AlfalfaGEDB | Msa0321040

M. sativa cultivar zhongmu-4 / Msa0321040

KEGG: K09260
GO: GO:0000003 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0010154 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0022414 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0042995 GO:0043076 GO:0043078 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044424 GO:0044464 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0061458 GO:0065007 GO:0080090 GO:0090406 GO:0097159 GO:0120025 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00319.21

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0321040	GAU41275.1	72.832	173	45	1	1	171	1	173	5.31e-84	258

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0321040	sp\|Q9FJK3\|AGL80_ARATH	54.601	163	69	2	1	161	1	160	7.07e-54	176

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

TFs/TRs:

Gene ID	Type	Classification
Msa0321040	TF	MADS-M-type

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0321040	MtrunA17_Chr8g0351751	73.988	173	42	2	1	171	1	172	1.77e-85	251
Msa0321040	MtrunA17_Chr2g0296831	93.443	122	8	0	1	122	1	122	4.68e-75	220
Msa0321040	MtrunA17_Chr4g0014031	68.421	171	53	1	1	171	1	170	4.41e-74	221
Msa0321040	MtrunA17_Chr3g0110131	66.250	160	53	1	1	160	1	159	3.76e-71	214
Msa0321040	MtrunA17_Chr4g0014321	64.815	162	57	0	1	162	1	162	5.51e-71	214
Msa0321040	MtrunA17_Chr3g0107381	62.821	156	57	1	1	156	1	155	1.45e-70	212
Msa0321040	MtrunA17_Chr4g0013041	66.234	154	50	1	1	154	1	152	1.36e-67	204
Msa0321040	MtrunA17_Chr4g0014121	70.323	155	46	0	1	155	29	183	4.42e-65	197
Msa0321040	MtrunA17_Chr4g0014131	64.912	171	59	1	1	171	1	170	7.83e-65	198
Msa0321040	MtrunA17_Chr3g0107331	50.314	159	78	1	3	161	2	159	6.12e-56	173
Msa0321040	MtrunA17_Chr3g0107351	45.570	158	85	1	3	160	2	158	4.34e-49	156
Msa0321040	MtrunA17_Chr3g0107361	46.203	158	84	1	3	160	2	158	6.42e-48	153
Msa0321040	MtrunA17_Chr3g0107341	44.937	158	86	1	3	160	2	158	1.33e-47	152
Msa0321040	MtrunA17_Chr3g0107461	43.506	154	87	0	1	154	1	154	5.32e-44	143
Msa0321040	MtrunA17_Chr5g0432691	41.250	160	93	1	1	160	1	159	5.92e-42	137
Msa0321040	MtrunA17_Chr2g0283941	41.772	158	92	0	1	158	1	158	1.69e-41	139
Msa0321040	MtrunA17_Chr2g0296851	78.571	98	21	0	74	171	1	98	1.28e-40	132
Msa0321040	MtrunA17_Chr5g0420351	41.401	157	90	2	1	157	1	155	5.59e-39	130
Msa0321040	MtrunA17_Chr4g0031421	44.186	129	72	0	29	157	1	129	3.19e-37	125
Msa0321040	MtrunA17_Chr1g0197261	38.608	158	95	2	1	158	1	156	4.27e-37	125
Msa0321040	MtrunA17_Chr1g0197461	37.821	156	95	2	3	158	2	155	4.55e-35	120
Msa0321040	MtrunA17_Chr1g0197321	35.443	158	101	1	1	158	1	157	4.74e-35	120
Msa0321040	MtrunA17_Chr3g0105791	41.860	129	75	0	29	157	1	129	2.57e-34	117
Msa0321040	MtrunA17_Chr7g0218151	42.208	154	87	2	1	154	1	152	2.78e-34	118
Msa0321040	MtrunA17_Chr1g0188681	38.312	154	93	2	1	154	1	152	1.66e-33	116
Msa0321040	MtrunA17_Chr1g0188661	41.558	154	88	2	1	154	1	152	1.12e-32	114
Msa0321040	MtrunA17_Chr1g0193311	40.000	155	91	2	1	155	1	153	2.59e-32	113
Msa0321040	MtrunA17_Chr1g0188651	42.208	154	85	2	1	154	1	150	1.43e-31	111
Msa0321040	MtrunA17_Chr4g0012761	39.610	154	91	2	1	154	1	152	2.06e-31	110
Msa0321040	MtrunA17_Chr3g0106421	40.945	127	75	0	29	155	1	127	5.62e-30	108
Msa0321040	MtrunA17_Chr2g0296861	36.774	155	97	1	1	155	1	154	4.69e-27	99.8
Msa0321040	MtrunA17_Chr3g0106501	32.308	130	88	0	29	158	1	130	1.92e-23	90.5
Msa0321040	MtrunA17_Chr4g0008701	37.879	132	79	1	1	129	1	132	7.37e-23	94.4
Msa0321040	MtrunA17_Chr3g0128721	36.364	132	83	1	3	133	6	137	3.33e-22	92.4
Msa0321040	MtrunA17_Chr7g0267601	37.121	132	80	1	1	129	1	132	9.18e-22	90.9
Msa0321040	MtrunA17_Chr6g0457851	31.250	112	70	3	1	106	11	121	5.80e-13	65.5
Msa0321040	MtrunA17_Chr1g0212151	26.619	139	85	5	1	127	1	134	4.56e-12	63.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0321040	AT5G48670.1	54.601	163	69	2	1	161	1	160	7.19e-55	176
Msa0321040	AT5G26630.1	47.904	167	86	1	1	167	1	166	6.70e-46	150
Msa0321040	AT1G65300.1	43.030	165	93	1	3	167	2	165	9.44e-42	141
Msa0321040	AT1G65330.1	42.593	162	92	1	3	164	2	162	1.34e-38	133
Msa0321040	AT5G26650.1	41.772	158	91	1	4	161	2	158	1.57e-38	135
Msa0321040	AT5G27960.1	41.772	158	91	1	4	161	2	158	3.11e-38	133
Msa0321040	AT1G31630.1	36.478	159	99	2	3	161	2	158	1.09e-34	124
Msa0321040	AT3G05860.2	39.024	164	95	2	1	162	1	161	4.17e-33	117
Msa0321040	AT5G27810.1	52.688	93	44	0	29	121	1	93	1.11e-32	113
Msa0321040	AT3G05860.3	39.024	164	95	2	1	162	1	161	2.30e-32	116
Msa0321040	AT3G05860.1	39.024	164	95	2	1	162	1	161	3.12e-32	116
Msa0321040	AT1G31640.1	39.073	151	90	2	3	153	2	150	6.50e-31	116
Msa0321040	AT2G28700.1	35.948	153	91	2	1	150	1	149	1.13e-27	105
Msa0321040	AT5G26580.1	34.783	161	90	2	1	161	1	146	9.83e-27	103
Msa0321040	AT1G22590.2	30.061	163	109	2	1	163	1	158	2.15e-21	85.9
Msa0321040	AT2G40210.1	37.879	132	82	0	1	132	1	132	9.87e-20	85.1
Msa0321040	AT5G06500.1	33.858	127	84	0	1	127	1	127	1.25e-19	82.8
Msa0321040	AT5G55690.1	39.362	94	50	2	1	87	1	94	1.68e-13	67.0
Msa0321040	AT5G55690.2	39.362	94	50	2	1	87	1	94	1.68e-13	67.0
Msa0321040	AT5G58890.1	40.179	112	58	4	1	105	1	110	7.15e-13	65.5
Msa0321040	AT2G15660.1	23.871	155	100	3	11	163	18	156	7.22e-11	59.3

