AlfalfaGEDB | Msa0332470

M. sativa cultivar zhongmu-4 / Msa0332470

KEGG: -
GO: GO:0001101 GO:0002218 GO:0002252 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0003674 GO:0003700 GO:0006355 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009627 GO:0009682 GO:0009719 GO:0009725 GO:0009751 GO:0009753 GO:0009755 GO:0009814 GO:0009862 GO:0009863 GO:0009864 GO:0009867 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0014070 GO:0019219 GO:0019222 GO:0023052 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031347 GO:0031349 GO:0032870 GO:0035690 GO:0042221 GO:0042493 GO:0042742 GO:0043207 GO:0045087 GO:0045088 GO:0045089 GO:0045892 GO:0045934 GO:0046677 GO:0048518 GO:0048519 GO:0048523 GO:0048583 GO:0048584 GO:0048831 GO:0050776 GO:0050778 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051093 GO:0051171 GO:0051172 GO:0051239 GO:0051241 GO:0051252 GO:0051253 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071395 GO:0071407 GO:0071446 GO:0071495 GO:0080090 GO:0080134 GO:0098542 GO:0140110 GO:1900055 GO:1900056 GO:1901698 GO:1901700 GO:1901701 GO:1902679 GO:1903506 GO:1903507 GO:1905622 GO:2000024 GO:2000026 GO:2000112 GO:2000113 GO:2001141
PFAM: PF03106.18

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0332470	XP_003596299.1	82.609	92	14	2	1	90	160	251	6.79e-45	157

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0332470	sp\|Q8GWF1\|WRK38_ARATH	47.541	61	28	1	2	62	123	179	9.98e-14	68.2

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0332470	G7IR29	82.609	92	14	2	1	90	160	251	3.24e-45	157

TFs/TRs:

Gene ID	Type	Classification
Msa0332470	TF	WRKY

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0332470	MtrunA17_Chr2g0315361	82.609	92	14	2	1	90	160	251	6.24e-49	157
Msa0332470	MtrunA17_Chr2g0315371	84.146	82	13	0	1	82	153	234	6.69e-46	149
Msa0332470	MtrunA17_Chr2g0315381	73.864	88	23	0	2	89	157	244	4.85e-42	140
Msa0332470	MtrunA17_Chr8g0350621	50.562	89	35	3	2	85	124	208	8.50e-22	86.7
Msa0332470	MtrunA17_Chr7g0244971	48.684	76	37	1	2	77	136	209	2.68e-19	80.5
Msa0332470	MtrunA17_Chr7g0244981	50.000	74	35	1	2	75	135	206	1.04e-18	78.6
Msa0332470	MtrunA17_Chr8g0350631	48.718	78	35	2	10	85	5	79	1.25e-18	75.9
Msa0332470	MtrunA17_Chr8g0334541	51.282	78	26	2	2	75	116	185	2.08e-18	77.8
Msa0332470	MtrunA17_Chr2g0315401	50.000	76	33	2	19	92	21	93	3.87e-16	69.3
Msa0332470	MtrunA17_Chr3g0128671	39.806	103	42	4	2	84	140	242	1.16e-14	67.8
Msa0332470	MtrunA17_Chr2g0315341	47.143	70	32	2	25	92	1	67	4.50e-12	58.2
Msa0332470	MtrunA17_Chr5g0428351	31.915	94	62	1	2	95	196	287	6.26e-12	60.5
Msa0332470	MtrunA17_Chr7g0275531	46.552	58	31	0	2	59	131	188	2.96e-11	58.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0332470	AT5G22570.1	47.541	61	28	1	2	62	123	179	1.01e-14	68.2
Msa0332470	AT5G01900.1	55.102	49	22	0	1	49	122	170	1.72e-14	67.4
Msa0332470	AT1G66560.1	54.545	55	24	1	2	55	116	170	3.12e-14	66.6
Msa0332470	AT1G66550.2	36.634	101	47	3	2	101	116	200	9.10e-14	65.5
Msa0332470	AT1G66550.1	36.634	101	47	3	2	101	121	205	1.10e-13	65.1
Msa0332470	AT1G80590.1	48.980	49	25	0	2	50	98	146	1.93e-12	61.6
Msa0332470	AT1G66600.1	43.478	69	32	2	2	63	116	184	3.28e-12	60.8
Msa0332470	AT3G56400.1	48.936	47	24	0	2	48	133	179	3.36e-11	58.5

