGGTTAAGAGGAAGGCAAA+TGG 0.486458 2_4:-14871801 Msa0317230:CDS
TCTACTTAAGTAGTATTTGT+CGG 0.487675 2_4:+14869790 None:intergenic
AAAATCACTGGATGCTCTTG+AGG 0.488028 2_4:-14872404 Msa0317230:five_prime_UTR
GACATTTGTCTTTCTTGGAA+AGG 0.490686 2_4:-14871503 Msa0317230:CDS
TGTCTAAGGAGGTTGTTGAC+GGG 0.496899 2_4:-14871722 Msa0317230:CDS
TGAGACTAAACACGTCATTC+CGG 0.497303 2_4:-14870925 Msa0317230:CDS
TCTAGATCTTGCTTTCCTCA+TGG 0.497710 2_4:+14871884 None:intergenic
CGCTTGTAAACGCCTAATTG+AGG 0.499806 2_4:+14871855 None:intergenic
TTGCAAGGAATTTGAATCAT+CGG 0.500581 2_4:-14869870 Msa0317230:CDS
TCATGAATCTTCCGATAAGC+AGG 0.501312 2_4:+14871621 None:intergenic
AATTTCAAGGAAATAACTAC+AGG 0.503798 2_4:+14872581 None:intergenic
TGAACTTAAATGGAGCCAAA+TGG 0.503893 2_4:-14870787 Msa0317230:CDS
AAAATGCTTGAGCTTGAAAA+GGG 0.510249 2_4:-14871537 Msa0317230:CDS
TGCAAAGCATCAAAAGCATA+AGG 0.512262 2_4:+14870028 None:intergenic
TGATGCTTTGCAGCAAGAGT+TGG 0.512354 2_4:-14870017 Msa0317230:CDS
CATTACCCCTTATGTTGGAA+TGG 0.512394 2_4:-14871970 Msa0317230:CDS
CTTTGTGCAAAATCGGAAGA+AGG 0.512580 2_4:-14871469 Msa0317230:CDS
AGGTCAGATAGAATTGTTTG+TGG 0.513189 2_4:+14870987 None:intergenic
AATCATCGGGAATATTATGC+AGG 0.520423 2_4:-14869856 Msa0317230:CDS
GCCGTTAATCACAAAATCAC+TGG 0.522373 2_4:-14872416 Msa0317230:five_prime_UTR
CAATGGCTTCAAAAGACGTA+TGG 0.527901 2_4:-14872121 Msa0317230:CDS
CATCTTTAAGTCCGGGGTCA+AGG 0.529096 2_4:+14870967 None:intergenic
GTTTGCCCCGGTTAAGAGGA+AGG 0.530147 2_4:-14871808 Msa0317230:CDS
GCTTGTAAACGCCTAATTGA+GGG 0.531080 2_4:+14871856 None:intergenic
GGAATGACGTGTTTAGTCTC+AGG 0.531762 2_4:+14870927 None:intergenic
TATCATCATAATATAATAAG+TGG 0.531821 2_4:-14869447 Msa0317230:three_prime_UTR
GAGGGTGATGATTCTGATGA+AGG 0.535697 2_4:-14872047 Msa0317230:CDS
GATGCTCTTGAGGAAGAAGA+AGG 0.535814 2_4:-14872394 Msa0317230:five_prime_UTR
TTCAGAATCATCAATTACAA+AGG 0.545164 2_4:-14869663 Msa0317230:CDS
GTGTTTCTCCGGTTCATGAA+AGG 0.545182 2_4:-14871017 Msa0317230:CDS
ACTTACTAGAATTCTTAATG+AGG 0.548103 2_4:-14869603 Msa0317230:CDS
AAATGCAACTTGGAAACCAC+TGG 0.548182 2_4:-14869470 Msa0317230:three_prime_UTR
GCTACATCTGAGCTGTATAA+CGG 0.548265 2_4:-14869977 Msa0317230:CDS
GATAGGAAATCAGTGAGGTG+TGG 0.551495 2_4:-14871765 Msa0317230:CDS
ACCGATGGGGTCGAGACCGA+TGG 0.554843 2_4:-14869563 Msa0317230:CDS
GAGATATGTCTAAGACCGAT+GGG 0.555361 2_4:-14869577 Msa0317230:CDS
GCGTTTCCAGTGAATTTGCA+AGG 0.555592 2_4:-14872444 Msa0317230:five_prime_UTR
AGAAGGTTGAAAATATAGCT+TGG 0.556814 2_4:-14871317 Msa0317230:CDS
AGAGATATGTCTAAGACCGA+TGG 0.562467 2_4:-14869578 Msa0317230:CDS
AAATAACCTCTCACATAGGA+TGG 0.565070 2_4:+14870696 None:intergenic
GAAGGTTATTCAGGAGAATG+AGG 0.565443 2_4:-14871451 Msa0317230:CDS
ATTCATGCTGCAAACTCCAT+CGG 0.569005 2_4:+14869547 None:intergenic
TTATATTATGATGATACCAG+TGG 0.573079 2_4:+14869454 None:intergenic
ATTCTATCTGACCTTGACCC+CGG 0.577694 2_4:-14870978 Msa0317230:CDS
ACAAATACTCTGCTCTTGCA+AGG 0.578215 2_4:-14869885 Msa0317230:CDS
TACCATGGATGAGAATGAGA+AGG 0.581496 2_4:-14871334 Msa0317230:CDS
ATATGTGATAAAATGCAACT+TGG 0.584427 2_4:-14869480 Msa0317230:three_prime_UTR
TCAAGCAGTGTAACAAACAA+TGG 0.589720 2_4:-14872138 Msa0317230:intron
CTTTCTTGGAAAGGGATGTG+AGG 0.596394 2_4:-14871494 Msa0317230:CDS
TGAGTCTCCTTATGTGATTG+AGG 0.600028 2_4:+14870109 None:intergenic
GAGGAATAAGGCATTGAATG+TGG 0.601090 2_4:+14870830 None:intergenic
TTCAAGGAAATAACTACAGG+GGG 0.603409 2_4:+14872584 None:intergenic
TACCAATTGAAGAGCATGCG+CGG 0.607691 2_4:-14870063 Msa0317230:CDS
