AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0355650

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0355650 MtrunA17_Chr3g0092111 88.437 467 43 3 1 458 1 465 0.0 838
Msa0355650 MtrunA17_Chr4g0042481 44.153 496 204 21 1 456 1 463 4.05e-105 320
Msa0355650 MtrunA17_Chr6g0453321 43.164 512 220 18 1 458 1 495 9.22e-103 315
Msa0355650 MtrunA17_Chr7g0217581 42.885 513 211 19 1 458 98 583 1.85e-96 301
Msa0355650 MtrunA17_Chr8g0355261 34.576 483 235 20 3 457 9 438 2.56e-61 206
Msa0355650 MtrunA17_Chr5g0417561 39.402 368 116 14 100 456 90 361 5.32e-59 197
Msa0355650 MtrunA17_Chr5g0417551 35.012 417 153 15 52 456 59 369 1.39e-58 197
Msa0355650 MtrunA17_Chr5g0417601 35.680 412 161 17 49 456 68 379 2.29e-54 186
Msa0355650 MtrunA17_Chr7g0254161 34.222 450 196 17 1 412 3 390 8.49e-51 177
Msa0355650 MtrunA17_Chr5g0417571 49.286 140 64 2 59 193 70 207 5.02e-39 143
Msa0355650 MtrunA17_Chr6g0460731 36.162 271 128 9 76 333 42 280 3.81e-38 142
Msa0355650 MtrunA17_Chr6g0460721 37.743 257 131 9 118 350 78 329 1.56e-36 137
Msa0355650 MtrunA17_Chr2g0283811 78.333 60 13 0 123 182 41 100 6.41e-31 117
Msa0355650 MtrunA17_Chr8g0374111 56.322 87 34 2 129 215 29 111 4.05e-28 111
Msa0355650 MtrunA17_Chr3g0127201 60.000 75 30 0 108 182 6 80 9.80e-27 109
Msa0355650 MtrunA17_Chr2g0326001 69.697 66 19 1 118 182 28 93 4.23e-26 107
Msa0355650 MtrunA17_Chr8g0346551 69.492 59 18 0 124 182 19 77 5.85e-26 106
Msa0355650 MtrunA17_Chr1g0188851 49.515 103 46 3 131 233 45 141 1.42e-24 103
Msa0355650 MtrunA17_Chr8g0374251 67.797 59 19 0 129 187 68 126 2.84e-24 103
Msa0355650 MtrunA17_Chr4g0047001 76.923 52 12 0 131 182 57 108 3.72e-24 103
Msa0355650 MtrunA17_Chr4g0010151 59.091 66 27 0 117 182 70 135 5.43e-24 102
Msa0355650 MtrunA17_Chr4g0030051 67.692 65 20 1 118 181 29 93 5.59e-24 101
Msa0355650 MtrunA17_Chr1g0177251 66.129 62 20 1 131 191 14 75 6.55e-24 101
Msa0355650 MtrunA17_Chr3g0126201 73.077 52 14 0 131 182 53 104 8.71e-24 101
Msa0355650 MtrunA17_Chr3g0117601 66.129 62 20 1 119 179 50 111 1.11e-23 101
Msa0355650 MtrunA17_Chr2g0328021 70.370 54 16 0 129 182 62 115 1.74e-23 100
Msa0355650 MtrunA17_Chr4g0031411 59.722 72 28 1 117 187 63 134 2.40e-23 100
Msa0355650 MtrunA17_Chr2g0308011 75.510 49 12 0 129 177 37 85 5.38e-23 96.7
Msa0355650 MtrunA17_Chr8g0341671 64.516 62 22 0 129 190 20 81 5.76e-23 96.7
Msa0355650 MtrunA17_Chr8g0367161 50.602 83 35 1 100 182 9 85 9.25e-23 99.0
Msa0355650 MtrunA17_Chr2g0282511 75.510 49 12 0 129 177 45 93 1.12e-22 98.2
Msa0355650 MtrunA17_Chr2g0282051 75.000 52 12 1 131 182 48 98 2.19e-22 96.7
Msa0355650 MtrunA17_Chr4g0047761 68.519 54 17 0 129 182 72 125 2.68e-22 97.4
Msa0355650 MtrunA17_Chr2g0282441 72.000 50 14 0 131 180 27 76 2.69e-22 97.4
Msa0355650 MtrunA17_Chr7g0237921 57.576 66 28 0 117 182 64 129 3.28e-22 97.4
Msa0355650 MtrunA17_Chr4g0061761 67.925 53 17 0 127 179 38 90 3.41e-22 97.1
Msa0355650 MtrunA17_Chr7g0223311 72.549 51 14 0 131 181 79 129 4.21e-22 97.4
Msa0355650 MtrunA17_Chr5g0411951 63.636 55 20 0 125 179 39 93 8.97e-22 95.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0355650 AT3G47500.1 46.951 492 178 18 1 456 2 446 6.01e-109 330
Msa0355650 AT5G62430.1 44.297 377 112 12 86 456 12 296 5.77e-84 260
Msa0355650 AT5G39660.3 39.000 500 213 20 6 458 3 457 5.86e-84 266
Msa0355650 AT5G39660.1 39.000 500 213 20 6 458 3 457 5.86e-84 266
Msa0355650 AT5G39660.2 39.000 500 213 20 6 458 3 457 5.86e-84 266
Msa0355650 AT1G69570.1 34.375 480 208 18 1 457 1 396 2.97e-54 186
Msa0355650 AT1G26790.1 33.699 365 164 15 98 456 78 370 5.13e-44 158
