AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0348740

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0348740 MtrunA17_Chr3g0081631 96.703 91 3 0 1 91 1 91 9.70e-63 184
Msa0348740 MtrunA17_Chr7g0255841 67.500 80 25 1 12 91 14 92 1.20e-34 114
Msa0348740 MtrunA17_Chr3g0141601 58.242 91 33 2 4 90 1 90 1.55e-31 105
Msa0348740 MtrunA17_Chr1g0155181 58.621 87 29 3 4 84 1 86 6.07e-30 101
Msa0348740 MtrunA17_Chr6g0449111 61.333 75 28 1 1 75 1 74 1.04e-29 100
Msa0348740 MtrunA17_Chr3g0144631 61.728 81 24 2 1 75 1 80 2.42e-28 97.8
Msa0348740 MtrunA17_Chr5g0404301 59.375 64 24 1 8 71 2 63 6.38e-23 83.6
Msa0348740 MtrunA17_Chr8g0373111 56.716 67 27 1 7 73 2 66 9.57e-23 83.2
Msa0348740 MtrunA17_Chr8g0373101 59.375 64 24 1 8 71 3 64 9.57e-23 83.2
Msa0348740 MtrunA17_Chr8g0373121 56.716 67 27 1 7 73 2 66 9.57e-23 83.2
Msa0348740 MtrunA17_Chr8g0373131 56.716 67 27 1 7 73 2 66 1.00e-22 83.2
Msa0348740 MtrunA17_Chr5g0404101 47.945 73 37 1 1 73 10 81 3.28e-20 77.4
Msa0348740 MtrunA17_Chr5g0436501 49.315 73 36 1 1 73 18 89 3.79e-17 73.6
Msa0348740 MtrunA17_Chr5g0429521 47.619 84 32 2 9 81 28 110 2.47e-16 71.6
Msa0348740 MtrunA17_Chr3g0141641 38.462 91 46 2 9 90 2 91 1.33e-15 65.9
Msa0348740 MtrunA17_Chr8g0364741 43.421 76 42 1 1 76 1 75 1.73e-15 66.2
Msa0348740 MtrunA17_Chr3g0104071 44.944 89 37 2 3 80 36 123 6.76e-15 67.8
Msa0348740 MtrunA17_Chr7g0241761 44.706 85 37 2 10 85 35 118 3.79e-14 65.9
Msa0348740 MtrunA17_Chr1g0162081 44.000 75 41 1 1 75 3 76 4.18e-14 65.5
Msa0348740 MtrunA17_Chr8g0365011 46.377 69 36 1 7 75 5 72 8.57e-14 64.7
Msa0348740 MtrunA17_Chr7g0270711 52.542 59 28 0 10 68 11 69 9.23e-14 60.8
Msa0348740 MtrunA17_Chr1g0194071 50.877 57 28 0 10 66 11 67 9.71e-14 60.8
Msa0348740 MtrunA17_Chr4g0062741 44.737 76 37 3 4 75 1 75 1.99e-12 60.8
Msa0348740 MtrunA17_Chr1g0210471 39.394 66 39 1 10 75 24 88 1.56e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0348740 AT4G39250.1 60.227 88 28 2 10 91 14 100 2.84e-33 110
Msa0348740 AT2G21650.1 60.674 89 27 2 10 91 14 101 4.58e-33 110
Msa0348740 AT1G75250.2 52.632 95 36 3 3 90 5 97 1.93e-26 93.6
Msa0348740 AT1G75250.1 61.972 71 26 1 3 73 5 74 3.62e-25 91.3
Msa0348740 AT1G19510.1 59.375 64 25 1 13 76 15 77 3.69e-21 80.5
Msa0348740 AT2G18328.1 60.938 64 24 1 13 76 14 76 9.34e-21 78.6
Msa0348740 AT4G36570.1 68.889 45 13 1 10 54 12 55 9.02e-17 68.2
Msa0348740 AT5G58900.1 50.685 73 35 1 1 73 25 96 2.86e-16 71.6
Msa0348740 AT2G38090.1 50.649 77 31 2 10 80 29 104 4.78e-16 71.2
Msa0348740 AT5G05790.2 49.351 77 36 2 7 81 28 103 5.74e-16 70.9
Msa0348740 AT5G05790.1 49.351 77 36 2 7 81 28 103 5.74e-16 70.9
Msa0348740 AT3G11280.2 48.438 64 32 1 10 73 33 95 1.77e-13 63.9
Msa0348740 AT3G11280.1 48.438 64 32 1 10 73 33 95 1.77e-13 63.9
Msa0348740 AT5G23650.1 45.833 72 38 1 4 75 7 77 4.64e-12 60.5
