AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0319460

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0319460 MtrunA17_Chr2g0293901 79.191 173 33 1 1 173 1 170 3.63e-63 204
Msa0319460 MtrunA17_Chr6g0470151 46.113 373 167 9 1 359 1 353 1.01e-59 200
Msa0319460 MtrunA17_Chr2g0289181 43.777 233 51 3 323 475 1 233 2.46e-55 184
Msa0319460 MtrunA17_Chr8g0366921 44.848 330 159 8 1 317 1 320 3.26e-55 187
Msa0319460 MtrunA17_Chr7g0266621 48.742 318 144 5 1 315 1 302 1.95e-48 168
Msa0319460 MtrunA17_Chr8g0368721 51.562 320 144 6 1 317 1 312 1.09e-44 159
Msa0319460 MtrunA17_Chr1g0202461 50.000 98 47 2 313 410 239 334 2.54e-25 107
Msa0319460 MtrunA17_Chr8g0348311 47.959 98 48 2 313 410 134 228 1.44e-22 98.6
Msa0319460 MtrunA17_Chr7g0248691 47.059 102 51 2 311 412 131 229 8.25e-22 96.3
Msa0319460 MtrunA17_Chr6g0470161 55.072 69 31 0 407 475 52 120 1.60e-20 87.0
Msa0319460 MtrunA17_Chr2g0293481 39.669 121 61 3 301 410 221 340 6.91e-20 92.0
Msa0319460 MtrunA17_Chr4g0053281 42.593 108 55 1 293 393 216 323 1.65e-19 89.7
Msa0319460 MtrunA17_Chr4g0071521 45.783 83 42 1 314 393 220 302 1.83e-19 89.0
Msa0319460 MtrunA17_Chr8g0387501 40.351 114 66 2 337 450 130 241 2.64e-19 87.8
Msa0319460 MtrunA17_Chr8g0379461 46.429 84 38 1 336 419 76 152 5.46e-19 86.3
Msa0319460 MtrunA17_Chr5g0401821 45.783 83 42 1 314 393 174 256 6.08e-19 86.7
Msa0319460 MtrunA17_Chr7g0270051 40.171 117 57 2 287 396 3 113 9.94e-19 85.9
Msa0319460 MtrunA17_Chr2g0319961 57.377 61 26 0 336 396 80 140 3.96e-18 84.0
Msa0319460 MtrunA17_Chr2g0284321 55.738 61 27 0 336 396 35 95 4.65e-18 82.8
Msa0319460 MtrunA17_Chr1g0184691 57.627 59 25 0 337 395 294 352 8.49e-18 84.7
Msa0319460 MtrunA17_Chr7g0228941 50.769 65 32 0 330 394 46 110 2.04e-17 78.6
Msa0319460 MtrunA17_Chr1g0147881 52.459 61 29 0 333 393 259 319 4.66e-17 82.4
Msa0319460 MtrunA17_Chr3g0139021 40.230 87 51 1 308 393 285 371 1.12e-16 82.0
Msa0319460 MtrunA17_Chr4g0074001 46.250 80 41 2 318 396 340 418 1.53e-16 82.0
Msa0319460 MtrunA17_Chr7g0263531 52.632 76 34 2 332 407 185 258 6.95e-16 79.7
Msa0319460 MtrunA17_Chr7g0263531 42.424 66 37 1 331 396 356 420 7.57e-14 73.6
Msa0319460 MtrunA17_Chr7g0218081 50.000 66 32 1 331 396 405 469 2.61e-15 78.2
Msa0319460 MtrunA17_Chr7g0218081 46.970 66 33 2 332 397 224 287 3.87e-13 71.6
Msa0319460 MtrunA17_Chr5g0445311 43.056 72 40 1 325 396 353 423 7.30e-15 77.0
Msa0319460 MtrunA17_Chr5g0445311 44.444 72 38 2 335 406 199 268 4.41e-12 68.2
Msa0319460 MtrunA17_Chr3g0079691 42.391 92 47 4 335 424 279 366 1.94e-14 75.9
Msa0319460 MtrunA17_Chr3g0079691 42.424 66 37 1 331 396 490 554 3.99e-12 68.6
Msa0319460 MtrunA17_Chr3g0101131 41.667 72 41 1 325 396 404 474 3.42e-14 75.1
Msa0319460 MtrunA17_Chr3g0101131 45.946 74 38 2 332 405 239 310 5.12e-13 71.2
Msa0319460 MtrunA17_Chr2g0302391 44.615 65 36 0 336 400 211 275 5.44e-14 73.9
Msa0319460 MtrunA17_Chr5g0447701 48.485 66 33 1 331 396 247 311 6.12e-14 73.6
Msa0319460 MtrunA17_Chr3g0099641 31.387 137 83 2 277 413 203 328 1.14e-13 72.8
Msa0319460 MtrunA17_Chr5g0418191 45.455 66 35 1 331 396 315 379 1.26e-13 72.8
Msa0319460 MtrunA17_Chr5g0418191 49.231 65 31 2 332 396 147 209 1.09e-11 66.6
Msa0319460 MtrunA17_Chr3g0136961 45.614 57 31 0 337 393 139 195 1.71e-13 70.9
Msa0319460 MtrunA17_Chr5g0432161 48.333 60 30 1 337 396 422 480 2.14e-13 72.4
Msa0319460 MtrunA17_Chr5g0432161 52.381 63 28 2 335 397 243 303 1.93e-12 69.3
Msa0319460 MtrunA17_Chr2g0322391 36.036 111 66 2 303 413 321 426 2.49e-13 72.4
Msa0319460 MtrunA17_Chr4g0075201 31.298 131 81 3 272 396 200 327 3.37e-13 71.6
Msa0319460 MtrunA17_Chr7g0270191 36.170 94 56 3 306 396 112 204 4.92e-13 69.7
Msa0319460 MtrunA17_Chr4g0001361 42.188 64 37 0 337 400 163 226 5.68e-13 69.7
Msa0319460 MtrunA17_Chr7g0249311 30.435 138 78 6 280 401 321 456 7.27e-13 70.9
Msa0319460 MtrunA17_Chr7g0249311 44.000 75 36 3 335 407 178 248 5.12e-11 65.1
Msa0319460 MtrunA17_Chr1g0194481 36.667 90 55 2 316 405 139 226 7.50e-13 69.3
Msa0319460 MtrunA17_Chr7g0238921 40.698 86 45 3 323 408 113 192 8.05e-13 69.3
Msa0319460 MtrunA17_Chr7g0270831 38.462 78 47 1 319 396 200 276 9.38e-13 70.1
Msa0319460 MtrunA17_Chr3g0106491 46.667 60 31 1 337 396 366 424 9.87e-13 70.1
Msa0319460 MtrunA17_Chr3g0106491 51.613 62 28 2 335 396 197 256 4.43e-12 68.2
Msa0319460 MtrunA17_Chr4g0042831 43.750 64 35 1 333 396 275 337 1.23e-12 69.3
Msa0319460 MtrunA17_Chr4g0042831 33.010 103 61 2 298 400 114 208 8.18e-11 63.5
Msa0319460 MtrunA17_Chr4g0025061 36.145 83 53 0 331 413 355 437 1.29e-12 70.1
Msa0319460 MtrunA17_Chr1g0150271 27.778 162 100 4 242 398 14 163 1.71e-12 67.4
Msa0319460 MtrunA17_Chr2g0288871 35.000 100 61 3 298 396 269 365 1.92e-12 69.3
Msa0319460 MtrunA17_Chr7g0249301 47.541 61 29 3 337 396 293 351 3.47e-12 67.8
