AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0323240

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0323240 MtrunA17_Chr2g0277461 88.486 469 31 4 1 464 1 451 0.0 836
Msa0323240 MtrunA17_Chr2g0277531 72.152 316 72 6 7 317 14 318 1.95e-160 456
Msa0323240 MtrunA17_Chr5g0424931 45.541 527 209 13 7 466 13 528 3.17e-135 400
Msa0323240 MtrunA17_Chr1g0178011 49.891 459 198 10 6 455 5 440 3.82e-134 394
Msa0323240 MtrunA17_Chr5g0417281 43.726 526 224 13 3 466 1 516 4.78e-121 363
Msa0323240 MtrunA17_Chr5g0436761 43.376 468 239 13 5 459 4 458 1.36e-107 327
Msa0323240 MtrunA17_Chr5g0417211 42.857 469 194 12 5 465 4 406 1.81e-105 320
Msa0323240 MtrunA17_Chr2g0289221 52.516 318 136 6 6 317 13 321 1.72e-102 310
Msa0323240 MtrunA17_Chr3g0115271 35.663 558 252 16 5 465 11 558 9.75e-95 298
Msa0323240 MtrunA17_Chr5g0424801 45.042 353 164 13 7 344 6 343 4.11e-89 276
Msa0323240 MtrunA17_Chr5g0425261 45.198 354 164 13 7 346 6 343 4.38e-89 276
Msa0323240 MtrunA17_Chr5g0424741 41.388 418 176 15 6 363 5 413 1.10e-80 259
Msa0323240 MtrunA17_Chr2g0326761 39.152 401 179 12 12 358 10 399 6.53e-80 261
Msa0323240 MtrunA17_Chr4g0028971 36.740 411 191 11 4 355 3 403 4.10e-76 251
Msa0323240 MtrunA17_Chr1g0181211 35.075 402 191 11 12 353 26 417 5.67e-74 246
Msa0323240 MtrunA17_Chr1g0199681 38.035 397 186 13 13 358 94 481 2.97e-73 244
Msa0323240 MtrunA17_Chr7g0264171 35.507 414 191 15 10 358 28 430 2.98e-68 229
Msa0323240 MtrunA17_Chr7g0264161 39.617 313 158 12 23 317 2 301 2.26e-59 197
Msa0323240 MtrunA17_Chr5g0425661 49.268 205 93 4 13 214 37 233 3.28e-56 186
Msa0323240 MtrunA17_Chr8g0385791 32.398 392 247 9 13 391 59 445 7.17e-51 184
Msa0323240 MtrunA17_Chr8g0376771 33.429 350 217 8 13 349 23 369 2.87e-48 177
Msa0323240 MtrunA17_Chr4g0070181 33.333 354 209 10 10 348 20 361 3.02e-48 178
Msa0323240 MtrunA17_Chr2g0282961 30.747 348 205 9 13 337 53 387 1.61e-47 173
Msa0323240 MtrunA17_Chr2g0285461 32.670 352 218 8 13 349 23 370 2.33e-47 175
Msa0323240 MtrunA17_Chr2g0301251 32.768 354 211 10 10 348 20 361 3.33e-47 175
Msa0323240 MtrunA17_Chr3g0109391 31.842 380 239 8 13 379 26 398 4.27e-47 174
Msa0323240 MtrunA17_Chr5g0433301 32.000 350 223 7 13 349 24 371 4.79e-46 171
Msa0323240 MtrunA17_Chr2g0276781 32.500 360 218 9 13 356 23 373 2.82e-45 167
Msa0323240 MtrunA17_Chr1g0156321 33.529 340 207 9 13 338 43 377 3.33e-45 169
Msa0323240 MtrunA17_Chr4g0046371 32.044 362 196 11 8 337 33 376 5.13e-44 164
Msa0323240 MtrunA17_Chr8g0389081 32.479 351 217 10 5 340 24 369 3.03e-43 163
Msa0323240 MtrunA17_Chr4g0009751 30.055 366 237 9 13 365 19 378 1.28e-41 156
Msa0323240 MtrunA17_Chr8g0346711 30.814 344 217 9 13 343 29 364 9.05e-40 152
Msa0323240 MtrunA17_Chr7g0239061 28.647 377 243 9 13 373 14 380 1.12e-39 151
Msa0323240 MtrunA17_Chr4g0029671 30.435 368 228 10 13 356 54 417 1.01e-37 146
Msa0323240 MtrunA17_Chr2g0326281 30.278 360 212 8 13 337 56 411 1.18e-37 146
Msa0323240 MtrunA17_Chr5g0419861 58.333 120 40 6 51 165 9 123 6.64e-33 121
Msa0323240 MtrunA17_Chr5g0425651 48.780 123 51 6 239 350 1 122 1.57e-24 98.2
Msa0323240 MtrunA17_Chr5g0424791 41.830 153 71 5 66 214 13 151 1.27e-23 96.7
Msa0323240 MtrunA17_Chr8g0356211 27.928 222 136 8 131 337 8 220 4.73e-17 82.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0323240 AT1G77850.1 37.653 409 198 11 9 370 15 413 3.10e-85 273
Msa0323240 AT1G77850.2 37.653 409 198 11 9 370 15 413 4.90e-85 273
Msa0323240 AT4G30080.1 37.157 401 187 13 12 358 18 407 1.24e-73 244
Msa0323240 AT2G28350.2 35.749 414 195 12 5 358 2 404 2.63e-72 241
Msa0323240 AT2G28350.1 35.749 414 195 12 5 358 2 404 2.73e-72 241
Msa0323240 AT4G23980.2 33.731 335 208 6 13 337 12 342 1.35e-49 179
