AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0336840

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0336840 MtrunA17_Chr2g0323271 90.129 233 20 2 1 230 1 233 5.19e-122 345
Msa0336840 MtrunA17_Chr2g0280901 48.611 144 47 2 1 144 1 117 4.55e-32 113
Msa0336840 MtrunA17_Chr2g0322971 52.542 59 28 0 3 61 29 87 1.62e-13 65.1
Msa0336840 MtrunA17_Chr4g0024021 50.847 59 29 0 5 63 36 94 9.86e-13 63.2
Msa0336840 MtrunA17_Chr4g0002211 50.000 66 33 0 3 68 6 71 2.21e-12 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0336840 AT4G27310.1 42.775 173 55 3 1 138 1 164 1.66e-36 128
Msa0336840 AT5G54470.1 47.059 187 78 4 2 188 3 168 2.06e-34 122
Msa0336840 AT3G21890.1 56.000 50 22 0 5 54 31 80 8.41e-11 58.2

Find 73 sgRNAs with CRISPR-Local

Find 178 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCGTTTAGATCATGACTTTC+AGG 0.284332 2_4:+56380086 Msa0336840:CDS
CGGCGGTATAGCGACGTAAA+TGG 0.305299 2_4:+56380311 Msa0336840:CDS
TTCACTTTCTTCTTGTTGCT+CGG 0.346480 2_4:-56379833 None:intergenic
ATGCTGGGACTGCGATTCTA+AGG 0.346631 2_4:+56379630 Msa0336840:CDS
TTCTTCTTGTTGCTCGGCGC+TGG 0.356031 2_4:-56379827 None:intergenic
AATTACAATTAATAATCATA+TGG 0.375265 2_4:-56380647 None:intergenic
GTGGATGAGGTTGATTTGAT+AGG 0.378300 2_4:+56380254 Msa0336840:CDS
ACGAAACCGCGTTGAGAGAA+AGG 0.398885 2_4:+56379519 Msa0336840:five_prime_UTR
TGAAGAAGATGGTGATAATC+AGG 0.403542 2_4:+56379918 Msa0336840:CDS
CGCATCGCCGCCTTTGACGA+CGG 0.405274 2_4:-56380290 None:intergenic
TTCCTCATCGGAATCGTAAT+CGG 0.408569 2_4:-56379881 None:intergenic
GATTATGGTTGTGGCCTTGT+GGG 0.422309 2_4:+56380515 Msa0336840:three_prime_UTR
CTCGGCGCTGGCACCGGCGT+TGG 0.429781 2_4:-56379815 None:intergenic
TTTCTTGTGATGGTTTGGTA+TGG 0.436961 2_4:-56379416 None:intergenic
CGTGGAAAGCCTCCGGCGCT+AGG 0.440306 2_4:+56379725 Msa0336840:CDS
TTGTTGCTCGGCGCTGGCAC+CGG 0.453269 2_4:-56379821 None:intergenic
TGTTTATATAACTTAGGTAA+AGG 0.465810 2_4:+56380587 Msa0336840:three_prime_UTR
CCGACCAGGCTAGCTTATGC+TGG 0.471766 2_4:+56379614 Msa0336840:CDS
GGTTTGGTATGGATGAAGAA+TGG 0.486262 2_4:-56379405 None:intergenic
GTTTGGTATGGATGAAGAAT+GGG 0.493335 2_4:-56379404 None:intergenic
CGCGTTACCGAGCCTAGCGC+CGG 0.498391 2_4:-56379737 None:intergenic
GCCAACGCTAACTCCAACGC+CGG 0.517678 2_4:+56379802 Msa0336840:CDS
ATAATATCTCTAATATAGTA+CGG 0.521383 2_4:-56380364 None:intergenic
CTCGTCCTCATCTTCCTCAT+CGG 0.523137 2_4:-56379893 None:intergenic
CCCACGCCGTGGAAAGCCTC+CGG 0.526413 2_4:+56379718 Msa0336840:CDS
GACTCACAGAAAGTTCTAGC+TGG 0.526494 2_4:-56379592 None:intergenic
AGTCCCAGCATAAGCTAGCC+TGG 0.526991 2_4:-56379618 None:intergenic
ACCGGCGTTGGAGTTAGCGT+TGG 0.527253 2_4:-56379803 None:intergenic
