AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0347960

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0347960 MtrunA17_Chr4g0036071 83.636 55 9 0 1 55 348 402 1.57e-22 89.0
Msa0347960 MtrunA17_Chr2g0306581 83.333 48 8 0 1 48 1 48 3.51e-21 80.1
Msa0347960 MtrunA17_Chr2g0329551 69.091 55 17 0 1 55 209 263 4.95e-18 76.3
Msa0347960 MtrunA17_Chr6g0463571 72.549 51 14 0 1 51 139 189 1.85e-17 73.9
Msa0347960 MtrunA17_Chr5g0432701 69.091 55 17 0 1 55 147 201 2.50e-17 73.9
Msa0347960 MtrunA17_Chr3g0107491 69.091 55 17 0 1 55 165 219 3.79e-17 73.6
Msa0347960 MtrunA17_Chr1g0156051 77.778 45 10 0 1 45 375 419 2.15e-15 68.9
Msa0347960 MtrunA17_Chr3g0128781 60.000 55 22 0 1 55 96 150 2.20e-15 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0347960 AT4G38900.2 76.364 55 13 0 1 55 382 436 3.13e-21 85.9
Msa0347960 AT4G38900.3 76.364 55 13 0 1 55 382 436 3.13e-21 85.9
Msa0347960 AT2G21230.4 75.926 54 13 0 2 55 359 412 6.82e-20 82.0
Msa0347960 AT2G21230.1 75.926 54 13 0 2 55 359 412 6.82e-20 82.0
Msa0347960 AT2G21230.3 75.926 54 13 0 2 55 359 412 7.33e-20 82.0
Msa0347960 AT2G21230.2 75.926 54 13 0 2 55 359 412 2.53e-19 80.5
Msa0347960 AT4G38900.1 68.852 61 13 1 1 55 382 442 6.34e-19 79.3
Msa0347960 AT2G40620.1 70.909 55 16 0 1 55 136 190 1.58e-18 77.8
Msa0347960 AT2G31370.5 67.273 55 18 0 1 55 189 243 4.04e-17 73.9
Msa0347960 AT2G31370.2 67.273 55 18 0 1 55 189 243 4.75e-17 73.9
Msa0347960 AT2G31370.4 67.273 55 18 0 1 55 189 243 4.75e-17 73.9
Msa0347960 AT2G31370.1 67.273 55 18 0 1 55 189 243 4.75e-17 73.9
Msa0347960 AT2G31370.7 67.273 55 18 0 1 55 189 243 4.75e-17 73.9
Msa0347960 AT2G31370.3 67.273 55 18 0 1 55 189 243 4.75e-17 73.9
Msa0347960 AT2G31370.6 67.273 55 18 0 1 55 189 243 4.75e-17 73.9
Msa0347960 AT1G06070.1 70.588 51 15 0 5 55 202 252 5.69e-17 73.6
Msa0347960 AT1G06850.2 67.273 55 18 0 1 55 136 190 1.04e-16 71.6
Msa0347960 AT1G43700.1 63.636 55 20 0 1 55 182 236 1.84e-16 72.0
Msa0347960 AT1G06850.1 67.273 55 18 0 1 55 136 190 2.73e-16 71.6

Find 85 sgRNAs with CRISPR-Local

Find 164 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGGGTTATTTCAGGATTT+TGG 0.101232 3_1:+9716244 Msa0347960:intron
GGACCTTAGCAAACTGTATT+TGG 0.168656 3_1:+9717305 Msa0347960:three_prime_UTR
GCTTAAGATGCGATATATTT+TGG 0.170519 3_1:+9716298 Msa0347960:CDS
ATTTGGAAAATAAATTTATA+AGG 0.205498 3_1:+9717322 Msa0347960:three_prime_UTR
AATAAATTTATAAGGGGAAT+TGG 0.239221 3_1:+9717330 Msa0347960:three_prime_UTR
TTAACTTTGGCCACCTTTAC+GGG 0.250012 3_1:+9717020 Msa0347960:three_prime_UTR
GTTAACTTTGGCCACCTTTA+CGG 0.255562 3_1:+9717019 Msa0347960:three_prime_UTR
CGGTGAATCACCTACCTCTT+TGG 0.270020 3_1:+9715206 Msa0347960:five_prime_UTR
TGCTACCAACACTGCTTTCT+CGG 0.281836 3_1:-9716439 None:intergenic
GCAGTTGGGAAGATGTTTAT+TGG 0.300650 3_1:-9714961 None:intergenic
