AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0318850

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0318850 MtrunA17_Chr2g0292731 88.455 563 54 1 148 710 1 552 0.0 1038
Msa0318850 MtrunA17_Chr2g0292741 85.946 555 62 2 156 710 1 539 0.0 991
Msa0318850 MtrunA17_Chr6g0481041 40.000 740 393 16 1 706 1 723 9.49e-171 507
Msa0318850 MtrunA17_Chr2g0322731 36.953 709 412 15 9 706 81 765 3.41e-144 440
Msa0318850 MtrunA17_Chr4g0024351 35.844 717 411 17 6 706 46 729 5.39e-144 438
Msa0318850 MtrunA17_Chr8g0350321 35.901 727 416 21 9 706 101 806 4.58e-136 420
Msa0318850 MtrunA17_Chr7g0246631 35.586 725 413 18 9 706 77 774 6.70e-136 418
Msa0318850 MtrunA17_Chr1g0203671 34.126 715 417 18 25 706 96 789 3.78e-125 391
Msa0318850 MtrunA17_Chr5g0410711 33.244 743 440 21 1 709 61 781 2.10e-124 389
Msa0318850 MtrunA17_Chr2g0331581 32.555 685 418 13 28 706 143 789 1.01e-116 369
Msa0318850 MtrunA17_Chr6g0472471 32.500 760 424 24 2 706 40 765 2.37e-116 367
Msa0318850 MtrunA17_Chr2g0292761 37.013 616 302 17 103 707 1 541 7.31e-115 357
Msa0318850 MtrunA17_Chr5g0394111 32.514 692 366 17 32 707 41 647 1.43e-103 330
Msa0318850 MtrunA17_Chr7g0224451 36.714 493 238 12 25 509 17 443 1.31e-91 292
Msa0318850 MtrunA17_Chr2g0304191 88.971 136 15 0 230 365 1 136 6.14e-81 253
Msa0318850 MtrunA17_Chr7g0224481 36.675 379 176 9 25 395 17 339 1.20e-64 218
Msa0318850 MtrunA17_Chr7g0224491 37.188 320 163 7 398 706 1 293 1.69e-55 191
Msa0318850 MtrunA17_Chr8g0349941 95.556 45 2 0 465 509 67 111 2.85e-23 95.1
Msa0318850 MtrunA17_Chr2g0319671 31.579 228 132 7 226 449 63 270 6.55e-23 100
Msa0318850 MtrunA17_Chr6g0460611 45.055 91 50 0 85 175 2 92 2.07e-22 92.0
Msa0318850 MtrunA17_Chr5g0419821 47.000 100 52 1 23 121 32 131 5.20e-19 85.1
Msa0318850 MtrunA17_Chr2g0319681 26.638 229 160 4 482 706 1 225 2.34e-17 82.4
Msa0318850 MtrunA17_Chr7g0224431 31.818 154 63 4 249 401 4 116 8.15e-13 65.9
Msa0318850 MtrunA17_Chr2g0319691 30.702 114 76 2 336 449 1 111 5.93e-12 64.3
Msa0318850 MtrunA17_Chr2g0319701 25.946 185 117 5 533 706 48 223 6.55e-12 66.2
Msa0318850 MtrunA17_Chr1g0205981 28.837 215 118 6 79 283 1 190 4.68e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0318850 AT1G73360.1 38.787 709 385 14 22 706 30 713 1.77e-160 481
Msa0318850 AT1G17920.2 37.791 688 388 15 28 706 25 681 7.68e-147 444
Msa0318850 AT1G17920.1 37.791 688 388 15 28 706 25 681 7.68e-147 444
Msa0318850 AT1G05230.3 36.506 704 402 13 8 706 51 714 1.81e-146 444
Msa0318850 AT3G03260.1 38.207 725 388 17 7 706 5 694 2.25e-146 443
Msa0318850 AT1G05230.8 36.402 706 402 14 8 706 51 716 1.87e-144 439
Msa0318850 AT1G05230.4 36.402 706 402 14 8 706 51 716 1.87e-144 439
Msa0318850 AT1G05230.9 36.402 706 402 14 8 706 51 716 1.87e-144 439
Msa0318850 AT1G05230.10 36.402 706 402 14 8 706 51 716 1.87e-144 439
Msa0318850 AT1G05230.7 36.402 706 402 14 8 706 51 716 1.87e-144 439
Msa0318850 AT1G05230.11 36.402 706 402 14 8 706 51 716 1.87e-144 439
Msa0318850 AT1G05230.2 36.402 706 402 14 8 706 51 716 1.87e-144 439
Msa0318850 AT1G05230.6 36.402 706 402 14 8 706 51 716 1.87e-144 439
Msa0318850 AT1G05230.5 36.402 706 402 14 8 706 51 716 1.87e-144 439
Msa0318850 AT1G05230.1 36.402 706 402 14 8 706 51 716 1.87e-144 439
Msa0318850 AT4G04890.2 36.401 728 414 17 2 706 34 735 2.27e-142 434
Msa0318850 AT4G04890.1 36.401 728 414 17 2 706 34 735 2.27e-142 434
Msa0318850 AT4G21750.2 36.555 714 402 14 25 706 63 757 4.32e-139 427
Msa0318850 AT4G21750.1 36.555 714 402 14 25 706 63 757 4.32e-139 427
Msa0318850 AT4G21750.3 36.555 714 402 14 25 706 63 757 4.32e-139 427
Msa0318850 AT4G21750.4 36.555 714 402 14 25 706 63 757 4.32e-139 427
Msa0318850 AT5G17320.1 34.459 740 426 20 1 706 1 715 1.95e-126 392
Msa0318850 AT4G00730.1 34.282 703 416 18 20 706 126 798 5.27e-125 391
Msa0318850 AT3G61150.1 33.379 731 428 21 11 706 97 803 1.36e-123 387
Msa0318850 AT1G05230.12 36.192 583 336 10 8 586 51 601 1.11e-120 374
Msa0318850 AT1G34650.1 35.302 711 395 20 32 706 24 705 2.84e-118 371
Msa0318850 AT3G03260.2 39.714 559 303 12 7 556 5 538 3.00e-117 363
Msa0318850 AT5G52170.3 34.225 710 380 20 11 706 42 678 2.07e-115 362
Msa0318850 AT5G52170.1 34.225 710 380 20 11 706 42 678 2.07e-115 362
Msa0318850 AT5G52170.2 34.266 715 378 21 11 706 42 683 1.26e-114 360
Msa0318850 AT5G46880.3 31.474 753 431 21 8 710 93 810 1.29e-109 351
Msa0318850 AT5G46880.1 31.474 753 431 21 8 710 93 810 1.29e-109 351
Msa0318850 AT5G46880.2 31.474 753 431 21 8 710 93 810 1.29e-109 351
Msa0318850 AT2G32370.1 32.055 730 415 22 8 704 37 718 1.27e-104 335
Msa0318850 AT1G79840.3 32.565 694 403 19 25 706 44 684 2.44e-104 334
Msa0318850 AT1G79840.6 32.565 694 403 19 25 706 44 684 2.44e-104 334
Msa0318850 AT1G79840.1 32.565 694 403 19 25 706 102 742 9.01e-104 334
Msa0318850 AT1G79840.2 32.565 694 403 19 25 706 131 771 2.82e-103 333
Msa0318850 AT4G17710.1 30.496 705 389 20 23 710 87 707 1.29e-95 311
Msa0318850 AT4G25530.1 29.196 709 449 16 8 706 17 682 7.11e-88 290
Msa0318850 AT1G79840.4 32.570 568 326 14 25 584 102 620 1.50e-87 288
Msa0318850 AT4G00730.2 35.581 430 248 9 25 447 135 542 1.79e-80 267
Msa0318850 AT1G79840.5 32.143 448 249 12 25 464 102 502 5.13e-60 211
Msa0318850 AT5G07260.1 20.755 636 391 17 83 706 3 537 4.20e-25 110
Msa0318850 AT5G07260.2 20.411 632 393 15 83 706 3 532 4.87e-25 110

Find 164 sgRNAs with CRISPR-Local