GAGGACGAGGATGAAGAAGA+TGG 0.529527 2_4:+56379907 Msa0336840:CDS
AACTTTCTGTGAGTCCGACC+AGG 0.531925 2_4:+56379600 Msa0336840:CDS
CCACCGGCTTCTAGTTCCTC+CGG 0.538845 2_4:+56379973 Msa0336840:CDS
TGGATGAGGTTGATTTGATA+GGG 0.541461 2_4:+56380255 Msa0336840:CDS
GCCGGAGGCTTTCCACGGCG+TGG 0.543692 2_4:-56379719 None:intergenic
CGATGAGGAAGATGAGGACG+AGG 0.544127 2_4:+56379894 Msa0336840:CDS
CGACGAGGAACCGTCGTCAA+AGG 0.547277 2_4:+56380280 Msa0336840:CDS
TTCCTCCGGTAGCGAAGAGT+CGG 0.547879 2_4:+56379987 Msa0336840:CDS
CGTCAAAGGCGGCGATGCGG+CGG 0.550222 2_4:+56380294 Msa0336840:CDS
AAAGCCTCCGGCGCTAGGCT+CGG 0.551924 2_4:+56379730 Msa0336840:CDS
AACAGCGGCGCAACCACCAC+CGG 0.553693 2_4:+56379957 Msa0336840:CDS
CGACCAGGCTAGCTTATGCT+GGG 0.557426 2_4:+56379615 Msa0336840:CDS
GTTACCGAGCCTAGCGCCGG+AGG 0.559866 2_4:-56379734 None:intergenic
GGATTATGGTTGTGGCCTTG+TGG 0.566677 2_4:+56380514 Msa0336840:three_prime_UTR
CCGGAGGCTTTCCACGGCGT+GGG 0.569147 2_4:-56379718 None:intergenic
CATTCTCAGCCACACGATTG+AGG 0.570452 2_4:+56380341 Msa0336840:CDS
CTAGCGCCGGAGGCTTTCCA+CGG 0.572274 2_4:-56379724 None:intergenic
CGTAACCGACTCTTCGCTAC+CGG 0.573854 2_4:-56379992 None:intergenic
TCAACGAAGAGAGGTGGATG+AGG 0.575020 2_4:+56380241 Msa0336840:CDS
CGTTGGAGTTAGCGTTGGCG+TGG 0.586469 2_4:-56379798 None:intergenic
GATGAAGAATGGGTAAGAAG+AGG 0.601495 2_4:-56379394 None:intergenic
CCAGCATAAGCTAGCCTGGT+CGG 0.605872 2_4:-56379614 None:intergenic
CGGTAGCGAAGAGTCGGTTA+CGG 0.606860 2_4:+56379993 Msa0336840:CDS
TCGTTGTGTGATAGATGCGT+CGG 0.611906 2_4:+56379763 Msa0336840:CDS
CGAGGAACCGTCGTCAAAGG+CGG 0.613549 2_4:+56380283 Msa0336840:CDS
CTACCGGAGGAACTAGAAGC+CGG 0.616312 2_4:-56379976 None:intergenic
CGATTCCGATGAGGAAGATG+AGG 0.616964 2_4:+56379888 Msa0336840:CDS
GAGGAACTAGAAGCCGGTGG+TGG 0.618905 2_4:-56379970 None:intergenic
GAGATTGACATGCGTGACAG+AGG 0.627483 2_4:-56379696 None:intergenic
CGTCGTCAAAGGCGGCGATG+CGG 0.629213 2_4:+56380291 Msa0336840:CDS
GTATAGCGACGTAAATGGAA+CGG 0.632422 2_4:+56380316 Msa0336840:CDS
AACCGACTCTTCGCTACCGG+AGG 0.640395 2_4:-56379989 None:intergenic
CATGTCAATCTCCCACGCCG+TGG 0.644127 2_4:+56379707 Msa0336840:CDS
ATAGTACGGCCTCAATCGTG+TGG 0.647407 2_4:-56380350 None:intergenic
TTGTGTGATAGATGCGTCGG+CGG 0.659823 2_4:+56379766 Msa0336840:CDS
GTGATAATCAGGTTGTGCCG+TGG 0.677277 2_4:+56379929 Msa0336840:CDS
GAGCAACAAGAAGAAAGTGA+AGG 0.680813 2_4:+56379835 Msa0336840:CDS
CACCGATTACGATTCCGATG+AGG 0.696165 2_4:+56379879 Msa0336840:CDS
CCGGAGGAACTAGAAGCCGG+TGG 0.696282 2_4:-56379973 None:intergenic
TGCGCCGCTGTTGATGACCA+CGG 0.697626 2_4:-56379946 None:intergenic
TGATTTGATAGGGTGCGACG+AGG 0.704245 2_4:+56380265 Msa0336840:CDS
