AlfalfaGEDB | Msa0344420

M. sativa cultivar zhongmu-4 / Msa0344420

KEGG: K08959|K08960
GO: GO:0000902 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006897 GO:0006950 GO:0007275 GO:0008150 GO:0008152 GO:0008360 GO:0009266 GO:0009409 GO:0009628 GO:0009653 GO:0009719 GO:0009725 GO:0009741 GO:0009826 GO:0009850 GO:0009987 GO:0010033 GO:0010817 GO:0014070 GO:0016043 GO:0016049 GO:0016192 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0022603 GO:0022604 GO:0022622 GO:0032501 GO:0032502 GO:0032989 GO:0033993 GO:0036211 GO:0040007 GO:0042221 GO:0042445 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0046777 GO:0048364 GO:0048589 GO:0048731 GO:0048856 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051128 GO:0051179 GO:0051234 GO:0060560 GO:0065007 GO:0065008 GO:0071704 GO:0071840 GO:0098657 GO:0099402 GO:0140096 GO:1901564 GO:1901700
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0344420	KEH15799.1	83.827	371	13	2	1	324	1	371	0.0	619

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0344420	sp\|Q9LW62\|CKL10_ARATH	72.171	327	58	3	1	296	1	325	2.12e-167	476

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0344420	A0A072TET6	83.827	371	13	2	1	324	1	371	0.0	619

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0344420	MtrunA17_Chr1g0167261	82.486	354	25	2	1	324	2	348	0.0	580
Msa0344420	MtrunA17_Chr1g0166821	70.270	370	61	4	1	324	2	368	0.0	511
Msa0344420	MtrunA17_Chr1g0187571	67.030	367	68	4	1	316	2	366	2.50e-172	486
Msa0344420	MtrunA17_Chr7g0261501	70.031	327	64	4	1	295	1	325	1.62e-160	456
Msa0344420	MtrunA17_Chr1g0183631	71.148	305	56	2	1	275	1	303	1.55e-156	446
Msa0344420	MtrunA17_Chr4g0071031	60.843	332	94	3	1	298	1	330	7.54e-144	413
Msa0344420	MtrunA17_Chr2g0329981	59.429	350	103	4	1	316	1	345	8.54e-144	414
Msa0344420	MtrunA17_Chr8g0342821	62.579	318	84	4	1	288	1	313	3.73e-140	405
Msa0344420	MtrunA17_Chr6g0486611	61.981	313	86	3	1	283	1	310	1.99e-137	396
Msa0344420	MtrunA17_Chr8g0390211	56.022	357	108	5	1	313	1	352	3.59e-136	393

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0344420	AT3G23340.1	72.171	327	58	3	1	296	1	325	2.15e-168	476
Msa0344420	AT4G14340.1	64.151	371	78	6	1	318	7	375	9.48e-162	459
Msa0344420	AT4G14340.2	68.693	329	70	3	1	298	7	333	1.31e-160	453
Msa0344420	AT1G03930.1	66.567	335	74	3	1	299	1	333	1.65e-157	449
Msa0344420	AT4G28540.1	62.921	356	90	4	1	316	5	358	7.57e-157	447
Msa0344420	AT3G23340.2	69.113	327	54	4	1	296	1	311	4.26e-156	444
Msa0344420	AT5G43320.1	66.472	343	77	5	1	307	1	341	5.77e-155	443
Msa0344420	AT5G44100.1	66.567	335	73	4	1	299	1	332	6.08e-155	442
Msa0344420	AT5G44100.2	66.567	335	73	4	1	299	1	332	6.08e-155	442
Msa0344420	AT5G43320.2	67.262	336	73	4	1	301	1	334	4.50e-154	436

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Find 69 sgRNAs with CRISPR-Local

