AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0347820

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0347820 MtrunA17_Chr3g0088851 81.373 102 13 1 1 102 1 96 7.10e-55 169
Msa0347820 MtrunA17_Chr3g0090311 46.584 161 69 6 6 155 2 156 1.85e-31 111
Msa0347820 MtrunA17_Chr3g0098461 40.496 121 65 3 37 155 37 152 1.01e-16 72.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0347820 AT5G13780.1 43.750 160 73 6 6 154 2 155 1.41e-27 102

Find 73 sgRNAs with CRISPR-Local

Find 239 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGAGAGAGAACCGAATAAA+TGG 0.287883 3_1:-9075648 None:intergenic
TTCCACAATCTGCCAATATT+CGG 0.289319 3_1:-9076119 None:intergenic
GTGGATCAGCTGCTATGTTT+TGG 0.322518 3_1:-9075724 None:intergenic
ATGCATACTTCAAAATATTA+AGG 0.338549 3_1:-9078593 None:intergenic
TCCACAATCTGCCAATATTC+GGG 0.342849 3_1:-9076118 None:intergenic
GAGCTGCAGAGTTGCTCTTC+CGG 0.345662 3_1:-9077018 None:intergenic
AATCCTTAACACCTAATCTA+TGG 0.347939 3_1:-9078457 None:intergenic
AAACCCGAAGTCCATTTATT+CGG 0.349829 3_1:+9075637 None:intergenic
TCTTGGTTTGTTTGAAAAGA+TGG 0.363394 3_1:+9077039 Msa0347820:CDS
GTTATATCAAATTGTTCATA+AGG 0.378902 3_1:-9078435 None:intergenic
AGAGGAAGTGGAAGAGGAAG+AGG 0.396334 3_1:+9076202 Msa0347820:CDS
CTCGTTACTCTCCCGAATAT+TGG 0.398957 3_1:+9076107 Msa0347820:CDS
CTGAGTAATGAGAGGAAATA+TGG 0.402178 3_1:+9078630 Msa0347820:three_prime_UTR
TAACCATAGATTAGGTGTTA+AGG 0.408808 3_1:+9078454 Msa0347820:three_prime_UTR
GTCGGAAACCGTACGGCCGC+CGG 0.414890 3_1:+9075750 Msa0347820:five_prime_UTR
CGGGTTAATCTTCAGTTTCA+AGG 0.415970 3_1:+9075770 Msa0347820:five_prime_UTR
GAACCGAATAAATGGACTTC+GGG 0.418967 3_1:-9075640 None:intergenic
AGTGGAAGAGGAAGAGGAAG+AGG 0.420722 3_1:+9076208 Msa0347820:CDS
ATTTGATATAACCATAGATT+AGG 0.426119 3_1:+9078446 Msa0347820:three_prime_UTR
CTCCTCATCATCAAAATACT+TGG 0.430558 3_1:-9077086 None:intergenic
TGCATAAGAGTCGCAACAAT+TGG 0.433948 3_1:+9076053 Msa0347820:CDS
CTCTAAAGAGGAAGTGGAAG+AGG 0.438354 3_1:+9076196 Msa0347820:CDS
GAAGAGGAAGAGGAAGAGGA+AGG 0.438753 3_1:+9076212 Msa0347820:CDS
CTCAATTATCACAGATTCAC+AGG 0.439603 3_1:-9078667 None:intergenic
CGCCCAAAACACCATGGAAA+AGG 0.453353 3_1:+9076316 Msa0347820:CDS
CTTGGTTTGTTTGAAAAGAT+GGG 0.459057 3_1:+9077040 Msa0347820:CDS
CCTCCTCTCTAAAGAGGAAG+TGG 0.459131 3_1:+9076190 Msa0347820:CDS
AACAATACAGTCGCTGCAAT+CGG 0.461342 3_1:-9076162 None:intergenic
AAGAGCAACTCTGCAGCTCT+TGG 0.462439 3_1:+9077022 Msa0347820:CDS
TGCTTACCTCCTCTCTAAAG+AGG 0.467882 3_1:+9076184 Msa0347820:CDS
CAATTGGAGAAGTATTAGAT+TGG 0.473450 3_1:+9076069 Msa0347820:CDS
AAGATGTCTCGGTGCACGTC+CGG 0.505757 3_1:+9076999 Msa0347820:CDS
TAATGAGAGGAAATATGGTT+GGG 0.510946 3_1:+9078635 Msa0347820:three_prime_UTR
CTTCCACTTCCTCTTTAGAG+AGG 0.515368 3_1:-9076193 None:intergenic
GAAGAGGAAGAGGAAGGGAA+TGG 0.522153 3_1:+9076218 Msa0347820:CDS
CGGCCGTACGGTTTCCGACA+CGG 0.533936 3_1:-9075746 None:intergenic
CCACTTCCTCTTTAGAGAGG+AGG 0.536526 3_1:-9076190 None:intergenic
CTCTCTTTACGCAACAGAGA+AGG 0.539863 3_1:+9075695 Msa0347820:five_prime_UTR
TCCCGAATATTGGCAGATTG+TGG 0.540617 3_1:+9076117 Msa0347820:CDS
GAGTGGCTCTCACAGTTAGG+TGG 0.542114 3_1:-9077152 None:intergenic
AAGAGGAAGAGGAAGAGGAA+GGG 0.544404 3_1:+9076213 Msa0347820:CDS
