AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0331200

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0331200 MtrunA17_Chr2g0314001 90.141 142 12 1 117 258 870 1009 4.29e-81 262
Msa0331200 MtrunA17_Chr2g0314021 89.437 142 13 1 117 258 870 1009 4.49e-80 259
Msa0331200 MtrunA17_Chr5g0436871 73.759 141 35 1 118 258 73 211 2.17e-68 210
Msa0331200 MtrunA17_Chr2g0314031 81.690 142 15 2 117 258 866 996 2.70e-68 227
Msa0331200 MtrunA17_Chr7g0248261 68.590 156 46 2 104 258 807 960 6.82e-63 212
Msa0331200 MtrunA17_Chr5g0436911 61.628 172 33 2 118 258 122 291 8.47e-61 193
Msa0331200 MtrunA17_Chr5g0436851 79.832 119 22 1 139 257 39 155 2.11e-60 187
Msa0331200 MtrunA17_Chr1g0159871 72.340 141 37 1 118 258 798 936 1.76e-58 199
Msa0331200 MtrunA17_Chr1g0159881 68.657 134 40 1 116 249 337 468 1.28e-57 190
Msa0331200 MtrunA17_Chr5g0436861 65.957 141 46 1 118 258 871 1009 2.57e-53 185
Msa0331200 MtrunA17_Chr5g0436841 66.434 143 41 2 116 258 915 1050 1.84e-52 182
Msa0331200 MtrunA17_Chr5g0437331 66.912 136 39 2 118 253 872 1001 1.46e-49 174
Msa0331200 MtrunA17_Chr5g0436941 63.704 135 46 2 119 253 866 997 4.88e-46 164
Msa0331200 MtrunA17_Chr5g0436931 63.889 108 37 1 146 253 2 107 6.20e-43 142
Msa0331200 MtrunA17_Chr3g0078621 70.213 94 28 0 165 258 2 95 1.12e-40 135
Msa0331200 MtrunA17_Chr8g0366461 44.776 134 69 2 120 253 876 1004 5.10e-30 118
Msa0331200 MtrunA17_Chr5g0408401 42.857 140 74 2 123 258 648 785 1.49e-28 114
Msa0331200 MtrunA17_Chr8g0351191 47.706 109 57 0 2 110 7 115 9.34e-28 111
Msa0331200 MtrunA17_Chr5g0408621 41.781 146 72 3 119 256 915 1055 2.22e-27 110
Msa0331200 MtrunA17_Chr3g0113221 43.796 137 73 2 123 257 874 1008 2.53e-27 110
Msa0331200 MtrunA17_Chr5g0408421 41.912 136 66 3 129 256 872 1002 1.28e-26 108
Msa0331200 MtrunA17_Chr5g0408581 44.755 143 72 3 119 256 881 1021 2.79e-26 107
Msa0331200 MtrunA17_Chr8g0373071 44.037 109 61 0 2 110 594 702 6.58e-26 106
Msa0331200 MtrunA17_Chr3g0101191 46.154 104 55 1 2 104 581 684 7.41e-26 106
Msa0331200 MtrunA17_Chr6g0464171 42.000 150 72 4 117 256 877 1021 8.71e-26 106
Msa0331200 MtrunA17_Chr5g0408511 42.553 141 72 3 119 256 877 1011 1.01e-25 105
Msa0331200 MtrunA17_Chr5g0408741 42.553 141 74 3 119 256 785 921 1.89e-25 105
Msa0331200 MtrunA17_Chr2g0300201 42.000 150 72 4 117 256 877 1021 2.98e-25 104
Msa0331200 MtrunA17_Chr6g0464141 43.333 150 71 5 117 256 877 1022 4.40e-25 103
Msa0331200 MtrunA17_Chr6g0464161 42.000 150 72 4 117 256 606 750 6.09e-25 103
Msa0331200 MtrunA17_Chr6g0464211 42.000 150 72 4 117 256 876 1020 6.82e-25 103
Msa0331200 MtrunA17_Chr5g0408811 44.526 137 68 4 125 256 856 989 1.23e-24 102
Msa0331200 MtrunA17_Chr1g0196011 42.446 139 72 2 120 252 708 844 5.47e-24 100
Msa0331200 MtrunA17_Chr5g0408571 42.361 144 68 4 123 256 361 499 6.12e-24 100
Msa0331200 MtrunA17_Chr7g0241921 39.041 146 72 3 126 256 859 1002 6.39e-24 100
Msa0331200 MtrunA17_Chr5g0408611 39.041 146 76 3 119 256 868 1008 6.85e-24 100
Msa0331200 MtrunA17_Chr5g0408801 39.860 143 79 2 119 256 881 1021 9.33e-24 100
Msa0331200 MtrunA17_Chr5g0403401 38.356 146 77 3 119 256 869 1009 1.57e-23 99.8
Msa0331200 MtrunA17_Chr1g0196031 42.857 140 70 3 120 252 916 1052 7.44e-23 97.4
Msa0331200 MtrunA17_Chr8g0367351 42.308 130 67 2 130 253 887 1014 1.13e-22 97.1
Msa0331200 MtrunA17_Chr5g0419221 42.222 135 67 4 129 256 890 1020 1.47e-22 96.7
Msa0331200 MtrunA17_Chr5g0408821 37.589 141 81 2 119 254 274 412 1.67e-22 95.5
Msa0331200 MtrunA17_Chr6g0472051 45.161 124 58 4 116 234 80 198 2.82e-22 91.3
Msa0331200 MtrunA17_Chr6g0464201 51.579 95 41 3 116 208 15 106 3.78e-22 88.6
