AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0356050


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0356050 MtrunA17_Chr3g0125801 91.228 57 5 0 3 59 8 64 9.50e-33 113
Msa0356050 MtrunA17_Chr7g0254261 68.519 54 17 0 6 59 84 137 6.16e-23 88.6
Msa0356050 MtrunA17_Chr4g0042501 72.222 54 15 0 6 59 100 153 1.67e-22 87.8
Msa0356050 MtrunA17_Chr5g0417621 70.370 54 16 0 6 59 129 182 3.63e-22 87.0
Msa0356050 MtrunA17_Chr6g0480581 72.549 51 14 0 9 59 2 52 3.81e-22 84.7
Msa0356050 MtrunA17_Chr6g0460671 60.000 55 22 0 5 59 93 147 7.08e-20 80.1
Msa0356050 MtrunA17_Chr6g0460661 64.583 48 17 0 12 59 17 64 1.06e-19 76.6
Msa0356050 MtrunA17_Chr4g0008551 58.333 48 20 0 12 59 39 86 6.64e-14 63.5
Msa0356050 MtrunA17_Chr5g0401731 54.167 48 22 0 12 59 16 63 2.51e-13 62.0
Msa0356050 MtrunA17_Chr4g0053431 54.167 48 22 0 12 59 6 53 3.46e-13 61.2
Msa0356050 MtrunA17_Chr6g0454921 54.167 48 22 0 12 59 16 63 4.18e-13 60.5
Msa0356050 MtrunA17_Chr3g0104501 53.846 52 24 0 8 59 130 181 5.32e-13 60.1
Msa0356050 MtrunA17_Chr5g0429661 56.250 48 21 0 12 59 123 170 9.59e-13 60.5
Msa0356050 MtrunA17_Chr5g0437321 52.083 48 23 0 12 59 20 67 2.12e-12 59.3
Msa0356050 MtrunA17_Chr3g0133601 54.167 48 22 0 12 59 38 85 5.11e-12 57.8
Msa0356050 MtrunA17_Chr4g0058991 54.167 48 22 0 12 59 210 257 3.54e-11 56.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0356050 AT1G73410.2 76.471 51 12 0 9 59 4 54 6.99e-24 88.6
Msa0356050 AT1G73410.1 76.471 51 12 0 9 59 4 54 1.65e-23 88.6
Msa0356050 AT1G17950.1 74.510 51 13 0 9 59 3 53 2.99e-23 88.2
Msa0356050 AT1G69560.1 75.000 52 13 0 8 59 104 155 5.08e-22 86.3
Msa0356050 AT1G69560.2 75.000 52 13 0 8 59 120 171 6.11e-22 86.3
Msa0356050 AT1G26780.1 79.167 48 10 0 12 59 99 146 8.01e-22 84.7
Msa0356050 AT1G26780.2 79.167 48 10 0 12 59 99 146 2.06e-21 84.7
Msa0356050 AT4G33450.1 69.231 52 16 0 8 59 16 67 2.48e-21 83.2
Msa0356050 AT5G17800.1 65.455 55 19 0 5 59 87 141 4.98e-21 83.6
Msa0356050 AT3G29020.1 62.963 54 20 0 6 59 60 113 7.82e-21 81.6
Msa0356050 AT3G29020.2 62.963 54 20 0 6 59 62 115 2.88e-20 81.3
Msa0356050 AT5G67300.1 54.167 48 22 0 12 59 7 54 8.35e-13 60.8
Msa0356050 AT5G39700.1 48.276 58 30 0 2 59 47 104 1.03e-12 59.3
Msa0356050 AT3G50060.1 52.083 48 23 0 12 59 7 54 3.51e-12 58.9
Msa0356050 AT5G40360.1 52.083 48 23 0 12 59 159 206 7.45e-12 58.2
Msa0356050 AT3G27785.1 52.083 48 23 0 12 59 190 237 1.07e-11 57.8
Msa0356050 AT2G39880.1 52.830 53 25 0 7 59 46 98 1.71e-11 57.4
Msa0356050 AT5G58850.1 48.148 54 28 0 6 59 100 153 2.48e-11 57.0
Msa0356050 AT3G09230.1 52.083 48 23 0 12 59 56 103 2.64e-11 56.6
Msa0356050 AT5G11050.1 49.057 53 27 0 7 59 101 153 2.68e-11 56.6
Msa0356050 AT2G23290.1 50.000 48 24 0 12 59 14 61 3.56e-11 56.2
