AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0364820


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0364820 MtrunA17_Chr7g0223471 59.574 141 42 2 1 141 35 160 5.12e-40 138
Msa0364820 MtrunA17_Chr2g0283141 72.222 72 20 0 3 74 45 116 1.23e-28 108
Msa0364820 MtrunA17_Chr4g0046231 70.423 71 18 2 32 100 1 70 1.18e-23 94.0
Msa0364820 MtrunA17_Chr6g0460691 58.333 60 25 0 5 64 24 83 7.29e-20 83.2
Msa0364820 MtrunA17_Chr7g0254251 56.667 60 26 0 3 62 18 77 5.38e-18 77.8
Msa0364820 MtrunA17_Chr5g0436661 45.161 62 34 0 13 74 33 94 1.51e-12 61.6
Msa0364820 MtrunA17_Chr1g0153171 37.363 91 52 1 13 98 90 180 3.88e-12 61.6
Msa0364820 MtrunA17_Chr4g0043891 41.935 62 36 0 13 74 8 69 7.45e-11 58.2
Msa0364820 MtrunA17_Chr4g0031341 41.935 62 36 0 13 74 25 86 9.57e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0364820 AT2G33880.1 65.823 79 27 0 1 79 43 121 1.04e-28 108
Msa0364820 AT2G33880.2 65.823 79 27 0 1 79 43 121 1.43e-28 108
Msa0364820 AT5G45980.1 57.500 80 33 1 3 82 45 123 5.53e-25 97.4
Msa0364820 AT5G17810.3 58.621 58 24 0 7 64 15 72 5.05e-18 78.2
Msa0364820 AT5G17810.1 58.621 58 24 0 7 64 15 72 5.17e-18 78.2
Msa0364820 AT5G17810.2 58.621 58 24 0 7 64 15 72 5.17e-18 78.2
Msa0364820 AT3G03660.1 58.182 55 23 0 10 64 30 84 6.77e-18 77.8
Msa0364820 AT3G03660.2 58.182 55 23 0 10 64 30 84 1.02e-17 77.8
Msa0364820 AT3G03660.4 58.182 55 23 0 10 64 30 84 1.85e-17 77.0
Msa0364820 AT3G03660.3 58.182 55 23 0 10 64 30 84 2.36e-17 77.0
Msa0364820 AT2G17950.1 41.111 90 50 2 9 97 34 121 1.70e-14 68.9
Msa0364820 AT3G11260.1 45.161 62 34 0 13 74 24 85 2.43e-12 61.6
Msa0364820 AT1G46480.2 46.774 62 33 0 13 74 90 151 3.72e-12 61.2
Msa0364820 AT5G05770.1 38.667 75 42 1 13 83 29 103 6.21e-12 59.3
Msa0364820 AT1G46480.1 46.774 62 33 0 13 74 90 151 1.21e-11 60.5

Find 30 sgRNAs with CRISPR-Local

Find 113 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTGCATTTGGTTGTGTTTC+TGG 0.090039 3_1:-58339216 None:intergenic
CGTGAACAATTACAAGAATT+TGG 0.113481 3_1:+58339105 Msa0364820:CDS
AACAATTCACGAACGTTAAA+AGG 0.254042 3_1:-58339736 None:intergenic
TGTTTCTGGTCTCTTGTTGT+AGG 0.261301 3_1:-58339202 None:intergenic
GAAAGATGATGAAGATGATT+TGG 0.341139 3_1:-58339277 None:intergenic
ATACAGATTTGCTCAAGCTT+TGG 0.364320 3_1:-58339021 None:intergenic
ATTTGTGTGAGAGAGTTATT+AGG 0.365608 3_1:-58339240 None:intergenic
GCAGTTCTATTTGACTCCTT+TGG 0.365617 3_1:+58339769 Msa0364820:CDS
GTGAACAATTACAAGAATTT+GGG 0.368349 3_1:+58339106 Msa0364820:CDS
TTTGACTCCTTTGGTACCCC+TGG 0.401442 3_1:+58339778 Msa0364820:CDS
TTTAACGTTCGTGAATTGTT+TGG 0.410393 3_1:+58339739 Msa0364820:CDS
GTTTCTGGTCTCTTGTTGTA+GGG 0.424258 3_1:-58339201 None:intergenic
TCAAGCTTTGGATTCCGTCT+TGG 0.432449 3_1:-58339009 None:intergenic