Find 32 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TATCAATATCCTTCAAATTT+TGG	0.165838	2_4:+22036991	None:intergenic
ATGAACCTCAACCTCATATT+TGG	0.258532	2_4:-22037271	Msa0321040:CDS
TTCTTGCTCTTCTCTAATTC+AGG	0.259904	2_4:+22037200	None:intergenic
CACAATCATGACTAGGAAAA+AGG	0.298378	2_4:-22037429	None:intergenic
CATCACCATGGGGAGTTCAT+AGG	0.334299	2_4:-22037247	Msa0321040:CDS
TGAAGCATGTGCTATAGTTC+AGG	0.354873	2_4:-22037300	Msa0321040:CDS
GTCGATTGACCAAAATTTGA+AGG	0.406050	2_4:-22037000	Msa0321040:CDS
GAAGGATATTGATAGAAGAT+TGG	0.427774	2_4:-22036982	Msa0321040:CDS
ATAGGGTGCTCTCAAAATTC+AGG	0.434987	2_4:-22037229	Msa0321040:CDS
TTTGCGTCATGAATGTCTCT+TGG	0.452946	2_4:+22037168	None:intergenic
TTGGAAGAAGTGGATAATAA+TGG	0.455688	2_4:-22036963	Msa0321040:CDS
GGAAATCAACCTATAAGAAA+AGG	0.476350	2_4:-22037373	Msa0321040:CDS
CTCATATTTGGCCATCACCA+TGG	0.499740	2_4:-22037259	Msa0321040:CDS
TCATATTTGGCCATCACCAT+GGG	0.503042	2_4:-22037258	Msa0321040:CDS
GATGAACTGAGCACACTTTG+TGG	0.503919	2_4:-22037326	Msa0321040:CDS
TTGATTTGAATGATCTTGCT+TGG	0.508045	2_4:-22037022	Msa0321040:CDS
ATCACCATGGGGAGTTCATA+GGG	0.514521	2_4:-22037246	Msa0321040:CDS
GAAGCATGTGCTATAGTTCA+GGG	0.519428	2_4:-22037299	Msa0321040:CDS
TGATAGAAGATTGGAAGAAG+TGG	0.523326	2_4:-22036973	Msa0321040:CDS
CATGGTGATGGCCAAATATG+AGG	0.531321	2_4:+22037260	None:intergenic
AGAGACATTCATGACGCAAA+GGG	0.534224	2_4:-22037165	Msa0321040:CDS
TGAGCAGTCATGACTTGACT+TGG	0.542884	2_4:+22036936	None:intergenic
TCATTGTCAATGACGCTGCT+CGG	0.556837	2_4:-22037394	Msa0321040:CDS
CTATGAACTCCCCATGGTGA+TGG	0.559546	2_4:+22037248	None:intergenic
AGCAGCGTCATTGACAATGA+AGG	0.575999	2_4:+22037397	None:intergenic
CACAACTCACAATCATGACT+AGG	0.590575	2_4:-22037436	None:intergenic
AAGAGACATTCATGACGCAA+AGG	0.614539	2_4:-22037166	Msa0321040:CDS
GATGGCCAAATATGAGGTTG+AGG	0.620009	2_4:+22037266	None:intergenic
AAAACTAAGGAAGGGAAACA+GGG	0.623030	2_4:-22037117	Msa0321040:CDS
ATATGAGGTTGAGGTTCATG+AGG	0.635051	2_4:+22037275	None:intergenic
AGCACCCTATGAACTCCCCA+TGG	0.654106	2_4:+22037242	None:intergenic
CATATTTGGCCATCACCATG+GGG	0.723900	2_4:-22037257	Msa0321040:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATTATTCATCCTTTTCTTAT+AGG	+	chr2_4:22036990-22037009	None:intergenic	20.0%
!!!	TATCAATATCCTTCAAATTT+TGG	+	chr2_4:22037363-22037382	None:intergenic	20.0%
!	AAAGTGAAAAAACTAAGGAA+GGG	-	chr2_4:22037226-22037245	Msa0321040:CDS	25.0%
	GGAAATCAACCTATAAGAAA+AGG	-	chr2_4:22036978-22036997	Msa0321040:CDS	30.0%
	AGGAGAAAGTGAAAAAACTA+AGG	-	chr2_4:22037221-22037240	Msa0321040:CDS	30.0%
	GAAAGTGAAAAAACTAAGGA+AGG	-	chr2_4:22037225-22037244	Msa0321040:CDS	30.0%
!	TTGATTTGAATGATCTTGCT+TGG	-	chr2_4:22037329-22037348	Msa0321040:CDS	30.0%