Msa0356050 AT4G37260.1 50.000 48 24 0 12 59 14 61 4.95e-11 55.8
Msa0356050 AT3G09370.3 52.083 48 23 0 12 59 144 191 5.91e-11 55.8
Msa0356050 AT3G09370.1 52.083 48 23 0 12 59 131 178 6.33e-11 55.8
Msa0356050 AT3G09370.2 52.083 48 23 0 12 59 136 183 6.33e-11 55.8
Msa0356050 AT5G11510.2 50.000 48 24 0 12 59 82 129 8.91e-11 55.5
Msa0356050 AT5G11510.3 50.000 48 24 0 12 59 82 129 9.02e-11 55.5
Msa0356050 AT5G11510.1 50.000 48 24 0 12 59 82 129 9.02e-11 55.5
Msa0356050 AT5G11510.4 50.000 48 24 0 12 59 82 129 9.02e-11 55.5
Msa0356050 AT5G11510.5 50.000 48 24 0 12 59 82 129 9.17e-11 55.5

Find 34 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTCATTGAAATTTCTTATT+TGG 0.138946 3_1:-31928079 None:intergenic
ACAACATTATCAGTTCTTTC+AGG 0.182418 3_1:-31927850 None:intergenic
AACAATATGGTGCTCAAAAT+TGG 0.232647 3_1:+31927428 Msa0356050:CDS
TTCCCTTACACGACGAGAAT+TGG 0.299194 3_1:+31927977 Msa0356050:three_prime_UTR
CCTCAACTTGTTGAACAATA+TGG 0.300197 3_1:+31927415 Msa0356050:CDS
AAAGGTCTCTTGTTAATTCA+TGG 0.340427 3_1:-31927748 None:intergenic
CAAGAGGCAAGAGAACTTAA+AGG 0.364207 3_1:-31928031 None:intergenic
ACTTCTCTTGCCACATAACT+TGG 0.368979 3_1:-31927920 None:intergenic
CTTTAAGTTCTCTTGCCTCT+TGG 0.373629 3_1:+31928032 Msa0356050:three_prime_UTR
ATCGAATTCACATAAACAAA+TGG 0.389392 3_1:+31927806 Msa0356050:three_prime_UTR
GAATCATAGGCATGTTATAA+TGG 0.391053 3_1:+31927876 Msa0356050:three_prime_UTR
TATCCAATTCTCGTCGTGTA+AGG 0.396192 3_1:-31927980 None:intergenic
TCTTCTTCCTCTTCTGTGAA+AGG 0.428456 3_1:-31927766 None:intergenic
CAACATTATCAGTTCTTTCA+GGG 0.435680 3_1:-31927849 None:intergenic
ATAATGTTGTCAAGAATCAT+AGG 0.447489 3_1:+31927863 Msa0356050:three_prime_UTR
TAGGGAAACGTTGTAGGTTG+AGG 0.463106 3_1:+31927707 Msa0356050:intron
AACTTGTAGGGAAACGTTGT+AGG 0.477303 3_1:+31927701 Msa0356050:intron
TCGAATTCACATAAACAAAT+GGG 0.494256 3_1:+31927807 Msa0356050:three_prime_UTR
CAAGAGACCTTTCACAGAAG+AGG 0.503737 3_1:+31927759 Msa0356050:three_prime_UTR
AGAGTTGTCCATGTGGTCAC+TGG 0.512701 3_1:+31927368 Msa0356050:CDS
ACGAGAATTGGATATGAGAA+TGG 0.523637 3_1:+31927989 Msa0356050:three_prime_UTR
TATAATGGCTACAAAACAAA+GGG 0.531749 3_1:+31927891 Msa0356050:three_prime_UTR
GTGACCACATGGACAACTCT+TGG 0.543251 3_1:-31927365 None:intergenic
GTGTAAGGGAAATTAAGTAT+AGG 0.547906 3_1:-31927965 None:intergenic
ATCCAATTCTCGTCGTGTAA+GGG 0.553745 3_1:-31927979 None:intergenic
CCATATTGTTCAACAAGTTG+AGG 0.564383 3_1:-31927415 None:intergenic
AGGGAACAATCCAAGTTATG+TGG 0.586173 3_1:+31927910 Msa0356050:three_prime_UTR
TTATAATGGCTACAAAACAA+AGG 0.590289 3_1:+31927890 Msa0356050:three_prime_UTR
GGAAACGTTGTAGGTTGAGG+TGG 0.592608 3_1:+31927710 Msa0356050:CDS
GCGGGTCGCCAGTGACCACA+TGG 0.607822 3_1:-31927376 None:intergenic
ACTTCCATAATCCATATGGA+GGG 0.628080 3_1:+31927319 None:intergenic