TCGGAAACCGTACGGCCGCC+GGG 0.544835 3_1:+9075751 Msa0347820:five_prime_UTR
GATTTAACATGTAGTACTGT+GGG 0.544885 3_1:+9078476 Msa0347820:three_prime_UTR
GGGTTAATCTTCAGTTTCAA+GGG 0.547826 3_1:+9075771 Msa0347820:five_prime_UTR
TTAATTTCAGGTCTCAAACA+TGG 0.547864 3_1:+9078215 Msa0347820:intron
AACATGGATTCTCATGCGGC+AGG 0.550095 3_1:+9078231 Msa0347820:three_prime_UTR
AGGAACAATGTCGTCAAAGA+AGG 0.558119 3_1:+9076028 Msa0347820:exon
AGATTAACCCGGCGGCCGTA+CGG 0.561465 3_1:-9075758 None:intergenic
TTACACACTTCTTGAGGTGA+AGG 0.565558 3_1:+9077063 Msa0347820:CDS
GTAATGAGAGGAAATATGGT+TGG 0.572852 3_1:+9078634 Msa0347820:three_prime_UTR
ATAGGTATGGTGAACAAAGA+GGG 0.575631 3_1:-9076258 None:intergenic
AGAACCGAATAAATGGACTT+CGG 0.580280 3_1:-9075641 None:intergenic
GATGGGTTACACACTTCTTG+AGG 0.581446 3_1:+9077057 Msa0347820:CDS
AGGAGGACGCTATTGTCTTG+TGG 0.587701 3_1:+9077104 Msa0347820:CDS
CCACCGTGTCGGAAACCGTA+CGG 0.591060 3_1:+9075743 Msa0347820:five_prime_UTR
CTTGAAACTGAAGATTAACC+CGG 0.595982 3_1:-9075769 None:intergenic
CTAACCATGAAACAGTTACA+AGG 0.600808 3_1:+9076008 Msa0347820:intron
ATGGATTCTCATGCGGCAGG+AGG 0.606412 3_1:+9078234 Msa0347820:three_prime_UTR
GATAGGTATGGTGAACAAAG+AGG 0.607709 3_1:-9076259 None:intergenic
GGATTTAACATGTAGTACTG+TGG 0.615437 3_1:+9078475 Msa0347820:three_prime_UTR
CATGGTAAATCGTTAGCTGA+AGG 0.616527 3_1:-9078261 None:intergenic
CACCGCCGCCCAAAACACCA+TGG 0.620119 3_1:+9076310 Msa0347820:CDS
CACCATACCTATCGTAAACG+TGG 0.628725 3_1:+9076269 Msa0347820:CDS
GGTGGGTGCTAAAGATGTCT+CGG 0.629911 3_1:+9076988 Msa0347820:intron
TGAATCTGTGATAATTGAGA+AGG 0.632046 3_1:+9078671 Msa0347820:three_prime_UTR
AAAATATACAAAGTACTACA+TGG 0.635553 3_1:-9078279 None:intergenic
CTCAAACATGGATTCTCATG+CGG 0.636062 3_1:+9078227 Msa0347820:three_prime_UTR
AGTGAGTGCTGAGTAATGAG+AGG 0.650346 3_1:+9078622 Msa0347820:three_prime_UTR
TACCACGTTTACGATAGGTA+TGG 0.651983 3_1:-9076271 None:intergenic
TAGCAGCTGATCCACCGTGT+CGG 0.668395 3_1:+9075732 Msa0347820:five_prime_UTR
AGCGATACCACGTTTACGAT+AGG 0.681782 3_1:-9076276 None:intergenic
CCGTACGGTTTCCGACACGG+TGG 0.766152 3_1:-9075743 None:intergenic
GAAACTGAAGATTAACCCGG+CGG 0.769186 3_1:-9075766 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCTGTTATAATTTTGTTTTT+TGG + chr3_1:9076357-9076376 Msa0347820:intron 15.0%
!!! ATAATTTTGTTTTTTGGATT+AGG + chr3_1:9076363-9076382 Msa0347820:intron 15.0%
!! AGTTAGAAAGAAAGAAATTA+GGG - chr3_1:9075897-9075916 None:intergenic 20.0%
!! AAGTTAGAAAGAAAGAAATT+AGG - chr3_1:9075898-9075917 None:intergenic 20.0%
!!! TTTGGATTTTTAAAGAGAAT+GGG - chr3_1:9075968-9075987 None:intergenic 20.0%
!!! TTTTGGATTTTTAAAGAGAA+TGG - chr3_1:9075969-9075988 None:intergenic 20.0%
!!! TTCTAAAGATTGTTATGTTT+TGG - chr3_1:9075986-9076005 None:intergenic 20.0%
!! GAAAATAATTGAAACTTTGA+TGG + chr3_1:9077208-9077227 Msa0347820:intron 20.0%
!!! TTGATAGTCAATAATACTTA+GGG + chr3_1:9077311-9077330 Msa0347820:intron 20.0%
!! TAATCACAATTCATTAAACA+AGG - chr3_1:9078127-9078146 None:intergenic 20.0%
!!! AAAATATACAAAGTACTACA+TGG - chr3_1:9078282-9078301 None:intergenic 20.0%
!!! ACTTTGTATATTTTCTAGTA+TGG + chr3_1:9078288-9078307 Msa0347820:intron 20.0%
!!! ACTTTGTATATTTTCTAGTA+TGG + chr3_1:9078288-9078307 Msa0347820:intron 20.0%