	GAAGGATATTGATAGAAGAT+TGG	-	chr2_4:22037369-22037388	Msa0321040:CDS	30.0%
	TTGGAAGAAGTGGATAATAA+TGG	-	chr2_4:22037388-22037407	Msa0321040:CDS	30.0%
	ATGAACCTCAACCTCATATT+TGG	-	chr2_4:22037080-22037099	Msa0321040:CDS	35.0%
	TTCTTGCTCTTCTCTAATTC+AGG	+	chr2_4:22037154-22037173	None:intergenic	35.0%
	AAAAACTAAGGAAGGGAAAC+AGG	-	chr2_4:22037233-22037252	Msa0321040:CDS	35.0%
	AAAACTAAGGAAGGGAAACA+GGG	-	chr2_4:22037234-22037253	Msa0321040:CDS	35.0%
	GTCGATTGACCAAAATTTGA+AGG	-	chr2_4:22037351-22037370	Msa0321040:CDS	35.0%
	TGATAGAAGATTGGAAGAAG+TGG	-	chr2_4:22037378-22037397	Msa0321040:CDS	35.0%
	TGAAGCATGTGCTATAGTTC+AGG	-	chr2_4:22037051-22037070	Msa0321040:CDS	40.0%
	GAAGCATGTGCTATAGTTCA+GGG	-	chr2_4:22037052-22037071	Msa0321040:CDS	40.0%
	ATATGAGGTTGAGGTTCATG+AGG	+	chr2_4:22037079-22037098	None:intergenic	40.0%
	TCATATTTGGCCATCACCAT+GGG	-	chr2_4:22037093-22037112	Msa0321040:CDS	40.0%
!	ATAGGGTGCTCTCAAAATTC+AGG	-	chr2_4:22037122-22037141	Msa0321040:CDS	40.0%
	TTTGCGTCATGAATGTCTCT+TGG	+	chr2_4:22037186-22037205	None:intergenic	40.0%
	AAGAGACATTCATGACGCAA+AGG	-	chr2_4:22037185-22037204	Msa0321040:CDS	40.0%
	AGAGACATTCATGACGCAAA+GGG	-	chr2_4:22037186-22037205	Msa0321040:CDS	40.0%
	AGCAGCGTCATTGACAATGA+AGG	+	chr2_4:22036957-22036976	None:intergenic	45.0%
	TCATTGTCAATGACGCTGCT+CGG	-	chr2_4:22036957-22036976	Msa0321040:CDS	45.0%
	GATGAACTGAGCACACTTTG+TGG	-	chr2_4:22037025-22037044	Msa0321040:CDS	45.0%
	GATGGCCAAATATGAGGTTG+AGG	+	chr2_4:22037088-22037107	None:intergenic	45.0%
!!	CATGGTGATGGCCAAATATG+AGG	+	chr2_4:22037094-22037113	None:intergenic	45.0%
	CTCATATTTGGCCATCACCA+TGG	-	chr2_4:22037092-22037111	Msa0321040:CDS	45.0%
	CATATTTGGCCATCACCATG+GGG	-	chr2_4:22037094-22037113	Msa0321040:CDS	45.0%
	ATCACCATGGGGAGTTCATA+GGG	-	chr2_4:22037105-22037124	Msa0321040:CDS	45.0%
!!	CATGACGCAAAGGGTTTTGA+AGG	-	chr2_4:22037195-22037214	Msa0321040:CDS	45.0%
!!!	GCAAAGGGTTTTGAAGGCTA+AGG	-	chr2_4:22037201-22037220	Msa0321040:CDS	45.0%
	TGAGCAGTCATGACTTGACT+TGG	+	chr2_4:22037418-22037437	None:intergenic	45.0%
	CTATGAACTCCCCATGGTGA+TGG	+	chr2_4:22037106-22037125	None:intergenic	50.0%
	CATCACCATGGGGAGTTCAT+AGG	-	chr2_4:22037104-22037123	Msa0321040:CDS	50.0%
	AGCACCCTATGAACTCCCCA+TGG	+	chr2_4:22037112-22037131	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr2_4	gene	22036929	22037444	22036929	ID=Msa0321040;Name=Msa0321040
chr2_4	mRNA	22036929	22037444	22036929	ID=Msa0321040-mRNA-1;Parent=Msa0321040;Name=Msa0321040-mRNA-1;_AED=0.27;_eAED=0.27;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|171
chr2_4	exon	22036929	22037444	22036929	ID=Msa0321040-mRNA-1:exon:8082;Parent=Msa0321040-mRNA-1
chr2_4	CDS	22036929	22037444	22036929	ID=Msa0321040-mRNA-1:cds;Parent=Msa0321040-mRNA-1