TGTGCCGTGGTCATCAACAG+CGG 0.705571 2_4:+56379942 Msa0336840:CDS
GTGCAACGATAACCACGATG+AGG 0.723607 2_4:+56380023 Msa0336840:CDS
GCAAAGCTACTAAACCCACA+AGG 0.727371 2_4:-56380529 None:intergenic
ACTTAGAAATAACCTCATCG+TGG 0.742114 2_4:-56380035 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTACAATTAATAATCATA+TGG - chr2_4:56380650-56380669 None:intergenic 10.0%
!! AATTACAATTAATAATCATA+TGG - chr2_4:56380650-56380669 None:intergenic 10.0%
!! ATAATATCTCTAATATAGTA+CGG - chr2_4:56380367-56380386 None:intergenic 15.0%
!!! TTATTTTATTTGTAATCTCT+TGG + chr2_4:56380383-56380402 Msa0336840:three_prime_UTR 15.0%
!!! TTTTTTGTTTTGATATTTTG+AGG + chr2_4:56380493-56380512 Msa0336840:three_prime_UTR 15.0%
!!! TTTTCTTGTTTATATAACTT+AGG + chr2_4:56380581-56380600 Msa0336840:three_prime_UTR 15.0%
!! ATAATATCTCTAATATAGTA+CGG - chr2_4:56380367-56380386 None:intergenic 15.0%
!!! TTATTTTATTTGTAATCTCT+TGG + chr2_4:56380383-56380402 Msa0336840:three_prime_UTR 15.0%
!!! TTTTTTGTTTTGATATTTTG+AGG + chr2_4:56380493-56380512 Msa0336840:three_prime_UTR 15.0%
!!! TTTTCTTGTTTATATAACTT+AGG + chr2_4:56380581-56380600 Msa0336840:three_prime_UTR 15.0%
!!! ATTTGTGAATTTTGTTTTTG+CGG - chr2_4:56379490-56379509 None:intergenic 20.0%
!!! TTTTTGTTGATTTTGTTTTC+AGG + chr2_4:56380193-56380212 Msa0336840:intron 20.0%
!!! TTTTGATATTTTGAGGATTA+TGG + chr2_4:56380500-56380519 Msa0336840:three_prime_UTR 20.0%
!! TGTTTATATAACTTAGGTAA+AGG + chr2_4:56380587-56380606 Msa0336840:three_prime_UTR 20.0%
!!! ATTTGTGAATTTTGTTTTTG+CGG - chr2_4:56379490-56379509 None:intergenic 20.0%
!!! TTTTTGTTGATTTTGTTTTC+AGG + chr2_4:56380193-56380212 Msa0336840:intron 20.0%
!!! TTTTGATATTTTGAGGATTA+TGG + chr2_4:56380500-56380519 Msa0336840:three_prime_UTR 20.0%
!! TGTTTATATAACTTAGGTAA+AGG + chr2_4:56380587-56380606 Msa0336840:three_prime_UTR 20.0%
!!! TTGATTTTGTTTTCAGGTTA+CGG + chr2_4:56380199-56380218 Msa0336840:intron 25.0%
!!! TTGATTTTGTTTTCAGGTTA+CGG + chr2_4:56380199-56380218 Msa0336840:intron 25.0%
AGGTTACGGATTCAAAAAAT+TGG + chr2_4:56380213-56380232 Msa0336840:intron 30.0%
!! TATTTTGAGGATTATGGTTG+TGG + chr2_4:56380506-56380525 Msa0336840:three_prime_UTR 30.0%
AGGTTACGGATTCAAAAAAT+TGG + chr2_4:56380213-56380232 Msa0336840:intron 30.0%
!! TATTTTGAGGATTATGGTTG+TGG + chr2_4:56380506-56380525 Msa0336840:three_prime_UTR 30.0%
GTTTGGTATGGATGAAGAAT+GGG - chr2_4:56379407-56379426 None:intergenic 35.0%
!! TTTCTTGTGATGGTTTGGTA+TGG - chr2_4:56379419-56379438 None:intergenic 35.0%
! TCTCTTTTCTTGTGATGGTT+TGG - chr2_4:56379424-56379443 None:intergenic 35.0%
! TTCTCTCTCTTTTCTTGTGA+TGG - chr2_4:56379429-56379448 None:intergenic 35.0%
