AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0338790

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0338790 MtrunA17_Chr2g0288601 89.362 94 10 0 12 105 29 122 3.11e-58 181
Msa0338790 MtrunA17_Chr7g0243081 82.609 69 12 0 42 110 59 127 5.85e-35 127
Msa0338790 MtrunA17_Chr1g0210911 79.412 68 14 0 45 112 13 80 3.85e-34 125
Msa0338790 MtrunA17_Chr1g0162931 78.788 66 14 0 45 110 9 74 2.95e-33 121
Msa0338790 MtrunA17_Chr2g0320231 66.265 83 28 0 42 124 85 167 4.79e-33 123
Msa0338790 MtrunA17_Chr8g0379121 80.000 65 13 0 46 110 103 167 4.48e-32 121
Msa0338790 MtrunA17_Chr2g0283951 68.493 73 19 1 46 114 81 153 3.10e-27 107
Msa0338790 MtrunA17_Chr3g0095611 55.714 70 31 0 46 115 11 80 3.49e-23 93.2
Msa0338790 MtrunA17_Chr1g0154261 65.672 67 22 1 45 110 116 182 8.93e-22 91.3
Msa0338790 MtrunA17_Chr7g0261911 43.243 111 57 2 28 132 231 341 3.10e-21 91.7
Msa0338790 MtrunA17_Chr6g0479431 57.143 70 30 0 53 122 201 270 6.11e-21 90.1
Msa0338790 MtrunA17_Chr8g0366281 67.241 58 19 0 53 110 172 229 1.09e-20 89.4
Msa0338790 MtrunA17_Chr5g0410741 58.333 72 29 1 48 118 212 283 2.37e-20 89.0
Msa0338790 MtrunA17_Chr1g0190301 64.286 56 20 0 55 110 176 231 5.43e-20 87.8
Msa0338790 MtrunA17_Chr7g0274471 57.971 69 27 1 55 121 219 287 6.37e-20 87.8
Msa0338790 MtrunA17_Chr3g0109981 64.062 64 22 1 48 110 267 330 6.84e-20 87.8
Msa0338790 MtrunA17_Chr6g0461641 64.151 53 19 0 55 107 43 95 4.27e-18 82.4
Msa0338790 MtrunA17_Chr7g0258551 60.656 61 24 0 50 110 208 268 8.40e-18 81.3
Msa0338790 MtrunA17_Chr1g0197171 63.158 57 21 0 55 111 45 101 1.16e-17 80.1
Msa0338790 MtrunA17_Chr5g0409731 62.069 58 22 0 50 107 21 78 1.18e-17 80.1
Msa0338790 MtrunA17_Chr2g0290991 63.158 57 21 0 55 111 40 96 1.51e-17 80.1
Msa0338790 MtrunA17_Chr7g0254981 45.283 106 55 2 46 149 42 146 1.62e-17 79.3
Msa0338790 MtrunA17_Chr2g0321461 62.264 53 20 0 55 107 46 98 2.89e-17 80.1
Msa0338790 MtrunA17_Chr5g0417521 53.333 60 28 0 55 114 16 75 4.61e-17 79.0
Msa0338790 MtrunA17_Chr1g0175091 59.091 66 25 1 56 121 260 323 8.76e-17 78.6
Msa0338790 MtrunA17_Chr1g0175081 58.182 55 23 0 56 110 24 78 1.78e-16 72.8
Msa0338790 MtrunA17_Chr2g0291021 65.455 55 19 0 56 110 51 105 2.02e-16 77.0
Msa0338790 MtrunA17_Chr2g0310011 45.556 90 42 2 55 138 36 124 2.57e-16 77.0
Msa0338790 MtrunA17_Chr8g0356501 41.489 94 49 1 18 111 7 94 4.43e-16 76.3
Msa0338790 MtrunA17_Chr4g0042451 50.000 64 31 1 55 118 18 80 2.03e-15 74.3
Msa0338790 MtrunA17_Chr4g0045361 50.000 68 34 0 40 107 260 327 1.28e-14 72.4
Msa0338790 MtrunA17_Chr1g0175071 58.491 53 22 0 56 108 179 231 5.93e-14 70.1
Msa0338790 MtrunA17_Chr7g0242521 61.818 55 18 1 56 107 15 69 1.95e-13 67.4
Msa0338790 MtrunA17_Chr4g0062921 57.692 52 22 0 56 107 237 288 7.79e-13 67.4
Msa0338790 MtrunA17_Chr2g0304841 54.545 55 24 1 53 107 212 265 1.56e-12 66.6
Msa0338790 MtrunA17_Chr7g0258531 35.556 135 64 4 14 132 171 298 2.45e-12 65.5
Msa0338790 MtrunA17_Chr4g0031961 47.126 87 42 4 22 107 100 183 8.50e-12 63.5
Msa0338790 MtrunA17_Chr7g0275401 54.545 55 24 1 53 107 191 244 2.40e-11 62.4
Msa0338790 MtrunA17_Chr1g0198601 51.923 52 25 0 56 107 213 264 4.91e-11 61.6
Msa0338790 MtrunA17_Chr7g0255711 38.000 100 53 3 53 144 90 188 5.95e-11 61.2
Msa0338790 MtrunA17_Chr4g0009901 46.429 56 29 1 52 107 166 220 8.69e-11 60.5
Msa0338790 MtrunA17_Chr5g0422621 50.000 52 26 0 56 107 200 251 9.99e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0338790 AT2G38300.1 56.667 120 48 2 30 145 30 149 1.05e-34 127
Msa0338790 AT4G04605.1 53.636 110 46 2 41 149 1 106 6.39e-34 122