GGAAGATGTTTATTGGTAAC+AGG 0.305476 3_1:-9714954 None:intergenic
TACCCTATGCACAACCTTCT+AGG 0.309236 3_1:+9717058 Msa0347960:three_prime_UTR
TTTGGAAAATAAATTTATAA+GGG 0.316986 3_1:+9717323 Msa0347960:three_prime_UTR
GGATTGTAATCTTGAACTTC+AGG 0.321521 3_1:-9716611 None:intergenic
GTGATTCACCGAATCGAGTT+AGG 0.324969 3_1:-9715194 None:intergenic
TTCGGAGTTGAAGAAAATTA+TGG 0.328298 3_1:+9716096 Msa0347960:exon
AAATTGTTAAAGAGCTTTGT+AGG 0.344037 3_1:+9717284 Msa0347960:three_prime_UTR
AACACTGCTTTCTCGGTTCT+AGG 0.357024 3_1:-9716432 None:intergenic
GCAACGGCACAACGAATTCT+TGG 0.368486 3_1:-9716551 None:intergenic
GAGGTGTCATATTCTGAAAT+TGG 0.368658 3_1:-9716370 None:intergenic
ATAAATTTATAAGGGGAATT+GGG 0.372298 3_1:+9717331 Msa0347960:three_prime_UTR
ATCTAATGATTATAATCAAC+TGG 0.374871 3_1:-9717220 None:intergenic
TTTCTGAAATGCCTTCAGTT+TGG 0.388967 3_1:+9717422 Msa0347960:three_prime_UTR
GCATGGAAATTTGTTAACTT+TGG 0.389773 3_1:+9717007 Msa0347960:three_prime_UTR
AAAATAATACCTGGCCTTTG+TGG 0.394261 3_1:-9716650 None:intergenic
TTCATCGTGGTTTGTCTCAC+CGG 0.399368 3_1:+9715249 Msa0347960:five_prime_UTR
ACAATTCAATACAAAATGAA+TGG 0.430281 3_1:-9715001 None:intergenic
ATTGAAGGTCTTGTTGTGTT+TGG 0.431019 3_1:-9717123 None:intergenic
AATAGATGCAGTGCCCTAGA+AGG 0.445549 3_1:-9717072 None:intergenic
CTCAAAACCCTAACTCGATT+CGG 0.452533 3_1:+9715186 Msa0347960:five_prime_UTR
TTCAAGATTACAATCCCATA+TGG 0.457081 3_1:+9716618 Msa0347960:three_prime_UTR
CTATTCAGTTCCTTTGTGAA+TGG 0.458037 3_1:+9717090 Msa0347960:three_prime_UTR
TTGTGGAACCAAGCTCCATA+TGG 0.461206 3_1:-9716633 None:intergenic
TCAGTTCCTTTGTGAATGGC+AGG 0.462751 3_1:+9717094 Msa0347960:three_prime_UTR
ACAGCGAGTTTACCTTTGAC+AGG 0.463284 3_1:-9715294 None:intergenic
ATATCGCATCTTAAGCTCAA+TGG 0.469206 3_1:-9716291 None:intergenic
TGATTCACCGAATCGAGTTA+GGG 0.473094 3_1:-9715193 None:intergenic
CAGCACCTCTTAGCTCGCTT+CGG 0.474909 3_1:-9716154 None:intergenic
GAATTCGTTGTGCCGTTGCT+AGG 0.480369 3_1:+9716555 Msa0347960:three_prime_UTR
TTAGACTGAGAAGAATGCTT+CGG 0.481879 3_1:+9716078 Msa0347960:intron
GCCCTAGAAGGTTGTGCATA+GGG 0.481985 3_1:-9717060 None:intergenic
TGTGGAACCAAGCTCCATAT+GGG 0.483074 3_1:-9716632 None:intergenic
AAATTGTGTAAAGAACAATA+TGG 0.484537 3_1:-9717399 None:intergenic
GAAAGTTGCTGAGATTGTTA+TGG 0.484882 3_1:+9716126 Msa0347960:CDS
ACCCTATGCACAACCTTCTA+GGG 0.485272 3_1:+9717059 Msa0347960:three_prime_UTR
ACAACAAGACCTTCAATTGA+AGG 0.487972 3_1:+9717129 Msa0347960:three_prime_UTR
CTATGTGTAGGATGGAGGTT+TGG 0.488964 3_1:+9716975 Msa0347960:intron
GGAGGACGAAGATGCGAAAC+CGG 0.489146 3_1:-9715268 None:intergenic
TTTACGGGGAGCAGCTCATA+TGG 0.493055 3_1:+9717035 Msa0347960:three_prime_UTR
CAATTCAATACAAAATGAAT+GGG 0.495844 3_1:-9715000 None:intergenic
TTCCACATGTTATATACAGT+TGG 0.506432 3_1:-9717180 None:intergenic