!! TCGCAGTTCTTTTTCATGAT+CGG - chr2_4:56379559-56379578 None:intergenic 35.0%
TTCACTTTCTTCTTGTTGCT+CGG - chr2_4:56379836-56379855 None:intergenic 35.0%
TGAAGAAGATGGTGATAATC+AGG + chr2_4:56379918-56379937 Msa0336840:CDS 35.0%
ACTTAGAAATAACCTCATCG+TGG - chr2_4:56380038-56380057 None:intergenic 35.0%
TCGTTTAGATCATGACTTTC+AGG + chr2_4:56380086-56380105 Msa0336840:CDS 35.0%
TTGGAAAAATCAACGAAGAG+AGG + chr2_4:56380232-56380251 Msa0336840:CDS 35.0%
!! TGGATGAGGTTGATTTGATA+GGG + chr2_4:56380255-56380274 Msa0336840:CDS 35.0%
GTTTGGTATGGATGAAGAAT+GGG - chr2_4:56379407-56379426 None:intergenic 35.0%
!! TTTCTTGTGATGGTTTGGTA+TGG - chr2_4:56379419-56379438 None:intergenic 35.0%
! TCTCTTTTCTTGTGATGGTT+TGG - chr2_4:56379424-56379443 None:intergenic 35.0%
! TTCTCTCTCTTTTCTTGTGA+TGG - chr2_4:56379429-56379448 None:intergenic 35.0%
!! TCGCAGTTCTTTTTCATGAT+CGG - chr2_4:56379559-56379578 None:intergenic 35.0%
TTCACTTTCTTCTTGTTGCT+CGG - chr2_4:56379836-56379855 None:intergenic 35.0%
TGAAGAAGATGGTGATAATC+AGG + chr2_4:56379918-56379937 Msa0336840:CDS 35.0%
ACTTAGAAATAACCTCATCG+TGG - chr2_4:56380038-56380057 None:intergenic 35.0%
TCGTTTAGATCATGACTTTC+AGG + chr2_4:56380086-56380105 Msa0336840:CDS 35.0%
TTGGAAAAATCAACGAAGAG+AGG + chr2_4:56380232-56380251 Msa0336840:CDS 35.0%
!! TGGATGAGGTTGATTTGATA+GGG + chr2_4:56380255-56380274 Msa0336840:CDS 35.0%
GATGAAGAATGGGTAAGAAG+AGG - chr2_4:56379397-56379416 None:intergenic 40.0%
! GGTTTGGTATGGATGAAGAA+TGG - chr2_4:56379408-56379427 None:intergenic 40.0%
! GATTTTCCTTTCTCTCAACG+CGG - chr2_4:56379528-56379547 None:intergenic 40.0%
GAGCAACAAGAAGAAAGTGA+AGG + chr2_4:56379835-56379854 Msa0336840:CDS 40.0%
TTCCTCATCGGAATCGTAAT+CGG - chr2_4:56379884-56379903 None:intergenic 40.0%
GAAAAATCAACGAAGAGAGG+TGG + chr2_4:56380235-56380254 Msa0336840:CDS 40.0%
!! GTGGATGAGGTTGATTTGAT+AGG + chr2_4:56380254-56380273 Msa0336840:CDS 40.0%
GTATAGCGACGTAAATGGAA+CGG + chr2_4:56380316-56380335 Msa0336840:CDS 40.0%
GATGAAGAATGGGTAAGAAG+AGG - chr2_4:56379397-56379416 None:intergenic 40.0%
! GGTTTGGTATGGATGAAGAA+TGG - chr2_4:56379408-56379427 None:intergenic 40.0%
! GATTTTCCTTTCTCTCAACG+CGG - chr2_4:56379528-56379547 None:intergenic 40.0%
GAGCAACAAGAAGAAAGTGA+AGG + chr2_4:56379835-56379854 Msa0336840:CDS 40.0%
TTCCTCATCGGAATCGTAAT+CGG - chr2_4:56379884-56379903 None:intergenic 40.0%
GAAAAATCAACGAAGAGAGG+TGG + chr2_4:56380235-56380254 Msa0336840:CDS 40.0%
!! GTGGATGAGGTTGATTTGAT+AGG + chr2_4:56380254-56380273 Msa0336840:CDS 40.0%
GTATAGCGACGTAAATGGAA+CGG + chr2_4:56380316-56380335 Msa0336840:CDS 40.0%
! GACTCACAGAAAGTTCTAGC+TGG - chr2_4:56379595-56379614 None:intergenic 45.0%