Msa0348740 AT5G08520.1 44.928 69 37 1 10 78 12 79 5.33e-11 57.4

Find 57 sgRNAs with CRISPR-Local

Find 186 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTCCAATGTTGTTGTAATT+TGG 0.068098 3_1:-11563357 None:intergenic
CTCTAATTCTTCTTATAAAA+AGG 0.168485 3_1:+11562929 Msa0348740:five_prime_UTR
CTCCAATGTTGTTGTAATTT+GGG 0.246535 3_1:-11563356 None:intergenic
TGTCCCACTTTCTTGTCATT+TGG 0.262336 3_1:+11564271 Msa0348740:three_prime_UTR
GACACTATGAACACCTTCTA+AGG 0.262540 3_1:+11563298 Msa0348740:CDS
CACATGTGGTAATAATTAAA+TGG 0.300192 3_1:-11564351 None:intergenic
TGTTGACACCATTTGGTTAC+TGG 0.305364 3_1:-11564095 None:intergenic
CAAGTTGGAATGAAAATCTT+TGG 0.319427 3_1:-11562881 None:intergenic
CCACAACCTTCAAAATCTTA+AGG 0.319647 3_1:+11563052 Msa0348740:five_prime_UTR
CATTTGGTTACTGGAGCTTC+AGG 0.328788 3_1:-11564086 None:intergenic
TGAACCTTGGTTTCAAGAAA+TGG 0.338544 3_1:+11563128 Msa0348740:exon
CACAACCTTCAAAATCTTAA+GGG 0.341805 3_1:+11563053 Msa0348740:five_prime_UTR
TATTTGTTGTTGACACCATT+TGG 0.373580 3_1:-11564102 None:intergenic
GATGTTAGGCATATTGAATT+AGG 0.397381 3_1:+11563321 Msa0348740:CDS
TGGAAGTTAACAAAACTTGT+AGG 0.402986 3_1:-11563032 None:intergenic
TATGCCTAACATCCCTTAGA+AGG 0.404703 3_1:-11563311 None:intergenic
CAATGTCAGCATCTGGTTCA+TGG 0.413232 3_1:+11563154 Msa0348740:CDS
AAGAATAAGGCTTAAGGAAG+AGG 0.429086 3_1:-11562981 None:intergenic
TAGGCTATTCAAAATGTTGA+AGG 0.431834 3_1:-11563013 None:intergenic
TGTGTAAAGAATAAGGCTTA+AGG 0.455726 3_1:-11562987 None:intergenic
TAGAGGGCGTGTGGAAGGTG+TGG 0.460077 3_1:-11562911 None:intergenic
GAAAGTGGGACAAGGAATCA+TGG 0.463284 3_1:-11564260 None:intergenic
AGAGGGCGTGTGGAAGGTGT+GGG 0.464590 3_1:-11562910 None:intergenic
AACACCTTCTAAGGGATGTT+AGG 0.467379 3_1:+11563307 Msa0348740:CDS
TAGTGTCTCTTAACATCCTC+AGG 0.475467 3_1:-11563282 None:intergenic
AGAATTAGAGGGCGTGTGGA+AGG 0.485502 3_1:-11562916 None:intergenic
ATGGCATCAATGTCAGCATC+TGG 0.486683 3_1:+11563147 Msa0348740:CDS
CCAAATTACAACAACATTGG+AGG 0.491542 3_1:+11563357 Msa0348740:CDS
ACCAACAGCTTGAGCAACAT+TGG 0.499603 3_1:-11563247 None:intergenic
AGGACAATAAAGCATTTGAA+AGG 0.506069 3_1:+11563184 Msa0348740:CDS
GTTGCTCAAGCTGTTGGTGG+TGG 0.513723 3_1:+11563252 Msa0348740:CDS
TTGAAGAGAGGAAGCAAGGT+TGG 0.517778 3_1:-11562953 None:intergenic
TCCAATGTTGCTCAAGCTGT+TGG 0.520440 3_1:+11563246 Msa0348740:CDS
CTGAAGCTCCAGTAACCAAA+TGG 0.524122 3_1:+11564087 Msa0348740:exon
TTCCCAAATTACAACAACAT+TGG 0.529125 3_1:+11563354 Msa0348740:CDS
GGACAATAAAGCATTTGAAA+GGG 0.535274 3_1:+11563185 Msa0348740:CDS
GCAACATTGGACCAACGGTC+AGG 0.535547 3_1:-11563234 None:intergenic
GATGAAAAGAGATTATAGTA+AGG 0.535884 3_1:-11564376 None:intergenic
AGGTGTGGGGTAGAGCAAGT+TGG 0.539815 3_1:-11562896 None:intergenic
CTTGTCATTTGGAATATCAA+CGG 0.541338 3_1:+11564282 Msa0348740:three_prime_UTR