Find 20 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTACCATTCAGACTTAAATT+TGG	0.184409	2_4:+49547199	Msa0332470:CDS
AATTAAGTACCTGAAATATT+TGG	0.227860	2_4:-49546777	None:intergenic
TAACAAATGCCAAATATTTC+AGG	0.288144	2_4:+49546768	Msa0332470:CDS
TTGTACAGGACTACATATTA+TGG	0.292618	2_4:+49547155	Msa0332470:CDS
AAACATCAAAATCGCATAAC+TGG	0.325641	2_4:-49547348	None:intergenic
AGTTCTTTGAACATGTTTGA+TGG	0.334201	2_4:-49547117	None:intergenic
ATTGTTATCAGAGGACAAAA+TGG	0.379017	2_4:-49547225	None:intergenic
GATTCTCCGATGCGAACAAT+TGG	0.449986	2_4:-49547324	None:intergenic
ATTCCAAATTTAAGTCTGAA+TGG	0.467467	2_4:-49547202	None:intergenic
ATGTATGCAGGAATTACTAT+AGG	0.486763	2_4:+49547063	Msa0332470:intron
TTGGTCAGCGATGGTGGTCG+CGG	0.505780	2_4:-49547305	None:intergenic
GATGGTGGTCGCGGTCGAAA+TGG	0.514056	2_4:-49547296	None:intergenic
CGCTGACCAATTGTTCGCAT+CGG	0.547259	2_4:+49547318	Msa0332470:CDS
GAACAATTGGTCAGCGATGG+TGG	0.548372	2_4:-49547311	None:intergenic
AAGAGAACCCTCCTTTGTAC+AGG	0.557218	2_4:+49547141	Msa0332470:CDS
TGCGAACAATTGGTCAGCGA+TGG	0.580574	2_4:-49547314	None:intergenic
ATGTAGTCCTGTACAAAGGA+GGG	0.583465	2_4:-49547148	None:intergenic
TATGTAGTCCTGTACAAAGG+AGG	0.603277	2_4:-49547149	None:intergenic
TAATATAGAATTGTTATCAG+AGG	0.636655	2_4:-49547234	None:intergenic
TAATATGTAGTCCTGTACAA+AGG	0.665931	2_4:-49547152	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTTATAATTCAAAACTTAAT+TGG	+	chr2_4:49547036-49547055	Msa0332470:intron	10.0%
!!	AATTAAGTACCTGAAATATT+TGG	-	chr2_4:49546780-49546799	None:intergenic	20.0%
!!	ATTACAATTATATCTAAGAG+TGG	-	chr2_4:49546958-49546977	None:intergenic	20.0%
!!	TAATATAGAATTGTTATCAG+AGG	-	chr2_4:49547237-49547256	None:intergenic	20.0%
!	TAACAAATGCCAAATATTTC+AGG	+	chr2_4:49546768-49546787	Msa0332470:CDS	25.0%
!	TTGGAACAATAGTCAAAATT+AGG	+	chr2_4:49546857-49546876	Msa0332470:intron	25.0%
!!	TTAATTGGTAATATGTATGC+AGG	+	chr2_4:49547051-49547070	Msa0332470:intron	25.0%
!	TTACCATTCAGACTTAAATT+TGG	+	chr2_4:49547199-49547218	Msa0332470:CDS	25.0%
!	ATTCCAAATTTAAGTCTGAA+TGG	-	chr2_4:49547205-49547224	None:intergenic	25.0%
	ATGTATGCAGGAATTACTAT+AGG	+	chr2_4:49547063-49547082	Msa0332470:intron	30.0%
	AGTTCTTTGAACATGTTTGA+TGG	-	chr2_4:49547120-49547139	None:intergenic	30.0%
	TAATATGTAGTCCTGTACAA+AGG	-	chr2_4:49547155-49547174	None:intergenic	30.0%
	TTGTACAGGACTACATATTA+TGG	+	chr2_4:49547155-49547174	Msa0332470:CDS	30.0%