Msa0331200 MtrunA17_Chr5g0407241 38.462 130 67 3 135 256 902 1026 5.00e-22 95.1
Msa0331200 MtrunA17_Chr5g0408431 43.885 139 69 4 125 256 883 1019 8.62e-22 94.4
Msa0331200 MtrunA17_Chr8g0390891 42.000 100 57 1 2 101 213 311 1.22e-20 91.3
Msa0331200 MtrunA17_Chr1g0196001 51.807 83 38 1 120 202 876 956 1.46e-19 87.8
Msa0331200 MtrunA17_Chr3g0144121 41.000 100 58 1 2 101 582 680 2.26e-19 87.4
Msa0331200 MtrunA17_Chr7g0219271 38.614 101 62 0 1 101 129 229 4.74e-19 85.9
Msa0331200 MtrunA17_Chr8g0369631 39.669 121 65 3 143 257 2 120 6.18e-19 80.1
Msa0331200 MtrunA17_Chr7g0229241 39.091 110 66 1 1 110 523 631 1.48e-18 85.1
Msa0331200 MtrunA17_Chr5g0408501 42.017 119 62 3 143 256 2 118 2.27e-18 78.6
Msa0331200 MtrunA17_Chr8g0361231 37.500 128 69 3 131 256 958 1076 7.55e-18 82.8
Msa0331200 MtrunA17_Chr8g0363501 38.281 128 66 4 131 255 402 519 1.19e-17 82.0
Msa0331200 MtrunA17_Chr8g0363061 39.370 127 66 3 131 255 862 979 2.98e-17 81.3
Msa0331200 MtrunA17_Chr8g0352041 50.000 74 35 1 140 213 37 108 3.03e-17 75.5
Msa0331200 MtrunA17_Chr8g0362961 37.795 127 68 3 131 255 1106 1223 3.52e-17 80.9
Msa0331200 MtrunA17_Chr8g0362981 36.000 125 73 2 131 255 622 739 5.11e-17 80.5
Msa0331200 MtrunA17_Chr1g0176301 37.302 126 68 3 131 254 621 737 6.77e-17 80.1
Msa0331200 MtrunA17_Chr8g0362391 36.800 125 72 2 131 255 208 325 6.86e-17 79.0
Msa0331200 MtrunA17_Chr8g0363601 34.783 138 76 4 121 255 647 773 1.00e-16 79.7
Msa0331200 MtrunA17_Chr8g0362871 34.815 135 80 3 121 255 647 773 1.30e-16 79.3
Msa0331200 MtrunA17_Chr3g0127701 30.323 155 85 2 120 253 841 993 1.55e-16 79.0
Msa0331200 MtrunA17_Chr8g0362911 36.508 126 69 3 132 255 606 722 2.24e-16 78.6
Msa0331200 MtrunA17_Chr8g0363261 34.815 135 80 3 121 255 257 383 2.46e-16 77.8
Msa0331200 MtrunA17_Chr2g0284121 33.333 126 77 2 131 256 1095 1213 3.38e-16 78.2
Msa0331200 MtrunA17_Chr8g0363661 35.507 138 75 4 121 255 710 836 3.47e-16 77.8
Msa0331200 MtrunA17_Chr8g0363691 35.938 128 69 3 131 255 1130 1247 4.02e-16 77.8
Msa0331200 MtrunA17_Chr8g0362941 34.074 135 81 5 121 255 613 739 4.04e-16 77.8
Msa0331200 MtrunA17_Chr8g0363401 35.200 125 74 2 131 255 671 788 5.11e-16 77.4
Msa0331200 MtrunA17_Chr8g0362841 37.008 127 69 3 131 255 708 825 6.86e-16 77.0
Msa0331200 MtrunA17_Chr8g0363281 34.375 128 71 3 131 255 639 756 7.70e-16 77.0
Msa0331200 MtrunA17_Chr8g0363191 36.719 128 70 3 131 256 583 701 8.34e-16 76.6
Msa0331200 MtrunA17_Chr3g0095201 34.127 126 76 2 131 256 870 988 9.29e-16 76.6
Msa0331200 MtrunA17_Chr4g0013491 36.220 127 69 4 131 255 619 735 9.75e-16 76.6
Msa0331200 MtrunA17_Chr8g0362431 35.200 125 74 2 131 255 664 781 9.92e-16 76.6
Msa0331200 MtrunA17_Chr8g0363111 36.220 127 68 4 132 255 867 983 1.07e-15 76.6
Msa0331200 MtrunA17_Chr3g0127671 29.032 155 87 2 120 253 886 1038 1.47e-15 76.3
Msa0331200 MtrunA17_Chr8g0375511 34.815 135 80 4 121 252 997 1126 1.86e-15 75.9
Msa0331200 MtrunA17_Chr8g0380951 30.827 133 87 2 120 249 836 966 2.06e-15 75.9
Msa0331200 MtrunA17_Chr8g0363241 34.400 125 75 2 131 255 624 741 2.19e-15 75.5
Msa0331200 MtrunA17_Chr8g0360851 36.220 127 70 4 131 255 890 1007 2.99e-15 75.1
Msa0331200 MtrunA17_Chr8g0363121 34.400 125 75 2 131 255 666 783 3.36e-15 75.1
Msa0331200 MtrunA17_Chr5g0409241 55.882 68 26 2 125 190 561 626 7.10e-15 73.9
Msa0331200 MtrunA17_Chr8g0362931 33.600 125 76 3 131 255 1051 1168 9.81e-15 73.9
Msa0331200 MtrunA17_Chr0c09g0493531 36.508 126 66 4 131 256 734 845 1.15e-14 73.6
Msa0331200 MtrunA17_Chr1g0153361 43.210 81 46 0 4 84 914 994 2.12e-14 72.8
Msa0331200 MtrunA17_Chr3g0107231 43.038 79 44 1 27 104 112 190 1.25e-13 69.7
Msa0331200 MtrunA17_Chr2g0284191 35.484 124 66 3 131 254 725 834 1.26e-13 70.5
