AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0321030


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0321030 MtrunA17_Chr2g0296861 91.139 158 14 0 1 158 1 158 2.08e-93 267
Msa0321030 MtrunA17_Chr3g0107381 46.203 158 85 0 1 158 1 158 2.13e-46 150
Msa0321030 MtrunA17_Chr3g0107461 49.351 154 77 1 1 153 1 154 4.64e-46 147
Msa0321030 MtrunA17_Chr3g0110131 44.156 154 86 0 1 154 1 154 4.13e-38 130
Msa0321030 MtrunA17_Chr4g0014321 37.662 154 95 1 1 153 1 154 6.75e-35 121
Msa0321030 MtrunA17_Chr3g0105791 47.541 122 63 1 30 150 2 123 1.07e-33 115
Msa0321030 MtrunA17_Chr4g0031421 46.400 125 66 1 30 153 2 126 3.21e-33 114
Msa0321030 MtrunA17_Chr2g0283941 38.750 160 93 2 1 157 1 158 5.38e-33 117
Msa0321030 MtrunA17_Chr3g0107331 39.597 149 90 0 4 152 3 151 6.29e-33 114
Msa0321030 MtrunA17_Chr4g0013041 35.849 159 99 2 1 158 1 157 5.87e-32 113
Msa0321030 MtrunA17_Chr5g0432691 33.333 153 102 0 1 153 1 153 1.15e-30 108
Msa0321030 MtrunA17_Chr4g0014031 37.179 156 97 1 1 155 1 156 2.11e-29 107
Msa0321030 MtrunA17_Chr8g0351751 37.975 158 95 2 1 155 1 158 2.40e-29 107
Msa0321030 MtrunA17_Chr3g0107361 36.486 148 94 0 6 153 5 152 3.57e-29 104
Msa0321030 MtrunA17_Chr3g0106421 42.308 130 74 1 30 158 2 131 9.96e-29 105
Msa0321030 MtrunA17_Chr1g0197461 33.333 153 101 1 6 158 5 156 1.41e-28 103
Msa0321030 MtrunA17_Chr3g0107351 35.135 148 96 0 6 153 5 152 2.94e-28 102
Msa0321030 MtrunA17_Chr5g0420351 34.194 155 101 1 1 155 1 154 3.88e-28 102
Msa0321030 MtrunA17_Chr4g0014121 36.943 157 98 1 1 156 29 185 3.34e-27 100
Msa0321030 MtrunA17_Chr1g0188681 33.987 153 100 1 1 153 1 152 4.30e-27 99.4
Msa0321030 MtrunA17_Chr3g0107341 35.417 144 93 0 6 149 5 148 2.21e-26 97.8
Msa0321030 MtrunA17_Chr1g0197261 30.968 155 106 1 1 155 1 154 9.26e-24 90.9
Msa0321030 MtrunA17_Chr2g0296831 36.752 117 74 0 1 117 1 117 1.84e-23 89.0
Msa0321030 MtrunA17_Chr4g0014131 35.032 157 101 1 1 156 1 157 8.50e-23 90.1
Msa0321030 MtrunA17_Chr3g0106501 38.583 127 77 1 30 155 2 128 4.20e-22 86.7
Msa0321030 MtrunA17_Chr4g0012761 35.897 156 97 3 1 155 1 154 4.58e-22 86.7
Msa0321030 MtrunA17_Chr1g0197321 31.210 157 104 3 1 155 1 155 9.25e-22 85.9
Msa0321030 MtrunA17_Chr7g0218151 32.903 155 103 1 1 155 1 154 1.26e-21 85.5
Msa0321030 MtrunA17_Chr1g0193311 34.194 155 101 1 1 155 1 154 2.27e-21 84.7
Msa0321030 MtrunA17_Chr1g0188661 30.323 155 107 1 1 155 1 154 4.85e-20 81.3
Msa0321030 MtrunA17_Chr1g0188651 36.129 155 92 4 1 153 1 150 4.20e-18 76.3
Msa0321030 MtrunA17_Chr4g0008701 28.814 118 80 1 11 128 14 127 4.10e-11 60.1
Msa0321030 MtrunA17_Chr7g0267601 27.273 121 78 2 11 128 14 127 5.27e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0321030 AT5G48670.1 41.667 156 87 2 1 154 1 154 7.08e-32 116
