AlfalfaGEDB | Msa0334080

M. sativa cultivar zhongmu-4 / Msa0334080

KEGG: -
GO: -
PFAM: PF08241.15

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0334080	XP_013464765.1	99.288	281	2	0	1	281	1	281	0.0	577

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0334080	sp\|Q8RWZ5\|SD25_ARATH	68.590	156	49	0	304	459	619	774	1.11e-64	226

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0334080	MtrunA17_Chr2g0318241	99.288	281	2	0	1	281	1	281	0.0	577
Msa0334080	MtrunA17_Chr2g0314611	80.114	176	34	1	304	479	611	785	2.82e-87	284
Msa0334080	MtrunA17_Chr2g0318171	78.531	177	37	1	304	479	607	783	5.20e-87	283
Msa0334080	MtrunA17_Chr3g0137741	80.128	156	30	1	304	459	618	772	1.32e-75	253
Msa0334080	MtrunA17_Chr1g0149381	63.776	196	50	3	304	479	627	821	1.01e-67	232
Msa0334080	MtrunA17_Chr2g0333091	60.571	175	65	2	304	478	618	788	1.34e-56	201
Msa0334080	MtrunA17_Chr2g0318201	78.947	114	23	1	367	479	1	114	1.38e-55	181
Msa0334080	MtrunA17_Chr6g0467281	50.282	177	84	2	304	476	614	790	1.59e-48	178
Msa0334080	MtrunA17_Chr6g0467301	49.153	177	86	2	304	476	619	795	6.84e-46	171
Msa0334080	MtrunA17_Chr1g0202331	51.235	162	72	3	304	461	660	818	5.04e-42	160

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0334080	AT5G54400.1	73.776	286	69	3	1	281	1	285	2.10e-152	436
Msa0334080	AT3G15530.1	73.684	285	68	4	1	281	1	282	6.44e-133	386
Msa0334080	AT3G15530.2	73.684	285	68	4	1	281	1	282	6.44e-133	386
Msa0334080	AT4G32300.1	68.590	156	49	0	304	459	619	774	1.13e-65	226
Msa0334080	AT1G34300.1	45.304	181	95	3	304	480	609	789	1.38e-42	162
Msa0334080	AT2G19130.1	47.429	175	91	1	288	461	604	778	4.33e-42	160
Msa0334080	AT5G24080.3	51.572	159	75	2	304	461	530	687	1.15e-41	159
Msa0334080	AT5G24080.1	51.572	159	75	2	304	461	530	687	1.15e-41	159
Msa0334080	AT5G24080.2	51.572	159	75	2	304	461	657	814	1.28e-41	159
Msa0334080	AT4G00340.2	49.359	156	70	3	304	451	586	740	1.50e-37	147

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Find 109 sgRNAs with CRISPR-Local

Find 460 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTGATGTATACAGTTA+TGG	0.390256	2_4:+52223025	Msa0334080:CDS
AAAAGTTGTTCAAATGTTAG+AGG	0.428209	2_4:+52223279	Msa0334080:CDS
AAAATCAAGCAACAATAGCA+AGG	0.600503	2_4:+52214818	Msa0334080:CDS
AAACCTAGTCAGCATGTGCA+TGG	0.570512	2_4:+52215623	Msa0334080:CDS
AAACTGATGTATACAGTTAT+GGG	0.426552	2_4:+52223026	Msa0334080:CDS
AAAGTTGTTCAAATGTTAGA+GGG	0.571298	2_4:+52223280	Msa0334080:CDS
AAATCTGATTTCTCTGAAAT+CGG	0.373169	2_4:-52223097	None:intergenic
AAGGTTACAAGAATTAAAGA+TGG	0.541321	2_4:+52215535	Msa0334080:CDS
AATGCCGAAGTTTCGGATTT+TGG	0.182615	2_4:+52222896	Msa0334080:CDS
ACAGAAGACGGCGGAGGCTG+CGG	0.497416	2_4:+52215415	Msa0334080:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAATCAAGCAACAATAGCA+AGG	+	chr2_4:52214818-52214837	Msa0334080:CDS	30.0%
CTTGACATAAGAGGAAAATT+AGG	-	chr2_4:52214878-52214897	None:intergenic	30.0%
TTAAAAAAAGAAGGAAGCTC+CGG	+	chr2_4:52215206-52215225	Msa0334080:CDS	30.0%
TCGAAGTAATTATCAGGAAA+CGG	-	chr2_4:52215347-52215366	None:intergenic	30.0%
ACCATATCATTCTAAACATC+TGG	-	chr2_4:52215826-52215845	None:intergenic	30.0%
GATGTATATTAGTCAGAATC+TGG	+	chr2_4:52215898-52215917	Msa0334080:intron	30.0%
ACAGTCTCAAACTAAATCAA+GGG	-	chr2_4:52216212-52216231	None:intergenic	30.0%
TACAGTCTCAAACTAAATCA+AGG	-	chr2_4:52216213-52216232	None:intergenic	30.0%
GTAAGAAAAGCCATAAATCA+TGG	+	chr2_4:52216594-52216613	Msa0334080:intron	30.0%
ATGACTTTCCATTCTCTAAA+GGG	-	chr2_4:52216918-52216937	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_4	gene	52214813	52223399	52214813	ID=Msa0334080;Name=Msa0334080
chr2_4	mRNA	52214813	52223399	52214813	ID=Msa0334080-mRNA-1;Parent=Msa0334080;Name=Msa0334080-mRNA-1;_AED=0.37;_eAED=0.37;_QI=0\|0\|0\|1\|1\|1\|2\|0\|482
chr2_4	exon	52214813	52215655	52214813	ID=Msa0334080-mRNA-1:exon:15707;Parent=Msa0334080-mRNA-1
chr2_4	exon	52222794	52223399	52222794	ID=Msa0334080-mRNA-1:exon:15708;Parent=Msa0334080-mRNA-1
chr2_4	CDS	52214813	52215655	52214813	ID=Msa0334080-mRNA-1:cds;Parent=Msa0334080-mRNA-1
chr2_4	CDS	52222794	52223399	52222794	ID=Msa0334080-mRNA-1:cds;Parent=Msa0334080-mRNA-1