!! GTTATATCAAATTGTTCATA+AGG - chr3_1:9078438-9078457 None:intergenic 20.0%
!! ATTTGATATAACCATAGATT+AGG + chr3_1:9078446-9078465 Msa0347820:three_prime_UTR 20.0%
!! ATGCATACTTCAAAATATTA+AGG - chr3_1:9078596-9078615 None:intergenic 20.0%
! AAGCATTACATACGAATTTA+AGG - chr3_1:9075811-9075830 None:intergenic 25.0%
!! TTTCTTCTTGTTAGTGATTT+TGG + chr3_1:9076737-9076756 Msa0347820:intron 25.0%
! GAAAACCAAAAGAAAAATCT+CGG - chr3_1:9076820-9076839 None:intergenic 25.0%
!!! AGACCTTTTTTTGATGTTTT+TGG + chr3_1:9076909-9076928 Msa0347820:intron 25.0%
! CAACCAAAAACATCAAAAAA+AGG - chr3_1:9076915-9076934 None:intergenic 25.0%
! ACATTGTAACAGACTATTAA+TGG - chr3_1:9076954-9076973 None:intergenic 25.0%
! TCAAGAAAATGGAAAAAGAT+GGG - chr3_1:9077179-9077198 None:intergenic 25.0%
! TTCAAGAAAATGGAAAAAGA+TGG - chr3_1:9077180-9077199 None:intergenic 25.0%
! TCACAAAACATTCAAGAAAA+TGG - chr3_1:9077190-9077209 None:intergenic 25.0%
!! CTTGATAGTCAATAATACTT+AGG + chr3_1:9077310-9077329 Msa0347820:intron 25.0%
! TTAACTACTAGTACCTTATA+AGG + chr3_1:9077675-9077694 Msa0347820:intron 25.0%
! AAATATACTGTTTGAGTATG+TGG - chr3_1:9077747-9077766 None:intergenic 25.0%
! CATTTGTTCTCTTAATTGAT+TGG + chr3_1:9077964-9077983 Msa0347820:intron 25.0%
! GAAAATATGCAAGTAGTAAA+AGG - chr3_1:9078102-9078121 None:intergenic 25.0%
!! AATTAAATCATGGTGTTTTC+TGG + chr3_1:9078190-9078209 Msa0347820:intron 25.0%
!!! TGTTTTCTGGATTTAATTTC+AGG + chr3_1:9078203-9078222 Msa0347820:intron 25.0%
!!! AGTATGGTATCTTTTAAAGT+AGG + chr3_1:9078304-9078323 Msa0347820:intron 25.0%
!!! GTATGGTATCTTTTAAAGTA+GGG + chr3_1:9078305-9078324 Msa0347820:intron 25.0%
!!! TATGGTATCTTTTAAAGTAG+GGG + chr3_1:9078306-9078325 Msa0347820:intron 25.0%
!!! AGTATGGTATCTTTTAAAGT+AGG + chr3_1:9078304-9078323 Msa0347820:intron 25.0%
!!! GTATGGTATCTTTTAAAGTA+GGG + chr3_1:9078305-9078324 Msa0347820:intron 25.0%
! AAAAATTATCACAGTACTTG+CGG - chr3_1:9078405-9078424 None:intergenic 25.0%
!!! AGTTTGGTATCTTTTAAAGT+AGG + chr3_1:9078508-9078527 Msa0347820:three_prime_UTR 25.0%
!!! GTTTGGTATCTTTTAAAGTA+GGG + chr3_1:9078509-9078528 Msa0347820:three_prime_UTR 25.0%
AGGGTTTCAATAGAAATTTG+GGG - chr3_1:9075848-9075867 None:intergenic 30.0%
GAGGGTTTCAATAGAAATTT+GGG - chr3_1:9075849-9075868 None:intergenic 30.0%
TGAGGGTTTCAATAGAAATT+TGG - chr3_1:9075850-9075869 None:intergenic 30.0%
TGATTTCTCAGTCAAAATGA+GGG - chr3_1:9075867-9075886 None:intergenic 30.0%
ATGATTTCTCAGTCAAAATG+AGG - chr3_1:9075868-9075887 None:intergenic 30.0%
TTGTAACTGTTTCATGGTTA+GGG - chr3_1:9076009-9076028 None:intergenic 30.0%
!! CAATTGGAGAAGTATTAGAT+TGG + chr3_1:9076069-9076088 Msa0347820:CDS 30.0%
TCCATACATAACCAGTTAAA+AGG - chr3_1:9076428-9076447 None:intergenic 30.0%
! TATGTATGGAGTTGGTTTAA+TGG + chr3_1:9076438-9076457 Msa0347820:intron 30.0%
AGAAAACAGAAAATCATCAC+AGG - chr3_1:9076566-9076585 None:intergenic 30.0%
! TGTGATGATTTTCTGTTTTC+TGG + chr3_1:9076565-9076584 Msa0347820:intron 30.0%
! CTAGGATTATCTTTTATTCC+TGG + chr3_1:9076609-9076628 Msa0347820:intron 30.0%
! TTATCTTTTATTCCTGGAAG+TGG + chr3_1:9076615-9076634 Msa0347820:intron 30.0%
!! TTTTCTTGTTTCCTAGTCTT+GGG + chr3_1:9076835-9076854 Msa0347820:intron 30.0%
ACAATGTGTAATTTGACTGA+AGG + chr3_1:9076967-9076986 Msa0347820:intron 30.0%