Msa0321030 AT3G05860.2 36.913 149 94 0 1 149 1 149 2.57e-29 107
Msa0321030 AT1G65300.1 37.086 151 95 0 4 154 3 153 3.13e-29 108
Msa0321030 AT1G65330.1 39.333 150 89 2 6 154 5 153 3.55e-29 108
Msa0321030 AT3G05860.3 39.103 156 81 3 1 149 1 149 1.37e-28 106
Msa0321030 AT3G05860.1 39.103 156 81 3 1 149 1 149 1.52e-28 106
Msa0321030 AT1G31630.1 33.333 153 91 3 6 153 5 151 7.02e-24 95.5
Msa0321030 AT1G31640.1 35.915 142 90 1 11 152 10 150 2.05e-23 95.5
Msa0321030 AT5G27810.1 43.182 88 50 0 30 117 2 89 7.67e-23 87.8
Msa0321030 AT5G26650.1 32.237 152 103 0 4 155 2 153 1.74e-22 92.0
Msa0321030 AT5G27960.1 31.579 152 104 0 4 155 2 153 1.39e-21 89.0
Msa0321030 AT1G22590.2 36.111 108 69 0 11 118 11 118 5.23e-21 84.3
Msa0321030 AT2G28700.1 32.680 153 95 3 1 149 1 149 6.04e-20 84.7
Msa0321030 AT2G40210.1 32.479 117 79 0 1 117 1 117 9.62e-15 70.5
Msa0321030 AT5G26580.1 28.571 154 96 3 1 154 1 140 2.97e-14 69.3
Msa0321030 AT5G26630.1 26.623 154 113 0 1 154 1 154 3.35e-14 67.8
Msa0321030 AT5G06500.1 28.182 110 75 1 1 110 1 106 4.39e-13 65.1

Find 27 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGATCTATCATTGCTTAT+TGG 0.201999 2_4:-22027895 Msa0321030:CDS
CTTCTCTTTCGATTTCATTA+TGG 0.221424 2_4:+22028023 None:intergenic
ATTCATACGGGTGAATTTGA+TGG 0.333961 2_4:-22027940 Msa0321030:CDS
AGATGGTGTTGGACAAGTTT+AGG 0.350542 2_4:-22028107 Msa0321030:CDS
GTTTAGGAGCTTTCCTGAAT+CGG 0.417309 2_4:-22028091 Msa0321030:CDS
TAAACTTGTCCAACACCATC+TGG 0.418271 2_4:+22028109 None:intergenic
AGTGAAATCACCACACTTTG+TGG 0.451943 2_4:-22028204 Msa0321030:CDS
GGAAGACAACATTCAACAAA+AGG 0.464060 2_4:-22028636 Msa0321030:CDS
ATCTATGATGAAAACAATGA+TGG 0.489972 2_4:-22028162 Msa0321030:CDS
ACTTGGTGAATTCATTCATA+CGG 0.496749 2_4:-22027953 Msa0321030:CDS
AGAGAAGTTGAAGAAACAAA+AGG 0.500916 2_4:-22028007 Msa0321030:CDS
CCTGAATCGGAACAAAGAAA+AGG 0.516491 2_4:-22028078 Msa0321030:CDS
GGAACAAAGAAAAGGAAAGC+TGG 0.520124 2_4:-22028070 Msa0321030:CDS
AGTTGAAGAAACAAAAGGAC+AGG 0.532305 2_4:-22028002 Msa0321030:CDS
AAAAGGAAAGCTGGATCATG+AGG 0.532487 2_4:-22028061 Msa0321030:CDS
CTTGGTGAATTCATTCATAC+GGG 0.535817 2_4:-22027952 Msa0321030:CDS
AAGAAAATGAATGATCTACT+TGG 0.551967 2_4:-22027970 Msa0321030:CDS
ACGGTTTATCCATCAAAAGA+AGG 0.566324 2_4:-22028135 Msa0321030:CDS
ACATAACTGATGACTTGAAG+AGG 0.573872 2_4:-22028657 Msa0321030:CDS
AGAAGGAGTCCAGATGGTGT+TGG 0.590630 2_4:-22028118 Msa0321030:CDS
ACCGAAAAGAAGTCGATGAG+AGG 0.615793 2_4:-22027867 Msa0321030:CDS
ATCAAAAGAAGGAGTCCAGA+TGG 0.615817 2_4:-22028124 Msa0321030:CDS
TGCATCAATTCCACAAAGTG+TGG 0.616904 2_4:+22028194 None:intergenic
TGAAAACAATGATGGAGCAA+CGG 0.627126 2_4:-22028154 Msa0321030:CDS