TTCTCAATCTCATCTCTTGG+TGG 0.448475 3_1:-58339078 None:intergenic
CCCATTCATCGGTTAGAACC+AGG 0.458907 3_1:-58339796 None:intergenic
ATCTTCTCAATCTCATCTCT+TGG 0.466304 3_1:-58339081 None:intergenic
TTACAAGAATTTGGGCAACT+TGG 0.466911 3_1:+58339114 Msa0364820:CDS
GAGTTATTAGGAGTTGCATT+TGG 0.477754 3_1:-58339228 None:intergenic
TATTATGATTTCACCGGGAG+AGG 0.484380 3_1:-58339316 None:intergenic
GATGATGAAGATGATTTGGA+AGG 0.542768 3_1:-58339273 None:intergenic
CACCATCTCTGAGTTGAAGA+GGG 0.555445 3_1:-58339052 None:intergenic
TCACCATCTCTGAGTTGAAG+AGG 0.563521 3_1:-58339053 None:intergenic
TTTCTGGTCTCTTGTTGTAG+GGG 0.566911 3_1:-58339200 None:intergenic
CTTCTCAACTAAAGCCAAGA+CGG 0.582479 3_1:+58338995 Msa0364820:CDS
GATGAAGATGATTTGGAAGG+AGG 0.596007 3_1:-58339270 None:intergenic
TTTGTCTGCTTATGGACTTG+CGG 0.603126 3_1:-58339167 None:intergenic
AGCCCTCTTCAACTCAGAGA+TGG 0.607398 3_1:+58339050 Msa0364820:CDS
CCATTCATCGGTTAGAACCA+GGG 0.622177 3_1:-58339795 None:intergenic
GTTAGAACCAGGGGTACCAA+AGG 0.626698 3_1:-58339785 None:intergenic
CATTCATCGGTTAGAACCAG+GGG 0.647474 3_1:-58339794 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAGTTCTAATTAAATACAAA+TGG + chr3_1:58339408-58339427 Msa0364820:intron 15.0%
!!! TAGTTCTAATTAAATACAAA+TGG + chr3_1:58339408-58339427 Msa0364820:intron 15.0%
!! TTATGTGTTATATTATTCCA+TGG + chr3_1:58339552-58339571 Msa0364820:intron 20.0%
!! TTATGTGTTATATTATTCCA+TGG + chr3_1:58339552-58339571 Msa0364820:intron 20.0%
! GTGAACAATTACAAGAATTT+GGG + chr3_1:58339106-58339125 Msa0364820:CDS 25.0%
! ATGACATATTATGATTTCAC+CGG - chr3_1:58339325-58339344 None:intergenic 25.0%
! AACTGAATTCAAATTCTAGT+TGG + chr3_1:58339440-58339459 Msa0364820:intron 25.0%
! TCAAAAATGTAAGTAAAGTC+TGG - chr3_1:58339485-58339504 None:intergenic 25.0%
!! GAACTAGGTAATTTAAAGTT+TGG - chr3_1:58339513-58339532 None:intergenic 25.0%
!!! TAATCACATTTTTTTCAGGA+AGG - chr3_1:58339535-58339554 None:intergenic 25.0%
!!! CACATAATCACATTTTTTTC+AGG - chr3_1:58339539-58339558 None:intergenic 25.0%
!! TAGTTTATGTTTGTGTTTTC+AGG + chr3_1:58339601-58339620 Msa0364820:intron 25.0%
! GTGAACAATTACAAGAATTT+GGG + chr3_1:58339106-58339125 Msa0364820:CDS 25.0%
! ATGACATATTATGATTTCAC+CGG - chr3_1:58339325-58339344 None:intergenic 25.0%
! AACTGAATTCAAATTCTAGT+TGG + chr3_1:58339440-58339459 Msa0364820:intron 25.0%
! TCAAAAATGTAAGTAAAGTC+TGG - chr3_1:58339485-58339504 None:intergenic 25.0%
!! GAACTAGGTAATTTAAAGTT+TGG - chr3_1:58339513-58339532 None:intergenic 25.0%
!!! TAATCACATTTTTTTCAGGA+AGG - chr3_1:58339535-58339554 None:intergenic 25.0%
!!! CACATAATCACATTTTTTTC+AGG - chr3_1:58339539-58339558 None:intergenic 25.0%
!! TAGTTTATGTTTGTGTTTTC+AGG + chr3_1:58339601-58339620 Msa0364820:intron 25.0%
CGTGAACAATTACAAGAATT+TGG + chr3_1:58339105-58339124 Msa0364820:CDS 30.0%
