AlfalfaGEDB | Msa0366270

M. sativa cultivar zhongmu-4 / Msa0366270

KEGG: K07198|K12761
GO: GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009268 GO:0009628 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018107 GO:0018193 GO:0018209 GO:0018210 GO:0019538 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0140096 GO:1901564
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0366270	XP_013460408.1	94.808	443	23	0	1	443	1	443	0.0	871

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0366270	sp\|O22932\|CIPKB_ARATH	62.673	434	148	6	1	425	1	429	0.0	541

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0366270	A0A072UYE6	94.808	443	23	0	1	443	1	443	0.0	871

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0366270	MtrunA17_Chr3g0107051	94.808	443	23	0	1	443	1	443	0.0	871
Msa0366270	MtrunA17_Chr5g0432491	67.536	422	127	6	17	434	19	434	0.0	572
Msa0366270	MtrunA17_Chr2g0284031	52.061	461	180	5	15	435	25	484	7.24e-166	476
Msa0366270	MtrunA17_Chr8g0379091	51.549	452	160	6	17	417	22	465	7.12e-159	458
Msa0366270	MtrunA17_Chr7g0244561	54.545	418	175	5	7	418	2	410	2.93e-151	436
Msa0366270	MtrunA17_Chr2g0284011	47.775	427	198	4	15	417	6	431	2.55e-142	414
Msa0366270	MtrunA17_Chr7g0244521	47.775	427	197	6	15	417	6	430	1.00e-137	403
Msa0366270	MtrunA17_Chr4g0013131	49.526	422	192	7	15	417	6	425	7.81e-137	400
Msa0366270	MtrunA17_Chr5g0432471	44.898	441	203	7	17	417	8	448	1.90e-129	382
Msa0366270	MtrunA17_Chr4g0063881	46.635	416	196	8	18	411	8	419	8.14e-129	380

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0366270	AT2G30360.1	62.673	434	148	6	1	425	1	429	0.0	541
Msa0366270	AT5G01820.1	59.397	431	159	5	17	438	18	441	1.45e-173	494
Msa0366270	AT4G18700.1	53.829	444	169	5	5	417	14	452	1.94e-166	477
Msa0366270	AT5G45810.1	51.370	438	172	4	17	417	24	457	8.85e-159	457
Msa0366270	AT1G29230.1	50.455	440	187	5	8	417	61	499	2.27e-152	442
Msa0366270	AT5G45820.1	50.739	406	192	4	20	417	11	416	1.01e-148	431
Msa0366270	AT2G34180.1	47.534	446	199	5	2	417	39	479	7.63e-146	425
Msa0366270	AT2G38490.1	53.415	410	169	6	15	417	22	416	2.31e-141	411
Msa0366270	AT4G30960.1	49.420	431	195	4	4	417	7	431	9.28e-141	410
Msa0366270	AT5G01810.1	49.383	405	198	3	20	418	11	414	8.88e-138	402

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Find 110 sgRNAs with CRISPR-Local

Find 272 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTACTTGATACAAACC+CGG	0.662431	3_1:-60898869	Msa0366270:CDS
AAAATTTAATGGTTATGTAT+AGG	0.330011	3_1:-60898957	Msa0366270:CDS
AAAGTTGATGTTTGGTCATG+TGG	0.551578	3_1:-60899027	Msa0366270:CDS
AAATGGGAATTTAAAAGTTT+CGG	0.314941	3_1:-60899169	Msa0366270:CDS
AAATTTCATGAGGAAGATTA+TGG	0.279779	3_1:-60898781	Msa0366270:CDS
AAGGAAGATATCCTGCCGCA+AGG	0.646887	3_1:+60898989	None:intergenic
AATAGGAGTGGTGGAGGGAA+TGG	0.444429	3_1:-60898575	Msa0366270:CDS
AATGTGGGGTAGAGTTAGAA+GGG	0.490976	3_1:-60898534	Msa0366270:CDS
AATTTAAAAGTTTCGGATTT+CGG	0.237309	3_1:-60899162	Msa0366270:CDS
AATTTGTTATTAGATGAAAA+TGG	0.274844	3_1:-60899186	Msa0366270:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTGAATTTGTCTGGATTGTT+CGG	-	chr3_1:60899278-60899297	Msa0366270:CDS	30.0%
TTGAATTTGTCTGGATTGTT+CGG	-	chr3_1:60899278-60899297	Msa0366270:CDS	30.0%
TACGAATTAGGAAGAGTTGT+CGG	-	chr3_1:60898378-60898397	Msa0366270:CDS	35.0%
TTGGTTATTGTCATTCACGA+GGG	-	chr3_1:60898709-60898728	Msa0366270:CDS	35.0%
TAGAGTATGAAGCAATCCAT+CGG	+	chr3_1:60898838-60898857	None:intergenic	35.0%
ACATGACCAAACATCAACTT+TGG	+	chr3_1:60898919-60898938	None:intergenic	35.0%
CATGAGGAAGATTATGGATA+CGG	-	chr3_1:60899170-60899189	Msa0366270:CDS	35.0%
ATGAGGAAGATTATGGATAC+GGG	-	chr3_1:60899171-60899190	Msa0366270:CDS	35.0%
GTGTAAATGTGATAGATCAG+AGG	-	chr3_1:60899211-60899230	Msa0366270:CDS	35.0%
CAATCCAGACAAATTCAAAC+CGG	+	chr3_1:60899276-60899295	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_1	gene	60898318	60899649	60898318	ID=Msa0366270;Name=Msa0366270
chr3_1	mRNA	60898318	60899649	60898318	ID=Msa0366270-mRNA-1;Parent=Msa0366270;Name=Msa0366270-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|443
chr3_1	exon	60898318	60899649	60898318	ID=Msa0366270-mRNA-1:exon:11782;Parent=Msa0366270-mRNA-1
chr3_1	CDS	60898318	60899649	60898318	ID=Msa0366270-mRNA-1:cds;Parent=Msa0366270-mRNA-1

