AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0322340

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0322340 MtrunA17_Chr2g0299621 94.369 586 31 1 1 586 1 584 0.0 1083
Msa0322340 MtrunA17_Chr2g0291741 43.113 559 285 11 18 558 21 564 6.49e-143 426
Msa0322340 MtrunA17_Chr5g0442661 40.490 531 290 9 50 563 50 571 1.15e-123 377
Msa0322340 MtrunA17_Chr1g0171771 31.267 371 213 8 67 402 24 387 8.47e-38 146
Msa0322340 MtrunA17_Chr7g0256321 31.183 372 213 7 67 402 24 388 1.15e-37 145
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0322340 AT1G64280.1 53.831 522 218 5 51 557 49 562 0.0 546
Msa0322340 AT4G26120.2 49.391 575 257 9 12 569 16 573 2.78e-175 509
Msa0322340 AT4G26120.1 49.391 575 257 9 12 569 15 572 3.06e-175 509
Msa0322340 AT5G45110.1 42.344 529 271 11 50 556 43 559 5.93e-122 372
Msa0322340 AT4G19660.1 40.619 549 283 11 31 555 22 551 2.13e-121 370
Msa0322340 AT4G19660.2 38.889 576 282 12 31 555 22 578 2.60e-116 358
Msa0322340 AT5G45110.2 41.529 484 249 11 50 514 43 511 6.85e-108 334
Msa0322340 AT3G57130.1 31.733 375 203 10 67 402 26 386 2.95e-40 153
Msa0322340 AT2G41370.1 30.348 402 216 10 54 402 3 393 3.27e-36 142

Find 100 sgRNAs with CRISPR-Local

Find 149 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTGAATTTACTACGAATT+TGG 0.069114 2_4:-24765844 Msa0322340:CDS
TGCGAGGAGTAGTTTCTTTA+AGG 0.142531 2_4:-24767365 Msa0322340:CDS
CACCTTTCAAATTTCTGAAT+TGG 0.280768 2_4:-24767113 Msa0322340:CDS
GCGCTTCACTTGTCGATCTT+CGG 0.290085 2_4:+24765253 None:intergenic
TGAATTGGTTGCTCTTTATC+AGG 0.291540 2_4:-24767098 Msa0322340:intron
ACTAGACATTCTTGACAAAA+TGG 0.299539 2_4:-24766795 Msa0322340:CDS
TCTGAATTTACTACGAATTT+GGG 0.306050 2_4:-24765843 Msa0322340:CDS
ATGTTTGTCTAGAAGATTTA+CGG 0.318420 2_4:+24766577 None:intergenic
TATATTACTTGTTTCCCTAT+AGG 0.324521 2_4:+24766635 None:intergenic
ATCTAAAGCTCTATGTATTC+TGG 0.325893 2_4:+24766550 None:intergenic
CGCTTCACTTGTCGATCTTC+GGG 0.329375 2_4:+24765254 None:intergenic
CCTTCTGAACTCACTTTAGA+TGG 0.333903 2_4:-24766290 Msa0322340:CDS
AAATCACTGCCTCAGCCTAT+AGG 0.337007 2_4:-24766650 Msa0322340:CDS
GCTACAAGAAGTTCTTAATA+AGG 0.338445 2_4:-24765216 Msa0322340:CDS
ACTACCGAGCTTCTTGATCT+TGG 0.358468 2_4:-24766422 Msa0322340:CDS
TCGATTGTGTATCGAGATAT+TGG 0.362910 2_4:-24766180 Msa0322340:CDS
AATTCAGATGTGCCATCAAT+TGG 0.381830 2_4:+24765858 None:intergenic
AATCACTGCCTCAGCCTATA+GGG 0.387675 2_4:-24766649 Msa0322340:CDS
GAAGTAGTGATAGCTGAATT+TGG 0.388122 2_4:+24767538 None:intergenic
GTGACTAAGGATTATGATGT+TGG 0.390092 2_4:-24767298 Msa0322340:CDS
ATTGTTTACACTTTGGTTGT+TGG 0.395598 2_4:-24767177 Msa0322340:CDS
TCATCCTCATCCACGTCAAT+AGG 0.401640 2_4:-24765146 Msa0322340:CDS
CATACTCCTCTTTGTCCTCA+TGG 0.417825 2_4:+24765189 None:intergenic
TCATCATGAGCAGTGGAGTT+GGG 0.420958 2_4:-24765368 Msa0322340:intron