!! TCTTGGTTTGTTTGAAAAGA+TGG + chr3_1:9077039-9077058 Msa0347820:CDS 30.0%
!! CTTGGTTTGTTTGAAAAGAT+GGG + chr3_1:9077040-9077059 Msa0347820:CDS 30.0%
AAGAAAATGGAAAAAGATGG+GGG - chr3_1:9077177-9077196 None:intergenic 30.0%
CAAGAAAATGGAAAAAGATG+GGG - chr3_1:9077178-9077197 None:intergenic 30.0%
! ATAATTGAAACTTTGATGGC+TGG + chr3_1:9077212-9077231 Msa0347820:intron 30.0%
CTGGTTTAATATTGACATGA+TGG + chr3_1:9077231-9077250 Msa0347820:intron 30.0%
! TTTTGATCGCTATCTTTTTG+TGG + chr3_1:9077348-9077367 Msa0347820:intron 30.0%
TTATCCAAGGATGAAAAAGT+CGG - chr3_1:9077396-9077415 None:intergenic 30.0%
! TGTAAAGAGCATATTATCCA+AGG - chr3_1:9077409-9077428 None:intergenic 30.0%
TGGATAATATGCTCTTTACA+TGG + chr3_1:9077409-9077428 Msa0347820:intron 30.0%
!! TTTCTTGAGTGTAGTTCTAA+TGG + chr3_1:9077522-9077541 Msa0347820:intron 30.0%
!! TTCTTGAGTGTAGTTCTAAT+GGG + chr3_1:9077523-9077542 Msa0347820:intron 30.0%
!! TGTTGCTTATTGATTTCGAA+TGG + chr3_1:9077611-9077630 Msa0347820:intron 30.0%
TAACGTCATGTAAAGTAACA+CGG - chr3_1:9077658-9077677 None:intergenic 30.0%
TCTAAAAAGTGGTCCTTATA+AGG - chr3_1:9077691-9077710 None:intergenic 30.0%
TGTTTCCACTATCTAAAAAG+TGG - chr3_1:9077702-9077721 None:intergenic 30.0%
!! TTTGTCTTTTTCACAGATCT+AGG + chr3_1:9077795-9077814 Msa0347820:intron 30.0%
TTAATCCTTTCAGCTTTCTT+CGG + chr3_1:9077869-9077888 Msa0347820:intron 30.0%
GCTTATTGAGGATTAAGATT+AGG + chr3_1:9078010-9078029 Msa0347820:intron 30.0%
ATTGAGGATTAAGATTAGGT+TGG + chr3_1:9078014-9078033 Msa0347820:intron 30.0%
TTGAGGATTAAGATTAGGTT+GGG + chr3_1:9078015-9078034 Msa0347820:intron 30.0%
TGCTGATGTCAATTAAATCA+TGG + chr3_1:9078180-9078199 Msa0347820:intron 30.0%
! TTAATTTCAGGTCTCAAACA+TGG + chr3_1:9078215-9078234 Msa0347820:intron 30.0%
! CCAAAATATCAAAGTACTTG+TGG - chr3_1:9078335-9078354 None:intergenic 30.0%
! CCACAAGTACTTTGATATTT+TGG + chr3_1:9078332-9078351 Msa0347820:intron 30.0%
TAACCATAGATTAGGTGTTA+AGG + chr3_1:9078454-9078473 Msa0347820:three_prime_UTR 30.0%
AATCCTTAACACCTAATCTA+TGG - chr3_1:9078460-9078479 None:intergenic 30.0%
GATTTAACATGTAGTACTGT+GGG + chr3_1:9078476-9078495 Msa0347820:three_prime_UTR 30.0%
TAATGAGAGGAAATATGGTT+GGG + chr3_1:9078635-9078654 Msa0347820:three_prime_UTR 30.0%
! AGAACCGAATAAATGGACTT+CGG - chr3_1:9075644-9075663 None:intergenic 35.0%
CTTGAAACTGAAGATTAACC+CGG - chr3_1:9075772-9075791 None:intergenic 35.0%
GGGTTAATCTTCAGTTTCAA+GGG + chr3_1:9075771-9075790 Msa0347820:five_prime_UTR 35.0%
GGGTTTCAATAGAAATTTGG+GGG - chr3_1:9075847-9075866 None:intergenic 35.0%
CTTGTAACTGTTTCATGGTT+AGG - chr3_1:9076010-9076029 None:intergenic 35.0%
CTAACCATGAAACAGTTACA+AGG + chr3_1:9076008-9076027 Msa0347820:intron 35.0%
TGTTCCTTGTAACTGTTTCA+TGG - chr3_1:9076015-9076034 None:intergenic 35.0%
TTCCACAATCTGCCAATATT+CGG - chr3_1:9076122-9076141 None:intergenic 35.0%
ATAGGTATGGTGAACAAAGA+GGG - chr3_1:9076261-9076280 None:intergenic 35.0%
! AACCTTTTCCATGGTGTTTT+GGG - chr3_1:9076321-9076340 None:intergenic 35.0%
! TAACCTTTTCCATGGTGTTT+TGG - chr3_1:9076322-9076341 None:intergenic 35.0%
AAAACACCATGGAAAAGGTT+AGG + chr3_1:9076321-9076340 Msa0347820:intron 35.0%
! AACAAACCTAACCTTTTCCA+TGG - chr3_1:9076330-9076349 None:intergenic 35.0%
