AlfalfaGEDB | Msa0321030

M. sativa cultivar zhongmu-4 / Msa0321030

KEGG: K09260
GO: GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0010154 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0022414 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0042995 GO:0043076 GO:0043078 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0061458 GO:0065007 GO:0080090 GO:0090406 GO:0097159 GO:0120025 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00319.21

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0321030	XP_003594864.1	91.139	158	14	0	1	158	1	158	2.26e-89	267

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0321030	sp\|Q9FJK3\|AGL80_ARATH	41.667	156	87	2	1	154	1	154	6.97e-31	116

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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TFs/TRs:

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Gene ID	Type	Classification
Msa0321030	TF	MADS-M-type

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0321030	MtrunA17_Chr2g0296861	91.139	158	14	0	1	158	1	158	2.08e-93	267
Msa0321030	MtrunA17_Chr3g0107381	46.203	158	85	0	1	158	1	158	2.13e-46	150
Msa0321030	MtrunA17_Chr3g0107461	49.351	154	77	1	1	153	1	154	4.64e-46	147
Msa0321030	MtrunA17_Chr3g0110131	44.156	154	86	0	1	154	1	154	4.13e-38	130
Msa0321030	MtrunA17_Chr4g0014321	37.662	154	95	1	1	153	1	154	6.75e-35	121
Msa0321030	MtrunA17_Chr3g0105791	47.541	122	63	1	30	150	2	123	1.07e-33	115
Msa0321030	MtrunA17_Chr4g0031421	46.400	125	66	1	30	153	2	126	3.21e-33	114
Msa0321030	MtrunA17_Chr2g0283941	38.750	160	93	2	1	157	1	158	5.38e-33	117
Msa0321030	MtrunA17_Chr3g0107331	39.597	149	90	0	4	152	3	151	6.29e-33	114
Msa0321030	MtrunA17_Chr4g0013041	35.849	159	99	2	1	158	1	157	5.87e-32	113

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0321030	AT5G48670.1	41.667	156	87	2	1	154	1	154	7.08e-32	116
Msa0321030	AT3G05860.2	36.913	149	94	0	1	149	1	149	2.57e-29	107
Msa0321030	AT1G65300.1	37.086	151	95	0	4	154	3	153	3.13e-29	108
Msa0321030	AT1G65330.1	39.333	150	89	2	6	154	5	153	3.55e-29	108
Msa0321030	AT3G05860.3	39.103	156	81	3	1	149	1	149	1.37e-28	106
Msa0321030	AT3G05860.1	39.103	156	81	3	1	149	1	149	1.52e-28	106
Msa0321030	AT1G31630.1	33.333	153	91	3	6	153	5	151	7.02e-24	95.5
Msa0321030	AT1G31640.1	35.915	142	90	1	11	152	10	150	2.05e-23	95.5
Msa0321030	AT5G27810.1	43.182	88	50	0	30	117	2	89	7.67e-23	87.8
Msa0321030	AT5G26650.1	32.237	152	103	0	4	155	2	153	1.74e-22	92.0

