AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0323350

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0323350 MtrunA17_Chr2g0277461 86.486 481 35 5 1 476 1 456 0.0 832
Msa0323350 MtrunA17_Chr2g0277531 72.152 316 72 6 7 317 14 318 1.63e-160 457
Msa0323350 MtrunA17_Chr1g0178011 49.891 459 198 10 6 455 5 440 3.30e-134 395
Msa0323350 MtrunA17_Chr5g0424931 45.665 519 204 13 7 458 13 520 1.34e-133 396
Msa0323350 MtrunA17_Chr5g0417281 44.015 518 218 13 3 458 1 508 3.07e-120 362
Msa0323350 MtrunA17_Chr5g0436761 43.376 468 239 13 5 459 4 458 1.61e-107 327
Msa0323350 MtrunA17_Chr5g0417211 42.765 463 191 12 5 459 4 400 4.31e-103 314
Msa0323350 MtrunA17_Chr2g0289221 52.516 318 136 6 6 317 13 321 9.95e-103 311
Msa0323350 MtrunA17_Chr3g0115271 35.149 569 257 17 5 476 11 564 5.00e-93 293
Msa0323350 MtrunA17_Chr5g0425261 45.198 354 164 13 7 346 6 343 5.62e-89 276
Msa0323350 MtrunA17_Chr5g0424801 45.042 353 164 13 7 344 6 343 5.80e-89 276
Msa0323350 MtrunA17_Chr5g0424741 41.388 418 176 15 6 363 5 413 1.08e-80 259
Msa0323350 MtrunA17_Chr2g0326761 39.152 401 179 12 12 358 10 399 5.70e-80 262
Msa0323350 MtrunA17_Chr4g0028971 36.740 411 191 11 4 355 3 403 5.03e-76 251
Msa0323350 MtrunA17_Chr1g0181211 35.075 402 191 11 12 353 26 417 7.31e-74 246
Msa0323350 MtrunA17_Chr1g0199681 38.035 397 186 13 13 358 94 481 2.60e-73 244
Msa0323350 MtrunA17_Chr7g0264171 35.507 414 191 15 10 358 28 430 2.93e-68 229
Msa0323350 MtrunA17_Chr7g0264161 39.617 313 158 12 23 317 2 301 2.11e-59 197
Msa0323350 MtrunA17_Chr5g0425661 49.268 205 93 4 13 214 37 233 3.30e-56 187
Msa0323350 MtrunA17_Chr8g0385791 32.398 392 247 9 13 391 59 445 1.31e-50 184
Msa0323350 MtrunA17_Chr8g0376771 33.711 353 212 9 13 349 23 369 3.47e-48 177
Msa0323350 MtrunA17_Chr4g0070181 33.333 354 209 10 10 348 20 361 3.50e-48 178
Msa0323350 MtrunA17_Chr2g0282961 30.747 348 205 9 13 337 53 387 1.55e-47 173
Msa0323350 MtrunA17_Chr2g0285461 32.670 352 218 8 13 349 23 370 2.39e-47 175
Msa0323350 MtrunA17_Chr2g0301251 32.768 354 211 10 10 348 20 361 3.34e-47 175
Msa0323350 MtrunA17_Chr3g0109391 31.842 380 239 8 13 379 26 398 4.20e-47 174
Msa0323350 MtrunA17_Chr5g0433301 32.295 353 218 8 13 349 24 371 6.09e-46 171
Msa0323350 MtrunA17_Chr2g0276781 32.500 360 218 9 13 356 23 373 3.14e-45 167
Msa0323350 MtrunA17_Chr1g0156321 33.529 340 207 9 13 338 43 377 3.23e-45 169
Msa0323350 MtrunA17_Chr4g0046371 32.044 362 196 11 8 337 33 376 5.98e-44 164
Msa0323350 MtrunA17_Chr8g0389081 32.479 351 217 10 5 340 24 369 4.67e-43 163
Msa0323350 MtrunA17_Chr4g0009751 30.055 366 237 9 13 365 19 378 1.60e-41 156
Msa0323350 MtrunA17_Chr8g0346711 31.065 338 212 9 13 337 29 358 9.90e-40 152
Msa0323350 MtrunA17_Chr7g0239061 28.647 377 243 9 13 373 14 380 1.27e-39 152
Msa0323350 MtrunA17_Chr4g0029671 30.435 368 228 10 13 356 54 417 1.12e-37 146
Msa0323350 MtrunA17_Chr2g0326281 30.278 360 212 8 13 337 56 411 1.37e-37 146
Msa0323350 MtrunA17_Chr5g0419861 58.333 120 40 6 51 165 9 123 6.29e-33 121
Msa0323350 MtrunA17_Chr5g0425651 48.780 123 51 6 239 350 1 122 1.66e-24 98.2
Msa0323350 MtrunA17_Chr5g0424791 41.830 153 71 5 66 214 13 151 1.39e-23 96.7
Msa0323350 MtrunA17_Chr8g0356211 27.928 222 136 8 131 337 8 220 3.87e-17 82.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0323350 AT1G77850.2 37.653 409 198 11 9 370 15 413 3.97e-85 273
Msa0323350 AT1G77850.1 37.653 409 198 11 9 370 15 413 4.01e-85 273
Msa0323350 AT4G30080.1 37.157 401 187 13 12 358 18 407 1.24e-73 245
Msa0323350 AT2G28350.2 35.749 414 195 12 5 358 2 404 1.47e-72 242
Msa0323350 AT2G28350.1 35.749 414 195 12 5 358 2 404 2.18e-72 242
Msa0323350 AT4G23980.2 33.731 335 208 6 13 337 12 342 1.16e-49 180