Msa0331200 MtrunA17_Chr4g0069101 33.673 98 65 0 1 98 263 360 1.55e-13 70.1
Msa0331200 MtrunA17_Chr2g0279301 44.444 72 40 0 29 100 207 278 2.16e-13 68.9
Msa0331200 MtrunA17_Chr8g0363641 34.921 126 66 4 132 255 139 250 2.46e-13 67.8
Msa0331200 MtrunA17_Chr4g0028041 37.255 102 58 3 121 221 18 114 4.00e-13 64.7
Msa0331200 MtrunA17_Chr8g0380941 31.469 143 86 6 120 254 867 1005 4.72e-13 68.6
Msa0331200 MtrunA17_Chr3g0122931 35.185 108 67 1 3 110 215 319 7.25e-13 68.2
Msa0331200 MtrunA17_Chr3g0129391 32.090 134 80 2 121 254 1030 1152 1.54e-12 67.4
Msa0331200 MtrunA17_Chr8g0340051 34.286 140 76 6 121 257 184 310 3.16e-12 65.5
Msa0331200 MtrunA17_Chr1g0179941 33.645 107 69 1 4 110 127 231 5.71e-12 65.1
Msa0331200 MtrunA17_Chr1g0201011 42.308 78 42 2 127 203 35 110 6.46e-12 61.2
Msa0331200 MtrunA17_Chr8g0363371 34.167 120 66 3 139 255 718 827 7.40e-12 65.1
Msa0331200 MtrunA17_Chr4g0056611 33.645 107 69 1 4 110 127 231 8.82e-12 65.1
Msa0331200 MtrunA17_Chr3g0117621 33.645 107 69 1 5 111 663 767 1.22e-11 64.7
Msa0331200 MtrunA17_Chr7g0271061 33.645 107 69 1 5 111 663 767 1.22e-11 64.7
Msa0331200 MtrunA17_Chr2g0279231 27.007 137 93 2 4 135 104 238 1.57e-11 64.3
Msa0331200 MtrunA17_Chr1g0196051 49.206 63 30 1 139 201 5 65 1.90e-11 58.5
Msa0331200 MtrunA17_Chr7g0236651 31.206 141 78 5 121 257 640 765 3.98e-11 62.8
Msa0331200 MtrunA17_Chr8g0340091 33.103 145 77 7 118 257 684 813 4.92e-11 62.8
Msa0331200 MtrunA17_Chr8g0340081 32.168 143 81 6 118 257 659 788 4.93e-11 62.8
Msa0331200 MtrunA17_Chr3g0145631 38.372 86 50 2 121 205 116 199 7.08e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0331200 AT3G47580.1 40.441 136 72 3 122 256 875 1002 5.06e-27 110
Msa0331200 AT3G47570.1 40.714 140 66 4 122 256 874 1001 5.93e-24 101
Msa0331200 AT3G47090.1 36.765 136 77 3 122 256 873 1000 5.88e-22 95.5
Msa0331200 AT3G47110.1 40.000 135 73 3 123 256 892 1019 2.59e-21 93.6
Msa0331200 AT5G20480.1 36.296 135 68 3 122 256 884 1000 3.36e-18 84.3
Msa0331200 AT5G20480.2 36.296 135 68 3 122 256 884 1000 3.36e-18 84.3
Msa0331200 AT5G39390.1 36.691 139 62 4 122 256 381 497 1.25e-17 82.4
Msa0331200 AT2G24130.1 31.081 148 95 3 116 258 818 963 3.41e-16 78.6
Msa0331200 AT2G24130.2 31.081 148 95 3 116 258 833 978 3.52e-16 78.6
Msa0331200 AT4G39400.1 34.328 134 77 3 121 254 1038 1160 6.90e-13 68.6
Msa0331200 AT2G01950.1 31.884 138 87 3 121 253 995 1130 8.80e-13 68.6
Msa0331200 AT5G07280.1 33.588 131 69 4 126 252 1076 1192 9.47e-12 65.5
Msa0331200 ATMG00710.1 43.548 62 35 0 49 110 1 62 2.48e-11 60.1

Find 59 sgRNAs with CRISPR-Local

Find 176 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGTTTACCTAAGAGTTTC+TGG 0.108164 2_4:+46682731 Msa0331200:CDS
AGTTAATTCATCCACAATTA+AGG 0.193569 2_4:+46683273 Msa0331200:CDS
TTAGTAATGTTTGCAATAAT+TGG 0.199833 2_4:+46683706 Msa0331200:CDS
CCATATTGTTGCGGTGTTCT+AGG 0.260485 2_4:-46682649 None:intergenic
GTTTCACCCCAGAAACTCTT+AGG 0.288367 2_4:-46682739 None:intergenic
CACAATCCCTGTCTCATCTT+CGG 0.309579 2_4:-46683662 None:intergenic
TTGAAGTCTATTCCCGTTGA+TGG 0.318460 2_4:-46682796 None:intergenic
ACTCATGAATAGGACCCTTT+TGG 0.328758 2_4:+46682681 Msa0331200:CDS
TTCCCGCTCCATACTTTCAT+AGG 0.334008 2_4:-46682823 None:intergenic
CCAGAAAACATGCTATTTGT+TGG 0.338639 2_4:-46683553 None:intergenic
TGGACATCTGTTGATCAAAT+AGG 0.341183 2_4:-46682776 None:intergenic
CCTGTCTCATCTTCGGGAAA+TGG 0.360866 2_4:-46683655 None:intergenic
CGAGTGATGTGTATGCTTCT+AGG 0.363445 2_4:+46682706 Msa0331200:CDS
GGATTAGCTAGGCTAATTCA+TGG 0.381868 2_4:+46683224 Msa0331200:intron
TCAAGACACCTATGAAAGTA+TGG 0.385730 2_4:+46682815 Msa0331200:CDS
CCTAGAACACCGCAACAATA+TGG 0.389185 2_4:+46682649 Msa0331200:CDS