Find 284 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCACCAATCCTGAATATT+TGG 0.136860 2_4:-17992559 None:intergenic
CCAAAGAACAATCACTTTAA+AGG 0.153190 2_4:+17992513 Msa0318850:CDS
ATGGACTGGTGCTCAGAATT+TGG 0.173529 2_4:-17994781 None:intergenic
AAATTATGGATGTGGCTTTA+AGG 0.186919 2_4:+17994627 Msa0318850:CDS
ATCGCTTTGTAGGATCTTTA+AGG 0.190512 2_4:-17994082 None:intergenic
TAGTGGGATGGTCTTTCTTA+TGG 0.230215 2_4:+17994299 Msa0318850:intron
GTGTACCCATCTTGAGTATT+TGG 0.232783 2_4:-17992459 None:intergenic
AAACCAAAACTTGATTTGTT+TGG 0.252374 2_4:-17991246 None:intergenic
TTTGGAGATTTGACCCAAAA+AGG 0.264891 2_4:-17992441 None:intergenic
CCTTTAAAGTGATTGTTCTT+TGG 0.271566 2_4:-17992513 None:intergenic
AACCAAAACTTGATTTGTTT+GGG 0.288547 2_4:-17991245 None:intergenic
GAAGAATCTTCCAAATATTC+AGG 0.291428 2_4:+17992549 Msa0318850:CDS
GTTACCTGTGATCGCTTTGT+AGG 0.304101 2_4:-17994092 None:intergenic
AGCCCAAACAAATCAAGTTT+TGG 0.306856 2_4:+17991243 Msa0318850:CDS
TATTCCTCCCTTCAAGTGTT+TGG 0.321521 2_4:-17993839 None:intergenic
GTTGGCGACTATAATTTATA+CGG 0.324947 2_4:+17993588 Msa0318850:CDS
TGACGTCGTTCTGCCTCGTC+TGG 0.329592 2_4:-17991197 None:intergenic
AAAGCTCCATCTCGACTTCC+TGG 0.342734 2_4:-17992921 None:intergenic
AATTATGGATGTGGCTTTAA+GGG 0.342930 2_4:+17994628 Msa0318850:CDS
CGGAGCTGAAAGCTGGATTA+AGG 0.349348 2_4:+17993608 Msa0318850:CDS
CCTCCTATGGTTTCTTCAAC+AGG 0.351635 2_4:-17993681 None:intergenic
TGAAATGCAGTTGCCATGAT+AGG 0.356217 2_4:-17994360 None:intergenic
CAATGCTATCAAGAGTTAAA+TGG 0.356804 2_4:-17993938 None:intergenic
TCAGCTTTGGTAACAATTGT+AGG 0.366355 2_4:-17992870 None:intergenic
TGGACTGGTGCTCAGAATTT+GGG 0.370714 2_4:-17994780 None:intergenic
TTACTGCTGCTACCACCATT+TGG 0.373536 2_4:+17994027 Msa0318850:CDS
CCAAATCTTAATGCACCATT+TGG 0.373891 2_4:+17994713 Msa0318850:CDS
TCGAGATGGAGCTTTATTAC+TGG 0.373995 2_4:+17992929 Msa0318850:CDS
CACTTTGATAGTTTCAGCTT+TGG 0.378308 2_4:-17992883 None:intergenic
TTCATGGTAATCTAACTATA+AGG 0.391331 2_4:+17992304 Msa0318850:CDS
CTCTATACATGTAATGTGTA+TGG 0.392878 2_4:-17993560 None:intergenic
ATTAGTAACTTTCGAGATGA+TGG 0.395166 2_4:+17990088 Msa0318850:CDS
ACTATCAAAGTGTTTGAAAT+CGG 0.396566 2_4:+17992894 Msa0318850:CDS
CCAAATGGTGCATTAAGATT+TGG 0.397130 2_4:-17994713 None:intergenic
AGAATGTGTGAGAGATCTTT+AGG 0.399513 2_4:+17993639 Msa0318850:CDS
GTCAATTGGCTGTGAAAGTT+GGG 0.402996 2_4:+17991216 Msa0318850:CDS
GAGTGCTCAAATCATGTTCA+TGG 0.409107 2_4:+17992288 Msa0318850:CDS
TTAGAATAGTAGCTGCCAAT+TGG 0.411318 2_4:-17994853 None:intergenic
CACTCATAAACCGTTCGGTT+TGG 0.412582 2_4:-17992270 None:intergenic
AGCACTTGGTCAACTGCATA+TGG 0.416823 2_4:-17994800 None:intergenic
CCACAATTTGCGAAGCTTGT+GGG 0.416831 2_4:+17991941 Msa0318850:CDS
CGTCAATTGGCTGTGAAAGT+TGG 0.419536 2_4:+17991215 Msa0318850:CDS
GAAATTGCACACATTTCAAA+TGG 0.421250 2_4:+17994335 Msa0318850:CDS
AGCGGAGGTAAGACGTTTGT+AGG 0.421913 2_4:-17990122 None:intergenic
GAACAAATAACAAAACCATT+TGG 0.423668 2_4:-17994680 None:intergenic
CGAGGCAGAACGACGTCAAT+TGG 0.430389 2_4:+17991202 Msa0318850:CDS
CGAGAAAGAGGTGCAGTGTT+TGG 0.431308 2_4:-17993175 None:intergenic
AAATCTCCAAATACTCAAGA+TGG 0.432047 2_4:+17992453 Msa0318850:CDS
GAAGATTAGTGGCATAGATT+TGG 0.434141 2_4:+17992578 Msa0318850:CDS
ACAAAACCATTTGGAAGGAT+TGG 0.438392 2_4:-17994671 None:intergenic
TCAGGATTGGTGAAGATTAG+TGG 0.444549 2_4:+17992567 Msa0318850:CDS
TAGAATAGTAGCTGCCAATT+GGG 0.446699 2_4:-17994852 None:intergenic
ATGGAAGGTGGCGGTGAGTC+CGG 0.447690 2_4:+17990055 Msa0318850:CDS
ATTTATACGGAGCTGAAAGC+TGG 0.450930 2_4:+17993601 Msa0318850:CDS
AGATGCTGCAATGGATGAAT+TGG 0.453548 2_4:+17992395 Msa0318850:CDS
TCTCCTGCACGAAATGTTGA+TGG 0.453739 2_4:-17991987 None:intergenic
TACTTATATCAACACCCAAT+TGG 0.455805 2_4:+17994838 Msa0318850:CDS
GCAGTAACAACAGTGCCATT+TGG 0.456929 2_4:-17994011 None:intergenic
CACAATTTGCGAAGCTTGTG+GGG 0.460810 2_4:+17991942 Msa0318850:CDS
AAAGTGTGATTAACCTCCTA+TGG 0.462791 2_4:-17993694 None:intergenic
GTGTTTGAAATCGGTTCGCC+AGG 0.467602 2_4:+17992903 Msa0318850:CDS
CAACTGCATTTCAATTATCA+AGG 0.467731 2_4:+17994370 Msa0318850:CDS
CATCTTCCACATTAAGCACT+TGG 0.467910 2_4:-17994814 None:intergenic
TCATAAACCGTTCGGTTTGG+TGG 0.470954 2_4:-17992267 None:intergenic
GAACAAGAGAACTCTGTTGA+AGG 0.473464 2_4:+17991271 Msa0318850:CDS
CATTTAACTCTTGATAGCAT+TGG 0.474351 2_4:+17993939 Msa0318850:CDS
GAAAAGTTGATGCTGGTGTA+TGG 0.477684 2_4:+17993119 Msa0318850:CDS
CTGCACCTCTTTCTCGTGGT+TGG 0.478289 2_4:+17993182 Msa0318850:CDS
TTTGGCTCCCTCTGCCTGCT+CGG 0.481429 2_4:+17994045 Msa0318850:CDS
TAACAACAGTGCCATTTGGC+TGG 0.481596 2_4:-17994007 None:intergenic
TCAGTGATCCAAACACTTGA+AGG 0.482426 2_4:+17993831 Msa0318850:intron
TTAAGAGCTTGCTCAAACTC+CGG 0.486029 2_4:-17992204 None:intergenic
TTGAGCACTCATAAACCGTT+CGG 0.491484 2_4:-17992275 None:intergenic
TGTGAATGCTTAACAATGCC+TGG 0.491755 2_4:+17993900 Msa0318850:CDS
TGATGGTCTTCCACAGGAAA+TGG 0.495341 2_4:-17991970 None:intergenic
CCCACAAGCTTCGCAAATTG+TGG 0.495588 2_4:-17991941 None:intergenic
ATGTGTGCAATTTCATGCAT+TGG 0.495739 2_4:-17994326 None:intergenic
TCATTCGTGAAATGCCTCAT+GGG 0.496695 2_4:+17993224 Msa0318850:CDS
GAGTTACCAATCCTTCCAAA+TGG 0.500630 2_4:+17994665 Msa0318850:CDS
TACATGTATAGAGATCTTGT+TGG 0.502405 2_4:+17993570 Msa0318850:CDS
CAGCTTTGGTAACAATTGTA+GGG 0.502451 2_4:-17992869 None:intergenic
GGTTGGCATGTTTCTCGACT+CGG 0.505106 2_4:+17992599 Msa0318850:CDS
