AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0356740


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0356740 MtrunA17_Chr3g0093571 100.000 104 0 0 1 104 1 104 5.77e-68 211
Msa0356740 MtrunA17_Chr3g0093391 95.098 102 5 0 3 104 1 102 9.19e-63 198
Msa0356740 MtrunA17_Chr4g0039141 78.125 64 14 0 1 64 79 142 2.06e-30 106
Msa0356740 MtrunA17_Chr4g0051261 56.522 69 30 0 25 93 4 72 8.88e-22 86.7
Msa0356740 MtrunA17_Chr2g0293491 56.522 69 30 0 25 93 4 72 1.42e-21 86.3
Msa0356740 MtrunA17_Chr4g0057791 60.000 65 26 0 20 84 213 277 5.12e-20 83.2
Msa0356740 MtrunA17_Chr8g0377071 56.000 75 33 0 17 91 377 451 1.58e-19 82.0
Msa0356740 MtrunA17_Chr3g0122781 58.108 74 31 0 18 91 409 482 2.34e-19 81.6
Msa0356740 MtrunA17_Chr4g0061141 58.333 72 28 2 23 93 103 173 2.90e-18 78.6
Msa0356740 MtrunA17_Chr1g0191221 56.000 75 31 2 20 93 117 190 4.34e-18 77.8
Msa0356740 MtrunA17_Chr2g0286421 56.000 75 31 2 20 93 97 170 2.78e-17 75.5
Msa0356740 MtrunA17_Chr2g0317501 57.333 75 30 2 20 93 129 202 3.44e-17 75.5
Msa0356740 MtrunA17_Chr7g0273601 54.667 75 32 2 20 93 102 175 4.01e-17 75.1
Msa0356740 MtrunA17_Chr7g0218071 54.167 72 31 2 23 93 118 188 7.39e-17 74.3
Msa0356740 MtrunA17_Chr8g0385971 54.667 75 32 2 20 93 85 158 8.06e-17 74.3
Msa0356740 MtrunA17_Chr4g0066691 56.000 75 31 2 20 93 99 172 9.06e-17 73.9
Msa0356740 MtrunA17_Chr4g0002001 45.122 82 44 1 25 105 4 85 9.24e-17 73.6
Msa0356740 MtrunA17_Chr3g0131861 54.667 75 32 2 20 93 92 165 1.02e-16 73.9
Msa0356740 MtrunA17_Chr1g0187371 52.174 69 32 1 23 90 16 84 1.20e-16 73.2
Msa0356740 MtrunA17_Chr6g0453711 50.000 72 34 2 23 93 108 178 6.09e-16 71.6
Msa0356740 MtrunA17_Chr4g0040141 49.333 75 36 2 20 93 124 197 1.81e-15 70.5
Msa0356740 MtrunA17_Chr1g0194881 88.889 36 4 0 1 36 1 36 1.90e-15 69.7
Msa0356740 MtrunA17_Chr6g0483151 50.000 68 34 0 20 87 9 76 2.70e-15 69.7
Msa0356740 MtrunA17_Chr5g0396361 50.667 75 35 2 20 93 135 208 3.07e-15 69.7
Msa0356740 MtrunA17_Chr3g0099951 47.143 70 35 1 23 90 12 81 2.42e-14 67.0
Msa0356740 MtrunA17_Chr2g0324461 46.667 75 38 2 20 93 136 209 1.20e-13 65.1
Msa0356740 MtrunA17_Chr1g0201571 41.333 75 44 0 19 93 120 194 5.66e-13 63.2
Msa0356740 MtrunA17_Chr3g0130781 42.029 69 37 1 25 90 18 86 1.16e-11 59.7
Msa0356740 MtrunA17_Chr8g0383451 48.438 64 31 2 31 93 4 66 2.89e-11 58.5
Msa0356740 MtrunA17_Chr8g0339221 43.939 66 32 2 28 91 7 69 4.49e-11 57.8