Msa0355650 AT2G34140.1 56.989 93 40 0 90 182 19 111 4.61e-33 123
Msa0355650 AT1G29160.1 80.000 60 12 0 123 182 56 115 1.99e-32 122
Msa0355650 AT5G60200.1 49.091 110 51 2 125 229 51 160 4.11e-27 109
Msa0355650 AT3G61850.2 65.079 63 22 0 124 186 57 119 2.81e-25 105
Msa0355650 AT3G61850.3 65.079 63 22 0 124 186 57 119 2.81e-25 105
Msa0355650 AT3G50410.1 71.930 57 16 0 129 185 30 86 3.03e-25 104
Msa0355650 AT3G61850.1 65.079 63 22 0 124 186 69 131 3.07e-25 105
Msa0355650 AT2G28510.1 71.186 59 17 0 131 189 50 108 3.52e-25 105
Msa0355650 AT4G24060.1 62.121 66 25 0 117 182 41 106 3.62e-25 106
Msa0355650 AT3G61850.5 65.079 63 22 0 124 186 57 119 4.09e-25 105
Msa0355650 AT3G61850.4 65.079 63 22 0 124 186 69 131 4.77e-25 105
Msa0355650 AT3G45610.1 61.905 63 24 0 125 187 36 98 5.41e-25 103
Msa0355650 AT2G28810.2 65.574 61 21 0 120 180 68 128 1.59e-24 103
Msa0355650 AT1G51700.1 57.333 75 28 1 129 199 33 107 2.15e-24 100
Msa0355650 AT2G28810.1 65.574 61 21 0 120 180 85 145 2.33e-24 103
Msa0355650 AT1G28310.1 74.074 54 14 0 129 182 27 80 3.73e-24 102
Msa0355650 AT1G28310.2 74.074 54 14 0 129 182 51 104 6.09e-24 102
Msa0355650 AT4G00940.1 67.797 59 19 0 124 182 63 121 1.04e-23 101
Msa0355650 AT4G00940.3 67.797 59 19 0 124 182 63 121 1.04e-23 101
Msa0355650 AT4G00940.2 67.797 59 19 0 124 182 63 121 1.04e-23 101
Msa0355650 AT3G55370.1 55.714 70 31 0 125 194 72 141 1.06e-23 101
Msa0355650 AT1G21340.1 62.295 61 22 1 131 190 40 100 1.08e-23 100
Msa0355650 AT5G60850.1 62.121 66 25 0 117 182 39 104 1.11e-23 101
Msa0355650 AT3G21270.1 73.585 53 14 0 129 181 29 81 1.15e-23 98.6
Msa0355650 AT3G55370.2 55.714 70 31 0 125 194 72 141 1.25e-23 102
Msa0355650 AT1G47655.1 77.551 49 11 0 129 177 29 77 1.41e-23 98.6
Msa0355650 AT3G55370.3 55.714 70 31 0 125 194 117 186 1.69e-23 102
Msa0355650 AT2G37590.1 57.576 66 28 0 117 182 77 142 1.70e-23 101
Msa0355650 AT1G07640.1 71.698 53 15 0 129 181 21 73 1.73e-23 100
Msa0355650 AT1G07640.2 71.698 53 15 0 129 181 77 129 1.85e-23 101
Msa0355650 AT3G52440.1 68.421 57 18 0 131 187 27 83 2.38e-23 99.0
Msa0355650 AT5G66940.1 48.315 89 43 1 103 188 3 91 2.38e-23 98.6
Msa0355650 AT1G07640.3 71.698 53 15 0 129 181 85 137 2.50e-23 100
Msa0355650 AT3G52440.2 68.421 57 18 0 131 187 46 102 2.67e-23 99.4
Msa0355650 AT1G64620.1 63.934 61 22 0 117 177 37 97 2.94e-23 100
Msa0355650 AT2G46590.1 69.811 53 16 0 124 176 63 115 4.99e-23 100
Msa0355650 AT2G46590.2 69.811 53 16 0 124 176 75 127 5.38e-23 100
Msa0355650 AT5G65590.1 70.370 54 16 0 129 182 41 94 5.79e-23 99.4
Msa0355650 AT5G02460.1 59.091 66 27 0 117 182 83 148 1.52e-22 99.8
Msa0355650 AT4G21050.1 57.971 69 29 0 114 182 9 77 1.69e-21 92.8
Msa0355650 AT5G62940.1 73.469 49 13 0 131 179 75 123 1.72e-21 96.3
Msa0355650 AT4G38000.1 64.286 56 19 1 128 183 40 94 6.08e-21 92.0
Msa0355650 AT4G21040.1 63.462 52 19 0 131 182 27 78 1.34e-18 85.1
Msa0355650 AT4G21080.1 61.538 52 20 0 131 182 27 78 7.94e-18 83.2
Msa0355650 AT4G21030.1 59.091 44 18 0 131 174 23 66 1.01e-11 64.3

Find 104 sgRNAs with CRISPR-Local

Find 168 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGAATGTTCCTTGGTTTC+CGG 0.171508 3_1:+30419599 Msa0355650:CDS
ACACATCCATTTCTTTGTTT+TGG 0.252620 3_1:-30419747 None:intergenic
TCTCAATTCTTCGGAGAAAA+TGG 0.261398 3_1:+30419326 Msa0355650:CDS
GAATAGGGGTGGTATGATTA+AGG 0.274373 3_1:+30419860 Msa0355650:CDS
GCAAGGCGTGTCAAAGATAT+TGG 0.279470 3_1:+30419079 Msa0355650:CDS
TGTCACAGTTTCAAAGTCTA+TGG 0.284931 3_1:+30419398 Msa0355650:CDS
AAGCTGCAAAGAGTTCAATT+TGG 0.294104 3_1:+30419808 Msa0355650:CDS
GAACTCTTTGCAGCTTCATT+TGG 0.306321 3_1:-30419801 None:intergenic
