AlfalfaGEDB | Msa0323350

M. sativa cultivar zhongmu-4 / Msa0323350

KEGG: -
GO: GO:0000976 GO:0000987 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0009555 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009734 GO:0009755 GO:0009889 GO:0009987 GO:0010033 GO:0010208 GO:0010468 GO:0010556 GO:0010927 GO:0016043 GO:0019219 GO:0019222 GO:0022607 GO:0022622 GO:0023052 GO:0030198 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0032870 GO:0032989 GO:0033036 GO:0033037 GO:0042221 GO:0042545 GO:0043062 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044085 GO:0044212 GO:0044424 GO:0044464 GO:0045229 GO:0048229 GO:0048364 GO:0048646 GO:0048731 GO:0048830 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051179 GO:0051252 GO:0051641 GO:0051716 GO:0052386 GO:0052543 GO:0052545 GO:0060255 GO:0065007 GO:0070727 GO:0070887 GO:0071310 GO:0071365 GO:0071495 GO:0071554 GO:0071555 GO:0071840 GO:0080090 GO:0085029 GO:0097159 GO:0099402 GO:0140110 GO:1901363 GO:1903506 GO:1990837 GO:2000112 GO:2001141
PFAM: PF02362.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0323350	XP_003592973.1	86.486	481	35	5	1	476	1	456	0.0	832

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0323350	sp\|Q84WU6\|ARFQ_ARATH	37.653	409	198	11	9	370	15	413	3.94e-84	273

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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TFs/TRs:

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Gene ID	Type	Classification
Msa0323350	TF	B3-ARF

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0323350	MtrunA17_Chr2g0277461	86.486	481	35	5	1	476	1	456	0.0	832
Msa0323350	MtrunA17_Chr2g0277531	72.152	316	72	6	7	317	14	318	1.63e-160	457
Msa0323350	MtrunA17_Chr1g0178011	49.891	459	198	10	6	455	5	440	3.30e-134	395
Msa0323350	MtrunA17_Chr5g0424931	45.665	519	204	13	7	458	13	520	1.34e-133	396
Msa0323350	MtrunA17_Chr5g0417281	44.015	518	218	13	3	458	1	508	3.07e-120	362
Msa0323350	MtrunA17_Chr5g0436761	43.376	468	239	13	5	459	4	458	1.61e-107	327
Msa0323350	MtrunA17_Chr5g0417211	42.765	463	191	12	5	459	4	400	4.31e-103	314
Msa0323350	MtrunA17_Chr2g0289221	52.516	318	136	6	6	317	13	321	9.95e-103	311
Msa0323350	MtrunA17_Chr3g0115271	35.149	569	257	17	5	476	11	564	5.00e-93	293
Msa0323350	MtrunA17_Chr5g0425261	45.198	354	164	13	7	346	6	343	5.62e-89	276

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0323350	AT1G77850.2	37.653	409	198	11	9	370	15	413	3.97e-85	273
Msa0323350	AT1G77850.1	37.653	409	198	11	9	370	15	413	4.01e-85	273
Msa0323350	AT4G30080.1	37.157	401	187	13	12	358	18	407	1.24e-73	245
Msa0323350	AT2G28350.2	35.749	414	195	12	5	358	2	404	1.47e-72	242
Msa0323350	AT2G28350.1	35.749	414	195	12	5	358	2	404	2.18e-72	242
Msa0323350	AT4G23980.2	33.731	335	208	6	13	337	12	342	1.16e-49	180
Msa0323350	AT4G23980.1	33.731	335	208	6	13	337	12	342	1.32e-49	179
Msa0323350	AT5G37020.2	31.806	371	238	7	13	370	22	390	2.88e-48	177
Msa0323350	AT5G37020.1	31.806	371	238	7	13	370	22	390	4.27e-48	177
Msa0323350	AT1G30330.3	33.428	353	214	9	13	349	25	372	3.87e-47	175