Msa0338790 AT2G02060.2 83.871 62 10 0 46 107 21 82 9.55e-33 119
Msa0338790 AT2G02060.1 83.871 62 10 0 46 107 21 82 9.55e-33 119
Msa0338790 AT2G42660.1 46.809 141 70 2 46 185 42 178 2.87e-31 115
Msa0338790 AT2G40260.1 63.529 85 31 0 33 117 61 145 4.48e-31 118
Msa0338790 AT1G14600.1 79.032 62 13 0 46 107 15 76 1.91e-29 111
Msa0338790 AT4G04555.1 57.534 73 31 0 41 113 1 73 3.50e-25 95.9
Msa0338790 AT4G04580.1 48.864 88 42 1 41 128 1 85 4.79e-24 94.7
Msa0338790 AT4G17695.1 50.526 95 40 1 16 110 133 220 6.72e-23 95.1
Msa0338790 AT5G42630.2 68.966 58 18 0 53 110 104 161 5.66e-22 90.9
Msa0338790 AT5G42630.1 68.966 58 18 0 53 110 104 161 6.63e-22 91.7
Msa0338790 AT1G32240.1 58.333 72 29 1 48 118 206 277 4.23e-20 88.2
Msa0338790 AT1G32240.2 64.062 64 22 1 48 110 206 269 4.64e-20 88.2
Msa0338790 AT5G16560.1 64.062 64 22 1 48 110 212 275 5.59e-20 88.2
Msa0338790 AT4G28610.1 65.455 55 19 0 56 110 227 281 1.05e-18 84.7
Msa0338790 AT5G29000.1 65.455 55 19 0 56 110 190 244 1.13e-18 84.3
Msa0338790 AT5G29000.3 65.455 55 19 0 56 110 190 244 1.13e-18 84.3
Msa0338790 AT5G29000.4 65.455 55 19 0 56 110 233 287 1.18e-18 83.6
Msa0338790 AT5G29000.2 65.455 55 19 0 56 110 233 287 1.37e-18 84.3
Msa0338790 AT3G04450.3 61.818 55 21 0 56 110 240 294 2.10e-18 82.8
Msa0338790 AT3G04450.4 61.818 55 21 0 56 110 200 254 2.24e-18 83.6
Msa0338790 AT3G04450.2 61.818 55 21 0 56 110 200 254 2.24e-18 83.6
Msa0338790 AT3G04450.1 61.818 55 21 0 56 110 240 294 2.74e-18 83.6
Msa0338790 AT5G45580.1 58.730 63 26 0 50 112 3 65 3.54e-18 81.3
Msa0338790 AT5G45580.2 58.730 63 26 0 50 112 19 81 3.77e-18 81.6
Msa0338790 AT3G13040.3 49.383 81 41 0 56 136 243 323 1.24e-17 81.6
Msa0338790 AT3G13040.1 49.383 81 41 0 56 136 243 323 1.24e-17 81.6
Msa0338790 AT3G13040.2 49.383 81 41 0 56 136 243 323 1.24e-17 81.6
Msa0338790 AT2G20400.2 63.636 55 20 0 56 110 233 287 3.00e-17 80.5
Msa0338790 AT2G20400.1 63.636 55 20 0 56 110 233 287 3.01e-17 80.5
Msa0338790 AT3G04030.1 60.377 53 21 0 55 107 46 98 3.50e-17 80.1
Msa0338790 AT5G18240.2 62.264 53 20 0 55 107 46 98 4.13e-17 80.1
Msa0338790 AT5G18240.3 62.264 53 20 0 55 107 46 98 4.13e-17 80.1
Msa0338790 AT3G04030.3 60.377 53 21 0 55 107 46 98 4.34e-17 79.7
Msa0338790 AT5G18240.1 62.264 53 20 0 55 107 46 98 4.81e-17 79.7
Msa0338790 AT5G18240.4 62.264 53 20 0 55 107 46 98 4.81e-17 79.7
Msa0338790 AT5G18240.5 62.264 53 20 0 55 107 46 98 5.01e-17 79.7
Msa0338790 AT3G12730.1 59.649 57 23 0 55 111 24 80 8.07e-17 77.0
Msa0338790 AT2G01060.1 65.455 55 19 0 56 110 17 71 1.19e-16 77.4
Msa0338790 AT2G06020.1 52.542 59 28 0 49 107 81 139 1.84e-16 77.4
Msa0338790 AT5G06800.2 42.727 110 55 3 28 132 169 275 3.10e-16 77.4
Msa0338790 AT5G06800.3 49.398 83 39 2 28 110 169 248 3.15e-16 76.3
Msa0338790 AT4G13640.1 56.140 57 25 0 55 111 38 94 4.31e-16 76.3
Msa0338790 AT4G13640.2 56.140 57 25 0 55 111 38 94 4.32e-16 76.3
Msa0338790 AT3G24120.1 56.140 57 25 0 55 111 42 98 4.54e-16 76.3
Msa0338790 AT3G24120.2 56.140 57 25 0 55 111 42 98 4.78e-16 75.9
Msa0338790 AT1G79430.2 57.895 57 24 0 55 111 35 91 6.39e-16 76.3
Msa0338790 AT5G06800.1 49.398 83 39 2 28 110 169 248 1.50e-15 75.5
Msa0338790 AT1G69580.2 58.491 53 22 0 55 107 35 87 5.57e-15 73.6
Msa0338790 AT1G69580.1 58.491 53 22 0 55 107 35 87 5.59e-15 73.6
Msa0338790 AT3G04030.2 58.491 53 21 1 55 107 46 97 1.43e-14 72.8
Msa0338790 AT3G62670.1 35.849 106 56 3 52 145 209 314 9.93e-14 70.1
Msa0338790 AT2G03500.1 57.692 52 22 0 56 107 235 286 1.11e-12 67.0
Msa0338790 AT5G59570.2 55.357 56 24 1 52 107 139 193 1.83e-12 65.9