Msa0323350 AT4G23980.1 33.731 335 208 6 13 337 12 342 1.32e-49 179
Msa0323350 AT5G37020.2 31.806 371 238 7 13 370 22 390 2.88e-48 177
Msa0323350 AT5G37020.1 31.806 371 238 7 13 370 22 390 4.27e-48 177
Msa0323350 AT1G30330.3 33.428 353 214 9 13 349 25 372 3.87e-47 175
Msa0323350 AT1G30330.2 33.428 353 214 9 13 349 25 372 3.87e-47 175
Msa0323350 AT1G30330.1 33.428 353 214 9 13 349 23 370 4.19e-47 175
Msa0323350 AT5G62000.4 32.266 406 253 11 13 403 61 459 5.20e-47 174
Msa0323350 AT5G62000.2 34.349 361 213 12 13 356 61 414 8.06e-47 174
Msa0323350 AT5G62000.5 34.349 361 213 12 13 356 61 414 8.06e-47 174
Msa0323350 AT5G62000.3 34.349 361 213 12 13 356 61 414 8.06e-47 174
Msa0323350 AT5G62000.1 34.349 361 213 12 13 356 61 414 8.06e-47 174
Msa0323350 AT5G20730.3 32.687 361 217 9 3 348 14 363 1.22e-46 174
Msa0323350 AT5G20730.1 32.687 361 217 9 3 348 14 363 1.34e-46 174
Msa0323350 AT2G33860.1 31.988 347 202 9 13 337 54 388 1.58e-46 171
Msa0323350 AT2G46530.1 31.421 366 229 9 13 364 21 378 2.53e-46 170
Msa0323350 AT2G46530.3 31.421 366 229 9 13 364 42 399 4.09e-46 169
Msa0323350 AT5G20730.2 32.687 361 216 10 3 348 14 362 4.82e-46 172
Msa0323350 AT1G34310.1 33.237 346 200 9 8 337 22 352 5.01e-45 166
Msa0323350 AT3G61830.1 31.680 363 218 10 13 356 25 376 5.45e-45 166
Msa0323350 AT1G19850.2 34.294 347 202 11 10 338 40 378 2.46e-44 167
Msa0323350 AT1G19850.1 34.294 347 202 11 10 338 51 389 2.97e-44 167
Msa0323350 AT1G34390.1 32.659 346 200 10 8 337 22 350 8.86e-44 163
Msa0323350 AT1G35520.1 32.174 345 215 8 3 337 17 352 9.13e-44 162
Msa0323350 AT5G60450.2 31.445 353 212 8 8 337 36 381 1.85e-43 164
Msa0323350 AT1G19220.1 30.833 360 220 10 4 348 16 361 2.01e-43 164
Msa0323350 AT5G60450.1 31.609 348 208 8 13 337 65 405 4.20e-43 163
Msa0323350 AT1G35540.1 32.456 342 208 10 8 337 22 352 2.25e-42 159
Msa0323350 AT1G35240.1 32.353 340 211 8 8 337 22 352 3.77e-42 158
Msa0323350 AT1G34410.1 31.765 340 213 8 8 337 22 352 1.45e-41 157
Msa0323350 AT1G59750.4 31.339 351 222 9 13 350 22 366 1.86e-40 154
Msa0323350 AT1G59750.1 31.339 351 222 9 13 350 22 366 2.11e-40 154
Msa0323350 AT1G59750.3 31.339 351 222 9 13 350 22 366 2.11e-40 154
Msa0323350 AT1G59750.2 31.339 351 222 9 13 350 22 366 2.23e-40 154
Msa0323350 AT1G34170.1 30.372 349 222 9 1 337 13 352 5.08e-40 151
Msa0323350 AT1G34170.2 29.942 344 204 8 1 337 13 326 1.26e-37 144
Msa0323350 AT1G34170.3 29.942 344 204 8 1 337 13 326 4.29e-37 143
Msa0323350 AT3G61830.2 31.392 309 182 10 67 356 28 325 5.20e-32 129
Msa0323350 AT5G37020.3 32.584 267 165 7 117 370 31 295 3.80e-31 127
Msa0323350 AT1G43950.1 34.259 216 121 5 8 217 22 222 5.41e-30 117
Msa0323350 AT2G33860.2 32.479 234 141 6 117 337 2 231 6.70e-30 122
Msa0323350 AT2G46530.2 31.679 262 164 7 114 364 34 291 3.70e-29 120
Msa0323350 ATMG00940.1 43.210 81 42 3 236 313 65 144 9.13e-11 60.8

Find 100 sgRNAs with CRISPR-Local

Find 182 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATCTCGTACTTCTAATTTA+TGG 0.108193 2_4:+27009265 Msa0323350:CDS
TTTGAATATCGTAGGTTAAT+TGG 0.128409 2_4:+27010614 Msa0323350:intron
GATGGTTCAATTATGTTATT+TGG 0.174464 2_4:+27010973 Msa0323350:CDS
GCACATGTGTGCCAAATTTC+TGG 0.215696 2_4:-27008385 None:intergenic
CGCATGTGCGTCCAGAAATT+TGG 0.217939 2_4:+27008374 Msa0323350:CDS
TTCATCTTAACTCTCATTCT+AGG 0.234993 2_4:-27009195 None:intergenic
GATTGGCAACCTCTTCTTTA+GGG 0.275578 2_4:-27008651 None:intergenic
AGATTGGCAACCTCTTCTTT+AGG 0.275924 2_4:-27008652 None:intergenic
TAATTTATGGCGCATGCTTC+AGG 0.288610 2_4:+27009278 Msa0323350:CDS
ATAGAGATTGTCCCTTGATA+TGG 0.294890 2_4:-27009240 None:intergenic
AGATGGACATCGTTGGTTAT+TGG 0.309930 2_4:-27008622 None:intergenic
TTGAATATCGTAGGTTAATT+GGG 0.314702 2_4:+27010615 Msa0323350:intron
