AlfalfaGEDB | Msa0362110

M. sativa cultivar zhongmu-4 / Msa0362110

KEGG: K08867
GO: GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0140096 GO:1901564
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0362110	AES70503.2	97.306	297	8	0	1	297	1	297	0.0	608

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0362110	sp\|Q6ICW6\|WNK11_ARATH	79.787	282	57	0	6	287	11	292	1.57e-176	493

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0362110	G7J1J0	97.306	297	8	0	1	297	1	297	0.0	608

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0362110	MtrunA17_Chr3g0101091	97.306	297	8	0	1	297	1	297	0.0	608
Msa0362110	MtrunA17_Chr3g0101081	97.306	297	8	0	1	297	1	297	0.0	608
Msa0362110	MtrunA17_Chr3g0101111	97.297	296	8	0	1	296	1	296	0.0	607
Msa0362110	MtrunA17_Chr7g0225201	89.527	296	31	0	1	296	2	297	0.0	563
Msa0362110	MtrunA17_Chr3g0132681	87.458	295	36	1	1	294	1	295	0.0	540
Msa0362110	MtrunA17_Chr5g0445271	83.451	284	47	0	1	284	2	285	0.0	513
Msa0362110	MtrunA17_Chr5g0447731	75.887	282	68	0	10	291	7	288	7.46e-170	472
Msa0362110	MtrunA17_Chr7g0273851	62.191	283	104	2	10	290	8	289	2.41e-125	374
Msa0362110	MtrunA17_Chr1g0205471	63.121	282	99	4	11	290	10	288	8.59e-123	367
Msa0362110	MtrunA17_Chr1g0190951	61.837	283	105	2	10	290	8	289	1.06e-122	367

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0362110	AT5G55560.1	79.787	282	57	0	6	287	11	292	1.60e-177	493
Msa0362110	AT3G48260.1	64.945	271	95	0	14	284	10	280	8.80e-129	376
Msa0362110	AT3G04910.1	62.544	283	103	2	10	290	8	289	1.22e-123	369
Msa0362110	AT3G22420.1	58.696	276	113	1	11	286	9	283	3.97e-120	356
Msa0362110	AT3G22420.5	58.696	276	113	1	11	286	9	283	8.46e-120	356
Msa0362110	AT5G28080.2	59.273	275	111	1	10	284	9	282	1.06e-119	352
Msa0362110	AT3G22420.4	58.214	280	116	1	11	290	80	358	1.94e-119	357
Msa0362110	AT3G22420.3	58.214	280	116	1	11	290	80	358	3.16e-119	357
Msa0362110	AT3G51630.1	61.538	273	105	0	12	284	11	283	1.60e-118	351
Msa0362110	AT3G51630.2	61.538	273	105	0	12	284	11	283	1.60e-118	351

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Find 48 sgRNAs with CRISPR-Local