	ATTGTTATCAGAGGACAAAA+TGG	-	chr2_4:49547228-49547247	None:intergenic	30.0%
	AAACATCAAAATCGCATAAC+TGG	-	chr2_4:49547351-49547370	None:intergenic	30.0%
	GACTAATCTTGATTCTCAAG+TGG	-	chr2_4:49546822-49546841	None:intergenic	35.0%
	AGTCAACATCATGTACTTGT+TGG	+	chr2_4:49546838-49546857	Msa0332470:intron	35.0%
!	AAGAGCTTTTGCAAATGTGA+TGG	-	chr2_4:49547181-49547200	None:intergenic	35.0%
	GATTCTCAAGTGGATGTAGA+TGG	-	chr2_4:49546812-49546831	None:intergenic	40.0%
	TCATCGAAGAGTCGTAGATT+AGG	+	chr2_4:49546896-49546915	Msa0332470:intron	40.0%
	ATGTAGTCCTGTACAAAGGA+GGG	-	chr2_4:49547151-49547170	None:intergenic	40.0%
	TATGTAGTCCTGTACAAAGG+AGG	-	chr2_4:49547152-49547171	None:intergenic	40.0%
	AAGAGAACCCTCCTTTGTAC+AGG	+	chr2_4:49547141-49547160	Msa0332470:CDS	45.0%
	GATTCTCCGATGCGAACAAT+TGG	-	chr2_4:49547327-49547346	None:intergenic	45.0%
	GAACAATTGGTCAGCGATGG+TGG	-	chr2_4:49547314-49547333	None:intergenic	50.0%
	TGCGAACAATTGGTCAGCGA+TGG	-	chr2_4:49547317-49547336	None:intergenic	50.0%
	CGCTGACCAATTGTTCGCAT+CGG	+	chr2_4:49547318-49547337	Msa0332470:CDS	50.0%
!	GATGGTGGTCGCGGTCGAAA+TGG	-	chr2_4:49547299-49547318	None:intergenic	60.0%
!	TTGGTCAGCGATGGTGGTCG+CGG	-	chr2_4:49547308-49547327	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2_4	gene	49546764	49547385	49546764	ID=Msa0332470;Name=Msa0332470
chr2_4	mRNA	49546764	49547385	49546764	ID=Msa0332470-mRNA-1;Parent=Msa0332470;Name=Msa0332470-mRNA-1;_AED=0.39;_eAED=0.51;_QI=0\|0\|0\|1\|0\|0\|2\|0\|112
chr2_4	exon	49546764	49546789	49546764	ID=Msa0332470-mRNA-1:exon:14814;Parent=Msa0332470-mRNA-1
chr2_4	exon	49547073	49547385	49547073	ID=Msa0332470-mRNA-1:exon:14815;Parent=Msa0332470-mRNA-1
chr2_4	CDS	49546764	49546789	49546764	ID=Msa0332470-mRNA-1:cds;Parent=Msa0332470-mRNA-1
chr2_4	CDS	49547073	49547385	49547073	ID=Msa0332470-mRNA-1:cds;Parent=Msa0332470-mRNA-1

Gene Sequence	>Msa0332470 ATGATAACAAATGCCAAATATTTCAGGAATTACTATAGGTGCACTCACATGTACGATCAACATTGTGAAGCCATCAAACATGTTCAAAGAACTCAAGAGAACCCTCCTTTGTACAGGACTACATATTATGGCCATCACATTTGCAAAAGCTCTTACCATTCAGACTTAAATTTGGAATCCATTTTGTCCTCTGATAACAATTCTATATTACTTACTTTCGATAACAACATTTCAAGTGAACTTGAATATCCATTTCGACCGCGACCACCATCGCTGACCAATTGTTCGCATCGGAGAATCTCCAGTTATGCGATTTTGATGTTTATTTTGATTATTTAA
Protein sequence	>Msa0332470 MITNAKYFRNYYRCTHMYDQHCEAIKHVQRTQENPPLYRTTYYGHHICKSSYHSDLNLESILSSDNNSILLTFDNNISSELEYPFRPRPPSLTNCSHRRISSYAILMFILII*