AlfalfaGEDB | Msa0321810

M. sativa cultivar zhongmu-4 / Msa0321810

KEGG: K04733
GO: GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564
PFAM: PF07714.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0321810	XP_013463430.1	83.871	62	10	0	69	130	2	63	1.20e-22	105
Msa0321810	XP_013463430.1	98.611	72	1	0	135	206	130	201	1.23e-37	145

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0321810	sp\|O64784\|Y1136_ARATH	58.333	72	29	1	135	206	514	584	2.32e-21	94.7

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0321810	MtrunA17_Chr2g0299041	98.611	72	1	0	135	206	130	201	1.13e-41	145
Msa0321810	MtrunA17_Chr2g0299041	83.871	62	10	0	69	130	2	63	1.11e-26	105
Msa0321810	MtrunA17_Chr4g0071371	66.667	90	28	1	117	206	136	223	1.41e-33	124
Msa0321810	MtrunA17_Chr7g0236341	59.722	72	28	1	135	206	535	605	6.43e-21	90.1
Msa0321810	MtrunA17_Chr2g0318831	61.111	72	27	1	135	206	519	589	9.57e-21	89.7
Msa0321810	MtrunA17_Chr7g0236471	59.722	72	28	1	135	206	357	427	1.34e-20	89.0
Msa0321810	MtrunA17_Chr4g0042341	61.111	72	27	1	135	206	540	610	1.88e-20	89.0
Msa0321810	MtrunA17_Chr2g0318811	58.333	72	29	1	135	206	517	587	2.24e-20	88.6
Msa0321810	MtrunA17_Chr7g0236561	58.333	72	29	1	135	206	522	592	2.38e-20	88.6
Msa0321810	MtrunA17_Chr7g0236531	56.944	72	30	1	135	206	55	125	3.57e-20	87.0

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0321810	AT1G61360.1	58.333	72	29	1	135	206	514	584	2.36e-22	94.7
Msa0321810	AT1G61360.2	58.333	72	29	1	135	206	433	503	2.64e-22	94.4
Msa0321810	AT1G61360.3	58.333	72	29	1	135	206	433	503	2.64e-22	94.4
Msa0321810	AT1G61360.4	58.333	72	29	1	135	206	433	503	2.64e-22	94.4
Msa0321810	AT1G61400.2	56.944	72	30	1	135	206	516	586	4.32e-22	94.0
Msa0321810	AT1G61440.3	56.944	72	30	1	135	206	499	569	4.59e-22	94.0
Msa0321810	AT1G61400.1	56.944	72	30	1	135	206	516	586	4.66e-22	94.0
Msa0321810	AT1G61440.1	56.944	72	30	1	135	206	494	564	4.81e-22	94.0
Msa0321810	AT1G61440.2	54.795	73	32	1	134	206	523	594	1.14e-21	92.8
Msa0321810	AT4G27300.1	65.278	72	24	1	135	206	516	586	1.64e-21	92.4

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Find 42 sgRNAs with CRISPR-Local