Msa0319460 MtrunA17_Chr7g0266611 63.077 65 23 1 411 475 102 165 4.21e-12 64.3
Msa0319460 MtrunA17_Chr4g0025051 36.145 83 53 0 331 413 330 412 4.40e-12 68.2
Msa0319460 MtrunA17_Chr1g0150281 43.860 57 32 0 337 393 141 197 6.69e-12 66.2
Msa0319460 MtrunA17_Chr7g0243891 47.143 70 35 2 328 397 208 275 6.72e-12 67.8
Msa0319460 MtrunA17_Chr4g0004911 42.424 66 37 1 331 396 331 395 8.63e-12 67.0
Msa0319460 MtrunA17_Chr3g0129321 40.625 64 37 1 333 396 115 177 2.01e-11 63.5
Msa0319460 MtrunA17_Chr1g0150801 44.262 61 33 1 333 393 99 158 2.21e-11 62.4
Msa0319460 MtrunA17_Chr4g0060271 38.462 78 46 1 321 396 238 315 2.43e-11 65.9
Msa0319460 MtrunA17_Chr2g0294231 45.000 60 32 1 337 396 204 262 2.50e-11 65.1
Msa0319460 MtrunA17_Chr2g0333851 37.805 82 47 1 321 398 150 231 2.79e-11 64.7
Msa0319460 MtrunA17_Chr4g0051881 41.429 70 39 2 332 401 175 242 2.96e-11 64.7
Msa0319460 MtrunA17_Chr7g0216891 31.858 113 72 3 281 393 169 276 5.12e-11 65.1
Msa0319460 MtrunA17_Chr5g0443131 41.538 65 37 1 337 401 158 221 5.13e-11 63.9
Msa0319460 MtrunA17_Chr4g0068541 37.681 69 42 1 337 405 209 276 6.14e-11 64.3
Msa0319460 MtrunA17_Chr5g0418781 35.000 100 63 2 294 393 83 180 7.44e-11 62.8
Msa0319460 MtrunA17_Chr4g0051181 41.667 60 35 0 337 396 244 303 8.89e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0319460 AT5G52830.1 51.020 98 46 2 313 410 143 238 4.39e-25 106
Msa0319460 AT4G01250.1 32.850 207 126 6 276 474 72 273 2.40e-24 103
Msa0319460 AT2G34830.1 42.308 104 49 3 311 405 182 283 1.28e-18 88.6
Msa0319460 AT2G34830.2 42.308 104 49 3 311 405 191 292 1.47e-18 88.2
Msa0319460 AT5G45050.4 44.681 94 50 2 313 405 1017 1109 2.06e-18 89.4
Msa0319460 AT5G45050.1 44.681 94 50 2 313 405 1156 1248 2.09e-18 89.4
Msa0319460 AT5G45050.2 44.681 94 50 2 313 405 1128 1220 2.27e-18 89.0
Msa0319460 AT4G23550.1 41.758 91 52 1 320 410 124 213 2.29e-18 86.3
Msa0319460 AT2G24570.1 53.968 63 29 0 333 395 240 302 2.30e-18 86.3
Msa0319460 AT2G23320.1 57.895 57 24 0 337 393 241 297 2.84e-18 85.9
Msa0319460 AT4G31550.2 55.738 61 27 0 333 393 242 302 2.85e-18 86.3
Msa0319460 AT4G31550.1 55.738 61 27 0 333 393 243 303 2.91e-18 86.3
Msa0319460 AT5G28650.1 47.059 85 42 1 314 395 237 321 3.76e-18 85.9
Msa0319460 AT1G30650.1 42.308 104 49 3 311 405 184 285 3.87e-18 87.0
Msa0319460 AT1G29280.1 45.055 91 41 1 315 396 44 134 5.04e-18 84.3
Msa0319460 AT4G24240.1 45.783 83 42 1 314 393 256 338 7.21e-18 85.5
Msa0319460 AT3G04670.1 47.059 85 42 1 314 395 237 321 7.44e-18 85.1
Msa0319460 AT3G58710.1 54.098 61 28 0 336 396 71 131 4.82e-17 81.6
Msa0319460 AT3G58710.2 54.098 61 28 0 336 396 70 130 5.37e-17 81.3
Msa0319460 AT5G07100.1 35.294 136 80 5 264 396 163 293 2.09e-16 80.5
Msa0319460 AT5G07100.1 43.056 72 40 1 329 400 110 180 1.62e-11 65.9
Msa0319460 AT4G30935.1 40.000 105 58 3 293 396 290 390 2.26e-16 81.6
Msa0319460 AT2G30590.1 55.932 59 26 0 337 395 314 372 3.90e-16 80.5
Msa0319460 AT5G07100.2 35.294 136 80 5 264 396 70 200 4.08e-16 77.8
Msa0319460 AT5G07100.2 42.466 73 41 1 329 401 17 88 3.88e-11 63.2
Msa0319460 AT5G07100.4 35.294 136 80 5 264 396 70 200 4.08e-16 77.8
Msa0319460 AT5G07100.4 42.466 73 41 1 329 401 17 88 3.88e-11 63.2
Msa0319460 AT5G07100.5 35.294 136 80 5 264 396 70 200 4.08e-16 77.8
Msa0319460 AT5G07100.5 42.466 73 41 1 329 401 17 88 3.88e-11 63.2
Msa0319460 AT5G07100.3 35.294 136 80 5 264 396 70 200 4.08e-16 77.8
Msa0319460 AT5G07100.3 42.466 73 41 1 329 401 17 88 3.88e-11 63.2
Msa0319460 AT3G01080.2 48.485 66 33 1 331 396 339 403 4.67e-15 77.8
Msa0319460 AT3G01080.2 45.714 70 36 2 332 401 201 268 1.18e-13 73.2
Msa0319460 AT2G04880.1 35.652 115 60 2 295 396 253 366 4.87e-15 77.8
Msa0319460 AT2G04880.2 36.434 129 62 5 298 412 231 353 5.53e-15 77.4
Msa0319460 AT3G01080.1 48.485 66 33 1 331 396 301 365 5.98e-15 77.0
Msa0319460 AT3G01080.1 45.714 70 36 2 332 401 163 230 1.57e-13 72.8
Msa0319460 AT4G26640.7 41.667 72 41 1 325 396 330 400 3.56e-14 75.1
Msa0319460 AT4G26640.7 34.146 123 71 3 291 413 134 246 1.60e-11 67.0
Msa0319460 AT4G26640.5 41.667 72 41 1 325 396 194 264 3.87e-14 74.3
Msa0319460 AT4G26640.5 41.250 80 45 2 334 413 33 110 4.18e-11 65.1
Msa0319460 AT4G26640.6 41.667 72 41 1 325 396 194 264 3.87e-14 74.3
Msa0319460 AT4G26640.6 41.250 80 45 2 334 413 33 110 4.18e-11 65.1
Msa0319460 AT4G26640.4 41.667 72 41 1 325 396 194 264 3.87e-14 74.3
Msa0319460 AT4G26640.4 41.250 80 45 2 334 413 33 110 4.18e-11 65.1
Msa0319460 AT4G26640.3 41.667 72 41 1 325 396 194 264 3.87e-14 74.3
Msa0319460 AT4G26640.3 41.250 80 45 2 334 413 33 110 4.18e-11 65.1
Msa0319460 AT4G26640.2 41.667 72 41 1 325 396 370 440 4.06e-14 75.1
Msa0319460 AT4G26640.2 34.146 123 71 3 291 413 174 286 1.80e-11 66.6
Msa0319460 AT3G01080.3 45.714 70 36 2 332 401 201 268 4.39e-14 73.6