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Find 27 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGGAAAGCTGGATCATG+AGG	0.532487	2_4:-22028061	Msa0321030:CDS
AAAGGAAAGCTGGATCATGA+GGG	0.661580	2_4:-22028060	Msa0321030:CDS
AAGAAAATGAATGATCTACT+TGG	0.551967	2_4:-22027970	Msa0321030:CDS
ACATAACTGATGACTTGAAG+AGG	0.573872	2_4:-22028657	Msa0321030:CDS
ACATTCAACAAAAGGAAGCA+TGG	0.630577	2_4:-22028628	Msa0321030:intron
ACCGAAAAGAAGTCGATGAG+AGG	0.615793	2_4:-22027867	Msa0321030:CDS
ACGGTTTATCCATCAAAAGA+AGG	0.566324	2_4:-22028135	Msa0321030:CDS
ACTTGGTGAATTCATTCATA+CGG	0.496749	2_4:-22027953	Msa0321030:CDS
AGAAGGAGTCCAGATGGTGT+TGG	0.590630	2_4:-22028118	Msa0321030:CDS
AGAGAAGTTGAAGAAACAAA+AGG	0.500916	2_4:-22028007	Msa0321030:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CTTCTCTTTCGATTTCATTA+TGG	+	chr2_4:22028511-22028530	None:intergenic	30.0%
AGAGAAGTTGAAGAAACAAA+AGG	-	chr2_4:22028524-22028543	Msa0321030:intron	30.0%
GAAGATCTATCATTGCTTAT+TGG	-	chr2_4:22028636-22028655	Msa0321030:CDS	30.0%
CTTCTCTTTCGATTTCATTA+TGG	+	chr2_4:22028511-22028530	None:intergenic	30.0%
AGAGAAGTTGAAGAAACAAA+AGG	-	chr2_4:22028524-22028543	Msa0321030:intron	30.0%
GAAGATCTATCATTGCTTAT+TGG	-	chr2_4:22028636-22028655	Msa0321030:CDS	30.0%
ACATAACTGATGACTTGAAG+AGG	-	chr2_4:22027874-22027893	Msa0321030:CDS	35.0%
GGAAGACAACATTCAACAAA+AGG	-	chr2_4:22027895-22027914	Msa0321030:CDS	35.0%
ACATTCAACAAAAGGAAGCA+TGG	-	chr2_4:22027903-22027922	Msa0321030:CDS	35.0%
AAGTTGACTGCATAACCATT+AGG	-	chr2_4:22028052-22028071	Msa0321030:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_4	gene	22027846	22028707	22027846	ID=Msa0321030;Name=Msa0321030
chr2_4	mRNA	22027846	22028707	22027846	ID=Msa0321030-mRNA-1;Parent=Msa0321030;Name=Msa0321030-mRNA-1;_AED=0.29;_eAED=0.29;_QI=0\|0\|0\|1\|1\|1\|2\|0\|158
chr2_4	exon	22028629	22028707	22028629	ID=Msa0321030-mRNA-1:exon:8084;Parent=Msa0321030-mRNA-1
chr2_4	exon	22027846	22028243	22027846	ID=Msa0321030-mRNA-1:exon:8083;Parent=Msa0321030-mRNA-1
chr2_4	CDS	22028629	22028707	22028629	ID=Msa0321030-mRNA-1:cds;Parent=Msa0321030-mRNA-1
chr2_4	CDS	22027846	22028243	22027846	ID=Msa0321030-mRNA-1:cds;Parent=Msa0321030-mRNA-1

Gene Sequence

>Msa0321030

ATGGCTGATAAAAATATGAGAGTTGACTACATAACTGATGACTTGAAGAGGAAGACAACATTCAACAAAAGGAAGCATGGTATAACTAAGAAGATTAGTGAAATCACCACACTTTGTGGAATTGATGCATGTGGTATAATCTATGATGAAAACAATGATGGAGCAACGGTTTATCCATCAAAAGAAGGAGTCCAGATGGTGTTGGACAAGTTTAGGAGCTTTCCTGAATCGGAACAAAGAAAAGGAAAGCTGGATCATGAGGGCTTTTTGAGACAATCCATAATGAAATCGAAAGAGAAGTTGAAGAAACAAAAGGACAGGTCCAAGAAGAAGAAAATGAATGATCTACTTGGTGAATTCATTCATACGGGTGAATTTGATGGAAATGTGAGCGAGAATGATCTCGAAGATCTATCATTGCTTATTGGTGATTACCGAAAAGAAGTCGATGAGAGGATCGAATCAAGTCAAACCTGA

Protein sequence

>Msa0321030

MADKNMRVDYITDDLKRKTTFNKRKHGITKKISEITTLCGIDACGIIYDENNDGATVYPSKEGVQMVLDKFRSFPESEQRKGKLDHEGFLRQSIMKSKEKLKKQKDRSKKKKMNDLLGEFIHTGEFDGNVSENDLEDLSLLIGDYRKEVDERIESSQT*