AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0348030

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0348030 MtrunA17_Chr3g0080401 80.119 337 49 6 81 401 1 335 0.0 540
Msa0348030 MtrunA17_Chr3g0118361 53.357 283 118 3 32 300 51 333 1.82e-104 326
Msa0348030 MtrunA17_Chr5g0440591 54.406 261 113 1 29 283 45 305 2.08e-102 320
Msa0348030 MtrunA17_Chr6g0476301 47.761 335 141 6 81 388 1 328 1.03e-95 295
Msa0348030 MtrunA17_Chr2g0329151 53.114 273 112 5 17 279 24 290 1.22e-94 300
Msa0348030 MtrunA17_Chr6g0473191 59.664 238 83 3 81 305 1 238 6.27e-94 288
Msa0348030 MtrunA17_Chr6g0476291 57.563 238 88 3 81 305 1 238 1.04e-90 278
Msa0348030 MtrunA17_Chr3g0124291 48.594 249 123 3 33 276 32 280 3.54e-84 267
Msa0348030 MtrunA17_Chr8g0382561 49.805 257 121 4 26 276 17 271 1.15e-80 258
Msa0348030 MtrunA17_Chr7g0260281 36.667 120 69 2 70 182 1 120 1.53e-15 73.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0348030 AT2G27050.1 54.307 267 108 3 29 284 45 308 2.68e-103 322
Msa0348030 AT3G20770.1 52.098 286 129 2 5 284 23 306 1.32e-101 318
Msa0348030 AT1G73730.2 47.222 324 152 6 16 331 11 323 1.38e-94 298
Msa0348030 AT1G73730.1 47.222 324 152 6 16 331 23 335 1.65e-94 298
Msa0348030 AT5G10120.1 45.977 261 130 4 26 276 16 275 6.73e-80 258
Msa0348030 AT5G65100.1 42.446 278 134 5 25 278 32 307 6.22e-74 244
Msa0348030 AT5G21120.3 43.493 292 130 8 38 297 53 341 1.60e-72 239
Msa0348030 AT5G21120.1 43.493 292 130 8 38 297 53 341 1.60e-72 239
Msa0348030 AT5G21120.2 43.493 292 130 8 38 297 60 348 1.79e-72 239

Find 106 sgRNAs with CRISPR-Local

Find 185 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCACATTGTTCTTCATATT+TGG 0.130679 3_1:-9826242 None:intergenic
TTCTCTTGGCCAAACAATAT+TGG 0.211643 3_1:+9827828 Msa0348030:CDS
TGTTGCTGCTTCCTCCTATT+TGG 0.243104 3_1:-9826945 None:intergenic
AGATTTGAGGCTGAAAATTT+TGG 0.272574 3_1:-9827506 None:intergenic
ATGCTAAGGACACAGCTATT+TGG 0.278587 3_1:+9826626 Msa0348030:CDS
CTCCTGATCTTCTGAAAATT+AGG 0.282984 3_1:-9826565 None:intergenic
CCATTTGAGGCTGCATGTTT+TGG 0.285639 3_1:-9827590 None:intergenic
TCTCTTGGCCAAACAATATT+GGG 0.290198 3_1:+9827829 Msa0348030:CDS
CACATGATTTGAAGAAGGTT+TGG 0.299071 3_1:+9826503 Msa0348030:CDS
TAACTTTGTGTTGCCACATT+AGG 0.315473 3_1:-9827801 None:intergenic
CAACTCCAACCATTTGGAAT+TGG 0.333568 3_1:-9826972 None:intergenic
TTCATCTTTAGATGAGTTGA+TGG 0.334799 3_1:+9827731 Msa0348030:CDS
TATTAATTCCCAATATTGTT+TGG 0.336531 3_1:-9827837 None:intergenic
ACAAACTCATAACTACTTTC+AGG 0.343492 3_1:-9826694 None:intergenic
GATGTTCGTGGCTTCGTCTA+TGG 0.352458 3_1:+9826115 Msa0348030:CDS
AGCTTCCAACTCCAACCATT+TGG 0.359268 3_1:-9826978 None:intergenic
CAAACCAATGATTGGCTCTT+CGG 0.371062 3_1:+9826156 Msa0348030:CDS
ATTGAACCTAATATTGTATC+AGG 0.373081 3_1:-9826328 None:intergenic
ATCATTGGTTTGCCATTGTC+AGG 0.373218 3_1:-9826145 None:intergenic
CGAATCATGGTGGAAGGAAA+TGG 0.374307 3_1:+9826441 Msa0348030:CDS
CCTCCACCATGGTGGCCAAC+AGG 0.377036 3_1:+9826415 Msa0348030:CDS
AAGAGAAAGGCTCACCAAAT+AGG 0.383738 3_1:+9826931 Msa0348030:CDS
GACCTACCTGATACAATATT+AGG 0.387617 3_1:+9826322 Msa0348030:CDS
TCATATTTGGCGATTGCAGC+TGG 0.389765 3_1:-9826229 None:intergenic