!! GCCTTTTAACTGGTTATGTA+TGG + chr3_1:9076424-9076443 Msa0347820:intron 35.0%
TAACTGGTTATGTATGGAGT+TGG + chr3_1:9076430-9076449 Msa0347820:intron 35.0%
! ATGGAGTTGGTTTAATGGTT+AGG + chr3_1:9076443-9076462 Msa0347820:intron 35.0%
TAATGGTTAGGTATGAAGCT+TGG + chr3_1:9076455-9076474 Msa0347820:intron 35.0%
AACAAAAAACCTAGCCTACT+CGG - chr3_1:9076509-9076528 None:intergenic 35.0%
! TTTTCAGATAGATCACACAG+TGG - chr3_1:9076543-9076562 None:intergenic 35.0%
! CTTCTTGTTAGTGATTTTGG+TGG + chr3_1:9076740-9076759 Msa0347820:intron 35.0%
!! TTGTTAGTGATTTTGGTGGT+TGG + chr3_1:9076744-9076763 Msa0347820:intron 35.0%
!! TTTGTGTAGTTAGTCTTGTC+TGG + chr3_1:9076778-9076797 Msa0347820:intron 35.0%
AACCAAAAGAAAAATCTCGG+AGG - chr3_1:9076817-9076836 None:intergenic 35.0%
!! GTTTTCTTGTTTCCTAGTCT+TGG + chr3_1:9076834-9076853 Msa0347820:intron 35.0%
!! TTTCTTGTTTCCTAGTCTTG+GGG + chr3_1:9076836-9076855 Msa0347820:intron 35.0%
ATGTGTAATTTGACTGAAGG+TGG + chr3_1:9076970-9076989 Msa0347820:intron 35.0%
TGTGTAATTTGACTGAAGGT+GGG + chr3_1:9076971-9076990 Msa0347820:intron 35.0%
CTCCTCATCATCAAAATACT+TGG - chr3_1:9077089-9077108 None:intergenic 35.0%
!!! CAAGTATTTTGATGATGAGG+AGG + chr3_1:9077087-9077106 Msa0347820:CDS 35.0%
ATATTGACATGATGGCAATG+TGG + chr3_1:9077239-9077258 Msa0347820:intron 35.0%
AGTCGGTTAAATACTAGCAT+TGG - chr3_1:9077379-9077398 None:intergenic 35.0%
!!! TTAACCGACTTTTTCATCCT+TGG + chr3_1:9077389-9077408 Msa0347820:intron 35.0%
TATGAGCAATTTAGCTGTGT+AGG + chr3_1:9077449-9077468 Msa0347820:intron 35.0%
ATGAGCAATTTAGCTGTGTA+GGG + chr3_1:9077450-9077469 Msa0347820:intron 35.0%
!! TGCTTATTGATTTCGAATGG+AGG + chr3_1:9077614-9077633 Msa0347820:intron 35.0%
!!! AAGGACCACTTTTTAGATAG+TGG + chr3_1:9077694-9077713 Msa0347820:intron 35.0%
AAAGCTGAAAGGATTAAAGC+TGG - chr3_1:9077866-9077885 None:intergenic 35.0%
ATAAACCGAAGAAAGCTGAA+AGG - chr3_1:9077877-9077896 None:intergenic 35.0%
! TTTTCTGACTCTGCTCTTAT+TGG + chr3_1:9077897-9077916 Msa0347820:intron 35.0%
ATGTTGCTCATTGCTTATTG+AGG + chr3_1:9077998-9078017 Msa0347820:intron 35.0%
! TGAGGATTAAGATTAGGTTG+GGG + chr3_1:9078016-9078035 Msa0347820:intron 35.0%
!!! TGGTATCTTTTAAAGTAGGG+TGG + chr3_1:9078308-9078327 Msa0347820:intron 35.0%
GGATTTAACATGTAGTACTG+TGG + chr3_1:9078475-9078494 Msa0347820:three_prime_UTR 35.0%
!!! TGGTATCTTTTAAAGTAGGG+TGG + chr3_1:9078308-9078327 Msa0347820:intron 35.0%
CTGAGTAATGAGAGGAAATA+TGG + chr3_1:9078630-9078649 Msa0347820:three_prime_UTR 35.0%
GTAATGAGAGGAAATATGGT+TGG + chr3_1:9078634-9078653 Msa0347820:three_prime_UTR 35.0%
CTCAATTATCACAGATTCAC+AGG - chr3_1:9078670-9078689 None:intergenic 35.0%
GAGAGAGAGAACCGAATAAA+TGG - chr3_1:9075651-9075670 None:intergenic 40.0%
CGGGTTAATCTTCAGTTTCA+AGG + chr3_1:9075770-9075789 Msa0347820:five_prime_UTR 40.0%
AGGAACAATGTCGTCAAAGA+AGG + chr3_1:9076028-9076047 Msa0347820:exon 40.0%
TGCATAAGAGTCGCAACAAT+TGG + chr3_1:9076053-9076072 Msa0347820:CDS 40.0%
TCCACAATCTGCCAATATTC+GGG - chr3_1:9076121-9076140 None:intergenic 40.0%
AACAATACAGTCGCTGCAAT+CGG - chr3_1:9076165-9076184 None:intergenic 40.0%
GATAGGTATGGTGAACAAAG+AGG - chr3_1:9076262-9076281 None:intergenic 40.0%
TACCACGTTTACGATAGGTA+TGG - chr3_1:9076274-9076293 None:intergenic 40.0%