Gene Sequence

>Msa0366270

ATGCCGGAGATCGAACGAGCGTCACCGGATGTAGCAGCGGTAACAGCTATTTTTGGAAAATACGAATTAGGAAGAGTTGTCGGTTGTGGCGCTTTTGCAAAAGTGTACTATGCACGTAACGTTCAAACGGGACAAAGCGTGGCTGTGAAGATAATCAACAAAAAGAAACTCAAAGGCACAGGTCTAGCCGAAAATGTGAAACTCGAAATCACAATCATGAGTCGTCTTCATCATCCTCATATTGTTCGTCTTCACGAAGTTCTTGCCACCAAGACGAAAATCTATTTTGTTATGGACTTTGTTCGTGGTGGCGAGCTCTTCGCGAAGATATCAAAGGGTAGGTTTAGCGAAGATCTTAGCCGGAGATATTTTCATCAATTAATCTCGGCTGTTGGTTATTGTCATTCACGAGGGGTTTTTCATCGGGATCTTAAGCCGGAGAATTTGTTATTAGATGAAAATGGGAATTTAAAAGTTTCGGATTTCGGGTTAAGTGCGGTCACAGATCAGATCCGATCCGATGGATTGCTTCATACTCTATGTGGAACCCCTGCTTACGTGGCACCCGAGGTTCTTGCTAAGCAAGGATATGATGGTGCCAAAGTTGATGTTTGGTCATGTGGCGTGGTTTTGTTTGTCCTTGCGGCAGGATATCTTCCTTTTAATGATCAAAATTTAATGGTTATGTATAGGAAAATTTATAAAGGTGAATACAAGTGTCCACGTTGGATGTCGCCGGGTCTCCAAAGATTTTTGTCAAAATTACTTGATACAAACCCGGAAACTCGGATCACAATTGATGGTATGACCCGGGATCCATGGTTTAGAAAAGGGTACAAAGAGATAAAATTTCATGAGGAAGATTATGGATACGGGCACGAGTTTGGGTTTAGGTGTAAATGTGATAGATCAGAGGTATTGAATTTGAATGCGTTTGATATTATATCATTTTCTTCCGGTTTGAATTTGTCTGGATTGTTCGGGAAATTGGATGGGACTGAGCGGTTTGTTGTAAGGGAGTCGCCGAAGAGGATTTTAAAGGTGGTGGAGGAAATAGGAGTGGTGGAGGGAATGGCGTTGAGGTTGAAGAAAGAATGTGGGGTAGAGTTAGAAGGGTTGAATGGTAAATTTGGAATTGAGATTGAGGTTTACCAGTTAACGGAGGAGGTGGTGATGGTTGAGGTACGGAGGAGAGGAAATGATGCAGCGACTCTTAGTGATTTGTGGAATAATAAGCTTAAACCTCAGTTGCTACTATCTAGTGCAACGACATCACATTTAGATGAACGTGAACCTGAATCTTCACATTCGAATGAACGTGAACCTGAATAA

Protein sequence

>Msa0366270

MPEIERASPDVAAVTAIFGKYELGRVVGCGAFAKVYYARNVQTGQSVAVKIINKKKLKGTGLAENVKLEITIMSRLHHPHIVRLHEVLATKTKIYFVMDFVRGGELFAKISKGRFSEDLSRRYFHQLISAVGYCHSRGVFHRDLKPENLLLDENGNLKVSDFGLSAVTDQIRSDGLLHTLCGTPAYVAPEVLAKQGYDGAKVDVWSCGVVLFVLAAGYLPFNDQNLMVMYRKIYKGEYKCPRWMSPGLQRFLSKLLDTNPETRITIDGMTRDPWFRKGYKEIKFHEEDYGYGHEFGFRCKCDRSEVLNLNAFDIISFSSGLNLSGLFGKLDGTERFVVRESPKRILKVVEEIGVVEGMALRLKKECGVELEGLNGKFGIEIEVYQLTEEVVMVEVRRRGNDAATLSDLWNNKLKPQLLLSSATTSHLDEREPESSHSNEREPE*