TATATAATGTATGATCCTAC+AGG 0.508050 2_4:-17994587 None:intergenic
ACTATAAGGAGAGGTGTGCT+AGG 0.512568 2_4:+17992318 Msa0318850:CDS
CGTGGTTGGAAACATCCCTC+TGG 0.513481 2_4:+17993196 Msa0318850:CDS
GTCGTTCTGCCTCGTCTGGA+TGG 0.514221 2_4:-17991193 None:intergenic
CGAAAATCTCAAAATAAAAG+AGG 0.514839 2_4:+17991909 Msa0318850:CDS
ATTTCAAATGGACCCTATCA+TGG 0.514869 2_4:+17994347 Msa0318850:CDS
TTAACTCTTGATAGCATTGG+TGG 0.517292 2_4:+17993942 Msa0318850:CDS
TTGAGAAATGGATTACTCAA+TGG 0.517728 2_4:+17990036 None:intergenic
CTGCAATGGATGAATTGGTT+AGG 0.518596 2_4:+17992400 Msa0318850:CDS
AAAACTTTCCGAGCAGGCAG+AGG 0.518954 2_4:-17994053 None:intergenic
GATGATACAAGCCAGCCAAA+TGG 0.520622 2_4:+17993996 Msa0318850:CDS
CTTAACAATGCCTGGTCAAG+TGG 0.522028 2_4:+17993908 Msa0318850:CDS
ATCTTCCAAATATTCAGGAT+TGG 0.522094 2_4:+17992554 Msa0318850:CDS
AGGTGCAGTGTTTGGTCGAG+AGG 0.523583 2_4:-17993167 None:intergenic
CATCCCTACTCAGAGGCAAA+TGG 0.525201 2_4:+17994538 Msa0318850:CDS
TCACCATTTGCCTCTGAGTA+GGG 0.526700 2_4:-17994541 None:intergenic
AACTCTTGATAGCATTGGTG+GGG 0.529682 2_4:+17993944 Msa0318850:CDS
GCCACAATTTGCGAAGCTTG+TGG 0.529984 2_4:+17991940 Msa0318850:CDS
GAGGTAAGACGTTTGTAGGA+AGG 0.532797 2_4:-17990118 None:intergenic
ATTAGTGGCATAGATTTGGT+TGG 0.532831 2_4:+17992582 Msa0318850:CDS
AAATAACAAAACCATTTGGA+AGG 0.532923 2_4:-17994676 None:intergenic
TGGCTGGCTTGTATCATCGT+CGG 0.539237 2_4:-17993991 None:intergenic
TAACTCTTGATAGCATTGGT+GGG 0.540658 2_4:+17993943 Msa0318850:CDS
GAAATGCAGTTGCCATGATA+GGG 0.544552 2_4:-17994359 None:intergenic
ATCATTCGTGAAATGCCTCA+TGG 0.548977 2_4:+17993223 Msa0318850:CDS
TTTCTGATGAAGTTTCATGA+AGG 0.549807 2_4:+17991163 Msa0318850:intron
GGTCACTTGGGTTGAACATG+TGG 0.552782 2_4:+17993521 Msa0318850:intron
ATACTGTCAGATGCTCCAAA+TGG 0.553262 2_4:-17994728 None:intergenic
GAAAGTTTCACTTCTCCTGT+AGG 0.556978 2_4:+17994572 Msa0318850:CDS
TACTGCAGAAAAGTTGATGC+TGG 0.557695 2_4:+17993112 Msa0318850:CDS
ATTATGGATGTGGCTTTAAG+GGG 0.559199 2_4:+17994629 Msa0318850:CDS
GATAAAGCTTGCTGATAGAA+TGG 0.559844 2_4:+17993866 Msa0318850:CDS
GTTACTAATGTCTTCATCAC+CGG 0.562506 2_4:-17990074 None:intergenic
AGCAGGCAGAGGGAGCCAAA+TGG 0.567621 2_4:-17994042 None:intergenic
TTCAAGTATGGATGTTTGAG+CGG 0.568957 2_4:-17990140 None:intergenic
ATGTGAAGGATGCTCTTATG+AGG 0.569555 2_4:+17994876 Msa0318850:CDS
TTGGGTTGAACATGTGGAAG+TGG 0.571125 2_4:+17993527 Msa0318850:CDS
TGGTTAAACTCCACTTGACC+AGG 0.571210 2_4:-17993918 None:intergenic
AGATCCTACAAAGCGATCAC+AGG 0.573425 2_4:+17994088 Msa0318850:CDS
TGTTTCCAACCACGAGAAAG+AGG 0.576681 2_4:-17993187 None:intergenic
AGCTACTATTCTAAATGTGA+AGG 0.578993 2_4:+17994862 Msa0318850:CDS
AGACCATCAACATTTCGTGC+AGG 0.579781 2_4:+17991984 Msa0318850:CDS
AATCTCCAAATACTCAAGAT+GGG 0.583747 2_4:+17992454 Msa0318850:CDS
AAAAGTTGATGCTGGTGTAT+GGG 0.585328 2_4:+17993120 Msa0318850:CDS
AAATGTTGATGGTCTTCCAC+AGG 0.587353 2_4:-17991976 None:intergenic
AATTCGCGAGGCCTCACTAG+AGG 0.588079 2_4:-17993076 None:intergenic
GCATCTGACAGTATTGAAGA+TGG 0.589389 2_4:+17994737 Msa0318850:CDS
TTGGTCAACTGCATATGGAC+TGG 0.592188 2_4:-17994795 None:intergenic
CTATAAGGAGAGGTGTGCTA+GGG 0.592409 2_4:+17992319 Msa0318850:CDS
TCTGCATGTAACTTGCAAGA+AGG 0.593980 2_4:-17992167 None:intergenic
TGAAATTCCACCAAACCGAA+CGG 0.595042 2_4:+17992260 Msa0318850:CDS
AAACTTTCCGAGCAGGCAGA+GGG 0.595721 2_4:-17994052 None:intergenic
AAATGCTCAGCTGAAACAAG+AGG 0.596460 2_4:+17992020 Msa0318850:CDS
ATGGTGCATTAAGATTTGGT+TGG 0.601403 2_4:-17994709 None:intergenic
ATCACCATTTGCCTCTGAGT+AGG 0.601759 2_4:-17994542 None:intergenic
AGATGGCTCGATGTTAACAT+TGG 0.603127 2_4:+17994754 Msa0318850:CDS
AACACTGCACCTCTTTCTCG+TGG 0.603163 2_4:+17993178 Msa0318850:CDS
CAGTGATCCAAACACTTGAA+GGG 0.609013 2_4:+17993832 Msa0318850:intron
CATTCGTGAAATGCCTCATG+GGG 0.609503 2_4:+17993225 Msa0318850:CDS
GGTTCGCCAGGAAGTCGAGA+TGG 0.615372 2_4:+17992915 Msa0318850:CDS
AAGTATGGATGTTTGAGCGG+AGG 0.615778 2_4:-17990137 None:intergenic
GAAATGGATTACTCAATGGA+AGG 0.616989 2_4:+17990040 None:intergenic
TGGTCTTCCACAGGAAATGG+TGG 0.617088 2_4:-17991967 None:intergenic
TCAAGTTGTAGATGCTGCAA+TGG 0.619732 2_4:+17992386 Msa0318850:CDS
GCAGAAAGAGATAACACTAC+CGG 0.621791 2_4:+17992185 Msa0318850:CDS
GTTCTGCCTCGTCTGGATGG+TGG 0.624308 2_4:-17991190 None:intergenic
GATTACTCAATGGAAGGTGG+CGG 0.624947 2_4:+17990046 Msa0318850:CDS
ATACCTGTTGAAGAAACCAT+AGG 0.625785 2_4:+17993678 Msa0318850:CDS
AGGCAGAGGGAGCCAAATGG+TGG 0.636325 2_4:-17994039 None:intergenic
CCTGTTGAAGAAACCATAGG+AGG 0.639101 2_4:+17993681 Msa0318850:CDS
TCACGAATGATGCATCCAGA+GGG 0.641603 2_4:-17993211 None:intergenic
CTGGCTTGTATCATCGTCGG+TGG 0.642130 2_4:-17993988 None:intergenic
ACAATTTGCGAAGCTTGTGG+GGG 0.652499 2_4:+17991943 Msa0318850:CDS
TTCACGAATGATGCATCCAG+AGG 0.657086 2_4:-17993212 None:intergenic
GGTAATCTAACTATAAGGAG+AGG 0.660462 2_4:+17992309 Msa0318850:CDS
ATGGATTACTCAATGGAAGG+TGG 0.666414 2_4:+17990043 Msa0318850:CDS
GGAATGCCACCATCCAGACG+AGG 0.674051 2_4:+17991184 Msa0318850:CDS
CGAATGATGTTAAATTCGCG+AGG 0.685235 2_4:-17993088 None:intergenic
TGATCCAAACACTTGAAGGG+AGG 0.696996 2_4:+17993835 Msa0318850:CDS
AGTCATTCATCCCTACTCAG+AGG 0.722493 2_4:+17994531 Msa0318850:intron
ATTCGTGAAATGCCTCATGG+GGG 0.745536 2_4:+17993226 Msa0318850:CDS
AGTTGACCAAGTGCTTAATG+TGG 0.750936 2_4:+17994808 Msa0318850:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAATATATAAAAAAAAA+AGG - chr2_4:17990669-17990688 None:intergenic 0.0%