ACATTCAACAAAAGGAAGCA+TGG 0.630577 2_4:-22028628 Msa0321030:intron
AAAGGAAAGCTGGATCATGA+GGG 0.661580 2_4:-22028060 Msa0321030:CDS
CTTTGTGGAATTGATGCATG+TGG 0.684212 2_4:-22028189 Msa0321030:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAAAATAACAATAATTTCAA+AGG + chr2_4:22028196-22028215 None:intergenic 15.0%
!! GAAAATAACAATAATTTCAA+AGG + chr2_4:22028196-22028215 None:intergenic 15.0%
!! AGTGTTCTTTATAGAAATAA+AGG + chr2_4:22027946-22027965 None:intergenic 20.0%
!!! TTTCATATTTTTAGTATCCA+TGG + chr2_4:22028034-22028053 None:intergenic 20.0%
!! TCATATTGATAAATTCCTAA+TGG + chr2_4:22028070-22028089 None:intergenic 20.0%
!! ACATAACTGATATGTTATTA+TGG + chr2_4:22028170-22028189 None:intergenic 20.0%
!!! ACAATATTTTAAGATCATGT+CGG - chr2_4:22028216-22028235 Msa0321030:CDS 20.0%
!! AGTGTTCTTTATAGAAATAA+AGG + chr2_4:22027946-22027965 None:intergenic 20.0%
!!! TTTCATATTTTTAGTATCCA+TGG + chr2_4:22028034-22028053 None:intergenic 20.0%
!! TCATATTGATAAATTCCTAA+TGG + chr2_4:22028070-22028089 None:intergenic 20.0%
!! ACATAACTGATATGTTATTA+TGG + chr2_4:22028170-22028189 None:intergenic 20.0%
!!! ACAATATTTTAAGATCATGT+CGG - chr2_4:22028216-22028235 Msa0321030:CDS 20.0%
!!! GTTTTTTTTTATCACTACCA+TGG - chr2_4:22028014-22028033 Msa0321030:CDS 25.0%
! ATCTATGATGAAAACAATGA+TGG - chr2_4:22028369-22028388 Msa0321030:intron 25.0%
!! ATCATTCATTTTCTTCTTCT+TGG + chr2_4:22028556-22028575 None:intergenic 25.0%
! AAGAAAATGAATGATCTACT+TGG - chr2_4:22028561-22028580 Msa0321030:intron 25.0%
!!! GTTTTTTTTTATCACTACCA+TGG - chr2_4:22028014-22028033 Msa0321030:CDS 25.0%
! ATCTATGATGAAAACAATGA+TGG - chr2_4:22028369-22028388 Msa0321030:intron 25.0%
!! ATCATTCATTTTCTTCTTCT+TGG + chr2_4:22028556-22028575 None:intergenic 25.0%
! AAGAAAATGAATGATCTACT+TGG - chr2_4:22028561-22028580 Msa0321030:intron 25.0%
!! GTTTTGTTGTGTTTTTCAAC+AGG - chr2_4:22028288-22028307 Msa0321030:intron 30.0%
CTTCTCTTTCGATTTCATTA+TGG + chr2_4:22028511-22028530 None:intergenic 30.0%
AGAGAAGTTGAAGAAACAAA+AGG - chr2_4:22028524-22028543 Msa0321030:intron 30.0%
! ACTTGGTGAATTCATTCATA+CGG - chr2_4:22028578-22028597 Msa0321030:intron 30.0%
GAAGATCTATCATTGCTTAT+TGG - chr2_4:22028636-22028655 Msa0321030:CDS 30.0%
!! GTTTTGTTGTGTTTTTCAAC+AGG - chr2_4:22028288-22028307 Msa0321030:intron 30.0%
CTTCTCTTTCGATTTCATTA+TGG + chr2_4:22028511-22028530 None:intergenic 30.0%
AGAGAAGTTGAAGAAACAAA+AGG - chr2_4:22028524-22028543 Msa0321030:intron 30.0%
! ACTTGGTGAATTCATTCATA+CGG - chr2_4:22028578-22028597 Msa0321030:intron 30.0%
GAAGATCTATCATTGCTTAT+TGG - chr2_4:22028636-22028655 Msa0321030:CDS 30.0%
ACATAACTGATGACTTGAAG+AGG - chr2_4:22027874-22027893 Msa0321030:CDS 35.0%