ATCAGAGTCTCAATGCAGTT+GGG 0.512214 3_1:-9714975 None:intergenic
AATCAGAGTCTCAATGCAGT+TGG 0.516569 3_1:-9714976 None:intergenic
TTACAATCCCATATGGAGCT+TGG 0.521352 3_1:+9716625 Msa0347960:three_prime_UTR
TTTCCAAATACAGTTTGCTA+AGG 0.524653 3_1:-9717308 None:intergenic
GTTCAGACTCTGAAGACTGA+AGG 0.542615 3_1:+9716335 Msa0347960:CDS
TCGTCCTCCGTCCCTGTCAA+AGG 0.545355 3_1:+9715282 Msa0347960:five_prime_UTR
TTGGAAAATAAATTTATAAG+GGG 0.545493 3_1:+9717324 Msa0347960:three_prime_UTR
ATATGAGCTGCTCCCCGTAA+AGG 0.545844 3_1:-9717033 None:intergenic
AGGTTTGGCAACTTTGAGCA+TGG 0.546338 3_1:+9716990 Msa0347960:three_prime_UTR
TGCCCTAGAAGGTTGTGCAT+AGG 0.562054 3_1:-9717061 None:intergenic
CATATCTATGTGTAGGATGG+AGG 0.564335 3_1:+9716970 Msa0347960:intron
ATTACCTGTGAGGAGGAAAA+CGG 0.580405 3_1:-9715057 None:intergenic
AGCTTGGTTCCACAAAGGCC+AGG 0.580658 3_1:+9716641 Msa0347960:three_prime_UTR
CTGATCCGAAGCGAGCTAAG+AGG 0.581733 3_1:+9716149 Msa0347960:CDS
TTCAGACTCTGAAGACTGAA+GGG 0.583079 3_1:+9716336 Msa0347960:CDS
CAGCGAGTTTACCTTTGACA+GGG 0.588627 3_1:-9715293 None:intergenic
TGCCAACTGTATATAACATG+TGG 0.599624 3_1:+9717178 Msa0347960:three_prime_UTR
TGAAGCAAACTCAGCAAGAG+AGG 0.601941 3_1:-9716389 None:intergenic
CGAAAACTGATCCAAACTGA+AGG 0.608245 3_1:-9717433 None:intergenic
GAGTTTACCTTTGACAGGGA+CGG 0.609469 3_1:-9715289 None:intergenic
CGCCGAGATTACCTGTGAGG+AGG 0.613925 3_1:-9715064 None:intergenic
TAACTTTGGCCACCTTTACG+GGG 0.615462 3_1:+9717021 Msa0347960:three_prime_UTR
TTTACCTTTGACAGGGACGG+AGG 0.619960 3_1:-9715286 None:intergenic
CAATCTCCTGCCATTCACAA+AGG 0.622463 3_1:-9717100 None:intergenic
TGAGCTGCTCCCCGTAAAGG+TGG 0.627202 3_1:-9717030 None:intergenic
TCAGAGCAAACCAAAGAGGT+AGG 0.627547 3_1:-9715216 None:intergenic
GTGAGACAAACCACGATGAA+CGG 0.631636 3_1:-9715246 None:intergenic
CTACATTGGTAACATACACA+CGG 0.650580 3_1:+9714929 None:intergenic
TGATCGATCACCGTTCATCG+TGG 0.658192 3_1:+9715236 Msa0347960:five_prime_UTR
TCGATCAGAGCAAACCAAAG+AGG 0.661369 3_1:-9715220 None:intergenic
TATGGAGCTTGGTTCCACAA+AGG 0.680414 3_1:+9716636 Msa0347960:three_prime_UTR
TGGCGCCGAGATTACCTGTG+AGG 0.706032 3_1:-9715067 None:intergenic
AGTGCTCGAGAACCTAGCAA+CGG 0.707214 3_1:-9716567 None:intergenic
ACCTGTGAGGAGGAAAACGG+TGG 0.736957 3_1:-9715054 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTGGAAAATAAATTTATA+AGG + chr3_1:9717322-9717341 Msa0347960:three_prime_UTR 10.0%
!! TTTGGAAAATAAATTTATAA+GGG + chr3_1:9717323-9717342 Msa0347960:three_prime_UTR 10.0%
!! ATCATAAAAAATAAAATGAA+TGG - chr3_1:9717370-9717389 None:intergenic 10.0%
!! AAAAACATTTAAACTTCATA+AGG - chr3_1:9715792-9715811 None:intergenic 15.0%
!!! ATTTAACTTGGATATTTTTT+TGG + chr3_1:9716226-9716245 Msa0347960:intron 15.0%
!!! TTTAACTTGGATATTTTTTT+GGG + chr3_1:9716227-9716246 Msa0347960:intron 15.0%
!! AGAAATGATCAATAAAAATT+TGG - chr3_1:9716471-9716490 None:intergenic 15.0%