!!! AGTTTTTTTTTTTTTTTTTG+TGG + chr2_4:17990526-17990545 Msa0318850:intron 10.0%
!! AAAAAGAAATATTAAAAGTT+CGG - chr2_4:17991473-17991492 None:intergenic 10.0%
!!! TTTTCTATTAGTAATTTTAT+GGG - chr2_4:17991510-17991529 None:intergenic 10.0%
!!! TGTTTTTATCTATAATTTTT+CGG + chr2_4:17992722-17992741 Msa0318850:intron 10.0%
!!! AAAAAAAGCATTTAAAATTT+GGG - chr2_4:17993021-17993040 None:intergenic 10.0%
!!! TAATATAATTTAGAGTATAA+GGG - chr2_4:17993417-17993436 None:intergenic 10.0%
!!! AGTATAAATTTTAAAAGAAA+TGG - chr2_4:17994402-17994421 None:intergenic 10.0%
!! TAATTAATTATATAAGTGAT+TGG - chr2_4:17994494-17994513 None:intergenic 10.0%
!!! AATTAATTATTTTTTTCATG+TGG + chr2_4:17994505-17994524 Msa0318850:intron 10.0%
!!! TATTCAATTTTTGATTTTGA+AGG + chr2_4:17990220-17990239 Msa0318850:intron 15.0%
!!! TATTCAATTTTTGATTTTGA+AGG + chr2_4:17990220-17990239 Msa0318850:intron 15.0%
!!! TTTTTTTTTTTTTTTTGTGG+TGG + chr2_4:17990529-17990548 Msa0318850:intron 15.0%
!! TATTTCAAACCTATATAAAA+AGG - chr2_4:17990699-17990718 None:intergenic 15.0%
!! TACTAACTATATTTAATGTA+TGG + chr2_4:17990837-17990856 Msa0318850:intron 15.0%
!!! TTCGAATAATTAATTTTGTA+TGG + chr2_4:17990868-17990887 Msa0318850:intron 15.0%
!!! GTTTTCTATTAGTAATTTTA+TGG - chr2_4:17991511-17991530 None:intergenic 15.0%
!!! ATTGTGTTTTTTTTTTTTTC+AGG + chr2_4:17991540-17991559 Msa0318850:intron 15.0%
!! CCAAATTTACAAATATAATT+AGG - chr2_4:17992117-17992136 None:intergenic 15.0%
!! CCTAATTATATTTGTAAATT+TGG + chr2_4:17992114-17992133 Msa0318850:intron 15.0%
!!! GATATATAACTCATTTTTAT+AGG + chr2_4:17992827-17992846 Msa0318850:intron 15.0%
!!! CAAAAAAAGCATTTAAAATT+TGG - chr2_4:17993022-17993041 None:intergenic 15.0%
!!! CAAATTTTAAATGCTTTTTT+TGG + chr2_4:17993020-17993039 Msa0318850:intron 15.0%
!!! CTAATATAATTTAGAGTATA+AGG - chr2_4:17993418-17993437 None:intergenic 15.0%
!!! CATATATACTATACATTTTT+AGG + chr2_4:17993500-17993519 Msa0318850:intron 15.0%
!! AGCTAAATATAAAATTTACA+AGG - chr2_4:17993764-17993783 None:intergenic 15.0%
!!! TGTTAATAATTTTGTTATAG+TGG + chr2_4:17994282-17994301 Msa0318850:intron 15.0%
!!! GTTAATAATTTTGTTATAGT+GGG + chr2_4:17994283-17994302 Msa0318850:intron 15.0%
!!! AAGATTACTCATTTTGTTAA+CGG + chr2_4:17990167-17990186 Msa0318850:intron 20.0%
!!! CTTTTGAGTTTAATGAAAAA+CGG + chr2_4:17990334-17990353 Msa0318850:intron 20.0%
!!! TTCACAAAACCTTTTTATAT+AGG + chr2_4:17990687-17990706 Msa0318850:intron 20.0%
!!! TGTTTTAATTTTCCTTTGTT+TGG + chr2_4:17990782-17990801 Msa0318850:intron 20.0%
!!! GTTGAATTTAAAATCATGAA+TGG + chr2_4:17990890-17990909 Msa0318850:intron 20.0%
!! ACGATGAAATTAAACTAAAA+TGG - chr2_4:17990925-17990944 None:intergenic 20.0%
!! CTTAAACACTTATGAATATA+TGG + chr2_4:17990974-17990993 Msa0318850:intron 20.0%
!!! ATTTTGACTATAAAGTGAAA+AGG - chr2_4:17991306-17991325 None:intergenic 20.0%
!! AACTTATTCAAATATCCAAA+TGG - chr2_4:17991341-17991360 None:intergenic 20.0%
!! ATAATTAGGTATTTATGCAT+GGG - chr2_4:17992103-17992122 None:intergenic 20.0%
!! TATAATTAGGTATTTATGCA+TGG - chr2_4:17992104-17992123 None:intergenic 20.0%
!! TCACAAAATATTAATGAAAG+AGG - chr2_4:17992781-17992800 None:intergenic 20.0%
!!! TGGGAAAAAATTTTATAATC+AGG - chr2_4:17993002-17993021 None:intergenic 20.0%
!!! TATATTTCTAAGAGAATTGA+TGG + chr2_4:17993270-17993289 Msa0318850:intron 20.0%
!!! TTTTTCTTGGTTGTATATAT+GGG + chr2_4:17993383-17993402 Msa0318850:intron 20.0%
!! AATAGATGAATAGAGATATT+TGG - chr2_4:17993739-17993758 None:intergenic 20.0%
!! AGTTCACATATAATTGTTAA+TGG + chr2_4:17994254-17994273 Msa0318850:intron 20.0%
!!! GTAATCTTACTTTTCAAGTA+TGG - chr2_4:17990155-17990174 None:intergenic 25.0%
!!! ACTTGATTGTTTGATTTTGA+AGG - chr2_4:17990491-17990510 None:intergenic 25.0%
!!! TTTTTTTTTTTTGTGGTGGT+CGG + chr2_4:17990533-17990552 Msa0318850:intron 25.0%
! GATAACGCATAATATATACA+AGG - chr2_4:17990574-17990593 None:intergenic 25.0%
! ATATATGGAAGCTAATTAGA+TGG + chr2_4:17990989-17991008 Msa0318850:intron 25.0%
! AGATGGATATGTATTTAAAG+TGG + chr2_4:17991006-17991025 Msa0318850:intron 25.0%
!! GATATAGCCTTTTTAATAAG+TGG - chr2_4:17991054-17991073 None:intergenic 25.0%
!!! AACCAAAACTTGATTTGTTT+GGG - chr2_4:17991248-17991267 None:intergenic 25.0%
!!! AAACCAAAACTTGATTTGTT+TGG - chr2_4:17991249-17991268 None:intergenic 25.0%
! AATTTGAGTAAACATCCATT+TGG + chr2_4:17991323-17991342 Msa0318850:intron 25.0%
! GGATATTTGAATAAGTTAGA+AGG + chr2_4:17991344-17991363 Msa0318850:intron 25.0%
! TATTTGAATAAGTTAGAAGG+TGG + chr2_4:17991347-17991366 Msa0318850:intron 25.0%
! ATTACACATGCTTTCATATA+TGG + chr2_4:17991622-17991641 Msa0318850:intron 25.0%
!!! GTGTAGTTTTTGAAAAATCA+CGG - chr2_4:17991689-17991708 None:intergenic 25.0%
! AAAAACTACACTATCTATCA+TGG + chr2_4:17991698-17991717 Msa0318850:intron 25.0%
! TATTAGCACTAAGCATATAT+TGG - chr2_4:17991758-17991777 None:intergenic 25.0%
! ACATAAAATTATTAGCACCA+AGG - chr2_4:17991815-17991834 None:intergenic 25.0%
! CGAAAATCTCAAAATAAAAG+AGG + chr2_4:17991909-17991928 Msa0318850:CDS 25.0%
! TTCATGGTAATCTAACTATA+AGG + chr2_4:17992304-17992323 Msa0318850:CDS 25.0%
!!! ACTCATTTTTATAGGTCAAA+TGG + chr2_4:17992835-17992854 Msa0318850:intron 25.0%
!! ACTATCAAAGTGTTTGAAAT+CGG + chr2_4:17992894-17992913 Msa0318850:CDS 25.0%
!!! TTTCCTATTTCTCTTTTTCT+TGG + chr2_4:17993370-17993389 Msa0318850:intron 25.0%
!! CTTTTTCTTGGTTGTATATA+TGG + chr2_4:17993382-17993401 Msa0318850:intron 25.0%