CACAAAGTTATGATTTCTCT+TGG 0.396627 3_1:+9827814 Msa0348030:CDS
GTTGGAATCATGTTGGTAAC+TGG 0.406571 3_1:-9827555 None:intergenic
TAGAAATTCTGCTCCATTTG+AGG 0.408160 3_1:-9827603 None:intergenic
TGAAAGTAGTTATGAGTTTG+TGG 0.410008 3_1:+9826696 Msa0348030:CDS
GTGCACAAGAGGGCATATTA+AGG 0.410074 3_1:+9825006 Msa0348030:CDS
ATCATTGAAGTGTAGATTTG+AGG 0.410822 3_1:-9827519 None:intergenic
CCAAAACATGCAGCCTCAAA+TGG 0.411657 3_1:+9827590 Msa0348030:CDS
CCCTCATCACATGATGATAA+AGG 0.413199 3_1:-9826766 None:intergenic
TATTAGTTATGAAGAAAGAT+TGG 0.415453 3_1:+9826657 Msa0348030:CDS
ACCCCAAAGAAAACATCCTT+TGG 0.424688 3_1:+9826381 Msa0348030:CDS
CGGAAAATCTACGCGGTTGG+TGG 0.429144 3_1:+9826176 Msa0348030:CDS
CAATCTTGTTATTGAGAGTA+GGG 0.434145 3_1:+9826900 Msa0348030:CDS
GGAGTATTGTTAGACATTCC+AGG 0.438919 3_1:+9826584 Msa0348030:CDS
AGGAACTAGAGACAAAGATA+TGG 0.443648 3_1:+9824889 Msa0348030:CDS
GACAATGGCTCGTGCACAAG+AGG 0.447975 3_1:+9824995 Msa0348030:CDS
GAAGGAAATGGGATTTAGTA+AGG 0.462346 3_1:+9826453 Msa0348030:CDS
CACCATGATTCGTTCCCTGT+TGG 0.467429 3_1:-9826430 None:intergenic
GACAATGGCAAACCAATGAT+TGG 0.467907 3_1:+9826148 Msa0348030:CDS
AAAACCACATGATTTGAAGA+AGG 0.473630 3_1:+9826498 Msa0348030:CDS
TGTTGCTTGATGAGCAAGTA+AGG 0.474666 3_1:+9826284 Msa0348030:CDS
CAATTCCAAATGGTTGGAGT+TGG 0.477252 3_1:+9826973 Msa0348030:CDS
GAAGCACCGTTATCAACGTT+GGG 0.485553 3_1:-9824798 None:intergenic
GGCCAACAGGGAACGAATCA+TGG 0.488109 3_1:+9826428 Msa0348030:CDS
ACCAACATGATTCCAACTCC+TGG 0.488765 3_1:+9827561 Msa0348030:CDS
GCAAAATAAATTCAATGCTA+AGG 0.497897 3_1:+9826612 Msa0348030:CDS
TGTGAAGTTTCAACAGTGAA+TGG 0.499106 3_1:+9826259 Msa0348030:CDS
CCTGTTGGCCACCATGGTGG+AGG 0.500513 3_1:-9826415 None:intergenic
AAAGTAAACTTTGAGGAAAA+CGG 0.500540 3_1:+9826205 Msa0348030:CDS
ACATTAACGACTTCAGCATT+AGG 0.506244 3_1:-9827669 None:intergenic
GGTAGGTCATACAAGGAGTA+AGG 0.514149 3_1:-9826307 None:intergenic
AACATGATTCCTACTCCTGC+TGG 0.514537 3_1:+9827477 Msa0348030:CDS
GAATCATGGTGGAAGGAAAT+GGG 0.515364 3_1:+9826442 Msa0348030:CDS
AACAATGCAAAACAAGATAA+CGG 0.516750 3_1:+9824822 Msa0348030:CDS
TTGGAAAATTTGTGTGTTGA+CGG 0.518161 3_1:+9826522 Msa0348030:CDS
AACAACCAATTCCAAATGGT+TGG 0.519990 3_1:+9826967 Msa0348030:CDS
AAATCTACGCGGTTGGTGGA+AGG 0.522592 3_1:+9826180 Msa0348030:CDS
AAGTGACAATAAAACATCAC+TGG 0.527333 3_1:+9824956 Msa0348030:CDS
CAAACCTTCTTCAAATCATG+TGG 0.527454 3_1:-9826502 None:intergenic
CAACAACAACCAATTCCAAA+TGG 0.529692 3_1:+9826963 Msa0348030:CDS
AACCTCCATGATGATTGTCT+TGG 0.533086 3_1:-9826867 None:intergenic
ACCTTTATCATCATGTGATG+AGG 0.538023 3_1:+9826765 Msa0348030:CDS
ACAATCTTGTTATTGAGAGT+AGG 0.541787 3_1:+9826899 Msa0348030:CDS
CCTTTATCATCATGTGATGA+GGG 0.544805 3_1:+9826766 Msa0348030:CDS
ATGCATCCCAACGTTGATAA+CGG 0.547251 3_1:+9824792 Msa0348030:CDS
CAGAATTTCTATGATCAACA+AGG 0.550654 3_1:+9827615 Msa0348030:CDS
TAGGGGTGCTAATAAGAGAA+AGG 0.554483 3_1:+9826918 Msa0348030:CDS
TGAAGCACCGTTATCAACGT+TGG 0.555762 3_1:-9824799 None:intergenic
CTCATCTAAAGATGAATCGA+AGG 0.557404 3_1:-9827724 None:intergenic
CTTCGGAAAATCTACGCGGT+TGG 0.564527 3_1:+9826173 Msa0348030:CDS