TTCCCGAGTGTTGGAGATTT+CGG 0.311244 3_1:-30419654 None:intergenic
ACAAAGAAATGGATGTGTTT+TGG 0.315685 3_1:+30419752 Msa0355650:CDS
TAAGAATGAAGGTTTGAATA+GGG 0.329266 3_1:+30419845 Msa0355650:CDS
GCAAGAATTGTTTCTCCTAA+TGG 0.336437 3_1:+30419213 Msa0355650:CDS
GTGCTCCTTGGCCTTATACT+TGG 0.364975 3_1:+30419502 Msa0355650:CDS
TCATTACCACAGCAAAATAA+TGG 0.365655 3_1:+30419450 Msa0355650:CDS
AGCTGCAAAGAGTTCAATTT+GGG 0.368742 3_1:+30419809 Msa0355650:CDS
GCGCCGGAACTCCAAGTATA+AGG 0.369585 3_1:-30419513 None:intergenic
TTAAGAATGAAGGTTTGAAT+AGG 0.377391 3_1:+30419844 Msa0355650:CDS
TGAAGATGATGAGTACCATT+AGG 0.379458 3_1:-30419228 None:intergenic
GTGATTCTTCTGTTTGCAAA+TGG 0.382048 3_1:-30419725 None:intergenic
AATGGTAGAGTCCTCAGCTT+TGG 0.387317 3_1:+30419264 Msa0355650:CDS
GCCACAAACTTTGTGTCCTC+CGG 0.388135 3_1:+30419545 Msa0355650:CDS
AACCAAGGAACATTCCAAAA+AGG 0.400329 3_1:-30419594 None:intergenic
TGGCCTTATACTTGGAGTTC+CGG 0.405276 3_1:+30419510 Msa0355650:CDS
TTCTTGATCTCTACGGCATC+TGG 0.409446 3_1:-30418820 None:intergenic
TCTTGAGCGTTGTTGTTGTT+TGG 0.409763 3_1:-30418946 None:intergenic
GCGCTGTTGCAGCGTGGACA+CGG 0.414640 3_1:-30419000 None:intergenic
AGAAATGTTCCGGTCGGTGC+AGG 0.415448 3_1:+30419120 Msa0355650:CDS
GTCAAATGATCTCAATTCTT+CGG 0.423186 3_1:+30419317 Msa0355650:CDS
TCAAACCTCTCCAATGTTGA+TGG 0.423970 3_1:+30419917 Msa0355650:CDS
ATGGTAGAGTCCTCAGCTTT+GGG 0.430451 3_1:+30419265 Msa0355650:CDS
GAATCACCAAAACAAAGAAA+TGG 0.432133 3_1:+30419741 Msa0355650:CDS
TGTTTCGCCGTCGGCGGCCA+TGG 0.434041 3_1:+30417369 Msa0355650:CDS
TTCTTATTTAAGAGTTCTCA+TGG 0.441058 3_1:-30419974 None:intergenic
CATGTATGACTAGTGCTCCT+TGG 0.447113 3_1:+30419490 Msa0355650:CDS
CTAGACTTTGGAGTTGCCGC+GGG 0.452991 3_1:-30419630 None:intergenic
CGGAACCATGAGAAATGTTC+CGG 0.463308 3_1:+30419110 Msa0355650:CDS
ACACTTGGAATTAAGAATGA+AGG 0.464706 3_1:+30419834 Msa0355650:CDS
TTCCTTCTCCCTGCACCGAC+CGG 0.469734 3_1:-30419129 None:intergenic
ATCTCTACGGCATCTGGTGG+AGG 0.473653 3_1:-30418814 None:intergenic
CTCGAGTGTTTCCCGAGTGT+TGG 0.475061 3_1:-30419663 None:intergenic
CTTAACGACACTCGAAATAA+CGG 0.475197 3_1:+30419351 Msa0355650:CDS
GGAGATTTCGGACTAGACTT+TGG 0.480630 3_1:-30419642 None:intergenic
AGTGATCTAGCTAAAGCTGC+AGG 0.482293 3_1:-30419945 None:intergenic
TTCCGGTCGGTGCAGGGAGA+AGG 0.485518 3_1:+30419127 Msa0355650:CDS
CCCGGAGGACACAAAGTTTG+TGG 0.488796 3_1:-30419546 None:intergenic
AACAATTCAGCAGCAACATT+GGG 0.489579 3_1:+30418850 Msa0355650:CDS
TCTTCCTTCTTCTACATCCA+TGG 0.494055 3_1:-30417386 None:intergenic
GTTGTCGTTCTTGATCTCTA+CGG 0.497781 3_1:-30418827 None:intergenic
CCACAAACTTTGTGTCCTCC+GGG 0.498260 3_1:+30419546 Msa0355650:CDS
AGTTCAATTTGGGAAACACT+TGG 0.509045 3_1:+30419819 Msa0355650:CDS
TAGAGTCCTCAGCTTTGGGT+TGG 0.513593 3_1:+30419269 Msa0355650:CDS
CGCTGTTGCAGCGTGGACAC+GGG 0.514091 3_1:-30418999 None:intergenic
TGTCAAAGATATTGGACTGC+AGG 0.515747 3_1:+30419087 Msa0355650:CDS
GGACACGGGAGTAATTTGTC+TGG 0.517983 3_1:-30418985 None:intergenic
GATGTAGAAGAAGGAAGAGA+TGG 0.521727 3_1:+30417391 Msa0355650:CDS
CAACAATTCAGCAGCAACAT+TGG 0.525068 3_1:+30418849 Msa0355650:CDS
CTTCAAAATTTGAAAACCAA+TGG 0.530626 3_1:+30419246 Msa0355650:CDS
GAAGTTTCTTCATCTATGGA+AGG 0.537934 3_1:-30418892 None:intergenic
GACATTGAATCATAGATATG+TGG 0.538845 3_1:-30419297 None:intergenic
AAGAATGAAGGTTTGAATAG+GGG 0.550009 3_1:+30419846 Msa0355650:CDS
AAAGCTGAGGACTCTACCAT+TGG 0.551075 3_1:-30419262 None:intergenic
GGACACAAAGTTTGTGGCGA+GGG 0.556620 3_1:-30419540 None:intergenic
TCTACATCCATGGCCGCCGA+CGG 0.571064 3_1:-30417376 None:intergenic
TTGTGGCGAGGGAATTGCGC+CGG 0.571745 3_1:-30419529 None:intergenic