!!! TATACATTTTTAGGTCACTT+GGG + chr2_4:17993509-17993528 Msa0318850:intron 25.0%
! ATGAATAGAGATATTTGGAA+GGG - chr2_4:17993734-17993753 None:intergenic 25.0%
!! TTATAATTTAGAGCAAACTG+TGG + chr2_4:17994125-17994144 Msa0318850:intron 25.0%
!!! ATAATTTTGTTATAGTGGGA+TGG + chr2_4:17994287-17994306 Msa0318850:intron 25.0%
! TATATACCGAACAATTTCTA+GGG - chr2_4:17994460-17994479 None:intergenic 25.0%
! TATATAATGTATGATCCTAC+AGG - chr2_4:17994590-17994609 None:intergenic 25.0%
! TCCAACTGATAGAAAAATTA+TGG + chr2_4:17994613-17994632 Msa0318850:CDS 25.0%
!! TCCATAATTTTTCTATCAGT+TGG - chr2_4:17994617-17994636 None:intergenic 25.0%
! TGATAGAAAAATTATGGATG+TGG + chr2_4:17994619-17994638 Msa0318850:CDS 25.0%
! AAATAACAAAACCATTTGGA+AGG - chr2_4:17994679-17994698 None:intergenic 25.0%
! GAACAAATAACAAAACCATT+TGG - chr2_4:17994683-17994702 None:intergenic 25.0%
ATTAGTAACTTTCGAGATGA+TGG + chr2_4:17990088-17990107 Msa0318850:CDS 30.0%
!!! AGTTTTGAGAATTCAAGAGA+AGG - chr2_4:17990375-17990394 None:intergenic 30.0%
!!! TTTTTTTTTTTGTGGTGGTC+GGG + chr2_4:17990534-17990553 Msa0318850:intron 30.0%
CATAATATATACAAGGTCCA+GGG - chr2_4:17990567-17990586 None:intergenic 30.0%
AAAGGAATTAAGCCAAACAA+AGG - chr2_4:17990797-17990816 None:intergenic 30.0%
! TTTGTTTGGCTTAATTCCTT+TGG + chr2_4:17990796-17990815 Msa0318850:intron 30.0%
GACTGAACCACTTATTAAAA+AGG + chr2_4:17991044-17991063 Msa0318850:intron 30.0%
TTTCTGATGAAGTTTCATGA+AGG + chr2_4:17991163-17991182 Msa0318850:intron 30.0%
AAGCATATATTGGTATCATG+TGG - chr2_4:17991748-17991767 None:intergenic 30.0%
AAATCTCCAAATACTCAAGA+TGG + chr2_4:17992453-17992472 Msa0318850:CDS 30.0%
AATCTCCAAATACTCAAGAT+GGG + chr2_4:17992454-17992473 Msa0318850:CDS 30.0%
!! CCTTTAAAGTGATTGTTCTT+TGG - chr2_4:17992516-17992535 None:intergenic 30.0%
CCAAAGAACAATCACTTTAA+AGG + chr2_4:17992513-17992532 Msa0318850:CDS 30.0%
GAAGAATCTTCCAAATATTC+AGG + chr2_4:17992549-17992568 Msa0318850:CDS 30.0%
ATCTTCCAAATATTCAGGAT+TGG + chr2_4:17992554-17992573 Msa0318850:CDS 30.0%
! GAAAAGTGCGCATATAATTT+AGG - chr2_4:17993354-17993373 None:intergenic 30.0%
CAACCAAGAAAAAGAGAAAT+AGG - chr2_4:17993376-17993395 None:intergenic 30.0%
!!! CTATACATTTTTAGGTCACT+TGG + chr2_4:17993508-17993527 Msa0318850:intron 30.0%
CTCTATACATGTAATGTGTA+TGG - chr2_4:17993563-17993582 None:intergenic 30.0%
TACATGTATAGAGATCTTGT+TGG + chr2_4:17993570-17993589 Msa0318850:CDS 30.0%
GTTGGCGACTATAATTTATA+CGG + chr2_4:17993588-17993607 Msa0318850:CDS 30.0%
GATGAATAGAGATATTTGGA+AGG - chr2_4:17993735-17993754 None:intergenic 30.0%
CAATGCTATCAAGAGTTAAA+TGG - chr2_4:17993941-17993960 None:intergenic 30.0%
! CATTTAACTCTTGATAGCAT+TGG + chr2_4:17993939-17993958 Msa0318850:CDS 30.0%
! TTGGTCCTCAAATATGTTTT+TGG + chr2_4:17994212-17994231 Msa0318850:intron 30.0%
GCTAACCAAAAACATATTTG+AGG - chr2_4:17994220-17994239 None:intergenic 30.0%
GAAATTGCACACATTTCAAA+TGG + chr2_4:17994335-17994354 Msa0318850:CDS 30.0%
CAACTGCATTTCAATTATCA+AGG + chr2_4:17994370-17994389 Msa0318850:CDS 30.0%
CTATATACCGAACAATTTCT+AGG - chr2_4:17994461-17994480 None:intergenic 30.0%
! AAATTATGGATGTGGCTTTA+AGG + chr2_4:17994627-17994646 Msa0318850:CDS 30.0%
!! AATTATGGATGTGGCTTTAA+GGG + chr2_4:17994628-17994647 Msa0318850:CDS 30.0%
TACTTATATCAACACCCAAT+TGG + chr2_4:17994838-17994857 Msa0318850:CDS 30.0%
AGCTACTATTCTAAATGTGA+AGG + chr2_4:17994862-17994881 Msa0318850:CDS 30.0%
GTTACTAATGTCTTCATCAC+CGG - chr2_4:17990077-17990096 None:intergenic 35.0%
TTCAAGTATGGATGTTTGAG+CGG - chr2_4:17990143-17990162 None:intergenic 35.0%
!!! TTTTTTTTTTGTGGTGGTCG+GGG + chr2_4:17990535-17990554 Msa0318850:intron 35.0%
GCATAATATATACAAGGTCC+AGG - chr2_4:17990568-17990587 None:intergenic 35.0%
TTATATAGTAGCGTCACCAA+AGG - chr2_4:17990815-17990834 None:intergenic 35.0%
! AGCCCAAACAAATCAAGTTT+TGG + chr2_4:17991243-17991262 Msa0318850:CDS 35.0%
TATCACGTATGCTAGAATCA+TGG + chr2_4:17991656-17991675 Msa0318850:intron 35.0%
AACTACACTATCTATCATGG+TGG + chr2_4:17991701-17991720 Msa0318850:intron 35.0%
CCAAGGAAATTCGATTCTTT+AGG - chr2_4:17991798-17991817 None:intergenic 35.0%
CCTAAAGAATCGAATTTCCT+TGG + chr2_4:17991795-17991814 Msa0318850:intron 35.0%
CAACATGAACGAGAGAAAAA+CGG + chr2_4:17991853-17991872 Msa0318850:CDS 35.0%
GGTAATCTAACTATAAGGAG+AGG + chr2_4:17992309-17992328 Msa0318850:CDS 35.0%
TTTGGAGATTTGACCCAAAA+AGG - chr2_4:17992444-17992463 None:intergenic 35.0%
CTTCACCAATCCTGAATATT+TGG - chr2_4:17992562-17992581 None:intergenic 35.0%
GAAGATTAGTGGCATAGATT+TGG + chr2_4:17992578-17992597 Msa0318850:CDS 35.0%
! ATTAGTGGCATAGATTTGGT+TGG + chr2_4:17992582-17992601 Msa0318850:CDS 35.0%
! CAGCTTTGGTAACAATTGTA+GGG - chr2_4:17992872-17992891 None:intergenic 35.0%
! TCAGCTTTGGTAACAATTGT+AGG - chr2_4:17992873-17992892 None:intergenic 35.0%
! CACTTTGATAGTTTCAGCTT+TGG - chr2_4:17992886-17992905 None:intergenic 35.0%
! ATATTTTATCGCCTCTAGTG+AGG + chr2_4:17993065-17993084 Msa0318850:CDS 35.0%
!! AAAAGTTGATGCTGGTGTAT+GGG + chr2_4:17993120-17993139 Msa0318850:CDS 35.0%
GTGCGCATATAATTTAGGAA+AGG - chr2_4:17993349-17993368 None:intergenic 35.0%
AGAATGTGTGAGAGATCTTT+AGG + chr2_4:17993639-17993658 Msa0318850:CDS 35.0%
ATACCTGTTGAAGAAACCAT+AGG + chr2_4:17993678-17993697 Msa0318850:CDS 35.0%
AAAGTGTGATTAACCTCCTA+TGG - chr2_4:17993697-17993716 None:intergenic 35.0%
!! GATAAAGCTTGCTGATAGAA+TGG + chr2_4:17993866-17993885 Msa0318850:CDS 35.0%