AATGTTGCTCAAGCTGTTGG+TGG 0.546053 3_1:+11563249 Msa0348740:CDS
TATTCCAAATGACAAGAAAG+TGG 0.550193 3_1:-11564275 None:intergenic
AAAGTGGGACAAGGAATCAT+GGG 0.550936 3_1:-11564259 None:intergenic
TTTCACTAATGCTACATCAC+TGG 0.559072 3_1:-11564212 None:intergenic
AAGAAGAATTAGAGGGCGTG+TGG 0.560374 3_1:-11562920 None:intergenic
GATGCCATTTCTTGAAACCA+AGG 0.568023 3_1:-11563132 None:intergenic
ATCTGGTTCATGGAGTGCTA+AGG 0.583163 3_1:+11563164 Msa0348740:CDS
GAGGGCGTGTGGAAGGTGTG+GGG 0.588905 3_1:-11562909 None:intergenic
ATAAAGACACACCTGACCGT+TGG 0.596639 3_1:+11563223 Msa0348740:CDS
ATTCCAAATGACAAGAAAGT+GGG 0.605269 3_1:-11564274 None:intergenic
ACACTATGAACACCTTCTAA+GGG 0.610400 3_1:+11563299 Msa0348740:CDS
TTGCTCAAGCTGTTGGTGGT+GGG 0.637169 3_1:+11563253 Msa0348740:CDS
TGGTGGTGGGAAGACTCCTG+AGG 0.637206 3_1:+11563266 Msa0348740:CDS
CTTGAGCAACATTGGACCAA+CGG 0.640722 3_1:-11563239 None:intergenic
AATGACAAGAAAGTGGGACA+AGG 0.665746 3_1:-11564268 None:intergenic
CAACATTGGAGGCTATGATG+AGG 0.684494 3_1:+11563368 Msa0348740:CDS
ATTATAGTAAGGTACACATG+TGG 0.707926 3_1:-11564365 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTCTTATAAACATATATT+AGG + chr3_1:11563419-11563438 Msa0348740:intron 10.0%
!!! TATTTATATGTACTAATTTT+TGG + chr3_1:11563815-11563834 Msa0348740:intron 10.0%
!!! ATTTATATGTACTAATTTTT+GGG + chr3_1:11563816-11563835 Msa0348740:intron 10.0%
!! ATGTACTAAAAAAATATATT+AGG + chr3_1:11564458-11564477 Msa0348740:three_prime_UTR 10.0%
!!! TTTTCTTATAAACATATATT+AGG + chr3_1:11563419-11563438 Msa0348740:intron 10.0%
!!! TATTTATATGTACTAATTTT+TGG + chr3_1:11563815-11563834 Msa0348740:intron 10.0%
!!! ATTTATATGTACTAATTTTT+GGG + chr3_1:11563816-11563835 Msa0348740:intron 10.0%
!! ATGTACTAAAAAAATATATT+AGG + chr3_1:11564458-11564477 Msa0348740:three_prime_UTR 10.0%
!!! TTTTTATAAGAAGAATTAGA+GGG - chr3_1:11562930-11562949 None:intergenic 15.0%
!!! TATTGATTGTATATGAATAA+GGG - chr3_1:11563647-11563666 None:intergenic 15.0%
!!! TTATTGATTGTATATGAATA+AGG - chr3_1:11563648-11563667 None:intergenic 15.0%
!!! GTTTTTATTTTTAGAATCAT+TGG + chr3_1:11564155-11564174 Msa0348740:three_prime_UTR 15.0%
!!! ATGAAGCTATAATTTTATTA+AGG - chr3_1:11564326-11564345 None:intergenic 15.0%
!!! TTTTTATAAGAAGAATTAGA+GGG - chr3_1:11562930-11562949 None:intergenic 15.0%
!!! TATTGATTGTATATGAATAA+GGG - chr3_1:11563647-11563666 None:intergenic 15.0%
!!! TTATTGATTGTATATGAATA+AGG - chr3_1:11563648-11563667 None:intergenic 15.0%
!!! GTTTTTATTTTTAGAATCAT+TGG + chr3_1:11564155-11564174 Msa0348740:three_prime_UTR 15.0%
!!! ATGAAGCTATAATTTTATTA+AGG - chr3_1:11564326-11564345 None:intergenic 15.0%
!!! CTTTTTATAAGAAGAATTAG+AGG - chr3_1:11562931-11562950 None:intergenic 20.0%
!! CTCTAATTCTTCTTATAAAA+AGG + chr3_1:11562929-11562948 Msa0348740:five_prime_UTR 20.0%
!!! ACAAAAACAAGAAATTTGTT+TGG + chr3_1:11563096-11563115 Msa0348740:five_prime_UTR 20.0%