TGCCCAACTAAGTGATAAAA+AGG 0.319098 2_4:+27010759 Msa0323350:CDS
CTGTTCGTTACCGACGTTCA+TGG 0.320691 2_4:+27008820 Msa0323350:CDS
TTCATCGCGGTTGTTGAGAT+TGG 0.361556 2_4:-27008668 None:intergenic
TAAACTCTTGAATGCAACTT+TGG 0.370692 2_4:-27008427 None:intergenic
GATATATAACATGTTTCTCT+TGG 0.384809 2_4:-27008881 None:intergenic
AATGGAATTCGTTTGTTAAG+AGG 0.398429 2_4:+27008908 Msa0323350:CDS
CAGAGGAGTTCAAAATAATA+TGG 0.402834 2_4:-27010903 None:intergenic
TCAACGGGAAAGTTATTTGT+TGG 0.427846 2_4:+27008973 Msa0323350:CDS
CAACGGGAAAGTTATTTGTT+GGG 0.431447 2_4:+27008974 Msa0323350:CDS
GCAGTTTGTTGCAGCGGCGG+CGG 0.440577 2_4:+27009011 Msa0323350:CDS
TGAGTTGGAGAAGGCAGTAA+TGG 0.441158 2_4:+27009047 Msa0323350:CDS
TCATCTTAACTCTCATTCTA+GGG 0.445548 2_4:-27009194 None:intergenic
ATGAATCAATGAAGATTCAA+TGG 0.450458 2_4:+27009169 Msa0323350:CDS
TGAGTCTTTCATGAAAATAA+CGG 0.452973 2_4:-27008953 None:intergenic
TGAAGACTGATAAATCTTCA+AGG 0.454632 2_4:+27009214 Msa0323350:CDS
CAGGTGTTGAGACGAGGATT+CGG 0.457588 2_4:-27008800 None:intergenic
TTGGGATGAATTTCAAGTCT+CGG 0.458166 2_4:+27010633 Msa0323350:CDS
ACTATCCTCGAGGGGATGAT+TGG 0.466368 2_4:+27009115 Msa0323350:CDS
AGTCAACCCGTGGTGGGTTG+AGG 0.468552 2_4:+27010669 Msa0323350:CDS
CACGCAGTTTGTTGCAGCGG+CGG 0.472211 2_4:+27009008 Msa0323350:CDS
CATGAACGTCGGTAACGAAC+AGG 0.475779 2_4:-27008819 None:intergenic
ATATATAACATGTTTCTCTT+GGG 0.481990 2_4:-27008880 None:intergenic
TAGAGATTGTCCCTTGATAT+GGG 0.482488 2_4:-27009239 None:intergenic
AAAAGAGTGTGTGTGGGTAA+TGG 0.489755 2_4:-27008500 None:intergenic
TCTCATATCAAGGGAGATGA+TGG 0.490070 2_4:+27011033 Msa0323350:CDS
CACCACGGGTTGACTCGTCT+TGG 0.505317 2_4:-27010661 None:intergenic
TGCCGATCCTCACACGGATG+AGG 0.509768 2_4:+27008576 Msa0323350:CDS
TCTTCAAGGATCCCATATCA+AGG 0.512818 2_4:+27009228 Msa0323350:CDS
AGTTATTTGTTGGGATTCGC+AGG 0.513561 2_4:+27008983 Msa0323350:CDS
AAGCCGCGGGAAGACATTAT+CGG 0.516617 2_4:-27008767 None:intergenic
AGTAATGGAGGCATTGAAGT+TGG 0.517015 2_4:+27009062 Msa0323350:CDS
GACCTCATCCGTGTGAGGAT+CGG 0.528604 2_4:-27008578 None:intergenic
TTTCACAATTCTCATATCAA+GGG 0.529587 2_4:+27011024 Msa0323350:CDS
ACATGTTATATATCTCTGAA+TGG 0.530162 2_4:+27008890 Msa0323350:CDS
AGCGGCGGCGGAGCAGAAGA+AGG 0.532222 2_4:+27009023 Msa0323350:CDS
AAGAGGAAGAAACTCGTGGC+TGG 0.538632 2_4:+27008925 Msa0323350:CDS
GGAGCAGAAGAAGGATGAGT+TGG 0.543711 2_4:+27009032 Msa0323350:CDS
GGTGATACTTTATCATGTAT+AGG 0.550061 2_4:-27010874 None:intergenic
TGGGAAACCCGCGGCAGACA+TGG 0.563883 2_4:-27008861 None:intergenic
AAATTGTTTACTATCCTCGA+GGG 0.568194 2_4:+27009106 Msa0323350:CDS
CGGCACAAAAGCGAGGTATG+TGG 0.571206 2_4:-27008747 None:intergenic
GAAGAAGGATGAGTTGGAGA+AGG 0.573545 2_4:+27009038 Msa0323350:CDS
AGAGTGTGTGTGGGTAATGG+AGG 0.575190 2_4:-27008497 None:intergenic
GAGACGAGGATTCGGCCTCT+AGG 0.578409 2_4:-27008792 None:intergenic
ATTTCTGGACGCACATGCGA+TGG 0.580120 2_4:-27008370 None:intergenic
TCTTGAAAACCCTAAAGAAG+AGG 0.581476 2_4:+27008642 Msa0323350:CDS
TCTCCTTGCCGATCCTCACA+CGG 0.588085 2_4:+27008570 Msa0323350:CDS
CTTCCCGCGGCTTGACCTAG+AGG 0.588102 2_4:+27008777 Msa0323350:CDS
AATGGAGGCATTGAAGTTGG+CGG 0.590740 2_4:+27009065 Msa0323350:CDS
AATGAGATCACTTCAACGGA+AGG 0.592309 2_4:+27010817 Msa0323350:CDS
GAAGTTGGCGGAAGAGAACA+AGG 0.600106 2_4:+27009077 Msa0323350:CDS
GTTCTGATGTTAACAATGCT+CGG 0.602948 2_4:+27008719 Msa0323350:CDS
AAATAACCTCAACCCACCAC+GGG 0.608942 2_4:-27010675 None:intergenic
CTTCAAGGATCCCATATCAA+GGG 0.609531 2_4:+27009229 Msa0323350:CDS
GAGACGATACAAGGAGTGAA+AGG 0.609567 2_4:-27008538 None:intergenic
TTTGTAGAAAAGAGTGTGTG+TGG 0.609647 2_4:-27008507 None:intergenic
GAACACGCAGTTTGTTGCAG+CGG 0.616426 2_4:+27009005 Msa0323350:CDS
TTTGAATGAGATCACTTCAA+CGG 0.618869 2_4:+27010813 Msa0323350:CDS