TAATGGCATCTTTAAGTCCG+GGG 0.609613 2_4:+14870961 None:intergenic
GATTTAAATTCGGCATAACG+AGG 0.612578 2_4:+14870852 None:intergenic
GTCTAAGGAGGTTGTTGACG+GGG 0.619389 2_4:-14871721 Msa0317230:CDS
AAACACCATTCCAACATAAG+GGG 0.620023 2_4:+14871965 None:intergenic
CGGAAACAACAATGTCCTTG+TGG 0.620981 2_4:-14872095 Msa0317230:CDS
AAGCTGGGTTTCCGGTACAC+CGG 0.621789 2_4:-14871566 Msa0317230:CDS
GGTTAAGAGGAAGGCAAATG+GGG 0.628905 2_4:-14871799 Msa0317230:CDS
TCGGCATAACGAGGCCCAAG+AGG 0.630511 2_4:+14870861 None:intergenic
TGGTTTGGTATCAATAGTTG+TGG 0.630835 2_4:-14871195 Msa0317230:CDS
ATGCTCTTGAGGAAGAAGAA+GGG 0.631598 2_4:-14872393 Msa0317230:five_prime_UTR
TCCATCGGTCTCGACCCCAT+CGG 0.633244 2_4:+14869562 None:intergenic
ATTTCAAGGAAATAACTACA+GGG 0.637231 2_4:+14872582 None:intergenic
TGGAGTTTGCAGCATGAATG+TGG 0.649735 2_4:-14869543 Msa0317230:CDS
TAAAATGTATCTGTCTAAGG+AGG 0.650992 2_4:-14871733 Msa0317230:CDS
TTGTTGACGGGGTTTCACAG+TGG 0.656963 2_4:-14871710 Msa0317230:CDS
ATTTGCCTTCCTCTTAACCG+GGG 0.662005 2_4:+14871803 None:intergenic
TGGGCACCATCCTATGTGAG+AGG 0.663195 2_4:-14870702 Msa0317230:CDS
TTTCAAGGAAATAACTACAG+GGG 0.663821 2_4:+14872583 None:intergenic
TTGTTTAGAATATTCCACAC+CGG 0.665129 2_4:+14870906 None:intergenic
CCGAGAACGATCTTTAAACG+TGG 0.667057 2_4:-14869762 Msa0317230:CDS
GGTGTGGAATATTCTAAACA+AGG 0.670977 2_4:-14870904 Msa0317230:CDS
AATGCCCATGAAAATGATTG+TGG 0.675484 2_4:+14871129 None:intergenic
TGCTTATCGGAAGATTCATG+AGG 0.681046 2_4:-14871619 Msa0317230:CDS
AGAACGATCTTTAAACGTGG+AGG 0.689325 2_4:-14869759 Msa0317230:CDS
CTTGAAAAGGGAATTCAAGG+TGG 0.689739 2_4:-14871525 Msa0317230:CDS
AAGTGTTACATTACAAACGA+GGG 0.690032 2_4:-14872065 Msa0317230:CDS
ATTCCTCATCGAGAAAACAG+AGG 0.705269 2_4:-14870814 Msa0317230:CDS
TAACACTTCCAATCTCCACA+AGG 0.707021 2_4:+14872080 None:intergenic
GAAGTGTTACATTACAAACG+AGG 0.716498 2_4:-14872066 Msa0317230:CDS
CTAAACACGTCATTCCGGTG+TGG 0.723440 2_4:-14870920 Msa0317230:CDS
AGATATGTCTAAGACCGATG+GGG 0.747503 2_4:-14869576 Msa0317230:CDS
ATAGAGATAGGAAATCAGTG+AGG 0.812534 2_4:-14871770 Msa0317230:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTTATATCAAGAAAAAAT+GGG + chr2_4:14869609-14869628 None:intergenic 10.0%
!! TTATTTATATCAAGAAAAAA+TGG + chr2_4:14869610-14869629 None:intergenic 10.0%
!!! ATTTTGTTTAATTCATATTA+AGG - chr2_4:14869666-14869685 Msa0317230:CDS 10.0%
!!! TTTTGTTTAATTCATATTAA+GGG - chr2_4:14869667-14869686 Msa0317230:CDS 10.0%
!! AAAACCCAAAAAATATTAAA+CGG - chr2_4:14869366-14869385 Msa0317230:three_prime_UTR 15.0%
!!! AATTGTTGAATGTTTATTTT+GGG + chr2_4:14869408-14869427 None:intergenic 15.0%
!!! AAATTGTTGAATGTTTATTT+TGG + chr2_4:14869409-14869428 None:intergenic 15.0%
!! TAATTCACAATTCATAAAAT+AGG - chr2_4:14871647-14871666 Msa0317230:CDS 15.0%
!! TATCATCATAATATAATAAG+TGG - chr2_4:14872449-14872468 Msa0317230:five_prime_UTR 15.0%
!! TAAAGTTTATTAAATGTTCA+AGG - chr2_4:14872558-14872577 Msa0317230:five_prime_UTR 15.0%
!! ATCAATTTCTCAAAATTTCA+AGG + chr2_4:14869331-14869350 None:intergenic 20.0%
!!! TTGAAATTTTGAGAAATTGA+TGG - chr2_4:14869330-14869349 Msa0317230:three_prime_UTR 20.0%
!!! TGAAATTTTGAGAAATTGAT+GGG - chr2_4:14869331-14869350 Msa0317230:three_prime_UTR 20.0%
!!! TTAATTCATATTAAGGGTTT+TGG - chr2_4:14869673-14869692 Msa0317230:CDS 20.0%
!! ATTGAATGTGGATTTAAATT+CGG + chr2_4:14871057-14871076 None:intergenic 20.0%
!!! ACATATTGATTTACTGTATT+CGG - chr2_4:14871160-14871179 Msa0317230:CDS 20.0%
!! ATAATTCATTAGAATGCATT+GGG + chr2_4:14871465-14871484 None:intergenic 20.0%