TGGTAATCACCAGCAGGAGT+AGG 0.564794 3_1:-9827486 None:intergenic
AAACATAGATATGACTATTG+AGG 0.565786 3_1:+9824869 Msa0348030:CDS
ACTTATTGATTCTCCTAATG+TGG 0.567478 3_1:+9827788 Msa0348030:CDS
TCGTTCCCTGTTGGCCACCA+TGG 0.567749 3_1:-9826421 None:intergenic
CAGGTACATGTTAAAGATGG+TGG 0.568652 3_1:+9826084 Msa0348030:intron
TGATGATTGTCTTGGTAATG+TGG 0.571675 3_1:-9826859 None:intergenic
GAAGGACAAAGTAAACTTTG+AGG 0.573836 3_1:+9826198 Msa0348030:CDS
GGAGGAATGCCGCTTTCCAA+AGG 0.574058 3_1:-9826397 None:intergenic
CTCCACCATGGTGGCCAACA+GGG 0.575877 3_1:+9826416 Msa0348030:CDS
TTCCCTGTTGGCCACCATGG+TGG 0.581079 3_1:-9826418 None:intergenic
TTCAAGTTGATCAACCATGT+TGG 0.584606 3_1:-9826797 None:intergenic
CGACAGGTACATGTTAAAGA+TGG 0.589384 3_1:+9826081 Msa0348030:intron
TGTATCAGGTAGGTCATACA+AGG 0.591621 3_1:-9826314 None:intergenic
CATTACCAAGACAATCATCA+TGG 0.594402 3_1:+9826862 Msa0348030:CDS
AGAAAGGCTCACCAAATAGG+AGG 0.595686 3_1:+9826934 Msa0348030:CDS
AGAAAACATCCTTTGGAAAG+CGG 0.596755 3_1:+9826388 Msa0348030:CDS
TCCAGGAGTTGGAATCATGT+TGG 0.604429 3_1:-9827562 None:intergenic
ACAATTGAAAAGAAAGACAA+TGG 0.608503 3_1:+9824980 Msa0348030:CDS
AGGGAACGAATCATGGTGGA+AGG 0.608968 3_1:+9826435 Msa0348030:CDS
AGAATTTCTATGATCAACAA+GGG 0.625036 3_1:+9827616 Msa0348030:CDS
AATCTTGTTATTGAGAGTAG+GGG 0.639924 3_1:+9826901 Msa0348030:CDS
ACAATGGCTCGTGCACAAGA+GGG 0.658700 3_1:+9824996 Msa0348030:CDS
AGCTCAAAGAATCAACAACA+TGG 0.679952 3_1:+9826997 Msa0348030:CDS
GCGGCATTCCTCCACCATGG+TGG 0.682914 3_1:+9826407 Msa0348030:CDS
AACCTAATATTGTATCAGGT+AGG 0.686602 3_1:-9826324 None:intergenic
GAATTTCTATGATCAACAAG+GGG 0.696998 3_1:+9827617 Msa0348030:CDS
GGAAACAGATGATTATGATG+TGG 0.698666 3_1:+9826717 Msa0348030:CDS
TATGGAGTAGTACCTGACAA+TGG 0.702873 3_1:+9826133 Msa0348030:CDS
AAAGCGGCATTCCTCCACCA+TGG 0.708484 3_1:+9826404 Msa0348030:CDS
TACCAAGACAATCATCATGG+AGG 0.740488 3_1:+9826865 Msa0348030:CDS
GGCTCTTCGGAAAATCTACG+CGG 0.742332 3_1:+9826169 Msa0348030:CDS
CAACAGGGAACGAATCATGG+TGG 0.754137 3_1:+9826431 Msa0348030:CDS
TGAGGGTTCTGATACCAACA+TGG 0.812240 3_1:+9826783 Msa0348030:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTATATATTAAATTTAA+AGG - chr3_1:9825559-9825578 None:intergenic 0.0%
!!! TCAATAGAAATAAAAATTTT+GGG - chr3_1:9825192-9825211 None:intergenic 10.0%
!!! TTCAATAGAAATAAAAATTT+TGG - chr3_1:9825193-9825212 None:intergenic 10.0%
!! TAACTAAAAATATATTATTG+GGG - chr3_1:9825244-9825263 None:intergenic 10.0%
!! GTAACTAAAAATATATTATT+GGG - chr3_1:9825245-9825264 None:intergenic 10.0%
!! AAAAATTAGCATAAATTAAA+AGG + chr3_1:9825285-9825304 Msa0348030:intron 10.0%
!! TTTACTTCATATTAAAATTA+TGG - chr3_1:9825928-9825947 None:intergenic 10.0%
!!! CAATAGAAATAAAAATTTTG+GGG - chr3_1:9825191-9825210 None:intergenic 15.0%
!! GGTAACTAAAAATATATTAT+TGG - chr3_1:9825246-9825265 None:intergenic 15.0%
!!! CCAAATACAAATATTTTTTT+AGG - chr3_1:9825606-9825625 None:intergenic 15.0%
!! CCTAAAAAAATATTTGTATT+TGG + chr3_1:9825603-9825622 Msa0348030:intron 15.0%
!!! TTACTTAAAAACTAGTTTTT+AGG + chr3_1:9827221-9827240 Msa0348030:intron 15.0%
!!! TACTTAAAAACTAGTTTTTA+GGG + chr3_1:9827222-9827241 Msa0348030:intron 15.0%