! TTAACTCTTGATAGCATTGG+TGG + chr2_4:17993942-17993961 Msa0318850:CDS 35.0%
!! TAACTCTTGATAGCATTGGT+GGG + chr2_4:17993943-17993962 Msa0318850:CDS 35.0%
AATTCAAAAACTTTCCGAGC+AGG - chr2_4:17994062-17994081 None:intergenic 35.0%
ATCGCTTTGTAGGATCTTTA+AGG - chr2_4:17994085-17994104 None:intergenic 35.0%
AATATTCTCGAATGTGTCTC+TGG + chr2_4:17994181-17994200 Msa0318850:intron 35.0%
ATGTGTGCAATTTCATGCAT+TGG - chr2_4:17994329-17994348 None:intergenic 35.0%
ATTTCAAATGGACCCTATCA+TGG + chr2_4:17994347-17994366 Msa0318850:CDS 35.0%
CTTATGCCCTAGAAATTGTT+CGG + chr2_4:17994451-17994470 Msa0318850:intron 35.0%
!! ATTATGGATGTGGCTTTAAG+GGG + chr2_4:17994629-17994648 Msa0318850:CDS 35.0%
ACAAAACCATTTGGAAGGAT+TGG - chr2_4:17994674-17994693 None:intergenic 35.0%
! ATGGTGCATTAAGATTTGGT+TGG - chr2_4:17994712-17994731 None:intergenic 35.0%
CCAAATGGTGCATTAAGATT+TGG - chr2_4:17994716-17994735 None:intergenic 35.0%
CCAAATCTTAATGCACCATT+TGG + chr2_4:17994713-17994732 Msa0318850:CDS 35.0%
TAGAATAGTAGCTGCCAATT+GGG - chr2_4:17994855-17994874 None:intergenic 35.0%
TTAGAATAGTAGCTGCCAAT+TGG - chr2_4:17994856-17994875 None:intergenic 35.0%
! ATGGATTACTCAATGGAAGG+TGG + chr2_4:17990043-17990062 Msa0318850:CDS 40.0%
GAGAATTCAAGAGAAGGTCA+CGG - chr2_4:17990369-17990388 None:intergenic 40.0%
! TCTCGTGAGTTTAGCTCAAT+TGG - chr2_4:17990601-17990620 None:intergenic 40.0%
GTCAATTGGCTGTGAAAGTT+GGG + chr2_4:17991216-17991235 Msa0318850:CDS 40.0%
GAACAAGAGAACTCTGTTGA+AGG + chr2_4:17991271-17991290 Msa0318850:CDS 40.0%
!! AAATGTTGATGGTCTTCCAC+AGG - chr2_4:17991979-17991998 None:intergenic 40.0%
AAATGCTCAGCTGAAACAAG+AGG + chr2_4:17992020-17992039 Msa0318850:CDS 40.0%
! TCTGCATGTAACTTGCAAGA+AGG - chr2_4:17992170-17992189 None:intergenic 40.0%
GCAGAAAGAGATAACACTAC+CGG + chr2_4:17992185-17992204 Msa0318850:CDS 40.0%
! TTAAGAGCTTGCTCAAACTC+CGG - chr2_4:17992207-17992226 None:intergenic 40.0%
TGAAATTCCACCAAACCGAA+CGG + chr2_4:17992260-17992279 Msa0318850:CDS 40.0%
TTGAGCACTCATAAACCGTT+CGG - chr2_4:17992278-17992297 None:intergenic 40.0%
GAGTGCTCAAATCATGTTCA+TGG + chr2_4:17992288-17992307 Msa0318850:CDS 40.0%
! TCAAGTTGTAGATGCTGCAA+TGG + chr2_4:17992386-17992405 Msa0318850:CDS 40.0%
AGATGCTGCAATGGATGAAT+TGG + chr2_4:17992395-17992414 Msa0318850:CDS 40.0%
CTGCAATGGATGAATTGGTT+AGG + chr2_4:17992400-17992419 Msa0318850:CDS 40.0%
!!! TGAGAGTTGATGAGCCTTTT+TGG + chr2_4:17992427-17992446 Msa0318850:CDS 40.0%
!!! GAGAGTTGATGAGCCTTTTT+GGG + chr2_4:17992428-17992447 Msa0318850:CDS 40.0%
GTGTACCCATCTTGAGTATT+TGG - chr2_4:17992462-17992481 None:intergenic 40.0%
! TCAGGATTGGTGAAGATTAG+TGG + chr2_4:17992567-17992586 Msa0318850:CDS 40.0%
!! TCGAGATGGAGCTTTATTAC+TGG + chr2_4:17992929-17992948 Msa0318850:CDS 40.0%
CGAATGATGTTAAATTCGCG+AGG - chr2_4:17993091-17993110 None:intergenic 40.0%
!! TACTGCAGAAAAGTTGATGC+TGG + chr2_4:17993112-17993131 Msa0318850:CDS 40.0%
!! GAAAAGTTGATGCTGGTGTA+TGG + chr2_4:17993119-17993138 Msa0318850:CDS 40.0%
ATCATTCGTGAAATGCCTCA+TGG + chr2_4:17993223-17993242 Msa0318850:CDS 40.0%
TCATTCGTGAAATGCCTCAT+GGG + chr2_4:17993224-17993243 Msa0318850:CDS 40.0%
ATTTATACGGAGCTGAAAGC+TGG + chr2_4:17993601-17993620 Msa0318850:CDS 40.0%
TCAGTGATCCAAACACTTGA+AGG + chr2_4:17993831-17993850 Msa0318850:intron 40.0%
CAGTGATCCAAACACTTGAA+GGG + chr2_4:17993832-17993851 Msa0318850:intron 40.0%
TATTCCTCCCTTCAAGTGTT+TGG - chr2_4:17993842-17993861 None:intergenic 40.0%
TGTGAATGCTTAACAATGCC+TGG + chr2_4:17993900-17993919 Msa0318850:CDS 40.0%
!! AACTCTTGATAGCATTGGTG+GGG + chr2_4:17993944-17993963 Msa0318850:CDS 40.0%
TAGTGGGATGGTCTTTCTTA+TGG + chr2_4:17994299-17994318 Msa0318850:intron 40.0%
GAAATGCAGTTGCCATGATA+GGG - chr2_4:17994362-17994381 None:intergenic 40.0%
TGAAATGCAGTTGCCATGAT+AGG - chr2_4:17994363-17994382 None:intergenic 40.0%
GAAAGTTTCACTTCTCCTGT+AGG + chr2_4:17994572-17994591 Msa0318850:CDS 40.0%
GAGTTACCAATCCTTCCAAA+TGG + chr2_4:17994665-17994684 Msa0318850:CDS 40.0%
ATACTGTCAGATGCTCCAAA+TGG - chr2_4:17994731-17994750 None:intergenic 40.0%
! GCATCTGACAGTATTGAAGA+TGG + chr2_4:17994737-17994756 Msa0318850:CDS 40.0%
AGATGGCTCGATGTTAACAT+TGG + chr2_4:17994754-17994773 Msa0318850:CDS 40.0%
AGTTGACCAAGTGCTTAATG+TGG + chr2_4:17994808-17994827 Msa0318850:CDS 40.0%
CATCTTCCACATTAAGCACT+TGG - chr2_4:17994817-17994836 None:intergenic 40.0%
ATGTGAAGGATGCTCTTATG+AGG + chr2_4:17994876-17994895 Msa0318850:CDS 40.0%
GATTACTCAATGGAAGGTGG+CGG + chr2_4:17990046-17990065 Msa0318850:CDS 45.0%
GAGGTAAGACGTTTGTAGGA+AGG - chr2_4:17990121-17990140 None:intergenic 45.0%
AAGTATGGATGTTTGAGCGG+AGG - chr2_4:17990140-17990159 None:intergenic 45.0%
CGTCAATTGGCTGTGAAAGT+TGG + chr2_4:17991215-17991234 Msa0318850:CDS 45.0%
ATGTGGACGTGTCAGAATCA+CGG - chr2_4:17991731-17991750 None:intergenic 45.0%
AGAAAAACGGTGCTCTTCGT+CGG + chr2_4:17991866-17991885 Msa0318850:CDS 45.0%
CCACAATTTGCGAAGCTTGT+GGG + chr2_4:17991941-17991960 Msa0318850:CDS 45.0%
CACAATTTGCGAAGCTTGTG+GGG + chr2_4:17991942-17991961 Msa0318850:CDS 45.0%
ACAATTTGCGAAGCTTGTGG+GGG + chr2_4:17991943-17991962 Msa0318850:CDS 45.0%
TGATGGTCTTCCACAGGAAA+TGG - chr2_4:17991973-17991992 None:intergenic 45.0%
AGACCATCAACATTTCGTGC+AGG + chr2_4:17991984-17992003 Msa0318850:CDS 45.0%
TCTCCTGCACGAAATGTTGA+TGG - chr2_4:17991990-17992009 None:intergenic 45.0%
!! TCATAAACCGTTCGGTTTGG+TGG - chr2_4:17992270-17992289 None:intergenic 45.0%