!! TTGGAAAATAAATTTATAAG+GGG + chr3_1:9717324-9717343 Msa0347960:three_prime_UTR 15.0%
!! CAATTCAATACAAAATGAAT+GGG - chr3_1:9715003-9715022 None:intergenic 20.0%
!! ACAATTCAATACAAAATGAA+TGG - chr3_1:9715004-9715023 None:intergenic 20.0%
!!! ATAACTAATTTTCATTTTGC+TGG + chr3_1:9715594-9715613 Msa0347960:intron 20.0%
!!! CATCATTAGTTTTATGATTT+TGG + chr3_1:9715678-9715697 Msa0347960:intron 20.0%
!!! TTGTGATTTATGTGTATTTT+AGG + chr3_1:9715810-9715829 Msa0347960:intron 20.0%
!!! TTTATGTGTATTTTAGGATA+AGG + chr3_1:9715816-9715835 Msa0347960:intron 20.0%
!! TTTAGTTAGTAATGTCAATA+TGG + chr3_1:9716196-9716215 Msa0347960:intron 20.0%
!!! ATATTTTTTTGGGTTATTTC+AGG + chr3_1:9716237-9716256 Msa0347960:intron 20.0%
!! GTGTTAAATTATTATGACAA+CGG + chr3_1:9716525-9716544 Msa0347960:intron 20.0%
!! CTGTTAAAAAAAATAATACC+TGG - chr3_1:9716662-9716681 None:intergenic 20.0%
!! TAACTTAGTATTCTATAGTA+TGG + chr3_1:9716709-9716728 Msa0347960:intron 20.0%
!! ATTGAAGGATGAAAAAAAAA+TGG + chr3_1:9717144-9717163 Msa0347960:three_prime_UTR 20.0%
!! TTGAAGGATGAAAAAAAAAT+GGG + chr3_1:9717145-9717164 Msa0347960:three_prime_UTR 20.0%
!! ATCTAATGATTATAATCAAC+TGG - chr3_1:9717223-9717242 None:intergenic 20.0%
!! AATAAATTTATAAGGGGAAT+TGG + chr3_1:9717330-9717349 Msa0347960:three_prime_UTR 20.0%
!! ATAAATTTATAAGGGGAATT+GGG + chr3_1:9717331-9717350 Msa0347960:three_prime_UTR 20.0%
!! AAATTGTGTAAAGAACAATA+TGG - chr3_1:9717402-9717421 None:intergenic 20.0%
!!! ATTTTAAGAATGTTGTTGAT+TGG - chr3_1:9717466-9717485 None:intergenic 20.0%
! TGACAGTAGACAAAAAAATT+AGG - chr3_1:9715435-9715454 None:intergenic 25.0%
!!! TTTACTGCTCTTATTTTACT+TGG + chr3_1:9715949-9715968 Msa0347960:intron 25.0%
! CATCAAAATTAGTTCAAATC+AGG - chr3_1:9715992-9716011 None:intergenic 25.0%
!!! TTGAACTAATTTTGATGTCT+TGG + chr3_1:9715995-9716014 Msa0347960:intron 25.0%
! TATGGATGGTTAATTTAACT+TGG + chr3_1:9716214-9716233 Msa0347960:intron 25.0%
!!! ATTTTTCGAAATGCAGAAAA+TGG - chr3_1:9716690-9716709 None:intergenic 25.0%
!!! TTTTTTCATCCTTCAATTGA+AGG - chr3_1:9717141-9717160 None:intergenic 25.0%
!! AAATTGTTAAAGAGCTTTGT+AGG + chr3_1:9717284-9717303 Msa0347960:three_prime_UTR 25.0%
!! CATAAGGTACTTTTCTAACA+TGG - chr3_1:9715776-9715795 None:intergenic 30.0%
ACAATGTGTGCTTACAATAT+GGG + chr3_1:9715886-9715905 Msa0347960:intron 30.0%
TTCGGAGTTGAAGAAAATTA+TGG + chr3_1:9716096-9716115 Msa0347960:exon 30.0%
GTTAGTAATGTCAATATGGA+TGG + chr3_1:9716200-9716219 Msa0347960:intron 30.0%
!! TTTGGGTTATTTCAGGATTT+TGG + chr3_1:9716244-9716263 Msa0347960:intron 30.0%
! GCTTAAGATGCGATATATTT+TGG + chr3_1:9716298-9716317 Msa0347960:CDS 30.0%
! ATTAGCTTCTTAGTAATCAG+AGG - chr3_1:9716502-9716521 None:intergenic 30.0%
TTCAAGATTACAATCCCATA+TGG + chr3_1:9716618-9716637 Msa0347960:three_prime_UTR 30.0%
TTGTCACTGTTGTCAAATAA+AGG + chr3_1:9716776-9716795 Msa0347960:intron 30.0%