Msa0338790 AT5G59570.1 55.357 56 24 1 52 107 139 193 1.83e-12 65.9
Msa0338790 AT5G44190.1 46.269 67 35 1 53 119 146 211 2.06e-12 66.2
Msa0338790 AT1G25550.1 55.769 52 23 0 56 107 211 262 2.38e-12 65.9
Msa0338790 AT5G44190.2 46.269 67 35 1 53 119 146 211 2.78e-12 65.9
Msa0338790 AT4G37180.1 50.847 59 29 0 49 107 205 263 3.25e-12 65.5
Msa0338790 AT4G37180.2 50.847 59 29 0 49 107 212 270 4.14e-12 65.5
Msa0338790 AT3G46640.2 54.545 55 24 1 53 107 143 196 9.14e-12 63.9
Msa0338790 AT3G46640.1 54.545 55 24 1 53 107 143 196 9.14e-12 63.9
Msa0338790 AT2G40970.1 37.500 96 55 2 53 144 104 198 9.19e-12 63.5
Msa0338790 AT3G46640.3 54.545 55 24 1 53 107 143 196 1.36e-11 63.5
Msa0338790 AT1G25550.2 51.923 52 25 0 56 107 211 262 5.95e-11 61.2
Msa0338790 AT1G68670.1 53.846 52 24 0 56 107 217 268 6.43e-11 61.6
Msa0338790 AT4G37180.3 49.123 57 29 0 49 105 205 261 9.70e-11 60.8

Find 55 sgRNAs with CRISPR-Local

Find 184 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTATTCCAGGGATGCTTAT+TGG 0.219949 2_4:+59350227 Msa0338790:intron
GAATCTTTGTTGGTCGCTTT+TGG 0.263083 2_4:-59351490 None:intergenic
GTTTGGAGAATTACATTCTT+TGG 0.276827 2_4:-59350072 None:intergenic
CACACTTACATCCTCTTTGA+TGG 0.313928 2_4:+59350319 Msa0338790:CDS
ATCCTCTTTGATGGTATTCT+CGG 0.352821 2_4:+59350328 Msa0338790:CDS
GGACCTCCTGAGCTTGTTGA+TGG 0.365393 2_4:-59351564 None:intergenic
TTATATCTTCGAATCTTTGT+TGG 0.384293 2_4:-59351500 None:intergenic
ATTCACTCTCTCTCCTCTCC+TGG 0.393108 2_4:-59351523 None:intergenic
ATGAATTGTAAAACTAACTT+TGG 0.405423 2_4:-59348470 None:intergenic
TCATGTCAAAAGCCACCTTC+AGG 0.411764 2_4:+59348517 Msa0338790:CDS
TTTACAATTCATGAATATCA+AGG 0.423774 2_4:+59348481 Msa0338790:CDS
CAATCAATTGTGATCTGTAA+TGG 0.425833 2_4:+59350280 Msa0338790:CDS
TATTGGCTAGAGTCTTTCAA+TGG 0.428858 2_4:+59350244 Msa0338790:CDS
CAATGTTATTGCAGATGTAC+AGG 0.430914 2_4:+59348711 Msa0338790:intron
GACCTCCTGAGCTTGTTGAT+GGG 0.440748 2_4:-59351563 None:intergenic
CAAAGTTGCCTCGGCTTCGT+TGG 0.452111 2_4:+59348157 Msa0338790:CDS
GTGCATGCAGTTCAAAGGCT+TGG 0.455826 2_4:+59348204 Msa0338790:CDS
AAACAAGAGCAGATGATTGA+AGG 0.472078 2_4:+59348740 Msa0338790:CDS
ACAAAGCAACGGTGAAGATC+TGG 0.473150 2_4:-59348183 None:intergenic
TTCTCCTACTAGCGGAGTTG+TGG 0.487718 2_4:+59348023 Msa0338790:CDS
AGAGAGACATCATTTGTGTC+TGG 0.491055 2_4:-59351585 None:intergenic
GGCAACTTTGATCGAACATA+AGG 0.496488 2_4:-59348144 None:intergenic
CTGTCCCCATCAACAAGCTC+AGG 0.503401 2_4:+59351558 Msa0338790:CDS
CCAAGCTACTATCTGAATCA+TGG 0.514407 2_4:+59350049 Msa0338790:CDS
ATGTTCGATCAAAGTTGCCT+CGG 0.517554 2_4:+59348148 Msa0338790:CDS
TTTACCAGCTGCAAAGAGAA+AGG 0.520218 2_4:+59349976 Msa0338790:intron
TTACAATTCATGAATATCAA+GGG 0.527486 2_4:+59348482 Msa0338790:CDS
GCTTTGTGCATGCAGTTCAA+AGG 0.532535 2_4:+59348199 Msa0338790:CDS
ACCTCCTGAGCTTGTTGATG+GGG 0.534570 2_4:-59351562 None:intergenic
CGCACATCAGAAGGTTGTGA+AGG 0.540162 2_4:-59348054 None:intergenic
ATTTACGTACTTGAACAGTC+TGG 0.542233 2_4:-59350354 None:intergenic
CATGCAGTTCAAAGGCTTGG+AGG 0.546546 2_4:+59348207 Msa0338790:CDS
GAGTCTTTCAATGGAACTCA+AGG 0.550479 2_4:+59350253 Msa0338790:CDS
GCTTGGAACAAGGGCTGAGG+AGG 0.553779 2_4:-59350032 None:intergenic
CCATGATTCAGATAGTAGCT+TGG 0.557206 2_4:-59350049 None:intergenic
GTGTCCACAACTCCGCTAGT+AGG 0.568408 2_4:-59348027 None:intergenic
TCTTCTTCTTCTCCTACTAG+CGG 0.575646 2_4:+59348015 Msa0338790:CDS
TACAATTCATGAATATCAAG+GGG 0.575681 2_4:+59348483 Msa0338790:CDS
TGAAAAGTTCGCACATCAGA+AGG 0.579697 2_4:-59348063 None:intergenic
CTCTTTGATGGTATTCTCGG+AGG 0.601250 2_4:+59350331 Msa0338790:CDS