!! TCTTATAAACATATATTAGG+AGG + chr3_1:11563422-11563441 Msa0348740:intron 20.0%
!!! AGAATTTGATAGCATAAATT+CGG - chr3_1:11563753-11563772 None:intergenic 20.0%
!! ACACATGTTAAACAAAAAAT+TGG - chr3_1:11563908-11563927 None:intergenic 20.0%
!!! ATTTTTAGAATCATTGGATT+AGG + chr3_1:11564161-11564180 Msa0348740:three_prime_UTR 20.0%
!!! GCTATAATTTTATTAAGGTT+GGG - chr3_1:11564321-11564340 None:intergenic 20.0%
!!! AGCTATAATTTTATTAAGGT+TGG - chr3_1:11564322-11564341 None:intergenic 20.0%
!!! AACACATTATCTTCTTTTTT+TGG - chr3_1:11564438-11564457 None:intergenic 20.0%
!!! CTTTTTATAAGAAGAATTAG+AGG - chr3_1:11562931-11562950 None:intergenic 20.0%
!! CTCTAATTCTTCTTATAAAA+AGG + chr3_1:11562929-11562948 Msa0348740:five_prime_UTR 20.0%
!!! ACAAAAACAAGAAATTTGTT+TGG + chr3_1:11563096-11563115 Msa0348740:five_prime_UTR 20.0%
!! TCTTATAAACATATATTAGG+AGG + chr3_1:11563422-11563441 Msa0348740:intron 20.0%
!!! AGAATTTGATAGCATAAATT+CGG - chr3_1:11563753-11563772 None:intergenic 20.0%
!! ACACATGTTAAACAAAAAAT+TGG - chr3_1:11563908-11563927 None:intergenic 20.0%
!!! ATTTTTAGAATCATTGGATT+AGG + chr3_1:11564161-11564180 Msa0348740:three_prime_UTR 20.0%
!!! GCTATAATTTTATTAAGGTT+GGG - chr3_1:11564321-11564340 None:intergenic 20.0%
!!! AGCTATAATTTTATTAAGGT+TGG - chr3_1:11564322-11564341 None:intergenic 20.0%
!!! AACACATTATCTTCTTTTTT+TGG - chr3_1:11564438-11564457 None:intergenic 20.0%
!!! AAGGTTTTGTGTAAAGAATA+AGG - chr3_1:11562997-11563016 None:intergenic 25.0%
! ACGCTAAATAAGTATTATGA+AGG - chr3_1:11563468-11563487 None:intergenic 25.0%
! ATAGTGAGAATGAAAAATGA+TGG + chr3_1:11563714-11563733 Msa0348740:intron 25.0%
!! GAATTTGATAGCATAAATTC+GGG - chr3_1:11563752-11563771 None:intergenic 25.0%
!! GAAGTAAAAAAGTGTAAGTT+AGG - chr3_1:11563931-11563950 None:intergenic 25.0%
! CACATGTGGTAATAATTAAA+TGG - chr3_1:11564354-11564373 None:intergenic 25.0%
! GATGAAAAGAGATTATAGTA+AGG - chr3_1:11564379-11564398 None:intergenic 25.0%
!!! AAGGTTTTGTGTAAAGAATA+AGG - chr3_1:11562997-11563016 None:intergenic 25.0%
! ACGCTAAATAAGTATTATGA+AGG - chr3_1:11563468-11563487 None:intergenic 25.0%
! ATAGTGAGAATGAAAAATGA+TGG + chr3_1:11563714-11563733 Msa0348740:intron 25.0%
!! GAATTTGATAGCATAAATTC+GGG - chr3_1:11563752-11563771 None:intergenic 25.0%
!! GAAGTAAAAAAGTGTAAGTT+AGG - chr3_1:11563931-11563950 None:intergenic 25.0%
! CACATGTGGTAATAATTAAA+TGG - chr3_1:11564354-11564373 None:intergenic 25.0%
! GATGAAAAGAGATTATAGTA+AGG - chr3_1:11564379-11564398 None:intergenic 25.0%
! CAAGTTGGAATGAAAATCTT+TGG - chr3_1:11562884-11562903 None:intergenic 30.0%
TGTGTAAAGAATAAGGCTTA+AGG - chr3_1:11562990-11563009 None:intergenic 30.0%
TAGGCTATTCAAAATGTTGA+AGG - chr3_1:11563016-11563035 None:intergenic 30.0%
TGGAAGTTAACAAAACTTGT+AGG - chr3_1:11563035-11563054 None:intergenic 30.0%