TTGTCAAATAAAGGCTATAG+TGG + chr3_1:9716785-9716804 Msa0347960:intron 30.0%
!! GCATGGAAATTTGTTAACTT+TGG + chr3_1:9717007-9717026 Msa0347960:three_prime_UTR 30.0%
TTCCACATGTTATATACAGT+TGG - chr3_1:9717183-9717202 None:intergenic 30.0%
!! TTTCCAAATACAGTTTGCTA+AGG - chr3_1:9717311-9717330 None:intergenic 30.0%
! GGAAGATGTTTATTGGTAAC+AGG - chr3_1:9714957-9714976 None:intergenic 35.0%
TCGAATGGAGCTAAAATTGA+AGG - chr3_1:9715160-9715179 None:intergenic 35.0%
!!! GGTTTTGAGAGAAAATCGAA+TGG - chr3_1:9715175-9715194 None:intergenic 35.0%
AAGGTGAAAGAAAGAAAGCA+AGG - chr3_1:9715327-9715346 None:intergenic 35.0%
TTGCTTTCTTTCTTTCACCT+TGG + chr3_1:9715326-9715345 Msa0347960:intron 35.0%
! GCTTTTCGTGTTGAAAAGAA+TGG + chr3_1:9715348-9715367 Msa0347960:intron 35.0%
TGTCACAACATGTCTTTCTT+AGG + chr3_1:9715450-9715469 Msa0347960:intron 35.0%
! AGGTACTTTTCTAACATGGT+AGG - chr3_1:9715772-9715791 None:intergenic 35.0%
GACAATGTGTGCTTACAATA+TGG + chr3_1:9715885-9715904 Msa0347960:intron 35.0%
TTAGACTGAGAAGAATGCTT+CGG + chr3_1:9716078-9716097 Msa0347960:intron 35.0%
GAAAGTTGCTGAGATTGTTA+TGG + chr3_1:9716126-9716145 Msa0347960:CDS 35.0%
!!! ATTTCAGGATTTTGGCTAAC+AGG + chr3_1:9716252-9716271 Msa0347960:intron 35.0%
ATATCGCATCTTAAGCTCAA+TGG - chr3_1:9716294-9716313 None:intergenic 35.0%
!!! TATTTTGGAGCTAGAACACA+AGG + chr3_1:9716313-9716332 Msa0347960:CDS 35.0%
!! GAGGTGTCATATTCTGAAAT+TGG - chr3_1:9716373-9716392 None:intergenic 35.0%
GGATTGTAATCTTGAACTTC+AGG - chr3_1:9716614-9716633 None:intergenic 35.0%
! AAAATAATACCTGGCCTTTG+TGG - chr3_1:9716653-9716672 None:intergenic 35.0%
TGCATTGCATATCTATGTGT+AGG + chr3_1:9716963-9716982 Msa0347960:intron 35.0%
TTGCATATCTATGTGTAGGA+TGG + chr3_1:9716967-9716986 Msa0347960:intron 35.0%
CTATTCAGTTCCTTTGTGAA+TGG + chr3_1:9717090-9717109 Msa0347960:three_prime_UTR 35.0%
!! ATTGAAGGTCTTGTTGTGTT+TGG - chr3_1:9717126-9717145 None:intergenic 35.0%
ACAACAAGACCTTCAATTGA+AGG + chr3_1:9717129-9717148 Msa0347960:three_prime_UTR 35.0%
TGCCAACTGTATATAACATG+TGG + chr3_1:9717178-9717197 Msa0347960:three_prime_UTR 35.0%
!! TTTCTGAAATGCCTTCAGTT+TGG + chr3_1:9717422-9717441 Msa0347960:three_prime_UTR 35.0%
GCAGTTGGGAAGATGTTTAT+TGG - chr3_1:9714964-9714983 None:intergenic 40.0%
ATCAGAGTCTCAATGCAGTT+GGG - chr3_1:9714978-9714997 None:intergenic 40.0%
AATCAGAGTCTCAATGCAGT+TGG - chr3_1:9714979-9714998 None:intergenic 40.0%
ATTACCTGTGAGGAGGAAAA+CGG - chr3_1:9715060-9715079 None:intergenic 40.0%
CTCAAAACCCTAACTCGATT+CGG + chr3_1:9715186-9715205 Msa0347960:five_prime_UTR 40.0%
TGATTCACCGAATCGAGTTA+GGG - chr3_1:9715196-9715215 None:intergenic 40.0%
! CTTTTCAACACGAAAAGCCA+AGG - chr3_1:9715346-9715365 None:intergenic 40.0%
! ACCTTGTTATGCTTGTGTAG+GGG - chr3_1:9715510-9715529 None:intergenic 40.0%
