AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0345240


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0345240 MtrunA17_Chr3g0090311 56.284 183 51 5 4 158 2 183 1.34e-57 177
Msa0345240 MtrunA17_Chr3g0098461 36.646 161 95 4 4 159 6 164 1.71e-18 77.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0345240 AT5G13780.1 53.548 155 59 4 4 146 2 155 2.93e-47 152

Find 39 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACCTGTTCCATGTCGTTT+TGG 0.275526 3_1:+4389967 None:intergenic
AAGGGGAAGCAGATGCATTC+TGG 0.323040 3_1:-4389079 Msa0345240:CDS
TCTAAGATGTACAAACTCTT+TGG 0.380946 3_1:-4390176 Msa0345240:CDS
ACATTGGGATACAAGATTCA+TGG 0.416619 3_1:-4389163 Msa0345240:CDS
AGGGGAAGCAGATGCATTCT+GGG 0.418741 3_1:-4389078 Msa0345240:CDS
GGCCGTGAGAAGAGGGCAAC+AGG 0.424430 3_1:+4390146 None:intergenic
CTCCCAAAACGACATGGAAC+AGG 0.433249 3_1:-4389970 Msa0345240:intron
TGCATTCTGGGGAGACTAAA+GGG 0.439115 3_1:-4389066 Msa0345240:CDS
TTGTATTTAACACAGATGTT+TGG 0.469950 3_1:-4389250 Msa0345240:intron
GGGATACAAGATTCATGGAT+TGG 0.477746 3_1:-4389158 Msa0345240:CDS
CACGTCGTAGAAGATATTAA+TGG 0.486544 3_1:-4390113 Msa0345240:CDS
TAATTTGTTCAAAGAGACAT+TGG 0.499278 3_1:-4389179 Msa0345240:CDS
TCTCTGATGGGTTGGGAGAA+CGG 0.508987 3_1:+4390022 None:intergenic
ATGCATTCTGGGGAGACTAA+AGG 0.509032 3_1:-4389067 Msa0345240:CDS
TACCACGTCTCTGATGGGTT+GGG 0.526888 3_1:+4390015 None:intergenic
AATTTGTTCAAAGAGACATT+GGG 0.527731 3_1:-4389178 Msa0345240:CDS
TAGCGATACCACGTCTCTGA+TGG 0.539259 3_1:+4390009 None:intergenic
ATACCACGTCTCTGATGGGT+TGG 0.540598 3_1:+4390014 None:intergenic
GAGTTGAGGCCGTGAGAAGA+GGG 0.546949 3_1:+4390139 None:intergenic
TGAACAAATTAAAAGCTGCA+TGG 0.554368 3_1:+4389191 None:intergenic
ATGGGTTGGGAGAACGGCGA+GGG 0.570608 3_1:+4390028 None:intergenic
GAAATGAGAAAGCAACTCAA+GGG 0.572712 3_1:-4389097 Msa0345240:CDS
TGAGTTGAGGCCGTGAGAAG+AGG 0.574852 3_1:+4390138 None:intergenic
TGAAATGAGAAAGCAACTCA+AGG 0.580702 3_1:-4389098 Msa0345240:CDS
AGCGATACCACGTCTCTGAT+GGG 0.588160 3_1:+4390010 None:intergenic
GATTGCCTCCCAAAACGACA+TGG 0.596635 3_1:-4389976 Msa0345240:CDS
ATTCAAGAGGAAATTAGTGA+AGG 0.600654 3_1:-4390062 Msa0345240:CDS
AGCCTGTTGCCCTCTTCTCA+CGG 0.606181 3_1:-4390148 Msa0345240:CDS
GATGGGTTGGGAGAACGGCG+AGG 0.612867 3_1:+4390027 None:intergenic
CGTCCACTGTGAAATTCAAG+AGG 0.617845 3_1:-4390075 Msa0345240:CDS
ATACAAGATTCATGGATTGG+AGG 0.623983 3_1:-4389155 Msa0345240:CDS
TTGAATTTCACAGTGGACGT+AGG 0.625067 3_1:+4390079 None:intergenic
AGAGTTTGTACATCTTAGAG+AGG 0.637261 3_1:+4390180 None:intergenic
CTCCCAACCCATCAGAGACG+TGG 0.645269 3_1:-4390017 Msa0345240:CDS
AAATGAGAAAGCAACTCAAG+GGG 0.646235 3_1:-4389096 Msa0345240:CDS