! GGTTCACGCTGCTAACTTTT+TGG + chr2_4:56379651-56379670 Msa0336840:CDS 45.0%
TCGTTGTGTGATAGATGCGT+CGG + chr2_4:56379763-56379782 Msa0336840:CDS 45.0%
!! GATTATGGTTGTGGCCTTGT+GGG + chr2_4:56380515-56380534 Msa0336840:three_prime_UTR 45.0%
GCAAAGCTACTAAACCCACA+AGG - chr2_4:56380532-56380551 None:intergenic 45.0%
! GACTCACAGAAAGTTCTAGC+TGG - chr2_4:56379595-56379614 None:intergenic 45.0%
! GGTTCACGCTGCTAACTTTT+TGG + chr2_4:56379651-56379670 Msa0336840:CDS 45.0%
TCGTTGTGTGATAGATGCGT+CGG + chr2_4:56379763-56379782 Msa0336840:CDS 45.0%
!! GATTATGGTTGTGGCCTTGT+GGG + chr2_4:56380515-56380534 Msa0336840:three_prime_UTR 45.0%
GCAAAGCTACTAAACCCACA+AGG - chr2_4:56380532-56380551 None:intergenic 45.0%
ACGAAACCGCGTTGAGAGAA+AGG + chr2_4:56379519-56379538 Msa0336840:five_prime_UTR 50.0%
AACTTTCTGTGAGTCCGACC+AGG + chr2_4:56379600-56379619 Msa0336840:CDS 50.0%
ATGCTGGGACTGCGATTCTA+AGG + chr2_4:56379630-56379649 Msa0336840:CDS 50.0%
GAGATTGACATGCGTGACAG+AGG - chr2_4:56379699-56379718 None:intergenic 50.0%
TTGTGTGATAGATGCGTCGG+CGG + chr2_4:56379766-56379785 Msa0336840:CDS 50.0%
! CACCGATTACGATTCCGATG+AGG + chr2_4:56379879-56379898 Msa0336840:CDS 50.0%
CGATTCCGATGAGGAAGATG+AGG + chr2_4:56379888-56379907 Msa0336840:CDS 50.0%
CTCGTCCTCATCTTCCTCAT+CGG - chr2_4:56379896-56379915 None:intergenic 50.0%
GAGGACGAGGATGAAGAAGA+TGG + chr2_4:56379907-56379926 Msa0336840:CDS 50.0%
! GTGATAATCAGGTTGTGCCG+TGG + chr2_4:56379929-56379948 Msa0336840:CDS 50.0%
GTGCAACGATAACCACGATG+AGG + chr2_4:56380023-56380042 Msa0336840:CDS 50.0%
TCAACGAAGAGAGGTGGATG+AGG + chr2_4:56380241-56380260 Msa0336840:CDS 50.0%
TGATTTGATAGGGTGCGACG+AGG + chr2_4:56380265-56380284 Msa0336840:CDS 50.0%
CATTCTCAGCCACACGATTG+AGG + chr2_4:56380341-56380360 Msa0336840:CDS 50.0%
ATAGTACGGCCTCAATCGTG+TGG - chr2_4:56380353-56380372 None:intergenic 50.0%
! GGATTATGGTTGTGGCCTTG+TGG + chr2_4:56380514-56380533 Msa0336840:three_prime_UTR 50.0%
ACGAAACCGCGTTGAGAGAA+AGG + chr2_4:56379519-56379538 Msa0336840:five_prime_UTR 50.0%
AACTTTCTGTGAGTCCGACC+AGG + chr2_4:56379600-56379619 Msa0336840:CDS 50.0%
ATGCTGGGACTGCGATTCTA+AGG + chr2_4:56379630-56379649 Msa0336840:CDS 50.0%
GAGATTGACATGCGTGACAG+AGG - chr2_4:56379699-56379718 None:intergenic 50.0%
TTGTGTGATAGATGCGTCGG+CGG + chr2_4:56379766-56379785 Msa0336840:CDS 50.0%
! CACCGATTACGATTCCGATG+AGG + chr2_4:56379879-56379898 Msa0336840:CDS 50.0%
CGATTCCGATGAGGAAGATG+AGG + chr2_4:56379888-56379907 Msa0336840:CDS 50.0%
CTCGTCCTCATCTTCCTCAT+CGG - chr2_4:56379896-56379915 None:intergenic 50.0%
GAGGACGAGGATGAAGAAGA+TGG + chr2_4:56379907-56379926 Msa0336840:CDS 50.0%