Msa0319460 AT4G26640.1 41.667 72 41 1 325 396 298 368 4.64e-14 74.7
Msa0319460 AT4G26640.1 34.146 123 71 3 291 413 102 214 2.25e-11 66.2
Msa0319460 AT2G37260.1 41.463 82 43 2 316 393 325 405 5.02e-14 74.3
Msa0319460 AT2G37260.1 50.769 65 30 2 332 396 161 223 5.60e-12 68.2
Msa0319460 AT1G69810.2 43.590 78 43 1 331 407 140 217 6.10e-14 73.2
Msa0319460 AT1G69810.1 43.590 78 43 1 331 407 198 275 8.49e-14 73.2
Msa0319460 AT2G37260.3 41.463 82 43 2 316 393 245 325 8.70e-14 73.2
Msa0319460 AT2G37260.3 50.769 65 30 2 332 396 81 143 9.94e-12 66.6
Msa0319460 AT2G37260.2 41.463 82 43 2 316 393 243 323 9.14e-14 72.8
Msa0319460 AT2G37260.2 50.769 65 30 2 332 396 79 141 1.06e-11 66.6
Msa0319460 AT1G13960.1 43.939 66 36 1 331 396 404 468 9.62e-14 73.9
Msa0319460 AT1G13960.1 44.928 69 36 2 332 400 225 291 3.91e-12 68.9
Msa0319460 AT2G03340.1 43.939 66 36 1 331 396 410 474 1.15e-13 73.6
Msa0319460 AT2G03340.1 35.484 124 63 4 332 443 246 364 4.34e-12 68.6
Msa0319460 AT2G38470.1 51.471 68 31 2 330 397 178 243 1.28e-13 73.6
Msa0319460 AT2G38470.1 46.667 60 31 1 337 396 363 421 3.67e-13 72.0
Msa0319460 AT1G13960.2 43.939 66 36 1 331 396 251 315 2.21e-13 72.0
Msa0319460 AT1G13960.2 44.928 69 36 2 332 400 72 138 1.04e-11 66.6
Msa0319460 AT5G26170.1 47.619 63 32 1 331 393 108 169 4.48e-13 67.8
Msa0319460 AT4G04450.2 36.145 83 53 0 331 413 193 275 8.24e-13 70.5
Msa0319460 AT4G22070.4 39.394 66 40 0 331 396 292 357 1.42e-12 70.1
Msa0319460 AT4G22070.3 39.394 66 40 0 331 396 292 357 1.42e-12 70.1
Msa0319460 AT4G22070.2 39.394 66 40 0 331 396 292 357 1.42e-12 70.1
Msa0319460 AT4G22070.1 39.394 66 40 0 331 396 292 357 1.42e-12 70.1
Msa0319460 AT2G30250.1 45.312 64 34 1 337 400 329 391 1.46e-12 69.7
Msa0319460 AT4G04450.1 36.145 83 53 0 331 413 287 369 1.80e-12 69.7
Msa0319460 AT1G80840.1 37.805 82 47 1 322 399 128 209 1.83e-12 68.6
Msa0319460 AT4G26440.1 38.542 96 53 3 324 419 170 259 2.15e-12 69.7
Msa0319460 AT2G47260.1 36.667 90 55 2 307 396 146 233 3.36e-12 68.2
Msa0319460 AT1G62300.1 40.984 61 36 0 336 396 312 372 4.61e-12 68.6
Msa0319460 AT5G43290.1 51.667 60 28 1 334 393 112 170 8.07e-12 66.2
Msa0319460 AT1G69310.4 37.805 82 49 2 313 394 125 204 1.32e-11 65.9
Msa0319460 AT1G69310.2 37.805 82 49 2 313 394 125 204 1.32e-11 65.9
Msa0319460 AT1G69310.1 37.805 82 49 2 313 394 125 204 1.32e-11 65.9
Msa0319460 AT1G55600.1 38.356 73 43 2 325 396 295 366 1.53e-11 67.0
Msa0319460 AT5G56270.1 48.387 62 30 2 335 396 272 331 1.58e-11 67.0
Msa0319460 AT5G56270.1 43.333 60 33 1 337 396 488 546 1.77e-11 67.0
Msa0319460 AT4G18170.1 29.365 126 82 3 276 401 118 236 2.50e-11 65.1
Msa0319460 AT4G31800.2 40.000 60 36 0 337 396 112 171 2.99e-11 63.9
Msa0319460 AT4G31800.1 40.000 60 36 0 337 396 177 236 4.13e-11 64.3
Msa0319460 AT4G12020.5 33.333 129 68 6 332 448 397 519 4.52e-11 66.2
Msa0319460 AT1G29860.1 38.272 81 47 2 326 404 124 203 5.40e-11 63.9
Msa0319460 AT5G49520.1 37.179 78 47 2 319 396 205 280 5.88e-11 64.7
Msa0319460 AT4G12020.6 33.333 129 68 6 332 448 464 586 6.04e-11 65.9
Msa0319460 AT4G12020.8 33.333 129 68 6 332 448 464 586 6.04e-11 65.9
Msa0319460 AT4G12020.4 33.333 129 68 6 332 448 464 586 6.10e-11 65.9
Msa0319460 AT4G12020.7 33.333 129 68 6 332 448 464 586 6.10e-11 65.9
Msa0319460 AT4G12020.10 33.333 129 68 6 332 448 464 586 6.20e-11 65.9
Msa0319460 AT4G12020.1 33.333 129 68 6 332 448 464 586 6.60e-11 65.5
Msa0319460 AT4G12020.9 33.333 129 68 6 332 448 464 586 6.60e-11 65.5
Msa0319460 AT1G18860.1 43.333 60 34 0 337 396 240 299 6.89e-11 64.7
Msa0319460 AT3G62340.1 40.000 85 47 3 313 396 96 177 7.04e-11 63.5
Msa0319460 AT4G12020.3 33.333 129 68 6 332 448 464 586 7.06e-11 65.5
Msa0319460 AT4G12020.2 33.333 129 68 6 332 448 464 586 7.13e-11 65.5
Msa0319460 AT5G15130.2 37.662 77 46 1 322 396 169 245 7.57e-11 64.7
Msa0319460 AT5G41570.1 31.183 93 61 2 303 393 63 154 8.45e-11 61.2

Find 112 sgRNAs with CRISPR-Local

Find 188 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTCTGAAACAGAATTTAT+TGG 0.120089 2_4:-19190696 Msa0319460:CDS
ATAACACACCAAGGACATTT+TGG 0.248072 2_4:+19192050 None:intergenic
TAGAAACCCAAACTAGTTCT+AGG 0.250090 2_4:-19192266 Msa0319460:CDS
ACAAAGTTCTTAATTTCTTC+TGG 0.267372 2_4:+19190810 None:intergenic
GTCGGGGTGGTGGTGGTTGA+AGG 0.269792 2_4:+19192801 None:intergenic
GTAAAACCCTAGAACTAGTT+TGG 0.270284 2_4:+19192259 None:intergenic
GGGGATAATGGTGGTGGTGG+TGG 0.286343 2_4:+19192827 None:intergenic
AAGGTGGTGGTAGTGGTAAT+TGG 0.290628 2_4:+19192576 None:intergenic
CTAGTTTGGGTTTCTAGAAT+TGG 0.312695 2_4:+19192273 None:intergenic
CAAACCTCAGATCAAGAAAA+AGG 0.317679 2_4:-19192215 Msa0319460:intron
GGTTGAAGGGGTGGGGATAA+TGG 0.324698 2_4:+19192815 None:intergenic
GGTGGTGGTGGTTGAAGGGG+TGG 0.333560 2_4:+19192806 None:intergenic