Find 210 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTTGGGAGTGGAGCATT+TGG	0.356250	3_1:-2911504	Msa0344420:CDS
AAAGAAGCTAACTCCAATAG+AGG	0.515041	3_1:-2908808	Msa0344420:intron
AAAGCCCGAGAACTTTCTAA+TGG	0.388867	3_1:-2909947	Msa0344420:CDS
AAATATGACGTGAACTCTGT+TGG	0.622690	3_1:+2908586	None:intergenic
AAATTGAAGGATCCACAAAG+TGG	0.696056	3_1:-2908350	Msa0344420:CDS
AACTTGGGAGTGGAGCATTT+GGG	0.378231	3_1:-2911503	Msa0344420:CDS
AAGCCCGAGAACTTTCTAAT+GGG	0.468302	3_1:-2909946	Msa0344420:CDS
AATCATGGAAGAACATCTGA+AGG	0.597406	3_1:+2907828	None:intergenic
ACATCTGAAGGTGCATACGA+CGG	0.698148	3_1:+2907840	None:intergenic
ACTGCAATGATCGACAATAG+TGG	0.469224	3_1:+2908563	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGTATAGGTATGATGTGTAT+GGG	+	chr3_1:2908558-2908577	None:intergenic	30.0%
ACTCAACATATTTACTTGTC+GGG	-	chr3_1:2908603-2908622	Msa0344420:CDS	30.0%
AAATTGTATAGGCTTCTTCA+AGG	-	chr3_1:2908891-2908910	Msa0344420:intron	30.0%
CCGCTTATTAACAAAATTGT+CGG	+	chr3_1:2908963-2908982	None:intergenic	30.0%
ATACCTCATATAAAGTGGTA+TGG	-	chr3_1:2909132-2909151	Msa0344420:intron	30.0%
AGCTATACTTACTAGTTGAT+CGG	+	chr3_1:2909268-2909287	None:intergenic	30.0%
TTTGCTTCATGCTAGAAATA+TGG	+	chr3_1:2909313-2909332	None:intergenic	30.0%
TCTAGTTAAGATAGTACACA+TGG	+	chr3_1:2909357-2909376	None:intergenic	30.0%
AATGTATAATGACGAGTACA+AGG	+	chr3_1:2910448-2910467	None:intergenic	30.0%
CTAAATATTGGTACCTCTAT+TGG	+	chr3_1:2910581-2910600	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_1	gene	2907830	2911565	2907830	ID=Msa0344420;Name=Msa0344420
chr3_1	mRNA	2907830	2911565	2907830	ID=Msa0344420-mRNA-1;Parent=Msa0344420;Name=Msa0344420-mRNA-1;_AED=0.19;_eAED=0.19;_QI=0\|0\|0\|1\|0.90\|0.91\|12\|0\|324
chr3_1	exon	2911490	2911565	2911490	ID=Msa0344420-mRNA-1:exon:953;Parent=Msa0344420-mRNA-1
chr3_1	exon	2911369	2911409	2911369	ID=Msa0344420-mRNA-1:exon:952;Parent=Msa0344420-mRNA-1
chr3_1	exon	2910120	2910248	2910120	ID=Msa0344420-mRNA-1:exon:951;Parent=Msa0344420-mRNA-1
chr3_1	exon	2909924	2910019	2909924	ID=Msa0344420-mRNA-1:exon:950;Parent=Msa0344420-mRNA-1
chr3_1	exon	2909489	2909559	2909489	ID=Msa0344420-mRNA-1:exon:949;Parent=Msa0344420-mRNA-1
chr3_1	exon	2909166	2909227	2909166	ID=Msa0344420-mRNA-1:exon:948;Parent=Msa0344420-mRNA-1
chr3_1	exon	2908981	2909044	2908981	ID=Msa0344420-mRNA-1:exon:947;Parent=Msa0344420-mRNA-1
chr3_1	exon	2908809	2908899	2908809	ID=Msa0344420-mRNA-1:exon:946;Parent=Msa0344420-mRNA-1
chr3_1	exon	2908500	2908626	2908500	ID=Msa0344420-mRNA-1:exon:945;Parent=Msa0344420-mRNA-1
chr3_1	exon	2908331	2908404	2908331	ID=Msa0344420-mRNA-1:exon:944;Parent=Msa0344420-mRNA-1
chr3_1	exon	2908185	2908245	2908185	ID=Msa0344420-mRNA-1:exon:943;Parent=Msa0344420-mRNA-1
chr3_1	exon	2907830	2907912	2907830	ID=Msa0344420-mRNA-1:exon:942;Parent=Msa0344420-mRNA-1
chr3_1	CDS	2911490	2911565	2911490	ID=Msa0344420-mRNA-1:cds;Parent=Msa0344420-mRNA-1
chr3_1	CDS	2911369	2911409	2911369	ID=Msa0344420-mRNA-1:cds;Parent=Msa0344420-mRNA-1
chr3_1	CDS	2910120	2910248	2910120	ID=Msa0344420-mRNA-1:cds;Parent=Msa0344420-mRNA-1
chr3_1	CDS	2909924	2910019	2909924	ID=Msa0344420-mRNA-1:cds;Parent=Msa0344420-mRNA-1
chr3_1	CDS	2909489	2909559	2909489	ID=Msa0344420-mRNA-1:cds;Parent=Msa0344420-mRNA-1
chr3_1	CDS	2909166	2909227	2909166	ID=Msa0344420-mRNA-1:cds;Parent=Msa0344420-mRNA-1
chr3_1	CDS	2908981	2909044	2908981	ID=Msa0344420-mRNA-1:cds;Parent=Msa0344420-mRNA-1
chr3_1	CDS	2908809	2908899	2908809	ID=Msa0344420-mRNA-1:cds;Parent=Msa0344420-mRNA-1
chr3_1	CDS	2908500	2908626	2908500	ID=Msa0344420-mRNA-1:cds;Parent=Msa0344420-mRNA-1
chr3_1	CDS	2908331	2908404	2908331	ID=Msa0344420-mRNA-1:cds;Parent=Msa0344420-mRNA-1
chr3_1	CDS	2908185	2908245	2908185	ID=Msa0344420-mRNA-1:cds;Parent=Msa0344420-mRNA-1
chr3_1	CDS	2907830	2907912	2907830	ID=Msa0344420-mRNA-1:cds;Parent=Msa0344420-mRNA-1