GGGGAAGCAGATGCATTCTG+GGG 0.660086 3_1:-4389077 Msa0345240:CDS
ACATCTTAGAGAGGTCGCAA+AGG 0.660670 3_1:+4390189 None:intergenic
TCTACGACGTGAATGAGTTG+AGG 0.674386 3_1:+4390125 None:intergenic
TTTCCTCTTGAATTTCACAG+TGG 0.694893 3_1:+4390072 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATATGTATCTTTTATATT+TGG - chr3_1:4389722-4389741 Msa0345240:intron 10.0%
!!! TAATATGTATCTTTTATATT+TGG - chr3_1:4389722-4389741 Msa0345240:intron 10.0%
!! AAAATGATCCTTAATTATTT+CGG + chr3_1:4389673-4389692 None:intergenic 15.0%
!! TTCAAGAAAATTATTTGAAT+AGG + chr3_1:4389871-4389890 None:intergenic 15.0%
!! AAAATGATCCTTAATTATTT+CGG + chr3_1:4389673-4389692 None:intergenic 15.0%
!! TTCAAGAAAATTATTTGAAT+AGG + chr3_1:4389871-4389890 None:intergenic 15.0%
!!! ATTTTTAGCCGAAATAATTA+AGG - chr3_1:4389662-4389681 Msa0345240:intron 20.0%
!!! ATTTTTAGCCGAAATAATTA+AGG - chr3_1:4389662-4389681 Msa0345240:intron 20.0%
! ATCAAATTAATCAATGCATC+TGG - chr3_1:4389587-4389606 Msa0345240:intron 25.0%
!!! ACTTGTTACAGTTTTGTTAT+TGG - chr3_1:4389614-4389633 Msa0345240:intron 25.0%
! TTGTATTTAACACAGATGTT+TGG - chr3_1:4390016-4390035 Msa0345240:CDS 25.0%
!!! TAATTTGTTCAAAGAGACAT+TGG - chr3_1:4390087-4390106 Msa0345240:CDS 25.0%
!! AATTTGTTCAAAGAGACATT+GGG - chr3_1:4390088-4390107 Msa0345240:CDS 25.0%
! ATCAAATTAATCAATGCATC+TGG - chr3_1:4389587-4389606 Msa0345240:intron 25.0%
!!! ACTTGTTACAGTTTTGTTAT+TGG - chr3_1:4389614-4389633 Msa0345240:intron 25.0%
! TTGTATTTAACACAGATGTT+TGG - chr3_1:4390016-4390035 Msa0345240:CDS 25.0%
!!! TAATTTGTTCAAAGAGACAT+TGG - chr3_1:4390087-4390106 Msa0345240:CDS 25.0%
!! AATTTGTTCAAAGAGACATT+GGG - chr3_1:4390088-4390107 Msa0345240:CDS 25.0%
! TCTAAGATGTACAAACTCTT+TGG - chr3_1:4389090-4389109 Msa0345240:CDS 30.0%
ATTCAAGAGGAAATTAGTGA+AGG - chr3_1:4389204-4389223 Msa0345240:CDS 30.0%
TATTAATTCAAATTGCACCG+AGG - chr3_1:4389371-4389390 Msa0345240:intron 30.0%
ATTAATTCAAATTGCACCGA+GGG - chr3_1:4389372-4389391 Msa0345240:intron 30.0%
GAGTCAAGTTATTAACTACT+TGG - chr3_1:4389756-4389775 Msa0345240:intron 30.0%
ATATGTAGAATTGCAGAACT+AGG + chr3_1:4389909-4389928 None:intergenic 30.0%
TGTTATGAGTTAGATTCATC+AGG - chr3_1:4389928-4389947 Msa0345240:intron 30.0%
AAGTGTGTCTTCTTGTAATA+GGG - chr3_1:4389962-4389981 Msa0345240:intron 30.0%
TGAACAAATTAAAAGCTGCA+TGG + chr3_1:4390078-4390097 None:intergenic 30.0%
!! CAAAGTTTTATGCTGATGAT+GGG - chr3_1:4390134-4390153 Msa0345240:CDS 30.0%
!! AAAGTTTTATGCTGATGATG+GGG - chr3_1:4390135-4390154 Msa0345240:CDS 30.0%
! TCTAAGATGTACAAACTCTT+TGG - chr3_1:4389090-4389109 Msa0345240:CDS 30.0%
ATTCAAGAGGAAATTAGTGA+AGG - chr3_1:4389204-4389223 Msa0345240:CDS 30.0%