CACTCATAAACCGTTCGGTT+TGG - chr2_4:17992273-17992292 None:intergenic 45.0%
! ACTATAAGGAGAGGTGTGCT+AGG + chr2_4:17992318-17992337 Msa0318850:CDS 45.0%
! CTATAAGGAGAGGTGTGCTA+GGG + chr2_4:17992319-17992338 Msa0318850:CDS 45.0%
TGTTTCCAACCACGAGAAAG+AGG - chr2_4:17993190-17993209 None:intergenic 45.0%
TCACGAATGATGCATCCAGA+GGG - chr2_4:17993214-17993233 None:intergenic 45.0%
TTCACGAATGATGCATCCAG+AGG - chr2_4:17993215-17993234 None:intergenic 45.0%
CATTCGTGAAATGCCTCATG+GGG + chr2_4:17993225-17993244 Msa0318850:CDS 45.0%
ATTCGTGAAATGCCTCATGG+GGG + chr2_4:17993226-17993245 Msa0318850:CDS 45.0%
!! TTGGGTTGAACATGTGGAAG+TGG + chr2_4:17993527-17993546 Msa0318850:CDS 45.0%
CCTCCTATGGTTTCTTCAAC+AGG - chr2_4:17993684-17993703 None:intergenic 45.0%
CCTGTTGAAGAAACCATAGG+AGG + chr2_4:17993681-17993700 Msa0318850:CDS 45.0%
TGATCCAAACACTTGAAGGG+AGG + chr2_4:17993835-17993854 Msa0318850:CDS 45.0%
CTTAACAATGCCTGGTCAAG+TGG + chr2_4:17993908-17993927 Msa0318850:CDS 45.0%
TGGTTAAACTCCACTTGACC+AGG - chr2_4:17993921-17993940 None:intergenic 45.0%
GATGATACAAGCCAGCCAAA+TGG + chr2_4:17993996-17994015 Msa0318850:CDS 45.0%
!! TAACAACAGTGCCATTTGGC+TGG - chr2_4:17994010-17994029 None:intergenic 45.0%
! GCAGTAACAACAGTGCCATT+TGG - chr2_4:17994014-17994033 None:intergenic 45.0%
TTACTGCTGCTACCACCATT+TGG + chr2_4:17994027-17994046 Msa0318850:CDS 45.0%
AGATCCTACAAAGCGATCAC+AGG + chr2_4:17994088-17994107 Msa0318850:CDS 45.0%
GTTACCTGTGATCGCTTTGT+AGG - chr2_4:17994095-17994114 None:intergenic 45.0%
ATTCTCGAATGTGTCTCTGG+TGG + chr2_4:17994184-17994203 Msa0318850:intron 45.0%
TCTGAGTAGGGATGAATGAC+TGG - chr2_4:17994532-17994551 None:intergenic 45.0%
AGTCATTCATCCCTACTCAG+AGG + chr2_4:17994531-17994550 Msa0318850:intron 45.0%
TCACCATTTGCCTCTGAGTA+GGG - chr2_4:17994544-17994563 None:intergenic 45.0%
ATCACCATTTGCCTCTGAGT+AGG - chr2_4:17994545-17994564 None:intergenic 45.0%
!! TGGACTGGTGCTCAGAATTT+GGG - chr2_4:17994783-17994802 None:intergenic 45.0%
!! ATGGACTGGTGCTCAGAATT+TGG - chr2_4:17994784-17994803 None:intergenic 45.0%
TTGGTCAACTGCATATGGAC+TGG - chr2_4:17994798-17994817 None:intergenic 45.0%
AGCACTTGGTCAACTGCATA+TGG - chr2_4:17994803-17994822 None:intergenic 45.0%
AGCGGAGGTAAGACGTTTGT+AGG - chr2_4:17990125-17990144 None:intergenic 50.0%
CAGAGAACCCATCGCTGTTT+CGG + chr2_4:17990419-17990438 Msa0318850:intron 50.0%
ATCACAGCCGAAACAGCGAT+GGG - chr2_4:17990429-17990448 None:intergenic 50.0%
TATCACAGCCGAAACAGCGA+TGG - chr2_4:17990430-17990449 None:intergenic 50.0%
GAAAAACGGTGCTCTTCGTC+GGG + chr2_4:17991867-17991886 Msa0318850:CDS 50.0%
GCCACAATTTGCGAAGCTTG+TGG + chr2_4:17991940-17991959 Msa0318850:CDS 50.0%
CCCACAAGCTTCGCAAATTG+TGG - chr2_4:17991944-17991963 None:intergenic 50.0%
TGGTCTTCCACAGGAAATGG+TGG - chr2_4:17991970-17991989 None:intergenic 50.0%
GGTTGGCATGTTTCTCGACT+CGG + chr2_4:17992599-17992618 Msa0318850:CDS 50.0%
!! GTGTTTGAAATCGGTTCGCC+AGG + chr2_4:17992903-17992922 Msa0318850:CDS 50.0%
AAAGCTCCATCTCGACTTCC+TGG - chr2_4:17992924-17992943 None:intergenic 50.0%
! CGAGAAAGAGGTGCAGTGTT+TGG - chr2_4:17993178-17993197 None:intergenic 50.0%
AACACTGCACCTCTTTCTCG+TGG + chr2_4:17993178-17993197 Msa0318850:CDS 50.0%
!! GGTCACTTGGGTTGAACATG+TGG + chr2_4:17993521-17993540 Msa0318850:intron 50.0%
CGGAGCTGAAAGCTGGATTA+AGG + chr2_4:17993608-17993627 Msa0318850:CDS 50.0%
!! TGGCTGGCTTGTATCATCGT+CGG - chr2_4:17993994-17994013 None:intergenic 50.0%
AAACTTTCCGAGCAGGCAGA+GGG - chr2_4:17994055-17994074 None:intergenic 50.0%
AAAACTTTCCGAGCAGGCAG+AGG - chr2_4:17994056-17994075 None:intergenic 50.0%
! TGTGTCTCTGGTGGTGACTT+TGG + chr2_4:17994193-17994212 Msa0318850:intron 50.0%
CATCCCTACTCAGAGGCAAA+TGG + chr2_4:17994538-17994557 Msa0318850:CDS 50.0%
! TTCTTTTGCTCCATCCCCGC+AGG + chr2_4:17990394-17990413 Msa0318850:intron 55.0%
! TCTTTTGCTCCATCCCCGCA+GGG + chr2_4:17990395-17990414 Msa0318850:intron 55.0%
CGAGGCAGAACGACGTCAAT+TGG + chr2_4:17991202-17991221 Msa0318850:CDS 55.0%
AATTCGCGAGGCCTCACTAG+AGG - chr2_4:17993079-17993098 None:intergenic 55.0%
AGGTGCAGTGTTTGGTCGAG+AGG - chr2_4:17993170-17993189 None:intergenic 55.0%
CTGCACCTCTTTCTCGTGGT+TGG + chr2_4:17993182-17993201 Msa0318850:CDS 55.0%
CGTGGTTGGAAACATCCCTC+TGG + chr2_4:17993196-17993215 Msa0318850:CDS 55.0%
AATGCCTCATGGGGGTTGTC+TGG + chr2_4:17993234-17993253 Msa0318850:CDS 55.0%
CTAACCAGACAACCCCCATG+AGG - chr2_4:17993241-17993260 None:intergenic 55.0%
! CTGGCTTGTATCATCGTCGG+TGG - chr2_4:17993991-17994010 None:intergenic 55.0%
! ATGGAAGGTGGCGGTGAGTC+CGG + chr2_4:17990055-17990074 Msa0318850:CDS 60.0%
! CTTTTGCTCCATCCCCGCAG+GGG + chr2_4:17990396-17990415 Msa0318850:intron 60.0%
GGAATGCCACCATCCAGACG+AGG + chr2_4:17991184-17991203 Msa0318850:CDS 60.0%
GTTCTGCCTCGTCTGGATGG+TGG - chr2_4:17991193-17991212 None:intergenic 60.0%
GTCGTTCTGCCTCGTCTGGA+TGG - chr2_4:17991196-17991215 None:intergenic 60.0%
TGACGTCGTTCTGCCTCGTC+TGG - chr2_4:17991200-17991219 None:intergenic 60.0%
TGGGGGTCCACCATTTCCTG+TGG + chr2_4:17991960-17991979 Msa0318850:CDS 60.0%
GGTTCGCCAGGAAGTCGAGA+TGG + chr2_4:17992915-17992934 Msa0318850:CDS 60.0%
AGGCAGAGGGAGCCAAATGG+TGG - chr2_4:17994042-17994061 None:intergenic 60.0%
AGCAGGCAGAGGGAGCCAAA+TGG - chr2_4:17994045-17994064 None:intergenic 60.0%
! TTTGGCTCCCTCTGCCTGCT+CGG + chr2_4:17994045-17994064 Msa0318850:CDS 60.0%
ATGGGTTCTCTGCCCCTGCG+GGG - chr2_4:17990411-17990430 None:intergenic 65.0%