Msa0356740 MtrunA17_Chr3g0141871 45.312 64 35 0 28 91 35 98 9.84e-11 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0356740 AT5G10270.1 83.810 105 16 1 1 104 1 105 5.94e-53 172
Msa0356740 AT5G64960.1 82.857 105 17 1 1 104 1 105 1.76e-51 169
Msa0356740 AT1G67580.1 58.667 75 31 0 17 91 398 472 1.04e-25 100
Msa0356740 AT1G67580.2 58.667 75 31 0 17 91 398 472 1.04e-25 100
Msa0356740 AT3G48750.1 56.944 72 31 0 22 93 1 72 1.99e-22 88.6
Msa0356740 AT5G63370.9 49.451 91 38 2 6 92 148 234 3.47e-21 86.7
Msa0356740 AT5G63370.5 50.000 90 37 2 6 91 282 367 3.76e-21 87.0
Msa0356740 AT5G63370.10 50.000 90 37 2 6 91 282 367 3.76e-21 87.0
Msa0356740 AT5G63370.7 50.000 90 37 2 6 91 282 367 4.13e-21 87.0
Msa0356740 AT5G63370.8 50.000 90 37 2 6 91 282 367 4.13e-21 87.0
Msa0356740 AT5G63370.4 50.000 90 37 2 6 91 282 367 4.13e-21 87.0
Msa0356740 AT5G63370.6 50.000 90 37 2 6 91 282 367 4.13e-21 87.0
Msa0356740 AT5G63370.1 50.000 90 37 2 6 91 282 367 4.13e-21 87.0
Msa0356740 AT5G63370.2 49.451 91 38 2 6 92 148 234 4.30e-21 86.7
Msa0356740 AT5G63370.3 49.451 91 38 2 6 92 148 234 4.30e-21 86.7
Msa0356740 AT1G18670.2 63.889 72 24 2 23 93 129 199 1.51e-19 82.4
Msa0356740 AT1G18670.3 63.889 72 24 2 23 93 129 199 2.11e-19 82.0
Msa0356740 AT1G18670.1 63.889 72 24 2 23 93 129 199 2.15e-19 82.0
Msa0356740 AT1G33770.1 56.000 75 31 2 20 93 136 209 9.40e-19 80.1
Msa0356740 AT3G05050.3 57.333 75 30 2 20 93 133 206 2.45e-18 79.0
Msa0356740 AT3G05050.2 57.333 75 30 2 20 93 133 206 2.45e-18 79.0
Msa0356740 AT3G05050.1 57.333 75 30 2 20 93 133 206 2.45e-18 79.0
Msa0356740 AT1G76540.1 53.521 71 32 1 21 90 10 80 8.61e-18 76.6
Msa0356740 AT1G71530.1 54.545 77 29 2 20 93 142 215 1.07e-17 77.0
Msa0356740 AT1G57700.1 54.667 75 32 2 20 93 141 214 1.30e-17 77.0
Msa0356740 AT5G39420.1 53.521 71 33 0 23 93 103 173 2.25e-17 76.3
Msa0356740 AT4G10010.2 53.247 77 30 2 20 93 151 224 2.38e-17 76.3
Msa0356740 AT1G74330.3 58.333 72 28 2 23 93 119 189 2.63e-17 76.3
Msa0356740 AT1G74330.1 58.333 72 28 2 23 93 119 189 2.64e-17 76.3
Msa0356740 AT1G74330.2 58.333 72 28 2 23 93 119 189 3.30e-17 75.9
Msa0356740 AT1G66750.1 47.297 74 39 0 14 87 2 75 3.31e-17 75.5
Msa0356740 AT1G71530.4 54.545 77 29 2 20 93 142 215 3.40e-17 75.5
Msa0356740 AT5G64960.2 87.500 40 5 0 65 104 13 52 4.04e-17 75.5
Msa0356740 AT1G71530.3 54.545 77 29 2 20 93 142 215 4.27e-17 75.5