CACAAAAGTAGAATTCCAAG+AGG 0.669761 3_1:-31928047 None:intergenic
GGAAGAAGAGAGACTACTTG+CGG 0.685866 3_1:+31927780 Msa0356050:three_prime_UTR
GATACCAAGAGTTGTCCATG+TGG 0.733953 3_1:+31927361 Msa0356050:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGAATTTGAAAATTAAATA+AGG - chr3_1:31927542-31927561 None:intergenic 10.0%
!! ATGAATTTGAAAATTAAATA+AGG - chr3_1:31927542-31927561 None:intergenic 10.0%
!! AATTTGAAAATTAAATAAGG+AGG - chr3_1:31927539-31927558 None:intergenic 15.0%
!! AATTTGAAAATTAAATAAGG+AGG - chr3_1:31927539-31927558 None:intergenic 15.0%
!! AATATTACATAATACATACC+TGG - chr3_1:31927484-31927503 None:intergenic 20.0%
!!! ATTTTTTCCTTTTAACTTGT+AGG + chr3_1:31927688-31927707 Msa0356050:intron 20.0%
!!! TTTTTTCCTTTTAACTTGTA+GGG + chr3_1:31927689-31927708 Msa0356050:intron 20.0%
!! AATATTACATAATACATACC+TGG - chr3_1:31927484-31927503 None:intergenic 20.0%
!!! ATTTTTTCCTTTTAACTTGT+AGG + chr3_1:31927688-31927707 Msa0356050:intron 20.0%
!!! TTTTTTCCTTTTAACTTGTA+GGG + chr3_1:31927689-31927708 Msa0356050:intron 20.0%
! ATCGAATTCACATAAACAAA+TGG + chr3_1:31927806-31927825 Msa0356050:three_prime_UTR 25.0%
! TCGAATTCACATAAACAAAT+GGG + chr3_1:31927807-31927826 Msa0356050:three_prime_UTR 25.0%
! ATAATGTTGTCAAGAATCAT+AGG + chr3_1:31927863-31927882 Msa0356050:three_prime_UTR 25.0%
! TTATAATGGCTACAAAACAA+AGG + chr3_1:31927890-31927909 Msa0356050:three_prime_UTR 25.0%
! TATAATGGCTACAAAACAAA+GGG + chr3_1:31927891-31927910 Msa0356050:three_prime_UTR 25.0%
! ATCGAATTCACATAAACAAA+TGG + chr3_1:31927806-31927825 Msa0356050:three_prime_UTR 25.0%
! TCGAATTCACATAAACAAAT+GGG + chr3_1:31927807-31927826 Msa0356050:three_prime_UTR 25.0%
! ATAATGTTGTCAAGAATCAT+AGG + chr3_1:31927863-31927882 Msa0356050:three_prime_UTR 25.0%
! TTATAATGGCTACAAAACAA+AGG + chr3_1:31927890-31927909 Msa0356050:three_prime_UTR 25.0%
! TATAATGGCTACAAAACAAA+GGG + chr3_1:31927891-31927910 Msa0356050:three_prime_UTR 25.0%
AACAATATGGTGCTCAAAAT+TGG + chr3_1:31927428-31927447 Msa0356050:CDS 30.0%
AAAGGTCTCTTGTTAATTCA+TGG - chr3_1:31927751-31927770 None:intergenic 30.0%
CAACATTATCAGTTCTTTCA+GGG - chr3_1:31927852-31927871 None:intergenic 30.0%
ACAACATTATCAGTTCTTTC+AGG - chr3_1:31927853-31927872 None:intergenic 30.0%
GAATCATAGGCATGTTATAA+TGG + chr3_1:31927876-31927895 Msa0356050:three_prime_UTR 30.0%
GTGTAAGGGAAATTAAGTAT+AGG - chr3_1:31927968-31927987 None:intergenic 30.0%
AACAATATGGTGCTCAAAAT+TGG + chr3_1:31927428-31927447 Msa0356050:CDS 30.0%
AAAGGTCTCTTGTTAATTCA+TGG - chr3_1:31927751-31927770 None:intergenic 30.0%
CAACATTATCAGTTCTTTCA+GGG - chr3_1:31927852-31927871 None:intergenic 30.0%
ACAACATTATCAGTTCTTTC+AGG - chr3_1:31927853-31927872 None:intergenic 30.0%
GAATCATAGGCATGTTATAA+TGG + chr3_1:31927876-31927895 Msa0356050:three_prime_UTR 30.0%