CAGATAGTAGCTTGGAACAA+GGG 0.609739 2_4:-59350041 None:intergenic
TCAGATAGTAGCTTGGAACA+AGG 0.613357 2_4:-59350042 None:intergenic
GTAGCTTGGAACAAGGGCTG+AGG 0.617170 2_4:-59350035 None:intergenic
TCCCCATCAACAAGCTCAGG+AGG 0.619846 2_4:+59351561 Msa0338790:CDS
TTCGAAGATATAACCAGGAG+AGG 0.626534 2_4:+59351510 Msa0338790:CDS
GAACTGCATGCACAAAGCAA+CGG 0.628677 2_4:-59348194 None:intergenic
TCTGGTGTCCAACGAAGCCG+AGG 0.632658 2_4:-59348165 None:intergenic
CGAACATAAGGTCTCACCAC+AGG 0.634731 2_4:-59348132 None:intergenic
GCACATCAGAAGGTTGTGAA+GGG 0.634756 2_4:-59348053 None:intergenic
AGCTGCAAAGAGAAAGGACA+TGG 0.645199 2_4:+59349982 Msa0338790:intron
CTCCGAGAATACCATCAAAG+AGG 0.653875 2_4:-59350330 None:intergenic
GATGAGTCTAAAAGCACCTG+TGG 0.662178 2_4:+59348116 Msa0338790:CDS
AAAGATTCGAAGATATAACC+AGG 0.674187 2_4:+59351505 Msa0338790:CDS
AGAGAAAGAGTACCTGAAGG+TGG 0.689810 2_4:-59348529 None:intergenic
TCTGATGAGAATCTAAACGA+CGG 0.728967 2_4:-59348086 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTGAAATTTCAAAATTTTA+GGG - chr2_4:59348695-59348714 None:intergenic 10.0%
!!! TAAATGTATATTTTTTTAAC+AGG - chr2_4:59349795-59349814 None:intergenic 10.0%
!!! GAAATAAATTTTAATCAATA+AGG - chr2_4:59351353-59351372 None:intergenic 10.0%
!! AATATTGAATAAAAGAACTA+GGG - chr2_4:59348249-59348268 None:intergenic 15.0%
!! TAATATTGAATAAAAGAACT+AGG - chr2_4:59348250-59348269 None:intergenic 15.0%
!!! ATGATTTTTTTAATAGTAGA+TGG - chr2_4:59348370-59348389 None:intergenic 15.0%
!!! TATTAATCAAACATTTTTAG+TGG - chr2_4:59348571-59348590 None:intergenic 15.0%
!! TAAAAAAACGAACATAAATA+AGG - chr2_4:59348633-59348652 None:intergenic 15.0%
!!! ATTTTAGGGAAAAAAAAATT+TGG - chr2_4:59348681-59348700 None:intergenic 15.0%
!!! CATTGAAATTTCAAAATTTT+AGG - chr2_4:59348696-59348715 None:intergenic 15.0%
!! TTTATAAAAGTTTGTGTATT+TGG + chr2_4:59349292-59349311 Msa0338790:intron 15.0%
!! TATTTATATAAACTACTTTG+AGG + chr2_4:59350430-59350449 Msa0338790:intron 15.0%
!! CAAAAAAAAAAACTTACAAA+AGG - chr2_4:59350615-59350634 None:intergenic 15.0%
!!! CTTTTGTAAGTTTTTTTTTT+TGG + chr2_4:59350613-59350632 Msa0338790:intron 15.0%
!! TATGAAATAAACATGTAAAT+AGG - chr2_4:59350674-59350693 None:intergenic 15.0%
!! AAATAAAGGACTATTTAAAT+AGG - chr2_4:59350821-59350840 None:intergenic 15.0%
!!! TAAGGGAAAATATATTTTAT+TGG - chr2_4:59351335-59351354 None:intergenic 15.0%
!!! TGACTTGAAAACATTATTTT+TGG + chr2_4:59348406-59348425 Msa0338790:intron 20.0%
!! ATGAATTGTAAAACTAACTT+TGG - chr2_4:59348473-59348492 None:intergenic 20.0%
!! TTTACAATTCATGAATATCA+AGG + chr2_4:59348481-59348500 Msa0338790:CDS 20.0%
!! TTACAATTCATGAATATCAA+GGG + chr2_4:59348482-59348501 Msa0338790:CDS 20.0%
!! GGTATTACTAATATTAATTG+TGG + chr2_4:59348761-59348780 Msa0338790:intron 20.0%
!! AATACATTAGACATATTGTT+TGG + chr2_4:59348871-59348890 Msa0338790:intron 20.0%
!! AATAGAACAAAACAAGAAAT+AGG + chr2_4:59348960-59348979 Msa0338790:intron 20.0%
!!! ACAAAATGATGTATTTTTCT+AGG - chr2_4:59349069-59349088 None:intergenic 20.0%
!!! AAAAATACATCATTTTGTGT+TGG + chr2_4:59349071-59349090 Msa0338790:intron 20.0%
!!! GAAGTAGAATTTTTTATTAC+AGG + chr2_4:59349185-59349204 Msa0338790:intron 20.0%
!! TCTAAATAGTGAAATTTGAT+TGG + chr2_4:59349470-59349489 Msa0338790:intron 20.0%
!! ACGATAAAAAAAAGAAAGTT+TGG - chr2_4:59350092-59350111 None:intergenic 20.0%
!!! CTTTACAATGCATATAATTT+TGG + chr2_4:59350191-59350210 Msa0338790:intron 20.0%
!!! AAGACTTACAAATTTTTTGT+AGG - chr2_4:59350578-59350597 None:intergenic 20.0%
!!! AATCAAACTTTTAAATAGAC+TGG - chr2_4:59350640-59350659 None:intergenic 20.0%