! TGGATTAGGTTTGTCTCTTG+TGG + chr3_1:9076377-9076396 Msa0347820:intron 40.0%
GGATTAGGTTTGTCTCTTGT+GGG + chr3_1:9076378-9076397 Msa0347820:intron 40.0%
! TGTGGTATAGGCCTTTTAAC+TGG + chr3_1:9076414-9076433 Msa0347820:intron 40.0%
GTTAGGTATGAAGCTTGGTT+CGG + chr3_1:9076460-9076479 Msa0347820:intron 40.0%
!! TTGGTTCGGTTGTTTCGTTA+GGG + chr3_1:9076474-9076493 Msa0347820:intron 40.0%
GGTAGAATCTGAGTTTCACT+AGG + chr3_1:9076586-9076605 Msa0347820:intron 40.0%
AATCTGAGTTTCACTAGGCT+AGG + chr3_1:9076591-9076610 Msa0347820:intron 40.0%
TATTCCTGGAAGTGGTTCTT+TGG + chr3_1:9076623-9076642 Msa0347820:intron 40.0%
AAAGGGAGTGGCAATGATTA+GGG - chr3_1:9076655-9076674 None:intergenic 40.0%
TAAAGGGAGTGGCAATGATT+AGG - chr3_1:9076656-9076675 None:intergenic 40.0%
AAACCAATTCTCTGTGGGAA+GGG + chr3_1:9076689-9076708 Msa0347820:intron 40.0%
! GTCCTCCGAGATTTTTCTTT+TGG + chr3_1:9076812-9076831 Msa0347820:intron 40.0%
AAAATCCATACCCCAAGACT+AGG - chr3_1:9076849-9076868 None:intergenic 40.0%
! TTGGGGTATGGATTTTCTCT+AGG + chr3_1:9076853-9076872 Msa0347820:intron 40.0%
TTACACACTTCTTGAGGTGA+AGG + chr3_1:9077063-9077082 Msa0347820:CDS 40.0%
!!! GGCCAAGTATTTTGATGATG+AGG + chr3_1:9077084-9077103 Msa0347820:CDS 40.0%
CAGTGCTTTCTATTTCAGTG+TGG + chr3_1:9077575-9077594 Msa0347820:intron 40.0%
ACCAGACAGAGCACAATATA+CGG - chr3_1:9077843-9077862 None:intergenic 40.0%
!!! AGATTAGGTTGGGGTTTTGT+TGG + chr3_1:9078025-9078044 Msa0347820:intron 40.0%
GTTGGACTTAGACTAACACT+TGG + chr3_1:9078043-9078062 Msa0347820:intron 40.0%
ACTTGGAGTAAAAAGTCCTG+AGG + chr3_1:9078060-9078079 Msa0347820:intron 40.0%
AAACCATAGCTATGATCCTC+AGG - chr3_1:9078079-9078098 None:intergenic 40.0%
CTCAAACATGGATTCTCATG+CGG + chr3_1:9078227-9078246 Msa0347820:three_prime_UTR 40.0%
CATGGTAAATCGTTAGCTGA+AGG - chr3_1:9078264-9078283 None:intergenic 40.0%
!!! GGTATCTTTTAAAGTAGGGG+TGG + chr3_1:9078309-9078328 Msa0347820:intron 40.0%
!! CTGTGGGATTTTTCGAAGTT+TGG + chr3_1:9078492-9078511 Msa0347820:three_prime_UTR 40.0%
CTCTCTTTACGCAACAGAGA+AGG + chr3_1:9075695-9075714 Msa0347820:five_prime_UTR 45.0%
! GTGGATCAGCTGCTATGTTT+TGG - chr3_1:9075727-9075746 None:intergenic 45.0%
GAAACTGAAGATTAACCCGG+CGG - chr3_1:9075769-9075788 None:intergenic 45.0%
CTCGTTACTCTCCCGAATAT+TGG + chr3_1:9076107-9076126 Msa0347820:CDS 45.0%
TCCCGAATATTGGCAGATTG+TGG + chr3_1:9076117-9076136 Msa0347820:CDS 45.0%
! GAAAAAGCTTGTTCACGTCG+CGG + chr3_1:9076139-9076158 Msa0347820:CDS 45.0%
! TGCTTACCTCCTCTCTAAAG+AGG + chr3_1:9076184-9076203 Msa0347820:CDS 45.0%
! CTTCCACTTCCTCTTTAGAG+AGG - chr3_1:9076196-9076215 None:intergenic 45.0%
! CTCTAAAGAGGAAGTGGAAG+AGG + chr3_1:9076196-9076215 Msa0347820:CDS 45.0%
AAGAGGAAGAGGAAGAGGAA+GGG + chr3_1:9076213-9076232 Msa0347820:CDS 45.0%
CACCATACCTATCGTAAACG+TGG + chr3_1:9076269-9076288 Msa0347820:CDS 45.0%
AGCGATACCACGTTTACGAT+AGG - chr3_1:9076279-9076298 None:intergenic 45.0%
!! CTTTTCCATGGTGTTTTGGG+CGG - chr3_1:9076318-9076337 None:intergenic 45.0%
!! TCTCTTGTGGGTTAGCGTAT+AGG + chr3_1:9076390-9076409 Msa0347820:intron 45.0%
!! CTCTTGTGGGTTAGCGTATA+GGG + chr3_1:9076391-9076410 Msa0347820:intron 45.0%
TAGCGTATAGGGTGTGGTAT+AGG + chr3_1:9076402-9076421 Msa0347820:intron 45.0%
!! CTTGGTTCGGTTGTTTCGTT+AGG + chr3_1:9076473-9076492 Msa0347820:intron 45.0%