Gene Sequence

>Msa0334080

ATGGGAAAATCAAGCAACAATAGCAAGGAATGGACGATCTACGGAATGGAGCAATGGCAAACCCTAATTTTCCTCTTATGTCAAGCAATTTTATTCTCAGTGTTATCAGTTCTCTATTTCGTTTACTTCAATCCAATTTGCAGTTTCTTTGAACGAATAATCACCACCGCCGCCGTCGCCAGATTCGCCGCTGGATTCACCGGCTCAGTAACAGCACTCTCTGCTGTATGTCTCTTCTTCGCCGCGGCGAATTTCTTTTACTCCGCCGTTCCACTTCACTACGACATGGCACAGCGGATCGTCTCCGCCGTTCACGACTGGTCTTCGGTAAAACTCGCTCTTGATTTAGGCTGCTGCGGCCGGGGAATCCTCTTAAACGCCGTCGCAACGCAGTTAAAAAAAGAAGGAAGCTCCGGCCGTGTTGTCGGTTTAGACCGATCGAAGCGTACGACACTGTCCACGCTTCGATCGGCTAAAATGGAAGGAGTTGGTGAATACGTCACGTGTCGCGAAGGCGATGCGAGACGCTTACCGTTTCCTGATAATTACTTCGATGTGGTTGTTTCTGGTGTGTTTGTTCATACTGTAGGGAGAGAGTATGGACAGAAGACGGCGGAGGCTGCGGCGGAGAGGATGAGAGCGGTGGCGGAGATGGTGAGAGTGATGAAACCTGGTGGTGTGGGTGTTGTGTGGGACCTAGTGCACGTGCCGGAGTACGTGCTAAGGTTACAAGAATTAAAGATGGAAGATGTTAGAGTTTCAGAACGTGTTACAGCTTTCATGGTTAGTAGTCACATTGTTTCGTTCAGGAAACCTAGTCAGCATGTGCATGGACCCGCTGAGGATCAGCAAAAGGACTCGCTTATCTACATCAAGATTGTGACTCAAAGATTCTTCATTGTGATATATATCAAGCCTGAAAATGTACTTCTAGATGATAATTTCAATGCCGAAGTTTCGGATTTTGGTTTAACCAAGCTTATGAATAGAGAACAAAGCCTTGTTTTTACAGAATTTAGAGGAACTTGTGGCTACCTTGCACCTGAGTGGATAAAAAACCAAGCTATATCAGACAAAACTGATGTATACAGTTATGGGATGATGTTGCTGGAGATAATTAGTGGGAGGAGGAATTATGATTCTGATCCGATTTCAGAGAAATCAGATTTCCCTTGTTTTGCTTTTAAGATGATGGAAGAAGGTGAAGTGAGAGATATCCTTGATTCCGAGTTGAAAATAGATGATGAGAATGATGATAGGGTTCATCGTGCTATAAGTGTAGCATTGTGGTGTATACAGCATGACATGTCTAACAGACCTTCCATGAGAAAAGTTGTTCAAATGTTAGAGGGTCTTTGCACCGTTCCTAAACCACCAAAACCCTTCAATGAAGGACACGCCTCCTCTTCTAATGCTTATCTATCTGCAGTTAGTCTTCTTTCAGTTTGA

Protein sequence

>Msa0334080

MGKSSNNSKEWTIYGMEQWQTLIFLLCQAILFSVLSVLYFVYFNPICSFFERIITTAAVARFAAGFTGSVTALSAVCLFFAAANFFYSAVPLHYDMAQRIVSAVHDWSSVKLALDLGCCGRGILLNAVATQLKKEGSSGRVVGLDRSKRTTLSTLRSAKMEGVGEYVTCREGDARRLPFPDNYFDVVVSGVFVHTVGREYGQKTAEAAAERMRAVAEMVRVMKPGGVGVVWDLVHVPEYVLRLQELKMEDVRVSERVTAFMVSSHIVSFRKPSQHVHGPAEDQQKDSLIYIKIVTQRFFIVIYIKPENVLLDDNFNAEVSDFGLTKLMNREQSLVFTEFRGTCGYLAPEWIKNQAISDKTDVYSYGMMLLEIISGRRNYDSDPISEKSDFPCFAFKMMEEGEVRDILDSELKIDDENDDRVHRAISVALWCIQHDMSNRPSMRKVVQMLEGLCTVPKPPKPFNEGHASSSNAYLSAVSLLSV*