TGGTTTACCTAAGAGTTTCT+GGG 0.389991 2_4:+46682732 Msa0331200:CDS
GTTAATTCATCCACAATTAA+GGG 0.410908 2_4:+46683274 Msa0331200:CDS
CAAATCAATGTTTCCATGCT+AGG 0.422907 2_4:+46683801 Msa0331200:CDS
GAAAGTATGGAGCGGGAAAC+CGG 0.429960 2_4:+46682828 Msa0331200:CDS
ACAATCCCTGTCTCATCTTC+GGG 0.433460 2_4:-46683661 None:intergenic
CCATTTCCCGAAGATGAGAC+AGG 0.442291 2_4:+46683655 Msa0331200:CDS
ATTGCTCTGAAACAAACTTC+AGG 0.449624 2_4:-46682579 None:intergenic
ATGGCGATATCTACAGTTAC+GGG 0.456646 2_4:+46683491 Msa0331200:CDS
ATCAACAGATGTCCATCAAC+GGG 0.466023 2_4:+46682784 Msa0331200:CDS
TCCACAATTAAGGGAACTAT+AGG 0.466540 2_4:+46683283 Msa0331200:CDS
TACACATCACTCGCTCCAAA+AGG 0.467612 2_4:-46682696 None:intergenic
GGCAGACTACTCATCTTTGA+AGG 0.479159 2_4:+46682849 Msa0331200:CDS
CATGGCGATATCTACAGTTA+CGG 0.480812 2_4:+46683490 Msa0331200:CDS
GTCTTGTTGAACTCATGAAT+AGG 0.493551 2_4:+46682671 Msa0331200:CDS
TTAGAAATGCTCACAGTCAC+AGG 0.501614 2_4:+46683523 Msa0331200:CDS
TCCTATAGTTCCCTTAATTG+TGG 0.506052 2_4:-46683284 None:intergenic
ACTATCTCAAAGATTCCTGC+TGG 0.507916 2_4:-46683613 None:intergenic
CGAAGATGAGACAGGGATTG+TGG 0.508733 2_4:+46683663 Msa0331200:CDS
GGATTGTGGAAAACAAGATT+AGG 0.509316 2_4:+46683677 Msa0331200:CDS
ATGTGTATGCTTCTAGGAGT+TGG 0.512739 2_4:+46682712 Msa0331200:CDS
CACCTATGAAAGTATGGAGC+GGG 0.531243 2_4:+46682821 Msa0331200:CDS
TTCAAAGATGAGTAGTCTGC+CGG 0.533251 2_4:-46682847 None:intergenic
TCGATGAGCAAGAAACTCTT+CGG 0.536064 2_4:-46683740 None:intergenic
CAATGTTTCCATGCTAGGAT+CGG 0.550359 2_4:+46683806 Msa0331200:exon
TTGTTGCGGTGTTCTAGGTA+CGG 0.552428 2_4:-46682644 None:intergenic
CATTTCCCGAAGATGAGACA+GGG 0.554827 2_4:+46683656 Msa0331200:CDS
ACTATAGGATATGTTCCTCC+TGG 0.564251 2_4:+46683298 Msa0331200:CDS
ACACATCACTCGCTCCAAAA+GGG 0.573259 2_4:-46682695 None:intergenic
TACAAGATGAAAGTTCCAGC+AGG 0.573561 2_4:+46683598 Msa0331200:CDS
GCAAGTTGAAAGGAATCAAG+AGG 0.574882 2_4:+46682614 Msa0331200:CDS
GGTTTACCTAAGAGTTTCTG+GGG 0.599316 2_4:+46682733 Msa0331200:CDS
CAAATGGGAACTAAACTGAA+AGG 0.601089 2_4:+46682538 None:intergenic
GCAAGCAACCGATCCTAGCA+TGG 0.603355 2_4:-46683814 None:intergenic
GTCTGAGAAGAGTATGGAGA+GGG 0.603432 2_4:+46683451 Msa0331200:intron
GATCAACAGATGTCCATCAA+CGG 0.605193 2_4:+46682783 Msa0331200:CDS
TCAACAAGACCATATTGTTG+CGG 0.627370 2_4:-46682658 None:intergenic
GGTGGCAAAGTATTACCTCA+TGG 0.629995 2_4:+46683472 Msa0331200:CDS
TGAGAAGAGTATGGAGAGGG+TGG 0.636478 2_4:+46683454 Msa0331200:intron
AAAGGTTTGAGAACTGACAA+TGG 0.670169 2_4:+46682556 Msa0331200:CDS
ACACCTATGAAAGTATGGAG+CGG 0.697340 2_4:+46682820 Msa0331200:CDS
GGGTGAAACAATGACAACTG+TGG 0.726121 2_4:+46682753 Msa0331200:CDS
TGTCTGAGAAGAGTATGGAG+AGG 0.746987 2_4:+46683450 Msa0331200:intron
TAACTGTAGATATCGCCATG+AGG 0.751272 2_4:-46683487 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAAAGTAAATTATAATTACA+AGG - chr2_4:46683336-46683355 None:intergenic 15.0%
!! GAAAGTAAATTATAATTACA+AGG - chr2_4:46683336-46683355 None:intergenic 15.0%
!! TTAGTAATGTTTGCAATAAT+TGG + chr2_4:46683706-46683725 Msa0331200:CDS 20.0%
!!! TTTGCTATAACATCTTTTAT+CGG - chr2_4:46683769-46683788 None:intergenic 20.0%
!! TTAGTAATGTTTGCAATAAT+TGG + chr2_4:46683706-46683725 Msa0331200:CDS 20.0%
!!! TTTGCTATAACATCTTTTAT+CGG - chr2_4:46683769-46683788 None:intergenic 20.0%
!! AAGAAATTTGAACCAGTTTT+AGG + chr2_4:46682950-46682969 Msa0331200:intron 25.0%
! CATTCCAATTGTTGTAAATT+CGG - chr2_4:46683073-46683092 None:intergenic 25.0%
! TGACAGATAAGTAAACAAAT+TGG + chr2_4:46683132-46683151 Msa0331200:intron 25.0%
! AGTTAATTCATCCACAATTA+AGG + chr2_4:46683273-46683292 Msa0331200:CDS 25.0%
