AlfalfaGEDB | Msa0331130

M. sativa cultivar zhongmu-4 / Msa0331130

KEGG: -
GO: -
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0331130	XP_013464450.2	98.592	355	5	0	74	428	1	355	0.0	727

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0331130	sp\|Q9SYM9\|Y1853_ARATH	74.041	339	79	3	84	417	11	345	0.0	530

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0331130	MtrunA17_Chr2g0313911	98.592	355	5	0	74	428	1	355	0.0	727
Msa0331130	MtrunA17_Chr5g0409041	45.029	342	180	4	78	417	239	574	4.07e-96	300
Msa0331130	MtrunA17_Chr4g0060011	41.761	352	194	5	73	418	240	586	1.22e-90	286
Msa0331130	MtrunA17_Chr2g0299301	46.844	301	152	3	125	417	1034	1334	1.02e-79	268
Msa0331130	MtrunA17_Chr2g0317071	42.540	315	181	0	108	422	757	1071	6.56e-79	264
Msa0331130	MtrunA17_Chr3g0079661	46.441	295	155	3	130	422	901	1194	1.07e-78	265
Msa0331130	MtrunA17_Chr8g0345561	45.051	293	161	0	131	423	795	1087	3.76e-78	262
Msa0331130	MtrunA17_Chr1g0203631	43.894	303	168	2	121	422	970	1271	8.83e-78	262
Msa0331130	MtrunA17_Chr1g0200911	44.326	282	148	3	142	417	301	579	3.00e-76	249
Msa0331130	MtrunA17_Chr7g0262521	45.517	290	156	2	130	417	331	620	1.02e-75	250

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0331130	AT1G78530.1	74.041	339	79	3	84	417	11	345	0.0	530
Msa0331130	AT1G78530.2	78.014	282	55	2	84	362	11	288	7.79e-167	470
Msa0331130	AT1G31420.2	46.491	342	175	4	78	417	239	574	9.88e-101	312
Msa0331130	AT1G31420.1	46.199	342	177	4	78	417	239	575	1.60e-99	309
Msa0331130	AT1G31420.3	46.199	342	177	4	78	417	239	575	1.60e-99	309
Msa0331130	AT2G35620.3	46.667	345	174	5	76	417	235	572	2.08e-98	306
Msa0331130	AT2G35620.1	46.667	345	174	5	76	417	235	572	2.08e-98	306
Msa0331130	AT2G35620.2	46.667	345	174	5	76	417	235	572	2.08e-98	306
Msa0331130	AT2G35620.4	46.667	345	174	5	76	417	235	572	2.08e-98	306
Msa0331130	AT5G62710.2	43.526	363	190	5	65	418	194	550	6.92e-98	304

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Find 103 sgRNAs with CRISPR-Local

Find 225 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATCTTTAACAACTGATA+TGG	0.371052	2_4:-46514937	Msa0331130:CDS
AAAGACATTATTGGTTCTGG+TGG	0.556268	2_4:-46514885	Msa0331130:CDS
AAAGCTATTCTTTGTCTTGT+TGG	0.306823	2_4:+46514254	None:intergenic
AAAGGGGATGTTTACAGTTT+TGG	0.233200	2_4:-46513919	Msa0331130:CDS
AACAAAGACATTATTGGTTC+TGG	0.405916	2_4:-46514888	Msa0331130:CDS
AACGGACCTCTGCAAGTGGA+CGG	0.616814	2_4:-46517017	Msa0331130:intron
AAGCATATGATTTATGAAGA+AGG	0.478953	2_4:-46515485	Msa0331130:CDS
AAGGAATCCAAACTTCCATT+AGG	0.533517	2_4:+46514663	None:intergenic
ACAAGACAAAGAATAGCTTT+AGG	0.463698	2_4:-46514251	Msa0331130:CDS
ACAATTGTTGCTGGAACTTT+TGG	0.226271	2_4:-46514068	Msa0331130:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATAGAAAAAAGTAGCATGGT+GGG	+	chr2_4:46513856-46513875	None:intergenic	30.0%
CATTTGGTTATTTATCTAGC+TGG	+	chr2_4:46514003-46514022	None:intergenic	30.0%
TTATGGACAGACAAAATTGA+AGG	-	chr2_4:46514480-46514499	Msa0331130:intron	30.0%
TAAAGAGTTTGACATGAAGA+AGG	+	chr2_4:46514510-46514529	None:intergenic	30.0%
CATTCTATGATCTTTCTTCT+TGG	-	chr2_4:46514594-46514613	Msa0331130:intron	30.0%
TATGGAAACAGCTATAATCA+GGG	+	chr2_4:46514928-46514947	None:intergenic	30.0%
TTATGGAAACAGCTATAATC+AGG	+	chr2_4:46514929-46514948	None:intergenic	30.0%
TTTGAAAGAACAAAAGTAGG+AGG	+	chr2_4:46515081-46515100	None:intergenic	30.0%
TATCTGTGCTTCATTGTAAT+AGG	-	chr2_4:46515269-46515288	Msa0331130:intron	30.0%
CTGAACAACAAAGACATTAT+TGG	-	chr2_4:46515611-46515630	Msa0331130:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_4	gene	46513297	46517230	46513297	ID=Msa0331130;Name=Msa0331130
chr2_4	mRNA	46513297	46517230	46513297	ID=Msa0331130-mRNA-1;Parent=Msa0331130;Name=Msa0331130-mRNA-1;_AED=0.16;_eAED=0.16;_QI=0\|0\|0\|0.66\|1\|1\|6\|0\|428
chr2_4	exon	46517018	46517230	46517018	ID=Msa0331130-mRNA-1:exon:14115;Parent=Msa0331130-mRNA-1
chr2_4	exon	46515477	46515618	46515477	ID=Msa0331130-mRNA-1:exon:14114;Parent=Msa0331130-mRNA-1
chr2_4	exon	46514655	46514993	46514655	ID=Msa0331130-mRNA-1:exon:14113;Parent=Msa0331130-mRNA-1
chr2_4	exon	46514054	46514308	46514054	ID=Msa0331130-mRNA-1:exon:14112;Parent=Msa0331130-mRNA-1
chr2_4	exon	46513837	46513970	46513837	ID=Msa0331130-mRNA-1:exon:14111;Parent=Msa0331130-mRNA-1
chr2_4	exon	46513297	46513500	46513297	ID=Msa0331130-mRNA-1:exon:14110;Parent=Msa0331130-mRNA-1
chr2_4	CDS	46517018	46517230	46517018	ID=Msa0331130-mRNA-1:cds;Parent=Msa0331130-mRNA-1
chr2_4	CDS	46515477	46515618	46515477	ID=Msa0331130-mRNA-1:cds;Parent=Msa0331130-mRNA-1
chr2_4	CDS	46514655	46514993	46514655	ID=Msa0331130-mRNA-1:cds;Parent=Msa0331130-mRNA-1
chr2_4	CDS	46514054	46514308	46514054	ID=Msa0331130-mRNA-1:cds;Parent=Msa0331130-mRNA-1
chr2_4	CDS	46513837	46513970	46513837	ID=Msa0331130-mRNA-1:cds;Parent=Msa0331130-mRNA-1
chr2_4	CDS	46513297	46513500	46513297	ID=Msa0331130-mRNA-1:cds;Parent=Msa0331130-mRNA-1