GTGTTGCAATTGTGAGTCGA+AGG 0.619072 2_4:-27010937 None:intergenic
CGGCCTCTAGGTCAAGCCGC+GGG 0.620797 2_4:-27008780 None:intergenic
CGAAGGAAACTTTGTCTCAG+AGG 0.621557 2_4:-27010920 None:intergenic
TCAACAGCTGAGACGATACA+AGG 0.622390 2_4:-27008547 None:intergenic
GAAATTGTTTACTATCCTCG+AGG 0.624214 2_4:+27009105 Msa0323350:CDS
GTTGGAGAAGGCAGTAATGG+AGG 0.624776 2_4:+27009050 Msa0323350:CDS
ATGAGATCACTTCAACGGAA+GGG 0.630141 2_4:+27010818 Msa0323350:CDS
GTAATGGAGGAAGAAGAAGA+AGG 0.639327 2_4:-27008484 None:intergenic
AGAGTTTATTATTTCCCACA+AGG 0.642714 2_4:+27008442 Msa0323350:CDS
AACGAACAGGTGTTGAGACG+AGG 0.644655 2_4:-27008806 None:intergenic
CAAGACGAGTCAACCCGTGG+TGG 0.644679 2_4:+27010662 Msa0323350:CDS
TTGTGAAAATTGCTTACATG+AGG 0.646619 2_4:-27011009 None:intergenic
AAGACGAGTCAACCCGTGGT+GGG 0.646955 2_4:+27010663 Msa0323350:CDS
TCGGCCTCTAGGTCAAGCCG+CGG 0.648171 2_4:-27008781 None:intergenic
GAAATAACCTCAACCCACCA+CGG 0.649182 2_4:-27010676 None:intergenic
TGGTTGCTTCTGAACAGACA+TGG 0.659994 2_4:-27008350 None:intergenic
TGTTTCTCTTGGGAAACCCG+CGG 0.663878 2_4:-27008870 None:intergenic
ACAAAGACCTCATCCGTGTG+AGG 0.664295 2_4:-27008583 None:intergenic
TTCCAAGACGAGTCAACCCG+TGG 0.669719 2_4:+27010659 Msa0323350:CDS
TTCAAAATAATATGGCTGTG+AGG 0.673161 2_4:-27010895 None:intergenic
ACATTATCGGCACAAAAGCG+AGG 0.676914 2_4:-27008754 None:intergenic
TTGTAGAAAAGAGTGTGTGT+GGG 0.681947 2_4:-27008506 None:intergenic
TGTTAAGAGGAAGAAACTCG+TGG 0.682365 2_4:+27008921 Msa0323350:CDS
GTGCCGATAATGTCTTCCCG+CGG 0.682542 2_4:+27008764 Msa0323350:CDS
TGAAACTTCACCATGAACGT+CGG 0.683050 2_4:-27008830 None:intergenic
AATTGTTTACTATCCTCGAG+GGG 0.689459 2_4:+27009107 Msa0323350:CDS
TGATGGCTATATAAGAGCTG+CGG 0.708137 2_4:+27011050 Msa0323350:CDS
CATCCGTGTGAGGATCGGCA+AGG 0.710355 2_4:-27008573 None:intergenic
TCACACCAATCATCCCCTCG+AGG 0.748749 2_4:-27009120 None:intergenic
CAAAAGAAACAACTTCATCG+CGG 0.749390 2_4:-27008681 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GATTTTCTTAAATTTATTAT+CGG - chr2_4:27009425-27009444 None:intergenic 10.0%
!!! ATAATTTCTTTTTCTTTTCT+TGG + chr2_4:27009302-27009321 Msa0323350:intron 15.0%
!!! TTTTTTTGTGGTATTTTTTT+TGG + chr2_4:27009569-27009588 Msa0323350:intron 15.0%
!!! TATTTTTTTTGGTATAAATC+AGG + chr2_4:27009580-27009599 Msa0323350:intron 15.0%
!! CGTATATTTGAATATTTAAT+TGG + chr2_4:27009921-27009940 Msa0323350:intron 15.0%
!! GTAAAAAAAAATAGATGTTA+AGG - chr2_4:27009968-27009987 None:intergenic 15.0%
!!! CTTTATTTGAACAATTAATA+TGG + chr2_4:27010498-27010517 Msa0323350:intron 15.0%
!! ACGATATTCAAATAAAAAAA+TGG - chr2_4:27010606-27010625 None:intergenic 15.0%
!!! TTTTTTTATTTGAATATCGT+AGG + chr2_4:27010606-27010625 Msa0323350:intron 15.0%
!!! AGTTCTAAATTTTTTTGTTT+GGG - chr2_4:27010728-27010747 None:intergenic 15.0%
!!! TAGTTCTAAATTTTTTTGTT+TGG - chr2_4:27010729-27010748 None:intergenic 15.0%
!! ATATATAACATGTTTCTCTT+GGG - chr2_4:27008883-27008902 None:intergenic 20.0%
!!! GTTGATAAATGATTTTTTTG+TGG + chr2_4:27009557-27009576 Msa0323350:intron 20.0%
!! TCTAATTATAGATATAGCAT+TGG - chr2_4:27009654-27009673 None:intergenic 20.0%
!! TTTAACTATAGATGAAACTT+TGG - chr2_4:27010385-27010404 None:intergenic 20.0%
!! ATAGTTAAATGACTCATTAA+AGG + chr2_4:27010396-27010415 Msa0323350:intron 20.0%
!!! TTCTAAATTTTTTTGTTTGG+GGG - chr2_4:27010726-27010745 None:intergenic 20.0%
!!! GTTCTAAATTTTTTTGTTTG+GGG - chr2_4:27010727-27010746 None:intergenic 20.0%
!! AAATCCATTAACAAAAAGTT+TGG + chr2_4:27011075-27011094 Msa0323350:CDS 20.0%
! GATATATAACATGTTTCTCT+TGG - chr2_4:27008884-27008903 None:intergenic 25.0%
! ACATGTTATATATCTCTGAA+TGG + chr2_4:27008890-27008909 Msa0323350:CDS 25.0%
! TGAGTCTTTCATGAAAATAA+CGG - chr2_4:27008956-27008975 None:intergenic 25.0%
