AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0356040


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0356040 MtrunA17_Chr3g0125801 92.982 57 4 0 3 59 8 64 1.75e-33 115
Msa0356040 MtrunA17_Chr7g0254261 70.370 54 16 0 6 59 84 137 8.91e-24 90.9
Msa0356040 MtrunA17_Chr4g0042501 74.074 54 14 0 6 59 100 153 3.29e-23 89.7
Msa0356040 MtrunA17_Chr6g0480581 74.510 51 13 0 9 59 2 52 5.27e-23 87.0
Msa0356040 MtrunA17_Chr5g0417621 72.222 54 15 0 6 59 129 182 7.50e-23 89.0
Msa0356040 MtrunA17_Chr6g0460671 61.818 55 21 0 5 59 93 147 1.00e-20 82.4
Msa0356040 MtrunA17_Chr6g0460661 66.667 48 16 0 12 59 17 64 2.09e-20 78.6
Msa0356040 MtrunA17_Chr4g0008551 60.417 48 19 0 12 59 39 86 1.54e-14 65.5
Msa0356040 MtrunA17_Chr5g0401731 56.250 48 21 0 12 59 16 63 3.96e-14 64.3
Msa0356040 MtrunA17_Chr4g0053431 56.250 48 21 0 12 59 6 53 1.06e-13 62.8
Msa0356040 MtrunA17_Chr3g0104501 58.333 48 20 0 12 59 134 181 1.25e-13 61.6
Msa0356040 MtrunA17_Chr5g0429661 58.333 48 20 0 12 59 123 170 1.72e-13 62.4
Msa0356040 MtrunA17_Chr5g0437321 54.167 48 22 0 12 59 20 67 3.35e-13 61.6
Msa0356040 MtrunA17_Chr3g0133601 56.250 48 21 0 12 59 38 85 1.08e-12 59.7
Msa0356040 MtrunA17_Chr6g0454921 54.167 48 22 0 12 59 16 63 1.17e-12 59.3
Msa0356040 MtrunA17_Chr4g0058991 56.250 48 21 0 12 59 210 257 8.67e-12 57.8
Msa0356040 MtrunA17_Chr7g0233161 52.083 48 23 0 12 59 300 347 3.66e-11 55.8
Msa0356040 MtrunA17_Chr3g0139671 50.000 48 24 0 12 59 86 133 5.37e-11 55.5
Msa0356040 MtrunA17_Chr3g0117511 52.083 48 23 0 12 59 184 231 6.01e-11 55.5
Msa0356040 MtrunA17_Chr5g0397841 52.174 46 22 0 14 59 113 158 8.73e-11 55.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0356040 AT1G73410.2 78.431 51 11 0 9 59 4 54 1.36e-24 90.5
Msa0356040 AT1G73410.1 78.431 51 11 0 9 59 4 54 2.55e-24 90.9
Msa0356040 AT1G17950.1 76.471 51 12 0 9 59 3 53 6.12e-24 89.7
Msa0356040 AT1G69560.1 76.923 52 12 0 8 59 104 155 1.00e-22 88.2
Msa0356040 AT1G69560.2 76.923 52 12 0 8 59 120 171 1.22e-22 87.8
Msa0356040 AT1G26780.1 81.250 48 9 0 12 59 99 146 2.15e-22 86.3
Msa0356040 AT4G33450.1 71.154 52 15 0 8 59 16 67 4.60e-22 85.1
Msa0356040 AT1G26780.2 81.250 48 9 0 12 59 99 146 5.41e-22 86.3
Msa0356040 AT5G17800.1 67.273 55 18 0 5 59 87 141 9.33e-22 85.5
Msa0356040 AT3G29020.1 64.815 54 19 0 6 59 60 113 1.67e-21 83.2
Msa0356040 AT3G29020.2 64.815 54 19 0 6 59 62 115 5.37e-21 83.2