AGAAACTTCTCGACCATCTC+TGG 0.426255 2_4:+24767408 None:intergenic
TAAAAGAGGAGCATCTAATT+CGG 0.429046 2_4:-24765773 Msa0322340:CDS
AAAGAGGAGTATGATAAATC+TGG 0.432648 2_4:-24765179 Msa0322340:CDS
CAGATGATGTAGAATTAGTA+AGG 0.438766 2_4:-24766526 Msa0322340:CDS
GTTCTGCGTTATTTGTATAG+TGG 0.456294 2_4:-24767256 Msa0322340:CDS
ATCATACTATTATTAGCCCT+TGG 0.460763 2_4:+24765119 None:intergenic
TATCTTTCTCTTGCTAAGGC+AGG 0.461159 2_4:-24766119 Msa0322340:CDS
CCTATCGCAGCTTGCATGCA+TGG 0.468067 2_4:-24765288 Msa0322340:CDS
TGGTTACCAGAGGACAACTA+TGG 0.474344 2_4:-24765820 Msa0322340:CDS
ATGGATATCAATCCAATTGA+TGG 0.475189 2_4:-24765870 Msa0322340:CDS
TGACTAAGGATTATGATGTT+GGG 0.476464 2_4:-24767297 Msa0322340:CDS
ACGGTTCTTCCCTCGTTGCT+CGG 0.483600 2_4:-24765342 Msa0322340:CDS
TGAAGCATGAAATCAACAAC+AGG 0.485589 2_4:+24767154 None:intergenic
CTTTCTATAGCAAACATTTG+TGG 0.486023 2_4:-24766749 Msa0322340:CDS
GTCATCATGAGCAGTGGAGT+TGG 0.495089 2_4:-24765369 Msa0322340:intron
ATCTTTCTCTTGCTAAGGCA+GGG 0.498490 2_4:-24766118 Msa0322340:CDS
ATCACAACCTTTGCTTCCAT+CGG 0.509584 2_4:+24765891 None:intergenic
GCAACTGAAGGAAGTGACTA+AGG 0.511330 2_4:-24767311 Msa0322340:CDS
GAGCAGTGGAGTTGGGGAAA+CGG 0.514567 2_4:-24765361 Msa0322340:intron
GGCATATCTTTCTCTTGCTA+AGG 0.517290 2_4:-24766123 Msa0322340:CDS
ATTGGAGCAAGCTGAGAGAA+GGG 0.524671 2_4:-24766162 Msa0322340:CDS
GATGATGATTGTTTACACTT+TGG 0.524715 2_4:-24767184 Msa0322340:CDS
ATTGTATCTTTGTTAACGAA+GGG 0.525145 2_4:-24766320 Msa0322340:CDS
GTCGAGAAGTTTCTGTGCAC+CGG 0.525175 2_4:-24767399 Msa0322340:CDS
TCAAGTCCATAGTTGTCCTC+TGG 0.525929 2_4:+24765814 None:intergenic
GGAAGTGTTGAACAAGATCA+TGG 0.534097 2_4:-24765321 Msa0322340:CDS
GGCAATAGATGACATGTTGA+CGG 0.536657 2_4:-24766774 Msa0322340:CDS
GTAATGATGTACTTGAGAAG+TGG 0.540852 2_4:-24767631 Msa0322340:CDS
ACTTGTTTCCCTATAGGCTG+AGG 0.543182 2_4:+24766641 None:intergenic
TATTGGAGCAAGCTGAGAGA+AGG 0.543360 2_4:-24766163 Msa0322340:CDS
TATTATAAATCTACTGAGGA+AGG 0.543578 2_4:-24766221 Msa0322340:CDS
ATGAAGGCGTTGTCTAGAGC+AGG 0.543907 2_4:-24765750 Msa0322340:intron
CATTCCTATTGACGTGGATG+AGG 0.544059 2_4:+24765142 None:intergenic
GGCACCATTTAAAATAAAAG+AGG 0.551112 2_4:-24765787 Msa0322340:CDS
TATTGTATCTTTGTTAACGA+AGG 0.551812 2_4:-24766321 Msa0322340:CDS
AAGATCAAGAAGCTCGGTAG+TGG 0.558220 2_4:+24766424 None:intergenic
GGAGCATCTAATTCGGATGA+AGG 0.559886 2_4:-24765766 Msa0322340:CDS
TGCAAGCTGCGATAGGTCAT+CGG 0.560452 2_4:+24765295 None:intergenic
CATCATGAGCAGTGGAGTTG+GGG 0.560586 2_4:-24765367 Msa0322340:intron
CTATCGCAGCTTGCATGCAT+GGG 0.560772 2_4:-24765287 Msa0322340:CDS
GCAAAGATCATTGCCAGAGA+TGG 0.562499 2_4:-24767421 Msa0322340:CDS
CCATCTAAAGTGAGTTCAGA+AGG 0.563795 2_4:+24766290 None:intergenic
AGATATGCCTCACCATGTAA+AGG 0.567064 2_4:+24766137 None:intergenic
AACCAATTCAGAAATTTGAA+AGG 0.567258 2_4:+24767111 None:intergenic