!!! GTCATTTTTATTGTATCTTA+CGG - chr2_4:14871610-14871629 Msa0317230:CDS 20.0%
!! ATTTCTTAGCAATAAAACTT+TGG + chr2_4:14872176-14872195 None:intergenic 20.0%
!!! AAAGTTTTATTGCTAAGAAA+TGG - chr2_4:14872175-14872194 Msa0317230:intron 20.0%
!!! TGAAAATAAATTTTCAGCTT+GGG + chr2_4:14872217-14872236 None:intergenic 20.0%
! ATTTCAAGGAAATAACTACA+GGG + chr2_4:14869317-14869336 None:intergenic 25.0%
! AATTTCAAGGAAATAACTAC+AGG + chr2_4:14869318-14869337 None:intergenic 25.0%
!!! GAAATTTTGAGAAATTGATG+GGG - chr2_4:14869332-14869351 Msa0317230:three_prime_UTR 25.0%
!!! AAATTTTGAGAAATTGATGG+GGG - chr2_4:14869333-14869352 Msa0317230:three_prime_UTR 25.0%
!! TTTTGGGCTGTTTATTTATT+GGG + chr2_4:14869392-14869411 None:intergenic 25.0%
!! ATTTTGGGCTGTTTATTTAT+TGG + chr2_4:14869393-14869412 None:intergenic 25.0%
!!! TTTGGTTTTGTTGTTTCAAA+TGG - chr2_4:14869691-14869710 Msa0317230:CDS 25.0%
!!! TTGGTTTTGTTGTTTCAAAT+GGG - chr2_4:14869692-14869711 Msa0317230:CDS 25.0%
!!! CTTTGTTTATGATTTTACCA+TGG - chr2_4:14870547-14870566 Msa0317230:intron 25.0%
!! ATTACTTATGGATTCCTTTT+TGG - chr2_4:14870674-14870693 Msa0317230:CDS 25.0%
! TAAAACACAACCGAAAAAAA+TGG + chr2_4:14870732-14870751 None:intergenic 25.0%
! CATAAGAGGAATATTTATGA+AGG + chr2_4:14870839-14870858 None:intergenic 25.0%
!!! TCTTTGTTTTGTATGGTTTT+CGG - chr2_4:14871363-14871382 Msa0317230:CDS 25.0%
! CATAATTCATTAGAATGCAT+TGG + chr2_4:14871466-14871485 None:intergenic 25.0%
! AAAAGATTGACTATACAATG+TGG + chr2_4:14871532-14871551 None:intergenic 25.0%
! TCTACTTAAGTAGTATTTGT+CGG + chr2_4:14872109-14872128 None:intergenic 25.0%
! ACAAATACTACTTAAGTAGA+TGG - chr2_4:14872109-14872128 Msa0317230:CDS 25.0%
!! CTGAAAATAAATTTTCAGCT+TGG + chr2_4:14872218-14872237 None:intergenic 25.0%
! TTCAGAATCATCAATTACAA+AGG - chr2_4:14872233-14872252 Msa0317230:intron 25.0%
! ACTTACTAGAATTCTTAATG+AGG - chr2_4:14872293-14872312 Msa0317230:intron 25.0%
! ATATGTGATAAAATGCAACT+TGG - chr2_4:14872416-14872435 Msa0317230:five_prime_UTR 25.0%
! TTATATTATGATGATACCAG+TGG + chr2_4:14872445-14872464 None:intergenic 25.0%
TTTCAAGGAAATAACTACAG+GGG + chr2_4:14869316-14869335 None:intergenic 30.0%
!!! TGGGCCGTTTAATATTTTTT+GGG + chr2_4:14869373-14869392 None:intergenic 30.0%
!!! TTGGGCCGTTTAATATTTTT+TGG + chr2_4:14869374-14869393 None:intergenic 30.0%
! TCCAGTGATTTTGTGATTAA+CGG + chr2_4:14869484-14869503 None:intergenic 30.0%
AAGAAGGGTTTATATGTTTC+TGG - chr2_4:14869518-14869537 Msa0317230:exon 30.0%
ATGGGTTTATTGAATAAGCT+AGG + chr2_4:14869591-14869610 None:intergenic 30.0%
AAGTGTTACATTACAAACGA+GGG - chr2_4:14869831-14869850 Msa0317230:CDS 30.0%
TAAACACCATTCCAACATAA+GGG + chr2_4:14869935-14869954 None:intergenic 30.0%
TTAAACACCATTCCAACATA+AGG + chr2_4:14869936-14869955 None:intergenic 30.0%
!!! GATTTTGTTTGTTATCGATC+CGG - chr2_4:14870065-14870084 Msa0317230:CDS 30.0%
!!! ATTTTGTTTGTTATCGATCC+GGG - chr2_4:14870066-14870085 Msa0317230:CDS 30.0%
TGCTAAAATGTATCTGTCTA+AGG - chr2_4:14870160-14870179 Msa0317230:intron 30.0%
TAAAATGTATCTGTCTAAGG+AGG - chr2_4:14870163-14870182 Msa0317230:intron 30.0%
AAAATGCTTGAGCTTGAAAA+GGG - chr2_4:14870359-14870378 Msa0317230:intron 30.0%
ACATTTGTCTTTCTTGGAAA+GGG - chr2_4:14870394-14870413 Msa0317230:intron 30.0%
! GAAAAGGGAAAATGATGTTT+TGG - chr2_4:14870481-14870500 Msa0317230:intron 30.0%
AGAAGGTTGAAAATATAGCT+TGG - chr2_4:14870579-14870598 Msa0317230:intron 30.0%
GAAAATATAGCTTGGTCATA+TGG - chr2_4:14870587-14870606 Msa0317230:intron 30.0%