! GTTAATTCATCCACAATTAA+GGG + chr2_4:46683274-46683293 Msa0331200:CDS 25.0%
!! AAGAAATTTGAACCAGTTTT+AGG + chr2_4:46682950-46682969 Msa0331200:intron 25.0%
! CATTCCAATTGTTGTAAATT+CGG - chr2_4:46683073-46683092 None:intergenic 25.0%
! TGACAGATAAGTAAACAAAT+TGG + chr2_4:46683132-46683151 Msa0331200:intron 25.0%
! AGTTAATTCATCCACAATTA+AGG + chr2_4:46683273-46683292 Msa0331200:CDS 25.0%
! GTTAATTCATCCACAATTAA+GGG + chr2_4:46683274-46683293 Msa0331200:CDS 25.0%
!! AATGAGTTTTGCAAGTTGAA+AGG + chr2_4:46682604-46682623 Msa0331200:CDS 30.0%
TTCAAATTTCTTGTCTTGCT+TGG - chr2_4:46682942-46682961 None:intergenic 30.0%
CATAGAAAAACAAAGATGTG+TGG - chr2_4:46683038-46683057 None:intergenic 30.0%
!!! CACACATCTTTGTTTTTCTA+TGG + chr2_4:46683036-46683055 Msa0331200:intron 30.0%
TTGTCCGAATTTACAACAAT+TGG + chr2_4:46683066-46683085 Msa0331200:intron 30.0%
TTATCTGTCAACCATAAAAG+AGG - chr2_4:46683122-46683141 None:intergenic 30.0%
!! TGTTCTTCTTGATGATGATA+TGG + chr2_4:46683181-46683200 Msa0331200:intron 30.0%
!! AATGAGTTTTGCAAGTTGAA+AGG + chr2_4:46682604-46682623 Msa0331200:CDS 30.0%
TTCAAATTTCTTGTCTTGCT+TGG - chr2_4:46682942-46682961 None:intergenic 30.0%
CATAGAAAAACAAAGATGTG+TGG - chr2_4:46683038-46683057 None:intergenic 30.0%
!!! CACACATCTTTGTTTTTCTA+TGG + chr2_4:46683036-46683055 Msa0331200:intron 30.0%
TTGTCCGAATTTACAACAAT+TGG + chr2_4:46683066-46683085 Msa0331200:intron 30.0%
TTATCTGTCAACCATAAAAG+AGG - chr2_4:46683122-46683141 None:intergenic 30.0%
!! TGTTCTTCTTGATGATGATA+TGG + chr2_4:46683181-46683200 Msa0331200:intron 30.0%
AAAGGTTTGAGAACTGACAA+TGG + chr2_4:46682556-46682575 Msa0331200:CDS 35.0%
!! ATTGCTCTGAAACAAACTTC+AGG - chr2_4:46682582-46682601 None:intergenic 35.0%
TCAACAAGACCATATTGTTG+CGG - chr2_4:46682661-46682680 None:intergenic 35.0%
! GTCTTGTTGAACTCATGAAT+AGG + chr2_4:46682671-46682690 Msa0331200:CDS 35.0%
TTGGTTTACCTAAGAGTTTC+TGG + chr2_4:46682731-46682750 Msa0331200:CDS 35.0%
TGGTTTACCTAAGAGTTTCT+GGG + chr2_4:46682732-46682751 Msa0331200:CDS 35.0%
TGGACATCTGTTGATCAAAT+AGG - chr2_4:46682779-46682798 None:intergenic 35.0%
TCAAGACACCTATGAAAGTA+TGG + chr2_4:46682815-46682834 Msa0331200:CDS 35.0%
AACAACAACAGTCCTAAAAC+TGG - chr2_4:46682965-46682984 None:intergenic 35.0%
! CAACATGATCTCCTCTTTTA+TGG + chr2_4:46683108-46683127 Msa0331200:intron 35.0%
TCCACAATTAAGGGAACTAT+AGG + chr2_4:46683283-46683302 Msa0331200:CDS 35.0%
TCCTATAGTTCCCTTAATTG+TGG - chr2_4:46683287-46683306 None:intergenic 35.0%
GACAACAACATCAACAGTTT+GGG - chr2_4:46683434-46683453 None:intergenic 35.0%
AGACAACAACATCAACAGTT+TGG - chr2_4:46683435-46683454 None:intergenic 35.0%
! CCAGAAAACATGCTATTTGT+TGG - chr2_4:46683556-46683575 None:intergenic 35.0%
! CCAACAAATAGCATGTTTTC+TGG + chr2_4:46683553-46683572 Msa0331200:CDS 35.0%
GGATTGTGGAAAACAAGATT+AGG + chr2_4:46683677-46683696 Msa0331200:CDS 35.0%
CAAATCAATGTTTCCATGCT+AGG + chr2_4:46683801-46683820 Msa0331200:CDS 35.0%
AAAGGTTTGAGAACTGACAA+TGG + chr2_4:46682556-46682575 Msa0331200:CDS 35.0%
!! ATTGCTCTGAAACAAACTTC+AGG - chr2_4:46682582-46682601 None:intergenic 35.0%
TCAACAAGACCATATTGTTG+CGG - chr2_4:46682661-46682680 None:intergenic 35.0%
! GTCTTGTTGAACTCATGAAT+AGG + chr2_4:46682671-46682690 Msa0331200:CDS 35.0%
TTGGTTTACCTAAGAGTTTC+TGG + chr2_4:46682731-46682750 Msa0331200:CDS 35.0%
TGGTTTACCTAAGAGTTTCT+GGG + chr2_4:46682732-46682751 Msa0331200:CDS 35.0%
TGGACATCTGTTGATCAAAT+AGG - chr2_4:46682779-46682798 None:intergenic 35.0%
TCAAGACACCTATGAAAGTA+TGG + chr2_4:46682815-46682834 Msa0331200:CDS 35.0%
AACAACAACAGTCCTAAAAC+TGG - chr2_4:46682965-46682984 None:intergenic 35.0%
! CAACATGATCTCCTCTTTTA+TGG + chr2_4:46683108-46683127 Msa0331200:intron 35.0%