CACAACCTTCAAAATCTTAA+GGG + chr3_1:11563053-11563072 Msa0348740:five_prime_UTR 30.0%
! AGGACAATAAAGCATTTGAA+AGG + chr3_1:11563184-11563203 Msa0348740:CDS 30.0%
! GGACAATAAAGCATTTGAAA+GGG + chr3_1:11563185-11563204 Msa0348740:CDS 30.0%
GATGTTAGGCATATTGAATT+AGG + chr3_1:11563321-11563340 Msa0348740:CDS 30.0%
TTCCCAAATTACAACAACAT+TGG + chr3_1:11563354-11563373 Msa0348740:CDS 30.0%
CTCCAATGTTGTTGTAATTT+GGG - chr3_1:11563359-11563378 None:intergenic 30.0%
TCAAGCATAATTCATAAGAG+TGG + chr3_1:11563787-11563806 Msa0348740:intron 30.0%
!!! GTACTAATTTTTGGGTTAAG+TGG + chr3_1:11563824-11563843 Msa0348740:intron 30.0%
!! TATTTGTTGTTGACACCATT+TGG - chr3_1:11564105-11564124 None:intergenic 30.0%
ATTCCAAATGACAAGAAAGT+GGG - chr3_1:11564277-11564296 None:intergenic 30.0%
TATTCCAAATGACAAGAAAG+TGG - chr3_1:11564278-11564297 None:intergenic 30.0%
! CTTGTCATTTGGAATATCAA+CGG + chr3_1:11564282-11564301 Msa0348740:three_prime_UTR 30.0%
ATTATAGTAAGGTACACATG+TGG - chr3_1:11564368-11564387 None:intergenic 30.0%
! CAAGTTGGAATGAAAATCTT+TGG - chr3_1:11562884-11562903 None:intergenic 30.0%
TGTGTAAAGAATAAGGCTTA+AGG - chr3_1:11562990-11563009 None:intergenic 30.0%
TAGGCTATTCAAAATGTTGA+AGG - chr3_1:11563016-11563035 None:intergenic 30.0%
TGGAAGTTAACAAAACTTGT+AGG - chr3_1:11563035-11563054 None:intergenic 30.0%
CACAACCTTCAAAATCTTAA+GGG + chr3_1:11563053-11563072 Msa0348740:five_prime_UTR 30.0%
! AGGACAATAAAGCATTTGAA+AGG + chr3_1:11563184-11563203 Msa0348740:CDS 30.0%
! GGACAATAAAGCATTTGAAA+GGG + chr3_1:11563185-11563204 Msa0348740:CDS 30.0%
GATGTTAGGCATATTGAATT+AGG + chr3_1:11563321-11563340 Msa0348740:CDS 30.0%
TTCCCAAATTACAACAACAT+TGG + chr3_1:11563354-11563373 Msa0348740:CDS 30.0%
CTCCAATGTTGTTGTAATTT+GGG - chr3_1:11563359-11563378 None:intergenic 30.0%
TCAAGCATAATTCATAAGAG+TGG + chr3_1:11563787-11563806 Msa0348740:intron 30.0%
!!! GTACTAATTTTTGGGTTAAG+TGG + chr3_1:11563824-11563843 Msa0348740:intron 30.0%
!! TATTTGTTGTTGACACCATT+TGG - chr3_1:11564105-11564124 None:intergenic 30.0%
ATTCCAAATGACAAGAAAGT+GGG - chr3_1:11564277-11564296 None:intergenic 30.0%
TATTCCAAATGACAAGAAAG+TGG - chr3_1:11564278-11564297 None:intergenic 30.0%
! CTTGTCATTTGGAATATCAA+CGG + chr3_1:11564282-11564301 Msa0348740:three_prime_UTR 30.0%
ATTATAGTAAGGTACACATG+TGG - chr3_1:11564368-11564387 None:intergenic 30.0%
AAGAATAAGGCTTAAGGAAG+AGG - chr3_1:11562984-11563003 None:intergenic 35.0%
!! CCTTAAGATTTTGAAGGTTG+TGG - chr3_1:11563055-11563074 None:intergenic 35.0%
CCACAACCTTCAAAATCTTA+AGG + chr3_1:11563052-11563071 Msa0348740:five_prime_UTR 35.0%
! ATGAGCCCTTAAGATTTTGA+AGG - chr3_1:11563061-11563080 None:intergenic 35.0%
!!! TTGGACTTAGTTTTGAACCT+TGG + chr3_1:11563115-11563134 Msa0348740:five_prime_UTR 35.0%
TGAACCTTGGTTTCAAGAAA+TGG + chr3_1:11563128-11563147 Msa0348740:exon 35.0%