AGAAACGATGCTGCAACTGA+AGG 0.567744 2_4:-24767323 Msa0322340:CDS
CCATGCATGCAAGCTGCGAT+AGG 0.568643 2_4:+24765288 None:intergenic
TCTTTCTCTTGCTAAGGCAG+GGG 0.569008 2_4:-24766117 Msa0322340:CDS
GAGAGAAGGGAGCCTTTACA+TGG 0.569857 2_4:-24766149 Msa0322340:CDS
TAAAGATGTTTGTGTTTGTG+TGG 0.576566 2_4:-24767206 Msa0322340:CDS
TTTGTTTCCGATGGAAGCAA+AGG 0.576921 2_4:-24765898 Msa0322340:CDS
GAATTTACTACGAATTTGGG+TGG 0.585360 2_4:-24765840 Msa0322340:CDS
GACAACTATGGACTTGAATG+AGG 0.586182 2_4:-24765808 Msa0322340:CDS
TCTGGCAATGATCTTTGCAT+CGG 0.586644 2_4:+24767426 None:intergenic
AAGCTATATTGTCTGATAGA+CGG 0.587341 2_4:+24767482 None:intergenic
AGGCAGTGATTTCTCAAGAG+TGG 0.590264 2_4:+24766661 None:intergenic
GATGGAAGCAAAGGTTGTGA+TGG 0.592814 2_4:-24765889 Msa0322340:CDS
CCTTGGCATTCCTATTGACG+TGG 0.593232 2_4:+24765136 None:intergenic
AACGAAATCAGCAACAACAC+TGG 0.604355 2_4:-24767592 Msa0322340:CDS
CCACGTCAATAGGAATGCCA+AGG 0.605123 2_4:-24765136 Msa0322340:CDS
TCCTCGCAGACAAAATGCAC+CGG 0.610061 2_4:+24767380 None:intergenic
ATATACAAACGCAAACAACT+AGG 0.616094 2_4:-24766617 Msa0322340:CDS
AAAGCGTAGTACGAAAGCTG+TGG 0.616351 2_4:-24766246 Msa0322340:CDS
AAGGGAGCCTTTACATGGTG+AGG 0.619314 2_4:-24766144 Msa0322340:CDS
ATATTGTCTGATAGACGGAT+CGG 0.631580 2_4:+24767487 None:intergenic
GGTCTTCCATGAGGACAAAG+AGG 0.638424 2_4:-24765195 Msa0322340:CDS
AGTGAAGCGCAGACGATACG+CGG 0.639553 2_4:-24765240 Msa0322340:CDS
AAGTCCAAGATCAAGAAGCT+CGG 0.641506 2_4:+24766418 None:intergenic
CGAATTTGGGTGGTTACCAG+AGG 0.643360 2_4:-24765830 Msa0322340:CDS
CACGTCAATAGGAATGCCAA+GGG 0.650742 2_4:-24765135 Msa0322340:CDS
AGGACACACTACTCTAGATG+AGG 0.655016 2_4:-24766489 Msa0322340:CDS
TTGTCTGATAGACGGATCGG+TGG 0.664514 2_4:+24767490 None:intergenic
TCTTAATAAGGTCTTCCATG+AGG 0.672788 2_4:-24765204 Msa0322340:CDS
TCAACACTTCCGAGCAACGA+GGG 0.684586 2_4:+24765333 None:intergenic
TTCAACACTTCCGAGCAACG+AGG 0.701823 2_4:+24765332 None:intergenic
GGATTATTATAAATCTACTG+AGG 0.714422 2_4:-24766225 Msa0322340:CDS
TTGTTGTACCTAGAAAACCG+AGG 0.809311 2_4:-24766077 Msa0322340:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGTTAAAGTAATTTCATA+GGG - chr2_4:24765704-24765723 Msa0322340:intron 15.0%
!! ATGAAATTAAATTAACTGAA+AGG + chr2_4:24765850-24765869 None:intergenic 15.0%
!!! AAGATTTTTATGATTTACAT+CGG + chr2_4:24766340-24766359 None:intergenic 15.0%
!! ATGTAAATCATAAAAATCTT+AGG - chr2_4:24766340-24766359 Msa0322340:CDS 15.0%
!! TGTAAATCATAAAAATCTTA+GGG - chr2_4:24766341-24766360 Msa0322340:CDS 15.0%
!!! AACAAAGATACAATAATTTT+CGG + chr2_4:24766405-24766424 None:intergenic 15.0%
!!! CATTTTAGATTTAAATACAA+TGG + chr2_4:24767088-24767107 None:intergenic 15.0%
!! TGCAAAATTTAACAAATAAA+TGG + chr2_4:24767310-24767329 None:intergenic 15.0%
!!! TTTCTTTAAGGATGTTTTTA+AGG - chr2_4:24765378-24765397 Msa0322340:intron 20.0%