TAAAATGACATGGGAAATCC+CGG 0.575941 3_1:-30419564 None:intergenic
CAACACTCGGGAAACACTCG+AGG 0.576028 3_1:+30419664 Msa0355650:CDS
GAACTCCAAGTATAAGGCCA+AGG 0.577392 3_1:-30419507 None:intergenic
TCAAAGTCTATGGAGGAAAG+TGG 0.579037 3_1:+30419408 Msa0355650:CDS
CAAGGAGCACTAGTCATACA+TGG 0.583891 3_1:-30419489 None:intergenic
AGAAAATGTTTCGCCGTCGG+CGG 0.586336 3_1:+30417363 Msa0355650:CDS
GCAGGGTTAGCCATCAACAT+TGG 0.587131 3_1:-30419927 None:intergenic
GCGGCCATGGATGTAGAAGA+AGG 0.589032 3_1:+30417382 Msa0355650:CDS
AGGAGCAGGGTAAAATGACA+TGG 0.595195 3_1:-30419574 None:intergenic
AGTCCGAAATCTCCAACACT+CGG 0.595742 3_1:+30419651 Msa0355650:CDS
TTGATCTCTACGGCATCTGG+TGG 0.597414 3_1:-30418817 None:intergenic
ACTAGACTTTGGAGTTGCCG+CGG 0.599886 3_1:-30419631 None:intergenic
TGAAGAAGAAGAAATCGAGA+AGG 0.603186 3_1:+30417441 Msa0355650:CDS
GGGGTATGAGCCGGAAACCA+AGG 0.603372 3_1:-30419609 None:intergenic
ACCGACCGGAACATTTCTCA+TGG 0.607090 3_1:-30419115 None:intergenic
ATAAGACCTTCACCTTCCCC+AGG 0.607317 3_1:-30417328 None:intergenic
CAAAGATATTGGACTGCAGG+CGG 0.608252 3_1:+30419090 Msa0355650:CDS
AATGAAGGTTTGAATAGGGG+TGG 0.610382 3_1:+30419849 Msa0355650:CDS
GAAATGTTCCGGTCGGTGCA+GGG 0.612559 3_1:+30419121 Msa0355650:CDS
GTCCGAAATCTCCAACACTC+GGG 0.615508 3_1:+30419652 Msa0355650:CDS
CACAGTTTCAAAGTCTATGG+AGG 0.616952 3_1:+30419401 Msa0355650:CDS
GGAAGAAAATGTTTCGCCGT+CGG 0.618958 3_1:+30417360 Msa0355650:CDS
TGCATCAAGAGCCTCAGAGA+TGG 0.626336 3_1:-30419190 None:intergenic
GTTAGCCATCAACATTGGAG+AGG 0.627108 3_1:-30419922 None:intergenic
GTGATCTAGCTAAAGCTGCA+GGG 0.636623 3_1:-30419944 None:intergenic
CTAGTCATACATGGAACTTG+AGG 0.637687 3_1:-30419480 None:intergenic
ACATTGGAGAGGTTTGAACA+TGG 0.639471 3_1:-30419911 None:intergenic
TAGACTTTGGAGTTGCCGCG+GGG 0.652593 3_1:-30419629 None:intergenic
AGGACACAAAGTTTGTGGCG+AGG 0.653889 3_1:-30419541 None:intergenic
ACCATGAGAAATGTTCCGGT+CGG 0.654901 3_1:+30419114 Msa0355650:CDS
GAGCCTCAGAGATGGTGATG+TGG 0.657067 3_1:-30419182 None:intergenic
TGAAGGTCTTATGATTGCTG+CGG 0.668448 3_1:+30417339 Msa0355650:CDS
GATGATCCAACCCAAAGCTG+AGG 0.671324 3_1:-30419275 None:intergenic
AACACTCGGGAAACACTCGA+GGG 0.674943 3_1:+30419665 Msa0355650:CDS
GGAGCAGGGTAAAATGACAT+GGG 0.680900 3_1:-30419573 None:intergenic
AGACTTTGGAGTTGCCGCGG+GGG 0.684903 3_1:-30419628 None:intergenic
TCGCCACATCACCATCTCTG+AGG 0.699550 3_1:+30419179 Msa0355650:CDS
GTGTCCGCGCTGTTGCAGCG+TGG 0.703298 3_1:-30419006 None:intergenic
GTGTCCACGCTGCAACAGCG+CGG 0.758797 3_1:+30419002 Msa0355650:CDS
AATGACATGGGAAATCCCGG+AGG 0.775230 3_1:-30419561 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATAGAAAAATTTGTAAAAA+AGG - chr3_1:30417835-30417854 None:intergenic 10.0%
!!! TTTTTACAAATTTTTCTATA+TGG + chr3_1:30417835-30417854 Msa0355650:intron 10.0%
!! AATTAAATAAATTTGAGCAT+AGG + chr3_1:30417787-30417806 Msa0355650:intron 15.0%
!! ATTAAATAAATTTGAGCATA+GGG + chr3_1:30417788-30417807 Msa0355650:intron 15.0%
!!! TTTCAATTTTTATATGAAGT+TGG + chr3_1:30418065-30418084 Msa0355650:intron 15.0%
!!! TTGATTTATTTTTGATGTTT+TGG + chr3_1:30418109-30418128 Msa0355650:intron 15.0%
!!! ACTAAAGATTTTTATGAGTA+AGG + chr3_1:30417517-30417536 Msa0355650:intron 20.0%
!! TGTTTGTATGATCTTATTAT+AGG + chr3_1:30417724-30417743 Msa0355650:intron 20.0%
!! AAAAATTGAAAATTCCCTAA+GGG - chr3_1:30417891-30417910 None:intergenic 20.0%
!! TAAAAATTGAAAATTCCCTA+AGG - chr3_1:30417892-30417911 None:intergenic 20.0%
!!! ATTTTTATATGAAGTTGGTA+AGG + chr3_1:30418070-30418089 Msa0355650:intron 20.0%
!! GATTATAAACAAAAAGACTT+AGG - chr3_1:30418287-30418306 None:intergenic 20.0%
