AlfalfaGEDB | Msa0327840

M. sativa cultivar zhongmu-4 / Msa0327840

KEGG: -
GO: GO:0000003 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0009653 GO:0009826 GO:0009856 GO:0009860 GO:0009888 GO:0009932 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0016020 GO:0016043 GO:0016049 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0021700 GO:0022414 GO:0022622 GO:0023052 GO:0030154 GO:0032501 GO:0032502 GO:0032989 GO:0035556 GO:0036211 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0044706 GO:0048364 GO:0048468 GO:0048469 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051716 GO:0060560 GO:0065007 GO:0071695 GO:0071704 GO:0071840 GO:0071944 GO:0080147 GO:0090558 GO:0090627 GO:0099402 GO:0140096 GO:1901564 GO:1905392
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0327840	XP_003595879.3	98.057	566	10	1	2	566	6	571	0.0	1152

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0327840	sp\|Q1PFB9\|AGC17_ARATH	72.160	449	104	9	136	566	110	555	0.0	647

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0327840	MtrunA17_Chr2g0309421	98.057	566	10	1	2	566	6	571	0.0	1152
Msa0327840	MtrunA17_Chr8g0356291	66.160	526	163	9	48	566	7	524	0.0	677
Msa0327840	MtrunA17_Chr2g0290691	62.528	451	155	8	126	566	158	604	0.0	563
Msa0327840	MtrunA17_Chr8g0335351	68.146	383	107	5	146	520	345	720	0.0	538
Msa0327840	MtrunA17_Chr7g0250631	59.488	469	145	10	132	566	304	761	0.0	536
Msa0327840	MtrunA17_Chr7g0227331	54.781	502	185	9	90	566	134	618	3.01e-178	517
Msa0327840	MtrunA17_Chr6g0449401	53.388	487	162	8	122	566	216	679	6.82e-171	500
Msa0327840	MtrunA17_Chr1g0179101	57.377	427	164	6	146	566	445	859	2.25e-164	489
Msa0327840	MtrunA17_Chr7g0266031	60.660	394	148	5	136	526	459	848	3.70e-164	489
Msa0327840	MtrunA17_Chr3g0080101	59.080	413	147	7	138	535	382	787	1.26e-159	475

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
Msa0327840	AT1G79250.1	72.160	449	104	9	136	566	110	555	647
Msa0327840	AT1G79250.3	72.160	449	104	9	136	566	110	555	647
Msa0327840	AT1G79250.2	72.160	449	104	9	136	566	110	555	647
Msa0327840	AT1G79250.4	72.160	449	104	9	136	566	110	555	647
Msa0327840	AT3G12690.1	71.395	430	106	5	145	566	157	577	627
Msa0327840	AT3G12690.3	71.395	430	106	5	145	566	157	577	627
Msa0327840	AT3G12690.2	71.395	430	106	5	145	566	157	577	627
Msa0327840	AT1G16440.1	70.023	427	109	8	146	566	86	499	607
Msa0327840	AT5G55910.2	59.283	474	163	7	110	566	38	498	546
Msa0327840	AT5G55910.1	59.283	474	163	7	110	566	38	498	546

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Find 98 sgRNAs with CRISPR-Local