TATTAATTCAAATTGCACCG+AGG - chr3_1:4389371-4389390 Msa0345240:intron 30.0%
ATTAATTCAAATTGCACCGA+GGG - chr3_1:4389372-4389391 Msa0345240:intron 30.0%
GAGTCAAGTTATTAACTACT+TGG - chr3_1:4389756-4389775 Msa0345240:intron 30.0%
ATATGTAGAATTGCAGAACT+AGG + chr3_1:4389909-4389928 None:intergenic 30.0%
TGTTATGAGTTAGATTCATC+AGG - chr3_1:4389928-4389947 Msa0345240:intron 30.0%
AAGTGTGTCTTCTTGTAATA+GGG - chr3_1:4389962-4389981 Msa0345240:intron 30.0%
TGAACAAATTAAAAGCTGCA+TGG + chr3_1:4390078-4390097 None:intergenic 30.0%
!! CAAAGTTTTATGCTGATGAT+GGG - chr3_1:4390134-4390153 Msa0345240:CDS 30.0%
!! AAAGTTTTATGCTGATGATG+GGG - chr3_1:4390135-4390154 Msa0345240:CDS 30.0%
AGAGTTTGTACATCTTAGAG+AGG + chr3_1:4389089-4389108 None:intergenic 35.0%
CACGTCGTAGAAGATATTAA+TGG - chr3_1:4389153-4389172 Msa0345240:CDS 35.0%
TTTCCTCTTGAATTTCACAG+TGG + chr3_1:4389197-4389216 None:intergenic 35.0%
GAAGTGTGTCTTCTTGTAAT+AGG - chr3_1:4389961-4389980 Msa0345240:intron 35.0%
ACATTGGGATACAAGATTCA+TGG - chr3_1:4390103-4390122 Msa0345240:CDS 35.0%
!! ATACAAGATTCATGGATTGG+AGG - chr3_1:4390111-4390130 Msa0345240:CDS 35.0%
!! GCAAAGTTTTATGCTGATGA+TGG - chr3_1:4390133-4390152 Msa0345240:CDS 35.0%
TGAAATGAGAAAGCAACTCA+AGG - chr3_1:4390168-4390187 Msa0345240:CDS 35.0%
GAAATGAGAAAGCAACTCAA+GGG - chr3_1:4390169-4390188 Msa0345240:CDS 35.0%
AAATGAGAAAGCAACTCAAG+GGG - chr3_1:4390170-4390189 Msa0345240:CDS 35.0%
AGAGTTTGTACATCTTAGAG+AGG + chr3_1:4389089-4389108 None:intergenic 35.0%
CACGTCGTAGAAGATATTAA+TGG - chr3_1:4389153-4389172 Msa0345240:CDS 35.0%
TTTCCTCTTGAATTTCACAG+TGG + chr3_1:4389197-4389216 None:intergenic 35.0%
GAAGTGTGTCTTCTTGTAAT+AGG - chr3_1:4389961-4389980 Msa0345240:intron 35.0%
ACATTGGGATACAAGATTCA+TGG - chr3_1:4390103-4390122 Msa0345240:CDS 35.0%
!! ATACAAGATTCATGGATTGG+AGG - chr3_1:4390111-4390130 Msa0345240:CDS 35.0%
!! GCAAAGTTTTATGCTGATGA+TGG - chr3_1:4390133-4390152 Msa0345240:CDS 35.0%
TGAAATGAGAAAGCAACTCA+AGG - chr3_1:4390168-4390187 Msa0345240:CDS 35.0%
GAAATGAGAAAGCAACTCAA+GGG - chr3_1:4390169-4390188 Msa0345240:CDS 35.0%
AAATGAGAAAGCAACTCAAG+GGG - chr3_1:4390170-4390189 Msa0345240:CDS 35.0%
TTGAATTTCACAGTGGACGT+AGG + chr3_1:4389190-4389209 None:intergenic 40.0%
!!! AACCTGTTCCATGTCGTTTT+GGG + chr3_1:4389301-4389320 None:intergenic 40.0%
!! AAACCTGTTCCATGTCGTTT+TGG + chr3_1:4389302-4389321 None:intergenic 40.0%
GAAACTGAGTTTGAATCCCT+CGG + chr3_1:4389391-4389410 None:intergenic 40.0%
! CTCAGTTTCTGAGAGTTACA+AGG - chr3_1:4389402-4389421 Msa0345240:intron 40.0%
GGGATACAAGATTCATGGAT+TGG - chr3_1:4390108-4390127 Msa0345240:CDS 40.0%