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Find 100 sgRNAs with CRISPR-Local

Find 182 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAGTGTGTGTGGGTAA+TGG	0.489755	2_4:-27008500	None:intergenic
AAATAACCTCAACCCACCAC+GGG	0.608942	2_4:-27010675	None:intergenic
AAATTGTTTACTATCCTCGA+GGG	0.568194	2_4:+27009106	Msa0323350:CDS
AACGAACAGGTGTTGAGACG+AGG	0.644655	2_4:-27008806	None:intergenic
AAGACGAGTCAACCCGTGGT+GGG	0.646955	2_4:+27010663	Msa0323350:CDS
AAGAGGAAGAAACTCGTGGC+TGG	0.538632	2_4:+27008925	Msa0323350:CDS
AAGCCGCGGGAAGACATTAT+CGG	0.516617	2_4:-27008767	None:intergenic
AATGAGATCACTTCAACGGA+AGG	0.592309	2_4:+27010817	Msa0323350:CDS
AATGGAATTCGTTTGTTAAG+AGG	0.398429	2_4:+27008908	Msa0323350:CDS
AATGGAGGCATTGAAGTTGG+CGG	0.590740	2_4:+27009065	Msa0323350:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TAAACTCTTGAATGCAACTT+TGG	-	chr2_4:27008430-27008449	None:intergenic	30.0%
AGAGTTTATTATTTCCCACA+AGG	+	chr2_4:27008442-27008461	Msa0323350:CDS	30.0%
AAATTGTTTACTATCCTCGA+GGG	+	chr2_4:27009106-27009125	Msa0323350:CDS	30.0%
TCATCTTAACTCTCATTCTA+GGG	-	chr2_4:27009197-27009216	None:intergenic	30.0%
TTCATCTTAACTCTCATTCT+AGG	-	chr2_4:27009198-27009217	None:intergenic	30.0%
TGAAGACTGATAAATCTTCA+AGG	+	chr2_4:27009214-27009233	Msa0323350:CDS	30.0%
GTGTTTCATAGCTAGATAAT+TGG	-	chr2_4:27009350-27009369	None:intergenic	30.0%
TATTAAACGTCTCCTAATGT+CGG	-	chr2_4:27009497-27009516	None:intergenic	30.0%
ATACTAGATACTATGAAGCA+CGG	-	chr2_4:27009528-27009547	None:intergenic	30.0%
CTGTAATAATCTCTCTATGT+GGG	-	chr2_4:27010040-27010059	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_4	gene	27008352	27011110	27008352	ID=Msa0323350;Name=Msa0323350
chr2_4	mRNA	27008352	27011110	27008352	ID=Msa0323350-mRNA-1;Parent=Msa0323350;Name=Msa0323350-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0\|0\|0\|1\|1\|1\|2\|0\|476
chr2_4	exon	27008352	27009299	27008352	ID=Msa0323350-mRNA-1:exon:9446;Parent=Msa0323350-mRNA-1
chr2_4	exon	27010628	27011110	27010628	ID=Msa0323350-mRNA-1:exon:9447;Parent=Msa0323350-mRNA-1
chr2_4	CDS	27008352	27009299	27008352	ID=Msa0323350-mRNA-1:cds;Parent=Msa0323350-mRNA-1
chr2_4	CDS	27010628	27011110	27010628	ID=Msa0323350-mRNA-1:cds;Parent=Msa0323350-mRNA-1