TCCACAATTAAGGGAACTAT+AGG + chr2_4:46683283-46683302 Msa0331200:CDS 35.0%
TCCTATAGTTCCCTTAATTG+TGG - chr2_4:46683287-46683306 None:intergenic 35.0%
GACAACAACATCAACAGTTT+GGG - chr2_4:46683434-46683453 None:intergenic 35.0%
AGACAACAACATCAACAGTT+TGG - chr2_4:46683435-46683454 None:intergenic 35.0%
! CCAGAAAACATGCTATTTGT+TGG - chr2_4:46683556-46683575 None:intergenic 35.0%
! CCAACAAATAGCATGTTTTC+TGG + chr2_4:46683553-46683572 Msa0331200:CDS 35.0%
GGATTGTGGAAAACAAGATT+AGG + chr2_4:46683677-46683696 Msa0331200:CDS 35.0%
CAAATCAATGTTTCCATGCT+AGG + chr2_4:46683801-46683820 Msa0331200:CDS 35.0%
! GCAAGTTGAAAGGAATCAAG+AGG + chr2_4:46682614-46682633 Msa0331200:CDS 40.0%
! ACTCATGAATAGGACCCTTT+TGG + chr2_4:46682681-46682700 Msa0331200:CDS 40.0%
! ATGTGTATGCTTCTAGGAGT+TGG + chr2_4:46682712-46682731 Msa0331200:CDS 40.0%
GGTTTACCTAAGAGTTTCTG+GGG + chr2_4:46682733-46682752 Msa0331200:CDS 40.0%
GATCAACAGATGTCCATCAA+CGG + chr2_4:46682783-46682802 Msa0331200:CDS 40.0%
ATCAACAGATGTCCATCAAC+GGG + chr2_4:46682784-46682803 Msa0331200:CDS 40.0%
TTGAAGTCTATTCCCGTTGA+TGG - chr2_4:46682799-46682818 None:intergenic 40.0%
! ACACCTATGAAAGTATGGAG+CGG + chr2_4:46682820-46682839 Msa0331200:CDS 40.0%
TTCAAAGATGAGTAGTCTGC+CGG - chr2_4:46682850-46682869 None:intergenic 40.0%
! GCAAAGGTAGTGGTAATCAA+TGG - chr2_4:46683014-46683033 None:intergenic 40.0%
AACAAAGATGTGTGGAGCAA+AGG - chr2_4:46683030-46683049 None:intergenic 40.0%
TAATCCAAAGTCTCCCAAGT+GGG - chr2_4:46683210-46683229 None:intergenic 40.0%
!! GGATTAGCTAGGCTAATTCA+TGG + chr2_4:46683224-46683243 Msa0331200:intron 40.0%
ACTATAGGATATGTTCCTCC+TGG + chr2_4:46683298-46683317 Msa0331200:CDS 40.0%
ACAAGGTGAAAGAACTTACC+AGG - chr2_4:46683319-46683338 None:intergenic 40.0%
GTTGTTGTCTGAGAAGAGTA+TGG + chr2_4:46683445-46683464 Msa0331200:intron 40.0%
TAACTGTAGATATCGCCATG+AGG - chr2_4:46683490-46683509 None:intergenic 40.0%
CATGGCGATATCTACAGTTA+CGG + chr2_4:46683490-46683509 Msa0331200:CDS 40.0%
ATGGCGATATCTACAGTTAC+GGG + chr2_4:46683491-46683510 Msa0331200:CDS 40.0%
TTAGAAATGCTCACAGTCAC+AGG + chr2_4:46683523-46683542 Msa0331200:CDS 40.0%
TACAAGATGAAAGTTCCAGC+AGG + chr2_4:46683598-46683617 Msa0331200:CDS 40.0%
ACTATCTCAAAGATTCCTGC+TGG - chr2_4:46683616-46683635 None:intergenic 40.0%
TCGATGAGCAAGAAACTCTT+CGG - chr2_4:46683743-46683762 None:intergenic 40.0%
CAATGTTTCCATGCTAGGAT+CGG + chr2_4:46683806-46683825 Msa0331200:exon 40.0%
! GCAAGTTGAAAGGAATCAAG+AGG + chr2_4:46682614-46682633 Msa0331200:CDS 40.0%
! ACTCATGAATAGGACCCTTT+TGG + chr2_4:46682681-46682700 Msa0331200:CDS 40.0%
! ATGTGTATGCTTCTAGGAGT+TGG + chr2_4:46682712-46682731 Msa0331200:CDS 40.0%
GGTTTACCTAAGAGTTTCTG+GGG + chr2_4:46682733-46682752 Msa0331200:CDS 40.0%
GATCAACAGATGTCCATCAA+CGG + chr2_4:46682783-46682802 Msa0331200:CDS 40.0%
ATCAACAGATGTCCATCAAC+GGG + chr2_4:46682784-46682803 Msa0331200:CDS 40.0%
TTGAAGTCTATTCCCGTTGA+TGG - chr2_4:46682799-46682818 None:intergenic 40.0%
! ACACCTATGAAAGTATGGAG+CGG + chr2_4:46682820-46682839 Msa0331200:CDS 40.0%
TTCAAAGATGAGTAGTCTGC+CGG - chr2_4:46682850-46682869 None:intergenic 40.0%
! GCAAAGGTAGTGGTAATCAA+TGG - chr2_4:46683014-46683033 None:intergenic 40.0%
AACAAAGATGTGTGGAGCAA+AGG - chr2_4:46683030-46683049 None:intergenic 40.0%
TAATCCAAAGTCTCCCAAGT+GGG - chr2_4:46683210-46683229 None:intergenic 40.0%
!! GGATTAGCTAGGCTAATTCA+TGG + chr2_4:46683224-46683243 Msa0331200:intron 40.0%
ACTATAGGATATGTTCCTCC+TGG + chr2_4:46683298-46683317 Msa0331200:CDS 40.0%
ACAAGGTGAAAGAACTTACC+AGG - chr2_4:46683319-46683338 None:intergenic 40.0%
GTTGTTGTCTGAGAAGAGTA+TGG + chr2_4:46683445-46683464 Msa0331200:intron 40.0%
TAACTGTAGATATCGCCATG+AGG - chr2_4:46683490-46683509 None:intergenic 40.0%