!! ACTTATTTAAGTAAATAGCT+CGG - chr2_4:59350968-59350987 None:intergenic 20.0%
!! GTTATTAAATGATTGAATGT+TGG - chr2_4:59351080-59351099 None:intergenic 20.0%
!!! TTTTGAATTGATTTTGTCTT+CGG + chr2_4:59351138-59351157 Msa0338790:intron 20.0%
!! ATAAGAGCAAATATGTTAAA+CGG - chr2_4:59351307-59351326 None:intergenic 20.0%
!! TTAACATATTTGCTCTTATT+TGG + chr2_4:59351308-59351327 Msa0338790:intron 20.0%
! TCAAGTCAAATTAACAACAA+AGG - chr2_4:59348394-59348413 None:intergenic 25.0%
! TATATGTATGCTTGTATTAC+AGG + chr2_4:59348440-59348459 Msa0338790:intron 25.0%
! ATATGTATGCTTGTATTACA+GGG + chr2_4:59348441-59348460 Msa0338790:intron 25.0%
! TACAATTCATGAATATCAAG+GGG + chr2_4:59348483-59348502 Msa0338790:CDS 25.0%
! TCTTTGTAAATGGAAGAAAT+AGG + chr2_4:59348839-59348858 Msa0338790:intron 25.0%
!!! ATTTTTCTAGGAACAAAATC+TGG - chr2_4:59349057-59349076 None:intergenic 25.0%
! TTGAGGTCTATAATTTCTTT+GGG - chr2_4:59349148-59349167 None:intergenic 25.0%
! ATTGAGGTCTATAATTTCTT+TGG - chr2_4:59349149-59349168 None:intergenic 25.0%
!!! TACCACGTTTATTTTTATTG+AGG - chr2_4:59349165-59349184 None:intergenic 25.0%
!!! AGTTTTAAAAAAGTGTTCTG+AGG - chr2_4:59349228-59349247 None:intergenic 25.0%
! CATATTCAAAAAACTGTTTC+AGG - chr2_4:59349568-59349587 None:intergenic 25.0%
!! TATGTTCCTCAAACAATTTT+TGG + chr2_4:59349584-59349603 Msa0338790:intron 25.0%
! TAGATGATAACAGATAGATA+GGG - chr2_4:59349625-59349644 None:intergenic 25.0%
!! TACTTTATCAAGTCTTTTTG+TGG + chr2_4:59349686-59349705 Msa0338790:intron 25.0%
!! ATACTTTATCAAGTCTTATC+CGG + chr2_4:59349715-59349734 Msa0338790:intron 25.0%
! TTGAATCACATATCATAATG+TGG - chr2_4:59349843-59349862 None:intergenic 25.0%
! CATTATGATATGTGATTCAA+TGG + chr2_4:59349843-59349862 Msa0338790:intron 25.0%
!!! TTCCTCTCTTCTTTTATTTA+CGG + chr2_4:59350111-59350130 Msa0338790:intron 25.0%
! ATTTCTCTCTTAATTATTCC+AGG + chr2_4:59350215-59350234 Msa0338790:intron 25.0%
! TTTCTCTCTTAATTATTCCA+GGG + chr2_4:59350216-59350235 Msa0338790:intron 25.0%
! CATAGTATTAGTATGTAGAA+AGG - chr2_4:59350399-59350418 None:intergenic 25.0%
! AAAAAAATGTGTTTGTGTGT+TGG - chr2_4:59350480-59350499 None:intergenic 25.0%
!!! TTACAAATTTTTTGTAGGTC+AGG - chr2_4:59350573-59350592 None:intergenic 25.0%
! GACTATTTAAATAGGCTTTA+AGG - chr2_4:59350813-59350832 None:intergenic 25.0%
! TCAAGTAACAACTCAAATAA+AGG - chr2_4:59350835-59350854 None:intergenic 25.0%
! CTTATTTAAGTAAATAGCTC+GGG - chr2_4:59350967-59350986 None:intergenic 25.0%
!! AATGATTGAATGTTGGTTAA+GGG - chr2_4:59351073-59351092 None:intergenic 25.0%
!! AAATGATTGAATGTTGGTTA+AGG - chr2_4:59351074-59351093 None:intergenic 25.0%
! CTAGTGATAATTAATATGTG+TGG - chr2_4:59351202-59351221 None:intergenic 25.0%
! TTATATCTTCGAATCTTTGT+TGG - chr2_4:59351503-59351522 None:intergenic 25.0%
TATGTATGCTTGTATTACAG+GGG + chr2_4:59348442-59348461 Msa0338790:intron 30.0%
AAATGCATGTTATCATGTCA+CGG + chr2_4:59348801-59348820 Msa0338790:intron 30.0%
CATGTATGCATCTTTGTAAA+TGG + chr2_4:59348829-59348848 Msa0338790:intron 30.0%
CATATTGTTTGGTACAATAG+AGG + chr2_4:59348882-59348901 Msa0338790:intron 30.0%
GACCTCAATAAAAATAAACG+TGG + chr2_4:59349160-59349179 Msa0338790:intron 30.0%
!! AGTTTGTGTATTTGGTGTAT+CGG + chr2_4:59349300-59349319 Msa0338790:intron 30.0%
ATAACAGATAGATAGGGAAT+AGG - chr2_4:59349619-59349638 None:intergenic 30.0%
CTAGATGATAACAGATAGAT+AGG - chr2_4:59349626-59349645 None:intergenic 30.0%
GTTTGGAGAATTACATTCTT+TGG - chr2_4:59350075-59350094 None:intergenic 30.0%
CAATCAATTGTGATCTGTAA+TGG + chr2_4:59350280-59350299 Msa0338790:CDS 30.0%