Msa0323240 AT4G23980.1 33.731 335 208 6 13 337 12 342 1.42e-49 179
Msa0323240 AT5G37020.2 31.806 371 238 7 13 370 22 390 2.34e-48 177
Msa0323240 AT5G37020.1 31.806 371 238 7 13 370 22 390 2.77e-48 177
Msa0323240 AT1G30330.3 33.428 353 214 9 13 349 25 372 3.59e-47 175
Msa0323240 AT1G30330.2 33.428 353 214 9 13 349 25 372 3.59e-47 175
Msa0323240 AT1G30330.1 33.428 353 214 9 13 349 23 370 3.71e-47 175
Msa0323240 AT5G62000.4 32.099 405 255 9 13 403 61 459 5.93e-47 174
Msa0323240 AT5G62000.2 32.099 405 255 9 13 403 61 459 6.63e-47 174
Msa0323240 AT5G62000.5 32.099 405 255 9 13 403 61 459 6.63e-47 174
Msa0323240 AT5G62000.3 32.099 405 255 9 13 403 61 459 6.63e-47 174
Msa0323240 AT5G62000.1 32.099 405 255 9 13 403 61 459 6.63e-47 174
Msa0323240 AT5G20730.1 32.687 361 217 9 3 348 14 363 8.07e-47 174
Msa0323240 AT5G20730.3 32.687 361 217 9 3 348 14 363 1.05e-46 174
Msa0323240 AT2G33860.1 31.988 347 202 9 13 337 54 388 1.55e-46 170
Msa0323240 AT2G46530.1 31.421 366 229 9 13 364 21 378 2.31e-46 170
Msa0323240 AT5G20730.2 32.687 361 216 10 3 348 14 362 3.13e-46 172
Msa0323240 AT2G46530.3 31.421 366 229 9 13 364 42 399 3.78e-46 169
Msa0323240 AT1G34310.1 33.237 346 200 9 8 337 22 352 4.94e-45 166
Msa0323240 AT3G61830.1 31.680 363 218 10 13 356 25 376 5.05e-45 166
Msa0323240 AT1G19850.2 34.294 347 202 11 10 338 40 378 2.35e-44 167
Msa0323240 AT1G19850.1 34.294 347 202 11 10 338 51 389 2.42e-44 167
Msa0323240 AT1G34390.1 32.659 346 200 10 8 337 22 350 8.59e-44 162
Msa0323240 AT1G35520.1 32.174 345 215 8 3 337 17 352 1.10e-43 162
Msa0323240 AT1G19220.1 30.833 360 220 10 4 348 16 361 2.14e-43 164
Msa0323240 AT5G60450.2 31.445 353 212 8 8 337 36 381 2.24e-43 163
Msa0323240 AT5G60450.1 31.609 348 208 8 13 337 65 405 5.93e-43 162
Msa0323240 AT1G35540.1 32.456 342 208 10 8 337 22 352 2.67e-42 159
Msa0323240 AT1G35240.1 32.353 340 211 8 8 337 22 352 3.16e-42 158
Msa0323240 AT1G34410.1 31.765 340 213 8 8 337 22 352 1.55e-41 156
Msa0323240 AT1G59750.2 31.339 351 222 9 13 350 22 366 1.72e-40 154
Msa0323240 AT1G59750.1 31.339 351 222 9 13 350 22 366 2.29e-40 154
Msa0323240 AT1G59750.3 31.339 351 222 9 13 350 22 366 2.29e-40 154
Msa0323240 AT1G59750.4 31.339 351 222 9 13 350 22 366 2.51e-40 154
Msa0323240 AT1G34170.1 30.372 349 222 9 1 337 13 352 5.06e-40 150
Msa0323240 AT1G34170.2 29.942 344 204 8 1 337 13 326 1.15e-37 144
Msa0323240 AT1G34170.3 29.942 344 204 8 1 337 13 326 4.18e-37 143
Msa0323240 AT3G61830.2 31.392 309 182 10 67 356 28 325 6.39e-32 128
Msa0323240 AT5G37020.3 32.584 267 165 7 117 370 31 295 3.03e-31 127
Msa0323240 AT1G43950.1 34.259 216 121 5 8 217 22 222 5.81e-30 116
Msa0323240 AT2G33860.2 32.479 234 141 6 117 337 2 231 6.03e-30 121
Msa0323240 AT2G46530.2 31.679 262 164 7 114 364 34 291 3.50e-29 120
Msa0323240 ATMG00940.1 43.210 81 42 3 236 313 65 144 9.44e-11 60.8

Find 101 sgRNAs with CRISPR-Local

Find 182 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATCTCGTACTTCTAATTTA+TGG 0.108193 2_4:-26918342 Msa0323240:CDS
TTTGAATATCGTAGGTTAAT+TGG 0.128409 2_4:-26916953 Msa0323240:intron
GATGGTTCAATTATGTTATT+TGG 0.174464 2_4:-26916594 Msa0323240:CDS
GCACATGTGTGCCAAATTTC+TGG 0.215696 2_4:+26919222 None:intergenic
CGCATGTGCGTCCAGAAATT+TGG 0.217939 2_4:-26919233 Msa0323240:CDS
TTCATCTTAACTCTCATTCT+AGG 0.234993 2_4:+26918412 None:intergenic
GATTGGCAACCTCTTCTTTA+GGG 0.275578 2_4:+26918956 None:intergenic
AGATTGGCAACCTCTTCTTT+AGG 0.275924 2_4:+26918955 None:intergenic
TAATTTATGGCGCATGCTTC+AGG 0.288610 2_4:-26918329 Msa0323240:intron
ATAGAGATTGTCCCTTGATA+TGG 0.294890 2_4:+26918367 None:intergenic
AGATGGACATCGTTGGTTAT+TGG 0.309930 2_4:+26918985 None:intergenic
TTGAATATCGTAGGTTAATT+GGG 0.314702 2_4:-26916952 Msa0323240:intron