Gene Sequence

>Msa0344420

ATGGATATTGTTATTGGTGGAAAATTCAAGCTTGGCAGCAAACTTGGGAGTGGAGCATTTGGGGAGCTTTATTTGGGTGTTGATGTGAAAACTCAAGAGGAAGTGGCTCTTAAACTGTGGTATGGAGTCGAGGGTGACTACAATGTGATGGCTGTTGACCTTCTTGGACCAAGTCTTGAAGACTTATTTAATTACTGCAATAGAAAATTCTCCTTAAAAACAGTATTGATGCTTGCCGATCAACTAATAAATAGAGTAGCGTATATGCACTATCGTGGATTTCTGCATCGTGATGTAAAGCCCGAGAACTTTCTAATGGGTTCAGGCAGCAAGATAAATCAGGTGTACATTATTGACTATGGCCTTGCAAAAAAGTATTGTGATCTTAAAACACAAAGGCACATACCATACAGGGAAAACAAGAATCTCATTGGAACTGCTCAATATGCAAGTTTTAACACTCACCTTGGAATTGAACAAAGTAGAAGAGATGATCTAGAATCGCTTGGTTATGTACTAATGTACTTTTTGAGAGGAAGTCTTCCATGGCATGGGCACGGCATGAAAGCTGTCACTAAAGAGAAGAAATATGAATGGATAAGGGAAAAAAAGAAGCTAACTCCAATAGAGGTCTTGTGCAAGTCACATCCAACAGAGTTCACGTCATATTTCCACTATTGTCGATCATTGCAGTTTGAAGATAAACCTGATTATTCATATCTTAAGAAACTCTTTCGGGAACTATTCATTAGAGAAGGTTATCAGTTTGACTTTGTATTTGATTGGACTAAATTGAAGGATCCACAAAGTGGTTCCAGTTCTAAAACAAGTCCAAATGTGAAACCAGTCTTAAACACAGGACCATATGAAGAGAGATTAGAACGGCCTACAGGAAGAAATAATGGCTCTGGGGACAGACTGCAGCATAGGTCTTTGGCAGATCCGTCGTATGCACCTTCAGATGTTCTTCCATGA

Protein sequence

>Msa0344420

MDIVIGGKFKLGSKLGSGAFGELYLGVDVKTQEEVALKLWYGVEGDYNVMAVDLLGPSLEDLFNYCNRKFSLKTVLMLADQLINRVAYMHYRGFLHRDVKPENFLMGSGSKINQVYIIDYGLAKKYCDLKTQRHIPYRENKNLIGTAQYASFNTHLGIEQSRRDDLESLGYVLMYFLRGSLPWHGHGMKAVTKEKKYEWIREKKKLTPIEVLCKSHPTEFTSYFHYCRSLQFEDKPDYSYLKKLFRELFIREGYQFDFVFDWTKLKDPQSGSSSKTSPNVKPVLNTGPYEERLERPTGRNNGSGDRLQHRSLADPSYAPSDVLP*