! CACCTTGTTATGCTTGTGTA+GGG - chr3_1:9715511-9715530 None:intergenic 40.0%
CTACACAAGCATAACAAGGT+GGG + chr3_1:9715510-9715529 Msa0347960:intron 40.0%
GCTTAACTAATTGTGCAGGT+TGG - chr3_1:9715748-9715767 None:intergenic 40.0%
AGGAGCTTAACTAATTGTGC+AGG - chr3_1:9715752-9715771 None:intergenic 40.0%
TAAGGCTCCATTCATTGCTA+AGG + chr3_1:9715834-9715853 Msa0347960:intron 40.0%
!!! GATTTTGGCTAACAGGCAAT+CGG + chr3_1:9716259-9716278 Msa0347960:CDS 40.0%
! TTCAGACTCTGAAGACTGAA+GGG + chr3_1:9716336-9716355 Msa0347960:CDS 40.0%
TTACAATCCCATATGGAGCT+TGG + chr3_1:9716625-9716644 Msa0347960:three_prime_UTR 40.0%
! GACAAAAACCACTGCTGATT+AGG - chr3_1:9716761-9716780 None:intergenic 40.0%
!! ACCATTATTTCTGCGATTCG+TGG + chr3_1:9716902-9716921 Msa0347960:intron 40.0%
ACCACGAATCGCAGAAATAA+TGG - chr3_1:9716906-9716925 None:intergenic 40.0%
CATATCTATGTGTAGGATGG+AGG + chr3_1:9716970-9716989 Msa0347960:intron 40.0%
GTTAACTTTGGCCACCTTTA+CGG + chr3_1:9717019-9717038 Msa0347960:three_prime_UTR 40.0%
TTAACTTTGGCCACCTTTAC+GGG + chr3_1:9717020-9717039 Msa0347960:three_prime_UTR 40.0%
GGACCTTAGCAAACTGTATT+TGG + chr3_1:9717305-9717324 Msa0347960:three_prime_UTR 40.0%
CGAAAACTGATCCAAACTGA+AGG - chr3_1:9717436-9717455 None:intergenic 40.0%
TTCCTCCTCACAGGTAATCT+CGG + chr3_1:9715062-9715081 Msa0347960:intron 45.0%
GAAGAAGGAGGTGATGCTAT+CGG - chr3_1:9715100-9715119 None:intergenic 45.0%
ATAGCATCACCTCCTTCTTC+CGG + chr3_1:9715100-9715119 Msa0347960:intron 45.0%
ATAGATGCATATGACGTCGC+CGG - chr3_1:9715122-9715141 None:intergenic 45.0%
GTGATTCACCGAATCGAGTT+AGG - chr3_1:9715197-9715216 None:intergenic 45.0%
TCAGAGCAAACCAAAGAGGT+AGG - chr3_1:9715219-9715238 None:intergenic 45.0%
TCGATCAGAGCAAACCAAAG+AGG - chr3_1:9715223-9715242 None:intergenic 45.0%
GTGAGACAAACCACGATGAA+CGG - chr3_1:9715249-9715268 None:intergenic 45.0%
! TTCATCGTGGTTTGTCTCAC+CGG + chr3_1:9715249-9715268 Msa0347960:five_prime_UTR 45.0%
GAGTTTACCTTTGACAGGGA+CGG - chr3_1:9715292-9715311 None:intergenic 45.0%
CAGCGAGTTTACCTTTGACA+GGG - chr3_1:9715296-9715315 None:intergenic 45.0%
ACAGCGAGTTTACCTTTGAC+AGG - chr3_1:9715297-9715316 None:intergenic 45.0%
TCTTAGGCTGCTGAGAATGA+TGG + chr3_1:9715466-9715485 Msa0347960:intron 45.0%
! CCACCTTGTTATGCTTGTGT+AGG - chr3_1:9715512-9715531 None:intergenic 45.0%
CCTACACAAGCATAACAAGG+TGG + chr3_1:9715509-9715528 Msa0347960:intron 45.0%
TGCTGGCAGATCGAAACTTA+TGG + chr3_1:9715611-9715630 Msa0347960:intron 45.0%
CTCCATTCATTGCTAAGGCT+GGG + chr3_1:9715839-9715858 Msa0347960:intron 45.0%
TTCCCAGCCTTAGCAATGAA+TGG - chr3_1:9715844-9715863 None:intergenic 45.0%
TTGGACTGAGAAGACTGCTT+CGG + chr3_1:9716014-9716033 Msa0347960:intron 45.0%
! GTTCAGACTCTGAAGACTGA+AGG + chr3_1:9716335-9716354 Msa0347960:CDS 45.0%
TGAAGCAAACTCAGCAAGAG+AGG - chr3_1:9716392-9716411 None:intergenic 45.0%
AACACTGCTTTCTCGGTTCT+AGG - chr3_1:9716435-9716454 None:intergenic 45.0%