!!! CCTGAAAATATTCTTTAAAA+CGG - chr3_1:9825131-9825150 None:intergenic 20.0%
!!! CGTTTTAAAGAATATTTTCA+GGG + chr3_1:9825129-9825148 Msa0348030:intron 20.0%
!!! GTTTTAAAGAATATTTTCAG+GGG + chr3_1:9825130-9825149 Msa0348030:intron 20.0%
!!! TTTGCAAATAAATTTGTTTG+AGG - chr3_1:9825267-9825286 None:intergenic 20.0%
!! ATGTTTAATCTCTTAAAACT+AGG - chr3_1:9825530-9825549 None:intergenic 20.0%
!!! TATTTTAATTCTATCTCTTG+AGG + chr3_1:9825662-9825681 Msa0348030:intron 20.0%
!!! ATATTTTAGTTAGTGAACTA+GGG + chr3_1:9825749-9825768 Msa0348030:intron 20.0%
!! AAGTCAAAGTTATCAAAATT+GGG - chr3_1:9826015-9826034 None:intergenic 20.0%
!! TATTAGTTATGAAGAAAGAT+TGG + chr3_1:9826657-9826676 Msa0348030:CDS 20.0%
! AACAATGCAAAACAAGATAA+CGG + chr3_1:9824822-9824841 Msa0348030:CDS 25.0%
! AAACATAGATATGACTATTG+AGG + chr3_1:9824869-9824888 Msa0348030:CDS 25.0%
! ACAATTGAAAAGAAAGACAA+TGG + chr3_1:9824980-9824999 Msa0348030:CDS 25.0%
!!! CCGTTTTAAAGAATATTTTC+AGG + chr3_1:9825128-9825147 Msa0348030:intron 25.0%
!!! TTTATTTCTATTGAACCACT+AGG + chr3_1:9825198-9825217 Msa0348030:intron 25.0%
! AACCACTAGGTATATTAATA+TGG + chr3_1:9825211-9825230 Msa0348030:intron 25.0%
! TCAATAGTCAAACATAATAG+AGG + chr3_1:9825407-9825426 Msa0348030:intron 25.0%
!! AGCTAGAGAAATTTTTACAA+GGG - chr3_1:9825631-9825650 None:intergenic 25.0%
!! AAGCTAGAGAAATTTTTACA+AGG - chr3_1:9825632-9825651 None:intergenic 25.0%
!!! GGATCATTTTCAAGTTTAAT+TGG + chr3_1:9825716-9825735 Msa0348030:intron 25.0%
!!! CATATTTTAGTTAGTGAACT+AGG + chr3_1:9825748-9825767 Msa0348030:intron 25.0%
! CAAGTCAAAGTTATCAAAAT+TGG - chr3_1:9826016-9826035 None:intergenic 25.0%
!! AAAGTAAACTTTGAGGAAAA+CGG + chr3_1:9826205-9826224 Msa0348030:CDS 25.0%
! TTCACATTGTTCTTCATATT+TGG - chr3_1:9826245-9826264 None:intergenic 25.0%
! ATTGAACCTAATATTGTATC+AGG - chr3_1:9826331-9826350 None:intergenic 25.0%
!!! AAATCATGTGGTTTTTTGTA+TGG - chr3_1:9826493-9826512 None:intergenic 25.0%
! GCAAAATAAATTCAATGCTA+AGG + chr3_1:9826612-9826631 Msa0348030:CDS 25.0%
! AGAATTTCTATGATCAACAA+GGG + chr3_1:9827616-9827635 Msa0348030:CDS 25.0%
AACGGAAAAGAAAAAGAAGA+AGG + chr3_1:9824840-9824859 Msa0348030:CDS 30.0%
AAGTGACAATAAAACATCAC+TGG + chr3_1:9824956-9824975 Msa0348030:CDS 30.0%
AGCCATATTAATATACCTAG+TGG - chr3_1:9825216-9825235 None:intergenic 30.0%
! GCTAGAGAAATTTTTACAAG+GGG - chr3_1:9825630-9825649 None:intergenic 30.0%
! TAAAAATTTCTCTAGCTTCG+CGG + chr3_1:9825634-9825653 Msa0348030:intron 30.0%
TTAAACTTGAAAATGATCCC+TGG - chr3_1:9825715-9825734 None:intergenic 30.0%
AACCTAATATTGTATCAGGT+AGG - chr3_1:9826327-9826346 None:intergenic 30.0%
! TTCCAAAGGATGTTTTCTTT+GGG - chr3_1:9826386-9826405 None:intergenic 30.0%
! TTTCCAAAGGATGTTTTCTT+TGG - chr3_1:9826387-9826406 None:intergenic 30.0%
AAAACCACATGATTTGAAGA+AGG + chr3_1:9826498-9826517 Msa0348030:CDS 30.0%
TTGGAAAATTTGTGTGTTGA+CGG + chr3_1:9826522-9826541 Msa0348030:CDS 30.0%
ACAAACTCATAACTACTTTC+AGG - chr3_1:9826697-9826716 None:intergenic 30.0%
TGAAAGTAGTTATGAGTTTG+TGG + chr3_1:9826696-9826715 Msa0348030:CDS 30.0%
!! ACAATCTTGTTATTGAGAGT+AGG + chr3_1:9826899-9826918 Msa0348030:CDS 30.0%
!! CAATCTTGTTATTGAGAGTA+GGG + chr3_1:9826900-9826919 Msa0348030:CDS 30.0%