!! TATTTTCATGAAAGACTCAA+CGG + chr2_4:27008957-27008976 Msa0323350:CDS 25.0%
! ATGAATCAATGAAGATTCAA+TGG + chr2_4:27009169-27009188 Msa0323350:CDS 25.0%
! TATCTCGTACTTCTAATTTA+TGG + chr2_4:27009265-27009284 Msa0323350:CDS 25.0%
! AAATGAAATGCAATATCGAT+TGG - chr2_4:27009865-27009884 None:intergenic 25.0%
!! TCGGTTAATTTACCAATTTT+GGG + chr2_4:27010268-27010287 Msa0323350:intron 25.0%
!! GTCATATGAGATAGATTTTA+AGG + chr2_4:27010429-27010448 Msa0323350:intron 25.0%
!!! ATGAGATAGATTTTAAGGTT+AGG + chr2_4:27010434-27010453 Msa0323350:intron 25.0%
!!! TGAGATAGATTTTAAGGTTA+GGG + chr2_4:27010435-27010454 Msa0323350:intron 25.0%
! TTTGAATATCGTAGGTTAAT+TGG + chr2_4:27010614-27010633 Msa0323350:intron 25.0%
! TTGAATATCGTAGGTTAATT+GGG + chr2_4:27010615-27010634 Msa0323350:intron 25.0%
!! TTCCTTTTTATCACTTAGTT+GGG - chr2_4:27010764-27010783 None:intergenic 25.0%
!! TTTCCTTTTTATCACTTAGT+TGG - chr2_4:27010765-27010784 None:intergenic 25.0%
! AACACCAAAAATTATAGTGA+TGG + chr2_4:27010955-27010974 Msa0323350:CDS 25.0%
!! TGAACCATCACTATAATTTT+TGG - chr2_4:27010962-27010981 None:intergenic 25.0%
! GATGGTTCAATTATGTTATT+TGG + chr2_4:27010973-27010992 Msa0323350:CDS 25.0%
!! TTTTCACAATTCTCATATCA+AGG + chr2_4:27011023-27011042 Msa0323350:CDS 25.0%
! TTTCACAATTCTCATATCAA+GGG + chr2_4:27011024-27011043 Msa0323350:CDS 25.0%
!! TACACCAAACTTTTTGTTAA+TGG - chr2_4:27011082-27011101 None:intergenic 25.0%
!!! ATGCAACTTTGGAATTTTGA+CGG - chr2_4:27008419-27008438 None:intergenic 30.0%
TAAACTCTTGAATGCAACTT+TGG - chr2_4:27008430-27008449 None:intergenic 30.0%
AGAGTTTATTATTTCCCACA+AGG + chr2_4:27008442-27008461 Msa0323350:CDS 30.0%
! AATGGAATTCGTTTGTTAAG+AGG + chr2_4:27008908-27008927 Msa0323350:CDS 30.0%
! ATTTTCATGAAAGACTCAAC+GGG + chr2_4:27008958-27008977 Msa0323350:CDS 30.0%
AAATTGTTTACTATCCTCGA+GGG + chr2_4:27009106-27009125 Msa0323350:CDS 30.0%
TCATCTTAACTCTCATTCTA+GGG - chr2_4:27009197-27009216 None:intergenic 30.0%
TTCATCTTAACTCTCATTCT+AGG - chr2_4:27009198-27009217 None:intergenic 30.0%
TGAAGACTGATAAATCTTCA+AGG + chr2_4:27009214-27009233 Msa0323350:CDS 30.0%
GTGTTTCATAGCTAGATAAT+TGG - chr2_4:27009350-27009369 None:intergenic 30.0%
TATTAAACGTCTCCTAATGT+CGG - chr2_4:27009497-27009516 None:intergenic 30.0%
ATACTAGATACTATGAAGCA+CGG - chr2_4:27009528-27009547 None:intergenic 30.0%
CTGTAATAATCTCTCTATGT+GGG - chr2_4:27010040-27010059 None:intergenic 30.0%
TCTGTAATAATCTCTCTATG+TGG - chr2_4:27010041-27010060 None:intergenic 30.0%
!! AAAACAAACGAGGTTTTATG+AGG - chr2_4:27010070-27010089 None:intergenic 30.0%
! TCATAAAACCTCGTTTGTTT+TGG + chr2_4:27010069-27010088 Msa0323350:intron 30.0%
!! AATAAGCATTGATGTCAAAC+GGG - chr2_4:27010110-27010129 None:intergenic 30.0%
!! CAATAAGCATTGATGTCAAA+CGG - chr2_4:27010111-27010130 None:intergenic 30.0%
GTTTGACATCAATGCTTATT+GGG + chr2_4:27010110-27010129 Msa0323350:intron 30.0%
ACATTGAGACTTATCCTATA+GGG - chr2_4:27010215-27010234 None:intergenic 30.0%
TACATTGAGACTTATCCTAT+AGG - chr2_4:27010216-27010235 None:intergenic 30.0%
! GTCGGTTAATTTACCAATTT+TGG + chr2_4:27010267-27010286 Msa0323350:intron 30.0%
TAAATAGCAACTCCCAAAAT+TGG - chr2_4:27010283-27010302 None:intergenic 30.0%
ACAATTAATATGGTGCATTG+TGG + chr2_4:27010508-27010527 Msa0323350:intron 30.0%
!!! AATTTTTTTGTTTGGGGGAA+TGG - chr2_4:27010721-27010740 None:intergenic 30.0%
TTTGAATGAGATCACTTCAA+CGG + chr2_4:27010813-27010832 Msa0323350:CDS 30.0%
GGTGATACTTTATCATGTAT+AGG - chr2_4:27010877-27010896 None:intergenic 30.0%
TTCAAAATAATATGGCTGTG+AGG - chr2_4:27010898-27010917 None:intergenic 30.0%
CAGAGGAGTTCAAAATAATA+TGG - chr2_4:27010906-27010925 None:intergenic 30.0%
TTGTGAAAATTGCTTACATG+AGG - chr2_4:27011012-27011031 None:intergenic 30.0%