TGCCCAACTAAGTGATAAAA+AGG 0.319098 2_4:-26916808 Msa0323240:CDS
CTGTTCGTTACCGACGTTCA+TGG 0.320691 2_4:-26918787 Msa0323240:CDS
TTCATCGCGGTTGTTGAGAT+TGG 0.361556 2_4:+26918939 None:intergenic
TAAACTCTTGAATGCAACTT+TGG 0.370692 2_4:+26919180 None:intergenic
GATATATAACATGTTTCTCT+TGG 0.384809 2_4:+26918726 None:intergenic
CAGAGGAGTTCAAAATAATA+TGG 0.402834 2_4:+26916664 None:intergenic
AATGGAATTCCTTTGTTAAG+AGG 0.404581 2_4:-26918699 Msa0323240:CDS
TCAACGGGAAAGTTATTTGT+TGG 0.427846 2_4:-26918634 Msa0323240:CDS
CAACGGGAAAGTTATTTGTT+GGG 0.431447 2_4:-26918633 Msa0323240:CDS
GCAGTTTGTTGCAGCGGCGG+CGG 0.440577 2_4:-26918596 Msa0323240:CDS
TGAGTTGGAGAAGGCAGTAA+TGG 0.441158 2_4:-26918560 Msa0323240:CDS
TCATCTTAACTCTCATTCTA+GGG 0.445548 2_4:+26918413 None:intergenic
ATGAATCAATGAAGATTCAA+TGG 0.450458 2_4:-26918438 Msa0323240:CDS
TGAGTCTTTCATGAAAATAA+CGG 0.452973 2_4:+26918654 None:intergenic
TGAAGACTGATAAATCTTCA+AGG 0.454632 2_4:-26918393 Msa0323240:CDS
CAGGTGTTGAGACGAGGATT+CGG 0.457588 2_4:+26918807 None:intergenic
TTGGGATGAATTTCAAGTCT+CGG 0.458166 2_4:-26916934 Msa0323240:CDS
ACTATCCTCGAGGGGATGAT+TGG 0.466368 2_4:-26918492 Msa0323240:CDS
AGTCAACCCGTGGTGGGTTG+AGG 0.468552 2_4:-26916898 Msa0323240:CDS
CACGCAGTTTGTTGCAGCGG+CGG 0.472211 2_4:-26918599 Msa0323240:CDS
CATGAACGTCGGTAACGAAC+AGG 0.475779 2_4:+26918788 None:intergenic
ATATATAACATGTTTCTCTT+GGG 0.481990 2_4:+26918727 None:intergenic
TAGAGATTGTCCCTTGATAT+GGG 0.482488 2_4:+26918368 None:intergenic
AAAAGAGTGTGTGTGGGTAA+TGG 0.489755 2_4:+26919107 None:intergenic
TCTCATATCAAGGGAGATGA+TGG 0.490070 2_4:-26916534 Msa0323240:CDS
CACCACGGGTTGACTCGTCT+TGG 0.505317 2_4:+26916906 None:intergenic
TGCCGATCCTCACACGGATG+AGG 0.509768 2_4:-26919031 Msa0323240:CDS
TCTTCAAGGATCCCATATCA+AGG 0.512818 2_4:-26918379 Msa0323240:CDS
AGTTATTTGTTGGGATTCGC+AGG 0.513561 2_4:-26918624 Msa0323240:CDS
AAGCCGCGGGAAGACATTAT+CGG 0.516617 2_4:+26918840 None:intergenic
AGTAATGGAGGCATTGAAGT+TGG 0.517015 2_4:-26918545 Msa0323240:CDS
GACCTCATCCGTGTGAGGAT+CGG 0.528604 2_4:+26919029 None:intergenic
TTTCACAATTCTCATATCAA+GGG 0.529587 2_4:-26916543 Msa0323240:CDS
ACATGTTATATATCTCTGAA+TGG 0.530162 2_4:-26918717 Msa0323240:CDS
AGCGGCGGCGGAGCAGAAGA+AGG 0.532222 2_4:-26918584 Msa0323240:CDS
AAGAGGAAGAAACTCGTGGC+TGG 0.538632 2_4:-26918682 Msa0323240:CDS
GGAGCAGAAGAAGGATGAGT+TGG 0.543711 2_4:-26918575 Msa0323240:CDS
GGTGATACTTTATCATGTAT+AGG 0.550061 2_4:+26916693 None:intergenic
TGGGAAACCCGCGGCAGACA+TGG 0.563883 2_4:+26918746 None:intergenic
AAATTGTTTACTATCCTCGA+GGG 0.568194 2_4:-26918501 Msa0323240:CDS
CGGCACAAAAGCGAGGTATG+TGG 0.571206 2_4:+26918860 None:intergenic
GAAGAAGGATGAGTTGGAGA+AGG 0.573545 2_4:-26918569 Msa0323240:CDS
AGAGTGTGTGTGGGTAATGG+AGG 0.575190 2_4:+26919110 None:intergenic
GAGACGAGGATTCGGCCTCT+AGG 0.578409 2_4:+26918815 None:intergenic
ATTTCTGGACGCACATGCGA+TGG 0.580120 2_4:+26919237 None:intergenic
TCTTGAAAACCCTAAAGAAG+AGG 0.581476 2_4:-26918965 Msa0323240:CDS
TCTCCTTGCCGATCCTCACA+CGG 0.588085 2_4:-26919037 Msa0323240:CDS
CTTCCCGCGGCTTGACCTAG+AGG 0.588102 2_4:-26918830 Msa0323240:CDS
AATGGAGGCATTGAAGTTGG+CGG 0.590740 2_4:-26918542 Msa0323240:CDS
AATGAGATCACTTCAACGGA+AGG 0.592309 2_4:-26916750 Msa0323240:CDS
GAAGTTGGCGGAAGAGAACA+AGG 0.600106 2_4:-26918530 Msa0323240:CDS
GTTCTGATGTTAACAATGCT+CGG 0.602948 2_4:-26918888 Msa0323240:CDS
AAATAACCTCAACCCACCAC+GGG 0.608942 2_4:+26916892 None:intergenic
CTTCAAGGATCCCATATCAA+GGG 0.609531 2_4:-26918378 Msa0323240:CDS
GAGACGATACAAGGAGTGAA+AGG 0.609567 2_4:+26919069 None:intergenic
TTTGTAGAAAAGAGTGTGTG+TGG 0.609647 2_4:+26919100 None:intergenic
GAACACGCAGTTTGTTGCAG+CGG 0.616426 2_4:-26918602 Msa0323240:CDS
GAGTTTCTTCCTCTTAACAA+AGG 0.617502 2_4:+26918690 None:intergenic