!!! TTCTTTAAGGATGTTTTTAA+GGG - chr2_4:24765379-24765398 Msa0322340:intron 20.0%
!! GAAAGTTAAAGTAATTTCAT+AGG - chr2_4:24765703-24765722 Msa0322340:intron 20.0%
!! AAGTTAAAGTAATTTCATAG+GGG - chr2_4:24765705-24765724 Msa0322340:intron 20.0%
!! GTAAATCATAAAAATCTTAG+GGG - chr2_4:24766342-24766361 Msa0322340:CDS 20.0%
!! TCAACATTTATTGTGAAATT+CGG + chr2_4:24766771-24766790 None:intergenic 20.0%
!! TCAAAAATAAAACGAAAGAT+GGG + chr2_4:24767023-24767042 None:intergenic 20.0%
!!! ATTTTTGAGTACTTTCTTAA+AGG - chr2_4:24767035-24767054 Msa0322340:intron 20.0%
!! GTAAAGAAAGAAAAATAGAA+TGG + chr2_4:24767114-24767133 None:intergenic 20.0%
!! TTTCATTATACGTTAGATAT+CGG - chr2_4:24767281-24767300 Msa0322340:CDS 20.0%
! AACCAATTCAGAAATTTGAA+AGG + chr2_4:24765623-24765642 None:intergenic 25.0%
! TATATTACTTGTTTCCCTAT+AGG + chr2_4:24766099-24766118 None:intergenic 25.0%
! ATGTTTGTCTAGAAGATTTA+CGG + chr2_4:24766157-24766176 None:intergenic 25.0%
! TATTGTATCTTTGTTAACGA+AGG - chr2_4:24766410-24766429 Msa0322340:CDS 25.0%
! ATTGTATCTTTGTTAACGAA+GGG - chr2_4:24766411-24766430 Msa0322340:CDS 25.0%
! GGATTATTATAAATCTACTG+AGG - chr2_4:24766506-24766525 Msa0322340:CDS 25.0%
! TATTATAAATCTACTGAGGA+AGG - chr2_4:24766510-24766529 Msa0322340:CDS 25.0%
! CAACATTTATTGTGAAATTC+GGG + chr2_4:24766770-24766789 None:intergenic 25.0%
! ATCTGAATTTACTACGAATT+TGG - chr2_4:24766887-24766906 Msa0322340:intron 25.0%
! TCTGAATTTACTACGAATTT+GGG - chr2_4:24766888-24766907 Msa0322340:intron 25.0%
!!! TGCTCCTCTTTTATTTTAAA+TGG + chr2_4:24766951-24766970 None:intergenic 25.0%
! CTCAAAAATAAAACGAAAGA+TGG + chr2_4:24767024-24767043 None:intergenic 25.0%
! AGTTCGAAAACAATTGATAT+AGG - chr2_4:24767251-24767270 Msa0322340:CDS 25.0%
! GTTCGAAAACAATTGATATA+GGG - chr2_4:24767252-24767271 Msa0322340:CDS 25.0%
! ATCTAACGTATAATGAAAAC+TGG + chr2_4:24767279-24767298 None:intergenic 25.0%
AAGCTATATTGTCTGATAGA+CGG + chr2_4:24765252-24765271 None:intergenic 30.0%
TGACTAAGGATTATGATGTT+GGG - chr2_4:24765434-24765453 Msa0322340:intron 30.0%
TAAAGATGTTTGTGTTTGTG+TGG - chr2_4:24765525-24765544 Msa0322340:intron 30.0%
GATGATGATTGTTTACACTT+TGG - chr2_4:24765547-24765566 Msa0322340:intron 30.0%
ATTGTTTACACTTTGGTTGT+TGG - chr2_4:24765554-24765573 Msa0322340:intron 30.0%
CACCTTTCAAATTTCTGAAT+TGG - chr2_4:24765618-24765637 Msa0322340:intron 30.0%
ACTAGACATTCTTGACAAAA+TGG - chr2_4:24765936-24765955 Msa0322340:intron 30.0%
CTTTCTATAGCAAACATTTG+TGG - chr2_4:24765982-24766001 Msa0322340:intron 30.0%
ATATACAAACGCAAACAACT+AGG - chr2_4:24766114-24766133 Msa0322340:CDS 30.0%
! ATCTAAAGCTCTATGTATTC+TGG + chr2_4:24766184-24766203 None:intergenic 30.0%
CAGATGATGTAGAATTAGTA+AGG - chr2_4:24766205-24766224 Msa0322340:CDS 30.0%
GAACATGTAAGAAAATACCT+CGG + chr2_4:24766674-24766693 None:intergenic 30.0%