! TCTTTTCTTTTGTCTGCTTA+TGG - chr3_1:58339178-58339197 None:intergenic 30.0%
ATTTGTGTGAGAGAGTTATT+AGG - chr3_1:58339243-58339262 None:intergenic 30.0%
GAAAGATGATGAAGATGATT+TGG - chr3_1:58339280-58339299 None:intergenic 30.0%
TGACATATTATGATTTCACC+GGG - chr3_1:58339324-58339343 None:intergenic 30.0%
GTCATTAATGATCAACTTCA+AGG + chr3_1:58339340-58339359 Msa0364820:intron 30.0%
TAATATCAGCATTTGCAAAG+TGG + chr3_1:58339367-58339386 Msa0364820:intron 30.0%
!!! ATTTTTTTCAGGAAGGAACT+AGG - chr3_1:58339528-58339547 None:intergenic 30.0%
AAATGTAGGGTGATTACTAA+TGG + chr3_1:58339688-58339707 Msa0364820:intron 30.0%
AACAATTCACGAACGTTAAA+AGG - chr3_1:58339739-58339758 None:intergenic 30.0%
TTTAACGTTCGTGAATTGTT+TGG + chr3_1:58339739-58339758 Msa0364820:CDS 30.0%
CGTGAACAATTACAAGAATT+TGG + chr3_1:58339105-58339124 Msa0364820:CDS 30.0%
! TCTTTTCTTTTGTCTGCTTA+TGG - chr3_1:58339178-58339197 None:intergenic 30.0%
ATTTGTGTGAGAGAGTTATT+AGG - chr3_1:58339243-58339262 None:intergenic 30.0%
GAAAGATGATGAAGATGATT+TGG - chr3_1:58339280-58339299 None:intergenic 30.0%
TGACATATTATGATTTCACC+GGG - chr3_1:58339324-58339343 None:intergenic 30.0%
GTCATTAATGATCAACTTCA+AGG + chr3_1:58339340-58339359 Msa0364820:intron 30.0%
TAATATCAGCATTTGCAAAG+TGG + chr3_1:58339367-58339386 Msa0364820:intron 30.0%
!!! ATTTTTTTCAGGAAGGAACT+AGG - chr3_1:58339528-58339547 None:intergenic 30.0%
AAATGTAGGGTGATTACTAA+TGG + chr3_1:58339688-58339707 Msa0364820:intron 30.0%
AACAATTCACGAACGTTAAA+AGG - chr3_1:58339739-58339758 None:intergenic 30.0%
TTTAACGTTCGTGAATTGTT+TGG + chr3_1:58339739-58339758 Msa0364820:CDS 30.0%
! ATACAGATTTGCTCAAGCTT+TGG - chr3_1:58339024-58339043 None:intergenic 35.0%
ATCTTCTCAATCTCATCTCT+TGG - chr3_1:58339084-58339103 None:intergenic 35.0%
TTACAAGAATTTGGGCAACT+TGG + chr3_1:58339114-58339133 Msa0364820:CDS 35.0%
GAGTTATTAGGAGTTGCATT+TGG - chr3_1:58339231-58339250 None:intergenic 35.0%
! GATGATGAAGATGATTTGGA+AGG - chr3_1:58339276-58339295 None:intergenic 35.0%
ACTAAATGAAGTCATGCACA+TGG - chr3_1:58339585-58339604 None:intergenic 35.0%
TGTGTGTGTTTGTCAAGTTT+CGG + chr3_1:58339646-58339665 Msa0364820:intron 35.0%
AAGATGGTATGGCAAAATGT+AGG + chr3_1:58339674-58339693 Msa0364820:intron 35.0%
AGATGGTATGGCAAAATGTA+GGG + chr3_1:58339675-58339694 Msa0364820:intron 35.0%
! ATACAGATTTGCTCAAGCTT+TGG - chr3_1:58339024-58339043 None:intergenic 35.0%
ATCTTCTCAATCTCATCTCT+TGG - chr3_1:58339084-58339103 None:intergenic 35.0%
TTACAAGAATTTGGGCAACT+TGG + chr3_1:58339114-58339133 Msa0364820:CDS 35.0%
GAGTTATTAGGAGTTGCATT+TGG - chr3_1:58339231-58339250 None:intergenic 35.0%
! GATGATGAAGATGATTTGGA+AGG - chr3_1:58339276-58339295 None:intergenic 35.0%
ACTAAATGAAGTCATGCACA+TGG - chr3_1:58339585-58339604 None:intergenic 35.0%