TTTGAATGAGATCACTTCAA+CGG 0.618869 2_4:-26916754 Msa0323240:CDS
GTGTTGCAATTGTGAGTCGA+AGG 0.619072 2_4:+26916630 None:intergenic
CGGCCTCTAGGTCAAGCCGC+GGG 0.620797 2_4:+26918827 None:intergenic
CGAAGGAAACTTTGTCTCAG+AGG 0.621557 2_4:+26916647 None:intergenic
TCAACAGCTGAGACGATACA+AGG 0.622390 2_4:+26919060 None:intergenic
GAAATTGTTTACTATCCTCG+AGG 0.624214 2_4:-26918502 Msa0323240:CDS
GTTGGAGAAGGCAGTAATGG+AGG 0.624776 2_4:-26918557 Msa0323240:CDS
ATGAGATCACTTCAACGGAA+GGG 0.630141 2_4:-26916749 Msa0323240:CDS
GTAATGGAGGAAGAAGAAGA+AGG 0.639327 2_4:+26919123 None:intergenic
AGAGTTTATTATTTCCCACA+AGG 0.642714 2_4:-26919165 Msa0323240:CDS
AACGAACAGGTGTTGAGACG+AGG 0.644655 2_4:+26918801 None:intergenic
CAAGACGAGTCAACCCGTGG+TGG 0.644679 2_4:-26916905 Msa0323240:CDS
TTGTGAAAATTGCTTACATG+AGG 0.646619 2_4:+26916558 None:intergenic
AAGACGAGTCAACCCGTGGT+GGG 0.646955 2_4:-26916904 Msa0323240:CDS
TCGGCCTCTAGGTCAAGCCG+CGG 0.648171 2_4:+26918826 None:intergenic
GAAATAACCTCAACCCACCA+CGG 0.649182 2_4:+26916891 None:intergenic
TGGTTGCTTCTGAACAGACA+TGG 0.659994 2_4:+26919257 None:intergenic
TGTTTCTCTTGGGAAACCCG+CGG 0.663878 2_4:+26918737 None:intergenic
ACAAAGACCTCATCCGTGTG+AGG 0.664295 2_4:+26919024 None:intergenic
TGTTAAGAGGAAGAAACTCG+TGG 0.667790 2_4:-26918686 Msa0323240:CDS
TTCCAAGACGAGTCAACCCG+TGG 0.669719 2_4:-26916908 Msa0323240:CDS
TTCAAAATAATATGGCTGTG+AGG 0.673161 2_4:+26916672 None:intergenic
ACATTATCGGCACAAAAGCG+AGG 0.676914 2_4:+26918853 None:intergenic
TTGTAGAAAAGAGTGTGTGT+GGG 0.681947 2_4:+26919101 None:intergenic
GTGCCGATAATGTCTTCCCG+CGG 0.682542 2_4:-26918843 Msa0323240:CDS
GATGATGGCTATAAGAGCTG+CGG 0.682568 2_4:-26916519 Msa0323240:CDS
TGAAACTTCACCATGAACGT+CGG 0.683050 2_4:+26918777 None:intergenic
AATTGTTTACTATCCTCGAG+GGG 0.689459 2_4:-26918500 Msa0323240:CDS
CATCCGTGTGAGGATCGGCA+AGG 0.710355 2_4:+26919034 None:intergenic
TCACACCAATCATCCCCTCG+AGG 0.748749 2_4:+26918487 None:intergenic
CAAAAGAAACAACTTCATCG+CGG 0.749390 2_4:+26918926 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GATTTTCTTAAATTTATTAT+CGG + chr2_4:26917579-26917598 None:intergenic 10.0%
!!! ATAATTTCTTTTTCTTTTCT+TGG - chr2_4:26917456-26917475 Msa0323240:intron 15.0%
!!! TTTTTTTGTGGTATTTTTTT+TGG - chr2_4:26917723-26917742 Msa0323240:intron 15.0%
!!! TATTTTTTTTGGTATAAATC+AGG - chr2_4:26917734-26917753 Msa0323240:intron 15.0%
!! CGTATATTTGAATATTTAAT+TGG - chr2_4:26918075-26918094 Msa0323240:intron 15.0%
!! TAGTTACTAACATTAAAAAA+AGG + chr2_4:26918122-26918141 None:intergenic 15.0%
!! GTAAAAAAAAATAGATGTTA+AGG + chr2_4:26918165-26918184 None:intergenic 15.0%
!! ACGATATTCAAATAAAAAAA+TGG + chr2_4:26918800-26918819 None:intergenic 15.0%
!!! TTTTTTTATTTGAATATCGT+AGG - chr2_4:26918800-26918819 Msa0323240:CDS 15.0%
!!! AGTTCTAAATTTTTTTGTTT+GGG + chr2_4:26918922-26918941 None:intergenic 15.0%
!!! TAGTTCTAAATTTTTTTGTT+TGG + chr2_4:26918923-26918942 None:intergenic 15.0%
!! ATATATAACATGTTTCTCTT+GGG + chr2_4:26917037-26917056 None:intergenic 20.0%
!!! GTTGATAAATGATTTTTTTG+TGG - chr2_4:26917711-26917730 Msa0323240:intron 20.0%
!! TCTAATTATAGATATAGCAT+TGG + chr2_4:26917808-26917827 None:intergenic 20.0%
!! TTTAACTATAGATGAAACTT+TGG + chr2_4:26918582-26918601 None:intergenic 20.0%
!! ATAGTTAAATGACTCATTAA+AGG - chr2_4:26918593-26918612 Msa0323240:CDS 20.0%
!!! AGACTTTATTTGAACAATAT+TGG - chr2_4:26918692-26918711 Msa0323240:CDS 20.0%
!!! TTCTAAATTTTTTTGTTTGG+GGG + chr2_4:26918920-26918939 None:intergenic 20.0%
!!! GTTCTAAATTTTTTTGTTTG+GGG + chr2_4:26918921-26918940 None:intergenic 20.0%
! GATATATAACATGTTTCTCT+TGG + chr2_4:26917038-26917057 None:intergenic 25.0%
! ACATGTTATATATCTCTGAA+TGG - chr2_4:26917044-26917063 Msa0323240:intron 25.0%