!! AATATATATTGCATCAATTG+AGG - chr3_1:30418357-30418376 None:intergenic 20.0%
!! ATATTGTCTCAAATAATTAG+CGG + chr3_1:30418672-30418691 Msa0355650:intron 20.0%
! ATTGAAAAAGTCAATACGAT+TGG + chr3_1:30417661-30417680 Msa0355650:intron 25.0%
! AAAATTGAAAATTCCCTAAG+GGG - chr3_1:30417890-30417909 None:intergenic 25.0%
!! TTGCAATTTTTACATGAAGT+TGG + chr3_1:30418031-30418050 Msa0355650:intron 25.0%
! TTTAGTAACTCATATGAGTT+AGG - chr3_1:30418416-30418435 None:intergenic 25.0%
! TTCAGATTTGGAAAAATGTT+TGG + chr3_1:30418597-30418616 Msa0355650:intron 25.0%
! ATGAAAACATTGTGTCAAAT+AGG + chr3_1:30418746-30418765 Msa0355650:intron 25.0%
! TGAAAACATTGTGTCAAATA+GGG + chr3_1:30418747-30418766 Msa0355650:intron 25.0%
!!! TTTTGAAGTTTCTTCATCTA+TGG - chr3_1:30418899-30418918 None:intergenic 25.0%
!! TGTATCACTTTGTTCATTTT+CGG - chr3_1:30418923-30418942 None:intergenic 25.0%
! CTTCAAAATTTGAAAACCAA+TGG + chr3_1:30419246-30419265 Msa0355650:CDS 25.0%
!! CATCAATTCTCAATGTTTTA+GGG - chr3_1:30419779-30419798 None:intergenic 25.0%
!! TCATCAATTCTCAATGTTTT+AGG - chr3_1:30419780-30419799 None:intergenic 25.0%
! TTAAGAATGAAGGTTTGAAT+AGG + chr3_1:30419844-30419863 Msa0355650:CDS 25.0%
! TAAGAATGAAGGTTTGAATA+GGG + chr3_1:30419845-30419864 Msa0355650:CDS 25.0%
! ACCCTGAAATTAAGCTTTTT+GGG + chr3_1:30417290-30417309 Msa0355650:CDS 30.0%
TCCCAAAAAGCTTAATTTCA+GGG - chr3_1:30417294-30417313 None:intergenic 30.0%
TTCCCAAAAAGCTTAATTTC+AGG - chr3_1:30417295-30417314 None:intergenic 30.0%
ATCGTGCATATTTGATATCA+CGG + chr3_1:30417560-30417579 Msa0355650:intron 30.0%
AACAAATAGATAGATACTCG+TGG - chr3_1:30417635-30417654 None:intergenic 30.0%
TAAATTTGAGCATAGGGTAA+TGG + chr3_1:30417794-30417813 Msa0355650:intron 30.0%
ACAAGAAGAACATCAAGAAA+AGG - chr3_1:30418502-30418521 None:intergenic 30.0%
AAATTATGCACTTATGCTCT+TGG + chr3_1:30418557-30418576 Msa0355650:intron 30.0%
TTAATGATGAACATGAGACA+CGG - chr3_1:30418720-30418739 None:intergenic 30.0%
GACATTGAATCATAGATATG+TGG - chr3_1:30419300-30419319 None:intergenic 30.0%
GTCAAATGATCTCAATTCTT+CGG + chr3_1:30419317-30419336 Msa0355650:CDS 30.0%
TCATTACCACAGCAAAATAA+TGG + chr3_1:30419450-30419469 Msa0355650:CDS 30.0%
!! ATGAAACCATTATTTTGCTG+TGG - chr3_1:30419459-30419478 None:intergenic 30.0%
GAATCACCAAAACAAAGAAA+TGG + chr3_1:30419741-30419760 Msa0355650:CDS 30.0%
!! ACACATCCATTTCTTTGTTT+TGG - chr3_1:30419750-30419769 None:intergenic 30.0%
! ACAAAGAAATGGATGTGTTT+TGG + chr3_1:30419752-30419771 Msa0355650:CDS 30.0%
ACACTTGGAATTAAGAATGA+AGG + chr3_1:30419834-30419853 Msa0355650:CDS 30.0%
AAGAATGAAGGTTTGAATAG+GGG + chr3_1:30419846-30419865 Msa0355650:CDS 30.0%
! GCTTTTCAATCAAAGAAAGA+TGG + chr3_1:30419882-30419901 Msa0355650:CDS 30.0%
! GACCCTGAAATTAAGCTTTT+TGG + chr3_1:30417289-30417308 Msa0355650:CDS 35.0%
! GGAAGAAGATTCTTTTTCCT+GGG + chr3_1:30417311-30417330 Msa0355650:CDS 35.0%
! GAAGAAGATTCTTTTTCCTG+GGG + chr3_1:30417312-30417331 Msa0355650:CDS 35.0%
TGAAGAAGAAGAAATCGAGA+AGG + chr3_1:30417441-30417460 Msa0355650:CDS 35.0%
!! TGTTCTGAAATTTACCCCTT+AGG + chr3_1:30417873-30417892 Msa0355650:intron 35.0%
!! GTTCTGAAATTTACCCCTTA+GGG + chr3_1:30417874-30417893 Msa0355650:intron 35.0%
TCAAAAATACTGAGACAGCA+GGG + chr3_1:30418188-30418207 Msa0355650:intron 35.0%
TTCTACTGTATAGTGCTCAT+AGG - chr3_1:30418250-30418269 None:intergenic 35.0%
! AACTATTGTATGTGTCACTG+TGG - chr3_1:30418638-30418657 None:intergenic 35.0%
AACAATTCAGCAGCAACATT+GGG + chr3_1:30418850-30418869 Msa0355650:CDS 35.0%
!!! TCATCTATGGAAGGTGTTTT+CGG - chr3_1:30418886-30418905 None:intergenic 35.0%
GAAGTTTCTTCATCTATGGA+AGG - chr3_1:30418895-30418914 None:intergenic 35.0%