!! AATCTTGTTATTGAGAGTAG+GGG + chr3_1:9826901-9826920 Msa0348030:CDS 30.0%
ATGCATGACAAATGTAGATA+GGG - chr3_1:9827181-9827200 None:intergenic 30.0%
! TTTTTCCTATGTAAGTAGCT+CGG - chr3_1:9827302-9827321 None:intergenic 30.0%
! AAGGAGAAGATGTTTCTATT+TGG + chr3_1:9827430-9827449 Msa0348030:intron 30.0%
!! AGATTTGAGGCTGAAAATTT+TGG - chr3_1:9827509-9827528 None:intergenic 30.0%
ATCATTGAAGTGTAGATTTG+AGG - chr3_1:9827522-9827541 None:intergenic 30.0%
CAGAATTTCTATGATCAACA+AGG + chr3_1:9827615-9827634 Msa0348030:CDS 30.0%
GAATTTCTATGATCAACAAG+GGG + chr3_1:9827617-9827636 Msa0348030:CDS 30.0%
! TTCATCTTTAGATGAGTTGA+TGG + chr3_1:9827731-9827750 Msa0348030:CDS 30.0%
!! ACTTATTGATTCTCCTAATG+TGG + chr3_1:9827788-9827807 Msa0348030:CDS 30.0%
CACAAAGTTATGATTTCTCT+TGG + chr3_1:9827814-9827833 Msa0348030:CDS 30.0%
GGAAAAGAAAAAGAAGAAGG+TGG + chr3_1:9824843-9824862 Msa0348030:CDS 35.0%
AGGAACTAGAGACAAAGATA+TGG + chr3_1:9824889-9824908 Msa0348030:CDS 35.0%
AAAATCGCAAGTTAAAGTCC+AGG + chr3_1:9825694-9825713 Msa0348030:intron 35.0%
AAATCGCAAGTTAAAGTCCA+GGG + chr3_1:9825695-9825714 Msa0348030:intron 35.0%
GAAGGACAAAGTAAACTTTG+AGG + chr3_1:9826198-9826217 Msa0348030:CDS 35.0%
TGTGAAGTTTCAACAGTGAA+TGG + chr3_1:9826259-9826278 Msa0348030:CDS 35.0%
GACCTACCTGATACAATATT+AGG + chr3_1:9826322-9826341 Msa0348030:CDS 35.0%
! TCCAAAGGATGTTTTCTTTG+GGG - chr3_1:9826385-9826404 None:intergenic 35.0%
! AGAAAACATCCTTTGGAAAG+CGG + chr3_1:9826388-9826407 Msa0348030:CDS 35.0%
GAAGGAAATGGGATTTAGTA+AGG + chr3_1:9826453-9826472 Msa0348030:CDS 35.0%
!!! TCATGTGGTTTTTTGTATGG+TGG - chr3_1:9826490-9826509 None:intergenic 35.0%
CAAACCTTCTTCAAATCATG+TGG - chr3_1:9826505-9826524 None:intergenic 35.0%
CACATGATTTGAAGAAGGTT+TGG + chr3_1:9826503-9826522 Msa0348030:CDS 35.0%
! CTCCTAATTTTCAGAAGATC+AGG + chr3_1:9826563-9826582 Msa0348030:CDS 35.0%
! CTCCTGATCTTCTGAAAATT+AGG - chr3_1:9826568-9826587 None:intergenic 35.0%
!!! GAATTTATTTTGCAAGCTCC+TGG - chr3_1:9826605-9826624 None:intergenic 35.0%
GGAAACAGATGATTATGATG+TGG + chr3_1:9826717-9826736 Msa0348030:CDS 35.0%
ACCTTTATCATCATGTGATG+AGG + chr3_1:9826765-9826784 Msa0348030:CDS 35.0%
CCTTTATCATCATGTGATGA+GGG + chr3_1:9826766-9826785 Msa0348030:CDS 35.0%
!! TTCAAGTTGATCAACCATGT+TGG - chr3_1:9826800-9826819 None:intergenic 35.0%
!! TGATGATTGTCTTGGTAATG+TGG - chr3_1:9826862-9826881 None:intergenic 35.0%
CATTACCAAGACAATCATCA+TGG + chr3_1:9826862-9826881 Msa0348030:CDS 35.0%
CAACAACAACCAATTCCAAA+TGG + chr3_1:9826963-9826982 Msa0348030:CDS 35.0%
AACAACCAATTCCAAATGGT+TGG + chr3_1:9826967-9826986 Msa0348030:CDS 35.0%
AGCTCAAAGAATCAACAACA+TGG + chr3_1:9826997-9827016 Msa0348030:CDS 35.0%
AATCAGATCACTATTCATGC+AGG + chr3_1:9827066-9827085 Msa0348030:intron 35.0%
ACTATTCATGCAGGTAATCA+TGG + chr3_1:9827075-9827094 Msa0348030:intron 35.0%
GATGCATGACAAATGTAGAT+AGG - chr3_1:9827182-9827201 None:intergenic 35.0%
! TCTTGATTTGATTACCATGC+AGG + chr3_1:9827393-9827412 Msa0348030:intron 35.0%
!! AAATTTTGGTAATCACCAGC+AGG - chr3_1:9827495-9827514 None:intergenic 35.0%
!! TTTTGGTTAAATCCAGGAGT+TGG - chr3_1:9827576-9827595 None:intergenic 35.0%
!!! TGCATGTTTTGGTTAAATCC+AGG - chr3_1:9827582-9827601 None:intergenic 35.0%