! TGAGTCTTTCATGAAAATAA+CGG + chr2_4:26917110-26917129 None:intergenic 25.0%
!! TATTTTCATGAAAGACTCAA+CGG - chr2_4:26917111-26917130 Msa0323240:intron 25.0%
! ATGAATCAATGAAGATTCAA+TGG - chr2_4:26917323-26917342 Msa0323240:intron 25.0%
! TATCTCGTACTTCTAATTTA+TGG - chr2_4:26917419-26917438 Msa0323240:intron 25.0%
! AAATGAAATGCAATATCGAT+TGG + chr2_4:26918019-26918038 None:intergenic 25.0%
!! TCGGTTAATTTACCAATTTT+GGG - chr2_4:26918465-26918484 Msa0323240:CDS 25.0%
!! GTCATATGAGATAGATTTTA+AGG - chr2_4:26918626-26918645 Msa0323240:CDS 25.0%
!!! ATGAGATAGATTTTAAGGTT+AGG - chr2_4:26918631-26918650 Msa0323240:CDS 25.0%
!!! TGAGATAGATTTTAAGGTTA+GGG - chr2_4:26918632-26918651 Msa0323240:CDS 25.0%
! TTTGAATATCGTAGGTTAAT+TGG - chr2_4:26918808-26918827 Msa0323240:CDS 25.0%
! TTGAATATCGTAGGTTAATT+GGG - chr2_4:26918809-26918828 Msa0323240:CDS 25.0%
!! TTCCTTTTTATCACTTAGTT+GGG + chr2_4:26918958-26918977 None:intergenic 25.0%
!! TTTCCTTTTTATCACTTAGT+TGG + chr2_4:26918959-26918978 None:intergenic 25.0%
! AACACCAAAAATTATAGTGA+TGG - chr2_4:26919149-26919168 Msa0323240:CDS 25.0%
!! TGAACCATCACTATAATTTT+TGG + chr2_4:26919156-26919175 None:intergenic 25.0%
! GATGGTTCAATTATGTTATT+TGG - chr2_4:26919167-26919186 Msa0323240:CDS 25.0%
!! TTTTCACAATTCTCATATCA+AGG - chr2_4:26919217-26919236 Msa0323240:CDS 25.0%
! TTTCACAATTCTCATATCAA+GGG - chr2_4:26919218-26919237 Msa0323240:CDS 25.0%
!!! ATGCAACTTTGGAATTTTGA+CGG + chr2_4:26916573-26916592 None:intergenic 30.0%
TAAACTCTTGAATGCAACTT+TGG + chr2_4:26916584-26916603 None:intergenic 30.0%
AGAGTTTATTATTTCCCACA+AGG - chr2_4:26916596-26916615 Msa0323240:CDS 30.0%
AATGGAATTCCTTTGTTAAG+AGG - chr2_4:26917062-26917081 Msa0323240:intron 30.0%
! ATTTTCATGAAAGACTCAAC+GGG - chr2_4:26917112-26917131 Msa0323240:intron 30.0%
AAATTGTTTACTATCCTCGA+GGG - chr2_4:26917260-26917279 Msa0323240:intron 30.0%
TCATCTTAACTCTCATTCTA+GGG + chr2_4:26917351-26917370 None:intergenic 30.0%
TTCATCTTAACTCTCATTCT+AGG + chr2_4:26917352-26917371 None:intergenic 30.0%
TGAAGACTGATAAATCTTCA+AGG - chr2_4:26917368-26917387 Msa0323240:intron 30.0%
GTGTTTCATAGCTAGATAAT+TGG + chr2_4:26917504-26917523 None:intergenic 30.0%
TATTAAACGTCTCCTAATGT+CGG + chr2_4:26917651-26917670 None:intergenic 30.0%
ATACTAGATACTATGAAGCA+CGG + chr2_4:26917682-26917701 None:intergenic 30.0%
AACATTAAAAAAAGGAGCGA+CGG + chr2_4:26918114-26918133 None:intergenic 30.0%
CTGTAATAATCTCTCTATGT+GGG + chr2_4:26918237-26918256 None:intergenic 30.0%
TCTGTAATAATCTCTCTATG+TGG + chr2_4:26918238-26918257 None:intergenic 30.0%
!! AAAACAAACGAGGTTTTATG+AGG + chr2_4:26918267-26918286 None:intergenic 30.0%
! TCATAAAACCTCGTTTGTTT+TGG - chr2_4:26918266-26918285 Msa0323240:intron 30.0%
!! AATAAGCATTGATGTCAAAC+GGG + chr2_4:26918307-26918326 None:intergenic 30.0%
!! CAATAAGCATTGATGTCAAA+CGG + chr2_4:26918308-26918327 None:intergenic 30.0%
GTTTGACATCAATGCTTATT+GGG - chr2_4:26918307-26918326 Msa0323240:intron 30.0%
ACATTGAGACTTATCCTATA+GGG + chr2_4:26918412-26918431 None:intergenic 30.0%
TACATTGAGACTTATCCTAT+AGG + chr2_4:26918413-26918432 None:intergenic 30.0%
! GTCGGTTAATTTACCAATTT+TGG - chr2_4:26918464-26918483 Msa0323240:CDS 30.0%
TAAATAGCAACTCCCAAAAT+TGG + chr2_4:26918480-26918499 None:intergenic 30.0%
!!! AATTTTTTTGTTTGGGGGAA+TGG + chr2_4:26918915-26918934 None:intergenic 30.0%
TTTGAATGAGATCACTTCAA+CGG - chr2_4:26919007-26919026 Msa0323240:CDS 30.0%
GGTGATACTTTATCATGTAT+AGG + chr2_4:26919071-26919090 None:intergenic 30.0%
TTCAAAATAATATGGCTGTG+AGG + chr2_4:26919092-26919111 None:intergenic 30.0%
CAGAGGAGTTCAAAATAATA+TGG + chr2_4:26919100-26919119 None:intergenic 30.0%
TTGTGAAAATTGCTTACATG+AGG + chr2_4:26919206-26919225 None:intergenic 30.0%
! TTGTAGAAAAGAGTGTGTGT+GGG + chr2_4:26916663-26916682 None:intergenic 35.0%