TGTGTGTGTTTGTCAAGTTT+CGG + chr3_1:58339646-58339665 Msa0364820:intron 35.0%
AAGATGGTATGGCAAAATGT+AGG + chr3_1:58339674-58339693 Msa0364820:intron 35.0%
AGATGGTATGGCAAAATGTA+GGG + chr3_1:58339675-58339694 Msa0364820:intron 35.0%
CTTCTCAACTAAAGCCAAGA+CGG + chr3_1:58338995-58339014 Msa0364820:CDS 40.0%
TTCTCAATCTCATCTCTTGG+TGG - chr3_1:58339081-58339100 None:intergenic 40.0%
! GTGATGCTAGTGTCTTTTAC+TGG + chr3_1:58339136-58339155 Msa0364820:CDS 40.0%
TTTGTCTGCTTATGGACTTG+CGG - chr3_1:58339170-58339189 None:intergenic 40.0%
! TTTCTGGTCTCTTGTTGTAG+GGG - chr3_1:58339203-58339222 None:intergenic 40.0%
!! GTTTCTGGTCTCTTGTTGTA+GGG - chr3_1:58339204-58339223 None:intergenic 40.0%
!! TGTTTCTGGTCTCTTGTTGT+AGG - chr3_1:58339205-58339224 None:intergenic 40.0%
GTTGCATTTGGTTGTGTTTC+TGG - chr3_1:58339219-58339238 None:intergenic 40.0%
! GATGAAGATGATTTGGAAGG+AGG - chr3_1:58339273-58339292 None:intergenic 40.0%
TATTATGATTTCACCGGGAG+AGG - chr3_1:58339319-58339338 None:intergenic 40.0%
GTGTTTGTCAAGTTTCGGTA+AGG + chr3_1:58339651-58339670 Msa0364820:intron 40.0%
TCAAGTTTCGGTAAGGAAGA+TGG + chr3_1:58339658-58339677 Msa0364820:intron 40.0%
TTTCGGTAAGGAAGATGGTA+TGG + chr3_1:58339663-58339682 Msa0364820:intron 40.0%
GCAGTTCTATTTGACTCCTT+TGG + chr3_1:58339769-58339788 Msa0364820:CDS 40.0%
CTTCTCAACTAAAGCCAAGA+CGG + chr3_1:58338995-58339014 Msa0364820:CDS 40.0%
TTCTCAATCTCATCTCTTGG+TGG - chr3_1:58339081-58339100 None:intergenic 40.0%
! GTGATGCTAGTGTCTTTTAC+TGG + chr3_1:58339136-58339155 Msa0364820:CDS 40.0%
TTTGTCTGCTTATGGACTTG+CGG - chr3_1:58339170-58339189 None:intergenic 40.0%
! TTTCTGGTCTCTTGTTGTAG+GGG - chr3_1:58339203-58339222 None:intergenic 40.0%
!! GTTTCTGGTCTCTTGTTGTA+GGG - chr3_1:58339204-58339223 None:intergenic 40.0%
!! TGTTTCTGGTCTCTTGTTGT+AGG - chr3_1:58339205-58339224 None:intergenic 40.0%
GTTGCATTTGGTTGTGTTTC+TGG - chr3_1:58339219-58339238 None:intergenic 40.0%
! GATGAAGATGATTTGGAAGG+AGG - chr3_1:58339273-58339292 None:intergenic 40.0%
TATTATGATTTCACCGGGAG+AGG - chr3_1:58339319-58339338 None:intergenic 40.0%
GTGTTTGTCAAGTTTCGGTA+AGG + chr3_1:58339651-58339670 Msa0364820:intron 40.0%
TCAAGTTTCGGTAAGGAAGA+TGG + chr3_1:58339658-58339677 Msa0364820:intron 40.0%
TTTCGGTAAGGAAGATGGTA+TGG + chr3_1:58339663-58339682 Msa0364820:intron 40.0%
GCAGTTCTATTTGACTCCTT+TGG + chr3_1:58339769-58339788 Msa0364820:CDS 40.0%
!! TCAAGCTTTGGATTCCGTCT+TGG - chr3_1:58339012-58339031 None:intergenic 45.0%
! CACCATCTCTGAGTTGAAGA+GGG - chr3_1:58339055-58339074 None:intergenic 45.0%
! TCACCATCTCTGAGTTGAAG+AGG - chr3_1:58339056-58339075 None:intergenic 45.0%
GATCATCAAAAAGCCTCTCC+CGG + chr3_1:58339303-58339322 Msa0364820:CDS 45.0%
!! TACTAATGGTGCCGTATTCG+AGG + chr3_1:58339702-58339721 Msa0364820:intron 45.0%