GTTAATAAGAATGTCGTGTT+TGG - chr2_4:14870617-14870636 Msa0317230:CDS 30.0%
TCTTATCGATCGATTACTTA+TGG - chr2_4:14870662-14870681 Msa0317230:CDS 30.0%
! AAACACCACAATCATTTTCA+TGG - chr2_4:14870762-14870781 Msa0317230:CDS 30.0%
! AACACCACAATCATTTTCAT+GGG - chr2_4:14870763-14870782 Msa0317230:CDS 30.0%
! TTGTTTAGAATATTCCACAC+CGG + chr2_4:14870993-14871012 None:intergenic 30.0%
! TTCTAAACAAGGTATCTAGT+TGG - chr2_4:14871003-14871022 Msa0317230:CDS 30.0%
CAGAGGAATTTGAACTTAAA+TGG - chr2_4:14871099-14871118 Msa0317230:intron 30.0%
CAAAAAATAACCTCTCACAT+AGG + chr2_4:14871207-14871226 None:intergenic 30.0%
!!! CTATGTGAGAGGTTATTTTT+TGG - chr2_4:14871205-14871224 Msa0317230:CDS 30.0%
!! CATAAAAGCATCTATAGACT+TGG + chr2_4:14871255-14871274 None:intergenic 30.0%
TATGAATCACCACTTAAACT+TGG - chr2_4:14871482-14871501 Msa0317230:CDS 30.0%
TAGGTATTCCATTTCAGATT+TGG - chr2_4:14871666-14871685 Msa0317230:CDS 30.0%
ATCAAGTTCCAAATCTGAAA+TGG + chr2_4:14871677-14871696 None:intergenic 30.0%
! TTTTCATCCTCAATCACATA+AGG - chr2_4:14871780-14871799 Msa0317230:CDS 30.0%
CAATGTACAAATCTTAGAAC+TGG - chr2_4:14871808-14871827 Msa0317230:CDS 30.0%
TTGCAAGGAATTTGAATCAT+CGG - chr2_4:14872026-14872045 Msa0317230:CDS 30.0%
CTACTTAAGTAGTATTTGTC+GGG + chr2_4:14872108-14872127 None:intergenic 30.0%
!! ATCCTAGATTTGTTATCATC+AGG - chr2_4:14872528-14872547 Msa0317230:five_prime_UTR 30.0%
TTCAAGGAAATAACTACAGG+GGG + chr2_4:14869315-14869334 None:intergenic 35.0%
TCAAGCAGTGTAACAAACAA+TGG - chr2_4:14869758-14869777 Msa0317230:CDS 35.0%
CTTCAAAAGACGTATGGATT+CGG - chr2_4:14869781-14869800 Msa0317230:CDS 35.0%
! GAAGTGTTACATTACAAACG+AGG - chr2_4:14869830-14869849 Msa0317230:CDS 35.0%
GATATCATTACCCCTTATGT+TGG - chr2_4:14869921-14869940 Msa0317230:CDS 35.0%
AAACACCATTCCAACATAAG+GGG + chr2_4:14869934-14869953 None:intergenic 35.0%
! GATGATGCTTTTGAGCATTA+TGG - chr2_4:14869963-14869982 Msa0317230:CDS 35.0%
! TCTAGAATTAGCCCTCAATT+AGG - chr2_4:14870029-14870048 Msa0317230:CDS 35.0%
ATAGAGATAGGAAATCAGTG+AGG - chr2_4:14870126-14870145 Msa0317230:CDS 35.0%
GAAAATGCTTGAGCTTGAAA+AGG - chr2_4:14870358-14870377 Msa0317230:intron 35.0%
GACATTTGTCTTTCTTGGAA+AGG - chr2_4:14870393-14870412 Msa0317230:intron 35.0%
TGAGGAACTTTGTGCAAAAT+CGG - chr2_4:14870420-14870439 Msa0317230:intron 35.0%
AAATCGGAAGAAGGTTATTC+AGG - chr2_4:14870436-14870455 Msa0317230:intron 35.0%
! AGGCATTGCTTAATGAGAAA+AGG - chr2_4:14870465-14870484 Msa0317230:intron 35.0%
GGCATTGCTTAATGAGAAAA+GGG - chr2_4:14870466-14870485 Msa0317230:intron 35.0%
!! TGGTTTGGTATCAATAGTTG+TGG - chr2_4:14870701-14870720 Msa0317230:CDS 35.0%
!!! TGTGGCAGAACCATTTTTTT+CGG - chr2_4:14870719-14870738 Msa0317230:CDS 35.0%
!! TTTCGGTTGTGTTTTATTGC+AGG - chr2_4:14870736-14870755 Msa0317230:CDS 35.0%
AATGCCCATGAAAATGATTG+TGG + chr2_4:14870770-14870789 None:intergenic 35.0%
! ATCATTTTCATGGGCATTAC+AGG - chr2_4:14870772-14870791 Msa0317230:CDS 35.0%
CGAGGAATAATCATCATAAG+AGG + chr2_4:14870853-14870872 None:intergenic 35.0%
AGGTCAGATAGAATTGTTTG+TGG + chr2_4:14870912-14870931 None:intergenic 35.0%
TCTAATGGCATCTTTAAGTC+CGG + chr2_4:14870940-14870959 None:intergenic 35.0%
CTCAGGAAATTCACTTCTAA+TGG + chr2_4:14870955-14870974 None:intergenic 35.0%
GGTGTGGAATATTCTAAACA+AGG - chr2_4:14870992-14871011 Msa0317230:CDS 35.0%
GATTTAAATTCGGCATAACG+AGG + chr2_4:14871047-14871066 None:intergenic 35.0%
! TGTTTTCTCGATGAGGAATA+AGG + chr2_4:14871081-14871100 None:intergenic 35.0%
TGAACTTAAATGGAGCCAAA+TGG - chr2_4:14871109-14871128 Msa0317230:intron 35.0%