! GTGATAATCAGGTTGTGCCG+TGG + chr2_4:56379929-56379948 Msa0336840:CDS 50.0%
GTGCAACGATAACCACGATG+AGG + chr2_4:56380023-56380042 Msa0336840:CDS 50.0%
TCAACGAAGAGAGGTGGATG+AGG + chr2_4:56380241-56380260 Msa0336840:CDS 50.0%
TGATTTGATAGGGTGCGACG+AGG + chr2_4:56380265-56380284 Msa0336840:CDS 50.0%
CATTCTCAGCCACACGATTG+AGG + chr2_4:56380341-56380360 Msa0336840:CDS 50.0%
ATAGTACGGCCTCAATCGTG+TGG - chr2_4:56380353-56380372 None:intergenic 50.0%
! GGATTATGGTTGTGGCCTTG+TGG + chr2_4:56380514-56380533 Msa0336840:three_prime_UTR 50.0%
! CCAGCATAAGCTAGCCTGGT+CGG - chr2_4:56379617-56379636 None:intergenic 55.0%
!! CGACCAGGCTAGCTTATGCT+GGG + chr2_4:56379615-56379634 Msa0336840:CDS 55.0%
AGTCCCAGCATAAGCTAGCC+TGG - chr2_4:56379621-56379640 None:intergenic 55.0%
TTCTTCTTGTTGCTCGGCGC+TGG - chr2_4:56379830-56379849 None:intergenic 55.0%
CGATGAGGAAGATGAGGACG+AGG + chr2_4:56379894-56379913 Msa0336840:CDS 55.0%
TGTGCCGTGGTCATCAACAG+CGG + chr2_4:56379942-56379961 Msa0336840:CDS 55.0%
CTACCGGAGGAACTAGAAGC+CGG - chr2_4:56379979-56379998 None:intergenic 55.0%
TTCCTCCGGTAGCGAAGAGT+CGG + chr2_4:56379987-56380006 Msa0336840:CDS 55.0%
! CGTAACCGACTCTTCGCTAC+CGG - chr2_4:56379995-56380014 None:intergenic 55.0%
CGGTAGCGAAGAGTCGGTTA+CGG + chr2_4:56379993-56380012 Msa0336840:CDS 55.0%
CGGCGGTATAGCGACGTAAA+TGG + chr2_4:56380311-56380330 Msa0336840:CDS 55.0%
! CCAGCATAAGCTAGCCTGGT+CGG - chr2_4:56379617-56379636 None:intergenic 55.0%
!! CGACCAGGCTAGCTTATGCT+GGG + chr2_4:56379615-56379634 Msa0336840:CDS 55.0%
AGTCCCAGCATAAGCTAGCC+TGG - chr2_4:56379621-56379640 None:intergenic 55.0%
TTCTTCTTGTTGCTCGGCGC+TGG - chr2_4:56379830-56379849 None:intergenic 55.0%
CGATGAGGAAGATGAGGACG+AGG + chr2_4:56379894-56379913 Msa0336840:CDS 55.0%
TGTGCCGTGGTCATCAACAG+CGG + chr2_4:56379942-56379961 Msa0336840:CDS 55.0%
CTACCGGAGGAACTAGAAGC+CGG - chr2_4:56379979-56379998 None:intergenic 55.0%
TTCCTCCGGTAGCGAAGAGT+CGG + chr2_4:56379987-56380006 Msa0336840:CDS 55.0%
! CGTAACCGACTCTTCGCTAC+CGG - chr2_4:56379995-56380014 None:intergenic 55.0%
CGGTAGCGAAGAGTCGGTTA+CGG + chr2_4:56379993-56380012 Msa0336840:CDS 55.0%
CGGCGGTATAGCGACGTAAA+TGG + chr2_4:56380311-56380330 Msa0336840:CDS 55.0%
!! CCGACCAGGCTAGCTTATGC+TGG + chr2_4:56379614-56379633 Msa0336840:CDS 60.0%
CATGTCAATCTCCCACGCCG+TGG + chr2_4:56379707-56379726 Msa0336840:CDS 60.0%
!! CGTTGGAGTTAGCGTTGGCG+TGG - chr2_4:56379801-56379820 None:intergenic 60.0%
GCCAACGCTAACTCCAACGC+CGG + chr2_4:56379802-56379821 Msa0336840:CDS 60.0%
! ACCGGCGTTGGAGTTAGCGT+TGG - chr2_4:56379806-56379825 None:intergenic 60.0%
!! TGCGCCGCTGTTGATGACCA+CGG - chr2_4:56379949-56379968 None:intergenic 60.0%