GTGTAAGGGAAATTAAGTAT+AGG - chr3_1:31927968-31927987 None:intergenic 30.0%
! AGGAAGTTTTTCATCTTCAG+CGG - chr3_1:31927398-31927417 None:intergenic 35.0%
CCATATTGTTCAACAAGTTG+AGG - chr3_1:31927418-31927437 None:intergenic 35.0%
!! CCTCAACTTGTTGAACAATA+TGG + chr3_1:31927415-31927434 Msa0356050:CDS 35.0%
TCAACTGCTGAAAAACTTCA+AGG + chr3_1:31927454-31927473 Msa0356050:CDS 35.0%
ACGTTTCCCTACAAGTTAAA+AGG - chr3_1:31927698-31927717 None:intergenic 35.0%
ACGAGAATTGGATATGAGAA+TGG + chr3_1:31927989-31928008 Msa0356050:three_prime_UTR 35.0%
CACAAAAGTAGAATTCCAAG+AGG - chr3_1:31928050-31928069 None:intergenic 35.0%
! AGGAAGTTTTTCATCTTCAG+CGG - chr3_1:31927398-31927417 None:intergenic 35.0%
CCATATTGTTCAACAAGTTG+AGG - chr3_1:31927418-31927437 None:intergenic 35.0%
!! CCTCAACTTGTTGAACAATA+TGG + chr3_1:31927415-31927434 Msa0356050:CDS 35.0%
TCAACTGCTGAAAAACTTCA+AGG + chr3_1:31927454-31927473 Msa0356050:CDS 35.0%
ACGTTTCCCTACAAGTTAAA+AGG - chr3_1:31927698-31927717 None:intergenic 35.0%
ACGAGAATTGGATATGAGAA+TGG + chr3_1:31927989-31928008 Msa0356050:three_prime_UTR 35.0%
CACAAAAGTAGAATTCCAAG+AGG - chr3_1:31928050-31928069 None:intergenic 35.0%
! GGAAGTTTTTCATCTTCAGC+GGG - chr3_1:31927397-31927416 None:intergenic 40.0%
GAAAAACTTCAAGGAAGACC+AGG + chr3_1:31927463-31927482 Msa0356050:CDS 40.0%
AACTTGTAGGGAAACGTTGT+AGG + chr3_1:31927701-31927720 Msa0356050:intron 40.0%
TCTTCTTCCTCTTCTGTGAA+AGG - chr3_1:31927769-31927788 None:intergenic 40.0%
AGGGAACAATCCAAGTTATG+TGG + chr3_1:31927910-31927929 Msa0356050:three_prime_UTR 40.0%
ACTTCTCTTGCCACATAACT+TGG - chr3_1:31927923-31927942 None:intergenic 40.0%
ATCCAATTCTCGTCGTGTAA+GGG - chr3_1:31927982-31928001 None:intergenic 40.0%
TATCCAATTCTCGTCGTGTA+AGG - chr3_1:31927983-31928002 None:intergenic 40.0%
CAAGAGGCAAGAGAACTTAA+AGG - chr3_1:31928034-31928053 None:intergenic 40.0%
! CTTTAAGTTCTCTTGCCTCT+TGG + chr3_1:31928032-31928051 Msa0356050:three_prime_UTR 40.0%
! GGAAGTTTTTCATCTTCAGC+GGG - chr3_1:31927397-31927416 None:intergenic 40.0%
GAAAAACTTCAAGGAAGACC+AGG + chr3_1:31927463-31927482 Msa0356050:CDS 40.0%
AACTTGTAGGGAAACGTTGT+AGG + chr3_1:31927701-31927720 Msa0356050:intron 40.0%
TCTTCTTCCTCTTCTGTGAA+AGG - chr3_1:31927769-31927788 None:intergenic 40.0%
AGGGAACAATCCAAGTTATG+TGG + chr3_1:31927910-31927929 Msa0356050:three_prime_UTR 40.0%
ACTTCTCTTGCCACATAACT+TGG - chr3_1:31927923-31927942 None:intergenic 40.0%
ATCCAATTCTCGTCGTGTAA+GGG - chr3_1:31927982-31928001 None:intergenic 40.0%
TATCCAATTCTCGTCGTGTA+AGG - chr3_1:31927983-31928002 None:intergenic 40.0%
CAAGAGGCAAGAGAACTTAA+AGG - chr3_1:31928034-31928053 None:intergenic 40.0%
! CTTTAAGTTCTCTTGCCTCT+TGG + chr3_1:31928032-31928051 Msa0356050:three_prime_UTR 40.0%
GATACCAAGAGTTGTCCATG+TGG + chr3_1:31927361-31927380 Msa0356050:CDS 45.0%