TTGAATTTCACAGTGGACGT+AGG + chr3_1:4389190-4389209 None:intergenic 40.0%
!!! AACCTGTTCCATGTCGTTTT+GGG + chr3_1:4389301-4389320 None:intergenic 40.0%
!! AAACCTGTTCCATGTCGTTT+TGG + chr3_1:4389302-4389321 None:intergenic 40.0%
GAAACTGAGTTTGAATCCCT+CGG + chr3_1:4389391-4389410 None:intergenic 40.0%
! CTCAGTTTCTGAGAGTTACA+AGG - chr3_1:4389402-4389421 Msa0345240:intron 40.0%
GGGATACAAGATTCATGGAT+TGG - chr3_1:4390108-4390127 Msa0345240:CDS 40.0%
ACATCTTAGAGAGGTCGCAA+AGG + chr3_1:4389080-4389099 None:intergenic 45.0%
TCTACGACGTGAATGAGTTG+AGG + chr3_1:4389144-4389163 None:intergenic 45.0%
CGTCCACTGTGAAATTCAAG+AGG - chr3_1:4389191-4389210 Msa0345240:CDS 45.0%
!!! GTTTTATGCTGATGATGGGG+AGG - chr3_1:4390138-4390157 Msa0345240:CDS 45.0%
! ATGCATTCTGGGGAGACTAA+AGG - chr3_1:4390199-4390218 Msa0345240:CDS 45.0%
! TGCATTCTGGGGAGACTAAA+GGG - chr3_1:4390200-4390219 Msa0345240:CDS 45.0%
ACATCTTAGAGAGGTCGCAA+AGG + chr3_1:4389080-4389099 None:intergenic 45.0%
TCTACGACGTGAATGAGTTG+AGG + chr3_1:4389144-4389163 None:intergenic 45.0%
CGTCCACTGTGAAATTCAAG+AGG - chr3_1:4389191-4389210 Msa0345240:CDS 45.0%
!!! GTTTTATGCTGATGATGGGG+AGG - chr3_1:4390138-4390157 Msa0345240:CDS 45.0%
! ATGCATTCTGGGGAGACTAA+AGG - chr3_1:4390199-4390218 Msa0345240:CDS 45.0%
! TGCATTCTGGGGAGACTAAA+GGG - chr3_1:4390200-4390219 Msa0345240:CDS 45.0%
!! TCTCTGATGGGTTGGGAGAA+CGG + chr3_1:4389247-4389266 None:intergenic 50.0%
!! TACCACGTCTCTGATGGGTT+GGG + chr3_1:4389254-4389273 None:intergenic 50.0%
! ATACCACGTCTCTGATGGGT+TGG + chr3_1:4389255-4389274 None:intergenic 50.0%
AGCGATACCACGTCTCTGAT+GGG + chr3_1:4389259-4389278 None:intergenic 50.0%
TAGCGATACCACGTCTCTGA+TGG + chr3_1:4389260-4389279 None:intergenic 50.0%
GATTGCCTCCCAAAACGACA+TGG - chr3_1:4389290-4389309 Msa0345240:intron 50.0%
!!! CTGTTCCATGTCGTTTTGGG+AGG + chr3_1:4389298-4389317 None:intergenic 50.0%
CTCCCAAAACGACATGGAAC+AGG - chr3_1:4389296-4389315 Msa0345240:intron 50.0%
AAGGGGAAGCAGATGCATTC+TGG - chr3_1:4390187-4390206 Msa0345240:CDS 50.0%
AGGGGAAGCAGATGCATTCT+GGG - chr3_1:4390188-4390207 Msa0345240:CDS 50.0%
!! TCTCTGATGGGTTGGGAGAA+CGG + chr3_1:4389247-4389266 None:intergenic 50.0%
!! TACCACGTCTCTGATGGGTT+GGG + chr3_1:4389254-4389273 None:intergenic 50.0%
! ATACCACGTCTCTGATGGGT+TGG + chr3_1:4389255-4389274 None:intergenic 50.0%
AGCGATACCACGTCTCTGAT+GGG + chr3_1:4389259-4389278 None:intergenic 50.0%
TAGCGATACCACGTCTCTGA+TGG + chr3_1:4389260-4389279 None:intergenic 50.0%
GATTGCCTCCCAAAACGACA+TGG - chr3_1:4389290-4389309 Msa0345240:intron 50.0%
!!! CTGTTCCATGTCGTTTTGGG+AGG + chr3_1:4389298-4389317 None:intergenic 50.0%
CTCCCAAAACGACATGGAAC+AGG - chr3_1:4389296-4389315 Msa0345240:intron 50.0%