Find 118 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGTGTCTCGCTCAACCA+AGG	0.579858	3_1:-52964200	Msa0362110:CDS
AAAGCATTTGATAATGAAGA+AGG	0.507018	3_1:-52964954	Msa0362110:CDS
AAAGTGTCTCGCTCAACCAA+GGG	0.644670	3_1:-52964199	Msa0362110:CDS
AAATGTTTCTGAGTCTTTGT+CGG	0.423160	3_1:+52965052	None:intergenic
AAGAAGGAATAGAAGTAGCA+TGG	0.500540	3_1:-52964938	Msa0362110:CDS
AAGGGCTAGACCTTCTGCTG+AGG	0.666215	3_1:-52964181	Msa0362110:CDS
AATGTATATTAGGTTAAGAT+TGG	0.380565	3_1:-52964495	Msa0362110:intron
ACATAAGCATGTTTCTATTA+AGG	0.226294	3_1:-52964718	Msa0362110:CDS
ACATTGTCACATTCACTATA+TGG	0.341582	3_1:+52964309	None:intergenic
ACTGATCCAACCGGAAGATA+TGG	0.362153	3_1:-52965023	Msa0362110:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TAAAGTCTTTCAATCATTGC+AGG	+	chr3_1:52964314-52964333	None:intergenic	30.0%
TGAAACAGAAATAATGTACC+TGG	+	chr3_1:52964619-52964638	None:intergenic	30.0%
ACATCTTGAGATAAACAACA+TGG	+	chr3_1:52964654-52964673	None:intergenic	30.0%
ATGGTAGACATATACTCATT+TGG	-	chr3_1:52964827-52964846	Msa0362110:CDS	30.0%
TGGAATGTTAGTATTAGAGA+TGG	-	chr3_1:52964847-52964866	Msa0362110:CDS	30.0%
ACATTGTCACATTCACTATA+TGG	+	chr3_1:52964887-52964906	None:intergenic	30.0%
TGTTGCAAAGATATACAAGA+AGG	-	chr3_1:52964904-52964923	Msa0362110:CDS	30.0%
TTTATCTTGTTCAAGGATTG+CGG	+	chr3_1:52964947-52964966	None:intergenic	30.0%
TAAAGTCTTTCAATCATTGC+AGG	+	chr3_1:52964314-52964333	None:intergenic	30.0%
TGAAACAGAAATAATGTACC+TGG	+	chr3_1:52964619-52964638	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_1	gene	52964116	52965099	52964116	ID=Msa0362110;Name=Msa0362110
chr3_1	mRNA	52964116	52965099	52964116	ID=Msa0362110-mRNA-1;Parent=Msa0362110;Name=Msa0362110-mRNA-1;_AED=0.23;_eAED=0.23;_QI=0\|0\|0\|1\|1\|1\|2\|0\|297
chr3_1	exon	52964596	52965099	52964596	ID=Msa0362110-mRNA-1:exon:9599;Parent=Msa0362110-mRNA-1
chr3_1	exon	52964116	52964505	52964116	ID=Msa0362110-mRNA-1:exon:9598;Parent=Msa0362110-mRNA-1
chr3_1	CDS	52964596	52965099	52964596	ID=Msa0362110-mRNA-1:cds;Parent=Msa0362110-mRNA-1
chr3_1	CDS	52964116	52964505	52964116	ID=Msa0362110-mRNA-1:cds;Parent=Msa0362110-mRNA-1

Gene Sequence

>Msa0362110

ATGCCAAGTGTTCAACAAAATCCATCCGACAAAGACTCAGAAACATTTGTTGAAACTGATCCAACCGGAAGATATGGAAGATACGATGAATTACTTGGAGCTGGTGCTTGTAAAAAAGTCTATAAAGCATTTGATAATGAAGAAGGAATAGAAGTAGCATGGAATCAAGTTAAGCTCAGAAATTTCAGCAATGATCCTGCAATGATTGAAAGACTTTACTCTGAAGTGAGACTGCTTAAAAACATGACAAACAAAAACATCATTGCACTTTACAGTGTTTGGAGAGACAAGGAGCATAACACACTGAATTTCATCACAGAGGTTTGTACAAGTGGGAATTTGAGGGAGTATAGGAAGAAACATAAGCATGTTTCTATTAAGGCTTTGAAGAAGTGGTCTAAGAAAATTTTGGAAGGGTTGAATTATTTACATGTGCATGATCCTTGTATCATTCATAGAGACCTCAACTGCAGTAATGTTTTTGTCAATGGAAATACTGGCCAGGTTAAGATTGGTGACTTAGGTTTGGCAGCAATTGTGGGAAAGAATCATTCAGCGCATTCAGTTCTAGGCACACCGGAATTTATGGCACCAGAGCTATATGAAGAGGATTACACAGAAATGGTAGACATATACTCATTTGGAATGTTAGTATTAGAGATGGTGACACTAGAGATTCCATATAGTGAATGTGACAATGTTGCAAAGATATACAAGAAGGTGACTTCAGGTGTGAGACCGCAATCCTTGAACAAGATAAAAGATGTAGAAGTGAAGACTTTCATCGAAAAGTGTCTCGCTCAACCAAGGGCTAGACCTTCTGCTGAGGAATTGCTAAAGGATCCTTTTTTTGATGATGTAGAGTACGATGAAAACGACGATGTTGATTACTAA

Protein sequence

>Msa0362110

MPSVQQNPSDKDSETFVETDPTGRYGRYDELLGAGACKKVYKAFDNEEGIEVAWNQVKLRNFSNDPAMIERLYSEVRLLKNMTNKNIIALYSVWRDKEHNTLNFITEVCTSGNLREYRKKHKHVSIKALKKWSKKILEGLNYLHVHDPCIIHRDLNCSNVFVNGNTGQVKIGDLGLAAIVGKNHSAHSVLGTPEFMAPELYEEDYTEMVDIYSFGMLVLEMVTLEIPYSECDNVAKIYKKVTSGVRPQSLNKIKDVEVKTFIEKCLAQPRARPSAEELLKDPFFDDVEYDENDDVDY*