! AGCCAAATGGTTTCTGATTT+TGG - chr2_4:14871122-14871141 Msa0317230:intron 35.0%
GTCCAAAATCAGAAACCATT+TGG + chr2_4:14871127-14871146 None:intergenic 35.0%
! ATGGTTTCTGATTTTGGACT+TGG - chr2_4:14871128-14871147 Msa0317230:CDS 35.0%
AAATAACCTCTCACATAGGA+TGG + chr2_4:14871203-14871222 None:intergenic 35.0%
!!! TTTGCGTAGCTTTTTTGAAC+AGG - chr2_4:14871301-14871320 Msa0317230:CDS 35.0%
!!! GCTTTTTTGAACAGGTATGT+TGG - chr2_4:14871309-14871328 Msa0317230:CDS 35.0%
!!! TGCTTGCTCTTTGTTTTGTA+TGG - chr2_4:14871356-14871375 Msa0317230:CDS 35.0%
ACAGAAATGTCATAAGAGCA+TGG + chr2_4:14871707-14871726 None:intergenic 35.0%
TACAACAGTATCCATGAGAA+GGG + chr2_4:14871742-14871761 None:intergenic 35.0%
! TGCAAAGCATCAAAAGCATA+AGG + chr2_4:14871871-14871890 None:intergenic 35.0%
TGTACGACACTTCAAAAAGT+TGG - chr2_4:14871951-14871970 Msa0317230:CDS 35.0%
!! CAGAGTATTTGTTCCTTTTC+TGG + chr2_4:14872003-14872022 None:intergenic 35.0%
TGCAAGGAATTTGAATCATC+GGG - chr2_4:14872027-14872046 Msa0317230:CDS 35.0%
AATCATCGGGAATATTATGC+AGG - chr2_4:14872040-14872059 Msa0317230:CDS 35.0%
TTCTTTCATCAGTTCTTTGC+GGG + chr2_4:14872274-14872293 None:intergenic 35.0%
GTTCTTTCATCAGTTCTTTG+CGG + chr2_4:14872275-14872294 None:intergenic 35.0%
! TCACCAAATAGCTAGTTTGA+AGG - chr2_4:14872381-14872400 Msa0317230:five_prime_UTR 35.0%
AAGCCTTCAAACTAGCTATT+TGG + chr2_4:14872387-14872406 None:intergenic 35.0%
! AAATAGCTAGTTTGAAGGCT+TGG - chr2_4:14872386-14872405 Msa0317230:five_prime_UTR 35.0%
AGCCTGATGATAACAAATCT+AGG + chr2_4:14872533-14872552 None:intergenic 35.0%
! GTGATTTTGTGATTAACGGC+CGG + chr2_4:14869480-14869499 None:intergenic 40.0%
GCCGTTAATCACAAAATCAC+TGG - chr2_4:14869480-14869499 Msa0317230:three_prime_UTR 40.0%
AAAATCACTGGATGCTCTTG+AGG - chr2_4:14869492-14869511 Msa0317230:three_prime_UTR 40.0%
ATGCTCTTGAGGAAGAAGAA+GGG - chr2_4:14869503-14869522 Msa0317230:three_prime_UTR 40.0%
CAATGGCTTCAAAAGACGTA+TGG - chr2_4:14869775-14869794 Msa0317230:CDS 40.0%
AACAATGTCCTTGTGGAGAT+TGG - chr2_4:14869808-14869827 Msa0317230:CDS 40.0%
TAACACTTCCAATCTCCACA+AGG + chr2_4:14869819-14869838 None:intergenic 40.0%
!! TTATCTGCTTTCACCGATTC+TGG + chr2_4:14869885-14869904 None:intergenic 40.0%
CATTACCCCTTATGTTGGAA+TGG - chr2_4:14869926-14869945 Msa0317230:CDS 40.0%
! GGCAATTTTGCTAGAAAGCA+TGG - chr2_4:14869984-14870003 Msa0317230:CDS 40.0%
!! GAAAGCATGGATTTTCCATG+AGG - chr2_4:14869997-14870016 Msa0317230:CDS 40.0%
TCTAGATCTTGCTTTCCTCA+TGG + chr2_4:14870015-14870034 None:intergenic 40.0%
GCTTGTAAACGCCTAATTGA+GGG + chr2_4:14870043-14870062 None:intergenic 40.0%
! TGCGTCTTTTACCTGCTTAT+CGG - chr2_4:14870264-14870283 Msa0317230:intron 40.0%
TCATGAATCTTCCGATAAGC+AGG + chr2_4:14870278-14870297 None:intergenic 40.0%
TGCTTATCGGAAGATTCATG+AGG - chr2_4:14870277-14870296 Msa0317230:intron 40.0%
ATACTCTTGCTTTCGAAAGC+TGG - chr2_4:14870314-14870333 Msa0317230:intron 40.0%
TACTCTTGCTTTCGAAAGCT+GGG - chr2_4:14870315-14870334 Msa0317230:intron 40.0%
! ATTTTCACTATCCGGTGTAC+CGG + chr2_4:14870344-14870363 None:intergenic 40.0%
! GCTCAAGCATTTTCACTATC+CGG + chr2_4:14870352-14870371 None:intergenic 40.0%
GAGCTTGAAAAGGGAATTCA+AGG - chr2_4:14870368-14870387 Msa0317230:intron 40.0%
CTTGAAAAGGGAATTCAAGG+TGG - chr2_4:14870371-14870390 Msa0317230:intron 40.0%
AGGTGGACATTTGTCTTTCT+TGG - chr2_4:14870388-14870407 Msa0317230:intron 40.0%
CTTTGTGCAAAATCGGAAGA+AGG - chr2_4:14870427-14870446 Msa0317230:intron 40.0%
GAAGGTTATTCAGGAGAATG+AGG - chr2_4:14870445-14870464 Msa0317230:intron 40.0%