! TTTGTAGAAAAGAGTGTGTG+TGG + chr2_4:26916664-26916683 None:intergenic 35.0%
!!! CTTCTTTAGGGTTTTCAAGA+TGG + chr2_4:26916796-26916815 None:intergenic 35.0%
TCTTGAAAACCCTAAAGAAG+AGG - chr2_4:26916796-26916815 Msa0323240:CDS 35.0%
CAAAAGAAACAACTTCATCG+CGG + chr2_4:26916838-26916857 None:intergenic 35.0%
GTTCTGATGTTAACAATGCT+CGG - chr2_4:26916873-26916892 Msa0323240:CDS 35.0%
GAGTTTCTTCCTCTTAACAA+AGG + chr2_4:26917074-26917093 None:intergenic 35.0%
TCAACGGGAAAGTTATTTGT+TGG - chr2_4:26917127-26917146 Msa0323240:intron 35.0%
CAACGGGAAAGTTATTTGTT+GGG - chr2_4:26917128-26917147 Msa0323240:intron 35.0%
GAAATTGTTTACTATCCTCG+AGG - chr2_4:26917259-26917278 Msa0323240:intron 35.0%
AATTGTTTACTATCCTCGAG+GGG - chr2_4:26917261-26917280 Msa0323240:intron 35.0%
! TGATTGGTGTGATTTTGTAG+TGG - chr2_4:26917285-26917304 Msa0323240:intron 35.0%
TAGAGATTGTCCCTTGATAT+GGG + chr2_4:26917396-26917415 None:intergenic 35.0%
ATAGAGATTGTCCCTTGATA+TGG + chr2_4:26917397-26917416 None:intergenic 35.0%
TTCAGTAGAATCGTATGTGT+CGG - chr2_4:26917605-26917624 Msa0323240:intron 35.0%
! TGCAATATCGATTGGTGTAA+TGG + chr2_4:26918011-26918030 None:intergenic 35.0%
! TCAAACGGGTATTTTCATTG+TGG + chr2_4:26918293-26918312 None:intergenic 35.0%
CGTTTGACATCAATGCTTAT+TGG - chr2_4:26918306-26918325 Msa0323240:intron 35.0%
TCAAAGAGCAGTAACTAACT+AGG + chr2_4:26918356-26918375 None:intergenic 35.0%
GAGAACAAGTATGTTACTGT+CGG - chr2_4:26918446-26918465 Msa0323240:CDS 35.0%
TGAACAATATTGGTGCATTG+TGG - chr2_4:26918702-26918721 Msa0323240:CDS 35.0%
! TTGGGATGAATTTCAAGTCT+CGG - chr2_4:26918827-26918846 Msa0323240:CDS 35.0%
TGCCCAACTAAGTGATAAAA+AGG - chr2_4:26918953-26918972 Msa0323240:CDS 35.0%
!!! AAGAAGGAGAAGCGTTTTCT+AGG + chr2_4:26916625-26916644 None:intergenic 40.0%
! GTAATGGAGGAAGAAGAAGA+AGG + chr2_4:26916641-26916660 None:intergenic 40.0%
! AAAAGAGTGTGTGTGGGTAA+TGG + chr2_4:26916657-26916676 None:intergenic 40.0%
! AGATGGACATCGTTGGTTAT+TGG + chr2_4:26916779-26916798 None:intergenic 40.0%
!! GTTTTCAAGATGGACATCGT+TGG + chr2_4:26916786-26916805 None:intergenic 40.0%
GATTGGCAACCTCTTCTTTA+GGG + chr2_4:26916808-26916827 None:intergenic 40.0%
AGATTGGCAACCTCTTCTTT+AGG + chr2_4:26916809-26916828 None:intergenic 40.0%
TGAAACTTCACCATGAACGT+CGG + chr2_4:26916987-26917006 None:intergenic 40.0%
TGTTAAGAGGAAGAAACTCG+TGG - chr2_4:26917075-26917094 Msa0323240:intron 40.0%
! AGTTATTTGTTGGGATTCGC+AGG - chr2_4:26917137-26917156 Msa0323240:intron 40.0%
!! AGTAATGGAGGCATTGAAGT+TGG - chr2_4:26917216-26917235 Msa0323240:intron 40.0%
!! TGGTGTGATTTTGTAGTGGA+TGG - chr2_4:26917289-26917308 Msa0323240:intron 40.0%
TCTTCAAGGATCCCATATCA+AGG - chr2_4:26917382-26917401 Msa0323240:intron 40.0%
CTTCAAGGATCCCATATCAA+GGG - chr2_4:26917383-26917402 Msa0323240:intron 40.0%
TAATTTATGGCGCATGCTTC+AGG - chr2_4:26917432-26917451 Msa0323240:intron 40.0%
AACACACTCACAAACACACA+CGG - chr2_4:26917519-26917538 Msa0323240:intron 40.0%
ACATCCTCACTCTTGCAAAA+AGG + chr2_4:26917951-26917970 None:intergenic 40.0%
! TTTTTGCAAGAGTGAGGATG+TGG - chr2_4:26917950-26917969 Msa0323240:intron 40.0%
!! CAAACGAGGTTTTATGAGGA+AGG + chr2_4:26918263-26918282 None:intergenic 40.0%
ATTGTGGACCAAAACAAACG+AGG + chr2_4:26918277-26918296 None:intergenic 40.0%
CTAACTAGGATCGTCTCTAA+TGG + chr2_4:26918342-26918361 None:intergenic 40.0%
TATATTGCTTCGTGCCCTAT+AGG - chr2_4:26918395-26918414 Msa0323240:CDS 40.0%
TATTGGTGCATTGTGGCTAA+TGG - chr2_4:26918709-26918728 Msa0323240:CDS 40.0%
! TGCATTGTGGCTAATGGTTT+TGG - chr2_4:26918715-26918734 Msa0323240:CDS 40.0%
! GCATTGTGGCTAATGGTTTT+GGG - chr2_4:26918716-26918735 Msa0323240:CDS 40.0%
AATGAGATCACTTCAACGGA+AGG - chr2_4:26919011-26919030 Msa0323240:CDS 40.0%
ATGAGATCACTTCAACGGAA+GGG - chr2_4:26919012-26919031 Msa0323240:CDS 40.0%