! GATGGGTTCTCTGCCCCTGC+GGG - chr2_4:17990412-17990431 None:intergenic 65.0%
! CGATGGGTTCTCTGCCCCTG+CGG - chr2_4:17990413-17990432 None:intergenic 65.0%
GGTGGTCGGGGTTTGAACCC+TGG + chr2_4:17990547-17990566 Msa0318850:intron 65.0%
GTTCTCTGCCCCTGCGGGGA+TGG - chr2_4:17990407-17990426 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 17990043 17994907 17990043 ID=Msa0318850;Name=Msa0318850
chr2_4 mRNA 17990043 17994907 17990043 ID=Msa0318850-mRNA-1;Parent=Msa0318850;Name=Msa0318850-mRNA-1;_AED=0.29;_eAED=0.29;_QI=0|0|0|1|1|1|10|0|710
chr2_4 exon 17990043 17990164 17990043 ID=Msa0318850-mRNA-1:exon:6738;Parent=Msa0318850-mRNA-1
chr2_4 exon 17991175 17991292 17991175 ID=Msa0318850-mRNA-1:exon:6739;Parent=Msa0318850-mRNA-1
chr2_4 exon 17991850 17992041 17991850 ID=Msa0318850-mRNA-1:exon:6740;Parent=Msa0318850-mRNA-1
chr2_4 exon 17992150 17992620 17992150 ID=Msa0318850-mRNA-1:exon:6741;Parent=Msa0318850-mRNA-1
chr2_4 exon 17992849 17992950 17992849 ID=Msa0318850-mRNA-1:exon:6742;Parent=Msa0318850-mRNA-1
chr2_4 exon 17993049 17993255 17993049 ID=Msa0318850-mRNA-1:exon:6743;Parent=Msa0318850-mRNA-1
chr2_4 exon 17993522 17993702 17993522 ID=Msa0318850-mRNA-1:exon:6744;Parent=Msa0318850-mRNA-1
chr2_4 exon 17993835 17994109 17993835 ID=Msa0318850-mRNA-1:exon:6745;Parent=Msa0318850-mRNA-1
chr2_4 exon 17994302 17994391 17994302 ID=Msa0318850-mRNA-1:exon:6746;Parent=Msa0318850-mRNA-1
chr2_4 exon 17994533 17994907 17994533 ID=Msa0318850-mRNA-1:exon:6747;Parent=Msa0318850-mRNA-1
chr2_4 CDS 17990043 17990164 17990043 ID=Msa0318850-mRNA-1:cds;Parent=Msa0318850-mRNA-1
chr2_4 CDS 17991175 17991292 17991175 ID=Msa0318850-mRNA-1:cds;Parent=Msa0318850-mRNA-1
chr2_4 CDS 17991850 17992041 17991850 ID=Msa0318850-mRNA-1:cds;Parent=Msa0318850-mRNA-1
chr2_4 CDS 17992150 17992620 17992150 ID=Msa0318850-mRNA-1:cds;Parent=Msa0318850-mRNA-1
chr2_4 CDS 17992849 17992950 17992849 ID=Msa0318850-mRNA-1:cds;Parent=Msa0318850-mRNA-1
chr2_4 CDS 17993049 17993255 17993049 ID=Msa0318850-mRNA-1:cds;Parent=Msa0318850-mRNA-1
chr2_4 CDS 17993522 17993702 17993522 ID=Msa0318850-mRNA-1:cds;Parent=Msa0318850-mRNA-1
chr2_4 CDS 17993835 17994109 17993835 ID=Msa0318850-mRNA-1:cds;Parent=Msa0318850-mRNA-1
chr2_4 CDS 17994302 17994391 17994302 ID=Msa0318850-mRNA-1:cds;Parent=Msa0318850-mRNA-1
chr2_4 CDS 17994533 17994907 17994533 ID=Msa0318850-mRNA-1:cds;Parent=Msa0318850-mRNA-1
Gene Sequence

>Msa0318850

ATGGATTACTCAATGGAAGGTGGCGGTGAGTCCGGTGATGAAGACATTAGTAACTTTCGAGATGATGGAAACAAACCTTCCTACAAACGTCTTACCTCCGCTCAAACATCCATACTTGAAAATTTCATGAAGGAATGCCACCATCCAGACGAGGCAGAACGACGTCAATTGGCTGTGAAAGTTGGGCTTGAGCCCAAACAAATCAAGTTTTGGTTTCAGAACAAGAGAACTCTGTTGAAGCATCAACATGAACGAGAGAAAAACGGTGCTCTTCGTCGGGAGAATGACAAGATTCGTAACGAAAATCTCAAAATAAAAGAGGTACTGAAAGCCACAATTTGCGAAGCTTGTGGGGGTCCACCATTTCCTGTGGAAGACCATCAACATTTCGTGCAGGAGATGCAGCAAGAAAATGCTCAGCTGAAACAAGAGTGTGAAAAAATGTCTAGCCTTCTTGCAAGTTACATGCAGAAAGAGATAACACTACCGGAGTTTGAGCAAGCTCTTAAGTCTATAAAATCATTTTCACGCGACAGAGAACTTGAAATTCCACCAAACCGAACGGTTTATGAGTGCTCAAATCATGTTCATGGTAATCTAACTATAAGGAGAGGTGTGCTAGGGCTCAACTTAACACAAAACTATGATGCACAAAAGATAACTATGTCTCAAGTTGTAGATGCTGCAATGGATGAATTGGTTAGGCTTGTGAGAGTTGATGAGCCTTTTTGGGTCAAATCTCCAAATACTCAAGATGGGTACACTCTTCATCGCGAAAGTTATGAGCAAGTTTTTCCAAAGAACAATCACTTTAAAGGTGCGTATGTGTGCGAAGAATCTTCCAAATATTCAGGATTGGTGAAGATTAGTGGCATAGATTTGGTTGGCATGTTTCTCGACTCGGTCAAATGGACAAACTTGTTCCCTACAATTGTTACCAAAGCTGAAACTATCAAAGTGTTTGAAATCGGTTCGCCAGGAAGTCGAGATGGAGCTTTATTACTGATGAATGAAGAAATGCATATTTTATCGCCTCTAGTGAGGCCTCGCGAATTTAACATCATTCGCTACTGCAGAAAAGTTGATGCTGGTGTATGGGTGATAACTGATATCTCATTTGACTCCTCTCGACCAAACACTGCACCTCTTTCTCGTGGTTGGAAACATCCCTCTGGATGCATCATTCGTGAAATGCCTCATGGGGGTTGTCTGGTCACTTGGGTTGAACATGTGGAAGTGGAAGATAAGATCCATACACATTACATGTATAGAGATCTTGTTGGCGACTATAATTTATACGGAGCTGAAAGCTGGATTAAGGAGCTTCAAAGAATGTGTGAGAGATCTTTAGGCTCTTATGTTAAAGCAATACCTGTTGAAGAAACCATAGGAGTGATCCAAACACTTGAAGGGAGGAATAGTGTGATAAAGCTTGCTGATAGAATGGTCAAAATGTTTTGTGAATGCTTAACAATGCCTGGTCAAGTGGAGTTTAACCATTTAACTCTTGATAGCATTGGTGGGGTTAGAGTCTCTCTTCGCAATACCACCGACGATGATACAAGCCAGCCAAATGGCACTGTTGTTACTGCTGCTACCACCATTTGGCTCCCTCTGCCTGCTCGGAAAGTTTTTGAATTCCTTAAAGATCCTACAAAGCGATCACAGTGGGATGGTCTTTCTTATGGAAATCCAATGCATGAAATTGCACACATTTCAAATGGACCCTATCATGGCAACTGCATTTCAATTATCAAGTCATTCATCCCTACTCAGAGGCAAATGGTGATTCTACAAGAAAGTTTCACTTCTCCTGTAGGATCATACATTATATATGCTCCAACTGATAGAAAAATTATGGATGTGGCTTTAAGGGGTGAAGACTGCAAAGAGTTACCAATCCTTCCAAATGGTTTTGTTATTTGTTCGAAGAGCCAACCAAATCTTAATGCACCATTTGGAGCATCTGACAGTATTGAAGATGGCTCGATGTTAACATTGGCAGCCCAAATTCTGAGCACCAGTCCATATGCAGTTGACCAAGTGCTTAATGTGGAAGATGTTACTTATATCAACACCCAATTGGCAGCTACTATTCTAAATGTGAAGGATGCTCTTATGAGGTCTATTTAA
Protein sequence

>Msa0318850

MDYSMEGGGESGDEDISNFRDDGNKPSYKRLTSAQTSILENFMKECHHPDEAERRQLAVKVGLEPKQIKFWFQNKRTLLKHQHEREKNGALRRENDKIRNENLKIKEVLKATICEACGGPPFPVEDHQHFVQEMQQENAQLKQECEKMSSLLASYMQKEITLPEFEQALKSIKSFSRDRELEIPPNRTVYECSNHVHGNLTIRRGVLGLNLTQNYDAQKITMSQVVDAAMDELVRLVRVDEPFWVKSPNTQDGYTLHRESYEQVFPKNNHFKGAYVCEESSKYSGLVKISGIDLVGMFLDSVKWTNLFPTIVTKAETIKVFEIGSPGSRDGALLLMNEEMHILSPLVRPREFNIIRYCRKVDAGVWVITDISFDSSRPNTAPLSRGWKHPSGCIIREMPHGGCLVTWVEHVEVEDKIHTHYMYRDLVGDYNLYGAESWIKELQRMCERSLGSYVKAIPVEETIGVIQTLEGRNSVIKLADRMVKMFCECLTMPGQVEFNHLTLDSIGGVRVSLRNTTDDDTSQPNGTVVTAATTIWLPLPARKVFEFLKDPTKRSQWDGLSYGNPMHEIAHISNGPYHGNCISIIKSFIPTQRQMVILQESFTSPVGSYIIYAPTDRKIMDVALRGEDCKELPILPNGFVICSKSQPNLNAPFGASDSIEDGSMLTLAAQILSTSPYAVDQVLNVEDVTYINTQLAATILNVKDALMRSI*