Msa0356040 AT5G67300.1 56.250 48 21 0 12 59 7 54 1.48e-13 62.8
Msa0356040 AT5G39700.1 50.000 58 29 0 2 59 47 104 1.92e-13 61.2
Msa0356040 AT3G50060.1 54.167 48 22 0 12 59 7 54 2.29e-13 62.4
Msa0356040 AT5G40360.1 54.167 48 22 0 12 59 159 206 1.32e-12 60.5
Msa0356040 AT3G27785.1 54.167 48 22 0 12 59 190 237 1.76e-12 60.1
Msa0356040 AT2G39880.1 54.717 53 24 0 7 59 46 98 3.92e-12 59.3
Msa0356040 AT5G58850.1 50.000 54 27 0 6 59 100 153 5.14e-12 58.9
Msa0356040 AT3G09230.1 54.167 48 22 0 12 59 56 103 5.45e-12 58.9
Msa0356040 AT5G11050.1 50.943 53 26 0 7 59 101 153 6.69e-12 58.5
Msa0356040 AT2G23290.1 52.083 48 23 0 12 59 14 61 6.76e-12 58.2
Msa0356040 AT4G37260.1 52.083 48 23 0 12 59 14 61 9.65e-12 57.8
Msa0356040 AT3G09370.1 54.167 48 22 0 12 59 131 178 1.27e-11 57.8
Msa0356040 AT3G09370.2 54.167 48 22 0 12 59 136 183 1.27e-11 57.8
Msa0356040 AT3G09370.3 54.167 48 22 0 12 59 144 191 1.29e-11 57.8
Msa0356040 AT5G11510.2 52.083 48 23 0 12 59 82 129 1.88e-11 57.4
Msa0356040 AT5G11510.3 52.083 48 23 0 12 59 82 129 1.94e-11 57.4
Msa0356040 AT5G11510.1 52.083 48 23 0 12 59 82 129 1.94e-11 57.4
Msa0356040 AT5G11510.4 52.083 48 23 0 12 59 82 129 1.94e-11 57.4
Msa0356040 AT5G11510.5 52.083 48 23 0 12 59 82 129 1.97e-11 57.0
Msa0356040 AT3G55730.1 54.167 48 22 0 12 59 57 104 4.03e-11 56.2
Msa0356040 AT4G18770.1 54.167 48 22 0 12 59 218 265 5.33e-11 55.8

Find 34 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCATTGAAATTTCTTATT+TGG 0.138946 3_1:+31896484 None:intergenic
ACAACATTATCAGTTCTTTC+AGG 0.182418 3_1:+31896713 None:intergenic
AACAATATGGTGCTCAAAAT+TGG 0.232647 3_1:-31897135 Msa0356040:CDS
TTCCCTTACACGACGAGAAT+TGG 0.299194 3_1:-31896586 Msa0356040:three_prime_UTR
CCTCAACTTGTTGAACAATA+TGG 0.300197 3_1:-31897148 Msa0356040:CDS
AAAGGTCTCTTGTTAATTCA+TGG 0.340427 3_1:+31896815 None:intergenic
CAAGAGGCAAGAGAACTTAA+AGG 0.364207 3_1:+31896532 None:intergenic
ACTTCTCTTGCCACATAACT+TGG 0.368979 3_1:+31896643 None:intergenic
CTTTAAGTTCTCTTGCCTCT+TGG 0.373629 3_1:-31896531 Msa0356040:three_prime_UTR
ATCGAATTCACATAAACAAA+TGG 0.389392 3_1:-31896757 Msa0356040:three_prime_UTR
GAATCATAGGCATGTTATAA+TGG 0.391053 3_1:-31896687 Msa0356040:three_prime_UTR
TATCCAATTCTCGTCGTGTA+AGG 0.396192 3_1:+31896583 None:intergenic
TCTTCTTCCTCTTCTGTGAA+AGG 0.428456 3_1:+31896797 None:intergenic