TTGTTGGTCAACAAAATTGT+AGG - chr2_4:59350520-59350539 None:intergenic 30.0%
! CAATTTTGTTGACCAACAAT+AGG + chr2_4:59350521-59350540 Msa0338790:intron 30.0%
AAACATGTAAATAGGCTTTC+AGG - chr2_4:59350666-59350685 None:intergenic 30.0%
CCTTAAAGTGAAATAGACTA+AGG - chr2_4:59350790-59350809 None:intergenic 30.0%
CCTTAGTCTATTTCACTTTA+AGG + chr2_4:59350787-59350806 Msa0338790:intron 30.0%
! ACCTTGCCTTATTAATAAAC+AGG + chr2_4:59351007-59351026 Msa0338790:intron 30.0%
TTGACCTTACATTCATTTGA+TGG + chr2_4:59351395-59351414 Msa0338790:intron 30.0%
TGACCTTACATTCATTTGAT+GGG + chr2_4:59351396-59351415 Msa0338790:intron 30.0%
AATCCCATCAAATGAATGTA+AGG - chr2_4:59351402-59351421 None:intergenic 30.0%
AAAGATTCGAAGATATAACC+AGG + chr2_4:59351505-59351524 Msa0338790:CDS 30.0%
TCTGATGAGAATCTAAACGA+CGG - chr2_4:59348089-59348108 None:intergenic 35.0%
!! TAAGGTAATTAGCTTGCAAG+AGG - chr2_4:59348615-59348634 None:intergenic 35.0%
CAATGTTATTGCAGATGTAC+AGG + chr2_4:59348711-59348730 Msa0338790:intron 35.0%
AAACAAGAGCAGATGATTGA+AGG + chr2_4:59348740-59348759 Msa0338790:CDS 35.0%
! ACTTTTCTCTATCTTGGTCA+CGG - chr2_4:59349018-59349037 None:intergenic 35.0%
!!! GACGTTACTTTTCTCTATCT+TGG - chr2_4:59349024-59349043 None:intergenic 35.0%
!! GTTTGTGTATTTGGTGTATC+GGG + chr2_4:59349301-59349320 Msa0338790:intron 35.0%
AAGTCGCCAAAAATTGTTTG+AGG - chr2_4:59349593-59349612 None:intergenic 35.0%
!! TCTTTTTGCTATGTGACTGA+TGG + chr2_4:59349654-59349673 Msa0338790:intron 35.0%
CACCGTAAATAAAAGAAGAG+AGG - chr2_4:59350116-59350135 None:intergenic 35.0%
!! CTCTCTTCTTTTATTTACGG+TGG + chr2_4:59350114-59350133 Msa0338790:intron 35.0%
ATTATTCCAGGGATGCTTAT+TGG + chr2_4:59350227-59350246 Msa0338790:intron 35.0%
! TATTGGCTAGAGTCTTTCAA+TGG + chr2_4:59350244-59350263 Msa0338790:CDS 35.0%
! ATCCTCTTTGATGGTATTCT+CGG + chr2_4:59350328-59350347 Msa0338790:CDS 35.0%
ATTTACGTACTTGAACAGTC+TGG - chr2_4:59350357-59350376 None:intergenic 35.0%
TAGAAAGGAGACTAATTGAG+AGG - chr2_4:59350384-59350403 None:intergenic 35.0%
GTGATAGAAGAAGTGGAATT+GGG - chr2_4:59350458-59350477 None:intergenic 35.0%
CCTTCGTGAAATAGACTTTA+AGG - chr2_4:59350748-59350767 None:intergenic 35.0%
CCTTAAAGTCTATTTCACGA+AGG + chr2_4:59350745-59350764 Msa0338790:intron 35.0%
AAAGCATACGAAGCTTGATA+TGG - chr2_4:59350930-59350949 None:intergenic 35.0%
GCCTGTTTATTAATAAGGCA+AGG - chr2_4:59351011-59351030 None:intergenic 35.0%
GGCTTGCCTGTTTATTAATA+AGG - chr2_4:59351016-59351035 None:intergenic 35.0%
!! TGAATTGATTTTGTCTTCGG+AGG + chr2_4:59351141-59351160 Msa0338790:intron 35.0%
TAATGTGTGTTGTGTTAGTG+TGG + chr2_4:59351245-59351264 Msa0338790:intron 35.0%
TCTTCTTCTTCTCCTACTAG+CGG + chr2_4:59348015-59348034 Msa0338790:CDS 40.0%
TGAAAAGTTCGCACATCAGA+AGG - chr2_4:59348066-59348085 None:intergenic 40.0%
GGCAACTTTGATCGAACATA+AGG - chr2_4:59348147-59348166 None:intergenic 40.0%
ATGTTCGATCAAAGTTGCCT+CGG + chr2_4:59348148-59348167 Msa0338790:CDS 40.0%
! GTGGCTTTTGACATGAGATA+TGG - chr2_4:59348513-59348532 None:intergenic 40.0%
!! TGAAGAGAAAGAGTACCTGA+AGG - chr2_4:59348535-59348554 None:intergenic 40.0%
TAGGTGTACGTAAGCATAAC+TGG + chr2_4:59348979-59348998 Msa0338790:intron 40.0%
AAGCAAATCCTATCAGCACA+AGG - chr2_4:59349745-59349764 None:intergenic 40.0%
TTTACCAGCTGCAAAGAGAA+AGG + chr2_4:59349976-59349995 Msa0338790:intron 40.0%
CAGATAGTAGCTTGGAACAA+GGG - chr2_4:59350044-59350063 None:intergenic 40.0%
TCAGATAGTAGCTTGGAACA+AGG - chr2_4:59350045-59350064 None:intergenic 40.0%
CCATGATTCAGATAGTAGCT+TGG - chr2_4:59350052-59350071 None:intergenic 40.0%
CCAAGCTACTATCTGAATCA+TGG + chr2_4:59350049-59350068 Msa0338790:CDS 40.0%