TACCATGGATGAGAATGAGA+AGG - chr2_4:14870562-14870581 Msa0317230:intron 40.0%
AACCTTCTCATTCTCATCCA+TGG + chr2_4:14870567-14870586 None:intergenic 40.0%
!!! ATGGATTCCTTTTTGGAGTG+TGG - chr2_4:14870681-14870700 Msa0317230:CDS 40.0%
!!! TTCCTTTTTGGAGTGTGGTT+TGG - chr2_4:14870686-14870705 Msa0317230:CDS 40.0%
TACCAAACCACACTCCAAAA+AGG + chr2_4:14870691-14870710 None:intergenic 40.0%
TGATGATTATTCCTCGATGC+AGG - chr2_4:14870857-14870876 Msa0317230:CDS 40.0%
GACATCTTCCTTTCATGAAC+CGG + chr2_4:14870890-14870909 None:intergenic 40.0%
TAATGGCATCTTTAAGTCCG+GGG + chr2_4:14870938-14870957 None:intergenic 40.0%
CTAATGGCATCTTTAAGTCC+GGG + chr2_4:14870939-14870958 None:intergenic 40.0%
TGAGACTAAACACGTCATTC+CGG - chr2_4:14870971-14870990 Msa0317230:CDS 40.0%
!!! AGTTGGTTTTGTCTTCCTCT+TGG - chr2_4:14871020-14871039 Msa0317230:CDS 40.0%
!!! GTTGGTTTTGTCTTCCTCTT+GGG - chr2_4:14871021-14871040 Msa0317230:intron 40.0%
!! GAGGAATAAGGCATTGAATG+TGG + chr2_4:14871069-14871088 None:intergenic 40.0%
ATTCCTCATCGAGAAAACAG+AGG - chr2_4:14871082-14871101 Msa0317230:intron 40.0%
! ATTCCTCTGTTTTCTCGATG+AGG + chr2_4:14871088-14871107 None:intergenic 40.0%
! TGTATTCGGTTCGAGCATAT+TGG - chr2_4:14871174-14871193 Msa0317230:CDS 40.0%
! GTATTCGGTTCGAGCATATT+GGG - chr2_4:14871175-14871194 Msa0317230:CDS 40.0%
CAATGTGGACAACACAAAAG+TGG + chr2_4:14871517-14871536 None:intergenic 40.0%
! TTTCTGTTTCTCCCTTCTCA+TGG - chr2_4:14871728-14871747 Msa0317230:CDS 40.0%
CTACAACAGTATCCATGAGA+AGG + chr2_4:14871743-14871762 None:intergenic 40.0%
CTTCTCATGGATACTGTTGT+AGG - chr2_4:14871741-14871760 Msa0317230:CDS 40.0%
TCATGGATACTGTTGTAGGT+TGG - chr2_4:14871745-14871764 Msa0317230:CDS 40.0%
TGAGTCTCCTTATGTGATTG+AGG + chr2_4:14871790-14871809 None:intergenic 40.0%
GCTACATCTGAGCTGTATAA+CGG - chr2_4:14871919-14871938 Msa0317230:CDS 40.0%
CGACACTTCAAAAAGTTGGA+TGG - chr2_4:14871955-14871974 Msa0317230:CDS 40.0%
ACAAATACTCTGCTCTTGCA+AGG - chr2_4:14872011-14872030 Msa0317230:CDS 40.0%
CCACGTTTAAAGATCGTTCT+CGG + chr2_4:14872137-14872156 None:intergenic 40.0%
AGAACGATCTTTAAACGTGG+AGG - chr2_4:14872137-14872156 Msa0317230:intron 40.0%
! AGAGATATGTCTAAGACCGA+TGG - chr2_4:14872318-14872337 Msa0317230:intron 40.0%
! GAGATATGTCTAAGACCGAT+GGG - chr2_4:14872319-14872338 Msa0317230:intron 40.0%
! AGATATGTCTAAGACCGATG+GGG - chr2_4:14872320-14872339 Msa0317230:intron 40.0%
ATTCATGCTGCAAACTCCAT+CGG + chr2_4:14872352-14872371 None:intergenic 40.0%
AAATGCAACTTGGAAACCAC+TGG - chr2_4:14872426-14872445 Msa0317230:five_prime_UTR 40.0%
AGTGGCAAAGTATAGATTGC+AGG - chr2_4:14872467-14872486 Msa0317230:five_prime_UTR 40.0%
GCGTTTCCAGTGAATTTGCA+AGG - chr2_4:14869452-14869471 Msa0317230:three_prime_UTR 45.0%
CCAGTGAATTTGCAAGGAAC+CGG - chr2_4:14869458-14869477 Msa0317230:three_prime_UTR 45.0%
GATGCTCTTGAGGAAGAAGA+AGG - chr2_4:14869502-14869521 Msa0317230:three_prime_UTR 45.0%
CGGAAACAACAATGTCCTTG+TGG - chr2_4:14869801-14869820 Msa0317230:CDS 45.0%
GAGGGTGATGATTCTGATGA+AGG - chr2_4:14869849-14869868 Msa0317230:CDS 45.0%
CGCTTGTAAACGCCTAATTG+AGG + chr2_4:14870044-14870063 None:intergenic 45.0%
ATTTGCCTTCCTCTTAACCG+GGG + chr2_4:14870096-14870115 None:intergenic 45.0%
CATTTGCCTTCCTCTTAACC+GGG + chr2_4:14870097-14870116 None:intergenic 45.0%
CCATTTGCCTTCCTCTTAAC+CGG + chr2_4:14870098-14870117 None:intergenic 45.0%
CGGTTAAGAGGAAGGCAAAT+GGG - chr2_4:14870096-14870115 Msa0317230:CDS 45.0%
GGTTAAGAGGAAGGCAAATG+GGG - chr2_4:14870097-14870116 Msa0317230:CDS 45.0%
!! ATGGGGAGCATCATAGAGAT+AGG - chr2_4:14870114-14870133 Msa0317230:CDS 45.0%