ACACTATGAACACCTTCTAA+GGG + chr3_1:11563299-11563318 Msa0348740:CDS 35.0%
CCTCCAATGTTGTTGTAATT+TGG - chr3_1:11563360-11563379 None:intergenic 35.0%
CCAAATTACAACAACATTGG+AGG + chr3_1:11563357-11563376 Msa0348740:CDS 35.0%
!! AACTCATATCGCTGATTTAC+TGG + chr3_1:11563491-11563510 Msa0348740:intron 35.0%
AGCATAAATTCGGGTATGTT+CGG - chr3_1:11563743-11563762 None:intergenic 35.0%
!!! TTTTGGGTTAAGTGGAGAAT+CGG + chr3_1:11563832-11563851 Msa0348740:intron 35.0%
TTTCACTAATGCTACATCAC+TGG - chr3_1:11564215-11564234 None:intergenic 35.0%
AAGAATAAGGCTTAAGGAAG+AGG - chr3_1:11562984-11563003 None:intergenic 35.0%
!! CCTTAAGATTTTGAAGGTTG+TGG - chr3_1:11563055-11563074 None:intergenic 35.0%
CCACAACCTTCAAAATCTTA+AGG + chr3_1:11563052-11563071 Msa0348740:five_prime_UTR 35.0%
! ATGAGCCCTTAAGATTTTGA+AGG - chr3_1:11563061-11563080 None:intergenic 35.0%
!!! TTGGACTTAGTTTTGAACCT+TGG + chr3_1:11563115-11563134 Msa0348740:five_prime_UTR 35.0%
TGAACCTTGGTTTCAAGAAA+TGG + chr3_1:11563128-11563147 Msa0348740:exon 35.0%
ACACTATGAACACCTTCTAA+GGG + chr3_1:11563299-11563318 Msa0348740:CDS 35.0%
CCTCCAATGTTGTTGTAATT+TGG - chr3_1:11563360-11563379 None:intergenic 35.0%
CCAAATTACAACAACATTGG+AGG + chr3_1:11563357-11563376 Msa0348740:CDS 35.0%
!! AACTCATATCGCTGATTTAC+TGG + chr3_1:11563491-11563510 Msa0348740:intron 35.0%
AGCATAAATTCGGGTATGTT+CGG - chr3_1:11563743-11563762 None:intergenic 35.0%
!!! TTTTGGGTTAAGTGGAGAAT+CGG + chr3_1:11563832-11563851 Msa0348740:intron 35.0%
TTTCACTAATGCTACATCAC+TGG - chr3_1:11564215-11564234 None:intergenic 35.0%
!! GATTTTGAAGAGAGGAAGCA+AGG - chr3_1:11562960-11562979 None:intergenic 40.0%
!! GAAGAGGTGATTTTGAAGAG+AGG - chr3_1:11562968-11562987 None:intergenic 40.0%
! GATGCCATTTCTTGAAACCA+AGG - chr3_1:11563135-11563154 None:intergenic 40.0%
TAGTGTCTCTTAACATCCTC+AGG - chr3_1:11563285-11563304 None:intergenic 40.0%
GACACTATGAACACCTTCTA+AGG + chr3_1:11563298-11563317 Msa0348740:CDS 40.0%
! AACACCTTCTAAGGGATGTT+AGG + chr3_1:11563307-11563326 Msa0348740:CDS 40.0%
TATGCCTAACATCCCTTAGA+AGG - chr3_1:11563314-11563333 None:intergenic 40.0%
GAGGCTATGATGAGGAAAAA+AGG + chr3_1:11563376-11563395 Msa0348740:CDS 40.0%
TGTTGACACCATTTGGTTAC+TGG - chr3_1:11564098-11564117 None:intergenic 40.0%
AAAGTGGGACAAGGAATCAT+GGG - chr3_1:11564262-11564281 None:intergenic 40.0%
AATGACAAGAAAGTGGGACA+AGG - chr3_1:11564271-11564290 None:intergenic 40.0%
TGTCCCACTTTCTTGTCATT+TGG + chr3_1:11564271-11564290 Msa0348740:three_prime_UTR 40.0%
!! GATTTTGAAGAGAGGAAGCA+AGG - chr3_1:11562960-11562979 None:intergenic 40.0%
!! GAAGAGGTGATTTTGAAGAG+AGG - chr3_1:11562968-11562987 None:intergenic 40.0%
! GATGCCATTTCTTGAAACCA+AGG - chr3_1:11563135-11563154 None:intergenic 40.0%
TAGTGTCTCTTAACATCCTC+AGG - chr3_1:11563285-11563304 None:intergenic 40.0%