GAGTCTTTCAATGGAACTCA+AGG + chr2_4:59350253-59350272 Msa0338790:CDS 40.0%
CACACTTACATCCTCTTTGA+TGG + chr2_4:59350319-59350338 Msa0338790:CDS 40.0%
GGTGATAGAAGAAGTGGAAT+TGG - chr2_4:59350459-59350478 None:intergenic 40.0%
!! TGTGTTGGTGATAGAAGAAG+TGG - chr2_4:59350465-59350484 None:intergenic 40.0%
GACTAAGGTCTTACAGTGAT+AGG - chr2_4:59350775-59350794 None:intergenic 40.0%
! ATTGAATGTTGGTTAAGGGC+GGG - chr2_4:59351069-59351088 None:intergenic 40.0%
! GATTGAATGTTGGTTAAGGG+CGG - chr2_4:59351070-59351089 None:intergenic 40.0%
!! GAATCTTTGTTGGTCGCTTT+TGG - chr2_4:59351493-59351512 None:intergenic 40.0%
TTCGAAGATATAACCAGGAG+AGG + chr2_4:59351510-59351529 Msa0338790:CDS 40.0%
AGAGAGACATCATTTGTGTC+TGG - chr2_4:59351588-59351607 None:intergenic 40.0%
GCACATCAGAAGGTTGTGAA+GGG - chr2_4:59348056-59348075 None:intergenic 45.0%
! GGTGCTTTTAGACTCATCTC+TGG - chr2_4:59348114-59348133 None:intergenic 45.0%
!! GATGAGTCTAAAAGCACCTG+TGG + chr2_4:59348116-59348135 Msa0338790:CDS 45.0%
ACAAAGCAACGGTGAAGATC+TGG - chr2_4:59348186-59348205 None:intergenic 45.0%
GAACTGCATGCACAAAGCAA+CGG - chr2_4:59348197-59348216 None:intergenic 45.0%
GCTTTGTGCATGCAGTTCAA+AGG + chr2_4:59348199-59348218 Msa0338790:CDS 45.0%
TCATGTCAAAAGCCACCTTC+AGG + chr2_4:59348517-59348536 Msa0338790:CDS 45.0%
!! AGAGAAAGAGTACCTGAAGG+TGG - chr2_4:59348532-59348551 None:intergenic 45.0%
CTTGCAAGAGGTGGAAAGAA+TGG - chr2_4:59348603-59348622 None:intergenic 45.0%
!! GGTAATTAGCTTGCAAGAGG+TGG - chr2_4:59348612-59348631 None:intergenic 45.0%
ATGTCCTTTCTCTTTGCAGC+TGG - chr2_4:59349983-59350002 None:intergenic 45.0%
AGCTGCAAAGAGAAAGGACA+TGG + chr2_4:59349982-59350001 Msa0338790:intron 45.0%
CTCCGAGAATACCATCAAAG+AGG - chr2_4:59350333-59350352 None:intergenic 45.0%
! CTCTTTGATGGTATTCTCGG+AGG + chr2_4:59350331-59350350 Msa0338790:CDS 45.0%
AGTCGGATCTGACCTATTGT+TGG - chr2_4:59350536-59350555 None:intergenic 45.0%
AGGTCAGATCCGACTTTGAT+AGG + chr2_4:59350541-59350560 Msa0338790:intron 45.0%
AGGCTCAGACCTATCAAAGT+CGG - chr2_4:59350553-59350572 None:intergenic 45.0%
TAGGTCATACGGGCCTATAA+GGG - chr2_4:59351044-59351063 None:intergenic 45.0%
ATAGGTCATACGGGCCTATA+AGG - chr2_4:59351045-59351064 None:intergenic 45.0%
! TTCATTCCGTTTTGCTCCTC+AGG + chr2_4:59351464-59351483 Msa0338790:intron 45.0%
! TCATTCCGTTTTGCTCCTCA+GGG + chr2_4:59351465-59351484 Msa0338790:intron 45.0%
!! AGAAATTTAAATTAAAAAAT+AGG - chr2_4:59348284-59348303 None:intergenic 5.0%
!!! AAATAAATTTTAATCAATAA+GGG - chr2_4:59351352-59351371 None:intergenic 5.0%
TTCTCCTACTAGCGGAGTTG+TGG + chr2_4:59348023-59348042 Msa0338790:CDS 50.0%
CGCACATCAGAAGGTTGTGA+AGG - chr2_4:59348057-59348076 None:intergenic 50.0%
CGAACATAAGGTCTCACCAC+AGG - chr2_4:59348135-59348154 None:intergenic 50.0%
GTGCATGCAGTTCAAAGGCT+TGG + chr2_4:59348204-59348223 Msa0338790:CDS 50.0%
CATGCAGTTCAAAGGCTTGG+AGG + chr2_4:59348207-59348226 Msa0338790:CDS 50.0%
CCTATCAGCACAAGGTGTAC+CGG - chr2_4:59349737-59349756 None:intergenic 50.0%
CTCTAGCCAATAAGCATCCC+TGG - chr2_4:59350236-59350255 None:intergenic 50.0%
TACGGGCCTATAAGGGTCTA+CGG - chr2_4:59351037-59351056 None:intergenic 50.0%
AAGGGCGGGAATAGGTCATA+CGG - chr2_4:59351055-59351074 None:intergenic 50.0%
! TGTTGGTTAAGGGCGGGAAT+AGG - chr2_4:59351063-59351082 None:intergenic 50.0%
! CATTCCGTTTTGCTCCTCAG+GGG + chr2_4:59351466-59351485 Msa0338790:intron 50.0%
ATTCACTCTCTCTCCTCTCC+TGG - chr2_4:59351526-59351545 None:intergenic 50.0%
!! ACCTCCTGAGCTTGTTGATG+GGG - chr2_4:59351565-59351584 None:intergenic 50.0%