Find 321 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACGATCACCAAATCCAAT+GGG	0.549660	2_4:-23636391	Msa0321810:CDS
AAATTAGGTATAGGAGGGTT+TGG	0.382128	2_4:+23638812	None:intergenic
AACTGCTTTCCCATTGGATT+TGG	0.341589	2_4:+23636382	None:intergenic
ACAATGTTCTTTGTCCTTGG+AGG	0.457612	2_4:-23636472	Msa0321810:CDS
ACATAATCTTGAAGCTTAGC+AGG	0.500590	2_4:+23638728	None:intergenic
ACATGTAGAGTATCATAATC+AGG	0.446906	2_4:-23638897	None:intergenic
AGGAGGGTTTGGAATAGGAG+AGG	0.608188	2_4:+23638823	None:intergenic
AGGGAAAGTTGGAAGATGGA+AGG	0.547330	2_4:-23634440	Msa0321810:CDS
AGTTTGAACCTCTTCATATA+TGG	0.281265	2_4:-23634246	None:intergenic
ATATGAAGAGGTTCAAACTT+CGG	0.537983	2_4:+23634250	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GGAGAATCATTATGATTGAT+AGG	+	chr2_4:23634293-23634312	None:intergenic	30.0%
TCTATACGATCAGGTAAATT+AGG	+	chr2_4:23634341-23634360	None:intergenic	30.0%
GAAGAAGTGAAATTAGACAA+AGG	+	chr2_4:23634485-23634504	None:intergenic	30.0%
ATAACCTCTTCATAGGATTT+GGG	+	chr2_4:23634566-23634585	None:intergenic	30.0%
CTTTAGTAAAGAATGGTACA+TGG	-	chr2_4:23634630-23634649	Msa0321810:intron	30.0%
TCATACTTCTAAACCTAATC+AGG	-	chr2_4:23634955-23634974	Msa0321810:intron	30.0%
TGATGCATCTTTCAAAATCA+AGG	-	chr2_4:23635204-23635223	Msa0321810:intron	30.0%
AGTTGTATATGTGATGACTT+AGG	+	chr2_4:23635598-23635617	None:intergenic	30.0%
AAGTAATGTCACAAATGAAG+AGG	-	chr2_4:23636147-23636166	Msa0321810:intron	30.0%
ACTAGCATATAGTTAGTGTA+TGG	-	chr2_4:23636246-23636265	Msa0321810:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_4	gene	23634248	23638909	23634248	ID=Msa0321810;Name=Msa0321810
chr2_4	mRNA	23634248	23638909	23634248	ID=Msa0321810-mRNA-1;Parent=Msa0321810;Name=Msa0321810-mRNA-1;_AED=0.41;_eAED=0.41;_QI=0\|0\|0\|0.66\|1\|1\|3\|0\|206
chr2_4	exon	23638687	23638909	23638687	ID=Msa0321810-mRNA-1:exon:8585;Parent=Msa0321810-mRNA-1
chr2_4	exon	23636372	23636553	23636372	ID=Msa0321810-mRNA-1:exon:8584;Parent=Msa0321810-mRNA-1
chr2_4	exon	23634248	23634460	23634248	ID=Msa0321810-mRNA-1:exon:8583;Parent=Msa0321810-mRNA-1
chr2_4	CDS	23638687	23638909	23638687	ID=Msa0321810-mRNA-1:cds;Parent=Msa0321810-mRNA-1
chr2_4	CDS	23636372	23636553	23636372	ID=Msa0321810-mRNA-1:cds;Parent=Msa0321810-mRNA-1
chr2_4	CDS	23634248	23634460	23634248	ID=Msa0321810-mRNA-1:cds;Parent=Msa0321810-mRNA-1

Gene Sequence

>Msa0321810

TATCATAATCAGGATTCTTTCTCTGATTCTCAACCTATTCTTCCTATCAATCATAATGATTCTCCCTCTCCTATTCCAAACCCTCCTATACCTAATTTACCTGATCGTATAGATGATGTTGAGCCTGTTCTTAGAAGGTCAGAAAGAGTCAAAACTGTTCCTGCTAAGCTTCAAGATTATGTCTGTAACTCCTTCACAGATCAACCAACTTCATCTTCTTCAGGAGGAGCTTCTTTAGCCTCAAATGACGAGTCAAAGAAGTCAAGTGAACACAAACGTGAAACAATGTTCTTTGTCCTTGGAGGAATAGTAGTGGTCACCATAAAATTTATTCTTGGTTGGATTGTATTTAGAAAACGTGTGAAACGATCACCAAATCCAATGGGAAAGCAGTTCCAAATGAAGGGAAAGTTGGAAGATGGAAGGATCATTGCTGTTAAGGCATTGTCTCTTAACAAATCTCAACAAGGAGAAAGAGAATTTTTGGCTGAGGTTAAATTGATTACAAGTATCCAACACAAAAATTTGGTTCGCCTTCTTGGAAGTTGTATAGATGGTCCTCAAAGGATACTTATTTATGAATACATGAAAAACCGAAGTTTGAACCTCTTCATATAT

Protein sequence

>Msa0321810

YHNQDSFSDSQPILPINHNDSPSPIPNPPIPNLPDRIDDVEPVLRRSERVKTVPAKLQDYVCNSFTDQPTSSSSGGASLASNDESKKSSEHKRETMFFVLGGIVVVTIKFILGWIVFRKRVKRSPNPMGKQFQMKGKLEDGRIIAVKALSLNKSQQGEREFLAEVKLITSIQHKNLVRLLGSCIDGPQRILIYEYMKNRSLNLFIY