TAGAAATTCTGCTCCATTTG+AGG - chr3_1:9827606-9827625 None:intergenic 35.0%
ACATTAACGACTTCAGCATT+AGG - chr3_1:9827672-9827691 None:intergenic 35.0%
! GTTGAGACATTTTCATGCAT+TGG - chr3_1:9827696-9827715 None:intergenic 35.0%
CTCATCTAAAGATGAATCGA+AGG - chr3_1:9827727-9827746 None:intergenic 35.0%
TAACTTTGTGTTGCCACATT+AGG - chr3_1:9827804-9827823 None:intergenic 35.0%
TTCTCTTGGCCAAACAATAT+TGG + chr3_1:9827828-9827847 Msa0348030:CDS 35.0%
TCTCTTGGCCAAACAATATT+GGG + chr3_1:9827829-9827848 Msa0348030:CDS 35.0%
! ATGCATCCCAACGTTGATAA+CGG + chr3_1:9824792-9824811 Msa0348030:CDS 40.0%
!! GCGAATCCTTTTGATAGCAT+GGG + chr3_1:9825152-9825171 Msa0348030:intron 40.0%
!! CGAATCCTTTTGATAGCATG+GGG + chr3_1:9825153-9825172 Msa0348030:intron 40.0%
CGACAGGTACATGTTAAAGA+TGG + chr3_1:9826081-9826100 Msa0348030:intron 40.0%
CAGGTACATGTTAAAGATGG+TGG + chr3_1:9826084-9826103 Msa0348030:intron 40.0%
! GTGGAATTTTGTGATGTTCG+TGG + chr3_1:9826103-9826122 Msa0348030:CDS 40.0%
TATGGAGTAGTACCTGACAA+TGG + chr3_1:9826133-9826152 Msa0348030:CDS 40.0%
!! ATCATTGGTTTGCCATTGTC+AGG - chr3_1:9826148-9826167 None:intergenic 40.0%
GACAATGGCAAACCAATGAT+TGG + chr3_1:9826148-9826167 Msa0348030:CDS 40.0%
! CAAACCAATGATTGGCTCTT+CGG + chr3_1:9826156-9826175 Msa0348030:CDS 40.0%
! TTTTCCGAAGAGCCAATCAT+TGG - chr3_1:9826163-9826182 None:intergenic 40.0%
! TGTTGCTTGATGAGCAAGTA+AGG + chr3_1:9826284-9826303 Msa0348030:CDS 40.0%
TGTATCAGGTAGGTCATACA+AGG - chr3_1:9826317-9826336 None:intergenic 40.0%
!! AAAGGATGTTTTCTTTGGGG+TGG - chr3_1:9826382-9826401 None:intergenic 40.0%
ACCCCAAAGAAAACATCCTT+TGG + chr3_1:9826381-9826400 Msa0348030:CDS 40.0%
! GAATCATGGTGGAAGGAAAT+GGG + chr3_1:9826442-9826461 Msa0348030:CDS 40.0%
!!! GTGGTTTTTTGTATGGTGGT+GGG - chr3_1:9826486-9826505 None:intergenic 40.0%
!!! TGTGGTTTTTTGTATGGTGG+TGG - chr3_1:9826487-9826506 None:intergenic 40.0%
!! GGAGTATTGTTAGACATTCC+AGG + chr3_1:9826584-9826603 Msa0348030:CDS 40.0%
ATGCTAAGGACACAGCTATT+TGG + chr3_1:9826626-9826645 Msa0348030:CDS 40.0%
CCCTCATCACATGATGATAA+AGG - chr3_1:9826769-9826788 None:intergenic 40.0%
TACCAAGACAATCATCATGG+AGG + chr3_1:9826865-9826884 Msa0348030:CDS 40.0%
AACCTCCATGATGATTGTCT+TGG - chr3_1:9826870-9826889 None:intergenic 40.0%
!! TAGGGGTGCTAATAAGAGAA+AGG + chr3_1:9826918-9826937 Msa0348030:CDS 40.0%
AAGAGAAAGGCTCACCAAAT+AGG + chr3_1:9826931-9826950 Msa0348030:CDS 40.0%
! CAACTCCAACCATTTGGAAT+TGG - chr3_1:9826975-9826994 None:intergenic 40.0%
CAATTCCAAATGGTTGGAGT+TGG + chr3_1:9826973-9826992 Msa0348030:CDS 40.0%
! GTGATCTGATTTGCAACAGA+AGG - chr3_1:9827057-9827076 None:intergenic 40.0%
ATGAATCATGTTGTGCAAGC+TGG + chr3_1:9827453-9827472 Msa0348030:intron 40.0%
!! GTTGGAATCATGTTGGTAAC+TGG - chr3_1:9827558-9827577 None:intergenic 40.0%
!! GAAGCACCGTTATCAACGTT+GGG - chr3_1:9824801-9824820 None:intergenic 45.0%
!! TGAAGCACCGTTATCAACGT+TGG - chr3_1:9824802-9824821 None:intergenic 45.0%
! GTGCACAAGAGGGCATATTA+AGG + chr3_1:9825006-9825025 Msa0348030:CDS 45.0%
!! GGCGAATCCTTTTGATAGCA+TGG + chr3_1:9825151-9825170 Msa0348030:intron 45.0%
TGCTACCCCATGCTATCAAA+AGG - chr3_1:9825161-9825180 None:intergenic 45.0%