TCTCATATCAAGGGAGATGA+TGG - chr2_4:26919227-26919246 Msa0323240:CDS 40.0%
GCACATGTGTGCCAAATTTC+TGG + chr2_4:26916542-26916561 None:intergenic 45.0%
!!! CTTTGGAATTTTGACGGCAG+CGG + chr2_4:26916567-26916586 None:intergenic 45.0%
! CGTTTTCTAGGTGACCTTGT+GGG + chr2_4:26916613-26916632 None:intergenic 45.0%
GAGACGATACAAGGAGTGAA+AGG + chr2_4:26916695-26916714 None:intergenic 45.0%
TCAACAGCTGAGACGATACA+AGG + chr2_4:26916704-26916723 None:intergenic 45.0%
TTCATCGCGGTTGTTGAGAT+TGG + chr2_4:26916825-26916844 None:intergenic 45.0%
ACATTATCGGCACAAAAGCG+AGG + chr2_4:26916911-26916930 None:intergenic 45.0%
! AAGTTTTACCATGTCTGCCG+CGG - chr2_4:26917007-26917026 Msa0323240:intron 45.0%
! GAAGAAGGATGAGTTGGAGA+AGG - chr2_4:26917192-26917211 Msa0323240:intron 45.0%
! TGAGTTGGAGAAGGCAGTAA+TGG - chr2_4:26917201-26917220 Msa0323240:intron 45.0%
!! AATGGAGGCATTGAAGTTGG+CGG - chr2_4:26917219-26917238 Msa0323240:intron 45.0%
TCCTAATGTCGGACACACAT+CGG + chr2_4:26917640-26917659 None:intergenic 45.0%
CCTCACTCTTGCAAAAAGGT+AGG + chr2_4:26917947-26917966 None:intergenic 45.0%
! CCTACCTTTTTGCAAGAGTG+AGG - chr2_4:26917944-26917963 Msa0323240:intron 45.0%
AAATAACCTCAACCCACCAC+GGG + chr2_4:26918872-26918891 None:intergenic 45.0%
GAAATAACCTCAACCCACCA+CGG + chr2_4:26918873-26918892 None:intergenic 45.0%
CGAAGGAAACTTTGTCTCAG+AGG + chr2_4:26919117-26919136 None:intergenic 45.0%
GTGTTGCAATTGTGAGTCGA+AGG + chr2_4:26919134-26919153 None:intergenic 45.0%
! GATGATGGCTATAAGAGCTG+CGG - chr2_4:26919242-26919261 Msa0323240:CDS 45.0%
!!! ATATTTTAATATATCAATAA+TGG - chr2_4:26918207-26918226 Msa0323240:intron 5.0%
!!! TATTTTAATATATCAATAAT+GGG - chr2_4:26918208-26918227 Msa0323240:intron 5.0%
! ATTTCTGGACGCACATGCGA+TGG + chr2_4:26916527-26916546 None:intergenic 50.0%
CGCATGTGCGTCCAGAAATT+TGG - chr2_4:26916528-26916547 Msa0323240:CDS 50.0%
! GCGTTTTCTAGGTGACCTTG+TGG + chr2_4:26916614-26916633 None:intergenic 50.0%
! AGAGTGTGTGTGGGTAATGG+AGG + chr2_4:26916654-26916673 None:intergenic 50.0%
ACAAAGACCTCATCCGTGTG+AGG + chr2_4:26916740-26916759 None:intergenic 50.0%
AAGCCGCGGGAAGACATTAT+CGG + chr2_4:26916924-26916943 None:intergenic 50.0%
!! CAGGTGTTGAGACGAGGATT+CGG + chr2_4:26916957-26916976 None:intergenic 50.0%
!! AACGAACAGGTGTTGAGACG+AGG + chr2_4:26916963-26916982 None:intergenic 50.0%
CATGAACGTCGGTAACGAAC+AGG + chr2_4:26916976-26916995 None:intergenic 50.0%
CTGTTCGTTACCGACGTTCA+TGG - chr2_4:26916974-26916993 Msa0323240:intron 50.0%
! AGTTTTACCATGTCTGCCGC+GGG - chr2_4:26917008-26917027 Msa0323240:intron 50.0%
TGTTTCTCTTGGGAAACCCG+CGG + chr2_4:26917027-26917046 None:intergenic 50.0%
AAGAGGAAGAAACTCGTGGC+TGG - chr2_4:26917079-26917098 Msa0323240:intron 50.0%
!! GAACACGCAGTTTGTTGCAG+CGG - chr2_4:26917159-26917178 Msa0323240:intron 50.0%
GGAGCAGAAGAAGGATGAGT+TGG - chr2_4:26917186-26917205 Msa0323240:intron 50.0%
GTTGGAGAAGGCAGTAATGG+AGG - chr2_4:26917204-26917223 Msa0323240:intron 50.0%
GAAGTTGGCGGAAGAGAACA+AGG - chr2_4:26917231-26917250 Msa0323240:intron 50.0%
ACTATCCTCGAGGGGATGAT+TGG - chr2_4:26917269-26917288 Msa0323240:intron 50.0%
ACCGATGTGTGTCCGACATT+AGG - chr2_4:26917636-26917655 Msa0323240:intron 50.0%
TTTGTTTGGGGGAATGGCGT+CGG + chr2_4:26918909-26918928 None:intergenic 50.0%
TCTCCTTGCCGATCCTCACA+CGG - chr2_4:26916724-26916743 Msa0323240:CDS 55.0%
GACCTCATCCGTGTGAGGAT+CGG + chr2_4:26916735-26916754 None:intergenic 55.0%
CGGCACAAAAGCGAGGTATG+TGG + chr2_4:26916904-26916923 None:intergenic 55.0%
! GTGCCGATAATGTCTTCCCG+CGG - chr2_4:26916918-26916937 Msa0323240:CDS 55.0%
TCACACCAATCATCCCCTCG+AGG + chr2_4:26917277-26917296 None:intergenic 55.0%
TTCCAAGACGAGTCAACCCG+TGG - chr2_4:26918853-26918872 Msa0323240:CDS 55.0%