!! ACGATTCATTGTTTTTCAGT+TGG - chr2_4:24766798-24766817 Msa0322340:CDS 30.0%
ATGGATATCAATCCAATTGA+TGG - chr2_4:24766861-24766880 Msa0322340:intron 30.0%
! GGCACCATTTAAAATAAAAG+AGG - chr2_4:24766944-24766963 Msa0322340:intron 30.0%
!! TAAAAGAGGAGCATCTAATT+CGG - chr2_4:24766958-24766977 Msa0322340:intron 30.0%
GCTACAAGAAGTTCTTAATA+AGG - chr2_4:24767515-24767534 Msa0322340:CDS 30.0%
! AAAGAGGAGTATGATAAATC+TGG - chr2_4:24767552-24767571 Msa0322340:CDS 30.0%
ATCATACTATTATTAGCCCT+TGG + chr2_4:24767615-24767634 None:intergenic 30.0%
GAAGTAGTGATAGCTGAATT+TGG + chr2_4:24765196-24765215 None:intergenic 35.0%
ATATTGTCTGATAGACGGAT+CGG + chr2_4:24765247-24765266 None:intergenic 35.0%
GTGACTAAGGATTATGATGT+TGG - chr2_4:24765433-24765452 Msa0322340:intron 35.0%
GTTCTGCGTTATTTGTATAG+TGG - chr2_4:24765475-24765494 Msa0322340:intron 35.0%
TGAAGCATGAAATCAACAAC+AGG + chr2_4:24765580-24765599 None:intergenic 35.0%
TGAATTGGTTGCTCTTTATC+AGG - chr2_4:24765633-24765652 Msa0322340:intron 35.0%
!! CCATTTTGCTTTATTGTACG+CGG - chr2_4:24765773-24765792 Msa0322340:CDS 35.0%
!!! TACCCTGTTTTTTACACAAG+AGG - chr2_4:24765799-24765818 Msa0322340:CDS 35.0%
AGCCTCTTGTGTAAAAAACA+GGG + chr2_4:24765804-24765823 None:intergenic 35.0%
!! CAGTTGATTTGATTACTTGC+AGG - chr2_4:24765906-24765925 Msa0322340:CDS 35.0%
!!! GTAAGGTTGCTTTTGAAAGA+AGG - chr2_4:24766222-24766241 Msa0322340:CDS 35.0%
TCGATTGTGTATCGAGATAT+TGG - chr2_4:24766551-24766570 Msa0322340:CDS 35.0%
! AGAAAATACCTCGGTTTTCT+AGG + chr2_4:24766665-24766684 None:intergenic 35.0%
!! AGCAAAACTTTTGTTTCCGA+TGG - chr2_4:24766824-24766843 Msa0322340:intron 35.0%
AATTCAGATGTGCCATCAAT+TGG + chr2_4:24766876-24766895 None:intergenic 35.0%
GAATTTACTACGAATTTGGG+TGG - chr2_4:24766891-24766910 Msa0322340:intron 35.0%
AGTACTTTCTTAAAGGTCGA+AGG - chr2_4:24767042-24767061 Msa0322340:intron 35.0%
TCTTAATAAGGTCTTCCATG+AGG - chr2_4:24767527-24767546 Msa0322340:CDS 35.0%
AACGAAATCAGCAACAACAC+TGG - chr2_4:24765139-24765158 Msa0322340:CDS 40.0%
TCTGGCAATGATCTTTGCAT+CGG + chr2_4:24765308-24765327 None:intergenic 40.0%
TGCGAGGAGTAGTTTCTTTA+AGG - chr2_4:24765366-24765385 Msa0322340:intron 40.0%
CCGCGTACAATAAAGCAAAA+TGG + chr2_4:24765776-24765795 None:intergenic 40.0%
CAGCCTCTTGTGTAAAAAAC+AGG + chr2_4:24765805-24765824 None:intergenic 40.0%
GGCAATAGATGACATGTTGA+CGG - chr2_4:24765957-24765976 Msa0322340:intron 40.0%
AAGTCCAAGATCAAGAAGCT+CGG + chr2_4:24766316-24766335 None:intergenic 40.0%
! CCATCTAAAGTGAGTTCAGA+AGG + chr2_4:24766444-24766463 None:intergenic 40.0%
CCTTCTGAACTCACTTTAGA+TGG - chr2_4:24766441-24766460 Msa0322340:CDS 40.0%
AGATATGCCTCACCATGTAA+AGG + chr2_4:24766597-24766616 None:intergenic 40.0%
GGCATATCTTTCTCTTGCTA+AGG - chr2_4:24766608-24766627 Msa0322340:CDS 40.0%
TATCTTTCTCTTGCTAAGGC+AGG - chr2_4:24766612-24766631 Msa0322340:CDS 40.0%
ATCTTTCTCTTGCTAAGGCA+GGG - chr2_4:24766613-24766632 Msa0322340:CDS 40.0%
