AlfalfaGEDB | Msa0339940

M. sativa cultivar zhongmu-4 / Msa0339940

KEGG: K14500
GO: GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009506 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009741 GO:0009742 GO:0009755 GO:0009987 GO:0010033 GO:0014070 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0030054 GO:0032870 GO:0033993 GO:0036211 GO:0038023 GO:0042221 GO:0043170 GO:0043401 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0048545 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0055044 GO:0060089 GO:0065007 GO:0070887 GO:0071310 GO:0071367 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071944 GO:0097305 GO:0140096 GO:1901564 GO:1901700 GO:1901701
PFAM: PF07714.20

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0339940	XP_020224611.1	65.672	335	51	4	19	289	29	363	1.21e-140	417

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0339940	sp\|Q9FIL1\|BSK5_ARATH	56.575	327	78	4	27	289	32	358	6.17e-116	346

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0339940	MtrunA17_Chr4g0032361	60.417	336	68	5	18	289	29	363	3.97e-130	380
Msa0339940	MtrunA17_Chr4g0008911	56.535	329	79	4	25	289	34	362	7.93e-121	353
Msa0339940	MtrunA17_Chr8g0349041	55.319	329	79	5	25	289	34	358	4.32e-116	344
Msa0339940	MtrunA17_Chr8g0386761	53.799	329	84	5	25	289	36	360	2.80e-113	337
Msa0339940	MtrunA17_Chr7g0247441	53.191	329	87	5	25	289	34	359	3.95e-111	331
Msa0339940	MtrunA17_Chr5g0448561	51.090	321	91	6	32	286	69	389	4.78e-100	304
Msa0339940	MtrunA17_Chr4g0054861	47.649	319	103	4	32	286	45	363	6.20e-92	282
Msa0339940	MtrunA17_Chr2g0328731	58.935	263	35	6	68	289	1	231	9.43e-89	266
Msa0339940	MtrunA17_Chr5g0443691	32.422	256	133	8	35	253	87	339	3.12e-26	107
Msa0339940	MtrunA17_Chr5g0416311	32.287	223	106	6	35	215	64	283	5.61e-26	106

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0339940	AT5G59010.1	56.575	327	78	4	27	289	32	358	6.27e-117	346
Msa0339940	AT3G54030.1	54.908	326	82	5	29	289	35	360	8.25e-114	338
Msa0339940	AT5G41260.1	51.488	336	94	6	18	289	28	358	3.89e-110	329
Msa0339940	AT1G63500.1	51.420	352	90	7	15	289	11	358	1.87e-109	327
Msa0339940	AT4G00710.1	52.976	336	88	6	19	289	27	357	1.79e-108	325
Msa0339940	AT4G00710.2	52.976	336	88	6	19	289	27	357	1.79e-108	325
Msa0339940	AT4G35230.1	50.625	320	93	5	32	286	58	377	1.57e-100	305
Msa0339940	AT1G01740.5	50.617	324	89	6	32	289	38	356	1.15e-96	294
Msa0339940	AT1G01740.2	50.617	324	89	6	32	289	38	356	1.53e-96	294
Msa0339940	AT1G01740.1	50.617	324	89	6	32	289	38	356	1.53e-96	294

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Find 70 sgRNAs with CRISPR-Local