Msa0356740 AT1G71530.2 54.545 77 29 2 20 93 142 215 6.36e-17 74.7
Msa0356740 AT5G50860.2 53.333 75 33 2 20 93 109 182 1.12e-16 74.3
Msa0356740 AT1G53050.2 54.667 75 32 2 20 93 129 202 1.52e-16 73.9
Msa0356740 AT1G53050.1 54.667 75 32 2 20 93 129 202 1.52e-16 73.9
Msa0356740 AT5G50860.1 53.333 75 33 2 20 93 109 182 2.21e-16 73.6
Msa0356740 AT1G54610.1 53.333 75 33 2 20 93 113 186 2.27e-16 73.6
Msa0356740 AT1G54610.3 53.333 75 33 2 20 93 113 186 2.27e-16 73.6
Msa0356740 AT1G54610.2 53.333 75 33 2 20 93 113 186 2.27e-16 73.6
Msa0356740 AT1G09600.1 51.351 74 36 0 20 93 158 231 2.58e-16 73.2
Msa0356740 AT1G20930.1 50.704 71 34 1 21 90 12 82 3.28e-16 72.4
Msa0356740 AT1G73690.1 48.529 68 35 0 20 87 6 73 8.57e-16 71.6
Msa0356740 AT1G18040.1 46.575 73 39 0 15 87 2 74 1.32e-15 70.9
Msa0356740 AT1G03740.2 48.684 76 37 2 20 94 208 282 2.29e-15 70.5
Msa0356740 AT1G03740.1 48.684 76 37 2 20 94 208 282 2.51e-15 70.5
Msa0356740 AT2G38620.2 44.706 85 37 1 25 99 4 88 4.57e-15 69.3
Msa0356740 AT2G38620.3 44.706 85 37 1 25 99 4 88 5.13e-15 68.6
Msa0356740 AT2G38620.1 44.706 85 37 1 25 99 4 88 5.13e-15 68.6
Msa0356740 AT3G54180.1 58.182 55 23 0 25 79 4 58 3.94e-14 66.6
Msa0356740 AT3G01085.3 51.429 70 32 2 25 93 115 183 8.15e-14 66.2
Msa0356740 AT3G01085.1 51.429 70 32 2 25 93 115 183 8.39e-14 66.2
Msa0356740 AT3G01085.2 51.429 70 32 2 25 93 115 183 8.39e-14 66.2
Msa0356740 AT5G44290.6 45.333 75 39 2 20 93 132 205 9.43e-14 65.9
Msa0356740 AT5G44290.7 45.333 75 39 2 20 93 132 205 1.32e-13 65.5
Msa0356740 AT5G44290.2 45.333 75 39 2 20 93 132 205 1.89e-13 65.1
Msa0356740 AT5G44290.5 45.333 75 39 2 20 93 132 205 1.89e-13 65.1
Msa0356740 AT5G44290.4 45.333 75 39 2 20 93 132 205 1.89e-13 65.1
Msa0356740 AT5G44290.1 45.333 75 39 2 20 93 132 205 1.89e-13 65.1
Msa0356740 AT5G44290.3 45.333 75 39 2 20 93 132 205 1.89e-13 65.1
Msa0356740 AT4G22940.1 48.101 79 35 3 14 91 96 169 8.82e-12 60.5
Msa0356740 AT4G22940.2 48.193 83 37 3 7 88 89 166 9.86e-12 60.1

Find 34 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAATCGGCGAAGGCACTTA+TGG 0.185430 3_1:+36773621 Msa0356740:CDS
TACTTGCAGTATGGTTTATA+TGG 0.207853 3_1:+36773718 Msa0356740:intron
TGAAAATGTAATCAAGTTAA+AGG 0.254582 3_1:+36773956 Msa0356740:CDS
GGAATCCATGGGATATGTTT+AGG 0.279098 3_1:+36773368 Msa0356740:five_prime_UTR
ATTTCACGTATAGCAGTAAT+AGG 0.291287 3_1:-36773909 None:intergenic
ATCGATCAATCATCAATTCT+AGG 0.324139 3_1:+36773488 Msa0356740:five_prime_UTR