TTGTTGTACCTAGAAAACCG+AGG - chr2_4:24766654-24766673 Msa0322340:CDS 40.0%
TTCGGGAGTAACATATGTCA+AGG + chr2_4:24766753-24766772 None:intergenic 40.0%
TTTGTTTCCGATGGAAGCAA+AGG - chr2_4:24766833-24766852 Msa0322340:intron 40.0%
ATCACAACCTTTGCTTCCAT+CGG + chr2_4:24766843-24766862 None:intergenic 40.0%
GACAACTATGGACTTGAATG+AGG - chr2_4:24766923-24766942 Msa0322340:intron 40.0%
!! TTTTTGGTCATCATGAGCAG+TGG - chr2_4:24767356-24767375 Msa0322340:CDS 40.0%
!! GGAAGTGTTGAACAAGATCA+TGG - chr2_4:24767410-24767429 Msa0322340:CDS 40.0%
GCAAAGATCATTGCCAGAGA+TGG - chr2_4:24765310-24765329 Msa0322340:CDS 45.0%
AGAAACTTCTCGACCATCTC+TGG + chr2_4:24765326-24765345 None:intergenic 45.0%
AGAAACGATGCTGCAACTGA+AGG - chr2_4:24765408-24765427 Msa0322340:intron 45.0%
GCAACTGAAGGAAGTGACTA+AGG - chr2_4:24765420-24765439 Msa0322340:intron 45.0%
AGGCAGTGATTTCTCAAGAG+TGG + chr2_4:24766073-24766092 None:intergenic 45.0%
AAATCACTGCCTCAGCCTAT+AGG - chr2_4:24766081-24766100 Msa0322340:CDS 45.0%
AATCACTGCCTCAGCCTATA+GGG - chr2_4:24766082-24766101 Msa0322340:CDS 45.0%
ACTTGTTTCCCTATAGGCTG+AGG + chr2_4:24766093-24766112 None:intergenic 45.0%
AGGACACACTACTCTAGATG+AGG - chr2_4:24766242-24766261 Msa0322340:CDS 45.0%
AAGATCAAGAAGCTCGGTAG+TGG + chr2_4:24766310-24766329 None:intergenic 45.0%
ACTACCGAGCTTCTTGATCT+TGG - chr2_4:24766309-24766328 Msa0322340:CDS 45.0%
AAAGCGTAGTACGAAAGCTG+TGG - chr2_4:24766485-24766504 Msa0322340:CDS 45.0%
TATTGGAGCAAGCTGAGAGA+AGG - chr2_4:24766568-24766587 Msa0322340:CDS 45.0%
ATTGGAGCAAGCTGAGAGAA+GGG - chr2_4:24766569-24766588 Msa0322340:CDS 45.0%
TCTTTCTCTTGCTAAGGCAG+GGG - chr2_4:24766614-24766633 Msa0322340:CDS 45.0%
GATGGAAGCAAAGGTTGTGA+TGG - chr2_4:24766842-24766861 Msa0322340:intron 45.0%
TGGTTACCAGAGGACAACTA+TGG - chr2_4:24766911-24766930 Msa0322340:intron 45.0%
TCAAGTCCATAGTTGTCCTC+TGG + chr2_4:24766920-24766939 None:intergenic 45.0%
! GGAGCATCTAATTCGGATGA+AGG - chr2_4:24766965-24766984 Msa0322340:intron 45.0%
TCATCATGAGCAGTGGAGTT+GGG - chr2_4:24767363-24767382 Msa0322340:CDS 45.0%
CATACTCCTCTTTGTCCTCA+TGG + chr2_4:24767545-24767564 None:intergenic 45.0%
TCATCCTCATCCACGTCAAT+AGG - chr2_4:24767585-24767604 Msa0322340:CDS 45.0%
CATTCCTATTGACGTGGATG+AGG + chr2_4:24767592-24767611 None:intergenic 45.0%
CACGTCAATAGGAATGCCAA+GGG - chr2_4:24767596-24767615 Msa0322340:CDS 45.0%
!!! TATATTTTATTTTGATTTTT+TGG - chr2_4:24767340-24767359 Msa0322340:CDS 5.0%
TTGTCTGATAGACGGATCGG+TGG + chr2_4:24765244-24765263 None:intergenic 50.0%
GTCGAGAAGTTTCTGTGCAC+CGG - chr2_4:24765332-24765351 Msa0322340:CDS 50.0%
TCCTCGCAGACAAAATGCAC+CGG + chr2_4:24765354-24765373 None:intergenic 50.0%
!!! CTGCCTAGTTTTAGTGCACG+GGG + chr2_4:24765734-24765753 None:intergenic 50.0%
!!! CCTGCCTAGTTTTAGTGCAC+GGG + chr2_4:24765735-24765754 None:intergenic 50.0%