AAGACGAGTCAACCCGTGGT+GGG - chr2_4:26918857-26918876 Msa0323240:CDS 55.0%
CATCCGTGTGAGGATCGGCA+AGG + chr2_4:26916730-26916749 None:intergenic 60.0%
TGCCGATCCTCACACGGATG+AGG - chr2_4:26916730-26916749 Msa0323240:CDS 60.0%
GAGACGAGGATTCGGCCTCT+AGG + chr2_4:26916949-26916968 None:intergenic 60.0%
!! CACGCAGTTTGTTGCAGCGG+CGG - chr2_4:26917162-26917181 Msa0323240:intron 60.0%
!! CACCACGGGTTGACTCGTCT+TGG + chr2_4:26918858-26918877 None:intergenic 60.0%
CAAGACGAGTCAACCCGTGG+TGG - chr2_4:26918856-26918875 Msa0323240:CDS 60.0%
!! AGTCAACCCGTGGTGGGTTG+AGG - chr2_4:26918863-26918882 Msa0323240:CDS 60.0%
CTTCCCGCGGCTTGACCTAG+AGG - chr2_4:26916931-26916950 Msa0323240:CDS 65.0%
TCGGCCTCTAGGTCAAGCCG+CGG + chr2_4:26916938-26916957 None:intergenic 65.0%
TGGGAAACCCGCGGCAGACA+TGG + chr2_4:26917018-26917037 None:intergenic 65.0%
!! GCAGTTTGTTGCAGCGGCGG+CGG - chr2_4:26917165-26917184 Msa0323240:intron 65.0%
CGGCCTCTAGGTCAAGCCGC+GGG + chr2_4:26916937-26916956 None:intergenic 70.0%
AGCGGCGGCGGAGCAGAAGA+AGG - chr2_4:26917177-26917196 Msa0323240:intron 70.0%
! TGGGGGAATGGCGTCGGAGC+AGG + chr2_4:26918903-26918922 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 26916506 26919277 26916506 ID=Msa0323240;Name=Msa0323240
chr2_4 mRNA 26916506 26919277 26916506 ID=Msa0323240-mRNA-1;Parent=Msa0323240;Name=Msa0323240-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|0|0|1|1|1|2|0|467
chr2_4 exon 26918330 26919277 26918330 ID=Msa0323240-mRNA-1:exon:9407;Parent=Msa0323240-mRNA-1
chr2_4 exon 26916506 26916961 26916506 ID=Msa0323240-mRNA-1:exon:9406;Parent=Msa0323240-mRNA-1
chr2_4 CDS 26918330 26919277 26918330 ID=Msa0323240-mRNA-1:cds;Parent=Msa0323240-mRNA-1
chr2_4 CDS 26916506 26916961 26916506 ID=Msa0323240-mRNA-1:cds;Parent=Msa0323240-mRNA-1
Gene Sequence

>Msa0323240

ATGTCTGTTCAGAAGCAACCATCGCATGTGCGTCCAGAAATTTGGCACACATGTGCTGCCGCTGCCGTCAAAATTCCAAAGTTGCATTCAAGAGTTTATTATTTCCCACAAGGTCACCTAGAAAACGCTTCTCCTTCTTCTTCTTCCTCCATTACCCACACACACTCTTTTCTACAAAGTTTTCGTCCTTTCACTCCTTGTATCGTCTCAGCTGTTGATCTCCTTGCCGATCCTCACACGGATGAGGTCTTTGTTAAACTGCTACTTACTCCAATAACCAACGATGTCCATCTTGAAAACCCTAAAGAAGAGGTTGCCAATCTCAACAACCGCGATGAAGTTGTTTCTTTTGTCAAGATTCTCACTCGTTCTGATGTTAACAATGCTCGGTCATTCCACATACCTCGCTTTTGTGCCGATAATGTCTTCCCGCGGCTTGACCTAGAGGCCGAATCCTCGTCTCAACACCTGTTCGTTACCGACGTTCATGGTGAAGTTTCAAAGTTTTACCATGTCTGCCGCGGGTTTCCCAAGAGAAACATGTTATATATCTCTGAATGGAATTCCTTTGTTAAGAGGAAGAAACTCGTGGCTGGAGATTCCGTTATTTTCATGAAAGACTCAACGGGAAAGTTATTTGTTGGGATTCGCAGGAACACGCAGTTTGTTGCAGCGGCGGCGGAGCAGAAGAAGGATGAGTTGGAGAAGGCAGTAATGGAGGCATTGAAGTTGGCGGAAGAGAACAAGGCATTTGAAATTGTTTACTATCCTCGAGGGGATGATTGGTGTGATTTTGTAGTGGATGGTAATGCTGTTGATGAATCAATGAAGATTCAATGGAACCCTAGAATGAGAGTTAAGATGAAGACTGATAAATCTTCAAGGATCCCATATCAAGGGACAATCTCTATTGTATCTCGTACTTCTAATTTATGGCGCATGCTTCAGGTTAATTGGGATGAATTTCAAGTCTCGGAAATTCCAAGACGAGTCAACCCGTGGTGGGTTGAGGTTATTTCTCACAAACCTGCTCCGACGCCATTCCCCCAAACAAAAAAATTTAGAACTACTCAAAGTTCTGCCCAACTAAGTGATAAAAAGGAAACATTATTGAATGATGATGCTTCAAACATTTTGAATGAGATCACTTCAACGGAAGGGTTAAGTAATGTGTCAAAAGAATTAAACATGAGTCCTATACATGATAAAGTATCACCTCACAGCCATATTATTTTGAACTCCTCTGAGACAAAGTTTCCTTCGACTCACAATTGCAACACCAAAAATTATAGTGATGGTTCAATTATGTTATTTGGTAAGATCATACAGCCTCATGTAAGCAATTTTCACAATTCTCATATCAAGGGAGATGATGGCTATAAGAGCTGCGGTGAAATCCATTAA
Protein sequence

>Msa0323240

MSVQKQPSHVRPEIWHTCAAAAVKIPKLHSRVYYFPQGHLENASPSSSSSITHTHSFLQSFRPFTPCIVSAVDLLADPHTDEVFVKLLLTPITNDVHLENPKEEVANLNNRDEVVSFVKILTRSDVNNARSFHIPRFCADNVFPRLDLEAESSSQHLFVTDVHGEVSKFYHVCRGFPKRNMLYISEWNSFVKRKKLVAGDSVIFMKDSTGKLFVGIRRNTQFVAAAAEQKKDELEKAVMEALKLAEENKAFEIVYYPRGDDWCDFVVDGNAVDESMKIQWNPRMRVKMKTDKSSRIPYQGTISIVSRTSNLWRMLQVNWDEFQVSEIPRRVNPWWVEVISHKPAPTPFPQTKKFRTTQSSAQLSDKKETLLNDDASNILNEITSTEGLSNVSKELNMSPIHDKVSPHSHIILNSSETKFPSTHNCNTKNYSDGSIMLFGKIIQPHVSNFHNSHIKGDDGYKSCGEIH*