GAATCCATGGGATATGTTTA+GGG 0.404527 3_1:+36773369 Msa0356740:five_prime_UTR
GGGTTTATTGAGTGAGTTTG+TGG 0.417115 3_1:+36773509 Msa0356740:five_prime_UTR
ATGAATATATGTGGAATCCA+TGG 0.429338 3_1:+36773356 Msa0356740:five_prime_UTR
TATAGGTAAGATTGAATTGA+AGG 0.436009 3_1:+36773421 Msa0356740:five_prime_UTR
TTTGAGAAACTCGAACAAAT+CGG 0.450626 3_1:+36773606 Msa0356740:CDS
AAATCGGCGAAGGCACTTAT+GGG 0.452605 3_1:+36773622 Msa0356740:CDS
GTAATGACAATGGCGGGTCC+AGG 0.454950 3_1:+36773531 Msa0356740:exon
AAGGAAATCGTGACATCGCC+AGG 0.468018 3_1:+36773975 Msa0356740:CDS
ATGGCTCGAGAGATCGAAAC+TGG 0.468440 3_1:+36773737 Msa0356740:CDS
TCGATCACGTTCAATTGCCC+TGG 0.481036 3_1:-36773549 None:intergenic
TCGATCAATCATCAATTCTA+GGG 0.502054 3_1:+36773489 Msa0356740:five_prime_UTR
GTATGGATAATGAAAGAGAA+GGG 0.512036 3_1:+36773786 Msa0356740:CDS
AAAGATGATCAAGGAAGGCC+AGG 0.518923 3_1:+36774005 Msa0356740:CDS
TGCTCTCAAGAAAATACGTA+TGG 0.537503 3_1:+36773769 Msa0356740:CDS
GATGATTGATCGATCAATGA+TGG 0.556063 3_1:-36773479 None:intergenic
CGTATGGATAATGAAAGAGA+AGG 0.557605 3_1:+36773785 Msa0356740:CDS
ATTGATCGATCAATGATGGA+TGG 0.575260 3_1:-36773475 None:intergenic
TAATGACAATGGCGGGTCCA+GGG 0.590746 3_1:+36773532 Msa0356740:exon
TGAATATATGTGGAATCCAT+GGG 0.599937 3_1:+36773357 Msa0356740:five_prime_UTR
TTGATCGATCAATGATGGAT+GGG 0.610615 3_1:-36773474 None:intergenic
TCTTCCCTAAACATATCCCA+TGG 0.615597 3_1:-36773373 None:intergenic
TTTGTGGTAATGACAATGGC+GGG 0.638401 3_1:+36773525 Msa0356740:exon
TATGGATAATGAAAGAGAAG+GGG 0.649789 3_1:+36773787 Msa0356740:CDS
AAACTCGAACAAATCGGCGA+AGG 0.657326 3_1:+36773612 Msa0356740:CDS
ACGCTACGAGATCCACGCGA+AGG 0.686881 3_1:-36773576 None:intergenic
GTGATCGAATCACCTTCGCG+TGG 0.695133 3_1:+36773564 Msa0356740:CDS
TGAGTTTGTGGTAATGACAA+TGG 0.701884 3_1:+36773521 Msa0356740:exon
GTTTGTGGTAATGACAATGG+CGG 0.759832 3_1:+36773524 Msa0356740:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATAAATGAATATATG+TGG + chr3_1:36773347-36773366 Msa0356740:five_prime_UTR 10.0%
!!! TACTATATTTTTTATTTTTG+AGG - chr3_1:36773403-36773422 None:intergenic 10.0%
!! TGAAAATGTAATCAAGTTAA+AGG + chr3_1:36773956-36773975 Msa0356740:CDS 20.0%
! TATAGGTAAGATTGAATTGA+AGG + chr3_1:36773421-36773440 Msa0356740:five_prime_UTR 25.0%
!!! GTTTTCTCTTTGATTTTGTT+GGG - chr3_1:36773455-36773474 None:intergenic 25.0%
! AGAAGAATTGAATGAAGAAA+AGG - chr3_1:36773663-36773682 None:intergenic 25.0%