! TTGATAGCATGGGGTAGCAT+TGG + chr3_1:9825162-9825181 Msa0348030:intron 45.0%
GTGAACTAGGGTAAACTTGC+AGG + chr3_1:9825761-9825780 Msa0348030:intron 45.0%
TCATATTTGGCGATTGCAGC+TGG - chr3_1:9826232-9826251 None:intergenic 45.0%
GGTAGGTCATACAAGGAGTA+AGG - chr3_1:9826310-9826329 None:intergenic 45.0%
! CGAATCATGGTGGAAGGAAA+TGG + chr3_1:9826441-9826460 Msa0348030:CDS 45.0%
! TGAGGGTTCTGATACCAACA+TGG + chr3_1:9826783-9826802 Msa0348030:CDS 45.0%
AGAAAGGCTCACCAAATAGG+AGG + chr3_1:9826934-9826953 Msa0348030:CDS 45.0%
! TGTTGCTGCTTCCTCCTATT+TGG - chr3_1:9826948-9826967 None:intergenic 45.0%
AGCTTCCAACTCCAACCATT+TGG - chr3_1:9826981-9827000 None:intergenic 45.0%
ACCAACCGAGCTACTTACAT+AGG + chr3_1:9827294-9827313 Msa0348030:intron 45.0%
TCCTATGTAAGTAGCTCGGT+TGG - chr3_1:9827298-9827317 None:intergenic 45.0%
TACCATGCAGGTAATGACTC+AGG + chr3_1:9827405-9827424 Msa0348030:intron 45.0%
AACATGATTCCTACTCCTGC+TGG + chr3_1:9827477-9827496 Msa0348030:CDS 45.0%
ACCAACATGATTCCAACTCC+TGG + chr3_1:9827561-9827580 Msa0348030:CDS 45.0%
TCCAGGAGTTGGAATCATGT+TGG - chr3_1:9827565-9827584 None:intergenic 45.0%
!! CCATTTGAGGCTGCATGTTT+TGG - chr3_1:9827593-9827612 None:intergenic 45.0%
CCAAAACATGCAGCCTCAAA+TGG + chr3_1:9827590-9827609 Msa0348030:CDS 45.0%
ACAATGGCTCGTGCACAAGA+GGG + chr3_1:9824996-9825015 Msa0348030:CDS 50.0%
ACACTACGTGTCAGCTCAAC+GGG - chr3_1:9826061-9826080 None:intergenic 50.0%
GACACTACGTGTCAGCTCAA+CGG - chr3_1:9826062-9826081 None:intergenic 50.0%
GATGTTCGTGGCTTCGTCTA+TGG + chr3_1:9826115-9826134 Msa0348030:CDS 50.0%
GGCTCTTCGGAAAATCTACG+CGG + chr3_1:9826169-9826188 Msa0348030:CDS 50.0%
CTTCGGAAAATCTACGCGGT+TGG + chr3_1:9826173-9826192 Msa0348030:CDS 50.0%
!! AAATCTACGCGGTTGGTGGA+AGG + chr3_1:9826180-9826199 Msa0348030:CDS 50.0%
CACCATGATTCGTTCCCTGT+TGG - chr3_1:9826433-9826452 None:intergenic 50.0%
CAACAGGGAACGAATCATGG+TGG + chr3_1:9826431-9826450 Msa0348030:CDS 50.0%
! AGGGAACGAATCATGGTGGA+AGG + chr3_1:9826435-9826454 Msa0348030:CDS 50.0%
GCTTGCTCATGTTGGTCAAG+AGG - chr3_1:9827027-9827046 None:intergenic 50.0%
! GTACTGCAGCTTGCTCATGT+TGG - chr3_1:9827035-9827054 None:intergenic 50.0%
CTCCTGAGTCATTACCTGCA+TGG - chr3_1:9827410-9827429 None:intergenic 50.0%
GCAGGTAATGACTCAGGAGA+AGG + chr3_1:9827411-9827430 Msa0348030:intron 50.0%
TGGTAATCACCAGCAGGAGT+AGG - chr3_1:9827489-9827508 None:intergenic 50.0%
GACAATGGCTCGTGCACAAG+AGG + chr3_1:9824995-9825014 Msa0348030:CDS 55.0%
CACTACGTGTCAGCTCAACG+GGG - chr3_1:9826060-9826079 None:intergenic 55.0%
!! AGCTGACACGTAGTGTCGAC+AGG + chr3_1:9826065-9826084 Msa0348030:intron 55.0%
CGGAAAATCTACGCGGTTGG+TGG + chr3_1:9826176-9826195 Msa0348030:CDS 55.0%
!! GGAGGAATGCCGCTTTCCAA+AGG - chr3_1:9826400-9826419 None:intergenic 55.0%
AAAGCGGCATTCCTCCACCA+TGG + chr3_1:9826404-9826423 Msa0348030:CDS 55.0%
GGCCAACAGGGAACGAATCA+TGG + chr3_1:9826428-9826447 Msa0348030:CDS 55.0%
CTCCACCATGGTGGCCAACA+GGG + chr3_1:9826416-9826435 Msa0348030:CDS 60.0%
TTCCCTGTTGGCCACCATGG+TGG - chr3_1:9826421-9826440 None:intergenic 60.0%
TCGTTCCCTGTTGGCCACCA+TGG - chr3_1:9826424-9826443 None:intergenic 60.0%
GCGGCATTCCTCCACCATGG+TGG + chr3_1:9826407-9826426 Msa0348030:CDS 65.0%