GACACTATGAACACCTTCTA+AGG + chr3_1:11563298-11563317 Msa0348740:CDS 40.0%
! AACACCTTCTAAGGGATGTT+AGG + chr3_1:11563307-11563326 Msa0348740:CDS 40.0%
TATGCCTAACATCCCTTAGA+AGG - chr3_1:11563314-11563333 None:intergenic 40.0%
GAGGCTATGATGAGGAAAAA+AGG + chr3_1:11563376-11563395 Msa0348740:CDS 40.0%
TGTTGACACCATTTGGTTAC+TGG - chr3_1:11564098-11564117 None:intergenic 40.0%
AAAGTGGGACAAGGAATCAT+GGG - chr3_1:11564262-11564281 None:intergenic 40.0%
AATGACAAGAAAGTGGGACA+AGG - chr3_1:11564271-11564290 None:intergenic 40.0%
TGTCCCACTTTCTTGTCATT+TGG + chr3_1:11564271-11564290 Msa0348740:three_prime_UTR 40.0%
! AAGAAGAATTAGAGGGCGTG+TGG - chr3_1:11562923-11562942 None:intergenic 45.0%
TTGAAGAGAGGAAGCAAGGT+TGG - chr3_1:11562956-11562975 None:intergenic 45.0%
ATGGCATCAATGTCAGCATC+TGG + chr3_1:11563147-11563166 Msa0348740:CDS 45.0%
! CAATGTCAGCATCTGGTTCA+TGG + chr3_1:11563154-11563173 Msa0348740:CDS 45.0%
! ATCTGGTTCATGGAGTGCTA+AGG + chr3_1:11563164-11563183 Msa0348740:CDS 45.0%
ATAAAGACACACCTGACCGT+TGG + chr3_1:11563223-11563242 Msa0348740:CDS 45.0%
CTTGAGCAACATTGGACCAA+CGG - chr3_1:11563242-11563261 None:intergenic 45.0%
TCCAATGTTGCTCAAGCTGT+TGG + chr3_1:11563246-11563265 Msa0348740:CDS 45.0%
ACCAACAGCTTGAGCAACAT+TGG - chr3_1:11563250-11563269 None:intergenic 45.0%
AATGTTGCTCAAGCTGTTGG+TGG + chr3_1:11563249-11563268 Msa0348740:CDS 45.0%
CAACATTGGAGGCTATGATG+AGG + chr3_1:11563368-11563387 Msa0348740:CDS 45.0%
AACCTGCAAGTGAAAGCAAG+AGG - chr3_1:11564060-11564079 None:intergenic 45.0%
CATTTGGTTACTGGAGCTTC+AGG - chr3_1:11564089-11564108 None:intergenic 45.0%
CTGAAGCTCCAGTAACCAAA+TGG + chr3_1:11564087-11564106 Msa0348740:exon 45.0%
GAAAGTGGGACAAGGAATCA+TGG - chr3_1:11564263-11564282 None:intergenic 45.0%
! AAGAAGAATTAGAGGGCGTG+TGG - chr3_1:11562923-11562942 None:intergenic 45.0%
TTGAAGAGAGGAAGCAAGGT+TGG - chr3_1:11562956-11562975 None:intergenic 45.0%
ATGGCATCAATGTCAGCATC+TGG + chr3_1:11563147-11563166 Msa0348740:CDS 45.0%
! CAATGTCAGCATCTGGTTCA+TGG + chr3_1:11563154-11563173 Msa0348740:CDS 45.0%
! ATCTGGTTCATGGAGTGCTA+AGG + chr3_1:11563164-11563183 Msa0348740:CDS 45.0%
ATAAAGACACACCTGACCGT+TGG + chr3_1:11563223-11563242 Msa0348740:CDS 45.0%
CTTGAGCAACATTGGACCAA+CGG - chr3_1:11563242-11563261 None:intergenic 45.0%
TCCAATGTTGCTCAAGCTGT+TGG + chr3_1:11563246-11563265 Msa0348740:CDS 45.0%
ACCAACAGCTTGAGCAACAT+TGG - chr3_1:11563250-11563269 None:intergenic 45.0%
AATGTTGCTCAAGCTGTTGG+TGG + chr3_1:11563249-11563268 Msa0348740:CDS 45.0%
CAACATTGGAGGCTATGATG+AGG + chr3_1:11563368-11563387 Msa0348740:CDS 45.0%
AACCTGCAAGTGAAAGCAAG+AGG - chr3_1:11564060-11564079 None:intergenic 45.0%
CATTTGGTTACTGGAGCTTC+AGG - chr3_1:11564089-11564108 None:intergenic 45.0%
CTGAAGCTCCAGTAACCAAA+TGG + chr3_1:11564087-11564106 Msa0348740:exon 45.0%
GAAAGTGGGACAAGGAATCA+TGG - chr3_1:11564263-11564282 None:intergenic 45.0%