!! TCAAGCTTTGGATTCCGTCT+TGG - chr3_1:58339012-58339031 None:intergenic 45.0%
! CACCATCTCTGAGTTGAAGA+GGG - chr3_1:58339055-58339074 None:intergenic 45.0%
! TCACCATCTCTGAGTTGAAG+AGG - chr3_1:58339056-58339075 None:intergenic 45.0%
GATCATCAAAAAGCCTCTCC+CGG + chr3_1:58339303-58339322 Msa0364820:CDS 45.0%
!! TACTAATGGTGCCGTATTCG+AGG + chr3_1:58339702-58339721 Msa0364820:intron 45.0%
AGCCCTCTTCAACTCAGAGA+TGG + chr3_1:58339050-58339069 Msa0364820:CDS 50.0%
ATGCACATGGCACATGTCCA+TGG - chr3_1:58339572-58339591 None:intergenic 50.0%
CCCTGCATGAACCTCGAATA+CGG - chr3_1:58339716-58339735 None:intergenic 50.0%
CCGTATTCGAGGTTCATGCA+GGG + chr3_1:58339713-58339732 Msa0364820:intron 50.0%
TTTGACTCCTTTGGTACCCC+TGG + chr3_1:58339778-58339797 Msa0364820:CDS 50.0%
!! GTTAGAACCAGGGGTACCAA+AGG - chr3_1:58339788-58339807 None:intergenic 50.0%
AGCCCTCTTCAACTCAGAGA+TGG + chr3_1:58339050-58339069 Msa0364820:CDS 50.0%
ATGCACATGGCACATGTCCA+TGG - chr3_1:58339572-58339591 None:intergenic 50.0%
CCCTGCATGAACCTCGAATA+CGG - chr3_1:58339716-58339735 None:intergenic 50.0%
CCGTATTCGAGGTTCATGCA+GGG + chr3_1:58339713-58339732 Msa0364820:intron 50.0%
TTTGACTCCTTTGGTACCCC+TGG + chr3_1:58339778-58339797 Msa0364820:CDS 50.0%
!! GTTAGAACCAGGGGTACCAA+AGG - chr3_1:58339788-58339807 None:intergenic 50.0%
GCCGTATTCGAGGTTCATGC+AGG + chr3_1:58339712-58339731 Msa0364820:intron 55.0%
!! ACCCCTGGTTCTAACCGATG+AGG + chr3_1:58339793-58339812 Msa0364820:CDS 55.0%
GCCGTATTCGAGGTTCATGC+AGG + chr3_1:58339712-58339731 Msa0364820:intron 55.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 58338976 58339813 58338976 ID=Msa0364820;Name=Msa0364820
chr3_1 mRNA 58338976 58339813 58338976 ID=Msa0364820-mRNA-1;Parent=Msa0364820;Name=Msa0364820-mRNA-1;_AED=0.34;_eAED=0.34;_QI=0|0|0|1|1|0.5|2|0|142
chr3_1 exon 58338976 58339324 58338976 ID=Msa0364820-mRNA-1:exon:11023;Parent=Msa0364820-mRNA-1
chr3_1 exon 58339734 58339813 58339734 ID=Msa0364820-mRNA-1:exon:11024;Parent=Msa0364820-mRNA-1
chr3_1 CDS 58338976 58339324 58338976 ID=Msa0364820-mRNA-1:cds;Parent=Msa0364820-mRNA-1
chr3_1 CDS 58339734 58339813 58339734 ID=Msa0364820-mRNA-1:cds;Parent=Msa0364820-mRNA-1
Gene Sequence

>Msa0364820

GGAGATGCTGAAGAGAGAACTTCTCAACTAAAGCCAAGACGGAATCCAAAGCTTGAGCAAATCTGTATTCTTGAAGCCCTCTTCAACTCAGAGATGGTGAATCCACCAAGAGATGAGATTGAGAAGATACGTGAACAATTACAAGAATTTGGGCAACTTGGTGATGCTAGTGTCTTTTACTGGTTTCAAAACCGCAAGTCCATAAGCAGACAAAAGAAAAGAATCCCCTACAACAAGAGACCAGAAACACAACCAAATGCAACTCCTAATAACTCTCTCACACAAATTAATACACCTCCTTCCAAATCATCTTCATCATCTTTCATTGATCATCAAAAAGCCTCTCCCGGGCCTTTTAACGTTCGTGAATTGTTTGGTGATGAAGCAGTTCTATTTGACTCCTTTGGTACCCCTGGTTCTAACCGATGA

Protein sequence

>Msa0364820

GDAEERTSQLKPRRNPKLEQICILEALFNSEMVNPPRDEIEKIREQLQEFGQLGDASVFYWFQNRKSISRQKKRIPYNKRPETQPNATPNNSLTQINTPPSKSSSSSFIDHQKASPGPFNVRELFGDEAVLFDSFGTPGSNR*