AlfalfaGEDB | Msa0324510

M. sativa cultivar zhongmu-4 / Msa0324510

KEGG: K04733
GO: GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0324510	XP_013463930.1	96.681	452	9	1	1	446	1	452	0.0	895

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0324510	sp\|Q94C25\|Y5005_ARATH	64.428	402	139	3	1	400	1	400	6.03e-175	500

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0324510	MtrunA17_Chr2g0306811	96.681	452	9	1	1	446	1	452	0.0	895
Msa0324510	MtrunA17_Chr4g0063251	72.566	452	111	4	1	446	1	445	0.0	661
Msa0324510	MtrunA17_Chr3g0137741	45.070	355	172	7	51	403	444	777	2.44e-101	320
Msa0324510	MtrunA17_Chr1g0149381	42.469	405	203	9	44	445	446	823	1.55e-98	313
Msa0324510	MtrunA17_Chr2g0318171	44.828	348	171	7	51	398	436	762	7.65e-98	310
Msa0324510	MtrunA17_Chr2g0314611	45.087	346	169	7	53	398	442	766	1.30e-96	307
Msa0324510	MtrunA17_Chr2g0333091	43.417	357	182	6	42	398	436	772	8.01e-93	298
Msa0324510	MtrunA17_Chr6g0467301	45.122	328	163	7	80	404	469	782	3.14e-91	294
Msa0324510	MtrunA17_Chr1g0202331	41.892	370	183	9	42	404	478	822	1.62e-90	293
Msa0324510	MtrunA17_Chr6g0467281	43.333	330	172	6	76	403	460	776	3.20e-89	288

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0324510	AT5G20050.1	64.428	402	139	3	1	400	1	400	6.13e-176	500
Msa0324510	AT4G32300.1	43.655	394	191	9	57	445	452	819	1.50e-102	324
Msa0324510	AT5G24080.3	47.319	317	150	6	90	404	390	691	3.53e-90	290
Msa0324510	AT5G24080.1	47.319	317	150	6	90	404	390	691	3.53e-90	290
Msa0324510	AT5G24080.2	47.319	317	150	6	90	404	517	818	2.11e-89	290
Msa0324510	AT1G34300.1	45.000	320	161	5	79	397	460	765	2.54e-89	289
Msa0324510	AT2G19130.1	45.152	330	167	5	84	412	474	790	4.65e-85	278
Msa0324510	AT4G00340.2	47.682	302	142	7	93	390	451	740	5.55e-85	277
Msa0324510	AT4G00340.1	47.682	302	142	7	93	390	472	761	7.60e-85	277
Msa0324510	AT5G35370.1	40.148	406	195	9	22	408	444	820	3.74e-82	271

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Find 83 sgRNAs with CRISPR-Local

Find 204 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTGATGTATATAGTTA+CGG	0.435657	2_4:+30171058	Msa0324510:CDS
AAACAAAACCAAATACTGCA+AGG	0.630788	2_4:-30170322	None:intergenic
AAACTGATGTATATAGTTAC+GGG	0.439882	2_4:+30171059	Msa0324510:CDS
AAAGCCAGAAAATATTCTCT+TGG	0.452095	2_4:+30170895	Msa0324510:CDS
AACAGTAGTAGCAAAAGAGT+AGG	0.608755	2_4:-30171491	None:intergenic
AAGAGGAACAAGAGGTTATA+TGG	0.381755	2_4:+30171000	Msa0324510:CDS
AAGGCTTTCTTCCTCATCTG+TGG	0.511857	2_4:+30170281	Msa0324510:CDS
AATAAGTGATTGAAATCCAT+CGG	0.546856	2_4:-30170492	None:intergenic
AATGTGGTGGTGTTGATGAA+AGG	0.590144	2_4:+30171239	Msa0324510:CDS
AATTGGTTTATGTTGCTTTA+TGG	0.196132	2_4:+30171272	Msa0324510:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAACAAAACCAAATACTGCA+AGG	-	chr2_4:30170325-30170344	None:intergenic	30.0%
AGTTTCTTAAGAAAAGTTGC+AGG	+	chr2_4:30170428-30170447	Msa0324510:CDS	30.0%
GTTTCTTAAGAAAAGTTGCA+GGG	+	chr2_4:30170429-30170448	Msa0324510:CDS	30.0%
TTTCTTAAGAAAAGTTGCAG+GGG	+	chr2_4:30170430-30170449	Msa0324510:CDS	30.0%
TCATTGTATCTGAACTTTGT+TGG	-	chr2_4:30170458-30170477	None:intergenic	30.0%
TTTCAATCACTTATTGGAAG+AGG	+	chr2_4:30170500-30170519	Msa0324510:CDS	30.0%
CAAATGGATCATTAGATTGT+TGG	+	chr2_4:30170726-30170745	Msa0324510:CDS	30.0%
AAAGCCAGAAAATATTCTCT+TGG	+	chr2_4:30170895-30170914	Msa0324510:CDS	30.0%
GTGTTTAGTTGAAGATGAAA+AGG	+	chr2_4:30171120-30171139	Msa0324510:CDS	30.0%
ATGAAAAGGACAAGTCAAAA+AGG	+	chr2_4:30171134-30171153	Msa0324510:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_4	gene	30170188	30171528	30170188	ID=Msa0324510;Name=Msa0324510
chr2_4	mRNA	30170188	30171528	30170188	ID=Msa0324510-mRNA-1;Parent=Msa0324510;Name=Msa0324510-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|446
chr2_4	exon	30170188	30171528	30170188	ID=Msa0324510-mRNA-1:exon:10137;Parent=Msa0324510-mRNA-1
chr2_4	CDS	30170188	30171528	30170188	ID=Msa0324510-mRNA-1:cds;Parent=Msa0324510-mRNA-1