GGAAGACAACATTCAACAAA+AGG - chr2_4:22027895-22027914 Msa0321030:CDS 35.0%
ACATTCAACAAAAGGAAGCA+TGG - chr2_4:22027903-22027922 Msa0321030:CDS 35.0%
AAGTTGACTGCATAACCATT+AGG - chr2_4:22028052-22028071 Msa0321030:CDS 35.0%
! TGAAAACAATGATGGAGCAA+CGG - chr2_4:22028377-22028396 Msa0321030:intron 35.0%
ACGGTTTATCCATCAAAAGA+AGG - chr2_4:22028396-22028415 Msa0321030:intron 35.0%
AGTTGAAGAAACAAAAGGAC+AGG - chr2_4:22028529-22028548 Msa0321030:intron 35.0%
CTTGGTGAATTCATTCATAC+GGG - chr2_4:22028579-22028598 Msa0321030:intron 35.0%
ATTCATACGGGTGAATTTGA+TGG - chr2_4:22028591-22028610 Msa0321030:intron 35.0%
ACATAACTGATGACTTGAAG+AGG - chr2_4:22027874-22027893 Msa0321030:CDS 35.0%
GGAAGACAACATTCAACAAA+AGG - chr2_4:22027895-22027914 Msa0321030:CDS 35.0%
ACATTCAACAAAAGGAAGCA+TGG - chr2_4:22027903-22027922 Msa0321030:CDS 35.0%
AAGTTGACTGCATAACCATT+AGG - chr2_4:22028052-22028071 Msa0321030:CDS 35.0%
! TGAAAACAATGATGGAGCAA+CGG - chr2_4:22028377-22028396 Msa0321030:intron 35.0%
ACGGTTTATCCATCAAAAGA+AGG - chr2_4:22028396-22028415 Msa0321030:intron 35.0%
AGTTGAAGAAACAAAAGGAC+AGG - chr2_4:22028529-22028548 Msa0321030:intron 35.0%
CTTGGTGAATTCATTCATAC+GGG - chr2_4:22028579-22028598 Msa0321030:intron 35.0%
ATTCATACGGGTGAATTTGA+TGG - chr2_4:22028591-22028610 Msa0321030:intron 35.0%
CATTTCTCTCAACTCTCTCA+CGG - chr2_4:22028265-22028284 Msa0321030:intron 40.0%
ATTTCTCTCAACTCTCTCAC+GGG - chr2_4:22028266-22028285 Msa0321030:intron 40.0%
AGTGAAATCACCACACTTTG+TGG - chr2_4:22028327-22028346 Msa0321030:intron 40.0%
TGCATCAATTCCACAAAGTG+TGG + chr2_4:22028340-22028359 None:intergenic 40.0%
CTTTGTGGAATTGATGCATG+TGG - chr2_4:22028342-22028361 Msa0321030:intron 40.0%
! ATCTGGACTCCTTCTTTTGA+TGG + chr2_4:22028408-22028427 None:intergenic 40.0%
ATCAAAAGAAGGAGTCCAGA+TGG - chr2_4:22028407-22028426 Msa0321030:intron 40.0%
TAAACTTGTCCAACACCATC+TGG + chr2_4:22028425-22028444 None:intergenic 40.0%
! AGATGGTGTTGGACAAGTTT+AGG - chr2_4:22028424-22028443 Msa0321030:intron 40.0%
GTTTAGGAGCTTTCCTGAAT+CGG - chr2_4:22028440-22028459 Msa0321030:intron 40.0%
! CCTTTTCTTTGTTCCGATTC+AGG + chr2_4:22028456-22028475 None:intergenic 40.0%
CCTGAATCGGAACAAAGAAA+AGG - chr2_4:22028453-22028472 Msa0321030:intron 40.0%
GGAACAAAGAAAAGGAAAGC+TGG - chr2_4:22028461-22028480 Msa0321030:intron 40.0%
AAAAGGAAAGCTGGATCATG+AGG - chr2_4:22028470-22028489 Msa0321030:intron 40.0%
AAAGGAAAGCTGGATCATGA+GGG - chr2_4:22028471-22028490 Msa0321030:intron 40.0%
!!! TCCTCTCATCGACTTCTTTT+CGG + chr2_4:22028668-22028687 None:intergenic 40.0%
CATTTCTCTCAACTCTCTCA+CGG - chr2_4:22028265-22028284 Msa0321030:intron 40.0%
ATTTCTCTCAACTCTCTCAC+GGG - chr2_4:22028266-22028285 Msa0321030:intron 40.0%