GAGGAACTAGAAGCCGGTGG+TGG - chr2_4:56379973-56379992 None:intergenic 60.0%
!! CCACCGGCTTCTAGTTCCTC+CGG + chr2_4:56379973-56379992 Msa0336840:CDS 60.0%
! AACCGACTCTTCGCTACCGG+AGG - chr2_4:56379992-56380011 None:intergenic 60.0%
CGACGAGGAACCGTCGTCAA+AGG + chr2_4:56380280-56380299 Msa0336840:CDS 60.0%
CGAGGAACCGTCGTCAAAGG+CGG + chr2_4:56380283-56380302 Msa0336840:CDS 60.0%
!! CCGACCAGGCTAGCTTATGC+TGG + chr2_4:56379614-56379633 Msa0336840:CDS 60.0%
CATGTCAATCTCCCACGCCG+TGG + chr2_4:56379707-56379726 Msa0336840:CDS 60.0%
!! CGTTGGAGTTAGCGTTGGCG+TGG - chr2_4:56379801-56379820 None:intergenic 60.0%
GCCAACGCTAACTCCAACGC+CGG + chr2_4:56379802-56379821 Msa0336840:CDS 60.0%
! ACCGGCGTTGGAGTTAGCGT+TGG - chr2_4:56379806-56379825 None:intergenic 60.0%
!! TGCGCCGCTGTTGATGACCA+CGG - chr2_4:56379949-56379968 None:intergenic 60.0%
GAGGAACTAGAAGCCGGTGG+TGG - chr2_4:56379973-56379992 None:intergenic 60.0%
!! CCACCGGCTTCTAGTTCCTC+CGG + chr2_4:56379973-56379992 Msa0336840:CDS 60.0%
! AACCGACTCTTCGCTACCGG+AGG - chr2_4:56379992-56380011 None:intergenic 60.0%
CGACGAGGAACCGTCGTCAA+AGG + chr2_4:56380280-56380299 Msa0336840:CDS 60.0%
CGAGGAACCGTCGTCAAAGG+CGG + chr2_4:56380283-56380302 Msa0336840:CDS 60.0%
! CTAGCGCCGGAGGCTTTCCA+CGG - chr2_4:56379727-56379746 None:intergenic 65.0%
AAAGCCTCCGGCGCTAGGCT+CGG + chr2_4:56379730-56379749 Msa0336840:CDS 65.0%
!! TTGTTGCTCGGCGCTGGCAC+CGG - chr2_4:56379824-56379843 None:intergenic 65.0%
AACAGCGGCGCAACCACCAC+CGG + chr2_4:56379957-56379976 Msa0336840:CDS 65.0%
CCGGAGGAACTAGAAGCCGG+TGG - chr2_4:56379976-56379995 None:intergenic 65.0%
CGCATCGCCGCCTTTGACGA+CGG - chr2_4:56380293-56380312 None:intergenic 65.0%
CGTCGTCAAAGGCGGCGATG+CGG + chr2_4:56380291-56380310 Msa0336840:CDS 65.0%
! CTAGCGCCGGAGGCTTTCCA+CGG - chr2_4:56379727-56379746 None:intergenic 65.0%
AAAGCCTCCGGCGCTAGGCT+CGG + chr2_4:56379730-56379749 Msa0336840:CDS 65.0%
!! TTGTTGCTCGGCGCTGGCAC+CGG - chr2_4:56379824-56379843 None:intergenic 65.0%
AACAGCGGCGCAACCACCAC+CGG + chr2_4:56379957-56379976 Msa0336840:CDS 65.0%
CCGGAGGAACTAGAAGCCGG+TGG - chr2_4:56379976-56379995 None:intergenic 65.0%
CGCATCGCCGCCTTTGACGA+CGG - chr2_4:56380293-56380312 None:intergenic 65.0%
CGTCGTCAAAGGCGGCGATG+CGG + chr2_4:56380291-56380310 Msa0336840:CDS 65.0%
CCGGAGGCTTTCCACGGCGT+GGG - chr2_4:56379721-56379740 None:intergenic 70.0%
CCCACGCCGTGGAAAGCCTC+CGG + chr2_4:56379718-56379737 Msa0336840:CDS 70.0%
CGTGGAAAGCCTCCGGCGCT+AGG + chr2_4:56379725-56379744 Msa0336840:CDS 70.0%
! GTTACCGAGCCTAGCGCCGG+AGG - chr2_4:56379737-56379756 None:intergenic 70.0%
CGCGTTACCGAGCCTAGCGC+CGG - chr2_4:56379740-56379759 None:intergenic 70.0%