AGGTGGTGGTAGTGGTAATT+GGG 0.334105 2_4:+19192577 None:intergenic
GGTAATTGGGGTAATGATAG+TGG 0.346701 2_4:+19192590 None:intergenic
AGGGATAATTGTGGTGGGAT+TGG 0.353569 2_4:+19192884 None:intergenic
ATACGGGTGCTAGCGGGGTT+AGG 0.359448 2_4:+19192864 None:intergenic
TGGTGGTGGTTGAAGGGGTG+GGG 0.363221 2_4:+19192808 None:intergenic
CGGGGTGGTGGTGGTTGAAG+GGG 0.364013 2_4:+19192803 None:intergenic
CTTCAATGGATAATGATGAT+TGG 0.367851 2_4:-19193148 None:intergenic
TATTATTAGTGATGTGGGTC+GGG 0.369132 2_4:+19192784 None:intergenic
TTCAATGGATAATGATGATT+GGG 0.373937 2_4:-19193147 None:intergenic
GGGAATGTGAATAGCGGTGG+TGG 0.376751 2_4:+19192635 None:intergenic
GCTTATATTGTGACGTATAA+AGG 0.379319 2_4:-19190852 Msa0319460:CDS
GTGGTGGTGGTTAGGATTTG+AGG 0.379807 2_4:+19193047 None:intergenic
GATTGTTGCTGATTTGTGTT+TGG 0.383802 2_4:+19192237 None:intergenic
GTGGTGGTGGTTGAAGGGGT+GGG 0.384113 2_4:+19192807 None:intergenic
TCTAACTCTATGAAGTCATT+TGG 0.386725 2_4:+19192924 None:intergenic
TACTGATGGTGGTGGTGGTT+AGG 0.393621 2_4:+19193039 None:intergenic
TCTGACATAGCTGTTATGTT+TGG 0.394725 2_4:+19190642 None:intergenic
AGTGATGTGGGTCGGGGTGG+TGG 0.395367 2_4:+19192791 None:intergenic
TAAAACCCTAGAACTAGTTT+GGG 0.406708 2_4:+19192260 None:intergenic
GGTGGGGATAATGGTGGTGG+TGG 0.411568 2_4:+19192824 None:intergenic
AATTGTGGTGGGATTGGAAT+TGG 0.413486 2_4:+19192890 None:intergenic
TCGGGGTGGTGGTGGTTGAA+GGG 0.422426 2_4:+19192802 None:intergenic
TGAAGGGGTGGGGATAATGG+TGG 0.425891 2_4:+19192818 None:intergenic
GAAATTAAGAACTTTGTTGT+TGG 0.434538 2_4:-19190804 Msa0319460:CDS
TATGTCAGAAGATTTATTGA+TGG 0.435423 2_4:-19190628 Msa0319460:CDS
ACTGACACTAGGATATTGAT+TGG 0.442304 2_4:+19192458 None:intergenic
GATGTGGGTCGGGGTGGTGG+TGG 0.445392 2_4:+19192794 None:intergenic
TACGGGTGCTAGCGGGGTTA+GGG 0.449094 2_4:+19192865 None:intergenic
TGGAGTTAGAGTTTCAGAAT+TGG 0.449537 2_4:+19193070 None:intergenic
TGTGTATGTTGTGGACTAGA+TGG 0.450832 2_4:+19192312 None:intergenic
AAAGGTGGAATTTCGATTTG+AGG 0.455769 2_4:+19192743 None:intergenic
GACTTTAACCACCTCAATAG+TGG 0.457259 2_4:-19190606 Msa0319460:CDS
GGAATGTGAATAGCGGTGGT+GGG 0.459869 2_4:+19192636 None:intergenic
AGGGGTGGGGATAATGGTGG+TGG 0.468517 2_4:+19192821 None:intergenic
ACCTGAGAATTGCTAGTCTC+TGG 0.471222 2_4:+19190744 None:intergenic
GAGCATAACATCTTCTACTT+CGG 0.472162 2_4:+19192668 None:intergenic
GTTAATATTATTAGTGATGT+GGG 0.476825 2_4:+19192779 None:intergenic
GCCAGAGACTAGCAATTCTC+AGG 0.477878 2_4:-19190745 Msa0319460:CDS
TGTCCTTGGTGTGTTATATG+AGG 0.480802 2_4:-19192044 Msa0319460:CDS
TGAAGTCATTTGGTTTAAGC+GGG 0.482952 2_4:+19192934 None:intergenic
AGCGGTTGTGATTTCAATGC+AGG 0.486927 2_4:+19192359 None:intergenic
GTTAGCAAAGGTGGTGGTAG+TGG 0.488522 2_4:+19192569 None:intergenic
TTTGGAATACGGGTGCTAGC+GGG 0.488713 2_4:+19192858 None:intergenic
ATATTATTAGTGATGTGGGT+CGG 0.491960 2_4:+19192783 None:intergenic
ATGAAGTCATTTGGTTTAAG+CGG 0.492100 2_4:+19192933 None:intergenic
TTGCTTCTTTGTGTATGTTG+TGG 0.506190 2_4:+19192303 None:intergenic
AGAAACCCAAACTAGTTCTA+GGG 0.507577 2_4:-19192265 Msa0319460:CDS
AAAGAATGCCAAAATGTCCT+TGG 0.516011 2_4:-19192058 Msa0319460:CDS
ATTATTAGTGATGTGGGTCG+GGG 0.521424 2_4:+19192785 None:intergenic
GAATGTGAATAGCGGTGGTG+GGG 0.524266 2_4:+19192637 None:intergenic
GGTAGGAGTAGTGGCAGTCA+AGG 0.527115 2_4:+19192527 None:intergenic
CAAGGTGCTAATAGTGTTGG+CGG 0.530004 2_4:+19192545 None:intergenic
AGTTAGAGTTTCAGAATTGG+TGG 0.535695 2_4:+19193073 None:intergenic
GGAATAAAGCACCACTATTG+AGG 0.537769 2_4:+19190595 None:intergenic
TGTTAATATTATTAGTGATG+TGG 0.542659 2_4:+19192778 None:intergenic
GTTGGCGGGGTTAGCAAAGG+TGG 0.544813 2_4:+19192560 None:intergenic
GGTGGTGAGAGTAGTGATGG+TGG 0.545701 2_4:+19192614 None:intergenic
GATAGTGTTGGCAGTAGAGA+TGG 0.547801 2_4:+19192506 None:intergenic
GTAATTGGGGTAATGATAGT+GGG 0.548235 2_4:+19192591 None:intergenic
GATGTTACTGATGGTGGTGG+TGG 0.548240 2_4:+19193034 None:intergenic
AAGGTGCTAATAGTGTTGGC+GGG 0.550983 2_4:+19192546 None:intergenic
GTGGTGGTTAGGATTTGAGG+TGG 0.552967 2_4:+19193050 None:intergenic
AGTAGAGATGGTAGGAGTAG+TGG 0.558152 2_4:+19192518 None:intergenic
TGTTGTTGTTCAATGACTGT+TGG 0.564245 2_4:+19192711 None:intergenic
GGGTTAGGGATAATTGTGGT+GGG 0.569034 2_4:+19192879 None:intergenic
GGATATGATGTTACTGATGG+TGG 0.569473 2_4:+19193028 None:intergenic
TATGTTGTGGACTAGATGGA+TGG 0.570891 2_4:+19192316 None:intergenic
TGAACTTTCAACAGACTCAT+GGG 0.572196 2_4:-19192016 Msa0319460:CDS
TTAAAGGGTCTCCACATCCA+AGG 0.575832 2_4:-19191966 Msa0319460:intron
GTGGTGAGAGTAGTGATGGT+GGG 0.578827 2_4:+19192615 None:intergenic
ATGTATGAAAACCTTGGATG+TGG 0.583670 2_4:+19191955 None:intergenic
AGTCAAGGTGCTAATAGTGT+TGG 0.585343 2_4:+19192542 None:intergenic
ATGAACTTTCAACAGACTCA+TGG 0.586902 2_4:-19192017 Msa0319460:CDS