! TTGTAGAAAAGAGTGTGTGT+GGG - chr2_4:27008509-27008528 None:intergenic 35.0%
! TTTGTAGAAAAGAGTGTGTG+TGG - chr2_4:27008510-27008529 None:intergenic 35.0%
!!! CTTCTTTAGGGTTTTCAAGA+TGG - chr2_4:27008642-27008661 None:intergenic 35.0%
TCTTGAAAACCCTAAAGAAG+AGG + chr2_4:27008642-27008661 Msa0323350:CDS 35.0%
CAAAAGAAACAACTTCATCG+CGG - chr2_4:27008684-27008703 None:intergenic 35.0%
GTTCTGATGTTAACAATGCT+CGG + chr2_4:27008719-27008738 Msa0323350:CDS 35.0%
TCAACGGGAAAGTTATTTGT+TGG + chr2_4:27008973-27008992 Msa0323350:CDS 35.0%
CAACGGGAAAGTTATTTGTT+GGG + chr2_4:27008974-27008993 Msa0323350:CDS 35.0%
GAAATTGTTTACTATCCTCG+AGG + chr2_4:27009105-27009124 Msa0323350:CDS 35.0%
AATTGTTTACTATCCTCGAG+GGG + chr2_4:27009107-27009126 Msa0323350:CDS 35.0%
! TGATTGGTGTGATTTTGTAG+TGG + chr2_4:27009131-27009150 Msa0323350:CDS 35.0%
TAGAGATTGTCCCTTGATAT+GGG - chr2_4:27009242-27009261 None:intergenic 35.0%
ATAGAGATTGTCCCTTGATA+TGG - chr2_4:27009243-27009262 None:intergenic 35.0%
TTCAGTAGAATCGTATGTGT+CGG + chr2_4:27009451-27009470 Msa0323350:intron 35.0%
! TGCAATATCGATTGGTGTAA+TGG - chr2_4:27009857-27009876 None:intergenic 35.0%
AAATAGATGTTAAGGAGCGA+CGG - chr2_4:27009960-27009979 None:intergenic 35.0%
! TCAAACGGGTATTTTCATTG+TGG - chr2_4:27010096-27010115 None:intergenic 35.0%
CGTTTGACATCAATGCTTAT+TGG + chr2_4:27010109-27010128 Msa0323350:intron 35.0%
TCAAAGAGCAGTAACTAACT+AGG - chr2_4:27010159-27010178 None:intergenic 35.0%
GAGAACAAGTATGTTACTGT+CGG + chr2_4:27010249-27010268 Msa0323350:intron 35.0%
! TTGGGATGAATTTCAAGTCT+CGG + chr2_4:27010633-27010652 Msa0323350:CDS 35.0%
TGCCCAACTAAGTGATAAAA+AGG + chr2_4:27010759-27010778 Msa0323350:CDS 35.0%
!!! AAGAAGGAGAAGCGTTTTCT+AGG - chr2_4:27008471-27008490 None:intergenic 40.0%
! GTAATGGAGGAAGAAGAAGA+AGG - chr2_4:27008487-27008506 None:intergenic 40.0%
! AAAAGAGTGTGTGTGGGTAA+TGG - chr2_4:27008503-27008522 None:intergenic 40.0%
! AGATGGACATCGTTGGTTAT+TGG - chr2_4:27008625-27008644 None:intergenic 40.0%
!! GTTTTCAAGATGGACATCGT+TGG - chr2_4:27008632-27008651 None:intergenic 40.0%
GATTGGCAACCTCTTCTTTA+GGG - chr2_4:27008654-27008673 None:intergenic 40.0%
AGATTGGCAACCTCTTCTTT+AGG - chr2_4:27008655-27008674 None:intergenic 40.0%
TGAAACTTCACCATGAACGT+CGG - chr2_4:27008833-27008852 None:intergenic 40.0%
TGTTAAGAGGAAGAAACTCG+TGG + chr2_4:27008921-27008940 Msa0323350:CDS 40.0%
! AGTTATTTGTTGGGATTCGC+AGG + chr2_4:27008983-27009002 Msa0323350:CDS 40.0%
!! AGTAATGGAGGCATTGAAGT+TGG + chr2_4:27009062-27009081 Msa0323350:CDS 40.0%
!! TGGTGTGATTTTGTAGTGGA+TGG + chr2_4:27009135-27009154 Msa0323350:CDS 40.0%
TCTTCAAGGATCCCATATCA+AGG + chr2_4:27009228-27009247 Msa0323350:CDS 40.0%
CTTCAAGGATCCCATATCAA+GGG + chr2_4:27009229-27009248 Msa0323350:CDS 40.0%
TAATTTATGGCGCATGCTTC+AGG + chr2_4:27009278-27009297 Msa0323350:CDS 40.0%
AACACACTCACAAACACACA+CGG + chr2_4:27009365-27009384 Msa0323350:intron 40.0%
ACATCCTCACTCTTGCAAAA+AGG - chr2_4:27009797-27009816 None:intergenic 40.0%
! TTTTTGCAAGAGTGAGGATG+TGG + chr2_4:27009796-27009815 Msa0323350:intron 40.0%
!! CAAACGAGGTTTTATGAGGA+AGG - chr2_4:27010066-27010085 None:intergenic 40.0%
ATTGTGGACCAAAACAAACG+AGG - chr2_4:27010080-27010099 None:intergenic 40.0%
CTAACTAGGATCGTCTCTAA+TGG - chr2_4:27010145-27010164 None:intergenic 40.0%
TATATTGCTTCGTGCCCTAT+AGG + chr2_4:27010198-27010217 Msa0323350:intron 40.0%
ATATGGTGCATTGTGGCTAA+TGG + chr2_4:27010515-27010534 Msa0323350:intron 40.0%
! TGCATTGTGGCTAATGGTTT+TGG + chr2_4:27010521-27010540 Msa0323350:intron 40.0%
! GCATTGTGGCTAATGGTTTT+GGG + chr2_4:27010522-27010541 Msa0323350:intron 40.0%
AATGAGATCACTTCAACGGA+AGG + chr2_4:27010817-27010836 Msa0323350:CDS 40.0%
ATGAGATCACTTCAACGGAA+GGG + chr2_4:27010818-27010837 Msa0323350:CDS 40.0%