!! TGGTTCGGTTGTTTCGTTAG+GGG + chr3_1:9076475-9076494 Msa0347820:intron 45.0%
TTCACCAAAGAACCACTTCC+AGG - chr3_1:9076630-9076649 None:intergenic 45.0%
ATTGCCACTCCCTTTATGAG+TGG + chr3_1:9076660-9076679 Msa0347820:intron 45.0%
TTGCCACTCCCTTTATGAGT+GGG + chr3_1:9076661-9076680 Msa0347820:intron 45.0%
TTTCGCCTCCCACTCATAAA+GGG - chr3_1:9076672-9076691 None:intergenic 45.0%
AGGCGAAACCAATTCTCTGT+GGG + chr3_1:9076684-9076703 Msa0347820:intron 45.0%
GAAACCAATTCTCTGTGGGA+AGG + chr3_1:9076688-9076707 Msa0347820:intron 45.0%
AAACCCTTCCCACAGAGAAT+TGG - chr3_1:9076695-9076714 None:intergenic 45.0%
! TTCTCTGTGGGAAGGGTTTT+TGG + chr3_1:9076696-9076715 Msa0347820:intron 45.0%
!! TGTTTCCTAGTCTTGGGGTA+TGG + chr3_1:9076841-9076860 Msa0347820:intron 45.0%
!! GATGGGTTACACACTTCTTG+AGG + chr3_1:9077057-9077076 Msa0347820:CDS 45.0%
AATGGAAAAAGATGGGGGAG+TGG - chr3_1:9077172-9077191 None:intergenic 45.0%
AGCAATTTAGCTGTGTAGGG+TGG + chr3_1:9077453-9077472 Msa0347820:intron 45.0%
GCAATTTAGCTGTGTAGGGT+GGG + chr3_1:9077454-9077473 Msa0347820:intron 45.0%
TCCGTATATTGTGCTCTGTC+TGG + chr3_1:9077839-9077858 Msa0347820:intron 45.0%
AGTCCTGAGGATCATAGCTA+TGG + chr3_1:9078073-9078092 Msa0347820:intron 45.0%
!! AGTGAGTGCTGAGTAATGAG+AGG + chr3_1:9078622-9078641 Msa0347820:three_prime_UTR 45.0%
! CCACTTCCTCTTTAGAGAGG+AGG - chr3_1:9076193-9076212 None:intergenic 50.0%
! CCTCCTCTCTAAAGAGGAAG+TGG + chr3_1:9076190-9076209 Msa0347820:CDS 50.0%
AGAGGAAGTGGAAGAGGAAG+AGG + chr3_1:9076202-9076221 Msa0347820:CDS 50.0%
AGTGGAAGAGGAAGAGGAAG+AGG + chr3_1:9076208-9076227 Msa0347820:CDS 50.0%
GAAGAGGAAGAGGAAGAGGA+AGG + chr3_1:9076212-9076231 Msa0347820:CDS 50.0%
GAAGAGGAAGAGGAAGGGAA+TGG + chr3_1:9076218-9076237 Msa0347820:CDS 50.0%
CGCCCAAAACACCATGGAAA+AGG + chr3_1:9076316-9076335 Msa0347820:CDS 50.0%
! TTCGGTTGTTTCGTTAGGGG+TGG + chr3_1:9076478-9076497 Msa0347820:intron 50.0%
! TCGGTTGTTTCGTTAGGGGT+GGG + chr3_1:9076479-9076498 Msa0347820:intron 50.0%
TTGTTTCGTTAGGGGTGGGA+AGG + chr3_1:9076483-9076502 Msa0347820:intron 50.0%
GTTTCGCCTCCCACTCATAA+AGG - chr3_1:9076673-9076692 None:intergenic 50.0%
GAGGCGAAACCAATTCTCTG+TGG + chr3_1:9076683-9076702 Msa0347820:intron 50.0%
GTCTTGTCTGGTGAGTGTTC+TGG + chr3_1:9076790-9076809 Msa0347820:intron 50.0%
TACAACAGCTGCAGGTCTCA+AGG - chr3_1:9076888-9076907 None:intergenic 50.0%
AAGGTCTGTACAACAGCTGC+AGG - chr3_1:9076896-9076915 None:intergenic 50.0%
GGTGGGTGCTAAAGATGTCT+CGG + chr3_1:9076988-9077007 Msa0347820:intron 50.0%
AAGAGCAACTCTGCAGCTCT+TGG + chr3_1:9077022-9077041 Msa0347820:CDS 50.0%
AGGAGGACGCTATTGTCTTG+TGG + chr3_1:9077104-9077123 Msa0347820:CDS 50.0%
AACATGGATTCTCATGCGGC+AGG + chr3_1:9078231-9078250 Msa0347820:three_prime_UTR 50.0%
TAGCAGCTGATCCACCGTGT+CGG + chr3_1:9075732-9075751 Msa0347820:five_prime_UTR 55.0%
!! TTCCATGGTGTTTTGGGCGG+CGG - chr3_1:9076315-9076334 None:intergenic 55.0%
!! GTGGGTTAGCGTATAGGGTG+TGG + chr3_1:9076396-9076415 Msa0347820:intron 55.0%
CCTCCCACTCATAAAGGGAG+TGG - chr3_1:9076667-9076686 None:intergenic 55.0%
CCACTCCCTTTATGAGTGGG+AGG + chr3_1:9076664-9076683 Msa0347820:intron 55.0%
! AAGATGTCTCGGTGCACGTC+CGG + chr3_1:9076999-9077018 Msa0347820:CDS 55.0%
GAGCTGCAGAGTTGCTCTTC+CGG - chr3_1:9077021-9077040 None:intergenic 55.0%