Find 284 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATCAGACTTCGGCTTAT+TGG	0.212196	2_4:+39799975	Msa0327840:CDS
AAACCGCCTTCCTGAACTAA+TGG	0.437581	2_4:+39800005	Msa0327840:CDS
AAATGACATTGATCTTACAT+TGG	0.536629	2_4:-39800037	None:intergenic
AACATTAGGCCACATACTGG+TGG	0.650784	2_4:+39798820	Msa0327840:CDS
AACTTCTTATCAACTGGTGG+TGG	0.533626	2_4:-39800453	None:intergenic
AAGAGAATTGCATATAAAAG+AGG	0.501521	2_4:+39800306	Msa0327840:CDS
AAGGTGGGATGCAATTAACA+TGG	0.670858	2_4:+39798849	Msa0327840:CDS
AAGTCGTCCTCCACCCACGG+AGG	0.747358	2_4:+39799835	Msa0327840:CDS
AATACAAAGACGAACAATCA+AGG	0.539318	2_4:+39798736	Msa0327840:CDS
AATCAAGGTGCGCCAGGAAG+TGG	0.462571	2_4:+39798751	Msa0327840:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATACAAAGACGAACAATCA+AGG	+	chr2_4:39798736-39798755	Msa0327840:CDS	30.0%
CAAGTTGTTCATCAAACATT+AGG	+	chr2_4:39798806-39798825	Msa0327840:CDS	30.0%
ATTAACATGGCTTCCAAAAA+TGG	+	chr2_4:39798862-39798881	Msa0327840:CDS	30.0%
CTAAAATGGGTAAGATTTAG+AGG	-	chr2_4:39798889-39798908	None:intergenic	30.0%
TTTCATAGCAAAATGAGCAT+CGG	-	chr2_4:39798974-39798993	None:intergenic	30.0%
TTCTTAGATCACAAACTGAA+AGG	+	chr2_4:39799031-39799050	Msa0327840:CDS	30.0%
TCTTAGATCACAAACTGAAA+GGG	+	chr2_4:39799032-39799051	Msa0327840:CDS	30.0%
GAATACAAAGTAGGCAAAAA+GGG	-	chr2_4:39799081-39799100	None:intergenic	30.0%
AGAATACAAAGTAGGCAAAA+AGG	-	chr2_4:39799082-39799101	None:intergenic	30.0%
TCTTCAGTGAAACACTTATT+TGG	-	chr2_4:39799186-39799205	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_4	gene	39798391	39800548	39798391	ID=Msa0327840;Name=Msa0327840
chr2_4	mRNA	39798391	39800548	39798391	ID=Msa0327840-mRNA-1;Parent=Msa0327840;Name=Msa0327840-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0\|0\|0\|1\|1\|1\|2\|0\|566
chr2_4	exon	39798391	39799214	39798391	ID=Msa0327840-mRNA-1:exon:12039;Parent=Msa0327840-mRNA-1
chr2_4	exon	39799672	39800548	39799672	ID=Msa0327840-mRNA-1:exon:12040;Parent=Msa0327840-mRNA-1
chr2_4	CDS	39798391	39799214	39798391	ID=Msa0327840-mRNA-1:cds;Parent=Msa0327840-mRNA-1
chr2_4	CDS	39799672	39800548	39799672	ID=Msa0327840-mRNA-1:cds;Parent=Msa0327840-mRNA-1