!! GACCTCCTGAGCTTGTTGAT+GGG - chr2_4:59351566-59351585 None:intergenic 50.0%
GTGTCCACAACTCCGCTAGT+AGG - chr2_4:59348030-59348049 None:intergenic 55.0%
CAAAGTTGCCTCGGCTTCGT+TGG + chr2_4:59348157-59348176 Msa0338790:CDS 55.0%
! CCGGTACACCTTGTGCTGAT+AGG + chr2_4:59349734-59349753 Msa0338790:intron 55.0%
GTAGCTTGGAACAAGGGCTG+AGG - chr2_4:59350038-59350057 None:intergenic 55.0%
GGCAAGCCGTAGACCCTTAT+AGG + chr2_4:59351028-59351047 Msa0338790:intron 55.0%
AGGGCGGGAATAGGTCATAC+GGG - chr2_4:59351054-59351073 None:intergenic 55.0%
TGGTCCCCTGAGGAGCAAAA+CGG - chr2_4:59351473-59351492 None:intergenic 55.0%
CTGTCCCCATCAACAAGCTC+AGG + chr2_4:59351558-59351577 Msa0338790:CDS 55.0%
TCCCCATCAACAAGCTCAGG+AGG + chr2_4:59351561-59351580 Msa0338790:CDS 55.0%
!! GGACCTCCTGAGCTTGTTGA+TGG - chr2_4:59351567-59351586 None:intergenic 55.0%
TCTGGTGTCCAACGAAGCCG+AGG - chr2_4:59348168-59348187 None:intergenic 60.0%
GCTTGGAACAAGGGCTGAGG+AGG - chr2_4:59350035-59350054 None:intergenic 60.0%
!!! TGGTCGCTTTTGGTCCCCTG+AGG - chr2_4:59351483-59351502 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 59348003 59351633 59348003 ID=Msa0338790;Name=Msa0338790
chr2_4 mRNA 59348003 59351633 59348003 ID=Msa0338790-mRNA-1;Parent=Msa0338790;Name=Msa0338790-mRNA-1;_AED=0.11;_eAED=0.11;_QI=0|1|0.83|1|1|1|6|7|238
chr2_4 exon 59348003 59348237 59348003 ID=Msa0338790-mRNA-1:exon:18406;Parent=Msa0338790-mRNA-1
chr2_4 exon 59348462 59348538 59348462 ID=Msa0338790-mRNA-1:exon:18407;Parent=Msa0338790-mRNA-1
chr2_4 exon 59348725 59348761 59348725 ID=Msa0338790-mRNA-1:exon:18408;Parent=Msa0338790-mRNA-1
chr2_4 exon 59349984 59350080 59349984 ID=Msa0338790-mRNA-1:exon:18409;Parent=Msa0338790-mRNA-1
chr2_4 exon 59350237 59350366 59350237 ID=Msa0338790-mRNA-1:exon:18410;Parent=Msa0338790-mRNA-1
chr2_4 exon 59351486 59351633 59351486 ID=Msa0338790-mRNA-1:exon:18411;Parent=Msa0338790-mRNA-1
chr2_4 CDS 59348003 59348237 59348003 ID=Msa0338790-mRNA-1:cds;Parent=Msa0338790-mRNA-1
chr2_4 CDS 59348462 59348538 59348462 ID=Msa0338790-mRNA-1:cds;Parent=Msa0338790-mRNA-1
chr2_4 CDS 59348725 59348761 59348725 ID=Msa0338790-mRNA-1:cds;Parent=Msa0338790-mRNA-1
chr2_4 CDS 59349984 59350080 59349984 ID=Msa0338790-mRNA-1:cds;Parent=Msa0338790-mRNA-1
chr2_4 CDS 59350237 59350366 59350237 ID=Msa0338790-mRNA-1:cds;Parent=Msa0338790-mRNA-1
chr2_4 CDS 59351486 59351626 59351486 ID=Msa0338790-mRNA-1:cds;Parent=Msa0338790-mRNA-1
chr2_4 three_prime_UTR 59351627 59351633 59351627 ID=Msa0338790-mRNA-1:three_prime_utr;Parent=Msa0338790-mRNA-1
Gene Sequence

>Msa0338790

ATGTCTTCATCTTCTTCTTCTTCTCCTACTAGCGGAGTTGTGGACACTAACCCTTCACAACCTTCTGATGTGCGAACTTTTCACCGTCGTTTAGATTCTCATCAGACTCCAGAGATGAGTCTAAAAGCACCTGTGGTGAGACCTTATGTTCGATCAAAGTTGCCTCGGCTTCGTTGGACACCAGATCTTCACCGTTGCTTTGTGCATGCAGTTCAAAGGCTTGGAGGAGAAGAGAGGGCTACACCAAAGTTAGTTTTACAATTCATGAATATCAAGGGGCTTACCATATCTCATGTCAAAAGCCACCTTCAGATGTACAGGAGCATGAAACAAGAGCAGATGATTGAAGCTGCAAAGAGAAAGGACATGGCAGCTGATTTATCAGTTTCAGCAATCTCCTCCTCAGCCCTTGTTCCAAGCTACTATCTGAATCATGGCCAAAGAATGGATGCTTATTGGCTAGAGTCTTTCAATGGAACTCAAGGTAATCAATCAATTGTGATCTGTAATGGAGATGAGCAGAAGATGCACACTTACATCCTCTTTGATGGTATTCTCGGAGGCCAGACTGTTCAAGGGACCAAAAGCGACCAACAAAGATTCGAAGATATAACCAGGAGAGGAGAGAGAGTGAATGAAGACACAGTTCTGTCCCCATCAACAAGCTCAGGAGGTCCAGACACAAATGATGTCTCTCTTGAGCTCAAACTCAACTGA
Protein sequence

>Msa0338790

MSSSSSSSPTSGVVDTNPSQPSDVRTFHRRLDSHQTPEMSLKAPVVRPYVRSKLPRLRWTPDLHRCFVHAVQRLGGEERATPKLVLQFMNIKGLTISHVKSHLQMYRSMKQEQMIEAAKRKDMAADLSVSAISSSALVPSYYLNHGQRMDAYWLESFNGTQGNQSIVICNGDEQKMHTYILFDGILGGQTVQGTKSDQQRFEDITRRGERVNEDTVLSPSTSSGGPDTNDVSLELKLN*