GATAGGAAATCAGTGAGGTG+TGG - chr2_4:14870131-14870150 Msa0317230:CDS 45.0%
CTGTCTAAGGAGGTTGTTGA+CGG - chr2_4:14870173-14870192 Msa0317230:intron 45.0%
TGTCTAAGGAGGTTGTTGAC+GGG - chr2_4:14870174-14870193 Msa0317230:intron 45.0%
! CGTGCATAATCACGAGCTTT+TGG - chr2_4:14870229-14870248 Msa0317230:intron 45.0%
CTTTCTTGGAAAGGGATGTG+AGG - chr2_4:14870402-14870421 Msa0317230:intron 45.0%
GTCATCATCTGCTTCTTTCG+TGG + chr2_4:14870528-14870547 None:intergenic 45.0%
GTGTTTCTCCGGTTCATGAA+AGG - chr2_4:14870879-14870898 Msa0317230:CDS 45.0%
ATTCTATCTGACCTTGACCC+CGG - chr2_4:14870918-14870937 Msa0317230:CDS 45.0%
GGAATGACGTGTTTAGTCTC+AGG + chr2_4:14870972-14870991 None:intergenic 45.0%
TTGGCGTCACCAAGTTTAAG+TGG + chr2_4:14871494-14871513 None:intergenic 45.0%
GTGGACAACACAAAAGTGGT+TGG + chr2_4:14871513-14871532 None:intergenic 45.0%
! TACCAATTGAAGAGCATGCG+CGG - chr2_4:14871833-14871852 Msa0317230:CDS 45.0%
TGATGCTTTGCAGCAAGAGT+TGG - chr2_4:14871879-14871898 Msa0317230:CDS 45.0%
CGTGTTATGGCTACCAGAAA+AGG - chr2_4:14871987-14872006 Msa0317230:CDS 45.0%
CCGAGAACGATCTTTAAACG+TGG - chr2_4:14872134-14872153 Msa0317230:intron 45.0%
TGGAGTTTGCAGCATGAATG+TGG - chr2_4:14872353-14872372 Msa0317230:intron 45.0%
CCGGTTCCTTGCAAATTCAC+TGG + chr2_4:14869461-14869480 None:intergenic 50.0%
AGGTTCTGCAGCACCAGAAT+CGG - chr2_4:14869869-14869888 Msa0317230:CDS 50.0%
CCGGTTAAGAGGAAGGCAAA+TGG - chr2_4:14870095-14870114 Msa0317230:CDS 50.0%
GTCTAAGGAGGTTGTTGACG+GGG - chr2_4:14870175-14870194 Msa0317230:intron 50.0%
TTGTTGACGGGGTTTCACAG+TGG - chr2_4:14870186-14870205 Msa0317230:intron 50.0%
GCTTTCGAAAGCTGGGTTTC+CGG - chr2_4:14870322-14870341 Msa0317230:intron 50.0%
CATCTTTAAGTCCGGGGTCA+AGG + chr2_4:14870932-14870951 None:intergenic 50.0%
CTAAACACGTCATTCCGGTG+TGG - chr2_4:14870976-14870995 Msa0317230:CDS 50.0%
ATGGAGAGCGACACGTGTTA+TGG - chr2_4:14871974-14871993 Msa0317230:CDS 50.0%
TTATCGATCCGGGTTTGCCC+CGG - chr2_4:14870076-14870095 Msa0317230:CDS 55.0%
GTTTGCCCCGGTTAAGAGGA+AGG - chr2_4:14870088-14870107 Msa0317230:CDS 55.0%
AAGCTGGGTTTCCGGTACAC+CGG - chr2_4:14870330-14870349 Msa0317230:intron 55.0%
CCTCGATGCAGGTGTTTCTC+CGG - chr2_4:14870868-14870887 Msa0317230:CDS 55.0%
TGGGCACCATCCTATGTGAG+AGG - chr2_4:14871194-14871213 Msa0317230:CDS 55.0%
CTCCGCGCATGCTCTTCAAT+TGG + chr2_4:14871838-14871857 None:intergenic 55.0%
CCTCTTAACCGGGGCAAACC+CGG + chr2_4:14870087-14870106 None:intergenic 60.0%
CCGGAGAAACACCTGCATCG+AGG + chr2_4:14870871-14870890 None:intergenic 60.0%
TCGGCATAACGAGGCCCAAG+AGG + chr2_4:14871038-14871057 None:intergenic 60.0%
TCCATCGGTCTCGACCCCAT+CGG + chr2_4:14872337-14872356 None:intergenic 60.0%
CCGGGTTTGCCCCGGTTAAG+AGG - chr2_4:14870084-14870103 Msa0317230:CDS 65.0%
ACCGATGGGGTCGAGACCGA+TGG - chr2_4:14872333-14872352 Msa0317230:intron 65.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 14869301 14872617 14869301 ID=Msa0317230;Name=Msa0317230
chr2_4 mRNA 14869301 14872617 14869301 ID=Msa0317230-mRNA-1;Parent=Msa0317230;Name=Msa0317230-mRNA-1;_AED=0.00;_eAED=0.00;_QI=250|1|1|1|0.66|0.5|4|222|697
chr2_4 exon 14869301 14870155 14869301 ID=Msa0317230-mRNA-1:exon:5567;Parent=Msa0317230-mRNA-1
chr2_4 exon 14870596 14871039 14870596 ID=Msa0317230-mRNA-1:exon:5566;Parent=Msa0317230-mRNA-1
chr2_4 exon 14871125 14872152 14871125 ID=Msa0317230-mRNA-1:exon:5565;Parent=Msa0317230-mRNA-1
chr2_4 exon 14872379 14872617 14872379 ID=Msa0317230-mRNA-1:exon:5564;Parent=Msa0317230-mRNA-1