Gene Sequence

>Msa0321040

ATGACTAGGAAAAAGGTGAAACTCGCCTTCATTGTCAATGACGCTGCTCGGAAATCAACCTATAAGAAAAGGATGAATAATTTATTGAAGAAAGTCGATGAACTGAGCACACTTTGTGGTATTGAAGCATGTGCTATAGTTCAGGGTCCTCATGAACCTCAACCTCATATTTGGCCATCACCATGGGGAGTTCATAGGGTGCTCTCAAAATTCAGGACTATGCCTGAATTAGAGAAGAGCAAGAAAATGATGAACCAAGAGACATTCATGACGCAAAGGGTTTTGAAGGCTAAGGAGAAAGTGAAAAAACTAAGGAAGGGAAACAGGGAGCAAGAGATGACAATGATCATGTTTCAATGTCTTAATGCTGAAAAAATTGTGCACGATAATATGTCAATGATTGATTTGAATGATCTTGCTTGGTCGATTGACCAAAATTTGAAGGATATTGATAGAAGATTGGAAGAAGTGGATAATAATGGTCATCCAAGTCAAGTCATGACTGCTCAAAGTTAA

Protein sequence

>Msa0321040

MTRKKVKLAFIVNDAARKSTYKKRMNNLLKKVDELSTLCGIEACAIVQGPHEPQPHIWPSPWGVHRVLSKFRTMPELEKSKKMMNQETFMTQRVLKAKEKVKKLRKGNREQEMTMIMFQCLNAEKIVHDNMSMIDLNDLAWSIDQNLKDIDRRLEEVDNNGHPSQVMTAQS*