Gene Sequence

>Msa0324510

ATGGAAGATAGGAAAGTGAATATAATCGCTGCTATATCAGTGATAACACTGATCATCATCATAATCATTGCACGTATCTTGTTAAAGCTTTCAAAGGCTTTCTTCCTCATCTGTGGTGCTGGCGTTGCTGTGATCCTTGCAGTATTTGGTTTTGTTTTTCTCAGCTTAATCTACAAACGCAGAAGGAGAATATTGGAATCACAGTTGAAATCAGAAGGTCAAGAGATTCGAATAGAGTACAGTTTCTTAAGAAAAGTTGCAGGGGTTCCAACAAAGTTCAGATACAATGAGCTTGAGGAAGCAACCGATGGATTTCAATCACTTATTGGAAGAGGTTCTTCGGCTTCGGTTTTCAAAGGCATTCTCAACGATGGAACTTCGGTTGCTGTGAAAAGAATTGATGCAGAAGAAAGAGGAGCAAGAGAATTTAAATCAGAAGTTGCAGCTATAGCTAATGTTCATCATGTGAATCTAGTGAGACTTTTTGGGTATTGTAATTCTTCTTCAGCTCCTAGGTACCTTGTTTATGATTATATATCAAATGGATCATTAGATTGTTGGATTTTCCCGAAAAGGGATTCGCAAAGGCGTCCACGTCCTGGTGGATGCTTGTCGTGGAACTTGAGGTATAAAGTCGCAATTGACGTTGCTAAAGGACTTGCATATCTTCACCACGATTGCAGATCAAGGATCTTACATCTTGATATAAAGCCAGAAAATATTCTCTTGGATGAGACTTTTCGAGCACTTGTTTCGGATTTCGGTCTTGCAAAGCTAACTGGTAAAGACGAAAGTCAAGCGGTGTCTACGATAAGAGGAACAAGAGGTTATATGGCACCTGAATGGCTTTTAGAAAAGGGTATTTCAGATAAAACTGATGTATATAGTTACGGGATGGTTTTATTAGAAATTGTTGGAGGAAGAAAAAATGTGTGTTTAGTTGAAGATGAAAAGGACAAGTCAAAAAGGAAATGGCAATATTTTCCAAAGATTGTAAATGAAAAAGTGAAACAAGGGAAAATCATGGAAATTGTTGATCATAGGTTAATGGAATGTGGTGGTGTTGATGAAAGGGAGGTGGTTAAATTGGTTTATGTTGCTTTATGGTGTGTGCAAGAGAAGCCAAGGTTGAGGCCTAGTATGGGTAGAGTGGTTGATATGCTTGAAGGGCGTGTGAGAGTGGATGAGCCACCAGGTACAAGAATGATTCTTGTTGATTTTCTAAGTGTTGATGATGATAACATGCCAAGGTTGGACCCTATGTCTAGTCAAGGAACACAAAGCGATGTGGAATGTAATTCAACCTACTCTTTTGCTACTACTGTTTTCTCTGGAAGATAG

Protein sequence

>Msa0324510

MEDRKVNIIAAISVITLIIIIIIARILLKLSKAFFLICGAGVAVILAVFGFVFLSLIYKRRRRILESQLKSEGQEIRIEYSFLRKVAGVPTKFRYNELEEATDGFQSLIGRGSSASVFKGILNDGTSVAVKRIDAEERGAREFKSEVAAIANVHHVNLVRLFGYCNSSSAPRYLVYDYISNGSLDCWIFPKRDSQRRPRPGGCLSWNLRYKVAIDVAKGLAYLHHDCRSRILHLDIKPENILLDETFRALVSDFGLAKLTGKDESQAVSTIRGTRGYMAPEWLLEKGISDKTDVYSYGMVLLEIVGGRKNVCLVEDEKDKSKRKWQYFPKIVNEKVKQGKIMEIVDHRLMECGGVDEREVVKLVYVALWCVQEKPRLRPSMGRVVDMLEGRVRVDEPPGTRMILVDFLSVDDDNMPRLDPMSSQGTQSDVECNSTYSFATTVFSGR*