Gene Sequence

>Msa0323350

ATGTCTGTTCAGAAGCAACCATCGCATGTGCGTCCAGAAATTTGGCACACATGTGCTGCCGCTGCCGTCAAAATTCCAAAGTTGCATTCAAGAGTTTATTATTTCCCACAAGGTCACCTAGAAAACGCTTCTCCTTCTTCTTCTTCCTCCATTACCCACACACACTCTTTTCTACAAAGTTTTCGTCCTTTCACTCCTTGTATCGTCTCAGCTGTTGATCTCCTTGCCGATCCTCACACGGATGAGGTCTTTGTTAAACTGCTACTTACTCCAATAACCAACGATGTCCATCTTGAAAACCCTAAAGAAGAGGTTGCCAATCTCAACAACCGCGATGAAGTTGTTTCTTTTGTCAAGATTCTCACTCGTTCTGATGTTAACAATGCTCGGTCATTCCACATACCTCGCTTTTGTGCCGATAATGTCTTCCCGCGGCTTGACCTAGAGGCCGAATCCTCGTCTCAACACCTGTTCGTTACCGACGTTCATGGTGAAGTTTCAAAGTTTTACCATGTCTGCCGCGGGTTTCCCAAGAGAAACATGTTATATATCTCTGAATGGAATTCGTTTGTTAAGAGGAAGAAACTCGTGGCTGGAGATTCCGTTATTTTCATGAAAGACTCAACGGGAAAGTTATTTGTTGGGATTCGCAGGAACACGCAGTTTGTTGCAGCGGCGGCGGAGCAGAAGAAGGATGAGTTGGAGAAGGCAGTAATGGAGGCATTGAAGTTGGCGGAAGAGAACAAGGCATTTGAAATTGTTTACTATCCTCGAGGGGATGATTGGTGTGATTTTGTAGTGGATGGTAATGCTGTTGATGAATCAATGAAGATTCAATGGAACCCTAGAATGAGAGTTAAGATGAAGACTGATAAATCTTCAAGGATCCCATATCAAGGGACAATCTCTATTGTATCTCGTACTTCTAATTTATGGCGCATGCTTCAGGTTAATTGGGATGAATTTCAAGTCTCGGAAATTCCAAGACGAGTCAACCCGTGGTGGGTTGAGGTTATTTCTCACAAACCTGCTCCGACGCCATTCCCCCAAACAAAAAAATTTAGAACTACTCAAAGTTCTGCCCAACTAAGTGATAAAAAGGAAACATTATTGAATGATGATGCTTCAAACATTTTGAATGAGATCACTTCAACGGAAGGGTTAAGTAATGTGTCAAAAGAATTAAACATGAGTCCTATACATGATAAAGTATCACCTCACAGCCATATTATTTTGAACTCCTCTGAGACAAAGTTTCCTTCGACTCACAATTGCAACACCAAAAATTATAGTGATGGTTCAATTATGTTATTTGGTAAGATCATACAGCCTCATGTAAGCAATTTTCACAATTCTCATATCAAGGGAGATGATGGCTATATAAGAGCTGCGGTGAAATCCATTAACAAAAAGTTTGGTGTAAAGAACTAG

Protein sequence

>Msa0323350

MSVQKQPSHVRPEIWHTCAAAAVKIPKLHSRVYYFPQGHLENASPSSSSSITHTHSFLQSFRPFTPCIVSAVDLLADPHTDEVFVKLLLTPITNDVHLENPKEEVANLNNRDEVVSFVKILTRSDVNNARSFHIPRFCADNVFPRLDLEAESSSQHLFVTDVHGEVSKFYHVCRGFPKRNMLYISEWNSFVKRKKLVAGDSVIFMKDSTGKLFVGIRRNTQFVAAAAEQKKDELEKAVMEALKLAEENKAFEIVYYPRGDDWCDFVVDGNAVDESMKIQWNPRMRVKMKTDKSSRIPYQGTISIVSRTSNLWRMLQVNWDEFQVSEIPRRVNPWWVEVISHKPAPTPFPQTKKFRTTQSSAQLSDKKETLLNDDASNILNEITSTEGLSNVSKELNMSPIHDKVSPHSHIILNSSETKFPSTHNCNTKNYSDGSIMLFGKIIQPHVSNFHNSHIKGDDGYIRAAVKSINKKFGVKN*