!!! CCCTGCCTAGTTTTAGTGCA+CGG + chr2_4:24765736-24765755 None:intergenic 50.0%
CCGTGCACTAAAACTAGGCA+GGG - chr2_4:24765733-24765752 Msa0322340:intron 50.0%
AAAATGGTGAGACCCCTTGC+AGG + chr2_4:24765760-24765779 None:intergenic 50.0%
GAGAGAAGGGAGCCTTTACA+TGG - chr2_4:24766582-24766601 Msa0322340:CDS 50.0%
AAGGGAGCCTTTACATGGTG+AGG - chr2_4:24766587-24766606 Msa0322340:CDS 50.0%
CGAATTTGGGTGGTTACCAG+AGG - chr2_4:24766901-24766920 Msa0322340:intron 50.0%
!! ATGAAGGCGTTGTCTAGAGC+AGG - chr2_4:24766981-24767000 Msa0322340:intron 50.0%
GTCATCATGAGCAGTGGAGT+TGG - chr2_4:24767362-24767381 Msa0322340:CDS 50.0%
CATCATGAGCAGTGGAGTTG+GGG - chr2_4:24767364-24767383 Msa0322340:CDS 50.0%
TCAACACTTCCGAGCAACGA+GGG + chr2_4:24767401-24767420 None:intergenic 50.0%
TTCAACACTTCCGAGCAACG+AGG + chr2_4:24767402-24767421 None:intergenic 50.0%
TGCAAGCTGCGATAGGTCAT+CGG + chr2_4:24767439-24767458 None:intergenic 50.0%
CTATCGCAGCTTGCATGCAT+GGG - chr2_4:24767444-24767463 Msa0322340:CDS 50.0%
CGCTTCACTTGTCGATCTTC+GGG + chr2_4:24767480-24767499 None:intergenic 50.0%
GCGCTTCACTTGTCGATCTT+CGG + chr2_4:24767481-24767500 None:intergenic 50.0%
GGTCTTCCATGAGGACAAAG+AGG - chr2_4:24767536-24767555 Msa0322340:CDS 50.0%
CCTTGGCATTCCTATTGACG+TGG + chr2_4:24767598-24767617 None:intergenic 50.0%
CCACGTCAATAGGAATGCCA+AGG - chr2_4:24767595-24767614 Msa0322340:CDS 50.0%
! ACCGGTGCATTTTGTCTGCG+AGG - chr2_4:24765350-24765369 Msa0322340:CDS 55.0%
CAGCCCCGTGCACTAAAACT+AGG - chr2_4:24765728-24765747 Msa0322340:intron 55.0%
CCCGTGCACTAAAACTAGGC+AGG - chr2_4:24765732-24765751 Msa0322340:intron 55.0%
AAACTAGGCAGGGTCCTGCA+AGG - chr2_4:24765743-24765762 Msa0322340:intron 55.0%
AACTAGGCAGGGTCCTGCAA+GGG - chr2_4:24765744-24765763 Msa0322340:intron 55.0%
GAGCAGTGGAGTTGGGGAAA+CGG - chr2_4:24767370-24767389 Msa0322340:CDS 55.0%
ACGGTTCTTCCCTCGTTGCT+CGG - chr2_4:24767389-24767408 Msa0322340:CDS 55.0%
CCATGCATGCAAGCTGCGAT+AGG + chr2_4:24767446-24767465 None:intergenic 55.0%
CCTATCGCAGCTTGCATGCA+TGG - chr2_4:24767443-24767462 Msa0322340:CDS 55.0%
AGTGAAGCGCAGACGATACG+CGG - chr2_4:24767491-24767510 Msa0322340:CDS 55.0%
ACTAGGCAGGGTCCTGCAAG+GGG - chr2_4:24765745-24765764 Msa0322340:intron 60.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 24765103 24767650 24765103 ID=Msa0322340;Name=Msa0322340
chr2_4 mRNA 24765103 24767650 24765103 ID=Msa0322340-mRNA-1;Parent=Msa0322340;Name=Msa0322340-mRNA-1;_AED=0.09;_eAED=0.09;_QI=0|0|0|1|1|1|4|0|586
chr2_4 exon 24767099 24767650 24767099 ID=Msa0322340-mRNA-1:exon:8856;Parent=Msa0322340-mRNA-1
chr2_4 exon 24766078 24766825 24766078 ID=Msa0322340-mRNA-1:exon:8855;Parent=Msa0322340-mRNA-1
chr2_4 exon 24765751 24765936 24765751 ID=Msa0322340-mRNA-1:exon:8854;Parent=Msa0322340-mRNA-1
chr2_4 exon 24765103 24765377 24765103 ID=Msa0322340-mRNA-1:exon:8853;Parent=Msa0322340-mRNA-1