CCTGTTGGCCACCATGGTGG+AGG - chr3_1:9826418-9826437 None:intergenic 65.0%
CCTCCACCATGGTGGCCAAC+AGG + chr3_1:9826415-9826434 Msa0348030:CDS 65.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 9824792 9827857 9824792 ID=Msa0348030;Name=Msa0348030
chr3_1 mRNA 9824792 9827857 9824792 ID=Msa0348030-mRNA-1;Parent=Msa0348030;Name=Msa0348030-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0|0|0|1|1|1|3|0|517
chr3_1 exon 9824792 9825027 9824792 ID=Msa0348030-mRNA-1:exon:2764;Parent=Msa0348030-mRNA-1
chr3_1 exon 9826087 9827018 9826087 ID=Msa0348030-mRNA-1:exon:2765;Parent=Msa0348030-mRNA-1
chr3_1 exon 9827472 9827857 9827472 ID=Msa0348030-mRNA-1:exon:2766;Parent=Msa0348030-mRNA-1
chr3_1 CDS 9824792 9825027 9824792 ID=Msa0348030-mRNA-1:cds;Parent=Msa0348030-mRNA-1
chr3_1 CDS 9826087 9827018 9826087 ID=Msa0348030-mRNA-1:cds;Parent=Msa0348030-mRNA-1
chr3_1 CDS 9827472 9827857 9827472 ID=Msa0348030-mRNA-1:cds;Parent=Msa0348030-mRNA-1
Gene Sequence

>Msa0348030

ATGCATCCCAACGTTGATAACGGTGCTTCAAACAATGCAAAACAAGATAACGGAAAAGAAAAAGAAGAAGGTGGTGAAAACATAGATATGACTATTGAGGAACTAGAGACAAAGATATGGAAAGATAAAATGCTCTTAAAGAAGATGAAGTTAAAAAGAATTCAAAGTGACAATAAAACATCACTGGAACAATTGAAAAGAAAGACAATGGCTCGTGCACAAGAGGGCATATTAAGGTACATGTTAAAGATGGTGGAATTTTGTGATGTTCGTGGCTTCGTCTATGGAGTAGTACCTGACAATGGCAAACCAATGATTGGCTCTTCGGAAAATCTACGCGGTTGGTGGAAGGACAAAGTAAACTTTGAGGAAAACGGTCCAGCTGCAATCGCCAAATATGAAGAACAATGTGAAGTTTCAACAGTGAATGGCATGTTGCTTGATGAGCAAGTAAGGCCTTACTCCTTGTATGACCTACCTGATACAATATTAGGTTCAATTGTATCATCATTAATGCAACACTGCGATCCACCCCAAAGAAAACATCCTTTGGAAAGCGGCATTCCTCCACCATGGTGGCCAACAGGGAACGAATCATGGTGGAAGGAAATGGGATTTAGTAAGGATCTTGTCCCACCACCATACAAAAAACCACATGATTTGAAGAAGGTTTGGAAAATTTGTGTGTTGACGGCTATTATCAAACACATATCTCCTAATTTTCAGAAGATCAGGAGTATTGTTAGACATTCCAGGAGCTTGCAAAATAAATTCAATGCTAAGGACACAGCTATTTGGTTAGCTGTTATTAGTTATGAAGAAAGATTGGCAAGAGAAATGTATCCTGAAAGTAGTTATGAGTTTGTGGAAACAGATGATTATGATGTGGAATGTGATGAGCACAATCTAGCAAAACCTTTATCATCATGTGATGAGGGTTCTGATACCAACATGGTTGATCAACTTGAAGAAACTTCTTCTCATCTCGAGACTTCGATATGCATTGATCCACATTACCAAGACAATCATCATGGAGGTTCTTCAATGAACAATCTTGTTATTGAGAGTAGGGGTGCTAATAAGAGAAAGGCTCACCAAATAGGAGGAAGCAGCAACAACAACCAATTCCAAATGGTTGGAGTTGGAAGCTCAAAGAATCAACAACATGCTGGCAACATGATTCCTACTCCTGCTGGTGATTACCAAAATTTTCAGCCTCAAATCTACACTTCAATGATGAATACTTCTTTGCCAGTTACCAACATGATTCCAACTCCTGGATTTAACCAAAACATGCAGCCTCAAATGGAGCAGAATTTCTATGATCAACAAGGGGATGCAAATAGCAATTACAACTACAAAGTGCCTAATGCTGAAGTCGTTAATGTTCCAATGCATGAAAATGTCTCAACAATGACAACCTTCGATTCATCTTTAGATGAGTTGATGGCATTCAATTCTCAATCTGATGTTGATGCTTATGCACTTATTGATTCTCCTAATGTGGCAACACAAAGTTATGATTTCTCTTGGCCAAACAATATTGGGAATTAA
Protein sequence

>Msa0348030

MHPNVDNGASNNAKQDNGKEKEEGGENIDMTIEELETKIWKDKMLLKKMKLKRIQSDNKTSLEQLKRKTMARAQEGILRYMLKMVEFCDVRGFVYGVVPDNGKPMIGSSENLRGWWKDKVNFEENGPAAIAKYEEQCEVSTVNGMLLDEQVRPYSLYDLPDTILGSIVSSLMQHCDPPQRKHPLESGIPPPWWPTGNESWWKEMGFSKDLVPPPYKKPHDLKKVWKICVLTAIIKHISPNFQKIRSIVRHSRSLQNKFNAKDTAIWLAVISYEERLAREMYPESSYEFVETDDYDVECDEHNLAKPLSSCDEGSDTNMVDQLEETSSHLETSICIDPHYQDNHHGGSSMNNLVIESRGANKRKAHQIGGSSNNNQFQMVGVGSSKNQQHAGNMIPTPAGDYQNFQPQIYTSMMNTSLPVTNMIPTPGFNQNMQPQMEQNFYDQQGDANSNYNYKVPNAEVVNVPMHENVSTMTTFDSSLDELMAFNSQSDVDAYALIDSPNVATQSYDFSWPNNIGN*