CAACATTATCAGTTCTTTCA+GGG 0.435680 3_1:+31896714 None:intergenic
ATAATGTTGTCAAGAATCAT+AGG 0.447489 3_1:-31896700 Msa0356040:three_prime_UTR
TAGGGAAACGTTGTAGGTTG+AGG 0.463106 3_1:-31896856 Msa0356040:CDS
AACTTGTAGGGAAACGTTGT+AGG 0.477303 3_1:-31896862 Msa0356040:intron
TCGAATTCACATAAACAAAT+GGG 0.494256 3_1:-31896756 Msa0356040:three_prime_UTR
CAAGAGACCTTTCACAGAAG+AGG 0.503737 3_1:-31896804 Msa0356040:three_prime_UTR
AGAGTTGTCCATGTGGTCAC+TGG 0.512701 3_1:-31897195 Msa0356040:CDS
ACGAGAATTGGATATGAGAA+TGG 0.523637 3_1:-31896574 Msa0356040:three_prime_UTR
TATAATGGCTACAAAACAAA+GGG 0.531749 3_1:-31896672 Msa0356040:three_prime_UTR
GTGACCACATGGACAACTCT+TGG 0.543251 3_1:+31897198 None:intergenic
GTGTAAGGGAAATTAAGTAT+AGG 0.547906 3_1:+31896598 None:intergenic
ATCCAATTCTCGTCGTGTAA+GGG 0.553745 3_1:+31896584 None:intergenic
CCATATTGTTCAACAAGTTG+AGG 0.564383 3_1:+31897148 None:intergenic
AGGGAACAATCCAAGTTATG+TGG 0.586173 3_1:-31896653 Msa0356040:three_prime_UTR
TTATAATGGCTACAAAACAA+AGG 0.590289 3_1:-31896673 Msa0356040:three_prime_UTR
GGAAACGTTGTAGGTTGAGG+TGG 0.592608 3_1:-31896853 Msa0356040:CDS
GCGGGTCGCCAGTGACCACA+TGG 0.607822 3_1:+31897187 None:intergenic
ACTTCCATAATCCATATGGA+GGG 0.628080 3_1:-31897244 None:intergenic
CACAAAAGTAGAATTCCAAG+AGG 0.669761 3_1:+31896516 None:intergenic
GGAAGAAGAGAGACTACTTG+CGG 0.685866 3_1:-31896783 Msa0356040:three_prime_UTR
GATACCAAGAGTTGTCCATG+TGG 0.733953 3_1:-31897202 Msa0356040:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGAATTTGAAAATTAAATA+AGG + chr3_1:31896703-31896722 None:intergenic 10.0%
!! ATGAATTTGAAAATTAAATA+AGG + chr3_1:31896703-31896722 None:intergenic 10.0%
!! AATTTGAAAATTAAATAAGG+AGG + chr3_1:31896700-31896719 None:intergenic 15.0%
!! AATTTGAAAATTAAATAAGG+AGG + chr3_1:31896700-31896719 None:intergenic 15.0%
!! AATATTACATAATACATACC+TGG + chr3_1:31896645-31896664 None:intergenic 20.0%
!!! ATTTTTTCCTTTTAACTTGT+AGG - chr3_1:31896849-31896868 Msa0356040:CDS 20.0%
!!! TTTTTTCCTTTTAACTTGTA+GGG - chr3_1:31896850-31896869 Msa0356040:CDS 20.0%
!! AATATTACATAATACATACC+TGG + chr3_1:31896645-31896664 None:intergenic 20.0%
!!! ATTTTTTCCTTTTAACTTGT+AGG - chr3_1:31896849-31896868 Msa0356040:CDS 20.0%
!!! TTTTTTCCTTTTAACTTGTA+GGG - chr3_1:31896850-31896869 Msa0356040:CDS 20.0%
! ATCGAATTCACATAAACAAA+TGG - chr3_1:31896967-31896986 Msa0356040:intron 25.0%