!!! ATGTTTTTATACTTGCAGTA+TGG + chr3_1:36773709-36773728 Msa0356740:intron 25.0%
! AATTACACATAATCACAGAT+AGG - chr3_1:36773849-36773868 None:intergenic 25.0%
ATGAATATATGTGGAATCCA+TGG + chr3_1:36773356-36773375 Msa0356740:five_prime_UTR 30.0%
TGAATATATGTGGAATCCAT+GGG + chr3_1:36773357-36773376 Msa0356740:five_prime_UTR 30.0%
!! GGTTTTCTCTTTGATTTTGT+TGG - chr3_1:36773456-36773475 None:intergenic 30.0%
ATCGATCAATCATCAATTCT+AGG + chr3_1:36773488-36773507 Msa0356740:five_prime_UTR 30.0%
TCGATCAATCATCAATTCTA+GGG + chr3_1:36773489-36773508 Msa0356740:five_prime_UTR 30.0%
TTTGAGAAACTCGAACAAAT+CGG + chr3_1:36773606-36773625 Msa0356740:CDS 30.0%
TACTTGCAGTATGGTTTATA+TGG + chr3_1:36773718-36773737 Msa0356740:intron 30.0%
GTATGGATAATGAAAGAGAA+GGG + chr3_1:36773786-36773805 Msa0356740:CDS 30.0%
TATGGATAATGAAAGAGAAG+GGG + chr3_1:36773787-36773806 Msa0356740:CDS 30.0%
ATTTCACGTATAGCAGTAAT+AGG - chr3_1:36773912-36773931 None:intergenic 30.0%
GAATCCATGGGATATGTTTA+GGG + chr3_1:36773369-36773388 Msa0356740:five_prime_UTR 35.0%
!! CTCTTTGATTTTGTTGGGTT+AGG - chr3_1:36773450-36773469 None:intergenic 35.0%
!! TTGATCGATCAATGATGGAT+GGG - chr3_1:36773477-36773496 None:intergenic 35.0%
! ATTGATCGATCAATGATGGA+TGG - chr3_1:36773478-36773497 None:intergenic 35.0%
GATGATTGATCGATCAATGA+TGG - chr3_1:36773482-36773501 None:intergenic 35.0%
! TGAGTTTGTGGTAATGACAA+TGG + chr3_1:36773521-36773540 Msa0356740:exon 35.0%
TGCTCTCAAGAAAATACGTA+TGG + chr3_1:36773769-36773788 Msa0356740:CDS 35.0%
CGTATGGATAATGAAAGAGA+AGG + chr3_1:36773785-36773804 Msa0356740:CDS 35.0%
!! TTCCTTGATCATCTTTTTCG+GGG - chr3_1:36774001-36774020 None:intergenic 35.0%
! CTTCCTTGATCATCTTTTTC+GGG - chr3_1:36774002-36774021 None:intergenic 35.0%
! CCTTCCTTGATCATCTTTTT+CGG - chr3_1:36774003-36774022 None:intergenic 35.0%
GGAATCCATGGGATATGTTT+AGG + chr3_1:36773368-36773387 Msa0356740:five_prime_UTR 40.0%
TCTTCCCTAAACATATCCCA+TGG - chr3_1:36773376-36773395 None:intergenic 40.0%
GGGTTTATTGAGTGAGTTTG+TGG + chr3_1:36773509-36773528 Msa0356740:five_prime_UTR 40.0%
! GTTTGTGGTAATGACAATGG+CGG + chr3_1:36773524-36773543 Msa0356740:exon 40.0%
! TTTGTGGTAATGACAATGGC+GGG + chr3_1:36773525-36773544 Msa0356740:exon 40.0%
CCGAAAAAGATGATCAAGGA+AGG + chr3_1:36774000-36774019 Msa0356740:CDS 40.0%
AAACTCGAACAAATCGGCGA+AGG + chr3_1:36773612-36773631 Msa0356740:CDS 45.0%