chr2_4 CDS 24767099 24767650 24767099 ID=Msa0322340-mRNA-1:cds;Parent=Msa0322340-mRNA-1
chr2_4 CDS 24766078 24766825 24766078 ID=Msa0322340-mRNA-1:cds;Parent=Msa0322340-mRNA-1
chr2_4 CDS 24765751 24765936 24765751 ID=Msa0322340-mRNA-1:cds;Parent=Msa0322340-mRNA-1
chr2_4 CDS 24765103 24765377 24765103 ID=Msa0322340-mRNA-1:cds;Parent=Msa0322340-mRNA-1
Gene Sequence

>Msa0322340

ATGATGTACTTGAGAAGTGGATTCTCTGAATATTCAAACGAAATCAGCAACAACACTGGCAGTGCTAGTGAGTTATGCTGCACAACAGCACCAAATTCAGCTATCACTACTTCACAAGATATCATCACAATCACACAACCACCGATCCGTCTATCAGACAATATAGCTTCAATACTAAATGATACAGCGTTTGACTTCTTTTCCGATGCAAAGATCATTGCCAGAGATGGTCGAGAAGTTTCTGTGCACCGGTGCATTTTGTCTGCGAGGAGTAGTTTCTTTAAGGATGTTTTTAAGGGAAAGAAAGAAACGATGCTGCAACTGAAGGAAGTGACTAAGGATTATGATGTTGGGTTTGACGCGCTTAACGTTGTTCTGCGTTATTTGTATAGTGGCAGAGTTGATCATCATCATTTGTCTGCTAAAGATGTTTGTGTTTGTGTGGATGATGATTGTTTACACTTTGGTTGTTGGCCTGTTGTTGATTTCATGCTTCAACTTCTCTATGCTTCATTCACCTTTCAAATTTCTGAATTGGTTGCTCTTTATCAGGATCATCTACTAGACATTCTTGACAAAATGGCAATAGATGACATGTTGACGGTTCTTTCTATAGCAAACATTTGTGGTCAAACATGTGACAAACTACTAACAAGATGTACAGATATAACAGTTGAATCTAATGTTGATATAACCACTCTTGAGAAATCACTGCCTCAGCCTATAGGGAAACAAGTAATATACAAACGCAAACAACTAGGTTTAGACATGTATGAAACCGTAAATCTTCTAGACAAACATGTCACCAGAATACATAGAGCTTTAGATTCAGATGATGTAGAATTAGTAAGGTTGCTTTTGAAAGAAGGACACACTACTCTAGATGAGGCTCATGCACTTCACTATGCTGTTGCATATTGTGATGTCAAAACCACTACCGAGCTTCTTGATCTTGGACTTGCCGATGTAAATCATAAAAATCTTAGGGGCTATAGCGTGCTACATGTTGCTGCGAAGAGAAAAGAACCGAAAATTATTGTATCTTTGTTAACGAAGGGAGCTCAACCTTCTGAACTCACTTTAGATGGCAGAAAAGCTCTTCAAATTTCAAAGCGTAGTACGAAAGCTGTGGATTATTATAAATCTACTGAGGAAGGAAAAGTTTCTTCGAATGATCGATTGTGTATCGAGATATTGGAGCAAGCTGAGAGAAGGGAGCCTTTACATGGTGAGGCATATCTTTCTCTTGCTAAGGCAGGGGATGATTTACGTATGAAATTGTTGTACCTAGAAAACCGAGTTGGACTAGCAAAACTTTTGTTTCCGATGGAAGCAAAGGTTGTGATGGATATCAATCCAATTGATGGCACATCTGAATTTACTACGAATTTGGGTGGTTACCAGAGGACAACTATGGACTTGAATGAGGCACCATTTAAAATAAAAGAGGAGCATCTAATTCGGATGAAGGCGTTGTCTAGAGCAGTGGAGTTGGGGAAACGGTTCTTCCCTCGTTGCTCGGAAGTGTTGAACAAGATCATGGATGCCGATGACCTATCGCAGCTTGCATGCATGGGTCATGACAGTCCCGAAGATCGACAAGTGAAGCGCAGACGATACGCGGAGCTACAAGAAGTTCTTAATAAGGTCTTCCATGAGGACAAAGAGGAGTATGATAAATCTGGCATGTCTTCATCATCCTCATCCACGTCAATAGGAATGCCAAGGGCTAATAATAGTATGATAGCAATGAATCATTGA
Protein sequence

>Msa0322340

MMYLRSGFSEYSNEISNNTGSASELCCTTAPNSAITTSQDIITITQPPIRLSDNIASILNDTAFDFFSDAKIIARDGREVSVHRCILSARSSFFKDVFKGKKETMLQLKEVTKDYDVGFDALNVVLRYLYSGRVDHHHLSAKDVCVCVDDDCLHFGCWPVVDFMLQLLYASFTFQISELVALYQDHLLDILDKMAIDDMLTVLSIANICGQTCDKLLTRCTDITVESNVDITTLEKSLPQPIGKQVIYKRKQLGLDMYETVNLLDKHVTRIHRALDSDDVELVRLLLKEGHTTLDEAHALHYAVAYCDVKTTTELLDLGLADVNHKNLRGYSVLHVAAKRKEPKIIVSLLTKGAQPSELTLDGRKALQISKRSTKAVDYYKSTEEGKVSSNDRLCIEILEQAERREPLHGEAYLSLAKAGDDLRMKLLYLENRVGLAKLLFPMEAKVVMDINPIDGTSEFTTNLGGYQRTTMDLNEAPFKIKEEHLIRMKALSRAVELGKRFFPRCSEVLNKIMDADDLSQLACMGHDSPEDRQVKRRRYAELQEVLNKVFHEDKEEYDKSGMSSSSSSTSIGMPRANNSMIAMNH*