! TCGAATTCACATAAACAAAT+GGG - chr3_1:31896968-31896987 Msa0356040:intron 25.0%
! ATAATGTTGTCAAGAATCAT+AGG - chr3_1:31897024-31897043 Msa0356040:intron 25.0%
! TTATAATGGCTACAAAACAA+AGG - chr3_1:31897051-31897070 Msa0356040:intron 25.0%
! TATAATGGCTACAAAACAAA+GGG - chr3_1:31897052-31897071 Msa0356040:intron 25.0%
! ATCGAATTCACATAAACAAA+TGG - chr3_1:31896967-31896986 Msa0356040:intron 25.0%
! TCGAATTCACATAAACAAAT+GGG - chr3_1:31896968-31896987 Msa0356040:intron 25.0%
! ATAATGTTGTCAAGAATCAT+AGG - chr3_1:31897024-31897043 Msa0356040:intron 25.0%
! TTATAATGGCTACAAAACAA+AGG - chr3_1:31897051-31897070 Msa0356040:intron 25.0%
! TATAATGGCTACAAAACAAA+GGG - chr3_1:31897052-31897071 Msa0356040:intron 25.0%
AACAATATGGTGCTCAAAAT+TGG - chr3_1:31896589-31896608 Msa0356040:three_prime_UTR 30.0%
TCAATTGCTGAAAAACTTCA+AGG - chr3_1:31896615-31896634 Msa0356040:three_prime_UTR 30.0%
AAAGGTCTCTTGTTAATTCA+TGG + chr3_1:31896912-31896931 None:intergenic 30.0%
CAACATTATCAGTTCTTTCA+GGG + chr3_1:31897013-31897032 None:intergenic 30.0%
ACAACATTATCAGTTCTTTC+AGG + chr3_1:31897014-31897033 None:intergenic 30.0%
GAATCATAGGCATGTTATAA+TGG - chr3_1:31897037-31897056 Msa0356040:intron 30.0%
GTGTAAGGGAAATTAAGTAT+AGG + chr3_1:31897129-31897148 None:intergenic 30.0%
AACAATATGGTGCTCAAAAT+TGG - chr3_1:31896589-31896608 Msa0356040:three_prime_UTR 30.0%
TCAATTGCTGAAAAACTTCA+AGG - chr3_1:31896615-31896634 Msa0356040:three_prime_UTR 30.0%
AAAGGTCTCTTGTTAATTCA+TGG + chr3_1:31896912-31896931 None:intergenic 30.0%
CAACATTATCAGTTCTTTCA+GGG + chr3_1:31897013-31897032 None:intergenic 30.0%
ACAACATTATCAGTTCTTTC+AGG + chr3_1:31897014-31897033 None:intergenic 30.0%
GAATCATAGGCATGTTATAA+TGG - chr3_1:31897037-31897056 Msa0356040:intron 30.0%
GTGTAAGGGAAATTAAGTAT+AGG + chr3_1:31897129-31897148 None:intergenic 30.0%
! AGGAAGTTTTTCATCTTCAG+CGG + chr3_1:31896559-31896578 None:intergenic 35.0%
CCATATTGTTCAACAAGTTG+AGG + chr3_1:31896579-31896598 None:intergenic 35.0%
!! CCTCAACTTGTTGAACAATA+TGG - chr3_1:31896576-31896595 Msa0356040:three_prime_UTR 35.0%
ACGTTTCCCTACAAGTTAAA+AGG + chr3_1:31896859-31896878 None:intergenic 35.0%
ACGAGAATTGGATATGAGAA+TGG - chr3_1:31897150-31897169 Msa0356040:CDS 35.0%
CACAAAAGTAGAATTCCAAG+AGG + chr3_1:31897211-31897230 None:intergenic 35.0%
! AGGAAGTTTTTCATCTTCAG+CGG + chr3_1:31896559-31896578 None:intergenic 35.0%