TAGAACCGAGAAAGCAGTGT+TGG + chr3_1:9716434-9716453 Msa0347960:intron 45.0%
TGCTACCAACACTGCTTTCT+CGG - chr3_1:9716442-9716461 None:intergenic 45.0%
TGTGGAACCAAGCTCCATAT+GGG - chr3_1:9716635-9716654 None:intergenic 45.0%
TTGTGGAACCAAGCTCCATA+TGG - chr3_1:9716636-9716655 None:intergenic 45.0%
TATGGAGCTTGGTTCCACAA+AGG + chr3_1:9716636-9716655 Msa0347960:three_prime_UTR 45.0%
! CCACTGCTGATTAGGAAAAC+TGG - chr3_1:9716753-9716772 None:intergenic 45.0%
! CCAGTTTTCCTAATCAGCAG+TGG + chr3_1:9716750-9716769 Msa0347960:intron 45.0%
CTATGTGTAGGATGGAGGTT+TGG + chr3_1:9716975-9716994 Msa0347960:intron 45.0%
AGGTTTGGCAACTTTGAGCA+TGG + chr3_1:9716990-9717009 Msa0347960:three_prime_UTR 45.0%
TAACTTTGGCCACCTTTACG+GGG + chr3_1:9717021-9717040 Msa0347960:three_prime_UTR 45.0%
TACCCTATGCACAACCTTCT+AGG + chr3_1:9717058-9717077 Msa0347960:three_prime_UTR 45.0%
ACCCTATGCACAACCTTCTA+GGG + chr3_1:9717059-9717078 Msa0347960:three_prime_UTR 45.0%
AATAGATGCAGTGCCCTAGA+AGG - chr3_1:9717075-9717094 None:intergenic 45.0%
TCAGTTCCTTTGTGAATGGC+AGG + chr3_1:9717094-9717113 Msa0347960:three_prime_UTR 45.0%
CAATCTCCTGCCATTCACAA+AGG - chr3_1:9717103-9717122 None:intergenic 45.0%
GTGATGCTATCGGAGGAAGA+TGG - chr3_1:9715090-9715109 None:intergenic 50.0%
CGGTGAATCACCTACCTCTT+TGG + chr3_1:9715206-9715225 Msa0347960:five_prime_UTR 50.0%
TGATCGATCACCGTTCATCG+TGG + chr3_1:9715236-9715255 Msa0347960:five_prime_UTR 50.0%
TTTACCTTTGACAGGGACGG+AGG - chr3_1:9715289-9715308 None:intergenic 50.0%
GCCCCTACACAAGCATAACA+AGG + chr3_1:9715506-9715525 Msa0347960:intron 50.0%
GCTCCATTCATTGCTAAGGC+TGG + chr3_1:9715838-9715857 Msa0347960:intron 50.0%
TGCTAAGGCTGGGAAGTTGA+AGG + chr3_1:9715849-9715868 Msa0347960:intron 50.0%
TAAGGCTGGGAAGTTGAAGG+AGG + chr3_1:9715852-9715871 Msa0347960:intron 50.0%
AAGACTGCTTCGGAGGAAGA+AGG + chr3_1:9716024-9716043 Msa0347960:intron 50.0%
GCAACGGCACAACGAATTCT+TGG - chr3_1:9716554-9716573 None:intergenic 50.0%
! GAATTCGTTGTGCCGTTGCT+AGG + chr3_1:9716555-9716574 Msa0347960:three_prime_UTR 50.0%
AGTGCTCGAGAACCTAGCAA+CGG - chr3_1:9716570-9716589 None:intergenic 50.0%
TATAGTGGCGCTTTGCGTAG+TGG + chr3_1:9716800-9716819 Msa0347960:intron 50.0%
CGATTCGTGGTTGACATCAC+TGG + chr3_1:9716915-9716934 Msa0347960:intron 50.0%
ATATGAGCTGCTCCCCGTAA+AGG - chr3_1:9717036-9717055 None:intergenic 50.0%
TTTACGGGGAGCAGCTCATA+TGG + chr3_1:9717035-9717054 Msa0347960:three_prime_UTR 50.0%
GCCCTAGAAGGTTGTGCATA+GGG - chr3_1:9717063-9717082 None:intergenic 50.0%
TGCCCTAGAAGGTTGTGCAT+AGG - chr3_1:9717064-9717083 None:intergenic 50.0%
! ACCACCGTTTTCCTCCTCAC+AGG + chr3_1:9715053-9715072 Msa0347960:five_prime_UTR 55.0%
ACCTGTGAGGAGGAAAACGG+TGG - chr3_1:9715057-9715076 None:intergenic 55.0%
GAAGGAGGTGATGCTATCGG+AGG - chr3_1:9715097-9715116 None:intergenic 55.0%