Find 489 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACAGTAAATTACACTTTC+TGG	0.153664	2_4:-61215384	None:intergenic
AACAAGAGAGTATACATGGC+TGG	0.485070	2_4:-61215451	None:intergenic
AACCCTAGACTGTCTTGTTT+TGG	0.280656	2_4:+61211876	Msa0339940:CDS
AACCTCATTGCCCATTTCGT+AGG	0.577239	2_4:-61211653	None:intergenic
AATACTGCAGCATTAGAGGA+AGG	0.650812	2_4:+61211708	Msa0339940:CDS
AATGAAACTGTTTCAATTGA+TGG	0.291943	2_4:-61216431	None:intergenic
AATGATTAAAGATCACATGT+TGG	0.596976	2_4:+61211068	Msa0339940:CDS
ACAAGAGAGTATACATGGCT+GGG	0.569082	2_4:-61215450	None:intergenic
ACAGCAACAACCAACCAAGT+TGG	0.612420	2_4:-61211436	None:intergenic
ACAGTTTCATTGACACTGTT+TGG	0.304773	2_4:+61216443	Msa0339940:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATCTTACTATTCACACTATC+TGG	+	chr2_4:61210800-61210819	Msa0339940:intron	30.0%
TCTTACTATTCACACTATCT+GGG	+	chr2_4:61210801-61210820	Msa0339940:intron	30.0%
TTCATTATGGACATAGATGA+TGG	+	chr2_4:61210904-61210923	Msa0339940:intron	30.0%
TCATTATGGACATAGATGAT+GGG	+	chr2_4:61210905-61210924	Msa0339940:intron	30.0%
GAAAACATTGTATCGAAACA+TGG	+	chr2_4:61211015-61211034	Msa0339940:CDS	30.0%
ATTCCTTTGTAGACAACATT+GGG	-	chr2_4:61211051-61211070	None:intergenic	30.0%
TTCTGTAAGCATTAAGATCA+TGG	-	chr2_4:61211742-61211761	None:intergenic	30.0%
AGAAAAGATGGTAAAAGCTA+CGG	+	chr2_4:61211912-61211931	Msa0339940:CDS	30.0%
TCTACACACAAAAAAATGCA+TGG	-	chr2_4:61212053-61212072	None:intergenic	30.0%
TACGAATAACACATGATTCA+AGG	-	chr2_4:61212593-61212612	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_4	gene	61210536	61217157	61210536	ID=Msa0339940;Name=Msa0339940
chr2_4	mRNA	61210536	61217157	61210536	ID=Msa0339940-mRNA-1;Parent=Msa0339940;Name=Msa0339940-mRNA-1;_AED=0.15;_eAED=0.25;_QI=0\|0\|0\|0.9\|0.11\|0\|10\|0\|319
chr2_4	exon	61210536	61210541	61210536	ID=Msa0339940-mRNA-1:exon:19162;Parent=Msa0339940-mRNA-1
chr2_4	exon	61210642	61210687	61210642	ID=Msa0339940-mRNA-1:exon:19163;Parent=Msa0339940-mRNA-1
chr2_4	exon	61210920	61211143	61210920	ID=Msa0339940-mRNA-1:exon:19164;Parent=Msa0339940-mRNA-1
chr2_4	exon	61211423	61211528	61211423	ID=Msa0339940-mRNA-1:exon:19165;Parent=Msa0339940-mRNA-1
chr2_4	exon	61211642	61211765	61211642	ID=Msa0339940-mRNA-1:exon:19166;Parent=Msa0339940-mRNA-1
chr2_4	exon	61211863	61211933	61211863	ID=Msa0339940-mRNA-1:exon:19167;Parent=Msa0339940-mRNA-1
chr2_4	exon	61215373	61215458	61215373	ID=Msa0339940-mRNA-1:exon:19168;Parent=Msa0339940-mRNA-1
chr2_4	exon	61215916	61215987	61215916	ID=Msa0339940-mRNA-1:exon:19169;Parent=Msa0339940-mRNA-1
chr2_4	exon	61216389	61216546	61216389	ID=Msa0339940-mRNA-1:exon:19170;Parent=Msa0339940-mRNA-1
chr2_4	exon	61217091	61217157	61217091	ID=Msa0339940-mRNA-1:exon:19171;Parent=Msa0339940-mRNA-1
chr2_4	CDS	61210536	61210541	61210536	ID=Msa0339940-mRNA-1:cds;Parent=Msa0339940-mRNA-1
chr2_4	CDS	61210642	61210687	61210642	ID=Msa0339940-mRNA-1:cds;Parent=Msa0339940-mRNA-1
chr2_4	CDS	61210920	61211143	61210920	ID=Msa0339940-mRNA-1:cds;Parent=Msa0339940-mRNA-1
chr2_4	CDS	61211423	61211528	61211423	ID=Msa0339940-mRNA-1:cds;Parent=Msa0339940-mRNA-1
chr2_4	CDS	61211642	61211765	61211642	ID=Msa0339940-mRNA-1:cds;Parent=Msa0339940-mRNA-1
chr2_4	CDS	61211863	61211933	61211863	ID=Msa0339940-mRNA-1:cds;Parent=Msa0339940-mRNA-1
chr2_4	CDS	61215373	61215458	61215373	ID=Msa0339940-mRNA-1:cds;Parent=Msa0339940-mRNA-1
chr2_4	CDS	61215916	61215987	61215916	ID=Msa0339940-mRNA-1:cds;Parent=Msa0339940-mRNA-1
chr2_4	CDS	61216389	61216546	61216389	ID=Msa0339940-mRNA-1:cds;Parent=Msa0339940-mRNA-1
chr2_4	CDS	61217091	61217157	61217091	ID=Msa0339940-mRNA-1:cds;Parent=Msa0339940-mRNA-1