CCATATTGTTCAACAAGTTG+AGG + chr3_1:31896579-31896598 None:intergenic 35.0%
!! CCTCAACTTGTTGAACAATA+TGG - chr3_1:31896576-31896595 Msa0356040:three_prime_UTR 35.0%
ACGTTTCCCTACAAGTTAAA+AGG + chr3_1:31896859-31896878 None:intergenic 35.0%
ACGAGAATTGGATATGAGAA+TGG - chr3_1:31897150-31897169 Msa0356040:CDS 35.0%
CACAAAAGTAGAATTCCAAG+AGG + chr3_1:31897211-31897230 None:intergenic 35.0%
! GGAAGTTTTTCATCTTCAGC+GGG + chr3_1:31896558-31896577 None:intergenic 40.0%
GAAAAACTTCAAGGAAGACC+AGG - chr3_1:31896624-31896643 Msa0356040:three_prime_UTR 40.0%
AACTTGTAGGGAAACGTTGT+AGG - chr3_1:31896862-31896881 Msa0356040:intron 40.0%
TCTTCTTCCTCTTCTGTGAA+AGG + chr3_1:31896930-31896949 None:intergenic 40.0%
AGGGAACAATCCAAGTTATG+TGG - chr3_1:31897071-31897090 Msa0356040:intron 40.0%
ACTTCTCTTGCCACATAACT+TGG + chr3_1:31897084-31897103 None:intergenic 40.0%
ATCCAATTCTCGTCGTGTAA+GGG + chr3_1:31897143-31897162 None:intergenic 40.0%
TATCCAATTCTCGTCGTGTA+AGG + chr3_1:31897144-31897163 None:intergenic 40.0%
CAAGAGGCAAGAGAACTTAA+AGG + chr3_1:31897195-31897214 None:intergenic 40.0%
! CTTTAAGTTCTCTTGCCTCT+TGG - chr3_1:31897193-31897212 Msa0356040:CDS 40.0%
! GGAAGTTTTTCATCTTCAGC+GGG + chr3_1:31896558-31896577 None:intergenic 40.0%
GAAAAACTTCAAGGAAGACC+AGG - chr3_1:31896624-31896643 Msa0356040:three_prime_UTR 40.0%
AACTTGTAGGGAAACGTTGT+AGG - chr3_1:31896862-31896881 Msa0356040:intron 40.0%
TCTTCTTCCTCTTCTGTGAA+AGG + chr3_1:31896930-31896949 None:intergenic 40.0%
AGGGAACAATCCAAGTTATG+TGG - chr3_1:31897071-31897090 Msa0356040:intron 40.0%
ACTTCTCTTGCCACATAACT+TGG + chr3_1:31897084-31897103 None:intergenic 40.0%
ATCCAATTCTCGTCGTGTAA+GGG + chr3_1:31897143-31897162 None:intergenic 40.0%
TATCCAATTCTCGTCGTGTA+AGG + chr3_1:31897144-31897163 None:intergenic 40.0%
CAAGAGGCAAGAGAACTTAA+AGG + chr3_1:31897195-31897214 None:intergenic 40.0%
! CTTTAAGTTCTCTTGCCTCT+TGG - chr3_1:31897193-31897212 Msa0356040:CDS 40.0%
GATACCAAGAGTTGTCCATG+TGG - chr3_1:31896522-31896541 Msa0356040:three_prime_UTR 45.0%
TAGGGAAACGTTGTAGGTTG+AGG - chr3_1:31896868-31896887 Msa0356040:intron 45.0%
CAAGAGACCTTTCACAGAAG+AGG - chr3_1:31896920-31896939 Msa0356040:intron 45.0%
GGAAGAAGAGAGACTACTTG+CGG - chr3_1:31896941-31896960 Msa0356040:intron 45.0%
TTCCCTTACACGACGAGAAT+TGG - chr3_1:31897138-31897157 Msa0356040:CDS 45.0%
GATACCAAGAGTTGTCCATG+TGG - chr3_1:31896522-31896541 Msa0356040:three_prime_UTR 45.0%