CATGGCGATATCTACAGTTA+CGG + chr2_4:46683490-46683509 Msa0331200:CDS 40.0%
ATGGCGATATCTACAGTTAC+GGG + chr2_4:46683491-46683510 Msa0331200:CDS 40.0%
TTAGAAATGCTCACAGTCAC+AGG + chr2_4:46683523-46683542 Msa0331200:CDS 40.0%
TACAAGATGAAAGTTCCAGC+AGG + chr2_4:46683598-46683617 Msa0331200:CDS 40.0%
ACTATCTCAAAGATTCCTGC+TGG - chr2_4:46683616-46683635 None:intergenic 40.0%
TCGATGAGCAAGAAACTCTT+CGG - chr2_4:46683743-46683762 None:intergenic 40.0%
CAATGTTTCCATGCTAGGAT+CGG + chr2_4:46683806-46683825 Msa0331200:exon 40.0%
! TTGTTGCGGTGTTCTAGGTA+CGG - chr2_4:46682647-46682666 None:intergenic 45.0%
CCATATTGTTGCGGTGTTCT+AGG - chr2_4:46682652-46682671 None:intergenic 45.0%
CCTAGAACACCGCAACAATA+TGG + chr2_4:46682649-46682668 Msa0331200:CDS 45.0%
ACACATCACTCGCTCCAAAA+GGG - chr2_4:46682698-46682717 None:intergenic 45.0%
TACACATCACTCGCTCCAAA+AGG - chr2_4:46682699-46682718 None:intergenic 45.0%
CGAGTGATGTGTATGCTTCT+AGG + chr2_4:46682706-46682725 Msa0331200:CDS 45.0%
GTTTCACCCCAGAAACTCTT+AGG - chr2_4:46682742-46682761 None:intergenic 45.0%
GGGTGAAACAATGACAACTG+TGG + chr2_4:46682753-46682772 Msa0331200:CDS 45.0%
! CACCTATGAAAGTATGGAGC+GGG + chr2_4:46682821-46682840 Msa0331200:CDS 45.0%
TTCCCGCTCCATACTTTCAT+AGG - chr2_4:46682826-46682845 None:intergenic 45.0%
GGCAGACTACTCATCTTTGA+AGG + chr2_4:46682849-46682868 Msa0331200:CDS 45.0%
TTGGCACATTTCACAGCTCT+AGG - chr2_4:46682923-46682942 None:intergenic 45.0%
TGATGATATGGTAGCCCACT+TGG + chr2_4:46683193-46683212 Msa0331200:intron 45.0%
GATGATATGGTAGCCCACTT+GGG + chr2_4:46683194-46683213 Msa0331200:intron 45.0%
CTAATCCAAAGTCTCCCAAG+TGG - chr2_4:46683211-46683230 None:intergenic 45.0%
!! TGGGAGACTTTGGATTAGCT+AGG + chr2_4:46683213-46683232 Msa0331200:intron 45.0%
AGGTGAAAGAACTTACCAGG+AGG - chr2_4:46683316-46683335 None:intergenic 45.0%
! TGTCTGAGAAGAGTATGGAG+AGG + chr2_4:46683450-46683469 Msa0331200:intron 45.0%
! GTCTGAGAAGAGTATGGAGA+GGG + chr2_4:46683451-46683470 Msa0331200:intron 45.0%
! GGTGGCAAAGTATTACCTCA+TGG + chr2_4:46683472-46683491 Msa0331200:CDS 45.0%
CATTTCCCGAAGATGAGACA+GGG + chr2_4:46683656-46683675 Msa0331200:CDS 45.0%
ACAATCCCTGTCTCATCTTC+GGG - chr2_4:46683664-46683683 None:intergenic 45.0%
CACAATCCCTGTCTCATCTT+CGG - chr2_4:46683665-46683684 None:intergenic 45.0%
! TTGTTGCGGTGTTCTAGGTA+CGG - chr2_4:46682647-46682666 None:intergenic 45.0%
CCATATTGTTGCGGTGTTCT+AGG - chr2_4:46682652-46682671 None:intergenic 45.0%
CCTAGAACACCGCAACAATA+TGG + chr2_4:46682649-46682668 Msa0331200:CDS 45.0%
ACACATCACTCGCTCCAAAA+GGG - chr2_4:46682698-46682717 None:intergenic 45.0%
TACACATCACTCGCTCCAAA+AGG - chr2_4:46682699-46682718 None:intergenic 45.0%
CGAGTGATGTGTATGCTTCT+AGG + chr2_4:46682706-46682725 Msa0331200:CDS 45.0%
GTTTCACCCCAGAAACTCTT+AGG - chr2_4:46682742-46682761 None:intergenic 45.0%
GGGTGAAACAATGACAACTG+TGG + chr2_4:46682753-46682772 Msa0331200:CDS 45.0%
! CACCTATGAAAGTATGGAGC+GGG + chr2_4:46682821-46682840 Msa0331200:CDS 45.0%
TTCCCGCTCCATACTTTCAT+AGG - chr2_4:46682826-46682845 None:intergenic 45.0%
GGCAGACTACTCATCTTTGA+AGG + chr2_4:46682849-46682868 Msa0331200:CDS 45.0%
TTGGCACATTTCACAGCTCT+AGG - chr2_4:46682923-46682942 None:intergenic 45.0%
TGATGATATGGTAGCCCACT+TGG + chr2_4:46683193-46683212 Msa0331200:intron 45.0%
GATGATATGGTAGCCCACTT+GGG + chr2_4:46683194-46683213 Msa0331200:intron 45.0%
CTAATCCAAAGTCTCCCAAG+TGG - chr2_4:46683211-46683230 None:intergenic 45.0%
!! TGGGAGACTTTGGATTAGCT+AGG + chr2_4:46683213-46683232 Msa0331200:intron 45.0%
AGGTGAAAGAACTTACCAGG+AGG - chr2_4:46683316-46683335 None:intergenic 45.0%
! TGTCTGAGAAGAGTATGGAG+AGG + chr2_4:46683450-46683469 Msa0331200:intron 45.0%