TCTCATATCAAGGGAGATGA+TGG + chr2_4:27011033-27011052 Msa0323350:CDS 40.0%
TGATGGCTATATAAGAGCTG+CGG + chr2_4:27011050-27011069 Msa0323350:CDS 40.0%
GCACATGTGTGCCAAATTTC+TGG - chr2_4:27008388-27008407 None:intergenic 45.0%
!!! CTTTGGAATTTTGACGGCAG+CGG - chr2_4:27008413-27008432 None:intergenic 45.0%
! CGTTTTCTAGGTGACCTTGT+GGG - chr2_4:27008459-27008478 None:intergenic 45.0%
GAGACGATACAAGGAGTGAA+AGG - chr2_4:27008541-27008560 None:intergenic 45.0%
TCAACAGCTGAGACGATACA+AGG - chr2_4:27008550-27008569 None:intergenic 45.0%
TTCATCGCGGTTGTTGAGAT+TGG - chr2_4:27008671-27008690 None:intergenic 45.0%
ACATTATCGGCACAAAAGCG+AGG - chr2_4:27008757-27008776 None:intergenic 45.0%
! AAGTTTTACCATGTCTGCCG+CGG + chr2_4:27008853-27008872 Msa0323350:CDS 45.0%
! GAAGAAGGATGAGTTGGAGA+AGG + chr2_4:27009038-27009057 Msa0323350:CDS 45.0%
! TGAGTTGGAGAAGGCAGTAA+TGG + chr2_4:27009047-27009066 Msa0323350:CDS 45.0%
!! AATGGAGGCATTGAAGTTGG+CGG + chr2_4:27009065-27009084 Msa0323350:CDS 45.0%
TCCTAATGTCGGACACACAT+CGG - chr2_4:27009486-27009505 None:intergenic 45.0%
CCTCACTCTTGCAAAAAGGT+AGG - chr2_4:27009793-27009812 None:intergenic 45.0%
! CCTACCTTTTTGCAAGAGTG+AGG + chr2_4:27009790-27009809 Msa0323350:intron 45.0%
AAATAACCTCAACCCACCAC+GGG - chr2_4:27010678-27010697 None:intergenic 45.0%
GAAATAACCTCAACCCACCA+CGG - chr2_4:27010679-27010698 None:intergenic 45.0%
CGAAGGAAACTTTGTCTCAG+AGG - chr2_4:27010923-27010942 None:intergenic 45.0%
GTGTTGCAATTGTGAGTCGA+AGG - chr2_4:27010940-27010959 None:intergenic 45.0%
!!! ATATTTTAATATATCAATAA+TGG + chr2_4:27010010-27010029 Msa0323350:intron 5.0%
!!! TATTTTAATATATCAATAAT+GGG + chr2_4:27010011-27010030 Msa0323350:intron 5.0%
! ATTTCTGGACGCACATGCGA+TGG - chr2_4:27008373-27008392 None:intergenic 50.0%
CGCATGTGCGTCCAGAAATT+TGG + chr2_4:27008374-27008393 Msa0323350:CDS 50.0%
! GCGTTTTCTAGGTGACCTTG+TGG - chr2_4:27008460-27008479 None:intergenic 50.0%
! AGAGTGTGTGTGGGTAATGG+AGG - chr2_4:27008500-27008519 None:intergenic 50.0%
ACAAAGACCTCATCCGTGTG+AGG - chr2_4:27008586-27008605 None:intergenic 50.0%
AAGCCGCGGGAAGACATTAT+CGG - chr2_4:27008770-27008789 None:intergenic 50.0%
!! CAGGTGTTGAGACGAGGATT+CGG - chr2_4:27008803-27008822 None:intergenic 50.0%
!! AACGAACAGGTGTTGAGACG+AGG - chr2_4:27008809-27008828 None:intergenic 50.0%
CATGAACGTCGGTAACGAAC+AGG - chr2_4:27008822-27008841 None:intergenic 50.0%
CTGTTCGTTACCGACGTTCA+TGG + chr2_4:27008820-27008839 Msa0323350:CDS 50.0%
! AGTTTTACCATGTCTGCCGC+GGG + chr2_4:27008854-27008873 Msa0323350:CDS 50.0%
TGTTTCTCTTGGGAAACCCG+CGG - chr2_4:27008873-27008892 None:intergenic 50.0%
AAGAGGAAGAAACTCGTGGC+TGG + chr2_4:27008925-27008944 Msa0323350:CDS 50.0%
!! GAACACGCAGTTTGTTGCAG+CGG + chr2_4:27009005-27009024 Msa0323350:CDS 50.0%
GGAGCAGAAGAAGGATGAGT+TGG + chr2_4:27009032-27009051 Msa0323350:CDS 50.0%
GTTGGAGAAGGCAGTAATGG+AGG + chr2_4:27009050-27009069 Msa0323350:CDS 50.0%
GAAGTTGGCGGAAGAGAACA+AGG + chr2_4:27009077-27009096 Msa0323350:CDS 50.0%
ACTATCCTCGAGGGGATGAT+TGG + chr2_4:27009115-27009134 Msa0323350:CDS 50.0%
ACCGATGTGTGTCCGACATT+AGG + chr2_4:27009482-27009501 Msa0323350:intron 50.0%
TTTGTTTGGGGGAATGGCGT+CGG - chr2_4:27010715-27010734 None:intergenic 50.0%
TCTCCTTGCCGATCCTCACA+CGG + chr2_4:27008570-27008589 Msa0323350:CDS 55.0%
GACCTCATCCGTGTGAGGAT+CGG - chr2_4:27008581-27008600 None:intergenic 55.0%
CGGCACAAAAGCGAGGTATG+TGG - chr2_4:27008750-27008769 None:intergenic 55.0%
! GTGCCGATAATGTCTTCCCG+CGG + chr2_4:27008764-27008783 Msa0323350:CDS 55.0%
TCACACCAATCATCCCCTCG+AGG - chr2_4:27009123-27009142 None:intergenic 55.0%
TTCCAAGACGAGTCAACCCG+TGG + chr2_4:27010659-27010678 Msa0323350:CDS 55.0%
AAGACGAGTCAACCCGTGGT+GGG + chr2_4:27010663-27010682 Msa0323350:CDS 55.0%