TAGGGAAACGTTGTAGGTTG+AGG - chr3_1:31896868-31896887 Msa0356040:intron 45.0%
CAAGAGACCTTTCACAGAAG+AGG - chr3_1:31896920-31896939 Msa0356040:intron 45.0%
GGAAGAAGAGAGACTACTTG+CGG - chr3_1:31896941-31896960 Msa0356040:intron 45.0%
TTCCCTTACACGACGAGAAT+TGG - chr3_1:31897138-31897157 Msa0356040:CDS 45.0%
!! TATTATGTAATATTAATTAA+AGG - chr3_1:31896651-31896670 Msa0356040:three_prime_UTR 5.0%
!! TATTATGTAATATTAATTAA+AGG - chr3_1:31896651-31896670 Msa0356040:three_prime_UTR 5.0%
GTGACCACATGGACAACTCT+TGG + chr3_1:31896529-31896548 None:intergenic 50.0%
AGAGTTGTCCATGTGGTCAC+TGG - chr3_1:31896529-31896548 Msa0356040:three_prime_UTR 50.0%
! GGAAACGTTGTAGGTTGAGG+TGG - chr3_1:31896871-31896890 Msa0356040:intron 50.0%
GTGACCACATGGACAACTCT+TGG + chr3_1:31896529-31896548 None:intergenic 50.0%
AGAGTTGTCCATGTGGTCAC+TGG - chr3_1:31896529-31896548 Msa0356040:three_prime_UTR 50.0%
! GGAAACGTTGTAGGTTGAGG+TGG - chr3_1:31896871-31896890 Msa0356040:intron 50.0%
GCGGGTCGCCAGTGACCACA+TGG + chr3_1:31896540-31896559 None:intergenic 70.0%
GCGGGTCGCCAGTGACCACA+TGG + chr3_1:31896540-31896559 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 31896495 31897251 31896495 ID=Msa0356040;Name=Msa0356040
chr3_1 mRNA 31896495 31897251 31896495 ID=Msa0356040-mRNA-1;Parent=Msa0356040;Name=Msa0356040-mRNA-1;_AED=0.07;_eAED=0.07;_QI=0|0.5|0.33|1|1|1|3|337|59
chr3_1 exon 31896495 31896875 31896495 ID=Msa0356040-mRNA-1:exon:6426;Parent=Msa0356040-mRNA-1
chr3_1 exon 31897101 31897229 31897101 ID=Msa0356040-mRNA-1:exon:6425;Parent=Msa0356040-mRNA-1
chr3_1 exon 31897245 31897251 31897245 ID=Msa0356040-mRNA-1:exon:6424;Parent=Msa0356040-mRNA-1
chr3_1 CDS 31897245 31897251 31897245 ID=Msa0356040-mRNA-1:cds;Parent=Msa0356040-mRNA-1
chr3_1 CDS 31897101 31897229 31897101 ID=Msa0356040-mRNA-1:cds;Parent=Msa0356040-mRNA-1
chr3_1 CDS 31896832 31896875 31896832 ID=Msa0356040-mRNA-1:cds;Parent=Msa0356040-mRNA-1
chr3_1 three_prime_UTR 31896495 31896831 31896495 ID=Msa0356040-mRNA-1:three_prime_utr;Parent=Msa0356040-mRNA-1
Gene Sequence

>Msa0356040

ATGGAGGCTGAAGATACCAAGAGTTGTCCATGTGGTCACTGGCGACCCGCTGAAGATGAAAAACTTCCTCAACTTGTTGAACAATATGGTGCTCAAAATTGGAATTCAATTGCTGAAAAACTTCAAGGAAGACCAGGGAAACGTTGTAGGTTGAGGTGGTTCAATCAGCTAGATCCATGA

Protein sequence

>Msa0356040

MEAEDTKSCPCGHWRPAEDEKLPQLVEQYGAQNWNSIAEKLQGRPGKRCRLRWFNQLDP*