! GCAAGAATTGTTTCTCCTAA+TGG + chr3_1:30419213-30419232 Msa0355650:CDS 35.0%
TGAAGATGATGAGTACCATT+AGG - chr3_1:30419231-30419250 None:intergenic 35.0%
TCTCAATTCTTCGGAGAAAA+TGG + chr3_1:30419326-30419345 Msa0355650:CDS 35.0%
CTTAACGACACTCGAAATAA+CGG + chr3_1:30419351-30419370 Msa0355650:CDS 35.0%
TGTCACAGTTTCAAAGTCTA+TGG + chr3_1:30419398-30419417 Msa0355650:CDS 35.0%
TAAAATGACATGGGAAATCC+CGG - chr3_1:30419567-30419586 None:intergenic 35.0%
AACCAAGGAACATTCCAAAA+AGG - chr3_1:30419597-30419616 None:intergenic 35.0%
GTGATTCTTCTGTTTGCAAA+TGG - chr3_1:30419728-30419747 None:intergenic 35.0%
AAGCTGCAAAGAGTTCAATT+TGG + chr3_1:30419808-30419827 Msa0355650:CDS 35.0%
AGCTGCAAAGAGTTCAATTT+GGG + chr3_1:30419809-30419828 Msa0355650:CDS 35.0%
AGTTCAATTTGGGAAACACT+TGG + chr3_1:30419819-30419838 Msa0355650:CDS 35.0%
! GGGAAGAAGATTCTTTTTCC+TGG + chr3_1:30417310-30417329 Msa0355650:CDS 40.0%
! AAGATTCTTTTTCCTGGGGA+AGG + chr3_1:30417316-30417335 Msa0355650:CDS 40.0%
! TGAAGGTCTTATGATTGCTG+CGG + chr3_1:30417339-30417358 Msa0355650:CDS 40.0%
TCTTCCTTCTTCTACATCCA+TGG - chr3_1:30417389-30417408 None:intergenic 40.0%
GATGTAGAAGAAGGAAGAGA+TGG + chr3_1:30417391-30417410 Msa0355650:CDS 40.0%
GTGCATATTTGATATCACGG+TGG + chr3_1:30417563-30417582 Msa0355650:intron 40.0%
!! TTGAGCATAGGGTAATGGAT+TGG + chr3_1:30417799-30417818 Msa0355650:intron 40.0%
GTCAAAAATACTGAGACAGC+AGG + chr3_1:30418187-30418206 Msa0355650:intron 40.0%
ACTGTATAGTGCTCATAGGA+AGG - chr3_1:30418246-30418265 None:intergenic 40.0%
ACTTATGCTCTTGGATATGC+AGG + chr3_1:30418566-30418585 Msa0355650:intron 40.0%
CAGGACTGATACTTCAGATT+TGG + chr3_1:30418585-30418604 Msa0355650:intron 40.0%
TCAGCATCTTTATCTGCTTC+AGG - chr3_1:30418775-30418794 None:intergenic 40.0%
GTTGTCGTTCTTGATCTCTA+CGG - chr3_1:30418830-30418849 None:intergenic 40.0%
CAACAATTCAGCAGCAACAT+TGG + chr3_1:30418849-30418868 Msa0355650:CDS 40.0%
!!! CATCTATGGAAGGTGTTTTC+GGG - chr3_1:30418885-30418904 None:intergenic 40.0%
! TCTTGAGCGTTGTTGTTGTT+TGG - chr3_1:30418949-30418968 None:intergenic 40.0%
TGTCAAAGATATTGGACTGC+AGG + chr3_1:30419087-30419106 Msa0355650:CDS 40.0%
CACAGTTTCAAAGTCTATGG+AGG + chr3_1:30419401-30419420 Msa0355650:CDS 40.0%
TCAAAGTCTATGGAGGAAAG+TGG + chr3_1:30419408-30419427 Msa0355650:CDS 40.0%
CTAGTCATACATGGAACTTG+AGG - chr3_1:30419483-30419502 None:intergenic 40.0%
! CATTTTACCCTGCTCCTTTT+TGG + chr3_1:30419580-30419599 Msa0355650:CDS 40.0%
GAACATTCCAAAAAGGAGCA+GGG - chr3_1:30419590-30419609 None:intergenic 40.0%
!!! CTCCTTTTTGGAATGTTCCT+TGG + chr3_1:30419592-30419611 Msa0355650:CDS 40.0%
TTGGAATGTTCCTTGGTTTC+CGG + chr3_1:30419599-30419618 Msa0355650:CDS 40.0%
GAACTCTTTGCAGCTTCATT+TGG - chr3_1:30419804-30419823 None:intergenic 40.0%
AATGAAGGTTTGAATAGGGG+TGG + chr3_1:30419849-30419868 Msa0355650:CDS 40.0%
GAATAGGGGTGGTATGATTA+AGG + chr3_1:30419860-30419879 Msa0355650:CDS 40.0%
ACATTGGAGAGGTTTGAACA+TGG - chr3_1:30419914-30419933 None:intergenic 40.0%
TCAAACCTCTCCAATGTTGA+TGG + chr3_1:30419917-30419936 Msa0355650:CDS 40.0%
GGAAGAAAATGTTTCGCCGT+CGG + chr3_1:30417360-30417379 Msa0355650:CDS 45.0%
TTCTTGATCTCTACGGCATC+TGG - chr3_1:30418823-30418842 None:intergenic 45.0%
GCAAGGCGTGTCAAAGATAT+TGG + chr3_1:30419079-30419098 Msa0355650:CDS 45.0%
CAAAGATATTGGACTGCAGG+CGG + chr3_1:30419090-30419109 Msa0355650:CDS 45.0%
CGGAACCATGAGAAATGTTC+CGG + chr3_1:30419110-30419129 Msa0355650:CDS 45.0%
ACCATGAGAAATGTTCCGGT+CGG + chr3_1:30419114-30419133 Msa0355650:CDS 45.0%
AAAGCTGAGGACTCTACCAT+TGG - chr3_1:30419265-30419284 None:intergenic 45.0%