GAGTGGCTCTCACAGTTAGG+TGG - chr3_1:9077155-9077174 None:intergenic 55.0%
! GTGGGCGTGAGGTCAGATTT+TGG + chr3_1:9077472-9077491 Msa0347820:intron 55.0%
ATGGATTCTCATGCGGCAGG+AGG + chr3_1:9078234-9078253 Msa0347820:three_prime_UTR 55.0%
CCACCGTGTCGGAAACCGTA+CGG + chr3_1:9075743-9075762 Msa0347820:five_prime_UTR 60.0%
AGATTAACCCGGCGGCCGTA+CGG - chr3_1:9075761-9075780 None:intergenic 60.0%
GGGGAGTGGCTCTCACAGTT+AGG - chr3_1:9077158-9077177 None:intergenic 60.0%
CCGTACGGTTTCCGACACGG+TGG - chr3_1:9075746-9075765 None:intergenic 65.0%
CGGCCGTACGGTTTCCGACA+CGG - chr3_1:9075749-9075768 None:intergenic 65.0%
CACCGCCGCCCAAAACACCA+TGG + chr3_1:9076310-9076329 Msa0347820:CDS 65.0%
TAGGGGTGGGAAGGCCGAGT+AGG + chr3_1:9076492-9076511 Msa0347820:intron 65.0%
GTGGGAAGGCCGAGTAGGCT+AGG + chr3_1:9076497-9076516 Msa0347820:intron 65.0%
AGCTGTGTAGGGTGGGCGTG+AGG + chr3_1:9077461-9077480 Msa0347820:intron 65.0%
GTCGGAAACCGTACGGCCGC+CGG + chr3_1:9075750-9075769 Msa0347820:five_prime_UTR 70.0%
TCGGAAACCGTACGGCCGCC+GGG + chr3_1:9075751-9075770 Msa0347820:five_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 9075641 9078690 9075641 ID=Msa0347820;Name=Msa0347820
chr3_1 mRNA 9075641 9078690 9075641 ID=Msa0347820-mRNA-1;Parent=Msa0347820;Name=Msa0347820-mRNA-1;_AED=0.07;_eAED=0.07;_QI=164|1|1|1|0.25|0|5|419|157
chr3_1 exon 9075641 9075792 9075641 ID=Msa0347820-mRNA-1:exon:2632;Parent=Msa0347820-mRNA-1
chr3_1 exon 9076023 9076337 9076023 ID=Msa0347820-mRNA-1:exon:2633;Parent=Msa0347820-mRNA-1
chr3_1 exon 9076989 9077161 9076989 ID=Msa0347820-mRNA-1:exon:2634;Parent=Msa0347820-mRNA-1
chr3_1 exon 9078225 9078304 9078225 ID=Msa0347820-mRNA-1:exon:2635;Parent=Msa0347820-mRNA-1
chr3_1 exon 9078354 9078690 9078354 ID=Msa0347820-mRNA-1:exon:2636;Parent=Msa0347820-mRNA-1
chr3_1 five_prime_UTR 9075641 9075792 9075641 ID=Msa0347820-mRNA-1:five_prime_utr;Parent=Msa0347820-mRNA-1
chr3_1 five_prime_UTR 9076023 9076034 9076023 ID=Msa0347820-mRNA-1:five_prime_utr;Parent=Msa0347820-mRNA-1
chr3_1 CDS 9076035 9076337 9076035 ID=Msa0347820-mRNA-1:cds;Parent=Msa0347820-mRNA-1
chr3_1 CDS 9076989 9077159 9076989 ID=Msa0347820-mRNA-1:cds;Parent=Msa0347820-mRNA-1
chr3_1 three_prime_UTR 9077160 9077161 9077160 ID=Msa0347820-mRNA-1:three_prime_utr;Parent=Msa0347820-mRNA-1
chr3_1 three_prime_UTR 9078225 9078304 9078225 ID=Msa0347820-mRNA-1:three_prime_utr;Parent=Msa0347820-mRNA-1
chr3_1 three_prime_UTR 9078354 9078690 9078354 ID=Msa0347820-mRNA-1:three_prime_utr;Parent=Msa0347820-mRNA-1
Gene Sequence

>Msa0347820

ATGTCGTCAAAGAAGGTGTGCATAAGAGTCGCAACAATTGGAGAAGTATTAGATTGGAACAGTAACAACAATCTCGTTACTCTCCCGAATATTGGCAGATTGTGGAAAAAGCTTGTTCACGTCGCGGCCGATTGCAGCGACTGTATTGTTGCTTACCTCCTCTCTAAAGAGGAAGTGGAAGAGGAAGAGGAAGAGGAAGGGAATGGTCACTTTTGTATCGCTTCCCTCTTTGTTCACCATACCTATCGTAAACGTGGTATCGCTACTGATCTTATCACCGCCGCCCAAAACACCATGGAAAAGGTGGGTGCTAAAGATGTCTCGGTGCACGTCCGGAAGAGCAACTCTGCAGCTCTTGGTTTGTTTGAAAAGATGGGTTACACACTTCTTGAGGTGAAGGCCAAGTATTTTGATGATGAGGAGGACGCTATTGTCTTGTGGAAGAAACTCAACGAGAAGCATATAGCCACCTAA
Protein sequence

>Msa0347820

MSSKKVCIRVATIGEVLDWNSNNNLVTLPNIGRLWKKLVHVAADCSDCIVAYLLSKEEVEEEEEEEGNGHFCIASLFVHHTYRKRGIATDLITAAQNTMEKVGAKDVSVHVRKSNSAALGLFEKMGYTLLEVKAKYFDDEEDAIVLWKKLNEKHIAT*