AGTGAAATCACCACACTTTG+TGG - chr2_4:22028327-22028346 Msa0321030:intron 40.0%
TGCATCAATTCCACAAAGTG+TGG + chr2_4:22028340-22028359 None:intergenic 40.0%
CTTTGTGGAATTGATGCATG+TGG - chr2_4:22028342-22028361 Msa0321030:intron 40.0%
! ATCTGGACTCCTTCTTTTGA+TGG + chr2_4:22028408-22028427 None:intergenic 40.0%
ATCAAAAGAAGGAGTCCAGA+TGG - chr2_4:22028407-22028426 Msa0321030:intron 40.0%
TAAACTTGTCCAACACCATC+TGG + chr2_4:22028425-22028444 None:intergenic 40.0%
! AGATGGTGTTGGACAAGTTT+AGG - chr2_4:22028424-22028443 Msa0321030:intron 40.0%
GTTTAGGAGCTTTCCTGAAT+CGG - chr2_4:22028440-22028459 Msa0321030:intron 40.0%
! CCTTTTCTTTGTTCCGATTC+AGG + chr2_4:22028456-22028475 None:intergenic 40.0%
CCTGAATCGGAACAAAGAAA+AGG - chr2_4:22028453-22028472 Msa0321030:intron 40.0%
GGAACAAAGAAAAGGAAAGC+TGG - chr2_4:22028461-22028480 Msa0321030:intron 40.0%
AAAAGGAAAGCTGGATCATG+AGG - chr2_4:22028470-22028489 Msa0321030:intron 40.0%
AAAGGAAAGCTGGATCATGA+GGG - chr2_4:22028471-22028490 Msa0321030:intron 40.0%
!!! TCCTCTCATCGACTTCTTTT+CGG + chr2_4:22028668-22028687 None:intergenic 40.0%
ACCGAAAAGAAGTCGATGAG+AGG - chr2_4:22028664-22028683 Msa0321030:CDS 45.0%
ACCGAAAAGAAGTCGATGAG+AGG - chr2_4:22028664-22028683 Msa0321030:CDS 45.0%
AGAAGGAGTCCAGATGGTGT+TGG - chr2_4:22028413-22028432 Msa0321030:intron 50.0%
AGAAGGAGTCCAGATGGTGT+TGG - chr2_4:22028413-22028432 Msa0321030:intron 50.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 22027846 22028707 22027846 ID=Msa0321030;Name=Msa0321030
chr2_4 mRNA 22027846 22028707 22027846 ID=Msa0321030-mRNA-1;Parent=Msa0321030;Name=Msa0321030-mRNA-1;_AED=0.29;_eAED=0.29;_QI=0|0|0|1|1|1|2|0|158
chr2_4 exon 22028629 22028707 22028629 ID=Msa0321030-mRNA-1:exon:8084;Parent=Msa0321030-mRNA-1
chr2_4 exon 22027846 22028243 22027846 ID=Msa0321030-mRNA-1:exon:8083;Parent=Msa0321030-mRNA-1
chr2_4 CDS 22028629 22028707 22028629 ID=Msa0321030-mRNA-1:cds;Parent=Msa0321030-mRNA-1
chr2_4 CDS 22027846 22028243 22027846 ID=Msa0321030-mRNA-1:cds;Parent=Msa0321030-mRNA-1
Gene Sequence

>Msa0321030

ATGGCTGATAAAAATATGAGAGTTGACTACATAACTGATGACTTGAAGAGGAAGACAACATTCAACAAAAGGAAGCATGGTATAACTAAGAAGATTAGTGAAATCACCACACTTTGTGGAATTGATGCATGTGGTATAATCTATGATGAAAACAATGATGGAGCAACGGTTTATCCATCAAAAGAAGGAGTCCAGATGGTGTTGGACAAGTTTAGGAGCTTTCCTGAATCGGAACAAAGAAAAGGAAAGCTGGATCATGAGGGCTTTTTGAGACAATCCATAATGAAATCGAAAGAGAAGTTGAAGAAACAAAAGGACAGGTCCAAGAAGAAGAAAATGAATGATCTACTTGGTGAATTCATTCATACGGGTGAATTTGATGGAAATGTGAGCGAGAATGATCTCGAAGATCTATCATTGCTTATTGGTGATTACCGAAAAGAAGTCGATGAGAGGATCGAATCAAGTCAAACCTGA

Protein sequence

>Msa0321030

MADKNMRVDYITDDLKRKTTFNKRKHGITKKISEITTLCGIDACGIIYDENNDGATVYPSKEGVQMVLDKFRSFPESEQRKGKLDHEGFLRQSIMKSKEKLKKQKDRSKKKKMNDLLGEFIHTGEFDGNVSENDLEDLSLLIGDYRKEVDERIESSQT*