! GTCTGAGAAGAGTATGGAGA+GGG + chr2_4:46683451-46683470 Msa0331200:intron 45.0%
! GGTGGCAAAGTATTACCTCA+TGG + chr2_4:46683472-46683491 Msa0331200:CDS 45.0%
CATTTCCCGAAGATGAGACA+GGG + chr2_4:46683656-46683675 Msa0331200:CDS 45.0%
ACAATCCCTGTCTCATCTTC+GGG - chr2_4:46683664-46683683 None:intergenic 45.0%
CACAATCCCTGTCTCATCTT+CGG - chr2_4:46683665-46683684 None:intergenic 45.0%
! GAAAGTATGGAGCGGGAAAC+CGG + chr2_4:46682828-46682847 Msa0331200:CDS 50.0%
GATGTGTGGAGCAAAGGTAG+TGG - chr2_4:46683024-46683043 None:intergenic 50.0%
! TGAGAAGAGTATGGAGAGGG+TGG + chr2_4:46683454-46683473 Msa0331200:intron 50.0%
CCTGTCTCATCTTCGGGAAA+TGG - chr2_4:46683658-46683677 None:intergenic 50.0%
CCATTTCCCGAAGATGAGAC+AGG + chr2_4:46683655-46683674 Msa0331200:CDS 50.0%
CGAAGATGAGACAGGGATTG+TGG + chr2_4:46683663-46683682 Msa0331200:CDS 50.0%
! GAAAGTATGGAGCGGGAAAC+CGG + chr2_4:46682828-46682847 Msa0331200:CDS 50.0%
GATGTGTGGAGCAAAGGTAG+TGG - chr2_4:46683024-46683043 None:intergenic 50.0%
! TGAGAAGAGTATGGAGAGGG+TGG + chr2_4:46683454-46683473 Msa0331200:intron 50.0%
CCTGTCTCATCTTCGGGAAA+TGG - chr2_4:46683658-46683677 None:intergenic 50.0%
CCATTTCCCGAAGATGAGAC+AGG + chr2_4:46683655-46683674 Msa0331200:CDS 50.0%
CGAAGATGAGACAGGGATTG+TGG + chr2_4:46683663-46683682 Msa0331200:CDS 50.0%
GTAGCCCACTTGGGAGACTT+TGG + chr2_4:46683203-46683222 Msa0331200:intron 55.0%
GCAAGCAACCGATCCTAGCA+TGG - chr2_4:46683817-46683836 None:intergenic 55.0%
GTAGCCCACTTGGGAGACTT+TGG + chr2_4:46683203-46683222 Msa0331200:intron 55.0%
GCAAGCAACCGATCCTAGCA+TGG - chr2_4:46683817-46683836 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 46682541 46683856 46682541 ID=Msa0331200;Name=Msa0331200
chr2_4 mRNA 46682541 46683856 46682541 ID=Msa0331200-mRNA-1;Parent=Msa0331200;Name=Msa0331200-mRNA-1;_AED=0.26;_eAED=0.29;_QI=0|0.5|0.33|0.66|1|1|3|34|258
chr2_4 exon 46682541 46682870 46682541 ID=Msa0331200-mRNA-1:exon:14144;Parent=Msa0331200-mRNA-1
chr2_4 exon 46683235 46683319 46683235 ID=Msa0331200-mRNA-1:exon:14145;Parent=Msa0331200-mRNA-1
chr2_4 exon 46683461 46683856 46683461 ID=Msa0331200-mRNA-1:exon:14146;Parent=Msa0331200-mRNA-1
chr2_4 CDS 46682541 46682870 46682541 ID=Msa0331200-mRNA-1:cds;Parent=Msa0331200-mRNA-1
chr2_4 CDS 46683235 46683319 46683235 ID=Msa0331200-mRNA-1:cds;Parent=Msa0331200-mRNA-1
chr2_4 CDS 46683461 46683822 46683461 ID=Msa0331200-mRNA-1:cds;Parent=Msa0331200-mRNA-1
chr2_4 three_prime_UTR 46683823 46683856 46683823 ID=Msa0331200-mRNA-1:three_prime_utr;Parent=Msa0331200-mRNA-1
Gene Sequence

>Msa0331200

ATGGGAACTAAACTGAAAGGTTTGAGAACTGACAATGGCCTGAAGTTTGTTTCAGAGCAATTCAATGAGTTTTGCAAGTTGAAAGGAATCAAGAGGCATATGACCGTACCTAGAACACCGCAACAATATGGTCTTGTTGAACTCATGAATAGGACCCTTTTGGAGCGAGTGATGTGTATGCTTCTAGGAGTTGGTTTACCTAAGAGTTTCTGGGGTGAAACAATGACAACTGTGGCCTATTTGATCAACAGATGTCCATCAACGGGAATAGACTTCAAGACACCTATGAAAGTATGGAGCGGGAAACCGGCAGACTACTCATCTTTGAAGGCTAATTCATGGAACAAAACATATTCAAGTGAAGATCAAGTTAATTCATCCACAATTAAGGGAACTATAGGATATGTTCCTCCTGAGTATGGAGAGGGTGGCAAAGTATTACCTCATGGCGATATCTACAGTTACGGGATTCTTCTATTAGAAATGCTCACAGTCACAGGAAAGAGACCAACAAATAGCATGTTTTCTGGAAGTCTTAGTCTACATGCATTCTACAAGATGAAAGTTCCAGCAGGAATCTTTGAGATAGTAGATACGCATTTGCTTTTGCCATTTCCCGAAGATGAGACAGGGATTGTGGAAAACAAGATTAGGAATTGTTTAGTAATGTTTGCAATAATTGGAGTTGCATGTTCCGAAGAGTTTCTTGCTCATCGAATGCCGATAAAAGATGTTATAGCAAAGTTGAATGAAATCAAATCAATGTTTCCATGCTAG
Protein sequence

>Msa0331200

MGTKLKGLRTDNGLKFVSEQFNEFCKLKGIKRHMTVPRTPQQYGLVELMNRTLLERVMCMLLGVGLPKSFWGETMTTVAYLINRCPSTGIDFKTPMKVWSGKPADYSSLKANSWNKTYSSEDQVNSSTIKGTIGYVPPEYGEGGKVLPHGDIYSYGILLLEMLTVTGKRPTNSMFSGSLSLHAFYKMKVPAGIFEIVDTHLLLPFPEDETGIVENKIRNCLVMFAIIGVACSEEFLAHRMPIKDVIAKLNEIKSMFPC*