CGTCAAAGGCGGCGATGCGG+CGG + chr2_4:56380294-56380313 Msa0336840:CDS 70.0%
CCGGAGGCTTTCCACGGCGT+GGG - chr2_4:56379721-56379740 None:intergenic 70.0%
CCCACGCCGTGGAAAGCCTC+CGG + chr2_4:56379718-56379737 Msa0336840:CDS 70.0%
CGTGGAAAGCCTCCGGCGCT+AGG + chr2_4:56379725-56379744 Msa0336840:CDS 70.0%
! GTTACCGAGCCTAGCGCCGG+AGG - chr2_4:56379737-56379756 None:intergenic 70.0%
CGCGTTACCGAGCCTAGCGC+CGG - chr2_4:56379740-56379759 None:intergenic 70.0%
CGTCAAAGGCGGCGATGCGG+CGG + chr2_4:56380294-56380313 Msa0336840:CDS 70.0%
GCCGGAGGCTTTCCACGGCG+TGG - chr2_4:56379722-56379741 None:intergenic 75.0%
GCCGGAGGCTTTCCACGGCG+TGG - chr2_4:56379722-56379741 None:intergenic 75.0%
!!! CTCGGCGCTGGCACCGGCGT+TGG - chr2_4:56379818-56379837 None:intergenic 80.0%
!!! CTCGGCGCTGGCACCGGCGT+TGG - chr2_4:56379818-56379837 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 56379389 56380703 56379389 ID=Msa0336840;Name=Msa0336840
chr2_4 mRNA 56379389 56380703 56379389 ID=Msa0336840-mRNA-1;Parent=Msa0336840;Name=Msa0336840-mRNA-1;_AED=0.00;_eAED=0.00;_QI=173|1|1|1|1|1|2|342|230
chr2_4 exon 56379389 56380107 56379389 ID=Msa0336840-mRNA-1:exon:17173;Parent=Msa0336840-mRNA-1
chr2_4 exon 56380215 56380703 56380215 ID=Msa0336840-mRNA-1:exon:17174;Parent=Msa0336840-mRNA-1
chr2_4 five_prime_UTR 56379389 56379561 56379389 ID=Msa0336840-mRNA-1:five_prime_utr;Parent=Msa0336840-mRNA-1
chr2_4 CDS 56379562 56380107 56379562 ID=Msa0336840-mRNA-1:cds;Parent=Msa0336840-mRNA-1
chr2_4 CDS 56380215 56380361 56380215 ID=Msa0336840-mRNA-1:cds;Parent=Msa0336840-mRNA-1
chr2_4 three_prime_UTR 56380362 56380703 56380362 ID=Msa0336840-mRNA-1:three_prime_utr;Parent=Msa0336840-mRNA-1
Gene Sequence

>Msa0336840

ATGAAAAAGAACTGCGAACTCTGCAAGCTTCCAGCTAGAACTTTCTGTGAGTCCGACCAGGCTAGCTTATGCTGGGACTGCGATTCTAAGGTTCACGCTGCTAACTTTTTGGTTGAGAGACACATGAGAACTCTCCTCTGTCACGCATGTCAATCTCCCACGCCGTGGAAAGCCTCCGGCGCTAGGCTCGGTAACGCGCTTTCGTTGTGTGATAGATGCGTCGGCGGAAGAAAACTCCACGCCAACGCTAACTCCAACGCCGGTGCCAGCGCCGAGCAACAAGAAGAAAGTGAAGGTGACAATGACAATGAAGAAGACACCGATTACGATTCCGATGAGGAAGATGAGGACGAGGATGAAGAAGATGGTGATAATCAGGTTGTGCCGTGGTCATCAACAGCGGCGCAACCACCACCGGCTTCTAGTTCCTCCGGTAGCGAAGAGTCGGTTACGGTTAGCAAGTGCAACGATAACCACGATGAGGTTATTTCTAAGTTAGTTACAACGATTTCTTTGAAACGACGTCGTTTAGATCATGACTTTCAGGTTACGGATTCAAAAAATTGGAAAAATCAACGAAGAGAGGTGGATGAGGTTGATTTGATAGGGTGCGACGAGGAACCGTCGTCAAAGGCGGCGATGCGGCGGTATAGCGACGTAAATGGAACGGAGCATTCTCAGCCACACGATTGA
Protein sequence

>Msa0336840

MKKNCELCKLPARTFCESDQASLCWDCDSKVHAANFLVERHMRTLLCHACQSPTPWKASGARLGNALSLCDRCVGGRKLHANANSNAGASAEQQEESEGDNDNEEDTDYDSDEEDEDEDEEDGDNQVVPWSSTAAQPPPASSSSGSEESVTVSKCNDNHDEVISKLVTTISLKRRRLDHDFQVTDSKNWKNQRREVDEVDLIGCDEEPSSKAAMRRYSDVNGTEHSQPHD*