CATATGACGTCGCCGGAAGA+AGG - chr3_1:9715115-9715134 None:intergenic 55.0%
GGAGGACGAAGATGCGAAAC+CGG - chr3_1:9715271-9715290 None:intergenic 55.0%
GACTGAGAAGACTGCTTCGG+AGG + chr3_1:9716017-9716036 Msa0347960:intron 55.0%
CTGATCCGAAGCGAGCTAAG+AGG + chr3_1:9716149-9716168 Msa0347960:CDS 55.0%
CAGCACCTCTTAGCTCGCTT+CGG - chr3_1:9716157-9716176 None:intergenic 55.0%
AGCTTGGTTCCACAAAGGCC+AGG + chr3_1:9716641-9716660 Msa0347960:three_prime_UTR 55.0%
CGCCGAGATTACCTGTGAGG+AGG - chr3_1:9715067-9715086 None:intergenic 60.0%
! TGGCGCCGAGATTACCTGTG+AGG - chr3_1:9715070-9715089 None:intergenic 60.0%
ATGACGTCGCCGGAAGAAGG+AGG - chr3_1:9715112-9715131 None:intergenic 60.0%
TCGTCCTCCGTCCCTGTCAA+AGG + chr3_1:9715282-9715301 Msa0347960:five_prime_UTR 60.0%
TGAGCTGCTCCCCGTAAAGG+TGG - chr3_1:9717033-9717052 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 9714932 9717519 9714932 ID=Msa0347960;Name=Msa0347960
chr3_1 mRNA 9714932 9717519 9714932 ID=Msa0347960-mRNA-1;Parent=Msa0347960;Name=Msa0347960-mRNA-1;_AED=0.11;_eAED=0.11;_QI=310|0.8|0.83|1|0.4|0.33|6|668|77
chr3_1 exon 9714932 9715074 9714932 ID=Msa0347960-mRNA-1:exon:2720;Parent=Msa0347960-mRNA-1
chr3_1 exon 9715170 9715303 9715170 ID=Msa0347960-mRNA-1:exon:2721;Parent=Msa0347960-mRNA-1
chr3_1 exon 9716082 9716170 9716082 ID=Msa0347960-mRNA-1:exon:2722;Parent=Msa0347960-mRNA-1
chr3_1 exon 9716259 9716451 9716259 ID=Msa0347960-mRNA-1:exon:2723;Parent=Msa0347960-mRNA-1
chr3_1 exon 9716545 9716662 9716545 ID=Msa0347960-mRNA-1:exon:2724;Parent=Msa0347960-mRNA-1
chr3_1 exon 9716985 9717519 9716985 ID=Msa0347960-mRNA-1:exon:2725;Parent=Msa0347960-mRNA-1
chr3_1 five_prime_UTR 9714932 9715074 9714932 ID=Msa0347960-mRNA-1:five_prime_utr;Parent=Msa0347960-mRNA-1
chr3_1 five_prime_UTR 9715170 9715303 9715170 ID=Msa0347960-mRNA-1:five_prime_utr;Parent=Msa0347960-mRNA-1
chr3_1 five_prime_UTR 9716082 9716114 9716082 ID=Msa0347960-mRNA-1:five_prime_utr;Parent=Msa0347960-mRNA-1
chr3_1 CDS 9716115 9716170 9716115 ID=Msa0347960-mRNA-1:cds;Parent=Msa0347960-mRNA-1
chr3_1 CDS 9716259 9716436 9716259 ID=Msa0347960-mRNA-1:cds;Parent=Msa0347960-mRNA-1
chr3_1 three_prime_UTR 9716437 9716451 9716437 ID=Msa0347960-mRNA-1:three_prime_utr;Parent=Msa0347960-mRNA-1
chr3_1 three_prime_UTR 9716545 9716662 9716545 ID=Msa0347960-mRNA-1:three_prime_utr;Parent=Msa0347960-mRNA-1
chr3_1 three_prime_UTR 9716985 9717519 9716985 ID=Msa0347960-mRNA-1:three_prime_utr;Parent=Msa0347960-mRNA-1
Gene Sequence

>Msa0347960

ATGGCTAATGAGAAAGTTGCTGAGATTGTTATGGCTGATCCGAAGCGAGCTAAGAGGATTTTGGCTAACAGGCAATCGGCTGCGCGTTCCATTGAGCTTAAGATGCGATATATTTTGGAGCTAGAACACAAGGTTCAGACTCTGAAGACTGAAGGGTTGTTTCTTCTCCAATTTCAGAATATGACACCTCTCTTGCTGAGTTTGCTTCAACAATTGTTGAGTTATGAAGCCTAG
Protein sequence

>Msa0347960

MANEKVAEIVMADPKRAKRILANRQSAARSIELKMRYILELEHKVQTLKTEGLFLLQFQNMTPLLLSLLQQLLSYEA*