TAGGGAAACGTTGTAGGTTG+AGG + chr3_1:31927707-31927726 Msa0356050:intron 45.0%
CAAGAGACCTTTCACAGAAG+AGG + chr3_1:31927759-31927778 Msa0356050:three_prime_UTR 45.0%
GGAAGAAGAGAGACTACTTG+CGG + chr3_1:31927780-31927799 Msa0356050:three_prime_UTR 45.0%
TTCCCTTACACGACGAGAAT+TGG + chr3_1:31927977-31927996 Msa0356050:three_prime_UTR 45.0%
GATACCAAGAGTTGTCCATG+TGG + chr3_1:31927361-31927380 Msa0356050:CDS 45.0%
TAGGGAAACGTTGTAGGTTG+AGG + chr3_1:31927707-31927726 Msa0356050:intron 45.0%
CAAGAGACCTTTCACAGAAG+AGG + chr3_1:31927759-31927778 Msa0356050:three_prime_UTR 45.0%
GGAAGAAGAGAGACTACTTG+CGG + chr3_1:31927780-31927799 Msa0356050:three_prime_UTR 45.0%
TTCCCTTACACGACGAGAAT+TGG + chr3_1:31927977-31927996 Msa0356050:three_prime_UTR 45.0%
!! TATTATGTAATATTAATTAA+AGG + chr3_1:31927490-31927509 Msa0356050:intron 5.0%
!! TATTATGTAATATTAATTAA+AGG + chr3_1:31927490-31927509 Msa0356050:intron 5.0%
GTGACCACATGGACAACTCT+TGG - chr3_1:31927368-31927387 None:intergenic 50.0%
AGAGTTGTCCATGTGGTCAC+TGG + chr3_1:31927368-31927387 Msa0356050:CDS 50.0%
! GGAAACGTTGTAGGTTGAGG+TGG + chr3_1:31927710-31927729 Msa0356050:CDS 50.0%
GTGACCACATGGACAACTCT+TGG - chr3_1:31927368-31927387 None:intergenic 50.0%
AGAGTTGTCCATGTGGTCAC+TGG + chr3_1:31927368-31927387 Msa0356050:CDS 50.0%
! GGAAACGTTGTAGGTTGAGG+TGG + chr3_1:31927710-31927729 Msa0356050:CDS 50.0%
GCGGGTCGCCAGTGACCACA+TGG - chr3_1:31927379-31927398 None:intergenic 70.0%
GCGGGTCGCCAGTGACCACA+TGG - chr3_1:31927379-31927398 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 31927334 31928090 31927334 ID=Msa0356050;Name=Msa0356050
chr3_1 mRNA 31927334 31928090 31927334 ID=Msa0356050-mRNA-1;Parent=Msa0356050;Name=Msa0356050-mRNA-1;_AED=0.07;_eAED=0.07;_QI=0|0.5|0.33|1|1|1|3|337|59
chr3_1 exon 31927334 31927340 31927334 ID=Msa0356050-mRNA-1:exon:6421;Parent=Msa0356050-mRNA-1
chr3_1 exon 31927356 31927484 31927356 ID=Msa0356050-mRNA-1:exon:6422;Parent=Msa0356050-mRNA-1
chr3_1 exon 31927710 31928090 31927710 ID=Msa0356050-mRNA-1:exon:6423;Parent=Msa0356050-mRNA-1
chr3_1 CDS 31927334 31927340 31927334 ID=Msa0356050-mRNA-1:cds;Parent=Msa0356050-mRNA-1
chr3_1 CDS 31927356 31927484 31927356 ID=Msa0356050-mRNA-1:cds;Parent=Msa0356050-mRNA-1
chr3_1 CDS 31927710 31927753 31927710 ID=Msa0356050-mRNA-1:cds;Parent=Msa0356050-mRNA-1
chr3_1 three_prime_UTR 31927754 31928090 31927754 ID=Msa0356050-mRNA-1:three_prime_utr;Parent=Msa0356050-mRNA-1
Gene Sequence

>Msa0356050

ATGGAGGCTGAAGATACCAAGAGTTGTCCATGTGGTCACTGGCGACCCGCTGAAGATGAAAAACTTCCTCAACTTGTTGAACAATATGGTGCTCAAAATTGGAATTCAACTGCTGAAAAACTTCAAGGAAGACCAGGGAAACGTTGTAGGTTGAGGTGGTTCAATCAGCTAGATCCATGA

Protein sequence

>Msa0356050

MEAEDTKSCPCGHWRPAEDEKLPQLVEQYGAQNWNSTAEKLQGRPGKRCRLRWFNQLDP*