GTGTTGGCAGTAGAGATGGT+AGG 0.592781 2_4:+19192510 None:intergenic
GGCGGGGTTAGCAAAGGTGG+TGG 0.593505 2_4:+19192563 None:intergenic
ATTAGTGATGTGGGTCGGGG+TGG 0.595677 2_4:+19192788 None:intergenic
TTGGAATACGGGTGCTAGCG+GGG 0.599630 2_4:+19192859 None:intergenic
GGGGTTAGGGATAATTGTGG+TGG 0.606726 2_4:+19192878 None:intergenic
GGTGGGAATGTGAATAGCGG+TGG 0.610840 2_4:+19192632 None:intergenic
TCATGGGCATGGAGTAAACA+TGG 0.611520 2_4:-19192000 Msa0319460:CDS
AGTGTTCGTCATGATAGTGT+TGG 0.615034 2_4:+19192494 None:intergenic
AATTGGGGTAATGATAGTGG+GGG 0.618091 2_4:+19192593 None:intergenic
TAATTGGGGTAATGATAGTG+GGG 0.618793 2_4:+19192592 None:intergenic
TTTCAACAGACTCATGGGCA+TGG 0.620196 2_4:-19192011 Msa0319460:CDS
ATCATCATAGCATGATGATG+TGG 0.621279 2_4:+19192995 None:intergenic
AGTGTTGGCGGGGTTAGCAA+AGG 0.621663 2_4:+19192557 None:intergenic
GGTGGTGGTAGTGGTAATTG+GGG 0.628613 2_4:+19192578 None:intergenic
TGATGATGTGGTACTTTGAG+AGG 0.630706 2_4:+19193007 None:intergenic
TATGATGTTACTGATGGTGG+TGG 0.635627 2_4:+19193031 None:intergenic
ATTGGTGGCAGAAGTAGTCA+AGG 0.638558 2_4:+19193088 None:intergenic
AGAGGATATGATGTTACTGA+TGG 0.644683 2_4:+19193025 None:intergenic
TTCCCTCATATAACACACCA+AGG 0.662868 2_4:+19192041 None:intergenic
GTCCTTGGTGTGTTATATGA+GGG 0.664590 2_4:-19192043 Msa0319460:CDS
ATAAAGCACCACTATTGAGG+TGG 0.665488 2_4:+19190598 None:intergenic
GAAGTCATTTGGTTTAAGCG+GGG 0.666201 2_4:+19192935 None:intergenic
AGCGGGGTTAGGGATAATTG+TGG 0.672234 2_4:+19192875 None:intergenic
AGGTGCTAATAGTGTTGGCG+GGG 0.672241 2_4:+19192547 None:intergenic
GATGGTGGGAATGTGAATAG+CGG 0.710352 2_4:+19192629 None:intergenic
GTATGCACGATCAAAATGAG+CGG 0.723045 2_4:+19192341 None:intergenic
GGTGGCAGAAGTAGTCAAGG+TGG 0.726974 2_4:+19193091 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAATAAAAAATATATAT+AGG - chr2_4:19192207-19192226 Msa0319460:intron 0.0%
!!! TTTATTTTATTTTTTGTCTT+TGG + chr2_4:19192197-19192216 None:intergenic 10.0%
!!! ATAGAAAAAGTAATTTTACA+GGG + chr2_4:19191956-19191975 None:intergenic 15.0%
!!! AATAGAAAAAGTAATTTTAC+AGG + chr2_4:19191957-19191976 None:intergenic 15.0%
!!! CAATTTCAATATTTTTTCTT+AGG + chr2_4:19192031-19192050 None:intergenic 15.0%
!!! ATTTTAATCCTTATTTTGAT+GGG - chr2_4:19192066-19192085 Msa0319460:intron 15.0%
!! TTAATATGAATAATATTTGC+AGG - chr2_4:19192125-19192144 Msa0319460:intron 15.0%
!! TAATATGAATAATATTTGCA+GGG - chr2_4:19192126-19192145 Msa0319460:intron 15.0%
!! GTTAATATTATTAGTGATGT+GGG + chr2_4:19190957-19190976 None:intergenic 20.0%
!! TGTTAATATTATTAGTGATG+TGG + chr2_4:19190958-19190977 None:intergenic 20.0%
!!! TTTTTTCTGTAAGAAAAGAA+AGG + chr2_4:19191658-19191677 None:intergenic 20.0%
!! ATGGACAAAAATTCATTAAA+GGG - chr2_4:19191752-19191771 Msa0319460:intron 20.0%
!!! CATTTTAATCCTTATTTTGA+TGG - chr2_4:19192065-19192084 Msa0319460:intron 20.0%
!! AAAGTGAAAGATATAAACTT+AGG + chr2_4:19192334-19192353 None:intergenic 20.0%
!! TGATAAACAAACAATTGATA+CGG - chr2_4:19192368-19192387 Msa0319460:CDS 20.0%
!!! TTTATATAAAGGTATGCAAT+CGG + chr2_4:19192483-19192502 None:intergenic 20.0%
!! TATTAGTGCTCTTTATATAA+AGG + chr2_4:19192494-19192513 None:intergenic 20.0%
!! AAGAGATTGCATTATTTTCT+TGG + chr2_4:19190775-19190794 None:intergenic 25.0%
!!! TGGTAATGATTTTTGGAATA+CGG + chr2_4:19190889-19190908 None:intergenic 25.0%
! GTAAGAAAAGAAAGGTAAAA+TGG + chr2_4:19191650-19191669 None:intergenic 25.0%
! CATGGACAAAAATTCATTAA+AGG - chr2_4:19191751-19191770 Msa0319460:intron 25.0%
!! TTTTGAATGTATGAAAACCT+TGG + chr2_4:19191787-19191806 None:intergenic 25.0%
!! AAATAGTGACAGTAAACTTT+TGG - chr2_4:19191878-19191897 Msa0319460:intron 25.0%
!!! AATTTTAGCCCATCAAAATA+AGG + chr2_4:19192077-19192096 None:intergenic 25.0%
! TTAATCATTCTTTGCAATCT+TGG + chr2_4:19192592-19192611 None:intergenic 25.0%
!! TGATTTGTATCTTTAACAGT+AGG - chr2_4:19192619-19192638 Msa0319460:CDS 25.0%
! TCTTTAACAGTAGGAAAATA+CGG - chr2_4:19192628-19192647 Msa0319460:CDS 25.0%
!! GAAATCTATGAATGATTTGT+AGG - chr2_4:19192783-19192802 Msa0319460:CDS 25.0%
!!! TTCTCTACATATTTTTTTGC+TGG + chr2_4:19192842-19192861 None:intergenic 25.0%
!!! TCTTAATTTCTTCTGGTTTT+TGG + chr2_4:19192919-19192938 None:intergenic 25.0%
! ACAAAGTTCTTAATTTCTTC+TGG + chr2_4:19192926-19192945 None:intergenic 25.0%
!! GAAATTAAGAACTTTGTTGT+TGG - chr2_4:19192929-19192948 Msa0319460:CDS 25.0%
!!! ACAAGAGATTTTTGATTCAT+CGG + chr2_4:19193020-19193039 None:intergenic 25.0%
!!! TGTTCTGAAACAGAATTTAT+TGG - chr2_4:19193037-19193056 Msa0319460:CDS 25.0%
! TATGTCAGAAGATTTATTGA+TGG - chr2_4:19193105-19193124 Msa0319460:CDS 25.0%
! ATGAAGTCATTTGGTTTAAG+CGG + chr2_4:19190803-19190822 None:intergenic 30.0%
TCTAACTCTATGAAGTCATT+TGG + chr2_4:19190812-19190831 None:intergenic 30.0%
!!! GGTAATGATTTTTGGAATAC+GGG + chr2_4:19190888-19190907 None:intergenic 30.0%