Gene Sequence

>Msa0339940

ATGGTGAGGATACAAACAACAAGAGCAAGAAGAAGACAGAAGTGGTGCTGTAATGATGGGAACAAGGGTGAGGAGAAGGATTCTTGGGGTGGTTTCACTAAGTTTAGCTTGGACCAACTAAGAACTGCCACGCCAGGATTTTCACCCGAAAACATTGTATCGAAACATGGTGAGAAGGCTCCCAATGTTGTCTACAAAGGAATGATTAAAGATCACATGTTGGTTGCTGTGAAGCGTTTCAACAAGTCTGCTTGGCCTGATTCACACCAATTCCTGAATGATAGGTTAGCCAACTTGGTTGGTTGTTGCTGTGAAGGAGAAGAGAGATTGCTAGTTGCAGAATTTACGCCTAATGAAACTCTCTCTAAGCATCTCTTTCGCTGGGAGACTCAGCCTACGAAATGGGCAATGAGGTTGAGACTGGCCTTGTATTTAGCACAAGCTTTGGAATACTGCAGCATTAGAGGAAGGCCATTGTACCATGATCTTAATGCTTACAGAATTTTGACGGCTGATGGCAACCCTAGACTGTCTTGTTTTGGACTAATGAAAAACAGAAAAGATGGTAAAAGCTACGGAAATATTACTCCAGAAAGTGTAATTTACTGTTTTGGAACACCTTTGCTTGATCTTCAGAGTGGAAAGCATATCCCACCCAGCCATTACGAACCTCGTGAAAGACCAAATGCCAAGTCATCTGTGAATGCTCTAACTCATCTTCAAAAAGAAACTTCAGTTTCATCACTTGTTTTATTGGGTATACTAGATGAGAGTGAACCATCAATTGAAACAGTTTCATTGACACTGTTTGGGGAAGCTTGTTCTAGAAAAGATCTCATTACAATACACAAAATTTTGGAAAGAGTGGTATTGATGAAGATGTTGCAAATGAGATCAAATACACCTGGCCTACTTCTATCTTCAAGCAGCAGCCTTCTTCAACCTTCGGATGGACATTGA

Protein sequence

>Msa0339940

MVRIQTTRARRRQKWCCNDGNKGEEKDSWGGFTKFSLDQLRTATPGFSPENIVSKHGEKAPNVVYKGMIKDHMLVAVKRFNKSAWPDSHQFLNDRLANLVGCCCEGEERLLVAEFTPNETLSKHLFRWETQPTKWAMRLRLALYLAQALEYCSIRGRPLYHDLNAYRILTADGNPRLSCFGLMKNRKDGKSYGNITPESVIYCFGTPLLDLQSGKHIPPSHYEPRERPNAKSSVNALTHLQKETSVSSLVLLGILDESEPSIETVSLTLFGEACSRKDLITIHKILERVVLMKMLQMRSNTPGLLLSSSSSLLQPSDGH*