AATGGTAGAGTCCTCAGCTT+TGG + chr3_1:30419264-30419283 Msa0355650:CDS 45.0%
ATGGTAGAGTCCTCAGCTTT+GGG + chr3_1:30419265-30419284 Msa0355650:CDS 45.0%
!! CAAGGAGCACTAGTCATACA+TGG - chr3_1:30419492-30419511 None:intergenic 45.0%
CATGTATGACTAGTGCTCCT+TGG + chr3_1:30419490-30419509 Msa0355650:CDS 45.0%
GAACTCCAAGTATAAGGCCA+AGG - chr3_1:30419510-30419529 None:intergenic 45.0%
!! TGGCCTTATACTTGGAGTTC+CGG + chr3_1:30419510-30419529 Msa0355650:CDS 45.0%
GGAGCAGGGTAAAATGACAT+GGG - chr3_1:30419576-30419595 None:intergenic 45.0%
AGGAGCAGGGTAAAATGACA+TGG - chr3_1:30419577-30419596 None:intergenic 45.0%
GGAACATTCCAAAAAGGAGC+AGG - chr3_1:30419591-30419610 None:intergenic 45.0%
! GGAGATTTCGGACTAGACTT+TGG - chr3_1:30419645-30419664 None:intergenic 45.0%
AGTCCGAAATCTCCAACACT+CGG + chr3_1:30419651-30419670 Msa0355650:CDS 45.0%
!! TTCCCGAGTGTTGGAGATTT+CGG - chr3_1:30419657-30419676 None:intergenic 45.0%
GTTAGCCATCAACATTGGAG+AGG - chr3_1:30419925-30419944 None:intergenic 45.0%
GTGATCTAGCTAAAGCTGCA+GGG - chr3_1:30419947-30419966 None:intergenic 45.0%
AGTGATCTAGCTAAAGCTGC+AGG - chr3_1:30419948-30419967 None:intergenic 45.0%
!!! TTTTTGATTAAAAAAAATTT+AGG + chr3_1:30417917-30417936 Msa0355650:intron 5.0%
! CTTTTTCCTGGGGAAGGTGA+AGG + chr3_1:30417322-30417341 Msa0355650:CDS 50.0%
ATAAGACCTTCACCTTCCCC+AGG - chr3_1:30417331-30417350 None:intergenic 50.0%
AGAAAATGTTTCGCCGTCGG+CGG + chr3_1:30417363-30417382 Msa0355650:CDS 50.0%
TTGATCTCTACGGCATCTGG+TGG - chr3_1:30418820-30418839 None:intergenic 50.0%
! GGACACGGGAGTAATTTGTC+TGG - chr3_1:30418988-30419007 None:intergenic 50.0%
!!! TCAGCCGCGCTATTTTTGCA+AGG + chr3_1:30419062-30419081 Msa0355650:CDS 50.0%
CACGCCTTGCAAAAATAGCG+CGG - chr3_1:30419069-30419088 None:intergenic 50.0%
ACCGACCGGAACATTTCTCA+TGG - chr3_1:30419118-30419137 None:intergenic 50.0%
TGCATCAAGAGCCTCAGAGA+TGG - chr3_1:30419193-30419212 None:intergenic 50.0%
!! TAGAGTCCTCAGCTTTGGGT+TGG + chr3_1:30419269-30419288 Msa0355650:CDS 50.0%
GATGATCCAACCCAAAGCTG+AGG - chr3_1:30419278-30419297 None:intergenic 50.0%
!! GTGCTCCTTGGCCTTATACT+TGG + chr3_1:30419502-30419521 Msa0355650:CDS 50.0%
GGACACAAAGTTTGTGGCGA+GGG - chr3_1:30419543-30419562 None:intergenic 50.0%
AGGACACAAAGTTTGTGGCG+AGG - chr3_1:30419544-30419563 None:intergenic 50.0%
GCCACAAACTTTGTGTCCTC+CGG + chr3_1:30419545-30419564 Msa0355650:CDS 50.0%
CCACAAACTTTGTGTCCTCC+GGG + chr3_1:30419546-30419565 Msa0355650:CDS 50.0%
AATGACATGGGAAATCCCGG+AGG - chr3_1:30419564-30419583 None:intergenic 50.0%
!! ACTAGACTTTGGAGTTGCCG+CGG - chr3_1:30419634-30419653 None:intergenic 50.0%
GTCCGAAATCTCCAACACTC+GGG + chr3_1:30419652-30419671 Msa0355650:CDS 50.0%
AACACTCGGGAAACACTCGA+GGG + chr3_1:30419665-30419684 Msa0355650:CDS 50.0%
!! GCAGGGTTAGCCATCAACAT+TGG - chr3_1:30419930-30419949 None:intergenic 50.0%
GCGGCCATGGATGTAGAAGA+AGG + chr3_1:30417382-30417401 Msa0355650:CDS 55.0%
ATCTCTACGGCATCTGGTGG+AGG - chr3_1:30418817-30418836 None:intergenic 55.0%
AGAAATGTTCCGGTCGGTGC+AGG + chr3_1:30419120-30419139 Msa0355650:CDS 55.0%
GAAATGTTCCGGTCGGTGCA+GGG + chr3_1:30419121-30419140 Msa0355650:CDS 55.0%
TCGCCACATCACCATCTCTG+AGG + chr3_1:30419179-30419198 Msa0355650:CDS 55.0%
! GAGCCTCAGAGATGGTGATG+TGG - chr3_1:30419185-30419204 None:intergenic 55.0%
GCGCCGGAACTCCAAGTATA+AGG - chr3_1:30419516-30419535 None:intergenic 55.0%
CCCGGAGGACACAAAGTTTG+TGG - chr3_1:30419549-30419568 None:intergenic 55.0%
!! TAGACTTTGGAGTTGCCGCG+GGG - chr3_1:30419632-30419651 None:intergenic 55.0%
!! CTAGACTTTGGAGTTGCCGC+GGG - chr3_1:30419633-30419652 None:intergenic 55.0%
CTCGAGTGTTTCCCGAGTGT+TGG - chr3_1:30419666-30419685 None:intergenic 55.0%