ATATTATTAGTGATGTGGGT+CGG + chr2_4:19190953-19190972 None:intergenic 30.0%
! TAAAACCCTAGAACTAGTTT+GGG + chr2_4:19191476-19191495 None:intergenic 30.0%
! ATTACCTTTTTCTTGATCTG+AGG + chr2_4:19191525-19191544 None:intergenic 30.0%
! GTTCGATGAGATTTAAGTTA+TGG + chr2_4:19191622-19191641 None:intergenic 30.0%
! CTATTTAAAACATGTGGTGT+GGG + chr2_4:19191864-19191883 None:intergenic 30.0%
! ACTATTTAAAACATGTGGTG+TGG + chr2_4:19191865-19191884 None:intergenic 30.0%
! CTGTCACTATTTAAAACATG+TGG + chr2_4:19191870-19191889 None:intergenic 30.0%
ATCCTTGCAATTTCAAAGAA+TGG - chr2_4:19191901-19191920 Msa0319460:intron 30.0%
!! TACCATTCTTTGAAATTGCA+AGG + chr2_4:19191906-19191925 None:intergenic 30.0%
TAAAAACCAAAAGCCAAAGA+TGG - chr2_4:19192164-19192183 Msa0319460:intron 30.0%
!!! AATAAACCATCTTTGGCTTT+TGG + chr2_4:19192173-19192192 None:intergenic 30.0%
! CTTTGGAAATAAACCATCTT+TGG + chr2_4:19192180-19192199 None:intergenic 30.0%
ACACATATATATGCCATGAT+GGG - chr2_4:19192237-19192256 Msa0319460:CDS 30.0%
!! ATATGTTTTTGATCCCATCA+TGG + chr2_4:19192253-19192272 None:intergenic 30.0%
TTGCTCAATGGAAAAAGATA+CGG + chr2_4:19192551-19192570 None:intergenic 30.0%
! CTTTTTCCATTGAGCAATTA+AGG - chr2_4:19192554-19192573 Msa0319460:CDS 30.0%
ATGATATTCTAACAACCTAC+AGG + chr2_4:19192663-19192682 None:intergenic 30.0%
GCTTATATTGTGACGTATAA+AGG - chr2_4:19192881-19192900 Msa0319460:CDS 30.0%
AGTTAGAGTTTCAGAATTGG+TGG + chr2_4:19190663-19190682 None:intergenic 35.0%
TGGAGTTAGAGTTTCAGAAT+TGG + chr2_4:19190666-19190685 None:intergenic 35.0%
AGAGGATATGATGTTACTGA+TGG + chr2_4:19190711-19190730 None:intergenic 35.0%
! ATCATCATAGCATGATGATG+TGG + chr2_4:19190741-19190760 None:intergenic 35.0%
! TGAAGTCATTTGGTTTAAGC+GGG + chr2_4:19190802-19190821 None:intergenic 35.0%
TATTATTAGTGATGTGGGTC+GGG + chr2_4:19190952-19190971 None:intergenic 35.0%
! AAAGGTGGAATTTCGATTTG+AGG + chr2_4:19190993-19191012 None:intergenic 35.0%
TGTTGGAAGATGAAAAAAGG+TGG + chr2_4:19191008-19191027 None:intergenic 35.0%
GACTGTTGGAAGATGAAAAA+AGG + chr2_4:19191011-19191030 None:intergenic 35.0%
! TGTTGTTGTTCAATGACTGT+TGG + chr2_4:19191025-19191044 None:intergenic 35.0%
GAGCATAACATCTTCTACTT+CGG + chr2_4:19191068-19191087 None:intergenic 35.0%
! TAATTGGGGTAATGATAGTG+GGG + chr2_4:19191144-19191163 None:intergenic 35.0%
GTAATTGGGGTAATGATAGT+GGG + chr2_4:19191145-19191164 None:intergenic 35.0%
ACTGACACTAGGATATTGAT+TGG + chr2_4:19191278-19191297 None:intergenic 35.0%
GTTGAAAAATCACTGACACT+AGG + chr2_4:19191289-19191308 None:intergenic 35.0%
TTGCTTCTTTGTGTATGTTG+TGG + chr2_4:19191433-19191452 None:intergenic 35.0%
!! CTAGTTTGGGTTTCTAGAAT+TGG + chr2_4:19191463-19191482 None:intergenic 35.0%
! TAGAAACCCAAACTAGTTCT+AGG - chr2_4:19191467-19191486 Msa0319460:intron 35.0%
!! AGAAACCCAAACTAGTTCTA+GGG - chr2_4:19191468-19191487 Msa0319460:intron 35.0%
GTAAAACCCTAGAACTAGTT+TGG + chr2_4:19191477-19191496 None:intergenic 35.0%
!! GATTGTTGCTGATTTGTGTT+TGG + chr2_4:19191499-19191518 None:intergenic 35.0%
CAAACCTCAGATCAAGAAAA+AGG - chr2_4:19191518-19191537 Msa0319460:intron 35.0%
AAAGAATGCCAAAATGTCCT+TGG - chr2_4:19191675-19191694 Msa0319460:intron 35.0%
! ATAACACACCAAGGACATTT+TGG + chr2_4:19191686-19191705 None:intergenic 35.0%
ATGAACTTTCAACAGACTCA+TGG - chr2_4:19191716-19191735 Msa0319460:intron 35.0%
TGAACTTTCAACAGACTCAT+GGG - chr2_4:19191717-19191736 Msa0319460:intron 35.0%
ATGTATGAAAACCTTGGATG+TGG + chr2_4:19191781-19191800 None:intergenic 35.0%
CATTGCAAATATTCCACCTT+TGG - chr2_4:19191977-19191996 Msa0319460:CDS 35.0%
AGGAACTATAACAAACACCA+GGG + chr2_4:19192011-19192030 None:intergenic 35.0%
TAGGAACTATAACAAACACC+AGG + chr2_4:19192012-19192031 None:intergenic 35.0%
CACACATATATATGCCATGA+TGG - chr2_4:19192236-19192255 Msa0319460:CDS 35.0%
AAGAAATATGCCACTCATTG+TGG - chr2_4:19192450-19192469 Msa0319460:CDS 35.0%
AGAAATATGCCACTCATTGT+GGG - chr2_4:19192451-19192470 Msa0319460:CDS 35.0%
AATGGAAAAAGATACGGTGT+GGG + chr2_4:19192545-19192564 None:intergenic 35.0%
TCTTTGCAATCTTGGAAAGT+GGG + chr2_4:19192584-19192603 None:intergenic 35.0%
TTCTTTGCAATCTTGGAAAG+TGG + chr2_4:19192585-19192604 None:intergenic 35.0%
!! CAGCATCATTTTGTTATGTG+AGG + chr2_4:19192957-19192976 None:intergenic 35.0%
TCTGACATAGCTGTTATGTT+TGG + chr2_4:19193094-19193113 None:intergenic 35.0%
!! TATGATGTTACTGATGGTGG+TGG + chr2_4:19190705-19190724 None:intergenic 40.0%
GGATATGATGTTACTGATGG+TGG + chr2_4:19190708-19190727 None:intergenic 40.0%
! TGATGATGTGGTACTTTGAG+AGG + chr2_4:19190729-19190748 None:intergenic 40.0%
! GAAGTCATTTGGTTTAAGCG+GGG + chr2_4:19190801-19190820 None:intergenic 40.0%
! AATTGTGGTGGGATTGGAAT+TGG + chr2_4:19190846-19190865 None:intergenic 40.0%
!!! GTGGTGGTGGTAATGATTTT+TGG + chr2_4:19190896-19190915 None:intergenic 40.0%