Gene Sequence

>Msa0327840

ATGCAGATGAATCACAATACATCAGAAGAACATTTTGAGACACATCATAATGCTCGTAGTTATCCGAATTCAGGAAACAATTCACCAACTCATCACAAAAAGTTTAATAATGAAGAAAAAGAAGCATACAAAAATGAATATTCAGCAAGTGAATCTACTAAAAATATAGACCAAGTTTCTAAAACAGATTCAAATTCTGCAAATAAAACTAGACCACAGAAACTAAGTCCACCAAACAAAAACATTCCTGATGCTTTCTTAAGACAAAGTCCAAGGAACAGGATGCCTTCAAATTCTGATGCTGCAACTCCATTACCATCAGGAGTTTCAAAGGGTATGTCGACTAATACAAAGACGAACAATCAAGGTGCGCCAGGAAGTGGAGCTAGCTCGCGAAGCGACAGTTTAGAGAGCACAAGTTGTTCATCAAACATTAGGCCACATACTGGTGGTGATATAAGGTGGGATGCAATTAACATGGCTTCCAAAAATGGTCCTCTAAATCTTACCCATTTTAGGCTTCTGAAGCGTATCGGTTATGGAGACATTGGAAGTGTTTATCTTGTTGAACTTAAAGGAACCGATGCTCATTTTGCTATGAAAGTAATGGACAAGGCTGCTCTTATAAGTAGAAACAAACTTCTTAGATCACAAACTGAAAGGGAGATTCTTGGACTTCTTGATCATCCCTTTTTGCCTACTTTGTATTCTTACTTTGAGACTGATAAGTTTTACTGTCTGGTTATGGAGTACTGTAGTGGCGGCGATCTTCATTCGCTTCGACAGAAACAACCAAATAAGTGTTTCACTGAAGAAGCTGCAAGGTTTTATGCTTCCGAGGTTTTGTTAGCTCTTGAATACCTACACATGCTTGGCATTGTATACCGAGACTTAAAGCCAGAAAATCTTTTAGTTAGGGACGAAGGTCACATCATGCTCTCAGATTTCGATTTATCGCTTCGCTGTTCTGTCTGTCCAACACTTGTCAAGTCGTCCTCCACCCACGGAGGAAACAGCAGCGGCAATAGTGATTCCGGAGGTATATTGAACGATGACCAAGCAGTGATTGCTCAGTCATCAACAAGCTTCTTCCCTCGCATTTTACCTTCAAAGAAAAATCGAAAAGCAAAATCAGACTTCGGCTTATTGGTTAACGGAAACCGCCTTCCTGAACTAATGGCAGAACCAACCAATGTAAGATCAATGTCATTTGTAGGGACACACGAGTATCTCGCACCTGAAATCATCAAAGGAGAAGGACACGGTAGCGCGGTAGATTGGTGGACTTTCGGTATCTTCTTGTATGAATTGTTGCACGGTGCAACGCCTTTTAAAGGCGCAGGATACAAAGCAACACTTTTCAATGTTGTTGGACAGCCATTAAGGTTTCCAGATAGTCCTCAAATTAGTCCCGTGGCTAGAGATCTTATTAGAGGATTGTTGATTAAGGAACCTCAGAAGAGAATTGCATATAAAAGAGGAGCAACAGAAATTAAACAGCATCCATTTTTTGAAGGAGTGAATTGGGCTCTTGTTAGAAGTGCAACACCACCACATATACCAGATGCTATAGACTTTTCAAAGTATGCTAAAAAAGAAACAGCACCACCACCAGTTGATAAGAAGTTGCCAGATGTTACCAATGATAAAAAAGGCAAAACTTCAACTGATAATTCTTATGTAGATTTTGAGTATTTTTAG

Protein sequence

>Msa0327840

MQMNHNTSEEHFETHHNARSYPNSGNNSPTHHKKFNNEEKEAYKNEYSASESTKNIDQVSKTDSNSANKTRPQKLSPPNKNIPDAFLRQSPRNRMPSNSDAATPLPSGVSKGMSTNTKTNNQGAPGSGASSRSDSLESTSCSSNIRPHTGGDIRWDAINMASKNGPLNLTHFRLLKRIGYGDIGSVYLVELKGTDAHFAMKVMDKAALISRNKLLRSQTEREILGLLDHPFLPTLYSYFETDKFYCLVMEYCSGGDLHSLRQKQPNKCFTEEAARFYASEVLLALEYLHMLGIVYRDLKPENLLVRDEGHIMLSDFDLSLRCSVCPTLVKSSSTHGGNSSGNSDSGGILNDDQAVIAQSSTSFFPRILPSKKNRKAKSDFGLLVNGNRLPELMAEPTNVRSMSFVGTHEYLAPEIIKGEGHGSAVDWWTFGIFLYELLHGATPFKGAGYKATLFNVVGQPLRFPDSPQISPVARDLIRGLLIKEPQKRIAYKRGATEIKQHPFFEGVNWALVRSATPPHIPDAIDFSKYAKKETAPPPVDKKLPDVTNDKKGKTSTDNSYVDFEYF*