CAACACTCGGGAAACACTCG+AGG + chr3_1:30419664-30419683 Msa0355650:CDS 55.0%
TCTACATCCATGGCCGCCGA+CGG - chr3_1:30417379-30417398 None:intergenic 60.0%
! TTCCTTCTCCCTGCACCGAC+CGG - chr3_1:30419132-30419151 None:intergenic 60.0%
TTGTGGCGAGGGAATTGCGC+CGG - chr3_1:30419532-30419551 None:intergenic 60.0%
GGGGTATGAGCCGGAAACCA+AGG - chr3_1:30419612-30419631 None:intergenic 60.0%
!! AGACTTTGGAGTTGCCGCGG+GGG - chr3_1:30419631-30419650 None:intergenic 60.0%
CGCTGTTGCAGCGTGGACAC+GGG - chr3_1:30419002-30419021 None:intergenic 65.0%
GCGCTGTTGCAGCGTGGACA+CGG - chr3_1:30419003-30419022 None:intergenic 65.0%
GTGTCCACGCTGCAACAGCG+CGG + chr3_1:30419002-30419021 Msa0355650:CDS 65.0%
TTCCGGTCGGTGCAGGGAGA+AGG + chr3_1:30419127-30419146 Msa0355650:CDS 65.0%
GTTTCCGGCTCATACCCCCG+CGG + chr3_1:30419614-30419633 Msa0355650:CDS 65.0%
TGTTTCGCCGTCGGCGGCCA+TGG + chr3_1:30417369-30417388 Msa0355650:CDS 70.0%
GTGTCCGCGCTGTTGCAGCG+TGG - chr3_1:30419009-30419028 None:intergenic 70.0%
! GTTGCCGCGGGGGTATGAGC+CGG - chr3_1:30419621-30419640 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 30417274 30419989 30417274 ID=Msa0355650;Name=Msa0355650
chr3_1 mRNA 30417274 30419989 30417274 ID=Msa0355650-mRNA-1;Parent=Msa0355650;Name=Msa0355650-mRNA-1;_AED=0.01;_eAED=0.02;_QI=0|0|0|1|1|1|2|0|458
chr3_1 exon 30417274 30417462 30417274 ID=Msa0355650-mRNA-1:exon:6278;Parent=Msa0355650-mRNA-1
chr3_1 exon 30418802 30419989 30418802 ID=Msa0355650-mRNA-1:exon:6279;Parent=Msa0355650-mRNA-1
chr3_1 CDS 30417274 30417462 30417274 ID=Msa0355650-mRNA-1:cds;Parent=Msa0355650-mRNA-1
chr3_1 CDS 30418802 30419989 30418802 ID=Msa0355650-mRNA-1:cds;Parent=Msa0355650-mRNA-1
Gene Sequence

>Msa0355650

ATGATTGAAACTAAGGACCCTGAAATTAAGCTTTTTGGGAAGAAGATTCTTTTTCCTGGGGAAGGTGAAGGTCTTATGATTGCTGCGGAAGAAAATGTTTCGCCGTCGGCGGCCATGGATGTAGAAGAAGGAAGAGATGGATATTATAGTGAGACTGAGAATGAAGATGAAGAAGAAGAAATCGAGAAGAAAGAAGCTGATCCTCCACCAGATGCCGTAGAGATCAAGAACGACAACAACAATTCAGCAGCAACATTGGGTATTCAGAACCCGAAAACACCTTCCATAGATGAAGAAACTTCAAAATCCGAAAATGAACAAAGTGATACAACACCAAACAACAACAACGCTCAAGAGAAAACACTAAAGAAACCAGACAAATTACTCCCGTGTCCACGCTGCAACAGCGCGGACACAAAATTCTGTTACTACAATAATTACAACGTCAATCAGCCGCGCTATTTTTGCAAGGCGTGTCAAAGATATTGGACTGCAGGCGGAACCATGAGAAATGTTCCGGTCGGTGCAGGGAGAAGGAAAAATAAGAACAACTCTTCGTCACATTATCGCCACATCACCATCTCTGAGGCTCTTGATGCAGCAAGAATTGTTTCTCCTAATGGTACTCATCATCTTCAAAATTTGAAAACCAATGGTAGAGTCCTCAGCTTTGGGTTGGATCATCCACATATCTATGATTCAATGTCAAATGATCTCAATTCTTCGGAGAAAATGGTTCTTAACGACACTCGAAATAACGGTGATCGTTTTTCGAGTGCATCTTCTGTCACAGTTTCAAAGTCTATGGAGGAAAGTGGTAAAAACATGTCACAAGAATCATTACCACAGCAAAATAATGGTTTCATTCCTCAAGTTCCATGTATGACTAGTGCTCCTTGGCCTTATACTTGGAGTTCCGGCGCAATTCCCTCGCCACAAACTTTGTGTCCTCCGGGATTTCCCATGTCATTTTACCCTGCTCCTTTTTGGAATGTTCCTTGGTTTCCGGCTCATACCCCCGCGGCAACTCCAAAGTCTAGTCCGAAATCTCCAACACTCGGGAAACACTCGAGGGATGACGAAAACAACAATGACGAAATTGCTAAACAAGACCATTTGCAAACAGAAGAATCACCAAAACAAAGAAATGGATGTGTTTTGGTCCCTAAAACATTGAGAATTGATGATCCAAATGAAGCTGCAAAGAGTTCAATTTGGGAAACACTTGGAATTAAGAATGAAGGTTTGAATAGGGGTGGTATGATTAAGGCTTTTCAATCAAAGAAAGATGGTAAAAACCATGTTCAAACCTCTCCAATGTTGATGGCTAACCCTGCAGCTTTAGCTAGATCACTTAATTTCCATGAGAACTCTTAA
Protein sequence

>Msa0355650

MIETKDPEIKLFGKKILFPGEGEGLMIAAEENVSPSAAMDVEEGRDGYYSETENEDEEEEIEKKEADPPPDAVEIKNDNNNSAATLGIQNPKTPSIDEETSKSENEQSDTTPNNNNAQEKTLKKPDKLLPCPRCNSADTKFCYYNNYNVNQPRYFCKACQRYWTAGGTMRNVPVGAGRRKNKNNSSSHYRHITISEALDAARIVSPNGTHHLQNLKTNGRVLSFGLDHPHIYDSMSNDLNSSEKMVLNDTRNNGDRFSSASSVTVSKSMEESGKNMSQESLPQQNNGFIPQVPCMTSAPWPYTWSSGAIPSPQTLCPPGFPMSFYPAPFWNVPWFPAHTPAATPKSSPKSPTLGKHSRDDENNNDEIAKQDHLQTEESPKQRNGCVLVPKTLRIDDPNEAAKSSIWETLGIKNEGLNRGGMIKAFQSKKDGKNHVQTSPMLMANPAALARSLNFHENS*