ATTATTAGTGATGTGGGTCG+GGG + chr2_4:19190951-19190970 None:intergenic 40.0%
!! AATTGGGGTAATGATAGTGG+GGG + chr2_4:19191143-19191162 None:intergenic 40.0%
GGTAATTGGGGTAATGATAG+TGG + chr2_4:19191146-19191165 None:intergenic 40.0%
! AGTCAAGGTGCTAATAGTGT+TGG + chr2_4:19191194-19191213 None:intergenic 40.0%
! AGTGTTCGTCATGATAGTGT+TGG + chr2_4:19191242-19191261 None:intergenic 40.0%
GTATGCACGATCAAAATGAG+CGG + chr2_4:19191395-19191414 None:intergenic 40.0%
!! TATGTTGTGGACTAGATGGA+TGG + chr2_4:19191420-19191439 None:intergenic 40.0%
!! TGTGTATGTTGTGGACTAGA+TGG + chr2_4:19191424-19191443 None:intergenic 40.0%
! TGTCCTTGGTGTGTTATATG+AGG - chr2_4:19191689-19191708 Msa0319460:intron 40.0%
! GTCCTTGGTGTGTTATATGA+GGG - chr2_4:19191690-19191709 Msa0319460:intron 40.0%
TTCCCTCATATAACACACCA+AGG + chr2_4:19191695-19191714 None:intergenic 40.0%
!!! TGGGGAAAGTTGTTGGTTTT+TGG + chr2_4:19192526-19192545 None:intergenic 40.0%
ATGGAAAAAGATACGGTGTG+GGG + chr2_4:19192544-19192563 None:intergenic 40.0%
CAATGGAAAAAGATACGGTG+TGG + chr2_4:19192546-19192565 None:intergenic 40.0%
TTCCATTGAGCAATTAAGGC+TGG - chr2_4:19192558-19192577 Msa0319460:CDS 40.0%
AACAGTAGGAAAATACGGTG+TGG - chr2_4:19192633-19192652 Msa0319460:CDS 40.0%
GACTTTAACCACCTCAATAG+TGG - chr2_4:19193127-19193146 Msa0319460:CDS 40.0%
! ATAAAGCACCACTATTGAGG+TGG + chr2_4:19193138-19193157 None:intergenic 40.0%
! GGAATAAAGCACCACTATTG+AGG + chr2_4:19193141-19193160 None:intergenic 40.0%
! ATTGGTGGCAGAAGTAGTCA+AGG + chr2_4:19190648-19190667 None:intergenic 45.0%
! AGGGATAATTGTGGTGGGAT+TGG + chr2_4:19190852-19190871 None:intergenic 45.0%
GGGTTAGGGATAATTGTGGT+GGG + chr2_4:19190857-19190876 None:intergenic 45.0%
!!! TTTTGGAATACGGGTGCTAG+CGG + chr2_4:19190879-19190898 None:intergenic 45.0%
!! GATGGTGGGAATGTGAATAG+CGG + chr2_4:19191107-19191126 None:intergenic 45.0%
!! AGGTGGTGGTAGTGGTAATT+GGG + chr2_4:19191159-19191178 None:intergenic 45.0%
!! AAGGTGGTGGTAGTGGTAAT+TGG + chr2_4:19191160-19191179 None:intergenic 45.0%
!! AAGGTGCTAATAGTGTTGGC+GGG + chr2_4:19191190-19191209 None:intergenic 45.0%
! CAAGGTGCTAATAGTGTTGG+CGG + chr2_4:19191191-19191210 None:intergenic 45.0%
AGTAGAGATGGTAGGAGTAG+TGG + chr2_4:19191218-19191237 None:intergenic 45.0%
!! GATAGTGTTGGCAGTAGAGA+TGG + chr2_4:19191230-19191249 None:intergenic 45.0%
AGCGGTTGTGATTTCAATGC+AGG + chr2_4:19191377-19191396 None:intergenic 45.0%
TTTCAACAGACTCATGGGCA+TGG - chr2_4:19191722-19191741 Msa0319460:intron 45.0%
TCATGGGCATGGAGTAAACA+TGG - chr2_4:19191733-19191752 Msa0319460:intron 45.0%
TTAAAGGGTCTCCACATCCA+AGG - chr2_4:19191767-19191786 Msa0319460:intron 45.0%
CAGGGACAAAAAACCAAAGG+TGG + chr2_4:19191993-19192012 None:intergenic 45.0%
!!! CACCTTTGGTTTTTTGTCCC+TGG - chr2_4:19191991-19192010 Msa0319460:CDS 45.0%
CACCAGGGACAAAAAACCAA+AGG + chr2_4:19191996-19192015 None:intergenic 45.0%
ATATGCCACTCATTGTGGGA+CGG - chr2_4:19192455-19192474 Msa0319460:CDS 45.0%
! ACCTGAGAATTGCTAGTCTC+TGG + chr2_4:19192992-19193011 None:intergenic 45.0%
!! AATATTATTCATATTAAAAT+TGG + chr2_4:19192122-19192141 None:intergenic 5.0%
GTGGTGGTTAGGATTTGAGG+TGG + chr2_4:19190686-19190705 None:intergenic 50.0%
!! GTGGTGGTGGTTAGGATTTG+AGG + chr2_4:19190689-19190708 None:intergenic 50.0%
!! TACTGATGGTGGTGGTGGTT+AGG + chr2_4:19190697-19190716 None:intergenic 50.0%
!! GATGTTACTGATGGTGGTGG+TGG + chr2_4:19190702-19190721 None:intergenic 50.0%
GGGGTTAGGGATAATTGTGG+TGG + chr2_4:19190858-19190877 None:intergenic 50.0%
AGCGGGGTTAGGGATAATTG+TGG + chr2_4:19190861-19190880 None:intergenic 50.0%
! TTTGGAATACGGGTGCTAGC+GGG + chr2_4:19190878-19190897 None:intergenic 50.0%
GAATGTGAATAGCGGTGGTG+GGG + chr2_4:19191099-19191118 None:intergenic 50.0%
GGAATGTGAATAGCGGTGGT+GGG + chr2_4:19191100-19191119 None:intergenic 50.0%
GTGGTGAGAGTAGTGATGGT+GGG + chr2_4:19191121-19191140 None:intergenic 50.0%
!! GGTGGTGGTAGTGGTAATTG+GGG + chr2_4:19191158-19191177 None:intergenic 50.0%
! GTTAGCAAAGGTGGTGGTAG+TGG + chr2_4:19191167-19191186 None:intergenic 50.0%
!! AGGTGCTAATAGTGTTGGCG+GGG + chr2_4:19191189-19191208 None:intergenic 50.0%
! GTGTTGGCAGTAGAGATGGT+AGG + chr2_4:19191226-19191245 None:intergenic 50.0%
GTGGTGTGGGTGTGTGTATT+AGG + chr2_4:19191851-19191870 None:intergenic 50.0%
TACGGTGTGGGGAAAGTTGT+TGG + chr2_4:19192533-19192552 None:intergenic 50.0%
GGCCAGCCTTAATTGCTCAA+TGG + chr2_4:19192563-19192582 None:intergenic 50.0%
ATACGGTGTGGACAACCTGT+AGG - chr2_4:19192645-19192664 Msa0319460:CDS 50.0%
GCCAGAGACTAGCAATTCTC+AGG - chr2_4:19192988-19193007 Msa0319460:CDS 50.0%
GGTGGCAGAAGTAGTCAAGG+TGG + chr2_4:19190645-19190664 None:intergenic 55.0%
TTGGAATACGGGTGCTAGCG+GGG + chr2_4:19190877-19190896 None:intergenic 55.0%
! TGAAGGGGTGGGGATAATGG+TGG + chr2_4:19190918-19190937 None:intergenic 55.0%