! AGAATTAGAGGGCGTGTGGA+AGG - chr3_1:11562919-11562938 None:intergenic 50.0%
!! TTGCTCAAGCTGTTGGTGGT+GGG + chr3_1:11563253-11563272 Msa0348740:CDS 50.0%
TGCCTCTTGCTTTCACTTGC+AGG + chr3_1:11564055-11564074 Msa0348740:intron 50.0%
! AGAATTAGAGGGCGTGTGGA+AGG - chr3_1:11562919-11562938 None:intergenic 50.0%
!! TTGCTCAAGCTGTTGGTGGT+GGG + chr3_1:11563253-11563272 Msa0348740:CDS 50.0%
TGCCTCTTGCTTTCACTTGC+AGG + chr3_1:11564055-11564074 Msa0348740:intron 50.0%
AGGTGTGGGGTAGAGCAAGT+TGG - chr3_1:11562899-11562918 None:intergenic 55.0%
GCAACATTGGACCAACGGTC+AGG - chr3_1:11563237-11563256 None:intergenic 55.0%
! GTTGCTCAAGCTGTTGGTGG+TGG + chr3_1:11563252-11563271 Msa0348740:CDS 55.0%
AGGTGTGGGGTAGAGCAAGT+TGG - chr3_1:11562899-11562918 None:intergenic 55.0%
GCAACATTGGACCAACGGTC+AGG - chr3_1:11563237-11563256 None:intergenic 55.0%
! GTTGCTCAAGCTGTTGGTGG+TGG + chr3_1:11563252-11563271 Msa0348740:CDS 55.0%
! AGAGGGCGTGTGGAAGGTGT+GGG - chr3_1:11562913-11562932 None:intergenic 60.0%
! TAGAGGGCGTGTGGAAGGTG+TGG - chr3_1:11562914-11562933 None:intergenic 60.0%
!! TGGTGGTGGGAAGACTCCTG+AGG + chr3_1:11563266-11563285 Msa0348740:CDS 60.0%
! AGAGGGCGTGTGGAAGGTGT+GGG - chr3_1:11562913-11562932 None:intergenic 60.0%
! TAGAGGGCGTGTGGAAGGTG+TGG - chr3_1:11562914-11562933 None:intergenic 60.0%
!! TGGTGGTGGGAAGACTCCTG+AGG + chr3_1:11563266-11563285 Msa0348740:CDS 60.0%
! GAGGGCGTGTGGAAGGTGTG+GGG - chr3_1:11562912-11562931 None:intergenic 65.0%
! GAGGGCGTGTGGAAGGTGTG+GGG - chr3_1:11562912-11562931 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 11562879 11564489 11562879 ID=Msa0348740;Name=Msa0348740
chr3_1 mRNA 11562879 11564489 11562879 ID=Msa0348740-mRNA-1;Parent=Msa0348740;Name=Msa0348740-mRNA-1;_AED=0.00;_eAED=0.00;_QI=268|1|1|1|1|1|2|388|91
chr3_1 exon 11562879 11563397 11562879 ID=Msa0348740-mRNA-1:exon:3018;Parent=Msa0348740-mRNA-1
chr3_1 exon 11564077 11564489 11564077 ID=Msa0348740-mRNA-1:exon:3019;Parent=Msa0348740-mRNA-1
chr3_1 five_prime_UTR 11562879 11563146 11562879 ID=Msa0348740-mRNA-1:five_prime_utr;Parent=Msa0348740-mRNA-1
chr3_1 CDS 11563147 11563397 11563147 ID=Msa0348740-mRNA-1:cds;Parent=Msa0348740-mRNA-1
chr3_1 CDS 11564077 11564101 11564077 ID=Msa0348740-mRNA-1:cds;Parent=Msa0348740-mRNA-1
chr3_1 three_prime_UTR 11564102 11564489 11564102 ID=Msa0348740-mRNA-1:three_prime_utr;Parent=Msa0348740-mRNA-1
Gene Sequence

>Msa0348740

ATGGCATCAATGTCAGCATCTGGTTCATGGAGTGCTAAGGACAATAAAGCATTTGAAAGGGCACTAGCTGTTTTTGATAAAGACACACCTGACCGTTGGTCCAATGTTGCTCAAGCTGTTGGTGGTGGGAAGACTCCTGAGGATGTTAAGAGACACTATGAACACCTTCTAAGGGATGTTAGGCATATTGAATTAGGTCATGTTGCATTCCCAAATTACAACAACATTGGAGGCTATGATGAGGAAAAAAGGTTGAGAAACCTGAAGCTCCAGTAA
Protein sequence

>Msa0348740

MASMSASGSWSAKDNKAFERALAVFDKDTPDRWSNVAQAVGGGKTPEDVKRHYEHLLRDVRHIELGHVAFPNYNNIGGYDEEKRLRNLKLQ*