chr2_4 five_prime_UTR 14872379 14872617 14872379 ID=Msa0317230-mRNA-1:five_prime_utr;Parent=Msa0317230-mRNA-1
chr2_4 five_prime_UTR 14872142 14872152 14872142 ID=Msa0317230-mRNA-1:five_prime_utr;Parent=Msa0317230-mRNA-1
chr2_4 CDS 14871125 14872141 14871125 ID=Msa0317230-mRNA-1:cds;Parent=Msa0317230-mRNA-1
chr2_4 CDS 14870596 14871039 14870596 ID=Msa0317230-mRNA-1:cds;Parent=Msa0317230-mRNA-1
chr2_4 CDS 14869523 14870155 14869523 ID=Msa0317230-mRNA-1:cds;Parent=Msa0317230-mRNA-1
chr2_4 three_prime_UTR 14869301 14869522 14869301 ID=Msa0317230-mRNA-1:three_prime_utr;Parent=Msa0317230-mRNA-1
Gene Sequence

>Msa0317230

ATGGCTTCAAAAGACGTATGGATTCGGAAACAACAATGTCCTTGTGGAGATTGGAAGTGTTACATTACAAACGAGGGTGATGATTCTGATGAAGGTTCTGCAGCACCAGAATCGGTGAAAGCAGATAAAGATAAAACTCTGTCTGATATCATTACCCCTTATGTTGGAATGGTGTTTAAGAGTGATGATGATGCTTTTGAGCATTATGGCAATTTTGCTAGAAAGCATGGATTTTCCATGAGGAAAGCAAGATCTAGAATTAGCCCTCAATTAGGCGTTTACAAGCGTGATTTTGTTTGTTATCGATCCGGGTTTGCCCCGGTTAAGAGGAAGGCAAATGGGGAGCATCATAGAGATAGGAAATCAGTGAGGTGTGGATGTGATGCTAAAATGTATCTGTCTAAGGAGGTTGTTGACGGGGTTTCACAGTGGATTGTTCTGCAATTTAGTAACGTGCATAATCACGAGCTTTTGGAAGATGATCAAGTGCGTCTTTTACCTGCTTATCGGAAGATTCATGAGGCTGATCAAGAGCGTATACTCTTGCTTTCGAAAGCTGGGTTTCCGGTACACCGGATAGTGAAAATGCTTGAGCTTGAAAAGGGAATTCAAGGTGGACATTTGTCTTTCTTGGAAAGGGATGTGAGGAACTTTGTGCAAAATCGGAAGAAGGTTATTCAGGAGAATGAGGCATTGCTTAATGAGAAAAGGGAAAATGATGTTTTGGAACTTCTTGAGTTGTGCAAAGCCACGAAAGAAGCAGATGATGACTTTGTTTATGATTTTACCATGGATGAGAATGAGAAGGTTGAAAATATAGCTTGGTCATATGGTGATTCTGTTAATAAGAATGTCGTGTTTGGTGATGTAATTTATTTTGACACTTCTTATCGATCGATTACTTATGGATTCCTTTTTGGAGTGTGGTTTGGTATCAATAGTTGTGGCAGAACCATTTTTTTCGGTTGTGTTTTATTGCAGGACGAAACACCACAATCATTTTCATGGGCATTACAGGTGTTTCTCCGGTTCATGAAAGGAAGATGTCCACAAACAATTCTATCTGACCTTGACCCCGGACTTAAAGATGCCATTAGAAGTGAATTTCCTGAGACTAAACACGTCATTCCGGTGTGGAATATTCTAAACAAGGTATCTAGTTGGTTTTGTCTTCCTCTTGGGCCTCGTTATGCCGAATTTAAATCCACATTCAATGCCTTATTCCTCATCGAGAAAACAGAGGAATTTGAACTTAAATGGAGCCAAATGGTTTCTGATTTTGGACTTGGTTCAGATAAACATATTGATTTACTGTATTCGGTTCGAGCATATTGGGCACCATCCTATGTGAGAGGTTATTTTTTGGCTCGAATGTCAATAATAGCTTTTTCCAAGTCTATAGATGCTTTTATGAAAGAGATCGTCACTGAACATACATGTTTGCGTAGCTTTTTTGAACAGGTTGGTATTTCTGCAAATTTTCATCCTCAATCACATAAGGAGACTCAATGTACAAATCTTAGAACTGGCATACCAATTGAAGAGCATGCGCGGAGCATTCTCACGCCTTATGCTTTTGATGCTTTGCAGCAAGAGTTGGTGCTGACAATGCAATATGCTACATCTGAGCTGTATAACGGATCGTATATTGTACGACACTTCAAAAAGTTGGATGGAGAGCGACACGTGTTATGGCTACCAGAAAAGGAACAAATACTCTGCTCTTGCAAGGAATTTGAATCATCGGGAATATTATGCAGGCATGCTCTTCGTATACTTGTAACAAAGAATTACTTTCAGCTTCCCGACAAATACTACTTAAGTAGATGGCACCGAGAACGATCTTTAAACGTGGAGGATGAGCATAACAACCAAAGTTTTATTGCTAAGAAATGGTTTCAAGAATATCAGTCCCAAGCTGAAAATTTATTTTCAGAATCATCAATTACAAAGGAGCGATCTGACTATGCCCGCAAAGAACTGATGAAAGAACTTACTAGAATTCTTAATGAGGTTAGAGATATGTCTAAGACCGATGGGGTCGAGACCGATGGAGTTTGCAGCATGAATGTGGCAGTTTCACCAAATAGCTAG
Protein sequence

>Msa0317230

MASKDVWIRKQQCPCGDWKCYITNEGDDSDEGSAAPESVKADKDKTLSDIITPYVGMVFKSDDDAFEHYGNFARKHGFSMRKARSRISPQLGVYKRDFVCYRSGFAPVKRKANGEHHRDRKSVRCGCDAKMYLSKEVVDGVSQWIVLQFSNVHNHELLEDDQVRLLPAYRKIHEADQERILLLSKAGFPVHRIVKMLELEKGIQGGHLSFLERDVRNFVQNRKKVIQENEALLNEKRENDVLELLELCKATKEADDDFVYDFTMDENEKVENIAWSYGDSVNKNVVFGDVIYFDTSYRSITYGFLFGVWFGINSCGRTIFFGCVLLQDETPQSFSWALQVFLRFMKGRCPQTILSDLDPGLKDAIRSEFPETKHVIPVWNILNKVSSWFCLPLGPRYAEFKSTFNALFLIEKTEEFELKWSQMVSDFGLGSDKHIDLLYSVRAYWAPSYVRGYFLARMSIIAFSKSIDAFMKEIVTEHTCLRSFFEQVGISANFHPQSHKETQCTNLRTGIPIEEHARSILTPYAFDALQQELVLTMQYATSELYNGSYIVRHFKKLDGERHVLWLPEKEQILCSCKEFESSGILCRHALRILVTKNYFQLPDKYYLSRWHRERSLNVEDEHNNQSFIAKKWFQEYQSQAENLFSESSITKERSDYARKELMKELTRILNEVRDMSKTDGVETDGVCSMNVAVSPNS*