CATCCGTGTGAGGATCGGCA+AGG - chr2_4:27008576-27008595 None:intergenic 60.0%
TGCCGATCCTCACACGGATG+AGG + chr2_4:27008576-27008595 Msa0323350:CDS 60.0%
GAGACGAGGATTCGGCCTCT+AGG - chr2_4:27008795-27008814 None:intergenic 60.0%
!! CACGCAGTTTGTTGCAGCGG+CGG + chr2_4:27009008-27009027 Msa0323350:CDS 60.0%
!! CACCACGGGTTGACTCGTCT+TGG - chr2_4:27010664-27010683 None:intergenic 60.0%
CAAGACGAGTCAACCCGTGG+TGG + chr2_4:27010662-27010681 Msa0323350:CDS 60.0%
!! AGTCAACCCGTGGTGGGTTG+AGG + chr2_4:27010669-27010688 Msa0323350:CDS 60.0%
CTTCCCGCGGCTTGACCTAG+AGG + chr2_4:27008777-27008796 Msa0323350:CDS 65.0%
TCGGCCTCTAGGTCAAGCCG+CGG - chr2_4:27008784-27008803 None:intergenic 65.0%
TGGGAAACCCGCGGCAGACA+TGG - chr2_4:27008864-27008883 None:intergenic 65.0%
!! GCAGTTTGTTGCAGCGGCGG+CGG + chr2_4:27009011-27009030 Msa0323350:CDS 65.0%
CGGCCTCTAGGTCAAGCCGC+GGG - chr2_4:27008783-27008802 None:intergenic 70.0%
AGCGGCGGCGGAGCAGAAGA+AGG + chr2_4:27009023-27009042 Msa0323350:CDS 70.0%
! TGGGGGAATGGCGTCGGAGC+AGG - chr2_4:27010709-27010728 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 27008352 27011110 27008352 ID=Msa0323350;Name=Msa0323350
chr2_4 mRNA 27008352 27011110 27008352 ID=Msa0323350-mRNA-1;Parent=Msa0323350;Name=Msa0323350-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|0|0|1|1|1|2|0|476
chr2_4 exon 27008352 27009299 27008352 ID=Msa0323350-mRNA-1:exon:9446;Parent=Msa0323350-mRNA-1
chr2_4 exon 27010628 27011110 27010628 ID=Msa0323350-mRNA-1:exon:9447;Parent=Msa0323350-mRNA-1
chr2_4 CDS 27008352 27009299 27008352 ID=Msa0323350-mRNA-1:cds;Parent=Msa0323350-mRNA-1
chr2_4 CDS 27010628 27011110 27010628 ID=Msa0323350-mRNA-1:cds;Parent=Msa0323350-mRNA-1
Gene Sequence

>Msa0323350

ATGTCTGTTCAGAAGCAACCATCGCATGTGCGTCCAGAAATTTGGCACACATGTGCTGCCGCTGCCGTCAAAATTCCAAAGTTGCATTCAAGAGTTTATTATTTCCCACAAGGTCACCTAGAAAACGCTTCTCCTTCTTCTTCTTCCTCCATTACCCACACACACTCTTTTCTACAAAGTTTTCGTCCTTTCACTCCTTGTATCGTCTCAGCTGTTGATCTCCTTGCCGATCCTCACACGGATGAGGTCTTTGTTAAACTGCTACTTACTCCAATAACCAACGATGTCCATCTTGAAAACCCTAAAGAAGAGGTTGCCAATCTCAACAACCGCGATGAAGTTGTTTCTTTTGTCAAGATTCTCACTCGTTCTGATGTTAACAATGCTCGGTCATTCCACATACCTCGCTTTTGTGCCGATAATGTCTTCCCGCGGCTTGACCTAGAGGCCGAATCCTCGTCTCAACACCTGTTCGTTACCGACGTTCATGGTGAAGTTTCAAAGTTTTACCATGTCTGCCGCGGGTTTCCCAAGAGAAACATGTTATATATCTCTGAATGGAATTCGTTTGTTAAGAGGAAGAAACTCGTGGCTGGAGATTCCGTTATTTTCATGAAAGACTCAACGGGAAAGTTATTTGTTGGGATTCGCAGGAACACGCAGTTTGTTGCAGCGGCGGCGGAGCAGAAGAAGGATGAGTTGGAGAAGGCAGTAATGGAGGCATTGAAGTTGGCGGAAGAGAACAAGGCATTTGAAATTGTTTACTATCCTCGAGGGGATGATTGGTGTGATTTTGTAGTGGATGGTAATGCTGTTGATGAATCAATGAAGATTCAATGGAACCCTAGAATGAGAGTTAAGATGAAGACTGATAAATCTTCAAGGATCCCATATCAAGGGACAATCTCTATTGTATCTCGTACTTCTAATTTATGGCGCATGCTTCAGGTTAATTGGGATGAATTTCAAGTCTCGGAAATTCCAAGACGAGTCAACCCGTGGTGGGTTGAGGTTATTTCTCACAAACCTGCTCCGACGCCATTCCCCCAAACAAAAAAATTTAGAACTACTCAAAGTTCTGCCCAACTAAGTGATAAAAAGGAAACATTATTGAATGATGATGCTTCAAACATTTTGAATGAGATCACTTCAACGGAAGGGTTAAGTAATGTGTCAAAAGAATTAAACATGAGTCCTATACATGATAAAGTATCACCTCACAGCCATATTATTTTGAACTCCTCTGAGACAAAGTTTCCTTCGACTCACAATTGCAACACCAAAAATTATAGTGATGGTTCAATTATGTTATTTGGTAAGATCATACAGCCTCATGTAAGCAATTTTCACAATTCTCATATCAAGGGAGATGATGGCTATATAAGAGCTGCGGTGAAATCCATTAACAAAAAGTTTGGTGTAAAGAACTAG
Protein sequence

>Msa0323350

MSVQKQPSHVRPEIWHTCAAAAVKIPKLHSRVYYFPQGHLENASPSSSSSITHTHSFLQSFRPFTPCIVSAVDLLADPHTDEVFVKLLLTPITNDVHLENPKEEVANLNNRDEVVSFVKILTRSDVNNARSFHIPRFCADNVFPRLDLEAESSSQHLFVTDVHGEVSKFYHVCRGFPKRNMLYISEWNSFVKRKKLVAGDSVIFMKDSTGKLFVGIRRNTQFVAAAAEQKKDELEKAVMEALKLAEENKAFEIVYYPRGDDWCDFVVDGNAVDESMKIQWNPRMRVKMKTDKSSRIPYQGTISIVSRTSNLWRMLQVNWDEFQVSEIPRRVNPWWVEVISHKPAPTPFPQTKKFRTTQSSAQLSDKKETLLNDDASNILNEITSTEGLSNVSKELNMSPIHDKVSPHSHIILNSSETKFPSTHNCNTKNYSDGSIMLFGKIIQPHVSNFHNSHIKGDDGYIRAAVKSINKKFGVKN*