AAGGGGAAGCAGATGCATTC+TGG - chr3_1:4390187-4390206 Msa0345240:CDS 50.0%
AGGGGAAGCAGATGCATTCT+GGG - chr3_1:4390188-4390207 Msa0345240:CDS 50.0%
AGCCTGTTGCCCTCTTCTCA+CGG - chr3_1:4389118-4389137 Msa0345240:CDS 55.0%
GAGTTGAGGCCGTGAGAAGA+GGG + chr3_1:4389130-4389149 None:intergenic 55.0%
! TGAGTTGAGGCCGTGAGAAG+AGG + chr3_1:4389131-4389150 None:intergenic 55.0%
GGGGAAGCAGATGCATTCTG+GGG - chr3_1:4390189-4390208 Msa0345240:CDS 55.0%
AGCCTGTTGCCCTCTTCTCA+CGG - chr3_1:4389118-4389137 Msa0345240:CDS 55.0%
GAGTTGAGGCCGTGAGAAGA+GGG + chr3_1:4389130-4389149 None:intergenic 55.0%
! TGAGTTGAGGCCGTGAGAAG+AGG + chr3_1:4389131-4389150 None:intergenic 55.0%
GGGGAAGCAGATGCATTCTG+GGG - chr3_1:4390189-4390208 Msa0345240:CDS 55.0%
!! ATGGGTTGGGAGAACGGCGA+GGG + chr3_1:4389241-4389260 None:intergenic 60.0%
CTCCCAACCCATCAGAGACG+TGG - chr3_1:4389249-4389268 Msa0345240:intron 60.0%
!! ATGGGTTGGGAGAACGGCGA+GGG + chr3_1:4389241-4389260 None:intergenic 60.0%
CTCCCAACCCATCAGAGACG+TGG - chr3_1:4389249-4389268 Msa0345240:intron 60.0%
GGCCGTGAGAAGAGGGCAAC+AGG + chr3_1:4389123-4389142 None:intergenic 65.0%
!! GATGGGTTGGGAGAACGGCG+AGG + chr3_1:4389242-4389261 None:intergenic 65.0%
GGCCGTGAGAAGAGGGCAAC+AGG + chr3_1:4389123-4389142 None:intergenic 65.0%
!! GATGGGTTGGGAGAACGGCG+AGG + chr3_1:4389242-4389261 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 4389048 4390240 4389048 ID=Msa0345240;Name=Msa0345240
chr3_1 mRNA 4389048 4390240 4389048 ID=Msa0345240-mRNA-1;Parent=Msa0345240;Name=Msa0345240-mRNA-1;_AED=0.07;_eAED=0.08;_QI=0|0|0|1|1|1|2|0|159
chr3_1 exon 4389971 4390240 4389971 ID=Msa0345240-mRNA-1:exon:1439;Parent=Msa0345240-mRNA-1
chr3_1 exon 4389048 4389257 4389048 ID=Msa0345240-mRNA-1:exon:1438;Parent=Msa0345240-mRNA-1
chr3_1 CDS 4389971 4390240 4389971 ID=Msa0345240-mRNA-1:cds;Parent=Msa0345240-mRNA-1
chr3_1 CDS 4389048 4389257 4389048 ID=Msa0345240-mRNA-1:cds;Parent=Msa0345240-mRNA-1
Gene Sequence

>Msa0345240

ATGTCTCAAGTGTGCATAAGAGAAGCAAACCTTTGCGACCTCTCTAAGATGTACAAACTCTTTGGTCTAAAGCCTGTTGCCCTCTTCTCACGGCCTCAACTCATTCACGTCGTAGAAGATATTAATGGTCACATCGTCGCCTACGTCCACTGTGAAATTCAAGAGGAAATTAGTGAAGGAGCTATTCATTCCCTCGCCGTTCTCCCAACCCATCAGAGACGTGGTATCGCTACTAAGCTTGTGATTGCCTCCCAAAACGACATGGAACAGATGTTTGGTTCTGAGTACGTCTCAGTGCATGTCAGAAAGAGCAACCATGCAGCTTTTAATTTGTTCAAAGAGACATTGGGATACAAGATTCATGGATTGGAGGCAAAGTTTTATGCTGATGATGGGGAGGATGCTTATGAAATGAGAAAGCAACTCAAGGGGAAGCAGATGCATTCTGGGGAGACTAAAGGGAATGCAAAAGCCACATGA

Protein sequence

>Msa0345240

MSQVCIREANLCDLSKMYKLFGLKPVALFSRPQLIHVVEDINGHIVAYVHCEIQEEISEGAIHSLAVLPTHQRRGIATKLVIASQNDMEQMFGSEYVSVHVRKSNHAAFNLFKETLGYKIHGLEAKFYADDGEDAYEMRKQLKGKQMHSGETKGNAKAT*