Gene Sequence

>Msa0331130

ATGTATGAACCCATTTGGGGTGGTTGGCTTGGGATCTTGAAGTGTGCTCCTCTCAAGGTCTCAGGTTCGATTCCCTATAGTGCCAATTTCGGTGGACAAGTTCATACAGAGCTTTGCTCTTGCTTAGAACGGACCCCTGCAAGTGGCCAATTTCGGTGGACAAGTTCATACAGAGCTTTGCTCTTGCTTAGAACGGACCTCTGCAAGTGGACGCAGTATATGATGAAATCCCTGATTATAGCTGTTTCCATAACCATATGCTTCATTGCTTTTGTCATATCTAAGATTTTTATCTCAATCCTCCTCTACAAAAGATGGAGAAGAAAGCATATGATTTATGAAGAAGGTTATACAGGAGGGAAGATGGTGATATTTAGGTCTTCAATCCTAAAATCTTTAACAACTGATATGGTATTAAAGAAGACACAGAAACTGAACAACAAAGACATTATTGGTTCTGGTGGTTATGGCGTGGTTTATGAACTAAAACTCGACGATTCCGCGGCCTTTGCTGTGAAAAGGTTGAACCGCGGAACTGCTGAGAGAGATAAAGGTTTCGAGAGAGAGTTGCAAGCAATGGCAGACATAAAACACCGCAATGTTGTGACTCTTCATGGATATTACACTGCACCACACTACAATCTTCTCATATATGAGTTAATGCCTAATGGAAGTTTGGATTCCTTTTTACATGGAAGATCAATGAACAAGAAGGTTTTGGATTGGCCAACAAGACAAAGAATAGCTTTAGGTGCAGCAAGAGGAATATCATATCTTCACCATGATTGTATCCCTCACATTATCCACAGAGACATCAAGTCAAGTAATATATTGTTGGATCAAAATATGGAGGCTCGAGTTTCAGATTTCGGATTAGCCACATTGATGGAACCAAACAAAACTCATGTTTCAACAATTGTTGCTGGAACTTTTGGATACTTGGCTCCGGAATATTTTGATACAGGAAGAGCAACAATCAAAGGGGATGTTTACAGTTTTGGAGTTGTGTTACTGGAGCTTTTAACCGGAAAGAAACCTAGCGATGAATCATTCATGGAAGAGGGAACCAAACTTGTCACATGGGTGAAAGCTGTTGTTCAAGAGAGGAAGGAGGAGTTAGTTCTTGATAGTAGCTTAGGGTCATGTTGTCCGATGCATGAGGTCAACAAGGTGTTTAACATTGCAATGATGTGTCTTGAACCGGACCCTTTGAACAGACCAACCATGGCTGAGGTTGTCAACTTGCTCGAGAAAACACAGACGGATAGAGTTGTTACAGCATCCTGA

Protein sequence

>Msa0331130

MYEPIWGGWLGILKCAPLKVSGSIPYSANFGGQVHTELCSCLERTPASGQFRWTSSYRALLLLRTDLCKWTQYMMKSLIIAVSITICFIAFVISKIFISILLYKRWRRKHMIYEEGYTGGKMVIFRSSILKSLTTDMVLKKTQKLNNKDIIGSGGYGVVYELKLDDSAAFAVKRLNRGTAERDKGFERELQAMADIKHRNVVTLHGYYTAPHYNLLIYELMPNGSLDSFLHGRSMNKKVLDWPTRQRIALGAARGISYLHHDCIPHIIHRDIKSSNILLDQNMEARVSDFGLATLMEPNKTHVSTIVAGTFGYLAPEYFDTGRATIKGDVYSFGVVLLELLTGKKPSDESFMEEGTKLVTWVKAVVQERKEELVLDSSLGSCCPMHEVNKVFNIAMMCLEPDPLNRPTMAEVVNLLEKTQTDRVVTAS*