GGTTGAAGGGGTGGGGATAA+TGG + chr2_4:19190921-19190940 None:intergenic 55.0%
ATTAGTGATGTGGGTCGGGG+TGG + chr2_4:19190948-19190967 None:intergenic 55.0%
GGGAATGTGAATAGCGGTGG+TGG + chr2_4:19191101-19191120 None:intergenic 55.0%
GGTGGGAATGTGAATAGCGG+TGG + chr2_4:19191104-19191123 None:intergenic 55.0%
GGTGGTGAGAGTAGTGATGG+TGG + chr2_4:19191122-19191141 None:intergenic 55.0%
!! TGGGGTAATGATAGTGGGGG+TGG + chr2_4:19191140-19191159 None:intergenic 55.0%
!! AGTGTTGGCGGGGTTAGCAA+AGG + chr2_4:19191179-19191198 None:intergenic 55.0%
GGTAGGAGTAGTGGCAGTCA+AGG + chr2_4:19191209-19191228 None:intergenic 55.0%
GTAATGTGTGCGCGCGCAAA+TGG - chr2_4:19192716-19192735 Msa0319460:CDS 55.0%
TACGGGTGCTAGCGGGGTTA+GGG + chr2_4:19190871-19190890 None:intergenic 60.0%
ATACGGGTGCTAGCGGGGTT+AGG + chr2_4:19190872-19190891 None:intergenic 60.0%
!! GGGGATAATGGTGGTGGTGG+TGG + chr2_4:19190909-19190928 None:intergenic 60.0%
!! GGTGGGGATAATGGTGGTGG+TGG + chr2_4:19190912-19190931 None:intergenic 60.0%
! AGGGGTGGGGATAATGGTGG+TGG + chr2_4:19190915-19190934 None:intergenic 60.0%
!! TGGTGGTGGTTGAAGGGGTG+GGG + chr2_4:19190928-19190947 None:intergenic 60.0%
!! GTGGTGGTGGTTGAAGGGGT+GGG + chr2_4:19190929-19190948 None:intergenic 60.0%
!! TCGGGGTGGTGGTGGTTGAA+GGG + chr2_4:19190934-19190953 None:intergenic 60.0%
GGGGGTGGTGAGAGTAGTGA+TGG + chr2_4:19191125-19191144 None:intergenic 60.0%
! GTTGGCGGGGTTAGCAAAGG+TGG + chr2_4:19191176-19191195 None:intergenic 60.0%
CGGTTCCGTCCCACAATGAG+TGG + chr2_4:19192463-19192482 None:intergenic 60.0%
!! GGTGGTGGTGGTTGAAGGGG+TGG + chr2_4:19190930-19190949 None:intergenic 65.0%
!! CGGGGTGGTGGTGGTTGAAG+GGG + chr2_4:19190933-19190952 None:intergenic 65.0%
!! GTCGGGGTGGTGGTGGTTGA+AGG + chr2_4:19190935-19190954 None:intergenic 65.0%
AGTGATGTGGGTCGGGGTGG+TGG + chr2_4:19190945-19190964 None:intergenic 65.0%
! GGCGGGGTTAGCAAAGGTGG+TGG + chr2_4:19191173-19191192 None:intergenic 65.0%
! GATGTGGGTCGGGGTGGTGG+TGG + chr2_4:19190942-19190961 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 19190590 19193165 19190590 ID=Msa0319460;Name=Msa0319460
chr2_4 mRNA 19190590 19193165 19190590 ID=Msa0319460-mRNA-1;Parent=Msa0319460;Name=Msa0319460-mRNA-1;_AED=0.06;_eAED=0.06;_QI=0|0|0|1|1|1|3|0|475
chr2_4 exon 19192216 19193165 19192216 ID=Msa0319460-mRNA-1:exon:7120;Parent=Msa0319460-mRNA-1
chr2_4 exon 19191967 19192083 19191967 ID=Msa0319460-mRNA-1:exon:7119;Parent=Msa0319460-mRNA-1
chr2_4 exon 19190590 19190950 19190590 ID=Msa0319460-mRNA-1:exon:7118;Parent=Msa0319460-mRNA-1
chr2_4 CDS 19192216 19193165 19192216 ID=Msa0319460-mRNA-1:cds;Parent=Msa0319460-mRNA-1
chr2_4 CDS 19191967 19192083 19191967 ID=Msa0319460-mRNA-1:cds;Parent=Msa0319460-mRNA-1
chr2_4 CDS 19190590 19190950 19190590 ID=Msa0319460-mRNA-1:cds;Parent=Msa0319460-mRNA-1
Gene Sequence

>Msa0319460

ATGGATAATGATGATTGGGATCTCTTTTCTATTGTGCGTAGCTGCAACACTACCACCTTGACTACTTCTGCCACCAATTCTGAAACTCTAACTCCACCTCAAATCCTAACCACCACCACCATCAGTAACATCATATCCTCTCAAAGTACCACATCATCATGCTATGATGATTTTAGTTTTATCCAAGAAAATAATGCAATCTCTTTTGCCCCGCTTAAACCAAATGACTTCATAGAGTTAGATAAATTGATGACCAATTCCAATCCCACCACAATTATCCCTAACCCCGCTAGCACCCGTATTCCAAAAATCATTACCACCACCACCACCATTATCCCCACCCCTTCAACCACCACCACCCCGACCCACATCACTAATAATATTAACACTAGTGTTCATGCCTCAAATCGAAATTCCACCTTTTTTCATCTTCCAACAGTCATTGAACAACAACAAATGCAACGAAATGAGTTCACCGAAGTAGAAGATGTTATGCTCAAATTCAACCCCACCACCGCTATTCACATTCCCACCATCACTACTCTCACCACCCCCACTATCATTACCCCAATTACCACTACCACCACCTTTGCTAACCCCGCCAACACTATTAGCACCTTGACTGCCACTACTCCTACCATCTCTACTGCCAACACTATCATGACGAACACTAGCGTTCATGAAACCAATCAATATCCTAGTGTCAGTGATTTTTCAACATTCGTTGAACAACAACATATTCATCAAAGCAACAACAATCAAGTGTTTGTTTCGAAACATGTTGCCTGCATTGAAATCACAACCGCTCATTTTGATCGTGCATACAACCATCCATCTAGTCCACAACATACACAAAGAAGCAATCAACTTCCAATTCTAGAAACCCAAACTAGTTCTAGGGTTTTACCAAACACAAATCAGCAACAATCAAACCTCAGATCAAGAAAAAGAAAAAAGAATGCCAAAATGTCCTTGGTGTGTTATATGAGGGAAGATGAACTTTCAACAGACTCATGGGCATGGAGTAAACATGGACAAAAATTCATTAAAGGGTCTCCACATCCAAGGTGCTATTATAAGTGCAGCAGTTTCGCAAATTGTCCAGCAAAAAAATATGTAGAGAAAAGCAAAACTCATGAGAACGCTTATATTGTGACGTATAAAGGAGAGCATGATCACCAAAAACCAGAAGAAATTAAGAACTTTGTTGTTGGTACCTCACATAACAAAATGATGCTGAAGTTTGATGAGCCAGAGACTAGCAATTCTCAGGTTTTTTCCGATGAATCAAAAATCTCTTGTTCTGAAACAGAATTTATTGGAAACTCTAATGATGATGATGACATTTTGATACCAAACATAACAGCTATGTCAGAAGATTTATTGATGGACTTTAACCACCTCAATAGTGGTGCTTTATTCCCATAG
Protein sequence

>Msa0319460

MDNDDWDLFSIVRSCNTTTLTTSATNSETLTPPQILTTTTISNIISSQSTTSSCYDDFSFIQENNAISFAPLKPNDFIELDKLMTNSNPTTIIPNPASTRIPKIITTTTTIIPTPSTTTTPTHITNNINTSVHASNRNSTFFHLPTVIEQQQMQRNEFTEVEDVMLKFNPTTAIHIPTITTLTTPTIITPITTTTTFANPANTISTLTATTPTISTANTIMTNTSVHETNQYPSVSDFSTFVEQQHIHQSNNNQVFVSKHVACIEITTAHFDRAYNHPSSPQHTQRSNQLPILETQTSSRVLPNTNQQQSNLRSRKRKKNAKMSLVCYMREDELSTDSWAWSKHGQKFIKGSPHPRCYYKCSSFANCPAKKYVEKSKTHENAYIVTYKGEHDHQKPEEIKNFVVGTSHNKMMLKFDEPETSNSQVFSDESKISCSETEFIGNSNDDDDILIPNITAMSEDLLMDFNHLNSGALFP*