!! AAATCGGCGAAGGCACTTAT+GGG + chr3_1:36773622-36773641 Msa0356740:CDS 45.0%
AAAGATGATCAAGGAAGGCC+AGG + chr3_1:36774005-36774024 Msa0356740:CDS 45.0%
!! AAAAATAAAAAATATAGTAT+AGG + chr3_1:36773404-36773423 Msa0356740:five_prime_UTR 5.0%
!! TAATGACAATGGCGGGTCCA+GGG + chr3_1:36773532-36773551 Msa0356740:exon 50.0%
TCGATCACGTTCAATTGCCC+TGG - chr3_1:36773552-36773571 None:intergenic 50.0%
!! CAAATCGGCGAAGGCACTTA+TGG + chr3_1:36773621-36773640 Msa0356740:CDS 50.0%
ATGGCTCGAGAGATCGAAAC+TGG + chr3_1:36773737-36773756 Msa0356740:CDS 50.0%
AAGGAAATCGTGACATCGCC+AGG + chr3_1:36773975-36773994 Msa0356740:CDS 50.0%
!!! TGATCATCTTTTTCGGGGCC+TGG - chr3_1:36773996-36774015 None:intergenic 50.0%
GGCCCCGAAAAAGATGATCA+AGG + chr3_1:36773996-36774015 Msa0356740:CDS 50.0%
!! GTAATGACAATGGCGGGTCC+AGG + chr3_1:36773531-36773550 Msa0356740:exon 55.0%
GTGATCGAATCACCTTCGCG+TGG + chr3_1:36773564-36773583 Msa0356740:CDS 55.0%
ACGCTACGAGATCCACGCGA+AGG - chr3_1:36773579-36773598 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 36773290 36774028 36773290 ID=Msa0356740;Name=Msa0356740
chr3_1 mRNA 36773290 36774028 36773290 ID=Msa0356740-mRNA-1;Parent=Msa0356740;Name=Msa0356740-mRNA-1;_AED=0.08;_eAED=0.08;_QI=244|1|0.66|1|1|0.66|3|0|105
chr3_1 exon 36773290 36773643 36773290 ID=Msa0356740-mRNA-1:exon:6702;Parent=Msa0356740-mRNA-1
chr3_1 exon 36773727 36773808 36773727 ID=Msa0356740-mRNA-1:exon:6703;Parent=Msa0356740-mRNA-1
chr3_1 exon 36773906 36774028 36773906 ID=Msa0356740-mRNA-1:exon:6704;Parent=Msa0356740-mRNA-1
chr3_1 five_prime_UTR 36773290 36773533 36773290 ID=Msa0356740-mRNA-1:five_prime_utr;Parent=Msa0356740-mRNA-1
chr3_1 CDS 36773534 36773643 36773534 ID=Msa0356740-mRNA-1:cds;Parent=Msa0356740-mRNA-1
chr3_1 CDS 36773727 36773808 36773727 ID=Msa0356740-mRNA-1:cds;Parent=Msa0356740-mRNA-1
chr3_1 CDS 36773906 36774028 36773906 ID=Msa0356740-mRNA-1:cds;Parent=Msa0356740-mRNA-1
Gene Sequence

>Msa0356740

ATGACAATGGCGGGTCCAGGGCAATTGAACGTGATCGAATCACCTTCGCGTGGATCTCGTAGCGTCGATTGCTTTGAGAAACTCGAACAAATCGGCGAAGGCACTTATGGTATGGTTTATATGGCTCGAGAGATCGAAACTGGTGAAATTGTTGCTCTCAAGAAAATACGTATGGATAATGAAAGAGAAGGGTTTCCTATTACTGCTATACGTGAAATCAAAATTCTAAAGAAACTACATCATGAAAATGTAATCAAGTTAAAGGAAATCGTGACATCGCCAGGCCCCGAAAAAGATGATCAAGGAAGGCCAGGC

Protein sequence

>Msa0356740

MTMAGPGQLNVIESPSRGSRSVDCFEKLEQIGEGTYGMVYMAREIETGEIVALKKIRMDNEREGFPITAIREIKILKKLHHENVIKLKEIVTSPGPEKDDQGRPG